MD12G1183600.v1.1

Regulatory-associated protein of TOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002483
Physical Location & Seq
Reverse (-)
26367345 .. 26378184
10840 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1183600.v1.1.491

Sequence Viewer

Length: 4047 bp
ATGGCATTGGGGGATTTGATGGCCTCCCGGTTTTCGCAATCCTCGGTGGTGGTGGTCTCCAGCCAGTTGGACGACTGCGCGTCCAGCCACGATGACGGAGATTTGAGCTCGCAGAGAAGGGAATCAGAAACTGCCAGTAGTAGCTATGGAAATGCCACCGCTACCACCGCCACAAGCTTGGCCTACTTGCCCCAAACCACTGTATTGTGTGAACTTCGGCACGATGCTTTCGAGGCTTGCGTGCCCACCGGACCATCGGATAGTGGTTTAGTATCGAAATGGCGGCCTAAAGACCGAATGAAGACGGGATGTGTAGCTCTGGTATTATGTTTAAACATTAGCGTTGATCCGCCCGATGTAATTAAGATATCTCCCTGTGCCAGAATGGAGTGCTGGATAGATCCATTTGCAATGGCTCCACAAAAAGCTCTTGAAACAATTGGTAAAACCTTGAGTCAACAGTACGAAAGGTGGCAACCAAGGGCTCGCTACAAGGTTCAGCTTGATCCTACGGTAGAAGAAGTGAAGAAACTTTGTAATACATGCCGCAAATATGCCAAGTCCGAGAGAGTTCTTTTCCATTATAATGGACATGGTGTACCCAAGCCAACTGCTAATGGTGAAATTTGGCTCTTTAATAAGAGTTATACACAGTATATCCCCTTGCCAATTAGTGACCTCGATTCTTGGTTGAAGACACCTTCAATATATGTGTTTGATTGTTCTGCTGCTGGGATGATAGTCAATTCTTTCATTGAGCTTCATGATTGGGGTAGCTCTAGCTCATCTGGATCTGCAAGGGATTGTATCCTGCTAGCGGCTTGTGAAGCACACGAGACTCTTCCACAGAGTGCTGAATTTCCTGCTGATGTGTTTACTTCTTGCCTCACAACTCCCATCAAGATGGCATTAAGATGGTTCTGCACACGATCATTACTTCATGAATCTCTTGATTATTTGCTAATAGATAAAATCCCTGGCCGCCAAAATGACCGGAAAACACTTTTGGGCGAGTTGAATTGGATTTTTACTGCAGTCACTGATACGATTGCATGGAATGTTCTCCCTCACGATCTTTTCCAGAGACTGTTCAGACAAGACCTGCTAGTTGCCAGCCTTTTTCGCAATTTTTTACTTGCTGAGCGAATTATGCGATCCGCAAATTGCTCTCCGATCTCTCACCCACAGTTGCCTCCAACCCATCAGCATCATATGTGGGATGCATGGGACATGGCTGCTGAGATATGCCTTTCTCAGCTTCCACTATTGGTTGAGGATCCTAATGCGGAGTTCCAGCCAAGCCCATTTTTCACTGAGCAGTTGACTGCTTTTGAGGTATGGCTTGACCATGGGTCTGAAAACAAGAAACCACCAGAGCAATTGCCTATTGTTCTTCAGGTGTTACTTAGTCAATGCCACCGATTTCGAGCTCTGGTTCTTCTGGGAAGATTCCTTGATATGGGACCCTGGGCTGTAGATCTGGCCTTGTCTGTTGGGATATTTCCGTATGTTCTGAAGCTGTTACAAACAATGACACCTGAACTACGACATATTCTTGTATTTATATGGACAAAAATTCTTGCTCTTGATAAGTCATGTCAGGTTGATCTAGTGAAGGATGGGGGTCATACATATTTCATTAGGTTTCTTGATAGTATGGAAGCATATCCAGAACAACGTGCAATGGCTGCTTTTGTTTTAGCTGTAATTGTCGATGGGCATAGACGTGGGCAGGAGGCTTGTCTTGAAGCAGATTTGATACATGTTTGCTTGAAGCACCTTCAGGGTCCAACTCTAAATGATACACAGACAGAACCACTATTTCTTCAGTGGCTTTGCCTCTGTCTGGGAAAGCTGTGGGAGGATTTTACAGAGGCCCAAATATTTGGTTTGCAGGCAGATGCCTATTCAATATGTGCTCCTCTACTTTCTGAGCCCCAACCAGAGGTTAGAGCTTCTGCTGTTTTCGCACTGGGTACCCTTCTTGATGTGGGTTCAGGCTCATGTAGAGATGGCGTTGGTGGAGAAGAAGAAAACGATGATGATGAAAAGATTAGAGCTGAAATTAGTATTATTAGAAGTCTATTAAGTGTTGCTTCGGATGGCAGCCCTCTGGTCAGGGCCGAAGTTGCTGTTGCACTGGGACGCTTTGCCTTTGGCCACAACAAGCACCTCAAGTCAATAGCTGCTGCATATTGGAAACCACAATCTAATTCTCTTTTGAATTCCTTCCCCTCTTTGGCTCATATTAAAGGTTGTGTTGTTTCATCTCAAATTGGTCCACTGTTGAGAGGAACCAATGATAACTCATTGGGTCGAGATGGGAGAGTCTCCACCAGCAGCCCTCTAGCTAGTTCTGGAATTATGCATGGATCACCATTGTCTGACGATTCATCTCATCATTCTGATTCTGGAATATTAAATGATGGTGTGAGCAATGGTGTTGTCAACCACTCAACACCTAAACCCCTGGACAATGCAATGTATTCGCAGTGTGTATTGGCTATGTGTACCTTAGCCAAGGATCCATCTCCACGCATTGCAAGCCTTGGTAGGCGGGTTTTGGCCATTATAGGGATTGAACAAGTGGTGGCCAAACCCGTAAAGTCCACTGGTAACAGTGTTCGACCTGGTGAATCCATTACAGCAACTCCGACTCCCAGTTTTGGTGGATTAGCTCGGTCTTCTTCATGGTTAGATATGAATGGAGGTCACATGCCATTCCGAACTCCTCCTGTCAGCCCTCCTCAGCCAAATCACTTAACTGGAATACGGAGAGTATGCTCTTTAGACTTCAGGCCTCATCTAATGAGTCCAGATTCTGGATTAGCGGATCCACTTTTAGGTTCTGGGGGAACTTCTGGAGCCTCGGAACGCAGTTTTCTTCCTCAATCAGCCATCTACAATTGGGGTTGTGGGCACTTTTCCAAGCCACTTCTCATTGTCGCGGATGACAGTAAAGAAGTACTAAGTAGAAGAGAGGAGAGAGAAAAATTTGCCTTGGAGCACATTGCTAAATGCCAGCACTCTTCTGTGAGCAAGCTTAATAATCAAATTGCTAGCTGGGACACCAAGTTTGAGACGGGTACAAAAACAATCTTGCTGAAACCATTTTCTCCTATTGTGGTCGGTGCAGACGAGAATGAGCAAATCAGGGTGTGGAATTACCAAGAGCAAAAGGAGGCTACCCTTCTCAACAGCTTCAATAATCATGATTTTCCTGACAAAGGAATCTCCAAGCTCTGCCTTGTCAATGAGCTTGATGACAGCTTGCTTCTTGCTGCTTCAAGTGATGGAAACATTCGAATTTGGAAAGATTACACTTTGAAGGGTCAACAGAAGCTTGTCACTGCATTTTCTTCAATTCAAGGTCATAAACCTGGGGTGCGAAGTTTGAATGCTGTTGTGGATTGGCAACAACAATCTGGATATCTGTATGCTTCTGGCGAACTATCATCCATTATGCTTTGGGATCTGGATAAAGAGCAGCTTATTAATTCTATTCCTTCTTCATCAGATTGTAGCATCTCAGCCCTGTCTGCTTCTCAAGTTCACGGGGGTCACTTTGCAGCTGGCTTTGTAGATGGTTCTGTCAGACTTTATGATGTGCGAACTCCTGAAATGCTTATCTGTTCAACCCAGCCACACACTCAGAAGGTGGAAAGAGTTGTGGGGATCGGCTTTCAACCCGGGCTTGATCCTTCCAAGATTGTAAGTGCATCTCAAGCTGGTGACATTCAGTTCCTTGATATCAGAAACGGCAGGGATGCCTACCTCACGATTGAAGCTCACCGGGGTTCACTGACTGCTTTAGCTGTTCATAGGCATGCCCCAATCATTGCCAGTGGCTCGGCCAAACAGCTCATAAAAGTCTTCAGTTTGGAGGGTGAACAATTAGGCACAATAAGATACTACCCTTCCTTTATGGCCCAAAAGATTGGTCCTGTTAGTTCCCTAGCCTTCCATCCCTACGAAGTGCTGCTTGCAGCTGGTGCTGCAGATGCTTGCGCATCCATTTATGCCGACGACAACTCTCAAGCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0001558 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008361 GO:0009267 GO:0009605 GO:0009719 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009893 GO:0009894 GO:0009987 GO:0009991 GO:0010033 GO:0010073 GO:0010074 GO:0010154 GO:0010243 GO:0010492 GO:0010506 GO:0010562 GO:0010604 GO:0016043 GO:0019220 GO:0019222 GO:0019827 GO:0022414 GO:0023052 GO:0030307 GO:0030674 GO:0031323 GO:0031325 GO:0031329 GO:0031399 GO:0031401 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032535 GO:0032991 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040008 GO:0042221 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043200 GO:0043549 GO:0044093 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045927 GO:0045937 GO:0048316 GO:0048507 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060090 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071840 GO:0071900 GO:0071902 GO:0080008 GO:0080090 GO:0090066 GO:0098727 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04714 ko04910 ko05206 map04136 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04714 map04910 map05206
Pfam Domains
Protein Families
HEAT Raptor_N WD40

Protein Analysis

1349

Amino Acids

147.86

Weight (kDa)

5.92

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raptor_N PF14538 100 - 251 1.5e-71 Raptor N-terminal CASPase like domain
WD40_WDHD1_1st PF24817 1032 - 1179 2.2e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 1041 - 1212 2.7e-12 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 1042 - 1210 4.2e-06 TEP-1 second beta-propeller
WD40_Prp19 PF24814 1043 - 1221 4.9e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 1088 - 1341 1.3e-18 THOC3 beta-propeller domain
WDR55 PF24796 1090 - 1294 6.3e-10 WDR55
WD40_CDC20-Fz PF24807 1134 - 1220 5.9e-06 CDC20/Fizzy WD40 domain
WD40_CDC20-Fz PF24807 1135 - 1339 1.5e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 1146 - 1340 8.3e-16 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 1153 - 1292 9.6e-10 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 1233 - 1340 8.4e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
Acc16I TGCGCA 1 cut(s) 4009
Acc36I ACCTGC 1 cut(s) 1110
Acc65I GGTACC 1 cut(s) 1976
AccB1I GGYRCC 1 cut(s) 1976
AccB7I CCANNNNNTGG 1 cut(s) 2822
AccII CGCG 2 cut(s) 80, 2948
AcoI YGGCCR 5 cut(s) 979, 2158, 2565, 2592, 3852
AcsI RAATTY 6 cut(s) 624, 857, 1575, 2224, 2993, 3306
AcuI CTGAAG 6 cut(s) 1379, 1535, 1766, 1811, 2779, 3859
AdeI CACNNNGTG 1 cut(s) 851
AfaI GTAC 6 cut(s) 464, 600, 1978, 2512, 2967, 3088
AflIII ACRYGT 1 cut(s) 1762
AhdI GACNNNNNGTC 2 cut(s) 79, 1351
AjiI CACGTC 1 cut(s) 1727
AjnI CCWGG 5 cut(s) 976, 1466, 2469, 2629, 3379
AjuI GAANNNNNNNTTGG 2 cut(s) 989, 1021
Alw21I GWGCWC 4 cut(s) 110, 1432, 1921, 3009
Alw26I GTCTC 5 cut(s) 61, 830, 1078, 2335, 3074
AlwNI CAGNNNCTG 4 cut(s) 131, 1040, 1087, 2822
Ama87I CYCGRG 1 cut(s) 3689
ApoI RAATTY 6 cut(s) 624, 857, 1575, 2224, 2993, 3306
ArsI GACNNNNNNTTYG 4 cut(s) 3059, 3091, 3925, 3957
AseI ATTAAT 1 cut(s) 3495
Asp700I GAANNNNTTC 1 cut(s) 2228
Asp718I GGTACC 1 cut(s) 1976
AspLEI GCGC 2 cut(s) 80, 4010
AspS9I GGNCC 8 cut(s) 251, 1463, 1787, 1876, 2121, 2279, 3928, 3941
AsuC2I CCSGG 4 cut(s) 28, 3690, 3691, 3794
AsuHPI GGTGA 7 cut(s) 632, 1172, 2367, 2645, 3743, 3782, 3899
AsuII TTCGAA 1 cut(s) 3304
AsuNHI GCTAGC 2 cut(s) 814, 3059
AvaI CYCGRG 1 cut(s) 3689
AvaII GGWCC 5 cut(s) 251, 1463, 1787, 2279, 3941
BaeGI GKGCMC 2 cut(s) 246, 2922
BalI TGGCCA 3 cut(s) 2160, 2567, 2594
BamHI GGATCC 3 cut(s) 1276, 2524, 2833
BanI GGYRCC 1 cut(s) 1976
BanII GRGCYC 4 cut(s) 110, 487, 1432, 1938
BauI CACGAG 1 cut(s) 833
BbsI GAAGAC 4 cut(s) 308, 701, 2676, 3865
Bbv12I GWGCWC 4 cut(s) 110, 1432, 1921, 3009
BbvCI CCTCAGC 1 cut(s) 2748
BceAI ACGGC 1 cut(s) 3775
BcgI CGANNNNNNTGC 2 cut(s) 2469, 2503
BciT130I CCWGG 5 cut(s) 978, 1468, 2471, 2631, 3381
BciVI GTATCC 1 cut(s) 818
BcnI CCSGG 4 cut(s) 28, 3690, 3691, 3794
BcoDI GTCTC 5 cut(s) 61, 830, 1078, 2335, 3074
BfaI CTAG 8 cut(s) 780, 815, 1106, 1610, 2348, 2352, 3060, 3956
BfmI CTRYAG 3 cut(s) 1032, 1473, 3996
BfuAI ACCTGC 1 cut(s) 1110
BfuI GTATCC 1 cut(s) 818
BglII AGATCT 1 cut(s) 1477
BlpI GCTNAGC 1 cut(s) 1140
BmcAI AGTACT 1 cut(s) 2967
Bme1390I CCNGG 9 cut(s) 28, 978, 1468, 2471, 2631, 3381, 3690, 3691, 3794
Bme18I GGWCC 5 cut(s) 251, 1463, 1787, 2279, 3941
BmeRI GACNNNNNGTC 2 cut(s) 79, 1351
BmeT110I CYCGRG 1 cut(s) 3689
BmgBI CACGTC 1 cut(s) 1727
BmgT120I GGNCC 8 cut(s) 251, 1463, 1787, 1876, 2121, 2279, 3928, 3941
BmrFI CCNGG 9 cut(s) 28, 978, 1468, 2471, 2631, 3381, 3690, 3691, 3794
BmrI ACTGGG 3 cut(s) 1982, 2150, 2655
BmsI GCATC 9 cut(s) 214, 1210, 1216, 1891, 3534, 3728, 3757, 3991, 4019
BmtI GCTAGC 2 cut(s) 818, 3063
BmuI ACTGGG 3 cut(s) 1982, 2150, 2655
BpiI GAAGAC 4 cut(s) 308, 701, 2676, 3865
BpmI CTGGAG 2 cut(s) 43, 2883
Bpu10I CCTNAGC 2 cut(s) 2515, 2748
Bpu1102I GCTNAGC 1 cut(s) 1140
Bpu14I TTCGAA 1 cut(s) 3304
BpuEI CTTGAG 5 cut(s) 472, 2159, 3531, 3708, 4020
BpuMI CCSGG 4 cut(s) 28, 3690, 3691, 3794
BsaI GGTCTC 1 cut(s) 61
BsaWI WCCGGW 2 cut(s) 248, 993
BsaXI ACNNNNNCTCC 4 cut(s) 2635, 2641, 2665, 2671
Bse1I ACTGG 8 cut(s) 64, 135, 1977, 2145, 2617, 2661, 2770, 3843
Bse3DI GCAATG 7 cut(s) 417, 1689, 2443, 2487, 2538, 3009, 3837
BseBI CCWGG 5 cut(s) 978, 1468, 2471, 2631, 3381
BseMI GCAATG 7 cut(s) 417, 1689, 2443, 2487, 2538, 3009, 3837
BseMII CTCAG 8 cut(s) 1131, 1230, 1268, 1305, 1923, 2762, 3543, 3665
BseNI ACTGG 8 cut(s) 64, 135, 1977, 2145, 2617, 2661, 2770, 3843
BseRI GAGGAG 4 cut(s) 1911, 2721, 2736, 2996
BseSI GKGCMC 2 cut(s) 246, 2922
BseYI CCCAGC 3 cut(s) 731, 3063, 3639
BsgI GTGCAG 2 cut(s) 907, 3153
Bsh1236I CGCG 2 cut(s) 80, 2948
BshNI GGYRCC 1 cut(s) 1976
BsiHKAI GWGCWC 4 cut(s) 110, 1432, 1921, 3009
BsiHKCI CYCGRG 1 cut(s) 3689
BsiSI CCGG 5 cut(s) 28, 249, 994, 3690, 3793
BslFI GGGAC 4 cut(s) 1241, 1476, 2157, 3080
BsmAI GTCTC 5 cut(s) 61, 830, 1078, 2335, 3074
BsmBI CGTCTC 1 cut(s) 3074
BsmFI GGGAC 4 cut(s) 1241, 1476, 2157, 3080
BsmI GAATGC 1 cut(s) 3403
Bso31I GGTCTC 1 cut(s) 61
BsoBI CYCGRG 1 cut(s) 3689
Bsp119I TTCGAA 1 cut(s) 3304
Bsp1286I GDGCHC 8 cut(s) 110, 246, 487, 1432, 1921, 1938, 2922, 3009
Bsp1720I GCTNAGC 1 cut(s) 1140
Bsp19I CCATGG 1 cut(s) 1348
BspCNI CTCAG 8 cut(s) 1132, 1231, 1267, 1306, 1924, 2761, 3542, 3664
BspFNI CGCG 2 cut(s) 80, 2948
BspHI TCATGA 3 cut(s) 763, 940, 3211
BspMAI CTGCAG 2 cut(s) 1036, 4000
BspMI ACCTGC 1 cut(s) 1110
BspOI GCTAGC 2 cut(s) 818, 3063
BspT104I TTCGAA 1 cut(s) 3304
BspT107I GGYRCC 1 cut(s) 1976
BspTNI GGTCTC 1 cut(s) 61
BsrDI GCAATG 7 cut(s) 417, 1689, 2443, 2487, 2538, 3009, 3837
BsrI ACTGG 8 cut(s) 64, 135, 1977, 2145, 2617, 2661, 2770, 3843
BssSI CACGAG 1 cut(s) 833
BssT1I CCWWGG 5 cut(s) 479, 1348, 2520, 2548, 3000
Bst2BI CACGAG 1 cut(s) 833
Bst2UI CCWGG 5 cut(s) 978, 1468, 2471, 2631, 3381
Bst4CI ACNGT 9 cut(s) 202, 462, 514, 654, 1089, 1188, 2286, 2621, 2957
Bst6I CTCTTC 3 cut(s) 846, 2971, 3034
BstAPI GCANNNNNTGC 2 cut(s) 1688, 3992
BstBI TTCGAA 1 cut(s) 3304
BstDSI CCRYGG 1 cut(s) 1348
BstENI CCTNNNNNAGG 1 cut(s) 3225
BstFNI CGCG 2 cut(s) 80, 2948
BstHHI GCGC 2 cut(s) 80, 4010
BstMAI GTCTC 5 cut(s) 61, 830, 1078, 2335, 3074
BstNI CCWGG 5 cut(s) 978, 1468, 2471, 2631, 3381
BstNSI RCATGY 4 cut(s) 546, 1766, 2719, 3830
BstSCI CCNGG 9 cut(s) 26, 976, 1466, 2469, 2629, 3379, 3688, 3689, 3792
BstSFI CTRYAG 3 cut(s) 1032, 1473, 3996
BstSLI GKGCMC 2 cut(s) 246, 2922
BstUI CGCG 2 cut(s) 80, 2948
BstV2I GAAGAC 4 cut(s) 308, 701, 2676, 3865
BstX2I RGATCY 7 cut(s) 400, 791, 1276, 1477, 2524, 2833, 3472
BstXI CCANNNNNNTGG 6 cut(s) 67, 178, 587, 904, 1886, 3938
BstYI RGATCY 7 cut(s) 400, 791, 1276, 1477, 2524, 2833, 3472
BsuI GTATCC 1 cut(s) 818
BtgI CCRYGG 1 cut(s) 1348
BtrI CACGTC 1 cut(s) 1727
BtsI GCAGTG 2 cut(s) 2498, 3348
BveI ACCTGC 1 cut(s) 1110
CaiI CAGNNNCTG 4 cut(s) 131, 1040, 1087, 2822
CciI TCATGA 3 cut(s) 763, 940, 3211
CfoI GCGC 2 cut(s) 80, 4010
Cfr13I GGNCC 8 cut(s) 251, 1463, 1787, 1876, 2121, 2279, 3928, 3941
Cfr9I CCCGGG 1 cut(s) 3689
CseI GACGC 2 cut(s) 69, 2154
CsiI ACCWGGT 1 cut(s) 2629
Csp6I GTAC 6 cut(s) 463, 599, 1977, 2511, 2966, 3087
CviQI GTAC 6 cut(s) 463, 599, 1977, 2511, 2966, 3087
DraI TTTAAA 1 cut(s) 333
DraIII CACNNNGTG 1 cut(s) 851
DriI GACNNNNNGTC 2 cut(s) 79, 1351
EaeI YGGCCR 5 cut(s) 979, 2158, 2565, 2592, 3852
Eam1104I CTCTTC 3 cut(s) 846, 2971, 3034
Eam1105I GACNNNNNGTC 2 cut(s) 79, 1351
EarI CTCTTC 3 cut(s) 846, 2971, 3034
EciI GGCGGA 1 cut(s) 339
Ecl136II GAGCTC 2 cut(s) 108, 1430
Eco130I CCWWGG 5 cut(s) 479, 1348, 2520, 2548, 3000
Eco147I AGGCCT 1 cut(s) 2800
Eco24I GRGCYC 4 cut(s) 110, 487, 1432, 1938
Eco31I GGTCTC 1 cut(s) 61
Eco32I GATATC 3 cut(s) 369, 3431, 3751
Eco47I GGWCC 5 cut(s) 251, 1463, 1787, 2279, 3941
Eco53kI GAGCTC 2 cut(s) 108, 1430
Eco57I CTGAAG 6 cut(s) 1379, 1535, 1766, 1811, 2779, 3859
Eco88I CYCGRG 1 cut(s) 3689
EcoICRI GAGCTC 2 cut(s) 108, 1430
EcoNI CCTNNNNNAGG 1 cut(s) 3225
EcoO109I RGGNCCY 1 cut(s) 1463
EcoRI GAATTC 1 cut(s) 2224
EcoRII CCWGG 5 cut(s) 976, 1466, 2469, 2629, 3379
EcoRV GATATC 3 cut(s) 369, 3431, 3751
EcoT14I CCWWGG 5 cut(s) 479, 1348, 2520, 2548, 3000
EcoT22I ATGCAT 2 cut(s) 1225, 2370
EcoT38I GRGCYC 4 cut(s) 110, 487, 1432, 1938
ErhI CCWWGG 5 cut(s) 479, 1348, 2520, 2548, 3000
Esp3I CGTCTC 1 cut(s) 3074
FalI AAGNNNNNCTT 6 cut(s) 2080, 2112, 3474, 3506, 3966, 3998
FaqI GGGAC 4 cut(s) 1241, 1476, 2157, 3080
FauI CCCGC 1 cut(s) 2550
FauNDI CATATG 1 cut(s) 1212
FriOI GRGCYC 4 cut(s) 110, 487, 1432, 1938
FspBI CTAG 8 cut(s) 780, 815, 1106, 1610, 2348, 2352, 3060, 3956
FspI TGCGCA 1 cut(s) 4009
GlaI GCGC 2 cut(s) 79, 4009
GsaI CCCAGC 3 cut(s) 735, 3067, 3643
GsuI CTGGAG 2 cut(s) 43, 2883
HapII CCGG 5 cut(s) 28, 249, 994, 3690, 3793
HgaI GACGC 2 cut(s) 69, 2154
HhaI GCGC 2 cut(s) 80, 4010
Hin6I GCGC 2 cut(s) 78, 4008
HinP1I GCGC 2 cut(s) 78, 4008
HincII GTYRAC 4 cut(s) 458, 1323, 2449, 3335
HindII GTYRAC 4 cut(s) 458, 1323, 2449, 3335
HindIII AAGCTT 3 cut(s) 175, 3041, 3341
HpaII CCGG 5 cut(s) 28, 249, 994, 3690, 3793
HphI GGTGA 7 cut(s) 632, 1172, 2367, 2645, 3743, 3782, 3899
Hpy99I CGWCG 1 cut(s) 4028
HpyCH4III ACNGT 9 cut(s) 202, 462, 514, 654, 1089, 1188, 2286, 2621, 2957
HpyCH4IV ACGT 2 cut(s) 1678, 1726
HpySE526I ACGT 2 cut(s) 1678, 1726
HspAI GCGC 2 cut(s) 78, 4008
KflI GGGWCCC 1 cut(s) 1463
KpnI GGTACC 1 cut(s) 1980
LmnI GCTCC 4 cut(s) 421, 1924, 2864, 3004
LweI GCATC 9 cut(s) 214, 1210, 1216, 1891, 3534, 3728, 3757, 3991, 4019
MabI ACCWGGT 1 cut(s) 2629
MaeI CTAG 8 cut(s) 780, 815, 1106, 1610, 2348, 2352, 3060, 3956
MaeII ACGT 2 cut(s) 1678, 1726
MaeIII GTNAC 9 cut(s) 674, 1036, 1401, 1521, 2615, 2711, 3346, 3560, 3731
MfeI CAATTG 3 cut(s) 438, 1379, 2905
MflI RGATCY 7 cut(s) 400, 791, 1276, 1477, 2524, 2833, 3472
MhlI GDGCHC 8 cut(s) 110, 246, 487, 1432, 1921, 1938, 2922, 3009
MlsI TGGCCA 3 cut(s) 2160, 2567, 2594
MluNI TGGCCA 3 cut(s) 2160, 2567, 2594
MlyI GAGTC 5 cut(s) 463, 832, 2337, 2650, 2821
MmeI TCCRAC 4 cut(s) 48, 1220, 1814, 2678
Mox20I TGGCCA 3 cut(s) 2160, 2567, 2594
Mph1103I ATGCAT 2 cut(s) 1225, 2370
MroXI GAANNNNTTC 1 cut(s) 2228
MscI TGGCCA 3 cut(s) 2160, 2567, 2594
MslI CAYNNNNRTG 5 cut(s) 585, 902, 913, 1725, 3825
Msp20I TGGCCA 3 cut(s) 2160, 2567, 2594
MspA1I CMGCKG 2 cut(s) 3572, 3989
MspI CCGG 5 cut(s) 28, 249, 994, 3690, 3793
MspR9I CCNGG 9 cut(s) 28, 978, 1468, 2471, 2631, 3381, 3690, 3691, 3794
MssI GTTTAAAC 1 cut(s) 333
MunI CAATTG 3 cut(s) 438, 1379, 2905
Mva1269I GAATGC 1 cut(s) 3403
MvaI CCWGG 5 cut(s) 978, 1468, 2471, 2631, 3381
MvnI CGCG 2 cut(s) 80, 2948
NciI CCSGG 4 cut(s) 28, 3690, 3691, 3794
NcoI CCATGG 1 cut(s) 1348
NdeI CATATG 1 cut(s) 1212
NheI GCTAGC 2 cut(s) 814, 3059
NmeAIII GCCGAG 1 cut(s) 3830
NmuCI GTSAC 6 cut(s) 674, 1036, 2711, 3346, 3560, 3731
NsbI TGCGCA 1 cut(s) 4009
NsiI ATGCAT 2 cut(s) 1225, 2370
NspI RCATGY 4 cut(s) 546, 1766, 2719, 3830
NspV TTCGAA 1 cut(s) 3304
PaeI GCATGC 1 cut(s) 3830
PagI TCATGA 3 cut(s) 763, 940, 3211
PasI CCCWGGG 1 cut(s) 1467
PceI AGGCCT 1 cut(s) 2800
PciI ACATGT 1 cut(s) 1762
PcsI WCGNNNNNNNCGW 3 cut(s) 228, 237, 3135
PctI GAATGC 1 cut(s) 3403
PdmI GAANNNNTTC 1 cut(s) 2228
PfeI GAWTC 9 cut(s) 122, 683, 944, 1449, 2390, 2408, 2636, 2819, 3231
PflMI CCANNNNNTGG 1 cut(s) 2822
PleI GAGTC 5 cut(s) 462, 832, 2336, 2650, 2820
PmeI GTTTAAAC 1 cut(s) 333
PpsI GAGTC 5 cut(s) 462, 832, 2336, 2650, 2820
PpuMI RGGWCCY 1 cut(s) 1463
PscI ACATGT 1 cut(s) 1762
PshBI ATTAAT 1 cut(s) 3495
PsiI TTATAA 1 cut(s) 585
Psp124BI GAGCTC 2 cut(s) 110, 1432
Psp5II RGGWCCY 1 cut(s) 1463
Psp6I CCWGG 5 cut(s) 976, 1466, 2469, 2629, 3379
PspFI CCCAGC 3 cut(s) 731, 3063, 3639
PspGI CCWGG 5 cut(s) 976, 1466, 2469, 2629, 3379
PspPI GGNCC 8 cut(s) 251, 1463, 1787, 1876, 2121, 2279, 3928, 3941
PspPPI RGGWCCY 1 cut(s) 1463
PstI CTGCAG 2 cut(s) 1036, 4000
PstNI CAGNNNCTG 4 cut(s) 131, 1040, 1087, 2822
PsuI RGATCY 7 cut(s) 400, 791, 1276, 1477, 2524, 2833, 3472
PvuII CAGCTG 2 cut(s) 3572, 3989
RsaI GTAC 6 cut(s) 464, 600, 1978, 2512, 2967, 3088
RsaNI GTAC 6 cut(s) 463, 599, 1977, 2511, 2966, 3087
RseI CAYNNNNRTG 5 cut(s) 585, 902, 913, 1725, 3825
SacI GAGCTC 2 cut(s) 110, 1432
Sau96I GGNCC 8 cut(s) 251, 1463, 1787, 1876, 2121, 2279, 3928, 3941
ScaI AGTACT 1 cut(s) 2967
SchI GAGTC 5 cut(s) 463, 832, 2337, 2650, 2821
ScrFI CCNGG 9 cut(s) 28, 978, 1468, 2471, 2631, 3381, 3690, 3691, 3794
SduI GDGCHC 8 cut(s) 110, 246, 487, 1432, 1921, 1938, 2922, 3009
SexAI ACCWGGT 1 cut(s) 2629
SfaNI GCATC 9 cut(s) 214, 1210, 1216, 1891, 3534, 3728, 3757, 3991, 4019
SfcI CTRYAG 3 cut(s) 1032, 1473, 3996
SfuI TTCGAA 1 cut(s) 3304
SinI GGWCC 5 cut(s) 251, 1463, 1787, 2279, 3941
SmaI CCCGGG 1 cut(s) 3691
SmiMI CAYNNNNRTG 5 cut(s) 585, 902, 913, 1725, 3825
SmlI CTYRAG 5 cut(s) 451, 2174, 3546, 3723, 4035
SmoI CTYRAG 5 cut(s) 451, 2174, 3546, 3723, 4035
SphI GCATGC 1 cut(s) 3830
SseBI AGGCCT 1 cut(s) 2800
SspI AATATT 2 cut(s) 1884, 2418
SspMI CTAG 8 cut(s) 780, 815, 1106, 1610, 2348, 2352, 3060, 3956
SstI GAGCTC 2 cut(s) 110, 1432
StuI AGGCCT 1 cut(s) 2800
StyD4I CCNGG 9 cut(s) 26, 976, 1466, 2469, 2629, 3379, 3688, 3689, 3792
StyI CCWWGG 5 cut(s) 479, 1348, 2520, 2548, 3000
TaaI ACNGT 9 cut(s) 202, 462, 514, 654, 1089, 1188, 2286, 2621, 2957
TaiI ACGT 2 cut(s) 1681, 1729
TaqI TCGA 8 cut(s) 231, 275, 681, 1426, 1713, 2317, 2626, 3304
TaqII GACCGA 2 cut(s) 309, 2670
TatI WGTACW 1 cut(s) 2965
TauI GCSGC 4 cut(s) 286, 549, 821, 984
TfiI GAWTC 9 cut(s) 122, 683, 944, 1449, 2390, 2408, 2636, 2819, 3231
TseFI GTSAC 6 cut(s) 674, 1036, 2711, 3346, 3560, 3731
Tsp45I GTSAC 6 cut(s) 674, 1036, 2711, 3346, 3560, 3731
TspGWI ACGGA 3 cut(s) 111, 1494, 2788
TspMI CCCGGG 1 cut(s) 3689
Van91I CCANNNNNTGG 1 cut(s) 2822
VpaK11BI GGWCC 5 cut(s) 251, 1463, 1787, 2279, 3941
VspI ATTAAT 1 cut(s) 3495
XagI CCTNNNNNAGG 1 cut(s) 3225
XapI RAATTY 6 cut(s) 624, 857, 1575, 2224, 2993, 3306
XceI RCATGY 4 cut(s) 546, 1766, 2719, 3830
XmaI CCCGGG 1 cut(s) 3689
XmnI GAANNNNTTC 1 cut(s) 2228
XspI CTAG 8 cut(s) 780, 815, 1106, 1610, 2348, 2352, 3060, 3956
ZrmI AGTACT 1 cut(s) 2967
Zsp2I ATGCAT 2 cut(s) 1225, 2370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.