AT3G08850

Regulatory-associated protein of TOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002483
Physical Location & Seq
Reverse (-)
2686457 .. 2695400
8944 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G08850.1

Sequence Viewer

Length: 4035 bp
ATGGCATTAGGAGACTTAATGGTGTCTCGGTTCTCGCAATCTTCTGTTTCTTTGGTCTCCAATCACCGTTACGACGAAGACTGTGTCTCTAGTCACGATGACGGTGATTCCCGGAGGAAGGATTCTGAGGCTAAGAGCAGTAGTAGCTACGGGAATGGTACAACGGAGGGAGCCGCCACCGCCACCAGCATGGCTTACTTGCCTCAGACTATTGTGCTCTGCGAGCTTAGACACGACGCATCCGAGGCTTCTGCTCCCTTGGGGACTTCTGAGATCGTATTGGTCCCCAAATGGCGGCTTAAAGAACGAATGAAAACAGGATGTGTAGCTTTAGTTCTGTGTTTAAACATTACTGTTGATCCGCCGGATGTTATAAAGATATCTCCTTGTGCTAGAATCGAGGCATGGATAGATCCATTTTCTATGGCACCGCCTAAAGCTCTCGAGACAATTGGAAAAAATTTGAGCACTCAGTATGAGAGATGGCAACCCAGGGCCCGCTATAAGGTTCAGCTTGATCCGACAGTAGATGAGGTGAGGAAGCTGTGCTTGACTTGTCGGAAATATGCAAAGACCGAGAGAGTTCTATTCCATTATAATGGGCATGGTGTGCCAAAACCTACAGCTAATGGTGAAATTTGGGTATTTAACAAGAGTTATACACAGTACATTCCCTTGCCAATCAGTGAACTTGATTCCTGGTTGAAGACACCCTCCATCTATGTTTTCGACTGCTCTGCTGCTAGGATGATTCTGAATGCCTTTGCTGAGCTTCATGATTGGGGTTCTTCTGGTTCCTCTGGATCCTCAAGGGACTGCATTCTACTTGCTGCTTGTGATGTACATGAGACACTTCCTCAGAGCGTTGAGTTTCCAGCTGATGTTTTTACGTCTTGCCTGACAACGCCCATTAAAATGGCGTTGAAATGGTTCTGCAGGCGTTCGCTTCTGAAAGAAATTATCGATGAATCACTTATCGACAGGATTCCAGGCCGGCAAAACGATCGTAAGACATTGTTAGGGGAGTTGAACTGGATTTTCACAGCAGTGACGGATACAATTGCTTGGAATGTGCTTCCTCATGAACTTTTCCAGAGATTATTCAGACAGGACTTGTTGGTCGCTAGCCTTTTCCGGAATTTCTTACTTGCTGAGAGAATAATGCGGTCCGCAAACTGTAATCCAATATCTCACCCTATGCTGCCTCCTACGCATCAACATCATATGTGGGATGCATGGGACATGGCTGCTGAAATCTGTCTTTCTCAGCTTCCCCAACTTGTTCTGGACCCAAGCACAGAGTTCCAGCCAAGTCCGTTTTTTACTGAGCAACTGACAGCTTTTGAGGTGTGGCTTGATCATGGATCTGAGCATAAGAAGCCACCGGAGCAGTTACCTATTGTTCTTCAGGTGTTACTTAGCCAGTGCCATCGGTTTCGTGCTCTTGTACTTCTTGGAAGATTTCTTGATATGGGTTCATGGGCTGTGGATCTGGCCTTGTCTGTTGGAATATTCCCATATGTGCTGAAGCTTCTGCAAACAACAACGAATGAGCTAAGACAGATCCTGGTTTTCATATGGACAAAAATTCTTGCACTTGACAAGTCATGTCAAATTGATCTTGTGAAGGATGGGGGGCATACATACTTCATACGATTTCTAGATAGCTCGGGTGCATTTCCAGAACAACGAGCTATGGCTGCTTTTGTTCTGGCTGTCATTGTGGATGGACATCGACGAGGCCAGGAAGCATGTCTTGAAGCTAATTTAATTGGTGTTTGTCTCGGGCACCTTGAAGCATCCAGACCAAGTGATCCACAACCAGAACCATTGTTTCTACAATGGCTTTGTCTTTGTCTTGGAAAGTTATGGGAAGATTTTATGGAGGCCCAAATAATGGGCAGGGAGGCAAATGCTTTTGAAAAGTTGGCACCTCTGCTTTCCGAGCCCCAACCTGAGGTAAGGGCTGCTGCAGTTTTTGCCCTGGGTACCTTGCTTGATATTGGGTTTGACTCTAATAAAAGTGTGGTGGAGGATGAATTTGATGATGATGAAAAGATTAGAGCCGAAGACGCTATCATTAAAAGTCTCTTAGATGTAGTTTCAGATGGGAGTCCACTTGTCCGAGCAGAGGTTGCCGTAGCTCTTGCACGTTTTGCCTTTGGCCACAAACAGCACCTTAAGTTAGCCGCAGCTTCATATTGGAAGCCTCAGTCAAGTTCTTTGCTTACTTCTCTACCTTCAATAGCTAAATTCCATGATCCTGGGAGTGCAACAATTGTTTCTTTACACATGAGTCCTCTGACTAGAGCTAGCACGGATAGCCAACCAGTGGCTCGTGAGTCTAGGATCTCAAGCAGCCCTCTTGGCTCCTCTGGGCTGATGCAAGGATCTCCATTATCTGATGATTCTTCGTTACATTCTGATTCTGGAATGATGCATGACAGTGTCAGCAATGGAGCGGTCCATCAGCCAAGACTGTTGGATAATGCTGTTTATTCGCAGTGCGTCCGAGCTATGTTTGCATTAGCTAAAGATCCATCTCCACGTATTGCAAGTCTCGGACGGCGTGTGCTTTCTATTATTGGAATCGAACAGGTTGTTGCGAAACCCTCGAAGCCCACTGGCCGACCAGGGGAAGCTGCAACGACATCTCACACTCCACTTGCTGGCCTAGCTCGTTCATCCTCATGGTTTGATATGCATGCAGGTAATCTGCCTTTAAGTTTTAGGACTCCCCCGGTCAGCCCCCCTAGAACAAACTACCTGAGTGGATTGAGGAGAGTTTGTTCATTAGAGTTCAGGCCTCATCTATTGGGTTCACCCGACTCAGGATTGGCTGATCCGCTTTTAGGCGCCAGTGGATCTGAACGGAGTTTGCTTCCACTATCAACTATCTACGGCTGGAGTTGTGGGCACTTTTCTAAGCCACTTCTTGGTGGTGCGGATGCTAGTCAAGAGATTGCAGCCAAAAGAGAAGAGAAAGAAAAATTCGCACTTGAGCATATTGCAAAATGCCAGCACTCTTCTATTAGCAAGCTCAACAATAATCCTATTGCCAACTGGGATACAAGGTTTGAAACGGGAACAAAGACAGCCCTCCTTCACCCATTCTCTCCTATTGTAGTTGCTGCAGACGAGAATGAACGGATCAGAGTGTGGAACTATGAGGAAGCAACTCTTCTCAATGGCTTTGACAATCATGATTTTCCTGACAAAGGAATTTCAAAGCTCTGCCTCATCAATGAACTTGACGACTCTCTGCTACTTGTTGCATCATGCGATGGGTCGGTCCGGATATGGAAAAACTATGCAACTAAGGGTAAACAAAAGCTTGTTACTGGGTTTTCTTCAATCCAGGGTCACAAGCCCGGTGCCCGTGACTTGAACGCTGTCGTGGACTGGCAACAACAGTCCGGTTACCTGTATGCTTCTGGGGAGACGTCAACAGTCACACTTTGGGACCTGGAGAAAGAACAGCTTGTCAGATCTGTTCCCTCTGAATCAGAATGTGGAGTTACAGCACTTTCTGCTTCTCAAGTGCACGGAGGCCAACTCGCTGCTGGTTTTGCTGATGGATCTTTGAGACTCTATGATGTTCGGTCACCTGAACCGCTTGTCTGCGCGACTCGGCCTCATCAGAAAGTTGAAAGGGTGGTTGGCCTCAGTTTTCAACCTGGACTTGACCCCGCAAAGGTGGTGAGTGCATCACAGGCGGGTGACATACAGTTTCTTGACCTTAGAACAACAAGGGACACATACCTGACGATTGATGCACACAGGGGTTCACTCACGGCCTTAGCTGTTCACAGACACGCTCCAATAATCGCGAGTGGATCTGCAAAACAGCTCATTAAAGTATTCAGTCTTCAAGGGGAACAACTAGGGATAATCCGCTACTACCCATCCTTCATGGCTCAAAAGATTGGATCAGTGAGTTGCCTCACATTTCATCCGTACCAGGTTCTGCTAGCAGCTGGAGCTGCTGACTCATTTGTCTCCATATACACCCACGACAACTCGCAAGCAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0001558 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008361 GO:0009267 GO:0009605 GO:0009719 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009893 GO:0009894 GO:0009987 GO:0009991 GO:0010033 GO:0010073 GO:0010074 GO:0010154 GO:0010243 GO:0010492 GO:0010506 GO:0010562 GO:0010604 GO:0016043 GO:0019220 GO:0019222 GO:0019827 GO:0022414 GO:0023052 GO:0030307 GO:0030674 GO:0031323 GO:0031325 GO:0031329 GO:0031399 GO:0031401 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032535 GO:0032991 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040008 GO:0042221 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043200 GO:0043549 GO:0044093 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045927 GO:0045937 GO:0048316 GO:0048507 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060090 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071840 GO:0071900 GO:0071902 GO:0080008 GO:0080090 GO:0090066 GO:0098727 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04714 ko04910 ko05206 map04136 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04714 map04910 map05206
Pfam Domains
Protein Families
HEAT Raptor_N WD40

Protein Analysis

1344

Amino Acids

147.96

Weight (kDa)

6.2

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raptor_N PF14538 104 - 255 7.8e-70 Raptor N-terminal CASPase like domain
WD40_WDHD1_1st PF24817 1014 - 1226 4.6e-09 WDHD1 first WD40 domain
WD40_Prp19 PF24814 1040 - 1251 5.3e-12 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 1042 - 1209 4.6e-11 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1085 - 1340 6.1e-19 THOC3 beta-propeller domain
WDR55 PF24796 1087 - 1290 3.6e-08 WDR55
WD40_MABP1-WDR62_2nd PF24782 1087 - 1337 5.2e-06 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 1093 - 1226 1e-07 CDC20/Fizzy WD40 domain
WD40_CDC20-Fz PF24807 1132 - 1335 4e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 1132 - 1292 1e-09 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 1141 - 1284 3.2e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 1144 - 1337 1.5e-15 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 1148 - 1287 1.2e-10 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 1219 - 1337 1.2e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 374, 597
AasI GACNNNNNNGTC 2 cut(s) 1118, 3995
AatII GACGTC 1 cut(s) 3446
Acc36I ACCTGC 1 cut(s) 2699
Acc65I GGTACC 1 cut(s) 1988
AccB1I GGYRCC 6 cut(s) 427, 1788, 1930, 1988, 2855, 3374
AccB7I CCANNNNNTGG 4 cut(s) 1897, 2332, 2669, 2936
AccBSI CCGCTC 1 cut(s) 2462
AccII CGCG 2 cut(s) 3626, 3830
AccIII TCCGGA 2 cut(s) 1134, 3294
AcoI YGGCCR 2 cut(s) 2164, 2626
AcsI RAATTY 8 cut(s) 460, 636, 1138, 1587, 2039, 2252, 2990, 3222
AcuI CTGAAG 2 cut(s) 1391, 1547
AcyI GRCGYC 2 cut(s) 2856, 3443
AfaI GTAC 6 cut(s) 160, 668, 843, 1449, 1990, 3959
AflII CTTAAG 1 cut(s) 2180
AjuI GAANNNNNNNTTGG 2 cut(s) 3642, 3674
AleI CACNNNNGTG 1 cut(s) 1046
AloI GAACNNNNNNTCC 2 cut(s) 2773, 2805
Alw21I GWGCWC 4 cut(s) 219, 470, 1444, 3546
Alw44I GTGCAC 1 cut(s) 3542
AlwNI CAGNNNCTG 2 cut(s) 1567, 3967
Ama87I CYCGRG 3 cut(s) 443, 1669, 1784
Aor13HI TCCGGA 2 cut(s) 1134, 3294
ApaI GGGCCC 1 cut(s) 499
ApaLI GTGCAC 1 cut(s) 3542
ApoI RAATTY 8 cut(s) 460, 636, 1138, 1587, 2039, 2252, 2990, 3222
ArsI GACNNNNNNTTYG 2 cut(s) 69, 101
Asp700I GAANNNNTTC 1 cut(s) 929
Asp718I GGTACC 1 cut(s) 1988
AspLEI GCGC 2 cut(s) 2858, 3626
AspS9I GGNCC 9 cut(s) 283, 495, 496, 1167, 1288, 1888, 2464, 3292, 3463
AsuC2I CCSGG 3 cut(s) 112, 2741, 3372
AsuNHI GCTAGC 3 cut(s) 1124, 2312, 3970
AvaI CYCGRG 3 cut(s) 443, 1669, 1784
AvaII GGWCC 6 cut(s) 283, 1167, 1288, 2464, 3292, 3463
AxyI CCTNAGG 1 cut(s) 1956
BaeGI GKGCMC 5 cut(s) 499, 1791, 2919, 3379, 3546
BalI TGGCCA 1 cut(s) 2166
BamHI GGATCC 1 cut(s) 803
BanI GGYRCC 6 cut(s) 427, 1788, 1930, 1988, 2855, 3374
BanII GRGCYC 2 cut(s) 499, 1950
BarI GAAGNNNNNNTAC 2 cut(s) 3852, 3884
BauI CACGAG 1 cut(s) 2337
BbsI GAAGAC 4 cut(s) 84, 713, 2076, 3860
Bbv12I GWGCWC 4 cut(s) 219, 470, 1444, 3546
BceAI ACGGC 4 cut(s) 2123, 2582, 2917, 3810
BcgI CGANNNNNNTGC 6 cut(s) 233, 267, 719, 753, 986, 1020
BciVI GTATCC 2 cut(s) 1048, 3061
BclI TGATCA 1 cut(s) 1357
BcnI CCSGG 3 cut(s) 112, 2741, 3372
BfmI CTRYAG 4 cut(s) 621, 934, 1971, 3132
BfoI RGCGCY 1 cut(s) 2859
BfrI CTTAAG 1 cut(s) 2180
BfuAI ACCTGC 1 cut(s) 2699
BfuI GTATCC 2 cut(s) 1048, 3061
BglI GCCNNNNNGGC 1 cut(s) 2367
BglII AGATCT 1 cut(s) 3488
BlpI GCTNAGC 1 cut(s) 768
Bme18I GGWCC 6 cut(s) 283, 1167, 1288, 2464, 3292, 3463
BmeT110I CYCGRG 3 cut(s) 443, 1669, 1784
BmgT120I GGNCC 9 cut(s) 283, 495, 496, 1167, 1288, 1888, 2464, 3292, 3463
BmrI ACTGGG 2 cut(s) 3073, 3351
BmtI GCTAGC 3 cut(s) 1128, 2316, 3974
BmuI ACTGGG 2 cut(s) 3073, 3351
BpiI GAAGAC 4 cut(s) 84, 713, 2076, 3860
BplI GAGNNNNNCTC 2 cut(s) 3540, 3572
BpmI CTGGAG 3 cut(s) 2926, 3488, 3999
Bpu10I CCTNAGC 1 cut(s) 3799
Bpu1102I GCTNAGC 1 cut(s) 768
BpuEI CTTGAG 4 cut(s) 793, 2338, 3020, 3522
BpuMI CCSGG 3 cut(s) 112, 2741, 3372
Bsa29I ATCGAT 1 cut(s) 963
BsaAI YACGTR 1 cut(s) 2549
BsaBI GATNNNNATC 1 cut(s) 1731
BsaHI GRCGYC 2 cut(s) 2856, 3443
BsaI GGTCTC 1 cut(s) 61
BsaWI WCCGGW 4 cut(s) 1134, 1384, 3294, 3416
BsaXI ACNNNNNCTCC 2 cut(s) 2773, 2803
Bse118I RCCGGY 1 cut(s) 993
Bse1I ACTGG 8 cut(s) 1037, 1423, 2330, 2629, 2859, 3068, 3346, 3407
Bse21I CCTNAGG 1 cut(s) 1956
Bse3DI GCAATG 1 cut(s) 2461
Bse8I GATNNNNATC 1 cut(s) 1731
BseAI TCCGGA 2 cut(s) 1134, 3294
BseCI ATCGAT 1 cut(s) 963
BseJI GATNNNNATC 1 cut(s) 1731
BseMI GCAATG 1 cut(s) 2461
BseNI ACTGG 8 cut(s) 1037, 1423, 2330, 2629, 2859, 3068, 3346, 3407
BseRI GAGGAG 2 cut(s) 2362, 2794
BseSI GKGCMC 5 cut(s) 499, 1791, 2919, 3379, 3546
Bsh1236I CGCG 2 cut(s) 3626, 3830
Bsh1285I CGRYCG 1 cut(s) 1006
BshNI GGYRCC 6 cut(s) 427, 1788, 1930, 1988, 2855, 3374
BshVI ATCGAT 1 cut(s) 963
BsiEI CGRYCG 1 cut(s) 1006
BsiHKAI GWGCWC 4 cut(s) 219, 470, 1444, 3546
BsiHKCI CYCGRG 3 cut(s) 443, 1669, 1784
BsiSI CCGG 9 cut(s) 112, 365, 994, 1135, 1385, 2741, 3295, 3372, 3417
BslFI GGGAC 6 cut(s) 269, 277, 827, 1253, 3476, 3767
BsmBI CGTCTC 1 cut(s) 3434
BsmFI GGGAC 6 cut(s) 269, 277, 827, 1253, 3476, 3767
BsmI GAATGC 2 cut(s) 763, 819
Bso31I GGTCTC 1 cut(s) 61
BsoBI CYCGRG 3 cut(s) 443, 1669, 1784
Bsp120I GGGCCC 1 cut(s) 495
Bsp1286I GDGCHC 9 cut(s) 219, 470, 499, 1444, 1791, 1950, 2919, 3379, 3546
Bsp13I TCCGGA 2 cut(s) 1134, 3294
Bsp1407I TGTACA 1 cut(s) 841
Bsp1720I GCTNAGC 1 cut(s) 768
Bsp68I TCGCGA 1 cut(s) 3830
BspDI ATCGAT 1 cut(s) 963
BspEI TCCGGA 2 cut(s) 1134, 3294
BspFNI CGCG 2 cut(s) 3626, 3830
BspHI TCATGA 3 cut(s) 775, 1081, 3202
BspMAI CTGCAG 3 cut(s) 938, 1975, 3136
BspMI ACCTGC 1 cut(s) 2699
BspOI GCTAGC 3 cut(s) 1128, 2316, 3974
BspT107I GGYRCC 6 cut(s) 427, 1788, 1930, 1988, 2855, 3374
BspTI CTTAAG 1 cut(s) 2180
BspTNI GGTCTC 1 cut(s) 61
BsrBI CCGCTC 1 cut(s) 2462
BsrDI GCAATG 1 cut(s) 2461
BsrFI RCCGGY 1 cut(s) 993
BsrGI TGTACA 1 cut(s) 841
BsrI ACTGG 8 cut(s) 1037, 1423, 2330, 2629, 2859, 3068, 3346, 3407
BssAI RCCGGY 1 cut(s) 993
BssNI GRCGYC 2 cut(s) 2856, 3443
BssSI CACGAG 1 cut(s) 2337
BssT1I CCWWGG 1 cut(s) 258
Bst2BI CACGAG 1 cut(s) 2337
Bst6I CTCTTC 3 cut(s) 2973, 3031, 3186
BstACI GRCGYC 2 cut(s) 2856, 3443
BstAFI CTTAAG 1 cut(s) 2180
BstAPI GCANNNNNTGC 5 cut(s) 610, 1979, 2135, 2156, 3530
BstAUI TGTACA 1 cut(s) 841
BstBAI YACGTR 1 cut(s) 2549
BstEII GGTNACC 2 cut(s) 3419, 3603
BstENI CCTNNNNNAGG 1 cut(s) 3216
BstFNI CGCG 2 cut(s) 3626, 3830
BstH2I RGCGCY 1 cut(s) 2859
BstHHI GCGC 2 cut(s) 2858, 3626
BstMCI CGRYCG 1 cut(s) 1006
BstNSI RCATGY 2 cut(s) 1755, 2708
BstPI GGTNACC 2 cut(s) 3419, 3603
BstSFI CTRYAG 4 cut(s) 621, 934, 1971, 3132
BstSLI GKGCMC 5 cut(s) 499, 1791, 2919, 3379, 3546
BstUI CGCG 2 cut(s) 3626, 3830
BstV2I GAAGAC 4 cut(s) 84, 713, 2076, 3860
BstXI CCANNNNNNTGG 4 cut(s) 190, 599, 916, 2264
Bsu15I ATCGAT 1 cut(s) 963
Bsu36I CCTNAGG 1 cut(s) 1956
BsuI GTATCC 2 cut(s) 1048, 3061
BsuTUI ATCGAT 1 cut(s) 963
BtgZI GCGATG 1 cut(s) 3297
BtsI GCAGTG 2 cut(s) 1053, 2510
BtsIMutI CAGTG 9 cut(s) 691, 1053, 1430, 2337, 2452, 2510, 2622, 2866, 3939
BtuMI TCGCGA 1 cut(s) 3830
BveI ACCTGC 1 cut(s) 2699
CaiI CAGNNNCTG 2 cut(s) 1567, 3967
CciI TCATGA 3 cut(s) 775, 1081, 3202
CfoI GCGC 2 cut(s) 2858, 3626
Cfr10I RCCGGY 1 cut(s) 993
Cfr13I GGNCC 9 cut(s) 283, 495, 496, 1167, 1288, 1888, 2464, 3292, 3463
ClaI ATCGAT 1 cut(s) 963
CpoI CGGWCCG 2 cut(s) 1167, 3292
CseI GACGC 3 cut(s) 245, 2081, 2497
CsiI ACCWGGT 1 cut(s) 3960
Csp6I GTAC 6 cut(s) 159, 667, 842, 1448, 1989, 3958
CspI CGGWCCG 2 cut(s) 1167, 3292
CviQI GTAC 6 cut(s) 159, 667, 842, 1448, 1989, 3958
DinI GGCGCC 1 cut(s) 2857
DraI TTTAAA 1 cut(s) 345
DrdI GACNNNNNNGTC 2 cut(s) 1118, 3995
DseDI GACNNNNNNGTC 2 cut(s) 1118, 3995
EaeI YGGCCR 2 cut(s) 2164, 2626
Eam1104I CTCTTC 3 cut(s) 2973, 3031, 3186
EarI CTCTTC 3 cut(s) 2973, 3031, 3186
EciI GGCGGA 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 258
Eco147I AGGCCT 1 cut(s) 2806
Eco24I GRGCYC 2 cut(s) 499, 1950
Eco31I GGTCTC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 381
Eco47I GGWCC 6 cut(s) 283, 1167, 1288, 2464, 3292, 3463
Eco57I CTGAAG 2 cut(s) 1391, 1547
Eco81I CCTNAGG 1 cut(s) 1956
Eco88I CYCGRG 3 cut(s) 443, 1669, 1784
Eco91I GGTNACC 2 cut(s) 3419, 3603
EcoNI CCTNNNNNAGG 1 cut(s) 3216
EcoO109I RGGNCCY 2 cut(s) 495, 3463
EcoO65I GGTNACC 2 cut(s) 3419, 3603
EcoRV GATATC 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 258
EcoT22I ATGCAT 3 cut(s) 1237, 2442, 2706
EcoT38I GRGCYC 2 cut(s) 499, 1950
EgeI GGCGCC 1 cut(s) 2857
EheI GGCGCC 1 cut(s) 2857
ErhI CCWWGG 1 cut(s) 258
Esp3I CGTCTC 1 cut(s) 3434
FalI AAGNNNNNCTT 8 cut(s) 533, 565, 1740, 1772, 3165, 3197, 3465, 3497
FaqI GGGAC 6 cut(s) 269, 277, 827, 1253, 3476, 3767
FauI CCCGC 3 cut(s) 506, 3697, 3709
FauNDI CATATG 3 cut(s) 1224, 1519, 1577
FbaI TGATCA 1 cut(s) 1357
FriOI GRGCYC 2 cut(s) 499, 1950
GlaI GCGC 2 cut(s) 2857, 3625
GsuI CTGGAG 3 cut(s) 2926, 3488, 3999
HaeII RGCGCY 1 cut(s) 2859
HapII CCGG 9 cut(s) 112, 365, 994, 1135, 1385, 2741, 3295, 3372, 3417
HgaI GACGC 3 cut(s) 245, 2081, 2497
HhaI GCGC 2 cut(s) 2858, 3626
Hin1I GRCGYC 2 cut(s) 2856, 3443
Hin6I GCGC 2 cut(s) 2856, 3624
HinP1I GCGC 2 cut(s) 2856, 3624
HincII GTYRAC 1 cut(s) 3447
HindII GTYRAC 1 cut(s) 3447
HindIII AAGCTT 2 cut(s) 1529, 3332
HpaII CCGG 9 cut(s) 112, 365, 994, 1135, 1385, 2741, 3295, 3372, 3417
Hpy166II GTNNAC 9 cut(s) 689, 2117, 2822, 3326, 3400, 3447, 3544, 3788, 3808
Hpy8I GTNNAC 9 cut(s) 689, 2117, 2822, 3326, 3400, 3447, 3544, 3788, 3808
Hpy99I CGWCG 3 cut(s) 77, 239, 1740
HpyAV CCTTC 5 cut(s) 112, 1621, 2250, 3113, 3919
HpyCH4IV ACGT 4 cut(s) 890, 2152, 2548, 3443
HpySE526I ACGT 4 cut(s) 890, 2152, 2548, 3443
Hsp92I GRCGYC 2 cut(s) 2856, 3443
HspAI GCGC 2 cut(s) 2856, 3624
KasI GGCGCC 1 cut(s) 2855
Kpn2I TCCGGA 2 cut(s) 1134, 3294
KpnI GGTACC 1 cut(s) 1992
KroI GCCGGC 1 cut(s) 993
KroNI GCCGGC 1 cut(s) 995
Ksp22I TGATCA 1 cut(s) 1357
LmnI GCTCC 7 cut(s) 170, 259, 1387, 2375, 2459, 3823, 3980
MabI ACCWGGT 1 cut(s) 3960
MaeII ACGT 4 cut(s) 890, 2152, 2548, 3443
MbiI CCGCTC 1 cut(s) 2462
MfeI CAATTG 3 cut(s) 450, 1059, 2277
MhlI GDGCHC 9 cut(s) 219, 470, 499, 1444, 1791, 1950, 2919, 3379, 3546
MlsI TGGCCA 1 cut(s) 2166
MluNI TGGCCA 1 cut(s) 2166
Mly113I GGCGCC 1 cut(s) 2856
MmeI TCCRAC 4 cut(s) 539, 545, 1486, 2463
Mox20I TGGCCA 1 cut(s) 2166
Mph1103I ATGCAT 3 cut(s) 1237, 2442, 2706
MroI TCCGGA 2 cut(s) 1134, 3294
MroNI GCCGGC 1 cut(s) 993
MroXI GAANNNNTTC 1 cut(s) 929
MscI TGGCCA 1 cut(s) 2166
MslI CAYNNNNRTG 6 cut(s) 188, 597, 914, 1046, 2445, 2689
Msp20I TGGCCA 1 cut(s) 2166
MspA1I CMGCKG 2 cut(s) 878, 3977
MspCI CTTAAG 1 cut(s) 2180
MspI CCGG 9 cut(s) 112, 365, 994, 1135, 1385, 2741, 3295, 3372, 3417
MssI GTTTAAAC 1 cut(s) 345
MunI CAATTG 3 cut(s) 450, 1059, 2277
Mva1269I GAATGC 2 cut(s) 763, 819
MvnI CGCG 2 cut(s) 3626, 3830
NaeI GCCGGC 1 cut(s) 995
NarI GGCGCC 1 cut(s) 2856
NciI CCSGG 3 cut(s) 112, 2741, 3372
NdeI CATATG 3 cut(s) 1224, 1519, 1577
NgoMIV GCCGGC 1 cut(s) 993
NheI GCTAGC 3 cut(s) 1124, 2312, 3970
NmeAIII GCCGAG 1 cut(s) 3610
NmuCI GTSAC 7 cut(s) 92, 1048, 3362, 3380, 3451, 3603, 3719
NruI TCGCGA 1 cut(s) 3830
NsiI ATGCAT 3 cut(s) 1237, 2442, 2706
NspI RCATGY 2 cut(s) 1755, 2708
OliI CACNNNNGTG 1 cut(s) 1046
PaeI GCATGC 1 cut(s) 2708
PaeR7I CTCGAG 1 cut(s) 443
PagI TCATGA 3 cut(s) 775, 1081, 3202
PasI CCCWGGG 2 cut(s) 492, 1984
PceI AGGCCT 1 cut(s) 2806
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 2 cut(s) 763, 819
PdiI GCCGGC 1 cut(s) 995
PdmI GAANNNNTTC 1 cut(s) 929
PflFI GACNNNGTC 2 cut(s) 83, 2447
PflMI CCANNNNNTGG 4 cut(s) 1897, 2332, 2669, 2936
PfoI TCCNGGA 1 cut(s) 110
Ple19I CGATCG 1 cut(s) 1006
PluTI GGCGCC 1 cut(s) 2859
PmeI GTTTAAAC 1 cut(s) 345
Ppu21I YACGTR 1 cut(s) 2549
PpuMI RGGWCCY 1 cut(s) 3463
PsiI TTATAA 2 cut(s) 374, 597
Psp5II RGGWCCY 1 cut(s) 3463
PspEI GGTNACC 2 cut(s) 3419, 3603
PspOMI GGGCCC 1 cut(s) 495
PspPI GGNCC 9 cut(s) 283, 495, 496, 1167, 1288, 1888, 2464, 3292, 3463
PspPPI RGGWCCY 1 cut(s) 3463
PsrI GAACNNNNNNTAC 2 cut(s) 318, 350
PstI CTGCAG 3 cut(s) 938, 1975, 3136
PstNI CAGNNNCTG 2 cut(s) 1567, 3967
PsyI GACNNNGTC 2 cut(s) 83, 2447
PvuI CGATCG 1 cut(s) 1006
PvuII CAGCTG 2 cut(s) 878, 3977
RruI TCGCGA 1 cut(s) 3830
RsaI GTAC 6 cut(s) 160, 668, 843, 1449, 1990, 3959
RsaNI GTAC 6 cut(s) 159, 667, 842, 1448, 1989, 3958
RseI CAYNNNNRTG 6 cut(s) 188, 597, 914, 1046, 2445, 2689
Rsr2I CGGWCCG 2 cut(s) 1167, 3292
RsrII CGGWCCG 2 cut(s) 1167, 3292
Sau96I GGNCC 9 cut(s) 283, 495, 496, 1167, 1288, 1888, 2464, 3292, 3463
SduI GDGCHC 9 cut(s) 219, 470, 499, 1444, 1791, 1950, 2919, 3379, 3546
SexAI ACCWGGT 1 cut(s) 3960
SfcI CTRYAG 4 cut(s) 621, 934, 1971, 3132
SfoI GGCGCC 1 cut(s) 2857
Sfr274I CTCGAG 1 cut(s) 443
SinI GGWCC 6 cut(s) 283, 1167, 1288, 2464, 3292, 3463
SlaI CTCGAG 1 cut(s) 443
SmiMI CAYNNNNRTG 6 cut(s) 188, 597, 914, 1046, 2445, 2689
SmlI CTYRAG 6 cut(s) 443, 808, 2180, 2353, 2999, 3537
SmoI CTYRAG 6 cut(s) 443, 808, 2180, 2353, 2999, 3537
SphI GCATGC 1 cut(s) 2708
SseBI AGGCCT 1 cut(s) 2806
SspDI GGCGCC 1 cut(s) 2855
SspI AATATT 1 cut(s) 1512
StuI AGGCCT 1 cut(s) 2806
StyI CCWWGG 1 cut(s) 258
TaiI ACGT 4 cut(s) 893, 2155, 2551, 3446
TaqI TCGA 8 cut(s) 399, 444, 729, 963, 978, 1735, 2592, 2615
TaqII GACCGA 3 cut(s) 590, 3280, 3591
TatI WGTACW 3 cut(s) 666, 841, 1447
TauI GCSGC 3 cut(s) 176, 298, 2192
TscAI CASTG 9 cut(s) 691, 1053, 1430, 2337, 2452, 2510, 2629, 2866, 3939
TseFI GTSAC 7 cut(s) 92, 1048, 3362, 3380, 3451, 3603, 3719
Tsp45I GTSAC 7 cut(s) 92, 1048, 3362, 3380, 3451, 3603, 3719
TspGWI ACGGA 8 cut(s) 179, 1067, 1305, 2333, 2887, 3163, 3561, 3945
TspRI CASTG 9 cut(s) 691, 1053, 1430, 2337, 2452, 2510, 2629, 2866, 3939
Tth111I GACNNNGTC 2 cut(s) 83, 2447
Van91I CCANNNNNTGG 4 cut(s) 1897, 2332, 2669, 2936
Vha464I CTTAAG 1 cut(s) 2180
VneI GTGCAC 1 cut(s) 3542
VpaK11BI GGWCC 6 cut(s) 283, 1167, 1288, 2464, 3292, 3463
XagI CCTNNNNNAGG 1 cut(s) 3216
XapI RAATTY 8 cut(s) 460, 636, 1138, 1587, 2039, 2252, 2990, 3222
XbaI TCTAGA 1 cut(s) 1660
XceI RCATGY 2 cut(s) 1755, 2708
XcmI CCANNNNNNNNNTGG 1 cut(s) 3560
XhoI CTCGAG 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 929
ZraI GACGTC 1 cut(s) 3444
Zsp2I ATGCAT 3 cut(s) 1237, 2442, 2706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.