AT3G03030

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003780
Physical Location & Seq
Forward (+)
682054 .. 684083
2030 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03030.2

Sequence Viewer

Length: 1425 bp
ATGGATCTAGTGAGCAGTTTACCAGACGATGTTCGTTGTCATATACTGTCCTTCCTTACCACAAAGGAGTCTGCTTTGACATCTGTTCTGTCTAAGAAGTGGCGGAATCTGTTCACGCTTGTCCCTAATCTCGACTTGGATGACTCTGAGTATCTTCATCCGGAAGAGACTAAGTGGGAAAGAGAAGAAATTCTGGATAGCTTCTTGGATTTTGTGGAAAGAGTATTGTCTTTGCAGGGAAATGGTCCCATTCGTAGGTTCTCCCTCAGGTGTGAAAGCGGTGTTCCTCCAGCTCGTCTGAATCGTTGGTTATGTAAAGTGCTGCAGCGTGGTGTTGCGGACCTTGAACTTATCATCGACTTCGAAGATGGTTATCTTCTGCCTCGAGAGTTGTTCGTCAGTGAGACACTCGTCAATCTCAGGTTAAAATCTGAATTTGGTTGTATTCATTGGTGGCCTGGAGCTGAAGGCACTTTCTTACCAAGGCTTCAAAGTCTTGATATAAGCTCTGCGATGGTTTTTTGTGATGATAAGCTTCAGAAGCTTCTTCCTTGTTTCCCTGTGATTGAGGAATTACGGTTGGTAGGTATGGAGTGGATTGATTCTCATGTAACCGTGTCGTGTGCAACCCTCACTAATCAGCTACTTCTTAGCTCCACCGGTTCCAGAACCTCTAAAAATCCAAAGAGCGTTTCTTTTGATACTCCAAATCTGCTTTCCCTAGCCTACTCTGATTTAGTTGCTGAGGACTATCCGTTGGTTAATATGAAAAGTTTATTCAAGGCTCGAATCATCCTTGGAGTAGATGACGATCAAATCGCCCGAATGCGAGCCCCAACTAACTATTTGTCAGAGGATGATGTTGTTCGCCGATTTGGCAATGTGGTGAAGCTCATGAAGGGCATACAAAATGTTCAGCAACTTCACTTGCATTCTGATACACTCGAGGTGCTTTCAATGTGCTGTGAATCGATGCCGGTGTTCAACAACGTCAAAACGTTAGGCATTAAGAGTGACTACGACAGAGGATGGCAAGCAGTGCCTGCTCTTCTAAGGAATTGTCCACATCTAGAAACCCTGACCTTTGAGGGTCTCGTGCACCATGTGACAGATAAATGTGGAGATGCTTGTGACTGCATTTTCCGGAAGGATAAAGGCCGTTCGCTCAAATCTTGTCCAGTAAAAGTGGTAGAGATTAAAGAGTTTAGAGTAACAATGAAAGAGATGCACCTGATAGAGCATTTCTTGGACAACTTTCCATGTTTGAAGGAGATGAAGATCTATAGAGAAGAGTATGATCCTTCACCGTTTGGAAACGACCCTCAAGTGGATGATCTTATCTTGGACATGATAGAAGAGTACAAGGATATATATAGTTGCAATGTCCAACTCTTGGAGCCGTGTGACAAGTGGGATGAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

474

Amino Acids

54.39

Weight (kDa)

5.03

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 5 - 42 1.5e-07 F-box domain
LRR_At5g56370 PF24758 100 - 242 5.2e-09 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1393
AccIII TCCGGA 2 cut(s) 160, 1143
AciI CCGC 3 cut(s) 103, 279, 338
AclI AACGTT 1 cut(s) 998
AclWI GGATC 2 cut(s) 12, 1292
AcsI RAATTY 2 cut(s) 189, 434
AcuI CTGAAG 2 cut(s) 486, 521
AdeI CACNNNGTG 1 cut(s) 1105
AfaI GTAC 1 cut(s) 1361
AfiI CCNNNNNNNGG 3 cut(s) 255, 1327, 1393
AgeI ACCGGT 1 cut(s) 659
AgsI TTSAA 6 cut(s) 347, 491, 781, 957, 985, 1267
AjnI CCWGG 1 cut(s) 457
AjuI GAANNNNNNNTTGG 4 cut(s) 676, 700, 708, 732
AluBI AGCT 9 cut(s) 201, 293, 464, 507, 535, 544, 643, 654, 892
AluI AGCT 9 cut(s) 201, 293, 464, 507, 535, 544, 643, 654, 892
Alw21I GWGCWC 1 cut(s) 1101
Alw26I GTCTC 3 cut(s) 161, 398, 1097
Alw44I GTGCAC 1 cut(s) 1097
AlwI GGATC 2 cut(s) 12, 1292
AlwNI CAGNNNCTG 1 cut(s) 1043
Ama87I CYCGRG 2 cut(s) 384, 944
Aor13HI TCCGGA 2 cut(s) 160, 1143
AoxI GGCC 2 cut(s) 455, 1156
ApaLI GTGCAC 1 cut(s) 1097
ApeKI GCWGC 2 cut(s) 322, 325
ApoI RAATTY 2 cut(s) 189, 434
AsiGI ACCGGT 1 cut(s) 659
Asp700I GAANNNNTTC 2 cut(s) 110, 189
AspS9I GGNCC 2 cut(s) 245, 340
AsuHPI GGTGA 2 cut(s) 898, 1296
AsuII TTCGAA 1 cut(s) 363
AvaI CYCGRG 2 cut(s) 384, 944
AvaII GGWCC 2 cut(s) 245, 340
AxyI CCTNAGG 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 1101
BanII GRGCYC 1 cut(s) 835
BauI CACGAG 1 cut(s) 1094
Bbv12I GWGCWC 1 cut(s) 1101
BbvCI CCTCAGC 1 cut(s) 744
BbvI GCAGC 2 cut(s) 309, 337
BccI CCATC 3 cut(s) 362, 508, 1023
BceAI ACGGC 2 cut(s) 1143, 1384
BciT130I CCWGG 1 cut(s) 459
BcoDI GTCTC 3 cut(s) 161, 398, 1097
BfaI CTAG 3 cut(s) 8, 722, 1070
BfmI CTRYAG 2 cut(s) 323, 1282
BglI GCCNNNNNGGC 1 cut(s) 876
BglII AGATCT 1 cut(s) 1278
BisI GCNGC 2 cut(s) 323, 326
BlsI GCNGC 2 cut(s) 324, 327
Bme1390I CCNGG 1 cut(s) 459
Bme18I GGWCC 2 cut(s) 245, 340
BmeT110I CYCGRG 2 cut(s) 384, 944
BmgT120I GGNCC 2 cut(s) 245, 340
BmiI GGNNCC 3 cut(s) 247, 664, 1398
BmrFI CCNGG 1 cut(s) 459
BmsI GCATC 3 cut(s) 963, 1114, 1215
BoxI GACNNNNGTC 1 cut(s) 410
BpmI CTGGAG 2 cut(s) 273, 480
Bpu10I CCTNAGC 1 cut(s) 744
Bpu14I TTCGAA 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 1308
Bsa29I ATCGAT 1 cut(s) 971
BsaBI GATNNNNATC 3 cut(s) 372, 810, 1277
BsaI GGTCTC 1 cut(s) 1097
BsaJI CCNNGG 2 cut(s) 482, 796
BsaWI WCCGGW 3 cut(s) 160, 659, 1143
Bsc4I CCNNNNNNNGG 3 cut(s) 255, 1327, 1393
Bse118I RCCGGY 2 cut(s) 659, 976
Bse1I ACTGG 1 cut(s) 1178
Bse21I CCTNAGG 1 cut(s) 266
Bse3DI GCAATG 2 cut(s) 886, 1387
Bse8I GATNNNNATC 3 cut(s) 372, 810, 1277
BseAI TCCGGA 2 cut(s) 160, 1143
BseBI CCWGG 1 cut(s) 459
BseCI ATCGAT 1 cut(s) 971
BseDI CCNNGG 2 cut(s) 482, 796
BseGI GGATG 7 cut(s) 145, 157, 792, 862, 1034, 1336, 1420
BseJI GATNNNNATC 3 cut(s) 372, 810, 1277
BseLI CCNNNNNNNGG 3 cut(s) 255, 1327, 1393
BseMI GCAATG 2 cut(s) 886, 1387
BseMII CTCAG 4 cut(s) 138, 280, 433, 735
BseNI ACTGG 1 cut(s) 1178
BseSI GKGCMC 1 cut(s) 1101
BseXI GCAGC 2 cut(s) 309, 337
BshFI GGCC 2 cut(s) 457, 1158
BshTI ACCGGT 1 cut(s) 659
BshVI ATCGAT 1 cut(s) 971
BsiHKAI GWGCWC 1 cut(s) 1101
BsiHKCI CYCGRG 2 cut(s) 384, 944
BsiSI CCGG 4 cut(s) 161, 660, 977, 1144
BslFI GGGAC 2 cut(s) 107, 231
BslI CCNNNNNNNGG 3 cut(s) 255, 1327, 1393
BsmAI GTCTC 3 cut(s) 161, 398, 1097
BsmFI GGGAC 2 cut(s) 107, 231
BsmI GAATGC 2 cut(s) 831, 931
BsnI GGCC 2 cut(s) 457, 1158
Bso31I GGTCTC 1 cut(s) 1097
BsoBI CYCGRG 2 cut(s) 384, 944
Bsp119I TTCGAA 1 cut(s) 363
Bsp1286I GDGCHC 2 cut(s) 835, 1101
Bsp13I TCCGGA 2 cut(s) 160, 1143
Bsp143I GATC 5 cut(s) 4, 811, 1278, 1297, 1333
BspACI CCGC 3 cut(s) 103, 279, 338
BspANI GGCC 2 cut(s) 457, 1158
BspCNI CTCAG 4 cut(s) 139, 279, 432, 736
BspDI ATCGAT 1 cut(s) 971
BspEI TCCGGA 2 cut(s) 160, 1143
BspHI TCATGA 1 cut(s) 894
BspLI GGNNCC 3 cut(s) 247, 664, 1398
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 2 cut(s) 12, 1292
BspQI GCTCTTC 1 cut(s) 1053
BspT104I TTCGAA 1 cut(s) 363
BspTNI GGTCTC 1 cut(s) 1097
BsrDI GCAATG 2 cut(s) 886, 1387
BsrFI RCCGGY 2 cut(s) 659, 976
BsrI ACTGG 1 cut(s) 1178
BssAI RCCGGY 2 cut(s) 659, 976
BssECI CCNNGG 2 cut(s) 482, 796
BssMI GATC 5 cut(s) 4, 811, 1278, 1297, 1333
BssSI CACGAG 1 cut(s) 1094
BssT1I CCWWGG 2 cut(s) 482, 796
Bst2BI CACGAG 1 cut(s) 1094
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 4 cut(s) 48, 579, 616, 1308
Bst6I CTCTTC 4 cut(s) 159, 1053, 1284, 1350
BstAPI GCANNNNNTGC 2 cut(s) 1039, 1043
BstBI TTCGAA 1 cut(s) 363
BstC8I GCNNGC 3 cut(s) 831, 1035, 1044
BstDEI CTNAG 8 cut(s) 93, 147, 171, 266, 419, 650, 744, 1052
BstF5I GGATG 7 cut(s) 145, 157, 792, 862, 1034, 1336, 1420
BstKTI GATC 5 cut(s) 7, 814, 1281, 1300, 1336
BstMAI GTCTC 3 cut(s) 161, 398, 1097
BstMBI GATC 5 cut(s) 4, 811, 1278, 1297, 1333
BstMWI GCNNNNNNNGC 4 cut(s) 541, 876, 1039, 1043
BstNI CCWGG 1 cut(s) 459
BstPAI GACNNNNGTC 1 cut(s) 410
BstSCI CCNGG 1 cut(s) 457
BstSFI CTRYAG 2 cut(s) 323, 1282
BstSLI GKGCMC 1 cut(s) 1101
BstV1I GCAGC 2 cut(s) 309, 337
BstX2I RGATCY 2 cut(s) 4, 1278
BstYI RGATCY 2 cut(s) 4, 1278
Bsu15I ATCGAT 1 cut(s) 971
Bsu36I CCTNAGG 1 cut(s) 266
BsuRI GGCC 2 cut(s) 457, 1158
BsuTUI ATCGAT 1 cut(s) 971
BtgZI GCGATG 1 cut(s) 527
BtsCI GGATG 7 cut(s) 145, 157, 792, 862, 1034, 1336, 1420
BtsI GCAGTG 1 cut(s) 1044
BtsIMutI CAGTG 2 cut(s) 406, 1044
Cac8I GCNNGC 3 cut(s) 831, 1035, 1044
CaiI CAGNNNCTG 1 cut(s) 1043
CciI TCATGA 1 cut(s) 894
Cfr10I RCCGGY 2 cut(s) 659, 976
Cfr13I GGNCC 2 cut(s) 245, 340
ClaI ATCGAT 1 cut(s) 971
Csp6I GTAC 1 cut(s) 1360
CspAI ACCGGT 1 cut(s) 659
CviAII CATG 5 cut(s) 608, 895, 1103, 1260, 1348
CviQI GTAC 1 cut(s) 1360
DdeI CTNAG 8 cut(s) 93, 147, 171, 266, 419, 650, 744, 1052
DpnI GATC 5 cut(s) 6, 813, 1280, 1299, 1335
DpnII GATC 5 cut(s) 4, 811, 1278, 1297, 1333
DraIII CACNNNGTG 1 cut(s) 1105
Eam1104I CTCTTC 4 cut(s) 159, 1053, 1284, 1350
EarI CTCTTC 4 cut(s) 159, 1053, 1284, 1350
EciI GGCGGA 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 482, 796
Eco24I GRGCYC 1 cut(s) 835
Eco31I GGTCTC 1 cut(s) 1097
Eco47I GGWCC 2 cut(s) 245, 340
Eco57I CTGAAG 2 cut(s) 486, 521
Eco81I CCTNAGG 1 cut(s) 266
Eco88I CYCGRG 2 cut(s) 384, 944
EcoRII CCWGG 1 cut(s) 457
EcoT14I CCWWGG 2 cut(s) 482, 796
EcoT38I GRGCYC 1 cut(s) 835
ErhI CCWWGG 2 cut(s) 482, 796
FaeI CATG 5 cut(s) 611, 898, 1106, 1263, 1351
FaqI GGGAC 2 cut(s) 107, 231
FatI CATG 5 cut(s) 607, 894, 1102, 1259, 1347
Fnu4HI GCNGC 2 cut(s) 323, 326
FokI GGATG 6 cut(s) 144, 152, 779, 869, 1041, 1343
FriOI GRGCYC 1 cut(s) 835
Fsp4HI GCNGC 2 cut(s) 323, 326
FspBI CTAG 3 cut(s) 8, 722, 1070
GluI GCNGC 2 cut(s) 323, 326
GsuI CTGGAG 2 cut(s) 273, 480
HaeIII GGCC 2 cut(s) 457, 1158
HapII CCGG 4 cut(s) 161, 660, 977, 1144
Hin1II CATG 5 cut(s) 611, 898, 1106, 1263, 1351
HindIII AAGCTT 2 cut(s) 533, 542
HinfI GANTC 7 cut(s) 68, 106, 143, 301, 602, 789, 968
HpaII CCGG 4 cut(s) 161, 660, 977, 1144
HphI GGTGA 2 cut(s) 898, 1296
Hpy166II GTNNAC 4 cut(s) 20, 114, 1064, 1099
Hpy188I TCNGA 7 cut(s) 148, 300, 433, 540, 733, 853, 937
Hpy188III TCNNGA 9 cut(s) 131, 161, 194, 386, 497, 666, 895, 1070, 1144
Hpy8I GTNNAC 4 cut(s) 20, 114, 1064, 1099
HpyAV CCTTC 6 cut(s) 61, 461, 892, 1141, 1261, 1311
HpyCH4III ACNGT 4 cut(s) 48, 579, 616, 1308
HpyCH4IV ACGT 2 cut(s) 990, 998
HpyCH4V TGCA 8 cut(s) 235, 325, 626, 931, 1099, 1137, 1228, 1380
HpyF10VI GCNNNNNNNGC 4 cut(s) 541, 876, 1039, 1043
HpyF3I CTNAG 8 cut(s) 93, 147, 171, 266, 419, 650, 744, 1052
HpySE526I ACGT 2 cut(s) 990, 998
Hsp92II CATG 5 cut(s) 611, 898, 1106, 1263, 1351
Kpn2I TCCGGA 2 cut(s) 160, 1143
Kzo9I GATC 5 cut(s) 4, 811, 1278, 1297, 1333
LguI GCTCTTC 1 cut(s) 1053
LmnI GCTCC 3 cut(s) 461, 659, 1396
Lsp1109I GCAGC 2 cut(s) 309, 337
LweI GCATC 3 cut(s) 963, 1114, 1215
MaeI CTAG 3 cut(s) 8, 722, 1070
MaeII ACGT 2 cut(s) 990, 998
MaeIII GTNAC 6 cut(s) 610, 1013, 1105, 1130, 1210, 1403
MalI GATC 5 cut(s) 6, 813, 1280, 1299, 1335
MboI GATC 5 cut(s) 4, 811, 1278, 1297, 1333
MflI RGATCY 2 cut(s) 4, 1278
MhlI GDGCHC 2 cut(s) 835, 1101
MluCI AATT 4 cut(s) 189, 434, 572, 1057
MlyI GAGTC 2 cut(s) 77, 137
MmeI TCCRAC 1 cut(s) 1411
MroI TCCGGA 2 cut(s) 160, 1143
MroXI GAANNNNTTC 2 cut(s) 110, 189
MseI TTAA 4 cut(s) 425, 762, 1008, 1197
MspI CCGG 4 cut(s) 161, 660, 977, 1144
MspR9I CCNGG 1 cut(s) 459
Mva1269I GAATGC 2 cut(s) 831, 931
MvaI CCWGG 1 cut(s) 459
MwoI GCNNNNNNNGC 4 cut(s) 541, 876, 1039, 1043
NdeII GATC 5 cut(s) 4, 811, 1278, 1297, 1333
NlaIII CATG 5 cut(s) 611, 898, 1106, 1263, 1351
NlaIV GGNNCC 3 cut(s) 247, 664, 1398
NmuCI GTSAC 4 cut(s) 1013, 1105, 1130, 1403
NspV TTCGAA 1 cut(s) 363
PaeR7I CTCGAG 2 cut(s) 384, 944
PagI TCATGA 1 cut(s) 894
PciSI GCTCTTC 1 cut(s) 1053
PcsI WCGNNNNNNNCGW 1 cut(s) 301
PctI GAATGC 2 cut(s) 831, 931
PdmI GAANNNNTTC 2 cut(s) 110, 189
PfeI GAWTC 5 cut(s) 106, 301, 602, 789, 968
PflMI CCANNNNNTGG 1 cut(s) 1393
PinAI ACCGGT 1 cut(s) 659
PkrI GCNGC 2 cut(s) 324, 327
PleI GAGTC 2 cut(s) 76, 137
PpsI GAGTC 2 cut(s) 76, 137
PshAI GACNNNNGTC 1 cut(s) 410
Psp1406I AACGTT 1 cut(s) 998
Psp6I CCWGG 1 cut(s) 457
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 3 cut(s) 247, 664, 1398
PspPI GGNCC 2 cut(s) 245, 340
PspXI VCTCGAGB 1 cut(s) 944
PstI CTGCAG 1 cut(s) 327
PstNI CAGNNNCTG 1 cut(s) 1043
PsuI RGATCY 2 cut(s) 4, 1278
RsaI GTAC 1 cut(s) 1361
RsaNI GTAC 1 cut(s) 1360
SapI GCTCTTC 1 cut(s) 1053
SaqAI TTAA 4 cut(s) 425, 762, 1008, 1197
SatI GCNGC 2 cut(s) 323, 326
Sau3AI GATC 5 cut(s) 4, 811, 1278, 1297, 1333
Sau96I GGNCC 2 cut(s) 245, 340
SchI GAGTC 2 cut(s) 77, 137
ScrFI CCNGG 1 cut(s) 459
SduI GDGCHC 2 cut(s) 835, 1101
SfaNI GCATC 3 cut(s) 963, 1114, 1215
SfcI CTRYAG 2 cut(s) 323, 1282
Sfr274I CTCGAG 2 cut(s) 384, 944
SfuI TTCGAA 1 cut(s) 363
SinI GGWCC 2 cut(s) 245, 340
SlaI CTCGAG 2 cut(s) 384, 944
SmlI CTYRAG 3 cut(s) 384, 944, 1323
SmoI CTYRAG 3 cut(s) 384, 944, 1323
Sse9I AATT 4 cut(s) 189, 434, 572, 1057
SsiI CCGC 3 cut(s) 103, 279, 338
SspMI CTAG 3 cut(s) 8, 722, 1070
StyD4I CCNGG 1 cut(s) 457
StyI CCWWGG 2 cut(s) 482, 796
TaaI ACNGT 4 cut(s) 48, 579, 616, 1308
TaiI ACGT 2 cut(s) 993, 1001
TaqI TCGA 7 cut(s) 132, 357, 363, 385, 787, 945, 971
TasI AATT 4 cut(s) 189, 434, 572, 1057
TatI WGTACW 1 cut(s) 1359
TfiI GAWTC 5 cut(s) 106, 301, 602, 789, 968
Tru1I TTAA 4 cut(s) 425, 762, 1008, 1197
Tru9I TTAA 4 cut(s) 425, 762, 1008, 1197
TscAI CASTG 2 cut(s) 406, 1044
TseFI GTSAC 4 cut(s) 1013, 1105, 1130, 1403
TseI GCWGC 2 cut(s) 322, 325
Tsp45I GTSAC 4 cut(s) 1013, 1105, 1130, 1403
TspDTI ATGAA 6 cut(s) 146, 437, 782, 911, 1232, 1289
TspGWI ACGGA 1 cut(s) 744
TspRI CASTG 2 cut(s) 406, 1044
Van91I CCANNNNNTGG 1 cut(s) 1393
VneI GTGCAC 1 cut(s) 1097
VpaK11BI GGWCC 2 cut(s) 245, 340
XapI RAATTY 2 cut(s) 189, 434
XbaI TCTAGA 1 cut(s) 1069
XhoI CTCGAG 2 cut(s) 384, 944
XmnI GAANNNNTTC 2 cut(s) 110, 189
XspI CTAG 3 cut(s) 8, 722, 1070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.