MD09G1086000.v1.1
TCP Family

assists the folding of proteins upon ATP hydrolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003345
Physical Location & Seq
Reverse (-)
6141011 .. 6144219
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1086000.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGTCGATTCGAGTGCTGAATCCGAATGCGGAGGTGCTGAACAAATCGGCGGCGCTGCACATGAACATCAACGCCGCCAAGGGCTTGCAGGACGTCCTCAAGACCAACCTTGGCCCCAAGGGCACCATCAAAATGCTTGTTGGCGGAGCCGGCGACATTAAGCTCACTAAGGATGGCAACACTCTGCTCAAAGAAATGCAAATTCAAAACCCAACAGCAATTATGATTGCACGGACAGCTGTTGCGCAAGATGACATTAGCGGAGATGGCACCACGTCTACTGTCTTGTTCATTGGCGAGCTTATGAAACAATCAGAACGATACATTGACGAAGGGATGCATCCCCGTGTCCTGGTTGATGGTTTTGAGATCGCCAAAAGAGCAACGCTGCAGTTTCTTGAGAAATTCAAAACTCCCGTGGTGATGGGCGATGAGCCTGACAAAGAGATTTTGAAAATGGTAGCTAGAACAACATTGCGAACAAAGTTATATGAAGCATTGGCAGATCAATTGACTGACATAGTTGTTAATTCGGTTCTTTGCATCCGCAAACCTGAGGAGCCCATTGATCTGTTCATGGTGGAGATTATGCACATGCGACACAAATTTGATGTAGACACACGCTTGGTTGAGGGTCTTGTTCTCGATCACGGTTCTAGGCATCCTGATATGAAGCGGAGAGCAGAGAATTGTTTCATCTTGACAAGCAATGTATCTTTGGAATATGACAAAAGTGAAGTAAACTCAGGCTTTTTCTATTCAAATGCGGAGCAGAGAGAAGCAATGGTTTTAGCTGAAAGGCGCCAGGTTGATGAAAGAGTTAAAAAAATCATTGACCTGAAAAATAAGGTTTGCTCTGGTAATGATAATAACTTTGTTGTTATCAATCAGAAGGGAATTGATCCCCCATCTCTGGACCTTCTTGCAAGGGCAGGGATTATTGCCCTGAGAAGAGCAAAGAGGAGAAATATGGAACGGTTGGTTTTGGCTTGTGGCGGGGAGGCTGTAAACTCCGTAGATGATTTAACTCCTGATTGCCTTGGTTGGGCTGGACTTGTATACGAGCATGTCCTTGGTGAAGAGAAGTATACATTTGTTGAAAATGTGAAGAATCCCCACTCTTGCACAATCTTAATCAAAGGTACTCCATTGGTATTTTCATCATCTGCAAAATATAAATATTCTTTGGTTATTCTACCCGGGAGTTAG

Protein Analysis

403

Amino Acids

44.76

Weight (kDa)

7.09

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 29 - 382 6.5e-115 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 96
Acc16I TGCGCA 1 cut(s) 246
AccB1I GGYRCC 3 cut(s) 122, 269, 801
AccI GTMKAC 4 cut(s) 278, 615, 1059, 1088
AciI CCGC 9 cut(s) 29, 50, 75, 144, 261, 547, 676, 767, 996
AclWI GGATC 1 cut(s) 896
AcsI RAATTY 3 cut(s) 201, 404, 605
AcyI GRCGYC 2 cut(s) 93, 802
AfaI GTAC 1 cut(s) 1144
AfiI CCNNNNNNNGG 3 cut(s) 352, 913, 1045
AgsI TTSAA 5 cut(s) 206, 409, 454, 762, 1100
AjiI CACGTC 1 cut(s) 276
AjnI CCWGG 2 cut(s) 351, 804
AluBI AGCT 5 cut(s) 163, 239, 301, 464, 794
AluI AGCT 5 cut(s) 163, 239, 301, 464, 794
AlwI GGATC 1 cut(s) 896
Ama87I CYCGRG 1 cut(s) 1199
AoxI GGCC 1 cut(s) 112
ApeKI GCWGC 2 cut(s) 55, 388
ApoI RAATTY 3 cut(s) 201, 404, 605
Asp700I GAANNNNTTC 1 cut(s) 692
AspLEI GCGC 3 cut(s) 55, 247, 804
AspS9I GGNCC 2 cut(s) 113, 916
AsuC2I CCSGG 2 cut(s) 1200, 1201
AsuHPI GGTGA 2 cut(s) 433, 1088
AvaI CYCGRG 1 cut(s) 1199
AvaII GGWCC 1 cut(s) 916
AxyI CCTNAGG 1 cut(s) 555
BaeGI GKGCMC 1 cut(s) 125
BanI GGYRCC 3 cut(s) 122, 269, 801
BanII GRGCYC 1 cut(s) 564
BbvI GCAGC 2 cut(s) 42, 375
BccI CCATC 6 cut(s) 134, 167, 260, 353, 418, 916
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 2 cut(s) 353, 806
BcnI CCSGG 2 cut(s) 1200, 1201
BfaI CTAG 2 cut(s) 465, 657
BfmI CTRYAG 1 cut(s) 389
BfoI RGCGCY 2 cut(s) 56, 805
BglI GCCNNNNNGGC 1 cut(s) 120
BisI GCNGC 4 cut(s) 51, 56, 75, 389
BlsI GCNGC 4 cut(s) 52, 57, 76, 390
Bme1390I CCNGG 4 cut(s) 353, 806, 1200, 1201
Bme18I GGWCC 1 cut(s) 916
BmeT110I CYCGRG 1 cut(s) 1199
BmgBI CACGTC 1 cut(s) 276
BmgT120I GGNCC 2 cut(s) 113, 916
BmiI GGNNCC 6 cut(s) 115, 124, 148, 271, 561, 803
BmrFI CCNGG 4 cut(s) 353, 806, 1200, 1201
BmsI GCATC 4 cut(s) 327, 349, 552, 670
BpuEI CTTGAG 2 cut(s) 83, 419
BpuMI CCSGG 2 cut(s) 1200, 1201
BsaHI GRCGYC 2 cut(s) 93, 802
BsaJI CCNNGG 7 cut(s) 78, 109, 117, 417, 1039, 1072, 1199
Bsc4I CCNNNNNNNGG 3 cut(s) 352, 913, 1045
Bse118I RCCGGY 1 cut(s) 149
Bse21I CCTNAGG 1 cut(s) 555
Bse3DI GCAATG 3 cut(s) 473, 715, 789
BseBI CCWGG 2 cut(s) 353, 806
BseDI CCNNGG 7 cut(s) 78, 109, 117, 417, 1039, 1072, 1199
BseGI GGATG 5 cut(s) 178, 340, 342, 543, 661
BseLI CCNNNNNNNGG 3 cut(s) 352, 913, 1045
BseMI GCAATG 3 cut(s) 473, 715, 789
BseMII CTCAG 3 cut(s) 546, 759, 938
BseRI GAGGAG 2 cut(s) 572, 976
BseSI GKGCMC 1 cut(s) 125
BseXI GCAGC 2 cut(s) 42, 375
BsgI GTGCAG 1 cut(s) 41
BshFI GGCC 1 cut(s) 114
BshNI GGYRCC 3 cut(s) 122, 269, 801
BsiHKCI CYCGRG 1 cut(s) 1199
BsiSI CCGG 2 cut(s) 150, 1200
BslI CCNNNNNNNGG 3 cut(s) 352, 913, 1045
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 1 cut(s) 114
BsoBI CYCGRG 1 cut(s) 1199
Bsp1286I GDGCHC 2 cut(s) 125, 564
Bsp143I GATC 5 cut(s) 369, 505, 568, 646, 901
BspACI CCGC 9 cut(s) 29, 50, 75, 144, 261, 547, 676, 767, 996
BspANI GGCC 1 cut(s) 114
BspCNI CTCAG 3 cut(s) 547, 758, 939
BspLI GGNNCC 6 cut(s) 115, 124, 148, 271, 561, 803
BspMAI CTGCAG 1 cut(s) 393
BspPI GGATC 1 cut(s) 896
BspQI GCTCTTC 1 cut(s) 946
BspT107I GGYRCC 3 cut(s) 122, 269, 801
BsrDI GCAATG 3 cut(s) 473, 715, 789
BsrFI RCCGGY 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 7 cut(s) 78, 109, 117, 417, 1039, 1072, 1199
BssMI GATC 5 cut(s) 369, 505, 568, 646, 901
BssNAI GTATAC 2 cut(s) 1060, 1089
BssNI GRCGYC 2 cut(s) 93, 802
BssT1I CCWWGG 5 cut(s) 78, 109, 117, 1039, 1072
Bst1107I GTATAC 2 cut(s) 1060, 1089
Bst2UI CCWGG 2 cut(s) 353, 806
Bst4CI ACNGT 3 cut(s) 283, 653, 978
Bst6I CTCTTC 2 cut(s) 946, 1074
BstACI GRCGYC 2 cut(s) 93, 802
BstC8I GCNNGC 3 cut(s) 86, 151, 299
BstDEI CTNAG 4 cut(s) 168, 555, 745, 947
BstDSI CCRYGG 1 cut(s) 417
BstF5I GGATG 5 cut(s) 178, 340, 342, 543, 661
BstH2I RGCGCY 2 cut(s) 56, 805
BstHHI GCGC 3 cut(s) 55, 247, 804
BstKTI GATC 5 cut(s) 372, 508, 571, 649, 904
BstMBI GATC 5 cut(s) 369, 505, 568, 646, 901
BstMWI GCNNNNNNNGC 5 cut(s) 120, 150, 236, 267, 380
BstNI CCWGG 2 cut(s) 353, 806
BstNSI RCATGY 2 cut(s) 598, 1070
BstSCI CCNGG 4 cut(s) 351, 804, 1198, 1199
BstSFI CTRYAG 1 cut(s) 389
BstSLI GKGCMC 1 cut(s) 125
BstV1I GCAGC 2 cut(s) 42, 375
BstZ17I GTATAC 2 cut(s) 1060, 1089
Bsu36I CCTNAGG 1 cut(s) 555
BsuRI GGCC 1 cut(s) 114
BtgI CCRYGG 1 cut(s) 417
BtgZI GCGATG 1 cut(s) 444
BtrI CACGTC 1 cut(s) 276
BtsCI GGATG 5 cut(s) 178, 340, 342, 543, 661
Cac8I GCNNGC 3 cut(s) 86, 151, 299
CfoI GCGC 3 cut(s) 55, 247, 804
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 113, 916
Cfr9I CCCGGG 1 cut(s) 1199
Csp6I GTAC 1 cut(s) 1143
CviAII CATG 4 cut(s) 61, 577, 595, 1067
CviQI GTAC 1 cut(s) 1143
DdeI CTNAG 4 cut(s) 168, 555, 745, 947
DinI GGCGCC 1 cut(s) 803
DpnI GATC 5 cut(s) 371, 507, 570, 648, 903
DpnII GATC 5 cut(s) 369, 505, 568, 646, 901
Eam1104I CTCTTC 2 cut(s) 946, 1074
EarI CTCTTC 2 cut(s) 946, 1074
EciI GGCGGA 1 cut(s) 159
Eco130I CCWWGG 5 cut(s) 78, 109, 117, 1039, 1072
Eco24I GRGCYC 1 cut(s) 564
Eco47I GGWCC 1 cut(s) 916
Eco81I CCTNAGG 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 1199
EcoRII CCWGG 2 cut(s) 351, 804
EcoT14I CCWWGG 5 cut(s) 78, 109, 117, 1039, 1072
EcoT22I ATGCAT 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 564
EgeI GGCGCC 1 cut(s) 803
EheI GGCGCC 1 cut(s) 803
ErhI CCWWGG 5 cut(s) 78, 109, 117, 1039, 1072
FaeI CATG 4 cut(s) 64, 580, 598, 1070
FatI CATG 4 cut(s) 60, 576, 594, 1066
FauI CCCGC 1 cut(s) 989
FblI GTMKAC 4 cut(s) 278, 615, 1059, 1088
Fnu4HI GCNGC 4 cut(s) 51, 56, 75, 389
FokI GGATG 5 cut(s) 185, 327, 349, 530, 648
FriOI GRGCYC 1 cut(s) 564
Fsp4HI GCNGC 4 cut(s) 51, 56, 75, 389
FspBI CTAG 2 cut(s) 465, 657
FspI TGCGCA 1 cut(s) 246
GlaI GCGC 3 cut(s) 54, 246, 803
GluI GCNGC 4 cut(s) 51, 56, 75, 389
HaeII RGCGCY 2 cut(s) 56, 805
HaeIII GGCC 1 cut(s) 114
HapII CCGG 2 cut(s) 150, 1200
HhaI GCGC 3 cut(s) 55, 247, 804
Hin1I GRCGYC 2 cut(s) 93, 802
Hin1II CATG 4 cut(s) 64, 580, 598, 1070
Hin6I GCGC 3 cut(s) 53, 245, 802
HinP1I GCGC 3 cut(s) 53, 245, 802
HinfI GANTC 3 cut(s) 7, 19, 1111
HpaII CCGG 2 cut(s) 150, 1200
HphI GGTGA 2 cut(s) 433, 1088
Hpy166II GTNNAC 6 cut(s) 279, 616, 742, 1009, 1060, 1089
Hpy188I TCNGA 3 cut(s) 24, 316, 891
Hpy188III TCNNGA 7 cut(s) 100, 398, 644, 665, 700, 914, 1031
Hpy8I GTNNAC 6 cut(s) 279, 616, 742, 1009, 1060, 1089
HpyAV CCTTC 3 cut(s) 326, 886, 929
HpyCH4III ACNGT 3 cut(s) 283, 653, 978
HpyCH4IV ACGT 2 cut(s) 93, 275
HpyF10VI GCNNNNNNNGC 5 cut(s) 120, 150, 236, 267, 380
HpyF3I CTNAG 4 cut(s) 168, 555, 745, 947
HpySE526I ACGT 2 cut(s) 93, 275
Hsp92I GRCGYC 2 cut(s) 93, 802
Hsp92II CATG 4 cut(s) 64, 580, 598, 1070
HspAI GCGC 3 cut(s) 53, 245, 802
KasI GGCGCC 1 cut(s) 801
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Kzo9I GATC 5 cut(s) 369, 505, 568, 646, 901
LguI GCTCTTC 1 cut(s) 946
LmnI GCTCC 3 cut(s) 146, 559, 769
Lsp1109I GCAGC 2 cut(s) 42, 375
LweI GCATC 4 cut(s) 327, 349, 552, 670
MaeI CTAG 2 cut(s) 465, 657
MaeII ACGT 2 cut(s) 93, 275
MalI GATC 5 cut(s) 371, 507, 570, 648, 903
MboI GATC 5 cut(s) 369, 505, 568, 646, 901
MboII GAAGA 3 cut(s) 963, 1091, 1120
MfeI CAATTG 1 cut(s) 509
MhlI GDGCHC 2 cut(s) 125, 564
MluCI AATT 8 cut(s) 201, 219, 404, 509, 529, 605, 688, 897
Mly113I GGCGCC 1 cut(s) 802
MnlI CCTC 6 cut(s) 25, 107, 550, 625, 954, 994
Mph1103I ATGCAT 1 cut(s) 342
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 692
MseI TTAA 5 cut(s) 159, 528, 822, 1025, 1133
MslI CAYNNNNRTG 2 cut(s) 131, 345
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 2 cut(s) 150, 1200
MspR9I CCNGG 4 cut(s) 353, 806, 1200, 1201
MunI CAATTG 1 cut(s) 509
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 2 cut(s) 353, 806
MwoI GCNNNNNNNGC 5 cut(s) 120, 150, 236, 267, 380
NaeI GCCGGC 1 cut(s) 151
NarI GGCGCC 1 cut(s) 802
NciI CCSGG 2 cut(s) 1200, 1201
NdeII GATC 5 cut(s) 369, 505, 568, 646, 901
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 4 cut(s) 64, 580, 598, 1070
NlaIV GGNNCC 6 cut(s) 115, 124, 148, 271, 561, 803
NsbI TGCGCA 1 cut(s) 246
NsiI ATGCAT 1 cut(s) 342
NspI RCATGY 2 cut(s) 598, 1070
PciSI GCTCTTC 1 cut(s) 946
PctI GAATGC 1 cut(s) 31
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 692
PfeI GAWTC 3 cut(s) 7, 19, 1111
PkrI GCNGC 4 cut(s) 52, 57, 76, 390
PluTI GGCGCC 1 cut(s) 805
Psp6I CCWGG 2 cut(s) 351, 804
PspGI CCWGG 2 cut(s) 351, 804
PspN4I GGNNCC 6 cut(s) 115, 124, 148, 271, 561, 803
PspPI GGNCC 2 cut(s) 113, 916
PstI CTGCAG 1 cut(s) 393
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 1 cut(s) 1144
RsaNI GTAC 1 cut(s) 1143
RseI CAYNNNNRTG 2 cut(s) 131, 345
SapI GCTCTTC 1 cut(s) 946
SaqAI TTAA 5 cut(s) 159, 528, 822, 1025, 1133
SatI GCNGC 4 cut(s) 51, 56, 75, 389
Sau3AI GATC 5 cut(s) 369, 505, 568, 646, 901
Sau96I GGNCC 2 cut(s) 113, 916
ScrFI CCNGG 4 cut(s) 353, 806, 1200, 1201
SduI GDGCHC 2 cut(s) 125, 564
SfaNI GCATC 4 cut(s) 327, 349, 552, 670
SfcI CTRYAG 1 cut(s) 389
SfoI GGCGCC 1 cut(s) 803
SinI GGWCC 1 cut(s) 916
SmaI CCCGGG 1 cut(s) 1201
SmiMI CAYNNNNRTG 2 cut(s) 131, 345
SmlI CTYRAG 2 cut(s) 98, 398
SmoI CTYRAG 2 cut(s) 98, 398
Sse9I AATT 8 cut(s) 201, 219, 404, 509, 529, 605, 688, 897
SsiI CCGC 9 cut(s) 29, 50, 75, 144, 261, 547, 676, 767, 996
SspDI GGCGCC 1 cut(s) 801
SspI AATATT 1 cut(s) 1181
SspMI CTAG 2 cut(s) 465, 657
StyD4I CCNGG 4 cut(s) 351, 804, 1198, 1199
StyI CCWWGG 5 cut(s) 78, 109, 117, 1039, 1072
TaaI ACNGT 3 cut(s) 283, 653, 978
TaiI ACGT 2 cut(s) 96, 278
TaqI TCGA 3 cut(s) 5, 10, 645
TasI AATT 8 cut(s) 201, 219, 404, 509, 529, 605, 688, 897
TauI GCSGC 2 cut(s) 53, 77
TfiI GAWTC 3 cut(s) 7, 19, 1111
Tru1I TTAA 5 cut(s) 159, 528, 822, 1025, 1133
Tru9I TTAA 5 cut(s) 159, 528, 822, 1025, 1133
TseI GCWGC 2 cut(s) 55, 388
TspDTI ATGAA 9 cut(s) 77, 280, 320, 507, 565, 685, 686, 828, 1149
TspGWI ACGGA 2 cut(s) 247, 1003
TspMI CCCGGG 1 cut(s) 1199
VpaK11BI GGWCC 1 cut(s) 916
XapI RAATTY 3 cut(s) 201, 404, 605
XceI RCATGY 2 cut(s) 598, 1070
XmaI CCCGGG 1 cut(s) 1199
XmiI GTMKAC 4 cut(s) 278, 615, 1059, 1088
XmnI GAANNNNTTC 1 cut(s) 692
XspI CTAG 2 cut(s) 465, 657
ZraI GACGTC 1 cut(s) 94
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.