Rroxscaffold_2G00086330

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002611
Physical Location & Seq
Forward (+)
8557533 .. 8560445
2913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086330.1

Sequence Viewer

Length: 1479 bp
ATGTGCAAAGCAGGGGACTTTGAGAGGTGTGATTACAGAACAGAGTGGAAGGTGGGATACAGGGTTTTGAGTAATGTAGAGAAATTGAAGAACTCGATTTTCAAGATGAACTCATGGTTGTTGATCATGAGGGCAATCGTGACAGCTTTGGTTTGGGCCTCTGCTGTTCAGTTGATTGGGATTTGGTTAATTAGAGGGCCAAGAGTGTTCAAGGTTTGGCCTCCTTGTAATCAATTAGATTTGCATGTTGCTTCTGAATTGCCGAAGCTTCCAATCCCAGTTTTCCCACCAAAGAGGGTTTACAAGAATAATGGGTATCTAATGGTTTCTTGCAATGGAGGATTGAATCAAATGCGAGCAGCGATTTGTGATATGGTTGCAATTGCCAGATATTTGAATGTGACTCTTATTGTTCCAGAGCTGGATAAGAACTCATTTTGGGCTGCGAGTGAGTTTCAAGACATTTTCGATGTTAATCATTTCATTAGATCATTGAGAGATGAGGTTCGGATACTAAAGAAACTGCCCCCGAAGCTCAACAAGAGAGCAGTATACTCATTGCCACCTGTTAGTTGGTCCAACATTTCCTACTACATTCATCAGATCCTACCGCTGGTGCGAAAGTATAAAGTAGTGCATTTGAACAAAACCGATGCTCGAGTGGCTAACAATGGGCTACCTCTTGAGATTCAGAAGTTGAGATGCAGAGTCAATTTCCATGGTCTGAGATTTACTTCCCAGATAGAGGAAACCGGTCGAAAGCTTGTCAGAATTTTAAGAGAAAAAGGCCCTTTTCTGGTGCTTCATCTTAGATATGAAATGGACATGTTGGCATTCTCTGGCTGCAGCCATGGTTGCACCAATGATGAGGTGGAAGAACTCAACACAATGAGATATGCTAATCCATTGTGGAAGGAAAAAGACATTGATTCTGAAATGAAAAGGGTAGAAGGCTTGTGCCCTCTAACACCAGAGGAAACTGCTCTGGTATTGGTTGCATTAGGTATAGATCACAATGTTCAGATTTATATTGCTGCCGGAGATATATACGGTGGAGATCGAAGAATGGAAGGTATGAAAGCTGCTTTTCCTAATGTGGTCAGGAAAGAGACGCTGCTTGAGCCTTCGGAATTGAGGTTCTTCCCAAACCACTCTTCTCAAATGGCAGCATTGGACTACCTTGTGTCTCTGGAGAGTGACATTTTCGTTCCCACATACGATGGAAACATGGCCAAAGTTGTTGAAGGCCATCGCAGGTTCTTAGGTTTCAAAAAGACTATTTTACTAGACAGAAGGCTACTCCTTGGCTTGATAGATCAGTTTACTAGTGGATCATTGAGCTGGGATGAGTTCTCTTCCATTGTGAAGGAAGCTCATGCTGATCGGATGGGGAGCCCGAAAAACGAGTTTCTATTGTTGATAAACCAAAAGAAGAAGATTACTTCTACTCAAATCCACATGAATGTTTGCAACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

492

Amino Acids

56.45

Weight (kDa)

9.18

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 106 - 420 1.6e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1245
AccI GTMKAC 1 cut(s) 552
AciI CCGC 1 cut(s) 611
AclWI GGATC 2 cut(s) 598, 1339
AcoI YGGCCR 1 cut(s) 1230
AcsI RAATTY 1 cut(s) 771
AfiI CCNNNNNNNGG 3 cut(s) 613, 745, 796
AflIII ACRYGT 1 cut(s) 825
AgeI ACCGGT 1 cut(s) 752
AgsI TTSAA 9 cut(s) 88, 103, 211, 346, 397, 458, 643, 1244, 1270
AhlI ACTAGT 1 cut(s) 1325
AjuI GAANNNNNNNTTGG 2 cut(s) 1138, 1170
AloI GAACNNNNNNTCC 2 cut(s) 1121, 1153
AluBI AGCT 8 cut(s) 146, 268, 421, 535, 763, 1082, 1341, 1373
AluI AGCT 8 cut(s) 146, 268, 421, 535, 763, 1082, 1341, 1373
Alw26I GTCTC 2 cut(s) 1103, 1191
AlwI GGATC 2 cut(s) 598, 1339
Ama87I CYCGRG 1 cut(s) 657
AoxI GGCC 6 cut(s) 156, 197, 218, 787, 1230, 1246
ApeKI GCWGC 8 cut(s) 359, 443, 843, 846, 1034, 1082, 1114, 1166
ApoI RAATTY 1 cut(s) 771
AsiGI ACCGGT 1 cut(s) 752
AspS9I GGNCC 4 cut(s) 156, 197, 576, 788
AvaI CYCGRG 1 cut(s) 657
AvaII GGWCC 1 cut(s) 576
BaeGI GKGCMC 1 cut(s) 962
BalI TGGCCA 1 cut(s) 1232
BanII GRGCYC 1 cut(s) 1397
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
BbvI GCAGC 8 cut(s) 371, 430, 830, 858, 1021, 1069, 1101, 1178
BccI CCATC 3 cut(s) 1214, 1257, 1381
BciVI GTATCC 2 cut(s) 50, 504
BclI TGATCA 1 cut(s) 123
BcoDI GTCTC 2 cut(s) 1103, 1191
BcuI ACTAGT 1 cut(s) 1325
BfaI CTAG 2 cut(s) 1286, 1326
BfmI CTRYAG 1 cut(s) 844
BfuAI ACCTGC 1 cut(s) 1245
BfuI GTATCC 2 cut(s) 50, 504
BisI GCNGC 8 cut(s) 360, 444, 844, 847, 1035, 1083, 1115, 1167
BlsI GCNGC 8 cut(s) 361, 445, 845, 848, 1036, 1084, 1116, 1168
Bme18I GGWCC 1 cut(s) 576
BmeT110I CYCGRG 1 cut(s) 657
BmgT120I GGNCC 4 cut(s) 156, 197, 576, 788
BmiI GGNNCC 1 cut(s) 1394
BmrI ACTGGG 1 cut(s) 272
BmsI GCATC 2 cut(s) 643, 692
BmuI ACTGGG 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 1211
BpuEI CTTGAG 2 cut(s) 704, 1139
BsaBI GATNNNNATC 1 cut(s) 474
BsaJI CCNNGG 3 cut(s) 718, 850, 1303
BsaWI WCCGGW 1 cut(s) 752
Bsc4I CCNNNNNNNGG 3 cut(s) 613, 745, 796
Bse118I RCCGGY 1 cut(s) 752
Bse1I ACTGG 1 cut(s) 278
Bse3DI GCAATG 2 cut(s) 340, 557
Bse8I GATNNNNATC 1 cut(s) 474
BseDI CCNNGG 3 cut(s) 718, 850, 1303
BseGI GGATG 2 cut(s) 1351, 1392
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 3 cut(s) 613, 745, 796
BseMI GCAATG 2 cut(s) 340, 557
BseMII CTCAG 1 cut(s) 716
BseNI ACTGG 1 cut(s) 278
BseSI GKGCMC 1 cut(s) 962
BseXI GCAGC 8 cut(s) 371, 430, 830, 858, 1021, 1069, 1101, 1178
BseYI CCCAGC 1 cut(s) 1341
Bsh1285I CGRYCG 1 cut(s) 757
BshFI GGCC 6 cut(s) 158, 199, 220, 789, 1232, 1248
BshTI ACCGGT 1 cut(s) 752
BsiEI CGRYCG 1 cut(s) 757
BsiHKCI CYCGRG 1 cut(s) 657
BsiSI CCGG 2 cut(s) 753, 1038
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 3 cut(s) 613, 745, 796
BsmAI GTCTC 2 cut(s) 1103, 1191
BsmBI CGTCTC 1 cut(s) 1103
BsmFI GGGAC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 833
BsnI GGCC 6 cut(s) 158, 199, 220, 789, 1232, 1248
BsoBI CYCGRG 1 cut(s) 657
Bsp1286I GDGCHC 2 cut(s) 962, 1397
Bsp143I GATC 8 cut(s) 123, 488, 603, 1009, 1057, 1315, 1331, 1381
Bsp19I CCATGG 2 cut(s) 718, 850
BspACI CCGC 1 cut(s) 611
BspANI GGCC 6 cut(s) 158, 199, 220, 789, 1232, 1248
BspCNI CTCAG 1 cut(s) 717
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 1 cut(s) 1394
BspMAI CTGCAG 1 cut(s) 848
BspMI ACCTGC 1 cut(s) 1245
BspPI GGATC 2 cut(s) 598, 1339
BsrDI GCAATG 2 cut(s) 340, 557
BsrFI RCCGGY 1 cut(s) 752
BsrI ACTGG 1 cut(s) 278
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 3 cut(s) 718, 850, 1303
BssMI GATC 8 cut(s) 123, 488, 603, 1009, 1057, 1315, 1331, 1381
BssNAI GTATAC 1 cut(s) 553
BssT1I CCWWGG 3 cut(s) 718, 850, 1303
Bst1107I GTATAC 1 cut(s) 553
Bst4CI ACNGT 2 cut(s) 1052, 1475
Bst6I CTCTTC 2 cut(s) 1159, 1360
BstC8I GCNNGC 1 cut(s) 357
BstDEI CTNAG 3 cut(s) 725, 809, 1261
BstDSI CCRYGG 2 cut(s) 718, 850
BstF5I GGATG 2 cut(s) 1351, 1392
BstKTI GATC 8 cut(s) 126, 491, 606, 1012, 1060, 1318, 1334, 1384
BstMAI GTCTC 2 cut(s) 1103, 1191
BstMBI GATC 8 cut(s) 123, 488, 603, 1009, 1057, 1315, 1331, 1381
BstMCI CGRYCG 1 cut(s) 757
BstMWI GCNNNNNNNGC 4 cut(s) 532, 662, 855, 1120
BstNSI RCATGY 2 cut(s) 248, 829
BstSFI CTRYAG 1 cut(s) 844
BstSLI GKGCMC 1 cut(s) 962
BstV1I GCAGC 8 cut(s) 371, 430, 830, 858, 1021, 1069, 1101, 1178
BstX2I RGATCY 1 cut(s) 603
BstYI RGATCY 1 cut(s) 603
BstZ17I GTATAC 1 cut(s) 553
BsuI GTATCC 2 cut(s) 50, 504
BsuRI GGCC 6 cut(s) 158, 199, 220, 789, 1232, 1248
BtgI CCRYGG 2 cut(s) 718, 850
BtgZI GCGATG 1 cut(s) 1235
BtsCI GGATG 2 cut(s) 1351, 1392
BveI ACCTGC 1 cut(s) 1245
Cac8I GCNNGC 1 cut(s) 357
CciI TCATGA 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 752
Cfr13I GGNCC 4 cut(s) 156, 197, 576, 788
CseI GACGC 1 cut(s) 1120
CspAI ACCGGT 1 cut(s) 752
CviAII CATG 9 cut(s) 114, 127, 245, 719, 826, 851, 1228, 1376, 1459
DdeI CTNAG 3 cut(s) 725, 809, 1261
DpnI GATC 8 cut(s) 125, 490, 605, 1011, 1059, 1317, 1333, 1383
DpnII GATC 8 cut(s) 123, 488, 603, 1009, 1057, 1315, 1331, 1381
EaeI YGGCCR 1 cut(s) 1230
Eam1104I CTCTTC 2 cut(s) 1159, 1360
EarI CTCTTC 2 cut(s) 1159, 1360
Eco130I CCWWGG 3 cut(s) 718, 850, 1303
Eco24I GRGCYC 1 cut(s) 1397
Eco47I GGWCC 1 cut(s) 576
Eco88I CYCGRG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 788
EcoT14I CCWWGG 3 cut(s) 718, 850, 1303
EcoT38I GRGCYC 1 cut(s) 1397
ErhI CCWWGG 3 cut(s) 718, 850, 1303
Esp3I CGTCTC 1 cut(s) 1103
FaeI CATG 9 cut(s) 117, 130, 248, 722, 829, 854, 1231, 1379, 1462
FaqI GGGAC 1 cut(s) 29
FatI CATG 9 cut(s) 113, 126, 244, 718, 825, 850, 1227, 1375, 1458
FbaI TGATCA 1 cut(s) 123
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 8 cut(s) 360, 444, 844, 847, 1035, 1083, 1115, 1167
FokI GGATG 2 cut(s) 1358, 1399
FriOI GRGCYC 1 cut(s) 1397
Fsp4HI GCNGC 8 cut(s) 360, 444, 844, 847, 1035, 1083, 1115, 1167
FspBI CTAG 2 cut(s) 1286, 1326
GluI GCNGC 8 cut(s) 360, 444, 844, 847, 1035, 1083, 1115, 1167
GsaI CCCAGC 1 cut(s) 1345
GsuI CTGGAG 1 cut(s) 1211
HaeIII GGCC 6 cut(s) 158, 199, 220, 789, 1232, 1248
HapII CCGG 2 cut(s) 753, 1038
HgaI GACGC 1 cut(s) 1120
Hin1II CATG 9 cut(s) 117, 130, 248, 722, 829, 854, 1231, 1379, 1462
HindIII AAGCTT 2 cut(s) 266, 761
HinfI GANTC 5 cut(s) 346, 403, 688, 708, 929
HpaII CCGG 2 cut(s) 753, 1038
Hpy166II GTNNAC 3 cut(s) 301, 553, 1323
Hpy188III TCNNGA 8 cut(s) 103, 127, 139, 416, 458, 683, 1102, 1190
Hpy8I GTNNAC 3 cut(s) 301, 553, 1323
HpyAV CCTTC 8 cut(s) 43, 907, 944, 1064, 1134, 1238, 1287, 1360
HpyCH4III ACNGT 2 cut(s) 1052, 1475
HpyF10VI GCNNNNNNNGC 4 cut(s) 532, 662, 855, 1120
HpyF3I CTNAG 3 cut(s) 725, 809, 1261
Hsp92II CATG 9 cut(s) 117, 130, 248, 722, 829, 854, 1231, 1379, 1462
Ksp22I TGATCA 1 cut(s) 123
Kzo9I GATC 8 cut(s) 123, 488, 603, 1009, 1057, 1315, 1331, 1381
LmnI GCTCC 1 cut(s) 1392
Lsp1109I GCAGC 8 cut(s) 371, 430, 830, 858, 1021, 1069, 1101, 1178
LweI GCATC 2 cut(s) 643, 692
MaeI CTAG 2 cut(s) 1286, 1326
MaeIII GTNAC 3 cut(s) 139, 400, 1196
MalI GATC 8 cut(s) 125, 490, 605, 1011, 1059, 1317, 1333, 1383
MboI GATC 8 cut(s) 123, 488, 603, 1009, 1057, 1315, 1331, 1381
MboII GAAGA 8 cut(s) 100, 887, 1074, 1132, 1146, 1347, 1444, 1447
MfeI CAATTG 1 cut(s) 381
MflI RGATCY 1 cut(s) 603
MhlI GDGCHC 2 cut(s) 962, 1397
MlsI TGGCCA 1 cut(s) 1232
MluCI AATT 8 cut(s) 83, 189, 233, 257, 381, 712, 771, 1130
MluNI TGGCCA 1 cut(s) 1232
MlyI GAGTC 2 cut(s) 397, 717
MmeI TCCRAC 1 cut(s) 603
Mox20I TGGCCA 1 cut(s) 1232
MscI TGGCCA 1 cut(s) 1232
MseI TTAA 3 cut(s) 188, 474, 776
MslI CAYNNNNRTG 1 cut(s) 1461
Msp20I TGGCCA 1 cut(s) 1232
MspA1I CMGCKG 1 cut(s) 613
MspI CCGG 2 cut(s) 753, 1038
MunI CAATTG 1 cut(s) 381
Mva1269I GAATGC 1 cut(s) 833
MwoI GCNNNNNNNGC 4 cut(s) 532, 662, 855, 1120
NcoI CCATGG 2 cut(s) 718, 850
NdeII GATC 8 cut(s) 123, 488, 603, 1009, 1057, 1315, 1331, 1381
NlaIII CATG 9 cut(s) 117, 130, 248, 722, 829, 854, 1231, 1379, 1462
NlaIV GGNNCC 1 cut(s) 1394
NmuCI GTSAC 3 cut(s) 139, 400, 1196
NspI RCATGY 2 cut(s) 248, 829
PaeR7I CTCGAG 1 cut(s) 657
PagI TCATGA 1 cut(s) 126
PciI ACATGT 1 cut(s) 825
PctI GAATGC 1 cut(s) 833
PfeI GAWTC 3 cut(s) 346, 688, 929
PinAI ACCGGT 1 cut(s) 752
PkrI GCNGC 8 cut(s) 361, 445, 845, 848, 1036, 1084, 1116, 1168
PleI GAGTC 2 cut(s) 397, 716
PpsI GAGTC 2 cut(s) 397, 716
PscI ACATGT 1 cut(s) 825
PspFI CCCAGC 1 cut(s) 1341
PspN4I GGNNCC 1 cut(s) 1394
PspPI GGNCC 4 cut(s) 156, 197, 576, 788
PspXI VCTCGAGB 1 cut(s) 657
PstI CTGCAG 1 cut(s) 848
PsuI RGATCY 1 cut(s) 603
RseI CAYNNNNRTG 1 cut(s) 1461
SaqAI TTAA 3 cut(s) 188, 474, 776
SatI GCNGC 8 cut(s) 360, 444, 844, 847, 1035, 1083, 1115, 1167
Sau3AI GATC 8 cut(s) 123, 488, 603, 1009, 1057, 1315, 1331, 1381
Sau96I GGNCC 4 cut(s) 156, 197, 576, 788
SchI GAGTC 2 cut(s) 397, 717
SduI GDGCHC 2 cut(s) 962, 1397
SfaNI GCATC 2 cut(s) 643, 692
SfcI CTRYAG 1 cut(s) 844
Sfr274I CTCGAG 1 cut(s) 657
SinI GGWCC 1 cut(s) 576
SlaI CTCGAG 1 cut(s) 657
SmiMI CAYNNNNRTG 1 cut(s) 1461
SmlI CTYRAG 3 cut(s) 657, 683, 1118
SmoI CTYRAG 3 cut(s) 657, 683, 1118
SpeI ACTAGT 1 cut(s) 1325
Sse9I AATT 8 cut(s) 83, 189, 233, 257, 381, 712, 771, 1130
SsiI CCGC 1 cut(s) 611
SspMI CTAG 2 cut(s) 1286, 1326
StyI CCWWGG 3 cut(s) 718, 850, 1303
TaaI ACNGT 2 cut(s) 1052, 1475
TaqI TCGA 5 cut(s) 95, 468, 658, 757, 1060
TasI AATT 8 cut(s) 83, 189, 233, 257, 381, 712, 771, 1130
TfiI GAWTC 3 cut(s) 346, 688, 929
Tru1I TTAA 3 cut(s) 188, 474, 776
Tru9I TTAA 3 cut(s) 188, 474, 776
TseFI GTSAC 3 cut(s) 139, 400, 1196
TseI GCWGC 8 cut(s) 359, 443, 843, 846, 1034, 1082, 1114, 1166
Tsp45I GTSAC 3 cut(s) 139, 400, 1196
TspDTI ATGAA 8 cut(s) 122, 472, 587, 794, 831, 953, 1091, 1475
VpaK11BI GGWCC 1 cut(s) 576
XapI RAATTY 1 cut(s) 771
XceI RCATGY 2 cut(s) 248, 829
XcmI CCANNNNNNNNNTGG 2 cut(s) 570, 868
XhoI CTCGAG 1 cut(s) 657
XmiI GTMKAC 1 cut(s) 552
XspI CTAG 2 cut(s) 1286, 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.