AT3G58880

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003780
Physical Location & Seq
Reverse (-)
21768485 .. 21770167
1683 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G58880.1

Sequence Viewer

Length: 1365 bp
ATGGTGGATCTTGTTAGCAGTCTACCAGATGATCTCCTTGGTCATATATTGTCCTTACTTACCACAAAGGAGGCTGCTTTGACTTCTATTCTCTCCAAGAGGTGGCGCTATCTCATTGCTTTTGTCCCTTATCTCGAGTTTGATGACTCTGCCTTTCTGAACCCAGAAGAAGGTAAACAGACAAGGGAAGGAACTCGACAGAGCTTTATAGATTTTGTGGATAGAGTATTGGCTTTGCATGGAGATTCTCCCATCAGAAAATTCTCACTCAAGTGTAAAACCGGTGTTGATTTAGATCTTCTGAATCAGTGGATATGTAATGTGCTGCAGCGCGGTGTTTTGCTCATTGATCTGTCCATGGATTTGGGACATAGATGTATGTTCATCGAAATTTTCATGAGCAGAACACTAGTTGAACTGAAATTAGGAAGTGGATGTCGTATTGCTTTTGGGCCTGAACACATTTCGGCCTTACCTATGCTTAAGACTCTCACTCTTGACTCAGTTAGTTGGTCTGATAGTGGTCAGCTTGAGAGGCTACTTTCTGCTTGCCCTGCACTCGAGGCATTAAACTTGGCTAATGTGCATGGGTCATATCCGAATGCGACCGTGTCAATTGCAAGCCTCAAGACTCTAACAATTAAGTCTGTCAGTTTGTCAGGCCCTGCACACGTTTTCTCATTTGATACACCGAATCTTCTTTGCTTAAATTACACTGCATTGTTTGAGGACGACTATCCATTAGTTAATTTGGAATACTTAGTTGAGGCTCAAATCAAGTTTGTATTAACTGATCGTCTAATCAAGCTAGTAAGCGTCCGAAAGAATGGTCTTCTCATGCTTAGTGAAGTGCAGAAACTTATTCGTGGCATATCCAGTGTTCGGAAGCTTTACTTATCTCCCGGGACTCTCCAGGTGCTTGGCCAATGCTCTCAGGCAATGCCGGTGTTCAACAACCTCACATTCTTAGTCATTGAGAGTAGCATGGATATAAGATGGCAAGCAATGCCGGTTCTCTTGAAAAATTGTCCACGTCTAGAAACTCTAGTCATTAAGGGTGGTCTCGTACACTGTGTCGCAGCTGACTGCGGGGACGCTTGTACCTGCATTTCTCGGGAGGAGAAAGGCCGTTCGCTTGCATCTTGTCCAGTGAAGCGGTTAGAGATTCGTGAGTTTCAAGGAACGTTGAGAGAGATGGAAATGATCAAGCATTTCTATTACTGTTTCCTCTGTTTGAAGGAGATGGAGATCTATGTTAAAGATGGTCGTCCACAGTTCCTAGCCCCTTTAGCGTCGGATTCTAACCCATTCAAGCACTTATTGGGTCGCAATGTTATAATCAAGGTGCACGGTTCTTTGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

454

Amino Acids

50.76

Weight (kDa)

7.5

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 6 - 40 3.1e-07 F-box domain
LRR_At5g56370 PF24758 126 - 233 9.4e-11 FBD-associated F-box protein At5g56370, LRR repeats
LRR_2 PF07723 160 - 185 2e-07 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1337
Acc36I ACCTGC 1 cut(s) 1112
AccB7I CCANNNNNTGG 1 cut(s) 102
AccI GTMKAC 1 cut(s) 22
AccII CGCG 1 cut(s) 333
AciI CCGC 3 cut(s) 333, 1089, 1156
AclI AACGTT 1 cut(s) 1184
AclWI GGATC 1 cut(s) 15
AcoI YGGCCR 1 cut(s) 922
AcsI RAATTY 2 cut(s) 260, 390
AfaI GTAC 2 cut(s) 1068, 1102
AfiI CCNNNNNNNGG 3 cut(s) 102, 170, 882
AflII CTTAAG 1 cut(s) 482
AflIII ACRYGT 1 cut(s) 670
AgeI ACCGGT 1 cut(s) 281
AgsI TTSAA 6 cut(s) 416, 952, 1021, 1178, 1237, 1312
AhlI ACTAGT 1 cut(s) 409
AjiI CACGTC 1 cut(s) 1034
AjnI CCWGG 1 cut(s) 912
AleI CACNNNNGTG 1 cut(s) 271
AluBI AGCT 5 cut(s) 204, 529, 808, 889, 1082
AluI AGCT 5 cut(s) 204, 529, 808, 889, 1082
Alw21I GWGCWC 1 cut(s) 1350
Alw26I GTCTC 1 cut(s) 1067
Alw44I GTGCAC 1 cut(s) 1346
AlwI GGATC 1 cut(s) 15
AlwNI CAGNNNCTG 1 cut(s) 665
Ama87I CYCGRG 4 cut(s) 134, 560, 902, 1113
AoxI GGCC 5 cut(s) 452, 468, 661, 922, 1126
ApaLI GTGCAC 1 cut(s) 1346
ApeKI GCWGC 4 cut(s) 74, 325, 328, 1079
ApoI RAATTY 2 cut(s) 260, 390
ArsI GACNNNNNNTTYG 2 cut(s) 814, 846
AsiGI ACCGGT 1 cut(s) 281
AspLEI GCGC 2 cut(s) 108, 333
AspS9I GGNCC 2 cut(s) 452, 662
AsuC2I CCSGG 2 cut(s) 903, 904
AvaI CYCGRG 4 cut(s) 134, 560, 902, 1113
BaeGI GKGCMC 1 cut(s) 1350
BaeI ACNNNNGTAYC 2 cut(s) 1084, 1117
BalI TGGCCA 1 cut(s) 924
BbsI GAAGAC 1 cut(s) 824
Bbv12I GWGCWC 1 cut(s) 1350
BbvI GCAGC 4 cut(s) 61, 312, 340, 1091
BccI CCATC 5 cut(s) 260, 990, 1189, 1237, 1256
BceAI ACGGC 1 cut(s) 1113
BciT130I CCWGG 1 cut(s) 914
BclI TGATCA 1 cut(s) 1203
BcnI CCSGG 2 cut(s) 903, 904
BcoDI GTCTC 1 cut(s) 1067
BcuI ACTAGT 1 cut(s) 409
BfaI CTAG 5 cut(s) 410, 809, 1037, 1046, 1280
BfmI CTRYAG 1 cut(s) 326
BfoI RGCGCY 1 cut(s) 109
BfrI CTTAAG 1 cut(s) 482
BfuAI ACCTGC 1 cut(s) 1112
BglII AGATCT 2 cut(s) 295, 1248
BisI GCNGC 4 cut(s) 75, 326, 329, 1080
BlsI GCNGC 4 cut(s) 76, 327, 330, 1081
Bme1390I CCNGG 3 cut(s) 903, 904, 914
BmeT110I CYCGRG 4 cut(s) 134, 560, 902, 1113
BmgBI CACGTC 1 cut(s) 1034
BmgT120I GGNCC 2 cut(s) 452, 662
BmrFI CCNGG 3 cut(s) 903, 904, 914
BmsI GCATC 1 cut(s) 1148
BpiI GAAGAC 1 cut(s) 824
BpmI CTGGAG 1 cut(s) 896
BpuEI CTTGAG 3 cut(s) 254, 551, 611
BpuMI CCSGG 2 cut(s) 903, 904
BsaBI GATNNNNATC 2 cut(s) 294, 1247
BsaI GGTCTC 1 cut(s) 1067
BsaJI CCNNGG 3 cut(s) 37, 357, 902
BsaWI WCCGGW 1 cut(s) 281
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 170, 882
Bse118I RCCGGY 3 cut(s) 281, 943, 1009
Bse1I ACTGG 2 cut(s) 876, 1148
Bse3DI GCAATG 4 cut(s) 114, 945, 1011, 1336
Bse8I GATNNNNATC 2 cut(s) 294, 1247
BseBI CCWGG 1 cut(s) 914
BseDI CCNNGG 3 cut(s) 37, 357, 902
BseGI GGATG 1 cut(s) 440
BseJI GATNNNNATC 2 cut(s) 294, 1247
BseLI CCNNNNNNNGG 3 cut(s) 102, 170, 882
BseMI GCAATG 4 cut(s) 114, 945, 1011, 1336
BseMII CTCAG 2 cut(s) 516, 947
BseNI ACTGG 2 cut(s) 876, 1148
BseRI GAGGAG 1 cut(s) 1133
BseSI GKGCMC 1 cut(s) 1350
BseXI GCAGC 4 cut(s) 61, 312, 340, 1091
BsgI GTGCAG 3 cut(s) 540, 651, 872
Bsh1236I CGCG 1 cut(s) 333
Bsh1285I CGRYCG 1 cut(s) 609
BshFI GGCC 5 cut(s) 454, 470, 663, 924, 1128
BshTI ACCGGT 1 cut(s) 281
BsiEI CGRYCG 1 cut(s) 609
BsiHKAI GWGCWC 1 cut(s) 1350
BsiHKCI CYCGRG 4 cut(s) 134, 560, 902, 1113
BsiSI CCGG 4 cut(s) 282, 903, 944, 1010
BslFI GGGAC 4 cut(s) 110, 381, 919, 1106
BslI CCNNNNNNNGG 3 cut(s) 102, 170, 882
BsmAI GTCTC 1 cut(s) 1067
BsmFI GGGAC 4 cut(s) 110, 381, 919, 1106
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 5 cut(s) 454, 470, 663, 924, 1128
Bso31I GGTCTC 1 cut(s) 1067
BsoBI CYCGRG 4 cut(s) 134, 560, 902, 1113
Bsp1286I GDGCHC 1 cut(s) 1350
Bsp143I GATC 7 cut(s) 7, 31, 295, 349, 793, 1203, 1248
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 3 cut(s) 333, 1089, 1156
BspANI GGCC 5 cut(s) 454, 470, 663, 924, 1128
BspCNI CTCAG 2 cut(s) 515, 946
BspFNI CGCG 1 cut(s) 333
BspHI TCATGA 1 cut(s) 396
BspMAI CTGCAG 1 cut(s) 330
BspMI ACCTGC 1 cut(s) 1112
BspPI GGATC 1 cut(s) 15
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 1067
BsrDI GCAATG 4 cut(s) 114, 945, 1011, 1336
BsrFI RCCGGY 3 cut(s) 281, 943, 1009
BsrI ACTGG 2 cut(s) 876, 1148
BssAI RCCGGY 3 cut(s) 281, 943, 1009
BssECI CCNNGG 3 cut(s) 37, 357, 902
BssMI GATC 7 cut(s) 7, 31, 295, 349, 793, 1203, 1248
BssT1I CCWWGG 2 cut(s) 37, 357
Bst2UI CCWGG 1 cut(s) 914
Bst4CI ACNGT 5 cut(s) 610, 1073, 1223, 1275, 1352
BstAFI CTTAAG 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 1006
BstC8I GCNNGC 4 cut(s) 550, 622, 1002, 1137
BstDEI CTNAG 5 cut(s) 502, 760, 842, 933, 967
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 440
BstFNI CGCG 1 cut(s) 333
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 2 cut(s) 108, 333
BstKTI GATC 7 cut(s) 10, 34, 298, 352, 796, 1206, 1251
BstMAI GTCTC 1 cut(s) 1067
BstMBI GATC 7 cut(s) 7, 31, 295, 349, 793, 1203, 1248
BstMCI CGRYCG 1 cut(s) 609
BstMWI GCNNNNNNNGC 5 cut(s) 535, 554, 563, 1006, 1289
BstNI CCWGG 1 cut(s) 914
BstSCI CCNGG 3 cut(s) 901, 902, 912
BstSFI CTRYAG 1 cut(s) 326
BstSLI GKGCMC 1 cut(s) 1350
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 4 cut(s) 61, 312, 340, 1091
BstV2I GAAGAC 1 cut(s) 824
BstX2I RGATCY 3 cut(s) 7, 295, 1248
BstXI CCANNNNNNTGG 2 cut(s) 364, 920
BstYI RGATCY 3 cut(s) 7, 295, 1248
BsuRI GGCC 5 cut(s) 454, 470, 663, 924, 1128
BtgI CCRYGG 1 cut(s) 357
BtrI CACGTC 1 cut(s) 1034
BtsCI GGATG 1 cut(s) 440
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 5 cut(s) 314, 714, 883, 1069, 1155
BveI ACCTGC 1 cut(s) 1112
Cac8I GCNNGC 4 cut(s) 550, 622, 1002, 1137
CaiI CAGNNNCTG 1 cut(s) 665
CciI TCATGA 1 cut(s) 396
CfoI GCGC 2 cut(s) 108, 333
Cfr10I RCCGGY 3 cut(s) 281, 943, 1009
Cfr13I GGNCC 2 cut(s) 452, 662
Cfr9I CCCGGG 1 cut(s) 902
CseI GACGC 3 cut(s) 805, 1103, 1281
Csp6I GTAC 2 cut(s) 1067, 1101
CspAI ACCGGT 1 cut(s) 281
CviAII CATG 6 cut(s) 239, 358, 397, 587, 838, 985
CviQI GTAC 2 cut(s) 1067, 1101
DdeI CTNAG 5 cut(s) 502, 760, 842, 933, 967
DpnI GATC 7 cut(s) 9, 33, 297, 351, 795, 1205, 1250
DpnII GATC 7 cut(s) 7, 31, 295, 349, 793, 1203, 1248
EaeI YGGCCR 1 cut(s) 922
Eco130I CCWWGG 2 cut(s) 37, 357
Eco31I GGTCTC 1 cut(s) 1067
Eco88I CYCGRG 4 cut(s) 134, 560, 902, 1113
EcoO109I RGGNCCY 1 cut(s) 662
EcoRII CCWGG 1 cut(s) 912
EcoT14I CCWWGG 2 cut(s) 37, 357
ErhI CCWWGG 2 cut(s) 37, 357
FaeI CATG 6 cut(s) 242, 361, 400, 590, 841, 988
FalI AAGNNNNNCTT 2 cut(s) 878, 910
FaqI GGGAC 4 cut(s) 110, 381, 919, 1106
FatI CATG 6 cut(s) 238, 357, 396, 586, 837, 984
FauI CCCGC 1 cut(s) 1082
FbaI TGATCA 1 cut(s) 1203
FblI GTMKAC 1 cut(s) 22
Fnu4HI GCNGC 4 cut(s) 75, 326, 329, 1080
FokI GGATG 1 cut(s) 447
Fsp4HI GCNGC 4 cut(s) 75, 326, 329, 1080
FspBI CTAG 5 cut(s) 410, 809, 1037, 1046, 1280
GlaI GCGC 2 cut(s) 107, 332
GluI GCNGC 4 cut(s) 75, 326, 329, 1080
GsuI CTGGAG 1 cut(s) 896
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 5 cut(s) 454, 470, 663, 924, 1128
HapII CCGG 4 cut(s) 282, 903, 944, 1010
HgaI GACGC 3 cut(s) 805, 1103, 1281
HhaI GCGC 2 cut(s) 108, 333
Hin1II CATG 6 cut(s) 242, 361, 400, 590, 841, 988
Hin6I GCGC 2 cut(s) 106, 331
HinP1I GCGC 2 cut(s) 106, 331
HindIII AAGCTT 1 cut(s) 887
HpaII CCGG 4 cut(s) 282, 903, 944, 1010
Hpy166II GTNNAC 6 cut(s) 23, 176, 1031, 1069, 1271, 1348
Hpy188I TCNGA 8 cut(s) 159, 257, 303, 517, 600, 821, 885, 1297
Hpy188III TCNNGA 8 cut(s) 134, 397, 497, 628, 1018, 1037, 1115, 1169
Hpy8I GTNNAC 6 cut(s) 23, 176, 1031, 1069, 1271, 1348
Hpy99I CGWCG 1 cut(s) 1297
HpyAV CCTTC 3 cut(s) 164, 182, 1231
HpyCH4III ACNGT 5 cut(s) 610, 1073, 1223, 1275, 1352
HpyCH4IV ACGT 3 cut(s) 672, 1033, 1184
HpyF10VI GCNNNNNNNGC 5 cut(s) 535, 554, 563, 1006, 1289
HpyF3I CTNAG 5 cut(s) 502, 760, 842, 933, 967
HpySE526I ACGT 3 cut(s) 672, 1033, 1184
Hsp92II CATG 6 cut(s) 242, 361, 400, 590, 841, 988
HspAI GCGC 2 cut(s) 106, 331
Ksp22I TGATCA 1 cut(s) 1203
Kzo9I GATC 7 cut(s) 7, 31, 295, 349, 793, 1203, 1248
Lsp1109I GCAGC 4 cut(s) 61, 312, 340, 1091
LweI GCATC 1 cut(s) 1148
MaeI CTAG 5 cut(s) 410, 809, 1037, 1046, 1280
MaeII ACGT 3 cut(s) 672, 1033, 1184
MalI GATC 7 cut(s) 9, 33, 297, 351, 795, 1205, 1250
MboI GATC 7 cut(s) 7, 31, 295, 349, 793, 1203, 1248
MboII GAAGA 4 cut(s) 179, 290, 689, 824
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 3 cut(s) 7, 295, 1248
MhlI GDGCHC 1 cut(s) 1350
MlsI TGGCCA 1 cut(s) 924
MluCI AATT 8 cut(s) 260, 390, 422, 615, 639, 709, 748, 1024
MluNI TGGCCA 1 cut(s) 924
MlyI GAGTC 5 cut(s) 140, 481, 494, 625, 901
MmeI TCCRAC 1 cut(s) 1275
Mox20I TGGCCA 1 cut(s) 924
MscI TGGCCA 1 cut(s) 924
MseI TTAA 8 cut(s) 483, 569, 642, 707, 747, 788, 1053, 1257
MslI CAYNNNNRTG 1 cut(s) 271
Msp20I TGGCCA 1 cut(s) 924
MspA1I CMGCKG 1 cut(s) 1082
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 4 cut(s) 282, 903, 944, 1010
MspR9I CCNGG 3 cut(s) 903, 904, 914
MunI CAATTG 1 cut(s) 615
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 1 cut(s) 914
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 5 cut(s) 535, 554, 563, 1006, 1289
NciI CCSGG 2 cut(s) 903, 904
NcoI CCATGG 1 cut(s) 357
NdeII GATC 7 cut(s) 7, 31, 295, 349, 793, 1203, 1248
NlaIII CATG 6 cut(s) 242, 361, 400, 590, 841, 988
OliI CACNNNNGTG 1 cut(s) 271
PaeR7I CTCGAG 2 cut(s) 134, 560
PagI TCATGA 1 cut(s) 396
PctI GAATGC 1 cut(s) 607
PfeI GAWTC 5 cut(s) 245, 304, 694, 1165, 1298
PflFI GACNNNGTC 1 cut(s) 610
PflMI CCANNNNNTGG 1 cut(s) 102
PinAI ACCGGT 1 cut(s) 281
PkrI GCNGC 4 cut(s) 76, 327, 330, 1081
PleI GAGTC 5 cut(s) 140, 481, 494, 625, 901
PpsI GAGTC 5 cut(s) 140, 481, 494, 625, 901
PsiI TTATAA 1 cut(s) 1337
Psp1406I AACGTT 1 cut(s) 1184
Psp6I CCWGG 1 cut(s) 912
PspGI CCWGG 1 cut(s) 912
PspPI GGNCC 2 cut(s) 452, 662
PspXI VCTCGAGB 1 cut(s) 560
PstI CTGCAG 1 cut(s) 330
PstNI CAGNNNCTG 1 cut(s) 665
PsuI RGATCY 3 cut(s) 7, 295, 1248
PsyI GACNNNGTC 1 cut(s) 610
PvuII CAGCTG 1 cut(s) 1082
RsaI GTAC 2 cut(s) 1068, 1102
RsaNI GTAC 2 cut(s) 1067, 1101
RseI CAYNNNNRTG 1 cut(s) 271
SaqAI TTAA 8 cut(s) 483, 569, 642, 707, 747, 788, 1053, 1257
SatI GCNGC 4 cut(s) 75, 326, 329, 1080
Sau3AI GATC 7 cut(s) 7, 31, 295, 349, 793, 1203, 1248
Sau96I GGNCC 2 cut(s) 452, 662
SchI GAGTC 5 cut(s) 140, 481, 494, 625, 901
ScrFI CCNGG 3 cut(s) 903, 904, 914
SduI GDGCHC 1 cut(s) 1350
SfaNI GCATC 1 cut(s) 1148
SfcI CTRYAG 1 cut(s) 326
Sfr274I CTCGAG 2 cut(s) 134, 560
SlaI CTCGAG 2 cut(s) 134, 560
SmaI CCCGGG 1 cut(s) 904
SmiMI CAYNNNNRTG 1 cut(s) 271
SmlI CTYRAG 6 cut(s) 134, 269, 482, 530, 560, 626
SmoI CTYRAG 6 cut(s) 134, 269, 482, 530, 560, 626
SpeI ACTAGT 1 cut(s) 409
Sse9I AATT 8 cut(s) 260, 390, 422, 615, 639, 709, 748, 1024
SsiI CCGC 3 cut(s) 333, 1089, 1156
SspMI CTAG 5 cut(s) 410, 809, 1037, 1046, 1280
StyD4I CCNGG 3 cut(s) 901, 902, 912
StyI CCWWGG 2 cut(s) 37, 357
TaaI ACNGT 5 cut(s) 610, 1073, 1223, 1275, 1352
TaiI ACGT 3 cut(s) 675, 1036, 1187
TaqI TCGA 4 cut(s) 135, 196, 387, 561
TasI AATT 8 cut(s) 260, 390, 422, 615, 639, 709, 748, 1024
TfiI GAWTC 5 cut(s) 245, 304, 694, 1165, 1298
Tru1I TTAA 8 cut(s) 483, 569, 642, 707, 747, 788, 1053, 1257
Tru9I TTAA 8 cut(s) 483, 569, 642, 707, 747, 788, 1053, 1257
TscAI CASTG 5 cut(s) 314, 721, 883, 1076, 1155
TseI GCWGC 4 cut(s) 74, 325, 328, 1079
TspDTI ATGAA 2 cut(s) 373, 385
TspMI CCCGGG 1 cut(s) 902
TspRI CASTG 5 cut(s) 314, 721, 883, 1076, 1155
Tth111I GACNNNGTC 1 cut(s) 610
Van91I CCANNNNNTGG 1 cut(s) 102
Vha464I CTTAAG 1 cut(s) 482
VneI GTGCAC 1 cut(s) 1346
XapI RAATTY 2 cut(s) 260, 390
XbaI TCTAGA 1 cut(s) 1036
XhoI CTCGAG 2 cut(s) 134, 560
XmaI CCCGGG 1 cut(s) 902
XmiI GTMKAC 1 cut(s) 22
XspI CTAG 5 cut(s) 410, 809, 1037, 1046, 1280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.