AT3G02530
TCP Family

assists the folding of proteins upon ATP hydrolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003345
Physical Location & Seq
Reverse (-)
528526 .. 532612
4087 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G02530.1

Sequence Viewer

Length: 1608 bp
ATGTCTGTGCGTGTACTGAATCCTAATGCTGAGGTGCTCAACAAATCGGCCGCGCTTCATATGACCATCAACGCCGCTAAGGGTTTACAAGATGTACTCAAGTCGAATCTCGGTCCTAAGGGAACTATCAAGATGCTTGTTGGTGGTTCCGGAGATATCAAGCTTACCAAGGATGGCAACACGCTTTTGAAGGAAATGCAAATTCAAAATCCAACAGCCATCATGATTGCCAGGACAGCTGTTGCTCAGGATGACATAAGTGGTGATGGTACTACTTCTACTGTCATCTTCATTGGTGAGCTTATGAAACAGTCGGAGCGTTGCATCGATGAAGGAATGCATCCACGTGTCTTAGTTGATGGTTTCGAGATTGCAAAAAGAGCCACTCTCCAGTTTCTCGACACTTTCAAGACACCTGTGGTTATGGGTGATGAGCCTGATAAAGAGATTCTGAAAATGGTTGCCAGGACAACGCTAAGAACTAAGCTGTATGAAGGCTTAGCTGATCAACTGACTGATATTGTTGTTAATTCGGTTCTCTGCATTCGGAAGCCTCAGGAACCTATTGATCTGTTTATGGTTGAGATTATGCACATGCGCCATAAATTTGATGTTGACACACGATTGGTTGAGGGGCTTGTTCTTGATCATGGTTCGAGACACCCTGACATGAAGCGACGTGCGGAGAATTGTCACATCCTGACTTGCAATGTGTCATTAGAGTACGAGAAGAGTGAAATTAATGCAGGATTCTTCTACTCGAATGCGGAGCAGAGGGAAGCCATGGTTACTGCTGAGCGACGTTCCGTTGATGAAAGAGTTCAGAAAATTATTGAGCTGAAGAACAAGGTTTGTGCTGGTAATGATAACAGCTTTGTTATCCTAAATCAAAAGGGTATTGATCCCCCGTCATTGGATCTTCTTGCTAGAGAAGGGATAATCGCCCTTAGAAGGGCAAAGAGGAGGAACATGGAACGTTTGGTTTTGGCATGTGGTGGAGAAGCTGTGAACTCTGTCGATGACTTGACCCCTGATTGTCTTGGTTGGGCTGGACTTGTATATGAGCACGTTCTTGGAGAGGAGAAGTACACATTTGTTGAGCAAGTGAAGAATCCTCATTCATGTACTATCCTCATAAAAGGGCCTAATGACCACACTATTGCTCAAATTAAGGACGCTGTCCGTGATGGTTTAAGATCAGTTAAGAATACCCTAGAAGATGAGTGTGTCGTATTAGGAGCTGGAGCTTTTGAAGTTGCAGCAAGACAACACTTAATCAATGAAGTCAAGAAAACCGTTCAAGGGCGCGCTCAACTCGGTGTTGAAGCTTTTGCTAATGCCCTTCTCGTAGTGCCTAAGACGCTTGCTGAAAATGCTGGGCTTGACACTCAAGACGTTATCATTTCTCTAACGAGTGAGCATGACAAAGGAAACATCGTGGGACTAGACCTACAGGATGGAGAACCCGTCGACCCTCAGCTTGCCGGCATTTTCGACAATTACTCGGTGAAACGCCAACTTATCAATTCAGGGCCGGTGATTGCTTCGCAGTTGCTATTAGTGGATGAAGTGATTCGTGCAGGAAGAAATATGAGGAAGCCTACTTAA

Protein Analysis

535

Amino Acids

58.95

Weight (kDa)

5.83

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 29 - 528 2.6e-168 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1470
AccII CGCG 2 cut(s) 53, 1308
AccIII TCCGGA 1 cut(s) 149
AciI CCGC 4 cut(s) 51, 75, 683, 767
AclI AACGTT 1 cut(s) 976
AclWI GGATC 2 cut(s) 896, 924
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 2 cut(s) 201, 605
AcuI CTGAAG 1 cut(s) 860
AcvI CACGTG 1 cut(s) 347
AfaI GTAC 6 cut(s) 15, 96, 271, 725, 1088, 1126
AfiI CCNNNNNNNGG 4 cut(s) 913, 951, 952, 1302
AflIII ACRYGT 1 cut(s) 346
AgsI TTSAA 6 cut(s) 190, 206, 409, 1253, 1301, 1325
AjiI CACGTC 1 cut(s) 680
AjnI CCWGG 2 cut(s) 230, 464
Alw21I GWGCWC 2 cut(s) 39, 1068
Alw26I GTCTC 1 cut(s) 652
AlwI GGATC 2 cut(s) 896, 924
Aor13HI TCCGGA 1 cut(s) 149
AoxI GGCC 3 cut(s) 48, 1142, 1532
ApeKI GCWGC 1 cut(s) 1259
ApoI RAATTY 2 cut(s) 201, 605
AseI ATTAAT 1 cut(s) 741
Asp700I GAANNNNTTC 2 cut(s) 819, 1572
AspLEI GCGC 4 cut(s) 55, 600, 1308, 1310
AspS9I GGNCC 3 cut(s) 113, 1142, 1532
AsuHPI GGTGA 5 cut(s) 275, 308, 440, 1519, 1549
AvaII GGWCC 1 cut(s) 113
AxyI CCTNAGG 2 cut(s) 117, 555
BbrPI CACGTG 1 cut(s) 347
Bbv12I GWGCWC 2 cut(s) 39, 1068
BbvCI CCTCAGC 2 cut(s) 30, 1476
BbvI GCAGC 1 cut(s) 1271
BccI CCATC 7 cut(s) 74, 167, 227, 260, 353, 1181, 1451
BciT130I CCWGG 2 cut(s) 232, 466
BclI TGATCA 2 cut(s) 505, 646
BcoDI GTCTC 1 cut(s) 652
BfaI CTAG 3 cut(s) 927, 1214, 1445
BfmI CTRYAG 1 cut(s) 1451
BisI GCNGC 3 cut(s) 51, 75, 1260
BlpI GCTNAGC 2 cut(s) 499, 795
BlsI GCNGC 3 cut(s) 52, 76, 1261
Bme1390I CCNGG 2 cut(s) 232, 466
Bme18I GGWCC 1 cut(s) 113
BmgBI CACGTC 1 cut(s) 680
BmgT120I GGNCC 3 cut(s) 113, 1142, 1532
BmiI GGNNCC 2 cut(s) 148, 561
BmrFI CCNGG 2 cut(s) 232, 466
BmsI GCATC 3 cut(s) 123, 333, 349
BplI GAGNNNNNCTC 2 cut(s) 372, 404
BpmI CTGGAG 2 cut(s) 374, 1263
Bpu10I CCTNAGC 4 cut(s) 30, 78, 246, 1476
Bpu1102I GCTNAGC 2 cut(s) 499, 795
BpuEI CTTGAG 2 cut(s) 83, 1374
Bsa29I ATCGAT 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 347
BsaJI CCNNGG 2 cut(s) 168, 783
BsaWI WCCGGW 1 cut(s) 149
BsaXI ACNNNNNCTCC 4 cut(s) 990, 1020, 1452, 1482
Bsc4I CCNNNNNNNGG 4 cut(s) 913, 951, 952, 1302
Bse118I RCCGGY 2 cut(s) 1484, 1534
Bse1I ACTGG 1 cut(s) 391
Bse21I CCTNAGG 2 cut(s) 117, 555
Bse3DI GCAATG 1 cut(s) 715
BseAI TCCGGA 1 cut(s) 149
BseBI CCWGG 2 cut(s) 232, 466
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 2 cut(s) 168, 783
BseGI GGATG 6 cut(s) 178, 256, 340, 696, 1462, 1570
BseLI CCNNNNNNNGG 4 cut(s) 913, 951, 952, 1302
BseMI GCAATG 1 cut(s) 715
BseMII CTCAG 5 cut(s) 21, 260, 569, 786, 1490
BseNI ACTGG 1 cut(s) 391
BsePI GCGCGC 1 cut(s) 1306
BseRI GAGGAG 2 cut(s) 976, 1094
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 1271
BseYI CCCAGC 1 cut(s) 1376
BsgI GTGCAG 1 cut(s) 1599
Bsh1236I CGCG 2 cut(s) 53, 1308
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 3 cut(s) 50, 1144, 1534
BshVI ATCGAT 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 2 cut(s) 39, 1068
BsiSI CCGG 3 cut(s) 150, 1485, 1535
BslFI GGGAC 1 cut(s) 1455
BslI CCNNNNNNNGG 4 cut(s) 913, 951, 952, 1302
BsmAI GTCTC 1 cut(s) 652
BsmFI GGGAC 1 cut(s) 1455
BsmI GAATGC 3 cut(s) 342, 543, 769
BsnI GGCC 3 cut(s) 50, 1144, 1534
Bsp1286I GDGCHC 2 cut(s) 39, 1068
Bsp13I TCCGGA 1 cut(s) 149
Bsp143I GATC 6 cut(s) 505, 568, 646, 901, 916, 1196
Bsp1720I GCTNAGC 2 cut(s) 499, 795
Bsp19I CCATGG 1 cut(s) 783
BspACI CCGC 4 cut(s) 51, 75, 683, 767
BspANI GGCC 3 cut(s) 50, 1144, 1534
BspCNI CTCAG 5 cut(s) 22, 259, 568, 787, 1489
BspDI ATCGAT 1 cut(s) 327
BspEI TCCGGA 1 cut(s) 149
BspFNI CGCG 2 cut(s) 53, 1308
BspHI TCATGA 1 cut(s) 222
BspLI GGNNCC 2 cut(s) 148, 561
BspPI GGATC 2 cut(s) 896, 924
BsrDI GCAATG 1 cut(s) 715
BsrFI RCCGGY 2 cut(s) 1484, 1534
BsrI ACTGG 1 cut(s) 391
BssAI RCCGGY 2 cut(s) 1484, 1534
BssECI CCNNGG 2 cut(s) 168, 783
BssHII GCGCGC 1 cut(s) 1306
BssMI GATC 6 cut(s) 505, 568, 646, 901, 916, 1196
BssT1I CCWWGG 2 cut(s) 168, 783
Bst2UI CCWGG 2 cut(s) 232, 466
Bst4CI ACNGT 3 cut(s) 283, 312, 1297
Bst6I CTCTTC 1 cut(s) 725
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 4 cut(s) 1308, 1365, 1482, 1486
BstDSI CCRYGG 1 cut(s) 783
BstF5I GGATG 6 cut(s) 178, 256, 340, 696, 1462, 1570
BstFNI CGCG 2 cut(s) 53, 1308
BstHHI GCGC 4 cut(s) 55, 600, 1308, 1310
BstKTI GATC 6 cut(s) 508, 571, 649, 904, 919, 1199
BstMAI GTCTC 1 cut(s) 652
BstMBI GATC 6 cut(s) 505, 568, 646, 901, 916, 1196
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 4 cut(s) 236, 380, 1360, 1373
BstNI CCWGG 2 cut(s) 232, 466
BstNSI RCATGY 2 cut(s) 598, 993
BstSCI CCNGG 2 cut(s) 230, 464
BstSFI CTRYAG 1 cut(s) 1451
BstUI CGCG 2 cut(s) 53, 1308
BstV1I GCAGC 1 cut(s) 1271
BstX2I RGATCY 1 cut(s) 916
BstYI RGATCY 1 cut(s) 916
BstZI CGGCCG 1 cut(s) 48
Bsu15I ATCGAT 1 cut(s) 327
Bsu36I CCTNAGG 2 cut(s) 117, 555
BsuRI GGCC 3 cut(s) 50, 1144, 1534
BsuTUI ATCGAT 1 cut(s) 327
BtgI CCRYGG 1 cut(s) 783
BtrI CACGTC 1 cut(s) 680
BtsCI GGATG 6 cut(s) 178, 256, 340, 696, 1462, 1570
Cac8I GCNNGC 4 cut(s) 1308, 1365, 1482, 1486
CciI TCATGA 1 cut(s) 222
CfoI GCGC 4 cut(s) 55, 600, 1308, 1310
Cfr10I RCCGGY 2 cut(s) 1484, 1534
Cfr13I GGNCC 3 cut(s) 113, 1142, 1532
ClaI ATCGAT 1 cut(s) 327
CseI GACGC 2 cut(s) 1184, 1369
Csp6I GTAC 6 cut(s) 14, 95, 270, 724, 1087, 1125
CviAII CATG 9 cut(s) 223, 595, 650, 670, 784, 970, 990, 1122, 1421
CviQI GTAC 6 cut(s) 14, 95, 270, 724, 1087, 1125
DpnI GATC 6 cut(s) 507, 570, 648, 903, 918, 1198
DpnII GATC 6 cut(s) 505, 568, 646, 901, 916, 1196
EaeI YGGCCR 1 cut(s) 48
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 725
EarI CTCTTC 1 cut(s) 725
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 2 cut(s) 168, 783
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 113
Eco52I CGGCCG 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 860
Eco72I CACGTG 1 cut(s) 347
Eco81I CCTNAGG 2 cut(s) 117, 555
EcoO109I RGGNCCY 1 cut(s) 1142
EcoRII CCWGG 2 cut(s) 230, 464
EcoRV GATATC 1 cut(s) 157
EcoT14I CCWWGG 2 cut(s) 168, 783
EcoT22I ATGCAT 1 cut(s) 342
ErhI CCWWGG 2 cut(s) 168, 783
FaeI CATG 9 cut(s) 226, 598, 653, 673, 787, 973, 993, 1125, 1424
FaqI GGGAC 1 cut(s) 1455
FatI CATG 9 cut(s) 222, 594, 649, 669, 783, 969, 989, 1121, 1420
FauNDI CATATG 1 cut(s) 60
FbaI TGATCA 2 cut(s) 505, 646
FblI GTMKAC 1 cut(s) 1470
Fnu4HI GCNGC 3 cut(s) 51, 75, 1260
FokI GGATG 6 cut(s) 185, 263, 327, 683, 1469, 1577
Fsp4HI GCNGC 3 cut(s) 51, 75, 1260
FspBI CTAG 3 cut(s) 927, 1214, 1445
GlaI GCGC 4 cut(s) 54, 599, 1307, 1309
GluI GCNGC 3 cut(s) 51, 75, 1260
GsaI CCCAGC 1 cut(s) 1380
GsuI CTGGAG 2 cut(s) 374, 1263
HaeIII GGCC 3 cut(s) 50, 1144, 1534
HapII CCGG 3 cut(s) 150, 1485, 1535
HgaI GACGC 2 cut(s) 1184, 1369
HhaI GCGC 4 cut(s) 55, 600, 1308, 1310
Hin1II CATG 9 cut(s) 226, 598, 653, 673, 787, 973, 993, 1125, 1424
Hin6I GCGC 4 cut(s) 53, 598, 1306, 1308
HinP1I GCGC 4 cut(s) 53, 598, 1306, 1308
HincII GTYRAC 2 cut(s) 616, 1471
HindII GTYRAC 2 cut(s) 616, 1471
HindIII AAGCTT 2 cut(s) 161, 1326
HinfI GANTC 6 cut(s) 19, 106, 448, 750, 1111, 1573
HpaII CCGG 3 cut(s) 150, 1485, 1535
HphI GGTGA 5 cut(s) 275, 308, 440, 1519, 1549
Hpy166II GTNNAC 6 cut(s) 14, 86, 616, 1009, 1089, 1471
Hpy188I TCNGA 4 cut(s) 316, 453, 549, 825
Hpy8I GTNNAC 6 cut(s) 14, 86, 616, 1009, 1089, 1471
Hpy99I CGWCG 3 cut(s) 681, 804, 1472
HpyAV CCTTC 6 cut(s) 184, 326, 488, 926, 945, 1352
HpyCH4III ACNGT 3 cut(s) 283, 312, 1297
HpyCH4IV ACGT 6 cut(s) 346, 679, 802, 976, 1068, 1395
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 380, 1360, 1373
HpySE526I ACGT 6 cut(s) 346, 679, 802, 976, 1068, 1395
Hsp92II CATG 9 cut(s) 226, 598, 653, 673, 787, 973, 993, 1125, 1424
HspAI GCGC 4 cut(s) 53, 598, 1306, 1308
Kpn2I TCCGGA 1 cut(s) 149
KroI GCCGGC 1 cut(s) 1484
KroNI GCCGGC 1 cut(s) 1486
Ksp22I TGATCA 2 cut(s) 505, 646
Kzo9I GATC 6 cut(s) 505, 568, 646, 901, 916, 1196
LmnI GCTCC 4 cut(s) 316, 769, 1238, 1244
Lsp1109I GCAGC 1 cut(s) 1271
LweI GCATC 3 cut(s) 123, 333, 349
MaeI CTAG 3 cut(s) 927, 1214, 1445
MaeII ACGT 6 cut(s) 346, 679, 802, 976, 1068, 1395
MaeIII GTNAC 2 cut(s) 692, 787
MalI GATC 6 cut(s) 507, 570, 648, 903, 918, 1198
MboI GATC 6 cut(s) 505, 568, 646, 901, 916, 1196
MboII GAAGA 8 cut(s) 280, 742, 745, 853, 911, 1120, 1229, 1596
MflI RGATCY 1 cut(s) 916
MhlI GDGCHC 2 cut(s) 39, 1068
MluCI AATT 9 cut(s) 201, 529, 605, 688, 738, 828, 1167, 1498, 1525
MmeI TCCRAC 2 cut(s) 236, 294
Mph1103I ATGCAT 1 cut(s) 342
MroI TCCGGA 1 cut(s) 149
MroNI GCCGGC 1 cut(s) 1484
MroXI GAANNNNTTC 2 cut(s) 819, 1572
MseI TTAA 7 cut(s) 528, 741, 1170, 1193, 1203, 1274, 1606
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 3 cut(s) 150, 1485, 1535
MspR9I CCNGG 2 cut(s) 232, 466
Mva1269I GAATGC 3 cut(s) 342, 543, 769
MvaI CCWGG 2 cut(s) 232, 466
MvnI CGCG 2 cut(s) 53, 1308
MwoI GCNNNNNNNGC 4 cut(s) 236, 380, 1360, 1373
NaeI GCCGGC 1 cut(s) 1486
NcoI CCATGG 1 cut(s) 783
NdeI CATATG 1 cut(s) 60
NdeII GATC 6 cut(s) 505, 568, 646, 901, 916, 1196
NgoMIV GCCGGC 1 cut(s) 1484
NlaIII CATG 9 cut(s) 226, 598, 653, 673, 787, 973, 993, 1125, 1424
NlaIV GGNNCC 2 cut(s) 148, 561
NmuCI GTSAC 1 cut(s) 692
NsiI ATGCAT 1 cut(s) 342
NspI RCATGY 2 cut(s) 598, 993
PagI TCATGA 1 cut(s) 222
PauI GCGCGC 1 cut(s) 1306
PctI GAATGC 3 cut(s) 342, 543, 769
PdiI GCCGGC 1 cut(s) 1486
PdmI GAANNNNTTC 2 cut(s) 819, 1572
PfeI GAWTC 6 cut(s) 19, 106, 448, 750, 1111, 1573
PflFI GACNNNGTC 1 cut(s) 1178
PkrI GCNGC 3 cut(s) 52, 76, 1261
PmaCI CACGTG 1 cut(s) 347
PmlI CACGTG 1 cut(s) 347
Ppu21I YACGTR 1 cut(s) 347
PshBI ATTAAT 1 cut(s) 741
Psp1406I AACGTT 1 cut(s) 976
Psp6I CCWGG 2 cut(s) 230, 464
PspCI CACGTG 1 cut(s) 347
PspFI CCCAGC 1 cut(s) 1376
PspGI CCWGG 2 cut(s) 230, 464
PspN4I GGNNCC 2 cut(s) 148, 561
PspPI GGNCC 3 cut(s) 113, 1142, 1532
PsuI RGATCY 1 cut(s) 916
PsyI GACNNNGTC 1 cut(s) 1178
PteI GCGCGC 1 cut(s) 1306
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 6 cut(s) 15, 96, 271, 725, 1088, 1126
RsaNI GTAC 6 cut(s) 14, 95, 270, 724, 1087, 1125
RseI CAYNNNNRTG 1 cut(s) 345
SalI GTCGAC 1 cut(s) 1469
SaqAI TTAA 7 cut(s) 528, 741, 1170, 1193, 1203, 1274, 1606
SatI GCNGC 3 cut(s) 51, 75, 1260
Sau3AI GATC 6 cut(s) 505, 568, 646, 901, 916, 1196
Sau96I GGNCC 3 cut(s) 113, 1142, 1532
ScrFI CCNGG 2 cut(s) 232, 466
SduI GDGCHC 2 cut(s) 39, 1068
SfaNI GCATC 3 cut(s) 123, 333, 349
SfcI CTRYAG 1 cut(s) 1451
SinI GGWCC 1 cut(s) 113
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 2 cut(s) 98, 1389
SmoI CTYRAG 2 cut(s) 98, 1389
Sse9I AATT 9 cut(s) 201, 529, 605, 688, 738, 828, 1167, 1498, 1525
SsiI CCGC 4 cut(s) 51, 75, 683, 767
SspMI CTAG 3 cut(s) 927, 1214, 1445
StyD4I CCNGG 2 cut(s) 230, 464
StyI CCWWGG 2 cut(s) 168, 783
TaaI ACNGT 3 cut(s) 283, 312, 1297
TaiI ACGT 6 cut(s) 349, 682, 805, 979, 1071, 1398
TaqI TCGA 9 cut(s) 104, 327, 366, 399, 656, 761, 1017, 1470, 1494
TaqII GACCGA 1 cut(s) 101
TasI AATT 9 cut(s) 201, 529, 605, 688, 738, 828, 1167, 1498, 1525
TatI WGTACW 4 cut(s) 13, 94, 1086, 1124
TauI GCSGC 2 cut(s) 53, 77
TfiI GAWTC 6 cut(s) 19, 106, 448, 750, 1111, 1573
Tru1I TTAA 7 cut(s) 528, 741, 1170, 1193, 1203, 1274, 1606
Tru9I TTAA 7 cut(s) 528, 741, 1170, 1193, 1203, 1274, 1606
TseFI GTSAC 1 cut(s) 692
TseI GCWGC 1 cut(s) 1259
Tsp45I GTSAC 1 cut(s) 692
TspGWI ACGGA 2 cut(s) 796, 1172
Tth111I GACNNNGTC 1 cut(s) 1178
VpaK11BI GGWCC 1 cut(s) 113
VspI ATTAAT 1 cut(s) 741
XapI RAATTY 2 cut(s) 201, 605
XceI RCATGY 2 cut(s) 598, 993
XmiI GTMKAC 1 cut(s) 1470
XmnI GAANNNNTTC 2 cut(s) 819, 1572
XspI CTAG 3 cut(s) 927, 1214, 1445
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.