Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0003345

Physical Location & Seq

Genomic Coordinates Forward (+)

8902594 .. 8907194

Gene Structure 4601 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1104900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGTCGATTCGAGTGCTGAATCCGAATGCGGAGGTGCTGAACAAATCGGCGGCGCTGCACATGAACATCAACGCCGCCAAGGGCTTGCAGGACGTCCTCAAGACCAACCTCGGCCCCAAGGGCACCATCAAAATGCTTGTTGGTGGAGCTGGCGACATTAAGCTCACTAAGGATGGCAACACTCTGCTCAAAGAAATGCAAATTCAGAACCCAACAGCAATTATGATTGCAAGGACCGCTGTTGCCCAAGATGACATAAGCGGAGATGGCACCACGTCCACTGTCTTATTCATCGGCGAGCTTATGAAACAATCAGAGCGATACATTGACGAAGGGATGCATCCCCGTGTCCTAGTTGATGGTTTTGAGATTGCCAAAAGAGCAACGCTTCAGTTTCTTGAGAAATTCAAAACTCCTGTTGTGATGGGCAATGAGCCCGACAAAGAGATTCTGAAAATGGTAGCAAGGACAACATTGCGAACAAAGTTATATGAAGCATTGGCGGATCAATTGACCGACATAGTTGTTAATTCGGTTCTTTGCATCCGCAAACCTGAGGAACCCATTGATCTGTTTATGGTGGAGATTATGCACATGCGACACAAATTTGATGTAGACACACGTTTGGTCGAGGGTCTTGTTCTGGATCATGGTTCTAGGCATCCTGATATGAAGCGGAGAGCAGAGAATTGTTACATCTTGACAAGCAATGTATCTTTGGAGTACGATAAAAGTGAAGTAAACTCAGGCTTTTTCTATTCAAATGCGGAGCAGAGAGAAGCAATGGTTTTAGCTGAAAGGCGTCAGGTTGATGAAAGAGTTAAAAAAATCATTGAACTGAAAAATAAGGTTTGCTCTGGTAATAATAATAATTTTGTCGTTATCAATCAGAAGGGAATTGATCCCCCATCTCTGGATCTCCTTGCAAGGGCAGGGATTATTGCCTTGAGAAGAGCAAAGAGGAGAAATATGGAACGGTTGGTTTTGGCTTGTGGCGGAGAGGCTGTGAACTCCGTAGATGATCTAACTCCTGATTGCCTTGGTTGGGCTGGACTTGTATACGAGCATGTCCTTGGTGAAGAGAAGTATACATTTGTCGAAAATGTTAAAAACCCCCATTCTTGCACAATCTTGATCAAAGGACCTAATGACCATACAATTGCTCAGATTAAGGATGCTGTTCGTGATGGTCTGAGGGCAGTTAAAAACACAATTGAAGATGAAGCTGTCATCTTAGGTGCTGGAGCTTTTGAAGTTGCAGCCAGACAATATCTAGTAAATGAAGTGAAGAAAACTGTTAAAGGGCGCGCTCAACTAGGCGTTCAAGCTTTTGCTGATGCTCTCCTTGTGGTACCCAAGACACTTGCTGAGAATTCTGGCCTCGATACGCAAGATGTGATAATTGCCCTTACGGGAGAGCATGATCAGGGAAATGTCGTGGGACTAAATCACAACACTGGAGAACCGCTTGACCCACAGATGGAGGGTATCTTTGACAACTACTCTGTGAAGCGACAGATTATAAACTCAGGGCCCGTAATTGCATCTCAACTGTTGTTGGTCGATGAAGTAATTCGTGCTGGGCGTAACATGCGGAAGCCAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005832 GO:0006457 GO:0006458 GO:0008150 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0032991 GO:0042221 GO:0044183 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0046686 GO:0050896 GO:0051082 GO:0061077 GO:0101031

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cpn60_TCP1

Protein Analysis

536

Amino Acids

59.12

Weight (kDa)

6.31

Isoelectric Point (pI)

32.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	29 - 528	1.1e-167	TCP-1/cpn60 chaperonin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003345)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02530 AT5G16070
fragaria_vesca	FvH4_1g11210
malus_domestica	MD09G1086000.v1.1 MD17G1104900.v1.1
prunus_persica	Prupe.3G238800_v2.0.a1
pyrus_communis	pycom09g01130 pycom17g07580
rosa_chinensis	RchiOBHm_Chr2g0098621 RchiOBHm_Chr2g0138531 RchiOBHm_Chr4g0407281 RchiOBHm_Chr4g0409191 RchiOBHm_Chr6g0270681
rosa_laevigata	RLG00000013776 RLG00000016815
rosa_multiflora	Rmu_co8186612.1_g000001 Rmu_sc0000474.1_g000003 Rmu_sc0002106.1_g000012 Rmu_sc0002776.1_g000012
rosa_roxburghii	Rroxscaffold_2G00143840 Rroxscaffold_7G00197690
rosa_rugosa	Rorug02G0073800 Rorug06G0055700
rosa_samantha	Rh2AG121400 Rh2BG125000 Rh2CG126000 Rh2DG127100 Rh5BG537700 Rh6AG175000 Rh6AG275700 Rh6CG174300 Rh6DG166700 Rh7CG256600
rosa_wichuraiana	Rw0G004140 Rw0G012590 Rw6G014910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1523
AatII	GACGTC	1 cut(s)	96
Acc65I	GGTACC	1 cut(s)	1351
AccB1I	GGYRCC	3 cut(s)	122, 269, 1351
AccI	GTMKAC	3 cut(s)	615, 1059, 1088
AccII	CGCG	1 cut(s)	1308
AclWI	GGATC	4 cut(s)	513, 654, 896, 924
AcsI	RAATTY	4 cut(s)	201, 404, 605, 1372
AcuI	CTGAAG	1 cut(s)	374
AcyI	GRCGYC	2 cut(s)	93, 802
AfaI	GTAC	2 cut(s)	725, 1353
AfiI	CCNNNNNNNGG	4 cut(s)	913, 928, 1045, 1480
AflIII	ACRYGT	1 cut(s)	620
AgsI	TTSAA	6 cut(s)	409, 762, 836, 1217, 1253, 1325
AjiI	CACGTC	1 cut(s)	276
AluBI	AGCT	7 cut(s)	149, 163, 301, 794, 1226, 1247, 1328
AluI	AGCT	7 cut(s)	149, 163, 301, 794, 1226, 1247, 1328
AlwI	GGATC	4 cut(s)	513, 654, 896, 924
AoxI	GGCC	3 cut(s)	112, 1378, 1532
ApaI	GGGCCC	1 cut(s)	1536
ApeKI	GCWGC	2 cut(s)	55, 1259
ApoI	RAATTY	4 cut(s)	201, 404, 605, 1372
Asp700I	GAANNNNTTC	1 cut(s)	1572
Asp718I	GGTACC	1 cut(s)	1351
AspLEI	GCGC	3 cut(s)	55, 1308, 1310
AspS9I	GGNCC	5 cut(s)	113, 234, 1142, 1532, 1533
AsuHPI	GGTGA	1 cut(s)	1088
AvaII	GGWCC	2 cut(s)	234, 1142
AxyI	CCTNAGG	1 cut(s)	555
BaeGI	GKGCMC	2 cut(s)	125, 1536
BanI	GGYRCC	3 cut(s)	122, 269, 1351
BanII	GRGCYC	2 cut(s)	438, 1536
BbvI	GCAGC	2 cut(s)	42, 1271
BccI	CCATC	8 cut(s)	134, 167, 260, 353, 418, 916, 1181, 1474
BcgI	CGANNNNNNTGC	1 cut(s)	29
BclI	TGATCA	2 cut(s)	1134, 1423
BfaI	CTAG	4 cut(s)	353, 657, 1274, 1316
BfoI	RGCGCY	1 cut(s)	56
BglI	GCCNNNNNGGC	1 cut(s)	120
BisI	GCNGC	4 cut(s)	51, 56, 75, 1260
BlsI	GCNGC	4 cut(s)	52, 57, 76, 1261
Bme18I	GGWCC	2 cut(s)	234, 1142
BmgBI	CACGTC	1 cut(s)	276
BmgT120I	GGNCC	5 cut(s)	113, 234, 1142, 1532, 1533
BmiI	GGNNCC	6 cut(s)	115, 124, 271, 561, 1353, 1534
BmsI	GCATC	7 cut(s)	327, 349, 552, 670, 1165, 1327, 1553
BpmI	CTGGAG	2 cut(s)	1263, 1479
BpuEI	CTTGAG	3 cut(s)	83, 419, 967
BsaHI	GRCGYC	2 cut(s)	93, 802
BsaJI	CCNNGG	5 cut(s)	78, 109, 117, 1039, 1072
BsaXI	ACNNNNNCTCC	2 cut(s)	990, 1020
Bsc4I	CCNNNNNNNGG	4 cut(s)	913, 928, 1045, 1480
Bse1I	ACTGG	1 cut(s)	1462
Bse21I	CCTNAGG	1 cut(s)	555
Bse3DI	GCAATG	4 cut(s)	436, 473, 715, 789
BseDI	CCNNGG	5 cut(s)	78, 109, 117, 1039, 1072
BseGI	GGATG	6 cut(s)	178, 340, 342, 543, 661, 1180
BseLI	CCNNNNNNNGG	4 cut(s)	913, 928, 1045, 1480
BseMI	GCAATG	4 cut(s)	436, 473, 715, 789
BseMII	CTCAG	6 cut(s)	546, 759, 1178, 1184, 1359, 1542
BseNI	ACTGG	1 cut(s)	1462
BsePI	GCGCGC	1 cut(s)	1306
BseRI	GAGGAG	1 cut(s)	976
BseSI	GKGCMC	2 cut(s)	125, 1536
BseXI	GCAGC	2 cut(s)	42, 1271
BseYI	CCCAGC	1 cut(s)	1580
BsgI	GTGCAG	1 cut(s)	41
Bsh1236I	CGCG	1 cut(s)	1308
BshFI	GGCC	3 cut(s)	114, 1380, 1534
BshNI	GGYRCC	3 cut(s)	122, 269, 1351
BslFI	GGGAC	1 cut(s)	1455
BslI	CCNNNNNNNGG	4 cut(s)	913, 928, 1045, 1480
BsmFI	GGGAC	1 cut(s)	1455
BsmI	GAATGC	1 cut(s)	31
BsnI	GGCC	3 cut(s)	114, 1380, 1534
Bsp120I	GGGCCC	1 cut(s)	1532
Bsp1286I	GDGCHC	3 cut(s)	125, 438, 1536
Bsp143I	GATC	8 cut(s)	505, 568, 646, 901, 916, 1021, 1134, 1423
BspANI	GGCC	3 cut(s)	114, 1380, 1534
BspCNI	CTCAG	6 cut(s)	547, 758, 1177, 1185, 1360, 1541
BspFNI	CGCG	1 cut(s)	1308
BspLI	GGNNCC	6 cut(s)	115, 124, 271, 561, 1353, 1534
BspPI	GGATC	4 cut(s)	513, 654, 896, 924
BspQI	GCTCTTC	1 cut(s)	946
BspT107I	GGYRCC	3 cut(s)	122, 269, 1351
BsrDI	GCAATG	4 cut(s)	436, 473, 715, 789
BsrI	ACTGG	1 cut(s)	1462
BssECI	CCNNGG	5 cut(s)	78, 109, 117, 1039, 1072
BssHII	GCGCGC	1 cut(s)	1306
BssMI	GATC	8 cut(s)	505, 568, 646, 901, 916, 1021, 1134, 1423
BssNAI	GTATAC	2 cut(s)	1060, 1089
BssNI	GRCGYC	2 cut(s)	93, 802
BssT1I	CCWWGG	4 cut(s)	78, 117, 1039, 1072
Bst1107I	GTATAC	2 cut(s)	1060, 1089
Bst4CI	ACNGT	4 cut(s)	283, 978, 1297, 1554
Bst6I	CTCTTC	2 cut(s)	946, 1074
BstACI	GRCGYC	2 cut(s)	93, 802
BstC8I	GCNNGC	4 cut(s)	86, 151, 299, 1308
BstDEI	CTNAG	8 cut(s)	168, 555, 745, 1164, 1193, 1234, 1368, 1528
BstF5I	GGATG	6 cut(s)	178, 340, 342, 543, 661, 1180
BstFNI	CGCG	1 cut(s)	1308
BstH2I	RGCGCY	1 cut(s)	56
BstHHI	GCGC	3 cut(s)	55, 1308, 1310
BstKTI	GATC	8 cut(s)	508, 571, 649, 904, 919, 1024, 1137, 1426
BstMBI	GATC	8 cut(s)	505, 568, 646, 901, 916, 1021, 1134, 1423
BstMWI	GCNNNNNNNGC	5 cut(s)	120, 236, 267, 380, 1591
BstNSI	RCATGY	3 cut(s)	598, 1070, 1594
BstSLI	GKGCMC	2 cut(s)	125, 1536
BstUI	CGCG	1 cut(s)	1308
BstV1I	GCAGC	2 cut(s)	42, 1271
BstX2I	RGATCY	1 cut(s)	916
BstYI	RGATCY	1 cut(s)	916
BstZ17I	GTATAC	2 cut(s)	1060, 1089
Bsu36I	CCTNAGG	1 cut(s)	555
BsuRI	GGCC	3 cut(s)	114, 1380, 1534
BtrI	CACGTC	1 cut(s)	276
BtsCI	GGATG	6 cut(s)	178, 340, 342, 543, 661, 1180
BtsIMutI	CAGTG	2 cut(s)	279, 1455
Cac8I	GCNNGC	4 cut(s)	86, 151, 299, 1308
CfoI	GCGC	3 cut(s)	55, 1308, 1310
Cfr13I	GGNCC	5 cut(s)	113, 234, 1142, 1532, 1533
CseI	GACGC	1 cut(s)	791
Csp6I	GTAC	2 cut(s)	724, 1352
CviAII	CATG	6 cut(s)	61, 595, 650, 1067, 1421, 1591
CviQI	GTAC	2 cut(s)	724, 1352
DdeI	CTNAG	8 cut(s)	168, 555, 745, 1164, 1193, 1234, 1368, 1528
DpnI	GATC	8 cut(s)	507, 570, 648, 903, 918, 1023, 1136, 1425
DpnII	GATC	8 cut(s)	505, 568, 646, 901, 916, 1021, 1134, 1423
Eam1104I	CTCTTC	2 cut(s)	946, 1074
EarI	CTCTTC	2 cut(s)	946, 1074
EciI	GGCGGA	2 cut(s)	518, 1011
Eco130I	CCWWGG	4 cut(s)	78, 117, 1039, 1072
Eco24I	GRGCYC	2 cut(s)	438, 1536
Eco47I	GGWCC	2 cut(s)	234, 1142
Eco57I	CTGAAG	1 cut(s)	374
Eco81I	CCTNAGG	1 cut(s)	555
EcoO109I	RGGNCCY	2 cut(s)	1142, 1532
EcoRI	GAATTC	1 cut(s)	1372
EcoT14I	CCWWGG	4 cut(s)	78, 117, 1039, 1072
EcoT22I	ATGCAT	1 cut(s)	342
EcoT38I	GRGCYC	2 cut(s)	438, 1536
ErhI	CCWWGG	4 cut(s)	78, 117, 1039, 1072
FaeI	CATG	6 cut(s)	64, 598, 653, 1070, 1424, 1594
FaqI	GGGAC	1 cut(s)	1455
FatI	CATG	6 cut(s)	60, 594, 649, 1066, 1420, 1590
FbaI	TGATCA	2 cut(s)	1134, 1423
FblI	GTMKAC	3 cut(s)	615, 1059, 1088
Fnu4HI	GCNGC	4 cut(s)	51, 56, 75, 1260
FokI	GGATG	6 cut(s)	185, 327, 349, 530, 648, 1187
FriOI	GRGCYC	2 cut(s)	438, 1536
Fsp4HI	GCNGC	4 cut(s)	51, 56, 75, 1260
FspBI	CTAG	4 cut(s)	353, 657, 1274, 1316
GlaI	GCGC	3 cut(s)	54, 1307, 1309
GluI	GCNGC	4 cut(s)	51, 56, 75, 1260
GsaI	CCCAGC	1 cut(s)	1584
GsuI	CTGGAG	2 cut(s)	1263, 1479
HaeII	RGCGCY	1 cut(s)	56
HaeIII	GGCC	3 cut(s)	114, 1380, 1534
HgaI	GACGC	1 cut(s)	791
HhaI	GCGC	3 cut(s)	55, 1308, 1310
Hin1I	GRCGYC	2 cut(s)	93, 802
Hin1II	CATG	6 cut(s)	64, 598, 653, 1070, 1424, 1594
Hin6I	GCGC	3 cut(s)	53, 1306, 1308
HinP1I	GCGC	3 cut(s)	53, 1306, 1308
HindIII	AAGCTT	1 cut(s)	1326
HinfI	GANTC	3 cut(s)	7, 19, 448
HphI	GGTGA	1 cut(s)	1088
Hpy166II	GTNNAC	6 cut(s)	279, 616, 742, 1009, 1060, 1089
Hpy188I	TCNGA	7 cut(s)	24, 207, 316, 453, 891, 1167, 1194
Hpy188III	TCNNGA	9 cut(s)	100, 398, 644, 665, 700, 914, 1031, 1132, 1184
Hpy8I	GTNNAC	6 cut(s)	279, 616, 742, 1009, 1060, 1089
HpyAV	CCTTC	2 cut(s)	326, 886
HpyCH4III	ACNGT	4 cut(s)	283, 978, 1297, 1554
HpyCH4IV	ACGT	3 cut(s)	93, 275, 622
HpyF10VI	GCNNNNNNNGC	5 cut(s)	120, 236, 267, 380, 1591
HpyF3I	CTNAG	8 cut(s)	168, 555, 745, 1164, 1193, 1234, 1368, 1528
HpySE526I	ACGT	3 cut(s)	93, 275, 622
Hsp92I	GRCGYC	2 cut(s)	93, 802
Hsp92II	CATG	6 cut(s)	64, 598, 653, 1070, 1424, 1594
HspAI	GCGC	3 cut(s)	53, 1306, 1308
KpnI	GGTACC	1 cut(s)	1355
Ksp22I	TGATCA	2 cut(s)	1134, 1423
Kzo9I	GATC	8 cut(s)	505, 568, 646, 901, 916, 1021, 1134, 1423
LguI	GCTCTTC	1 cut(s)	946
LmnI	GCTCC	3 cut(s)	146, 769, 1244
Lsp1109I	GCAGC	2 cut(s)	42, 1271
LweI	GCATC	7 cut(s)	327, 349, 552, 670, 1165, 1327, 1553
MaeI	CTAG	4 cut(s)	353, 657, 1274, 1316
MaeII	ACGT	3 cut(s)	93, 275, 622
MaeIII	GTNAC	2 cut(s)	692, 1586
MalI	GATC	8 cut(s)	507, 570, 648, 903, 918, 1023, 1136, 1425
MboI	GATC	8 cut(s)	505, 568, 646, 901, 916, 1021, 1134, 1423
MboII	GAAGA	4 cut(s)	963, 1091, 1229, 1300
MfeI	CAATTG	3 cut(s)	509, 1158, 1212
MflI	RGATCY	1 cut(s)	916
MhlI	GDGCHC	3 cut(s)	125, 438, 1536
Mph1103I	ATGCAT	1 cut(s)	342
MroXI	GAANNNNTTC	1 cut(s)	1572
MseI	TTAA	8 cut(s)	159, 528, 822, 1107, 1170, 1203, 1299, 1606
MslI	CAYNNNNRTG	2 cut(s)	131, 345
MspA1I	CMGCKG	1 cut(s)	239
MunI	CAATTG	3 cut(s)	509, 1158, 1212
Mva1269I	GAATGC	1 cut(s)	31
MvnI	CGCG	1 cut(s)	1308
MwoI	GCNNNNNNNGC	5 cut(s)	120, 236, 267, 380, 1591
NdeII	GATC	8 cut(s)	505, 568, 646, 901, 916, 1021, 1134, 1423
NlaIII	CATG	6 cut(s)	64, 598, 653, 1070, 1424, 1594
NlaIV	GGNNCC	6 cut(s)	115, 124, 271, 561, 1353, 1534
NmeAIII	GCCGAG	1 cut(s)	90
NsiI	ATGCAT	1 cut(s)	342
NspI	RCATGY	3 cut(s)	598, 1070, 1594
PauI	GCGCGC	1 cut(s)	1306
PciSI	GCTCTTC	1 cut(s)	946
PcsI	WCGNNNNNNNCGW	1 cut(s)	1582
PctI	GAATGC	1 cut(s)	31
PdmI	GAANNNNTTC	1 cut(s)	1572
PfeI	GAWTC	3 cut(s)	7, 19, 448
PkrI	GCNGC	4 cut(s)	52, 57, 76, 1261
PpuMI	RGGWCCY	1 cut(s)	1142
PsiI	TTATAA	1 cut(s)	1523
Psp5II	RGGWCCY	1 cut(s)	1142
PspFI	CCCAGC	1 cut(s)	1580
PspN4I	GGNNCC	6 cut(s)	115, 124, 271, 561, 1353, 1534
PspOMI	GGGCCC	1 cut(s)	1532
PspPI	GGNCC	5 cut(s)	113, 234, 1142, 1532, 1533
PspPPI	RGGWCCY	1 cut(s)	1142
PsuI	RGATCY	1 cut(s)	916
PteI	GCGCGC	1 cut(s)	1306
RsaI	GTAC	2 cut(s)	725, 1353
RsaNI	GTAC	2 cut(s)	724, 1352
RseI	CAYNNNNRTG	2 cut(s)	131, 345
SapI	GCTCTTC	1 cut(s)	946
SaqAI	TTAA	8 cut(s)	159, 528, 822, 1107, 1170, 1203, 1299, 1606
SatI	GCNGC	4 cut(s)	51, 56, 75, 1260
Sau3AI	GATC	8 cut(s)	505, 568, 646, 901, 916, 1021, 1134, 1423
Sau96I	GGNCC	5 cut(s)	113, 234, 1142, 1532, 1533
SduI	GDGCHC	3 cut(s)	125, 438, 1536
SfaNI	GCATC	7 cut(s)	327, 349, 552, 670, 1165, 1327, 1553
SinI	GGWCC	2 cut(s)	234, 1142
SmiMI	CAYNNNNRTG	2 cut(s)	131, 345
SmlI	CTYRAG	3 cut(s)	98, 398, 946
SmoI	CTYRAG	3 cut(s)	98, 398, 946
SspMI	CTAG	4 cut(s)	353, 657, 1274, 1316
StyI	CCWWGG	4 cut(s)	78, 117, 1039, 1072
TaaI	ACNGT	4 cut(s)	283, 978, 1297, 1554
TaiI	ACGT	3 cut(s)	96, 278, 625
TaqI	TCGA	6 cut(s)	5, 10, 630, 1098, 1383, 1563
TaqII	GACCGA	1 cut(s)	530
TauI	GCSGC	2 cut(s)	53, 77
TfiI	GAWTC	3 cut(s)	7, 19, 448
Tru1I	TTAA	8 cut(s)	159, 528, 822, 1107, 1170, 1203, 1299, 1606
Tru9I	TTAA	8 cut(s)	159, 528, 822, 1107, 1170, 1203, 1299, 1606
TscAI	CASTG	2 cut(s)	286, 1462
TseI	GCWGC	2 cut(s)	55, 1259
TspDTI	ATGAA	9 cut(s)	77, 280, 320, 507, 686, 828, 1236, 1296, 1581
TspGWI	ACGGA	1 cut(s)	1003
TspRI	CASTG	2 cut(s)	286, 1462
VpaK11BI	GGWCC	2 cut(s)	234, 1142
XapI	RAATTY	4 cut(s)	201, 404, 605, 1372
XceI	RCATGY	3 cut(s)	598, 1070, 1594
XmiI	GTMKAC	3 cut(s)	615, 1059, 1088
XmnI	GAANNNNTTC	1 cut(s)	1572
XspI	CTAG	4 cut(s)	353, 657, 1274, 1316
ZraI	GACGTC	1 cut(s)	94
Zsp2I	ATGCAT	1 cut(s)	342

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.