FvH4_1g11210
TCP Family

assists the folding of proteins upon ATP hydrolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003345
Physical Location & Seq
Reverse (-)
6120021 .. 6125324
5304 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g11210.t1

Sequence Viewer

Length: 1608 bp
ATGTCGCTGCGAGTCCTGAACCCTAACGCGGAGGTGCTGAACAAATCGGCGGCGCTGCACATGAACATCAACGCCGCCAAGGGCTTACAAGATGTGCTCAAAACCAACCTCGGCCCCAAAGGCACCATCAAGATGCTCGTCGGCGGTGCCGGAGACATCAAGCTCACCAAAGACGGCAACACTCTCCTCAAAGAAATGCAAATCCAGAACCCCACCGCCATCATGATTGCCAGGACTGCTGTCGCGCAAGATGACATCAGCGGCGACGGGACTACCTCTACTGTGCTGTTCATCGGCGAGCTGATGAAGCAGTCCGAGCGATATATTGATGAAGGGATGCATCCGCGTATGCTGGTGGATGGATTTGAGATTGCGAAGAGAGCAACGCTTCAGTTTATCGAGAAGTTTAAGACTCCGGTGGTGATGGGCAGTGAGCCTGACAAGGAGATCCTGAAAATGGTTGCAAGGACTACTGTGCGAACAAAGTTACATGAAGCATTGGCTGATCAATTGACCGACATTGTTGTAAATGCGGTTCTTTGTGTCCGCAAACCTGAGGAGGCAATTGATCTTTTTATGGTGGAGATTATGCACATGCGCCATAAATTCGATGCAGACACATGTTTGGTTGAGGGTCTTGTCCTTGATCATGGTTCTAGGCATCCTGATATGAAGCGACGAGCAGAGAATTGTTACATCTTGACTGCCAATGTATCTCTGGAGTATGAGAAAAGTGAAGTGAATTCTGGATTTTTCTATTCAAATGCGGAGCAGAGAGAAGCTATGGTTTTAGCTGAAAGACGTCAGGTTGACGAGAGAGTTAGAAAGATTATTGAGCTGAAAAATAAGGTTTGTTCTGGTAACGACAATAACTTTGTCGTGATCAATCAGAAGGGAATTGATCCCCCATCACTGGACCTTCTGGCAAGGGCAGGGATTATTGCTCTGAGAAGAGCAAAGAGGAGAAATATGGAACGGTTGGTTTTGGCTTGTGGAGGGGAGGCCGTGAACTCTGTAGATGATTTAACTCCTGATTGCCTTGGCTGGGCTGGACTTGTATACGAGCACGTCCTTGGTGAAGAGAAGTATACATTTGTCGAAAATGTGAAGAACCCTCATTCTTGCACAATTTTAATCAAAGGGCCTAATGACCATACAATTGCCCAGATTAAGGATGCTGTTCGCGACGGTCTAAGGGCAGTAAAAAATACAATAGAAGATGAAGCTGTAATCTTGGGTGCTGGAGCTTTTGAAGTGGCAGCCAGACAATATCTGATAAATGAAGTGAAGAAAACTGTTCAAGGGCGAGCTCAACTCGGTGTTGAAGCTTTTGCTGATGCGCTCCTTGTGGTGCCCAAGACACTCGCTGAGAATGCTGGCCTCGACACACAGGATGTGATAATTTCTCTCAAGGGAGAGCATGAGCGGGGAAATATTGTCGGTCTAAATCATAACACTGGGGAACCCATTGACCCACAAATGGAGGGGATCTTCGACAACTACTCTGTTAAGCGGCAGATTATAAACTCAGGGCCGGTAATTACATCTCAATTGTTGCTGGTGGATGAAGTGATCCGTGCTGGGCGCAACATGAGGAAGCCAACCTAA

Protein Analysis

536

Amino Acids

59.1

Weight (kDa)

6.12

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 29 - 528 1.8e-167 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1523
AatII GACGTC 1 cut(s) 805
AccB1I GGYRCC 3 cut(s) 122, 146, 1351
AccBSI CCGCTC 1 cut(s) 1426
AccI GTMKAC 2 cut(s) 1059, 1088
AccII CGCG 4 cut(s) 29, 245, 346, 1185
AclWI GGATC 4 cut(s) 442, 896, 1496, 1567
AcsI RAATTY 2 cut(s) 605, 742
AcuI CTGAAG 1 cut(s) 374
AcyI GRCGYC 1 cut(s) 802
AfiI CCNNNNNNNGG 6 cut(s) 28, 457, 913, 1045, 1171, 1480
AflIII ACRYGT 1 cut(s) 620
AgsI TTSAA 4 cut(s) 762, 1253, 1301, 1325
AjiI CACGTC 1 cut(s) 1069
AjnI CCWGG 1 cut(s) 230
AluBI AGCT 9 cut(s) 163, 301, 782, 794, 838, 1226, 1247, 1310, 1328
AluI AGCT 9 cut(s) 163, 301, 782, 794, 838, 1226, 1247, 1310, 1328
Alw21I GWGCWC 3 cut(s) 99, 1068, 1312
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 4 cut(s) 442, 896, 1496, 1567
AoxI GGCC 5 cut(s) 112, 1002, 1142, 1378, 1532
ApeKI GCWGC 3 cut(s) 7, 55, 1259
ApoI RAATTY 2 cut(s) 605, 742
ArsI GACNNNNNNTTYG 2 cut(s) 857, 889
AspLEI GCGC 5 cut(s) 55, 247, 600, 1342, 1587
AspS9I GGNCC 4 cut(s) 113, 916, 1142, 1532
AsuHPI GGTGA 3 cut(s) 157, 433, 1088
AvaII GGWCC 1 cut(s) 916
AxyI CCTNAGG 1 cut(s) 555
BaeGI GKGCMC 1 cut(s) 1356
BanI GGYRCC 3 cut(s) 122, 146, 1351
BanII GRGCYC 1 cut(s) 1312
Bbv12I GWGCWC 3 cut(s) 99, 1068, 1312
BbvI GCAGC 2 cut(s) 42, 1271
BccI CCATC 5 cut(s) 134, 227, 353, 418, 916
BceAI ACGGC 2 cut(s) 190, 989
BciT130I CCWGG 1 cut(s) 232
BclI TGATCA 3 cut(s) 505, 646, 882
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 1 cut(s) 657
BfmI CTRYAG 1 cut(s) 1014
BfoI RGCGCY 1 cut(s) 56
BglI GCCNNNNNGGC 1 cut(s) 120
BisI GCNGC 7 cut(s) 8, 51, 56, 75, 262, 1260, 1514
BlsI GCNGC 7 cut(s) 9, 52, 57, 76, 263, 1261, 1515
Bme1390I CCNGG 1 cut(s) 232
Bme18I GGWCC 1 cut(s) 916
BmgBI CACGTC 1 cut(s) 1069
BmgT120I GGNCC 4 cut(s) 113, 916, 1142, 1532
BmiI GGNNCC 5 cut(s) 115, 124, 148, 1353, 1464
BmrFI CCNGG 1 cut(s) 232
BmrI ACTGGG 1 cut(s) 1467
BmsI GCATC 7 cut(s) 123, 327, 349, 601, 670, 1165, 1327
BmuI ACTGGG 1 cut(s) 1467
BoxI GACNNNNGTC 1 cut(s) 239
BplI GAGNNNNNCTC 2 cut(s) 1299, 1331
BpmI CTGGAG 2 cut(s) 740, 1263
BpuEI CTTGAG 1 cut(s) 1394
BsaHI GRCGYC 1 cut(s) 802
BsaJI CCNNGG 4 cut(s) 78, 109, 1039, 1072
BsaWI WCCGGW 1 cut(s) 415
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 457, 913, 1045, 1171, 1480
Bse118I RCCGGY 1 cut(s) 1534
Bse1I ACTGG 2 cut(s) 918, 1462
Bse21I CCTNAGG 1 cut(s) 555
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 4 cut(s) 78, 109, 1039, 1072
BseGI GGATG 7 cut(s) 340, 342, 364, 661, 1180, 1399, 1570
BseLI CCNNNNNNNGG 6 cut(s) 28, 457, 913, 1045, 1171, 1480
BseMII CTCAG 4 cut(s) 546, 938, 1359, 1542
BseNI ACTGG 2 cut(s) 918, 1462
BseRI GAGGAG 3 cut(s) 176, 572, 976
BseSI GKGCMC 1 cut(s) 1356
BseXI GCAGC 2 cut(s) 42, 1271
BseYI CCCAGC 2 cut(s) 1044, 1580
BsgI GTGCAG 1 cut(s) 41
Bsh1236I CGCG 4 cut(s) 29, 245, 346, 1185
BshFI GGCC 5 cut(s) 114, 1004, 1144, 1380, 1534
BshNI GGYRCC 3 cut(s) 122, 146, 1351
BsiHKAI GWGCWC 3 cut(s) 99, 1068, 1312
BsiSI CCGG 3 cut(s) 150, 416, 1535
BslFI GGGAC 1 cut(s) 283
BslI CCNNNNNNNGG 6 cut(s) 28, 457, 913, 1045, 1171, 1480
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 283
BsmI GAATGC 1 cut(s) 1378
BsnI GGCC 5 cut(s) 114, 1004, 1144, 1380, 1534
Bsp1286I GDGCHC 4 cut(s) 99, 1068, 1312, 1356
Bsp143I GATC 8 cut(s) 447, 505, 568, 646, 882, 901, 1488, 1572
Bsp68I TCGCGA 1 cut(s) 1185
BspANI GGCC 5 cut(s) 114, 1004, 1144, 1380, 1534
BspCNI CTCAG 4 cut(s) 547, 939, 1360, 1541
BspFNI CGCG 4 cut(s) 29, 245, 346, 1185
BspHI TCATGA 1 cut(s) 222
BspLI GGNNCC 5 cut(s) 115, 124, 148, 1353, 1464
BspPI GGATC 4 cut(s) 442, 896, 1496, 1567
BspQI GCTCTTC 1 cut(s) 946
BspT107I GGYRCC 3 cut(s) 122, 146, 1351
BsrBI CCGCTC 1 cut(s) 1426
BsrFI RCCGGY 1 cut(s) 1534
BsrI ACTGG 2 cut(s) 918, 1462
BssAI RCCGGY 1 cut(s) 1534
BssECI CCNNGG 4 cut(s) 78, 109, 1039, 1072
BssMI GATC 8 cut(s) 447, 505, 568, 646, 882, 901, 1488, 1572
BssNAI GTATAC 2 cut(s) 1060, 1089
BssNI GRCGYC 1 cut(s) 802
BssT1I CCWWGG 3 cut(s) 78, 1039, 1072
Bst1107I GTATAC 2 cut(s) 1060, 1089
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 5 cut(s) 283, 475, 978, 1190, 1297
Bst6I CTCTTC 3 cut(s) 371, 946, 1074
BstACI GRCGYC 1 cut(s) 802
BstC8I GCNNGC 3 cut(s) 299, 1308, 1378
BstDEI CTNAG 5 cut(s) 555, 947, 1193, 1368, 1528
BstF5I GGATG 7 cut(s) 340, 342, 364, 661, 1180, 1399, 1570
BstFNI CGCG 4 cut(s) 29, 245, 346, 1185
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 5 cut(s) 55, 247, 600, 1342, 1587
BstKTI GATC 8 cut(s) 450, 508, 571, 649, 885, 904, 1491, 1575
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 8 cut(s) 447, 505, 568, 646, 882, 901, 1488, 1572
BstMWI GCNNNNNNNGC 6 cut(s) 120, 236, 307, 316, 380, 1373
BstNI CCWGG 1 cut(s) 232
BstNSI RCATGY 2 cut(s) 598, 624
BstPAI GACNNNNGTC 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 230
BstSFI CTRYAG 1 cut(s) 1014
BstSLI GKGCMC 1 cut(s) 1356
BstUI CGCG 4 cut(s) 29, 245, 346, 1185
BstV1I GCAGC 2 cut(s) 42, 1271
BstX2I RGATCY 2 cut(s) 447, 1488
BstYI RGATCY 2 cut(s) 447, 1488
BstZ17I GTATAC 2 cut(s) 1060, 1089
Bsu36I CCTNAGG 1 cut(s) 555
BsuRI GGCC 5 cut(s) 114, 1004, 1144, 1380, 1534
BtrI CACGTC 1 cut(s) 1069
BtsCI GGATG 7 cut(s) 340, 342, 364, 661, 1180, 1399, 1570
BtsI GCAGTG 1 cut(s) 436
BtsIMutI CAGTG 3 cut(s) 436, 911, 1455
BtuMI TCGCGA 1 cut(s) 1185
Cac8I GCNNGC 3 cut(s) 299, 1308, 1378
CciI TCATGA 1 cut(s) 222
CfoI GCGC 5 cut(s) 55, 247, 600, 1342, 1587
Cfr10I RCCGGY 1 cut(s) 1534
Cfr13I GGNCC 4 cut(s) 113, 916, 1142, 1532
CviAII CATG 8 cut(s) 61, 223, 491, 595, 621, 650, 1421, 1591
DdeI CTNAG 5 cut(s) 555, 947, 1193, 1368, 1528
DpnI GATC 8 cut(s) 449, 507, 570, 648, 884, 903, 1490, 1574
DpnII GATC 8 cut(s) 447, 505, 568, 646, 882, 901, 1488, 1572
Eam1104I CTCTTC 3 cut(s) 371, 946, 1074
EarI CTCTTC 3 cut(s) 371, 946, 1074
Ecl136II GAGCTC 1 cut(s) 1310
Eco130I CCWWGG 3 cut(s) 78, 1039, 1072
Eco24I GRGCYC 1 cut(s) 1312
Eco47I GGWCC 1 cut(s) 916
Eco53kI GAGCTC 1 cut(s) 1310
Eco57I CTGAAG 1 cut(s) 374
Eco81I CCTNAGG 1 cut(s) 555
EcoICRI GAGCTC 1 cut(s) 1310
EcoO109I RGGNCCY 1 cut(s) 1142
EcoRI GAATTC 1 cut(s) 742
EcoRII CCWGG 1 cut(s) 230
EcoT14I CCWWGG 3 cut(s) 78, 1039, 1072
EcoT22I ATGCAT 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 1312
ErhI CCWWGG 3 cut(s) 78, 1039, 1072
FaeI CATG 8 cut(s) 64, 226, 494, 598, 624, 653, 1424, 1594
FaqI GGGAC 1 cut(s) 283
FatI CATG 8 cut(s) 60, 222, 490, 594, 620, 649, 1420, 1590
FauI CCCGC 1 cut(s) 1419
FbaI TGATCA 3 cut(s) 505, 646, 882
FblI GTMKAC 2 cut(s) 1059, 1088
Fnu4HI GCNGC 7 cut(s) 8, 51, 56, 75, 262, 1260, 1514
FokI GGATG 7 cut(s) 327, 349, 371, 648, 1187, 1406, 1577
FriOI GRGCYC 1 cut(s) 1312
Fsp4HI GCNGC 7 cut(s) 8, 51, 56, 75, 262, 1260, 1514
FspBI CTAG 1 cut(s) 657
GlaI GCGC 5 cut(s) 54, 246, 599, 1341, 1586
GluI GCNGC 7 cut(s) 8, 51, 56, 75, 262, 1260, 1514
GsaI CCCAGC 2 cut(s) 1048, 1584
GsuI CTGGAG 2 cut(s) 740, 1263
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 5 cut(s) 114, 1004, 1144, 1380, 1534
HapII CCGG 3 cut(s) 150, 416, 1535
HhaI GCGC 5 cut(s) 55, 247, 600, 1342, 1587
Hin1I GRCGYC 1 cut(s) 802
Hin1II CATG 8 cut(s) 64, 226, 494, 598, 624, 653, 1424, 1594
Hin6I GCGC 5 cut(s) 53, 245, 598, 1340, 1585
HinP1I GCGC 5 cut(s) 53, 245, 598, 1340, 1585
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 1326
HinfI GANTC 2 cut(s) 12, 412
HpaII CCGG 3 cut(s) 150, 416, 1535
HphI GGTGA 3 cut(s) 157, 433, 1088
Hpy166II GTNNAC 4 cut(s) 811, 1009, 1060, 1089
Hpy188I TCNGA 4 cut(s) 316, 891, 948, 1275
Hpy8I GTNNAC 4 cut(s) 811, 1009, 1060, 1089
Hpy99I CGWCG 4 cut(s) 143, 269, 681, 1190
HpyAV CCTTC 3 cut(s) 326, 886, 929
HpyCH4III ACNGT 5 cut(s) 283, 475, 978, 1190, 1297
HpyCH4IV ACGT 2 cut(s) 802, 1068
HpyCH4V TGCA 7 cut(s) 58, 199, 340, 464, 592, 614, 1125
HpyF10VI GCNNNNNNNGC 6 cut(s) 120, 236, 307, 316, 380, 1373
HpyF3I CTNAG 5 cut(s) 555, 947, 1193, 1368, 1528
HpySE526I ACGT 2 cut(s) 802, 1068
Hsp92I GRCGYC 1 cut(s) 802
Hsp92II CATG 8 cut(s) 64, 226, 494, 598, 624, 653, 1424, 1594
HspAI GCGC 5 cut(s) 53, 245, 598, 1340, 1585
Ksp22I TGATCA 3 cut(s) 505, 646, 882
Kzo9I GATC 8 cut(s) 447, 505, 568, 646, 882, 901, 1488, 1572
LguI GCTCTTC 1 cut(s) 946
LmnI GCTCC 3 cut(s) 769, 1244, 1347
Lsp1109I GCAGC 2 cut(s) 42, 1271
LweI GCATC 7 cut(s) 123, 327, 349, 601, 670, 1165, 1327
MaeI CTAG 1 cut(s) 657
MaeII ACGT 2 cut(s) 802, 1068
MaeIII GTNAC 3 cut(s) 486, 692, 860
MalI GATC 8 cut(s) 449, 507, 570, 648, 884, 903, 1490, 1574
MbiI CCGCTC 1 cut(s) 1426
MboI GATC 8 cut(s) 447, 505, 568, 646, 882, 901, 1488, 1572
MboII GAAGA 7 cut(s) 388, 963, 1091, 1120, 1229, 1300, 1483
MfeI CAATTG 4 cut(s) 509, 564, 1158, 1550
MflI RGATCY 2 cut(s) 447, 1488
MhlI GDGCHC 4 cut(s) 99, 1068, 1312, 1356
MlyI GAGTC 2 cut(s) 21, 406
Mph1103I ATGCAT 1 cut(s) 342
MseI TTAA 5 cut(s) 408, 1025, 1133, 1170, 1509
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 261
MspI CCGG 3 cut(s) 150, 416, 1535
MspR9I CCNGG 1 cut(s) 232
MunI CAATTG 4 cut(s) 509, 564, 1158, 1550
Mva1269I GAATGC 1 cut(s) 1378
MvaI CCWGG 1 cut(s) 232
MvnI CGCG 4 cut(s) 29, 245, 346, 1185
MwoI GCNNNNNNNGC 6 cut(s) 120, 236, 307, 316, 380, 1373
NdeII GATC 8 cut(s) 447, 505, 568, 646, 882, 901, 1488, 1572
NlaIII CATG 8 cut(s) 64, 226, 494, 598, 624, 653, 1424, 1594
NlaIV GGNNCC 5 cut(s) 115, 124, 148, 1353, 1464
NmeAIII GCCGAG 1 cut(s) 90
NruI TCGCGA 1 cut(s) 1185
NsiI ATGCAT 1 cut(s) 342
NspI RCATGY 2 cut(s) 598, 624
PagI TCATGA 1 cut(s) 222
PciI ACATGT 1 cut(s) 620
PciSI GCTCTTC 1 cut(s) 946
PctI GAATGC 1 cut(s) 1378
PkrI GCNGC 7 cut(s) 9, 52, 57, 76, 263, 1261, 1515
PleI GAGTC 2 cut(s) 20, 406
PpsI GAGTC 2 cut(s) 20, 406
PscI ACATGT 1 cut(s) 620
PshAI GACNNNNGTC 1 cut(s) 239
PsiI TTATAA 1 cut(s) 1523
Psp124BI GAGCTC 1 cut(s) 1312
Psp6I CCWGG 1 cut(s) 230
PspFI CCCAGC 2 cut(s) 1044, 1580
PspGI CCWGG 1 cut(s) 230
PspN4I GGNNCC 5 cut(s) 115, 124, 148, 1353, 1464
PspPI GGNCC 4 cut(s) 113, 916, 1142, 1532
PsrI GAACNNNNNNTAC 2 cut(s) 519, 551
PsuI RGATCY 2 cut(s) 447, 1488
RruI TCGCGA 1 cut(s) 1185
RseI CAYNNNNRTG 1 cut(s) 131
SacI GAGCTC 1 cut(s) 1312
SapI GCTCTTC 1 cut(s) 946
SaqAI TTAA 5 cut(s) 408, 1025, 1133, 1170, 1509
SatI GCNGC 7 cut(s) 8, 51, 56, 75, 262, 1260, 1514
Sau3AI GATC 8 cut(s) 447, 505, 568, 646, 882, 901, 1488, 1572
Sau96I GGNCC 4 cut(s) 113, 916, 1142, 1532
SchI GAGTC 2 cut(s) 21, 406
ScrFI CCNGG 1 cut(s) 232
SduI GDGCHC 4 cut(s) 99, 1068, 1312, 1356
SfaNI GCATC 7 cut(s) 123, 327, 349, 601, 670, 1165, 1327
SfcI CTRYAG 1 cut(s) 1014
SinI GGWCC 1 cut(s) 916
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 1409
SmoI CTYRAG 1 cut(s) 1409
SspI AATATT 1 cut(s) 1435
SspMI CTAG 1 cut(s) 657
SstI GAGCTC 1 cut(s) 1312
StyD4I CCNGG 1 cut(s) 230
StyI CCWWGG 3 cut(s) 78, 1039, 1072
TaaI ACNGT 5 cut(s) 283, 475, 978, 1190, 1297
TaiI ACGT 2 cut(s) 805, 1071
TaqI TCGA 5 cut(s) 399, 609, 1098, 1383, 1494
TaqII GACCGA 2 cut(s) 530, 1430
TauI GCSGC 4 cut(s) 53, 77, 264, 1516
Tru1I TTAA 5 cut(s) 408, 1025, 1133, 1170, 1509
Tru9I TTAA 5 cut(s) 408, 1025, 1133, 1170, 1509
TscAI CASTG 3 cut(s) 436, 918, 1462
TseI GCWGC 3 cut(s) 7, 55, 1259
TspDTI ATGAA 9 cut(s) 77, 280, 320, 345, 507, 686, 1236, 1296, 1581
TspGWI ACGGA 1 cut(s) 1565
TspRI CASTG 3 cut(s) 436, 918, 1462
VpaK11BI GGWCC 1 cut(s) 916
XapI RAATTY 2 cut(s) 605, 742
XceI RCATGY 2 cut(s) 598, 624
XcmI CCANNNNNNNNNTGG 1 cut(s) 715
XmiI GTMKAC 2 cut(s) 1059, 1088
XspI CTAG 1 cut(s) 657
ZraI GACGTC 1 cut(s) 803
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.