Rh3BG111000

Regulatory-associated protein of TOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002483
Physical Location & Seq
Reverse (-)
9050855 .. 9061800
10946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG111000.1

Sequence Viewer

Length: 3690 bp
ATGGCTCCAACAAAAGCTCTTGAAACAATTGGTAAAACCTTGAGTACACAGTATGAAAGATGGCAACCAAGGGCTAAATACAGGGTTCAGCTTGATCCTACTATAGATGAAGTCAAGAAGCTTTGTAGTACATGCCGCAAATATGCCAAGTCTGAGAGAGTTCTTTTCCATTATAATGGTCATGGTGTACCCAAGCCAACTGCTAATGGTGAAATTTGGCTATTTAATAAGAGTTATACACAGTATATTCCCTTGCCAATTAGCGACCTTGATTCCTGGTTGAAGACACCTTCAATATATGTCTTTGACTGTTCTGCCGCTGGCATGATTGTTAATTCATTCGTTGAGCTCCATGATTGGGCTGGCTCTAACTCTGGATCTCCAAGAGATTGTATCCTGCTTGCTGCTTGTGAACCACACGAGACTCTTCCGCAGAGTGCGGAATTTCCTGCTGATGTCTTTACTTCTTGCCTCACAACTCCCATCAAGATGGCTTTAAGATGGTTCTGCACACGTTCCTTACTTCATGAGTCTCTTGATTATTCACTAATAGATAAAATCCCTGGCCGTCAAAATGACCGGAAAACCCTTTTGGGTGAATTGAACTGGATTTTTACTGCTATCACTGACACAATTGCATGGAATGTTCTTCCTCATGATCTTTTCCAGAGACTTTTCAGACAAGATTTGTTAGTTGCCAGCCTGTTTCGAAACTTTTTACTTGCCGAGCGGATTATGCGATCTGCAAATTGCTCTCCAATCTCTCATCCACAGTTGCCTTCTACCCATCAGCACCACATGTGGGATGCATGGGATATGGCTGCTGAGATATGCCTTTCTCAGCTTCCATTGTTGGTTGAGGATCCTAATGCTGAGTTTCAGCCAAGTCCATTTTTCACTGAGCAGTTGACTGCTTTTGAGGTATGGCTTGACCACGGGTCTGAACACAAGAAACCACCAGAGCAGTTGCCTATTGTTCTTCAGGTGTTACTTAGTCAATGCCATCGGTTTCGGGCTCTGGTTCTTCTCGGGAGATTCCTTGATATGGGACCCTGGGCTGTAGATCTGGCATTGTCTGTTGGAATATTTCCATATGTACTGAAGCTGTTGCAAACGATGACACCTGAACTACGACAGATTCTTGTATTCATTTGGACAAAAATTCTTGCACTGGATAAGTCATGCCAGGTGGATCTAGTGAAGGATGGGGGTCATATATATTTCATCAGGTTTCTTGATAGCATGGAAGCATATCCAGAGCAGCGTGCAATGGCTGCTTTTGTTTTGGCTGTAATAGTTGATGGCCATAGACGTGGCCAGGAAGCTTGTATTGAAGCAGGTTTAATCCATGTTTGTTTGAAGCACCTTCAGGGTCCAACACCAAATGACACACAAACTGAACCCCTATTTCTTCAGTGGCTTTGCCTCTGTCTGGGGAAACTATGGGAGGATTTCACAGAGGCCCAAATATTTGGTTTGCAGGCAGATGCCCCTGCAATATGTGCTCCTCTGCTTGCTGAGCCCCAACCAGAGGTGAGGGCTTCTGCTGTTTTCGCATTGGGCACCCTGCTTGATGTGGGTTCAGGATCATGTAGAGATGGCAATGGTGGAGACGATGAGTATGATGATGAGGAAAAGATAAGGGCTGAAATTAGTATCATTAAAAGCCTATTAAGTGTTGTTTCAGATGGAAGCCCTCTTGTCAGAGCAGAAGTTGCTGTTGCTCTAGGACGCTTTGCCTTTGGACACAACAAGCACCTGAAGTCGATTGCTGCTGCCTATTGGAAACCACAGTCTAATTCTCTTGTGACTTCCTTGCCTGCTTTGGTTAGTATTAAAGGTCCTGGTAGTGCTTCATCTCATATTGGTCCACTTTCGAGAGTTGGCAATGACAGCTCATTGGTTCGGGATGGAAGAGTTTCTACCAGCAGTCCCCTAGCAAGTTCTGGAATTATGCATGGATCTCCATTGTCTGATGATTTATCTCATCATTCTGATTCTGGAATATTAGATGATGGTGGGAGCAATGGTATTGTTAACCATTTGACTCCTAGACCCCTGGACAGTCAAATGTATTCTCAGTGTGTATTGGCTATGTGTAACTTAGCCAAAGATCCATCTCCACGAATTGCAAAAATTGGTCGCCGAATATTAGCCATTATAGGGATTGAGCAAGTGGTGGCGAAACCTGTAAAGTCCACGGGTAACAGTTTTCGACCTAGTGAATCCATTACAGCGTCTCCAAGTCAGAGTCTTGCTGCTGGATTGGCTCGATCGTCTTCATGGTTCGATATGAATGGAGGTCATTTACCTTTCCGAACTCCACCTGTTAGCCCTCCAAGGGCAAATTACTTGGCTGGAATGCGAAGGGTTTGCTCTCTAGAATTTAGGCCTCATCTAATGAGTCCAGATTCAGGTTTAGCTGATCCCCTTTTAGGTTCCGGGGGAACTTTTGGTGCCTCAGAACGAAGTTTTCTTCCTCAATCAACAATCTACAATTGGAGTTGCGGTCACTTTTCCAAGCCACTTCTTATAGGGGCAGATGACAGTAAAGAAATATTAACTAGAAGAGAGGAGAGAGAAAACTTTGCCATGGAGCACATAGCTAAATGCCGGCACTCATCTGTTAGCAAGCTTAATAATCAAATTGCAAGCTGGGACACAAAATTTGAAACGGGTACGAAAAGCATTTTGCTGGAACCATTCTCTCCTATTGTGATTGCGGCTGATGAGAATGAACGAATCAGGGTATGGAATTACCAAGAGGCTAAGGAGGCTATCCTTCTGAACAGCTTTGATAACCATGAATTTCCTGACAAAGGAATCTCCAAGCTTTGCTTTGTGAATGAACTTGATGACAGCCTGCTTCTTGCTGCCTCAAGTGATGGAAACATTCGGATTTGGAAAGATTATACTTTGAAGGGTAAACAGAAACTTGTTACTGCATTTTCTTCAATTCAAGGACATAAACCTGGTGTTCGAAGTCTTAATGCTGTTGTGGATTGGCAGCAGCAATCTGGATATCTGTATGCCTCTGGTGAGCTATCATCCATTATGCTTTGGGATCTCGATAAGGAGCAGCTTGTTAATTCTATTCATTCTCAATCAGACTGTAGCATTTCAGCATTGTCTGCATCTCAAGTTCATGGAAGTCAACTCACAGCTGGTTTTGTTGACGGTTCTGTTAGGCTCTATGATGTGCGAACTCCTGACATGCTTGTCTGTGAAATGCGGCCGCACACTCCAAAGGTTGTAGGAAACGCACAGAAGATAGAAAGAGTTGTTGGGAACACTCAGAAGGTAGAAAGAGTTGTGGGGATTGGCTTTCAACCGGGGCTTGATCCTTCTAAGATTGTAAGTGCATGTCAGGCTGGTGACATTCAGTTCCTTGATATCAGAAATAGTAAAGATCCCTACCTCACAATCGAAGCTCACAGGGGTTCACTCACAGCTTTAGCTGTTCATAGACACGCTCCGCTCATTGCCAGTGGCTCAGCCAAGCAGCTCATAAAAGTCTTTAGTTTGGAGGGCGAACAATTAGGCACCATTAGATACTACCCTTCCTTTATGGCCCAAAAAATTGGCCCTGTTAGCTGCCTAGCCTTCCATCCGTATGAAGTACTGCTTGCTGCTGGTGCTGCTGATGCTTGCGCGTCCATCTATGCCGATGACAACTCTCAAGGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0001558 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008361 GO:0009267 GO:0009605 GO:0009719 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009893 GO:0009894 GO:0009987 GO:0009991 GO:0010033 GO:0010073 GO:0010074 GO:0010154 GO:0010243 GO:0010492 GO:0010506 GO:0010562 GO:0010604 GO:0016043 GO:0019220 GO:0019222 GO:0019827 GO:0022414 GO:0023052 GO:0030307 GO:0030674 GO:0031323 GO:0031325 GO:0031329 GO:0031399 GO:0031401 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032535 GO:0032991 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040008 GO:0042221 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043200 GO:0043549 GO:0044093 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045927 GO:0045937 GO:0048316 GO:0048507 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060090 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071840 GO:0071900 GO:0071902 GO:0080008 GO:0080090 GO:0090066 GO:0098727 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04714 ko04910 ko05206 map04136 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04714 map04910 map05206
Pfam Domains
Protein Families
HEAT Raptor_N WD40

Protein Analysis

1229

Amino Acids

135.41

Weight (kDa)

6.19

Isoelectric Point (pI)

44.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raptor_N PF14538 3 - 114 3.1e-51 Raptor N-terminal CASPase like domain
WD40_WDHD1_1st PF24817 888 - 1070 1.1e-06 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 900 - 1173 1.7e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 904 - 1073 1.9e-12 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 906 - 1095 2.7e-07 TEP-1 second beta-propeller
WD40_Prp19 PF24814 908 - 1081 6.6e-10 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 998 - 1086 6.5e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 1010 - 1221 2.5e-09 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 1115 - 1220 5.5e-06 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 174
AasI GACNNNNNNGTC 1 cut(s) 3218
Acc36I ACCTGC 1 cut(s) 1328
AccB1I GGYRCC 3 cut(s) 1562, 2456, 3542
AccBSI CCGCTC 2 cut(s) 730, 3478
AccII CGCG 1 cut(s) 3653
AcoI YGGCCR 4 cut(s) 565, 1303, 1315, 3233
AcsI RAATTY 6 cut(s) 213, 443, 1161, 2384, 2666, 2807
AcuI CTGAAG 5 cut(s) 965, 1121, 1352, 1397, 1781
AfaI GTAC 6 cut(s) 46, 130, 189, 1098, 2680, 3621
AflIII ACRYGT 2 cut(s) 512, 798
AhdI GACNNNNNGTC 1 cut(s) 937
AjiI CACGTC 1 cut(s) 1313
AjnI CCWGG 8 cut(s) 275, 562, 1052, 1185, 1317, 1843, 2058, 2971
AjuI GAANNNNNNNTTGG 4 cut(s) 575, 607, 3267, 3299
Alw21I GWGCWC 3 cut(s) 351, 1507, 2601
Alw26I GTCTC 5 cut(s) 416, 537, 664, 1605, 2244
Ama87I CYCGRG 1 cut(s) 1028
AoxI GGCC 8 cut(s) 565, 1303, 1315, 1461, 2390, 3233, 3570, 3583
ApoI RAATTY 6 cut(s) 213, 443, 1161, 2384, 2666, 2807
ArsI GACNNNNNNTTYG 2 cut(s) 2651, 2683
Asp700I GAANNNNTTC 1 cut(s) 1918
AspLEI GCGC 1 cut(s) 3653
AspS9I GGNCC 7 cut(s) 1049, 1373, 1462, 1841, 1868, 3571, 3584
AsuC2I CCSGG 2 cut(s) 2443, 3333
AsuHPI GGTGA 5 cut(s) 221, 608, 1546, 3050, 3386
AsuII TTCGAA 2 cut(s) 709, 2980
AvaI CYCGRG 1 cut(s) 1028
AvaII GGWCC 4 cut(s) 1049, 1373, 1841, 1868
BaeGI GKGCMC 1 cut(s) 1565
BalI TGGCCA 2 cut(s) 1305, 1317
BamHI GGATCC 1 cut(s) 862
BanI GGYRCC 3 cut(s) 1562, 2456, 3542
BanII GRGCYC 3 cut(s) 351, 1018, 1524
BarI GAAGNNNNNNTAC 2 cut(s) 1906, 1938
BauI CACGAG 1 cut(s) 419
BbsI GAAGAC 2 cut(s) 290, 2271
Bbv12I GWGCWC 3 cut(s) 351, 1507, 2601
BceAI ACGGC 1 cut(s) 552
BcgI CGANNNNNNTGC 2 cut(s) 2355, 2389
BciT130I CCWGG 8 cut(s) 277, 564, 1054, 1187, 1319, 1845, 2060, 2973
BciVI GTATCC 1 cut(s) 404
BcnI CCSGG 2 cut(s) 2443, 3333
BcoDI GTCTC 5 cut(s) 416, 537, 664, 1605, 2244
BfaI CTAG 8 cut(s) 1196, 1727, 1937, 2052, 2220, 2381, 2565, 3599
BfmI CTRYAG 3 cut(s) 102, 1059, 3112
BfuAI ACCTGC 1 cut(s) 1328
BfuI GTATCC 1 cut(s) 404
BglII AGATCT 1 cut(s) 1063
BlpI GCTNAGC 2 cut(s) 1518, 3493
BmcAI AGTACT 1 cut(s) 3621
Bme18I GGWCC 4 cut(s) 1049, 1373, 1841, 1868
BmeRI GACNNNNNGTC 1 cut(s) 937
BmeT110I CYCGRG 1 cut(s) 1028
BmgBI CACGTC 1 cut(s) 1313
BmgT120I GGNCC 7 cut(s) 1049, 1373, 1462, 1841, 1868, 3571, 3584
BmsI GCATC 4 cut(s) 796, 1477, 3143, 3634
BpiI GAAGAC 2 cut(s) 290, 2271
Bpu10I CCTNAGC 1 cut(s) 2769
Bpu1102I GCTNAGC 2 cut(s) 1518, 3493
Bpu14I TTCGAA 2 cut(s) 709, 2980
BpuEI CTTGAG 4 cut(s) 61, 2863, 3123, 3663
BpuMI CCSGG 2 cut(s) 2443, 3333
BsaWI WCCGGW 1 cut(s) 579
BsaXI ACNNNNNCTCC 6 cut(s) 2014, 2044, 2224, 2254, 2494, 2524
Bse118I RCCGGY 1 cut(s) 2613
Bse1I ACTGG 3 cut(s) 611, 1176, 3486
Bse3DI GCAATG 5 cut(s) 1275, 1609, 1894, 2032, 3480
BseBI CCWGG 8 cut(s) 277, 564, 1054, 1187, 1319, 1845, 2060, 2973
BseGI GGATG 6 cut(s) 766, 811, 1210, 1915, 3047, 3607
BseMI GCAATG 5 cut(s) 1275, 1609, 1894, 2032, 3480
BseNI ACTGG 3 cut(s) 611, 1176, 3486
BseRI GAGGAG 2 cut(s) 1497, 2588
BseSI GKGCMC 1 cut(s) 1565
BseX3I CGGCCG 1 cut(s) 3233
BseYI CCCAGC 1 cut(s) 2655
BsgI GTGCAG 1 cut(s) 493
Bsh1236I CGCG 1 cut(s) 3653
Bsh1285I CGRYCG 2 cut(s) 2276, 3236
BshFI GGCC 8 cut(s) 567, 1305, 1317, 1463, 2392, 3235, 3572, 3585
BshNI GGYRCC 3 cut(s) 1562, 2456, 3542
BsiEI CGRYCG 2 cut(s) 2276, 3236
BsiHKAI GWGCWC 3 cut(s) 351, 1507, 2601
BsiHKCI CYCGRG 1 cut(s) 1028
BsiSI CCGG 4 cut(s) 580, 2442, 2614, 3332
BslFI GGGAC 3 cut(s) 1062, 1917, 2672
BsmAI GTCTC 5 cut(s) 416, 537, 664, 1605, 2244
BsmBI CGTCTC 2 cut(s) 1605, 2244
BsmFI GGGAC 3 cut(s) 1062, 1917, 2672
BsmI GAATGC 1 cut(s) 2367
BsnI GGCC 8 cut(s) 567, 1305, 1317, 1463, 2392, 3235, 3572, 3585
BsoBI CYCGRG 1 cut(s) 1028
Bsp119I TTCGAA 2 cut(s) 709, 2980
Bsp1286I GDGCHC 6 cut(s) 351, 1018, 1507, 1524, 1565, 2601
Bsp1720I GCTNAGC 2 cut(s) 1518, 3493
Bsp19I CCATGG 1 cut(s) 2592
BspANI GGCC 8 cut(s) 567, 1305, 1317, 1463, 2392, 3235, 3572, 3585
BspFNI CGCG 1 cut(s) 3653
BspHI TCATGA 2 cut(s) 526, 655
BspMI ACCTGC 1 cut(s) 1328
BspT104I TTCGAA 2 cut(s) 709, 2980
BspT107I GGYRCC 3 cut(s) 1562, 2456, 3542
BsrBI CCGCTC 2 cut(s) 730, 3478
BsrDI GCAATG 5 cut(s) 1275, 1609, 1894, 2032, 3480
BsrFI RCCGGY 1 cut(s) 2613
BsrI ACTGG 3 cut(s) 611, 1176, 3486
BssAI RCCGGY 1 cut(s) 2613
BssSI CACGAG 1 cut(s) 419
BssT1I CCWWGG 3 cut(s) 68, 2339, 2592
Bst2BI CACGAG 1 cut(s) 419
Bst2UI CCWGG 8 cut(s) 277, 564, 1054, 1187, 1319, 1845, 2060, 2973
Bst6I CTCTTC 3 cut(s) 432, 1909, 2563
BstAPI GCANNNNNTGC 4 cut(s) 1274, 1502, 1715, 3131
BstBI TTCGAA 2 cut(s) 709, 2980
BstDSI CCRYGG 3 cut(s) 934, 2199, 2592
BstENI CCTNNNNNAGG 1 cut(s) 2817
BstF5I GGATG 6 cut(s) 766, 811, 1210, 1915, 3047, 3607
BstFNI CGCG 1 cut(s) 3653
BstHHI GCGC 1 cut(s) 3653
BstMAI GTCTC 5 cut(s) 416, 537, 664, 1605, 2244
BstMCI CGRYCG 2 cut(s) 2276, 3236
BstNI CCWGG 8 cut(s) 277, 564, 1054, 1187, 1319, 1845, 2060, 2973
BstNSI RCATGY 4 cut(s) 135, 802, 3217, 3366
BstSFI CTRYAG 3 cut(s) 102, 1059, 3112
BstSLI GKGCMC 1 cut(s) 1565
BstUI CGCG 1 cut(s) 3653
BstV2I GAAGAC 2 cut(s) 290, 2271
BstX2I RGATCY 8 cut(s) 377, 862, 1063, 1192, 1961, 2113, 3064, 3409
BstXI CCANNNNNNTGG 5 cut(s) 176, 490, 1313, 1472, 3581
BstYI RGATCY 8 cut(s) 377, 862, 1063, 1192, 1961, 2113, 3064, 3409
BstZI CGGCCG 1 cut(s) 3233
BsuI GTATCC 1 cut(s) 404
BsuRI GGCC 8 cut(s) 567, 1305, 1317, 1463, 2392, 3235, 3572, 3585
BtgI CCRYGG 3 cut(s) 934, 2199, 2592
BtrI CACGTC 1 cut(s) 1313
BtsCI GGATG 6 cut(s) 766, 811, 1210, 1915, 3047, 3607
BtsIMutI CAGTG 6 cut(s) 624, 897, 1169, 1421, 2087, 3493
BveI ACCTGC 1 cut(s) 1328
CciI TCATGA 2 cut(s) 526, 655
CciNI GCGGCCGC 1 cut(s) 3233
CfoI GCGC 1 cut(s) 3653
Cfr10I RCCGGY 1 cut(s) 2613
Cfr13I GGNCC 7 cut(s) 1049, 1373, 1462, 1841, 1868, 3571, 3584
CseI GACGC 3 cut(s) 1740, 2226, 3642
CsiI ACCWGGT 1 cut(s) 2971
Csp6I GTAC 6 cut(s) 45, 129, 188, 1097, 2679, 3620
CviQI GTAC 6 cut(s) 45, 129, 188, 1097, 2679, 3620
DrdI GACNNNNNNGTC 1 cut(s) 3218
DriI GACNNNNNGTC 1 cut(s) 937
DseDI GACNNNNNNGTC 1 cut(s) 3218
EaeI YGGCCR 4 cut(s) 565, 1303, 1315, 3233
EagI CGGCCG 1 cut(s) 3233
Eam1104I CTCTTC 3 cut(s) 432, 1909, 2563
Eam1105I GACNNNNNGTC 1 cut(s) 937
EarI CTCTTC 3 cut(s) 432, 1909, 2563
Ecl136II GAGCTC 1 cut(s) 349
EclXI CGGCCG 1 cut(s) 3233
Eco130I CCWWGG 3 cut(s) 68, 2339, 2592
Eco147I AGGCCT 1 cut(s) 2392
Eco24I GRGCYC 3 cut(s) 351, 1018, 1524
Eco32I GATATC 2 cut(s) 3023, 3394
Eco47I GGWCC 4 cut(s) 1049, 1373, 1841, 1868
Eco52I CGGCCG 1 cut(s) 3233
Eco53kI GAGCTC 1 cut(s) 349
Eco57I CTGAAG 5 cut(s) 965, 1121, 1352, 1397, 1781
Eco88I CYCGRG 1 cut(s) 1028
EcoICRI GAGCTC 1 cut(s) 349
EcoNI CCTNNNNNAGG 1 cut(s) 2817
EcoO109I RGGNCCY 2 cut(s) 1049, 1841
EcoRII CCWGG 8 cut(s) 275, 562, 1052, 1185, 1317, 1843, 2058, 2971
EcoRV GATATC 2 cut(s) 3023, 3394
EcoT14I CCWWGG 3 cut(s) 68, 2339, 2592
EcoT22I ATGCAT 2 cut(s) 811, 1959
EcoT38I GRGCYC 3 cut(s) 351, 1018, 1524
ErhI CCWWGG 3 cut(s) 68, 2339, 2592
Esp3I CGTCTC 2 cut(s) 1605, 2244
FalI AAGNNNNNCTT 4 cut(s) 2822, 2854, 3609, 3641
FaqI GGGAC 3 cut(s) 1062, 1917, 2672
FauNDI CATATG 1 cut(s) 1093
FokI GGATG 6 cut(s) 753, 818, 1217, 1922, 3034, 3594
FriOI GRGCYC 3 cut(s) 351, 1018, 1524
FspBI CTAG 8 cut(s) 1196, 1727, 1937, 2052, 2220, 2381, 2565, 3599
GlaI GCGC 1 cut(s) 3652
GsaI CCCAGC 1 cut(s) 2659
HaeIII GGCC 8 cut(s) 567, 1305, 1317, 1463, 2392, 3235, 3572, 3585
HapII CCGG 4 cut(s) 580, 2442, 2614, 3332
HgaI GACGC 3 cut(s) 1740, 2226, 3642
HhaI GCGC 1 cut(s) 3653
Hin6I GCGC 1 cut(s) 3651
HinP1I GCGC 1 cut(s) 3651
HincII GTYRAC 4 cut(s) 909, 2038, 3155, 3175
HindII GTYRAC 4 cut(s) 909, 2038, 3155, 3175
HindIII AAGCTT 4 cut(s) 119, 1323, 2633, 2831
HpaI GTTAAC 1 cut(s) 2038
HpaII CCGG 4 cut(s) 580, 2442, 2614, 3332
HphI GGTGA 5 cut(s) 221, 608, 1546, 3050, 3386
HpyCH4IV ACGT 2 cut(s) 514, 1312
HpySE526I ACGT 2 cut(s) 514, 1312
HspAI GCGC 1 cut(s) 3651
KflI GGGWCCC 1 cut(s) 1049
KroI GCCGGC 1 cut(s) 2613
KroNI GCCGGC 1 cut(s) 2615
KspAI GTTAAC 1 cut(s) 2038
LmnI GCTCC 7 cut(s) 10, 354, 1510, 2022, 2596, 3076, 3478
LweI GCATC 4 cut(s) 796, 1477, 3143, 3634
MabI ACCWGGT 1 cut(s) 2971
MaeI CTAG 8 cut(s) 1196, 1727, 1937, 2052, 2220, 2381, 2565, 3599
MaeII ACGT 2 cut(s) 514, 1312
MaeIII GTNAC 7 cut(s) 987, 1807, 2099, 2204, 2510, 2938, 3374
MbiI CCGCTC 2 cut(s) 730, 3478
MfeI CAATTG 3 cut(s) 27, 633, 2497
MflI RGATCY 8 cut(s) 377, 862, 1063, 1192, 1961, 2113, 3064, 3409
MhlI GDGCHC 6 cut(s) 351, 1018, 1507, 1524, 1565, 2601
MlsI TGGCCA 2 cut(s) 1305, 1317
MluNI TGGCCA 2 cut(s) 1305, 1317
MlyI GAGTC 5 cut(s) 418, 539, 2041, 2260, 2413
MmeI TCCRAC 3 cut(s) 32, 1060, 1400
Mox20I TGGCCA 2 cut(s) 1305, 1317
Mph1103I ATGCAT 2 cut(s) 811, 1959
MroNI GCCGGC 1 cut(s) 2613
MroXI GAANNNNTTC 1 cut(s) 1918
MscI TGGCCA 2 cut(s) 1305, 1317
MslI CAYNNNNRTG 4 cut(s) 174, 488, 1311, 3612
Msp20I TGGCCA 2 cut(s) 1305, 1317
MspA1I CMGCKG 2 cut(s) 320, 3164
MspI CCGG 4 cut(s) 580, 2442, 2614, 3332
MunI CAATTG 3 cut(s) 27, 633, 2497
Mva1269I GAATGC 1 cut(s) 2367
MvaI CCWGG 8 cut(s) 277, 564, 1054, 1187, 1319, 1845, 2060, 2973
MvnI CGCG 1 cut(s) 3653
NaeI GCCGGC 1 cut(s) 2615
NciI CCSGG 2 cut(s) 2443, 3333
NcoI CCATGG 1 cut(s) 2592
NdeI CATATG 1 cut(s) 1093
NgoMIV GCCGGC 1 cut(s) 2613
NmeAIII GCCGAG 1 cut(s) 751
NmuCI GTSAC 3 cut(s) 1807, 2510, 3374
NotI GCGGCCGC 1 cut(s) 3233
NsiI ATGCAT 2 cut(s) 811, 1959
NspI RCATGY 4 cut(s) 135, 802, 3217, 3366
NspV TTCGAA 2 cut(s) 709, 2980
PagI TCATGA 2 cut(s) 526, 655
PasI CCCWGGG 1 cut(s) 1053
PceI AGGCCT 1 cut(s) 2392
PciI ACATGT 1 cut(s) 798
PctI GAATGC 1 cut(s) 2367
PdiI GCCGGC 1 cut(s) 2615
PdmI GAANNNNTTC 1 cut(s) 1918
PfeI GAWTC 8 cut(s) 272, 1035, 1138, 1997, 2225, 2411, 2742, 2823
Ple19I CGATCG 1 cut(s) 2276
PleI GAGTC 5 cut(s) 418, 538, 2041, 2259, 2412
PpsI GAGTC 5 cut(s) 418, 538, 2041, 2259, 2412
PpuMI RGGWCCY 2 cut(s) 1049, 1841
PscI ACATGT 1 cut(s) 798
PsiI TTATAA 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 351
Psp5II RGGWCCY 2 cut(s) 1049, 1841
Psp6I CCWGG 8 cut(s) 275, 562, 1052, 1185, 1317, 1843, 2058, 2971
PspFI CCCAGC 1 cut(s) 2655
PspGI CCWGG 8 cut(s) 275, 562, 1052, 1185, 1317, 1843, 2058, 2971
PspPI GGNCC 7 cut(s) 1049, 1373, 1462, 1841, 1868, 3571, 3584
PspPPI RGGWCCY 2 cut(s) 1049, 1841
PsuI RGATCY 8 cut(s) 377, 862, 1063, 1192, 1961, 2113, 3064, 3409
PvuI CGATCG 1 cut(s) 2276
PvuII CAGCTG 1 cut(s) 3164
RsaI GTAC 6 cut(s) 46, 130, 189, 1098, 2680, 3621
RsaNI GTAC 6 cut(s) 45, 129, 188, 1097, 2679, 3620
RseI CAYNNNNRTG 4 cut(s) 174, 488, 1311, 3612
SacI GAGCTC 1 cut(s) 351
Sau96I GGNCC 7 cut(s) 1049, 1373, 1462, 1841, 1868, 3571, 3584
ScaI AGTACT 1 cut(s) 3621
SchI GAGTC 5 cut(s) 418, 539, 2041, 2260, 2413
SduI GDGCHC 6 cut(s) 351, 1018, 1507, 1524, 1565, 2601
SexAI ACCWGGT 1 cut(s) 2971
SfaNI GCATC 4 cut(s) 796, 1477, 3143, 3634
SfcI CTRYAG 3 cut(s) 102, 1059, 3112
SfuI TTCGAA 2 cut(s) 709, 2980
SinI GGWCC 4 cut(s) 1049, 1373, 1841, 1868
SmiMI CAYNNNNRTG 4 cut(s) 174, 488, 1311, 3612
SmlI CTYRAG 4 cut(s) 40, 2878, 3138, 3678
SmoI CTYRAG 4 cut(s) 40, 2878, 3138, 3678
SseBI AGGCCT 1 cut(s) 2392
SspI AATATT 5 cut(s) 1086, 1470, 2007, 2151, 2559
SspMI CTAG 8 cut(s) 1196, 1727, 1937, 2052, 2220, 2381, 2565, 3599
SstI GAGCTC 1 cut(s) 351
StuI AGGCCT 1 cut(s) 2392
StyI CCWWGG 3 cut(s) 68, 2339, 2592
TaiI ACGT 2 cut(s) 517, 1315
TaqI TCGA 9 cut(s) 709, 1766, 1877, 2215, 2272, 2289, 2980, 3069, 3426
TatI WGTACW 4 cut(s) 44, 128, 1096, 3619
TauI GCSGC 5 cut(s) 138, 320, 2726, 3235, 3238
TfiI GAWTC 8 cut(s) 272, 1035, 1138, 1997, 2225, 2411, 2742, 2823
TscAI CASTG 6 cut(s) 631, 904, 1176, 1421, 2087, 3493
TseFI GTSAC 3 cut(s) 1807, 2510, 3374
Tsp45I GTSAC 3 cut(s) 1807, 2510, 3374
TspGWI ACGGA 1 cut(s) 3600
TspRI CASTG 6 cut(s) 631, 904, 1176, 1421, 2087, 3493
VpaK11BI GGWCC 4 cut(s) 1049, 1373, 1841, 1868
XagI CCTNNNNNAGG 1 cut(s) 2817
XapI RAATTY 6 cut(s) 213, 443, 1161, 2384, 2666, 2807
XbaI TCTAGA 1 cut(s) 2380
XceI RCATGY 4 cut(s) 135, 802, 3217, 3366
XcmI CCANNNNNNNNNTGG 1 cut(s) 359
XmnI GAANNNNTTC 1 cut(s) 1918
XspI CTAG 8 cut(s) 1196, 1727, 1937, 2052, 2220, 2381, 2565, 3599
ZrmI AGTACT 1 cut(s) 3621
Zsp2I ATGCAT 2 cut(s) 811, 1959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.