Rroxscaffold_6G00418430

Regulatory-associated protein of TOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002483
Physical Location & Seq
Forward (+)
39949389 .. 39960170
10782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00418430.1

Sequence Viewer

Length: 3636 bp
ATGGCATTGGGGGATTTGATGGCCTCCAGGTTTTCGCAATCCTCGGTGGTCGTCGTCTCCAATCACTTCGACGACTGCGCTTCCAGCCACGACGACGGTGATGTGAGCTCCCAGAGAAGGGAATCAGAGACTGCGAGTAGTAGCTATGAGAATGCCACGGGCACAACCACTACAAGCATGGCGTACTTGCCCCAAACCATTGTCTTGTGTGAGCTTCGCCACGACGCGTTCGAGGCTCGAGTCCCTACAGGTCCTTCTGATAGCGGATTGGTTTCGAAATGGCGGCCTAAAGACCGAATGAAGACGGGATGTGTAGCTCTAGTATTATGTTTAAACATCAGCGTTGATCCGCCCGATGTAATTAAGATATCTCCCTGTGCCAGAATGGAGTGCTGGATAGATCCATTTTCTATGGCTCCAACAAAAGCTCTTGAAACAATTGGTAAAACCTTGAGTACACAGTATGAAAGATGGCAACCAAGGGCTAAATACAGGGTTCAGCTTGATCCTACTATAGATGAAGTCAAGAAGCTTTGTAGTACATGCCGCAAATATGCCAAGTCTGAGAGAGTTCTTTTCCATTATAACGGTCATGGTGTACCCAAGCCAACTGCTAATGGTGAAATTTGGCTATTTAATAAGAGTTATACACAGTATATTCCCTTGCCAATTAGCGACCTTGATTCCTGGTTGAAGACACCTTCAATATATGTCTTTGACTGTTCTGCCGCTGGCATGATTGTTAATTCGTTCACTGAGCTCCATGATTGGGCTGGCTCTAACTCTGGATCTCCAAGAGATTGTATCCTGCTTGCTGCTTGTGAACCACACGAGACTCTTCCACAGAGTGCGGAATTTCCTGCTGATGTCTTTACTTCTTGCCTCACAACTCCCATCAAGATGGCTTTAAGATGGGATGCGTGGGACATGGCTGCTGAGATATGCCTTTCTCAGCTTCCATTGTTGGTTGAGGATCCTAATGCTGAGTTTCAGCCAAGTCCATTTTTCACTGAGCAGTTGACTGCTTTTGAGGTATGGCTTGACCACGGGTCTGAACACAAGAAACCACCAGAGCAGTTGCCTATTGTTCTTCAGGTCTTACTTAGTCAATGCCATCGGTTTCGAGCTCTGGTTCTTCTCGGGAGATTCCTTGATATGGGACCCTGGGCTGTAGATCTGGCATTGTCTGTTGGAATATTTCCATATGTACTGAAGCTGTTGCAAACGATGACACCTGAACTACGACAGATTCTTGTATTCATTTGGACAAAAATTCTTGCACTGGATAAGTCATGCCAGGTGGACCTAGTGAAGGATAAGGGTCATACATATTTCATCAGGTTTCTTGATAGCATGGAAGCATATCCAGAGCAGCGTGCAATGGCTGCTTTTGTTTTGGCTGTAATAGTTGATGGCCATAGACGTGGCCAGGAAGCTTGTATTGAAGCAGGTTTAATCCATGTTTGTTTGAAGCACCTTCAGGGTCCAACACCAAATGACACACAAACTGAACCCCTATTTCTTCAGTGGCTTTGCCTCTGTCTGGGGAAACTATGGGAGGATTTCACAGATGCCCAAATATTTGGTTTGCAGGCAGATGCCCCTGCAATATGTGCTCCTCTGCTTGCTGAGCCCCAACCAGAGGTGAGGGCTTCTGCTGTTTTCGCATTGGGCACCCTGCTTGATGTGGGTTCAGGATCATGTAGAGATGGCAATGGTGGAGACGATGAGTATGATGATGAGGAAAAGATAAGGGCTGAAATTAGTATCGTTAAAAGCCTATTAAGTGTTGTTTCAGATGGAAGCCCTCTTGTCAGAGCAGAAGTTGCTGTTGCTCTAGGACGCTTTGCCTTTGGACACAACAAGCACCTGAAGTCGATTGCTGCTGCCTATTGGAAACCACAGTCTAATTCTCTTGTGACTTCCTTGTCTGCTTTGGTTAGTATTAAAGGTCCTGGTAGTGCTTCATCTCATATTGGTCCACTTTCGAGAGTTGGCAATGACAGCTCATTGGTTCGGGATGGAAGAGTTTCTACCAGCAGTCCCCTGGCCAGTTCTGGAATTATGCATGGATCTCCATTGTCTGATGATTTATCTCATCATTCTGATTCTGGAATATTAGATGATGGTGGGAGCAATGGTATTGTTAACCATTTGACCCCCAAACCCCTGGACAGTCAAATGTATTCTCAGTGTGTATTGGCTATGTGTAACTTAGCCAAAGATCCATCTCCACGAATTGCAAAAATTGGTCGCCGAGTATTAGCCATTATAGGGATTGAGCAAGTGGTGGCAAAACCTGTAAAGTCCACGGGTAACAGTGTTCGACCTAGTGAATCCATTACAGCGGGTCCAAGTCAGAGTCTTGCTGCTGGATTGGCTCGATCGTCTTCATGGTTCGATATGAATGGAGGTCATTTGCCTTTCCGAACTCCACCTGTCAGCCCTCCTAGGGCAAATTACTTGGCTGGAATGCGAAGAGTTTGCTCTCTAGAATTTAGGCCTCATCTAATGAGTCCAGATTCAGGCTTAGCTGATCCCCTTTTAGGTTCTGGGGGAACTTTTGGTGCCTCAGAACGAAGTTTTCTTCCTCAATCAACAATCTACAATTGGAGTTGCGGTCACTTTTCCAAGCCACTTCTTATAGTGGCAGATGACACTGTTAGCAAGCTTAATAATCAAATTGCAAGCTGGGACACAAAATTTGAAACGGGTACGAAAAGCATTTTGCTGGAACCATTCTCTCCTATTGTGATTGCTGCTGATGAGAATGAACGAATCAGGGTATGGAATTACCAAGAGGCTAAGGAGGCTATCCTTCTGAACAGCTTTGATAACCATGATTTTCCCGACAAAGGAATCTCCAAGCTTTGCTTTGTGAATGAACTTGATGACAGCTTGCTTCTTGCTGCCTCAAGTGATGGAAACATTCGGATTTGGAAAGATTATACTTTGAAGGGTAAACAGAAACTTGTTACTGCATTTTCTTCAATTCAAGGACATAAACCTGGTGTTCGAAGTCTTAATGCTGTTGTGGATTGGCAGCAGCAATCTGGATATCTGTATGCCTCTGGTGAGCTATCATCCATTATGCTCTGGGATCTCGATAAGGAGCAGCTTGTTAATTCTATTCATTCTCAATCAGACTGTAGCATTTCAGCATTGTCTGCATCTCAAGTTCATGGAAGTCAACTCACAGCTGGCTTTGTTGACGGTTCTGTTAGGCTCTATGATGTGCGAACTCCTGACATGCTTGTCTGTGAAATGCGGCCGCACACTCCAAAGGTTGTAGGAAACGCACAGAAGATAGAAAGAGTTGTTGGGAACACTCAGAAGGTAGAAAGAGTTGTGGGGATTGGCTTTCAACCGGGGCTTGATCCTTCTAAGATTGTTAGTGCATGTCAGGCTGGTGACATTCAGTTCCTTGATATCAGAAATAGTAAAGATCCCTACCTCACAATCGAAGCTCACAGGGGTTCACTCACAGCTTTAGCTGTTCATAGACACGCTCCGCTCATTGCCAGTGGCTCAGCCAAGCAGCTCATAAAAGTCTTTAGTTTGGAGGGCGAACAATTAGGCACCATTAGATACTACCCTTCCTTTATGGCCCAAAAATTGGTCCTGTTAGCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0001558 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008361 GO:0009267 GO:0009605 GO:0009719 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009893 GO:0009894 GO:0009987 GO:0009991 GO:0010033 GO:0010073 GO:0010074 GO:0010154 GO:0010243 GO:0010492 GO:0010506 GO:0010562 GO:0010604 GO:0016043 GO:0019220 GO:0019222 GO:0019827 GO:0022414 GO:0023052 GO:0030307 GO:0030674 GO:0031323 GO:0031325 GO:0031329 GO:0031399 GO:0031401 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032535 GO:0032991 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040008 GO:0042221 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043200 GO:0043549 GO:0044093 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045927 GO:0045937 GO:0048316 GO:0048507 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060090 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071840 GO:0071900 GO:0071902 GO:0080008 GO:0080090 GO:0090066 GO:0098727 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04714 ko04910 ko05206 map04136 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04714 map04910 map05206
Pfam Domains
Protein Families
HEAT Raptor_N WD40

Protein Analysis

1211

Amino Acids

132.35

Weight (kDa)

5.91

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raptor_N PF14538 100 - 251 1.2e-70 Raptor N-terminal CASPase like domain
WD40_WDHD1_1st PF24817 902 - 1056 9.8e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 916 - 1085 2.7e-12 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 918 - 1108 3.4e-07 TEP-1 second beta-propeller
WD40_Prp19 PF24814 922 - 1093 8.9e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 966 - 1188 7.9e-14 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 1010 - 1098 6.2e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 1022 - 1187 1.1e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AasI GACNNNNNNGTC 2 cut(s) 1927, 3254
Acc36I ACCTGC 1 cut(s) 1439
AccB1I GGYRCC 3 cut(s) 1673, 2567, 3578
AccB7I CCANNNNNTGG 1 cut(s) 3616
AccBSI CCGCTC 1 cut(s) 3514
AccII CGCG 1 cut(s) 227
AcoI YGGCCR 4 cut(s) 1414, 1426, 2049, 3269
AcsI RAATTY 5 cut(s) 624, 854, 1272, 2495, 2702
AcuI CTGAAG 5 cut(s) 1076, 1232, 1463, 1508, 1892
AdeI CACNNNGTG 1 cut(s) 848
AfaI GTAC 6 cut(s) 185, 457, 541, 600, 1209, 2716
AflIII ACRYGT 1 cut(s) 225
AhdI GACNNNNNGTC 1 cut(s) 1048
AjiI CACGTC 1 cut(s) 1424
AjnI CCWGG 9 cut(s) 26, 686, 1163, 1296, 1428, 1954, 2046, 2169, 3007
AjuI GAANNNNNNNTTGG 2 cut(s) 3303, 3335
Alw21I GWGCWC 4 cut(s) 110, 762, 1129, 1618
Alw26I GTCTC 4 cut(s) 61, 122, 827, 1716
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 2 cut(s) 237, 1139
AoxI GGCC 8 cut(s) 21, 284, 1414, 1426, 2049, 2501, 3269, 3606
ApoI RAATTY 5 cut(s) 624, 854, 1272, 2495, 2702
ArsI GACNNNNNNTTYG 2 cut(s) 2687, 2719
Asp700I GAANNNNTTC 1 cut(s) 2029
AspA2I CCTAGG 1 cut(s) 2450
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 9 cut(s) 251, 1160, 1303, 1484, 1952, 1979, 2351, 3607, 3619
AsuC2I CCSGG 1 cut(s) 3369
AsuHPI GGTGA 5 cut(s) 110, 632, 1657, 3086, 3422
AsuII TTCGAA 2 cut(s) 275, 3016
AvaI CYCGRG 2 cut(s) 237, 1139
AvaII GGWCC 8 cut(s) 251, 1160, 1303, 1484, 1952, 1979, 2351, 3619
AvrII CCTAGG 1 cut(s) 2450
BaeGI GKGCMC 2 cut(s) 164, 1676
BalI TGGCCA 3 cut(s) 1416, 1428, 2051
BamHI GGATCC 1 cut(s) 973
BanI GGYRCC 3 cut(s) 1673, 2567, 3578
BanII GRGCYC 4 cut(s) 110, 762, 1129, 1635
BarI GAAGNNNNNNTAC 2 cut(s) 2017, 2049
BauI CACGAG 1 cut(s) 830
BbsI GAAGAC 3 cut(s) 308, 701, 2382
Bbv12I GWGCWC 4 cut(s) 110, 762, 1129, 1618
BcgI CGANNNNNNTGC 2 cut(s) 2466, 2500
BciT130I CCWGG 9 cut(s) 28, 688, 1165, 1298, 1430, 1956, 2048, 2171, 3009
BciVI GTATCC 1 cut(s) 815
BcnI CCSGG 1 cut(s) 3369
BcoDI GTCTC 4 cut(s) 61, 122, 827, 1716
BfaI CTAG 7 cut(s) 320, 1307, 1838, 2331, 2451, 2492, 3634
BfmI CTRYAG 4 cut(s) 246, 513, 1170, 3148
BfuAI ACCTGC 1 cut(s) 1439
BfuI GTATCC 1 cut(s) 815
BglII AGATCT 1 cut(s) 1174
BlnI CCTAGG 1 cut(s) 2450
BlpI GCTNAGC 3 cut(s) 1629, 2530, 3529
Bme18I GGWCC 8 cut(s) 251, 1160, 1303, 1484, 1952, 1979, 2351, 3619
BmeRI GACNNNNNGTC 1 cut(s) 1048
BmeT110I CYCGRG 2 cut(s) 237, 1139
BmgBI CACGTC 1 cut(s) 1424
BmgT120I GGNCC 9 cut(s) 251, 1160, 1303, 1484, 1952, 1979, 2351, 3607, 3619
BmsI GCATC 4 cut(s) 907, 1561, 1588, 3179
BpiI GAAGAC 3 cut(s) 308, 701, 2382
BpmI CTGGAG 1 cut(s) 10
Bpu10I CCTNAGC 1 cut(s) 2805
Bpu1102I GCTNAGC 3 cut(s) 1629, 2530, 3529
Bpu14I TTCGAA 2 cut(s) 275, 3016
BpuEI CTTGAG 3 cut(s) 472, 2899, 3159
BpuMI CCSGG 1 cut(s) 3369
BsaXI ACNNNNNCTCC 4 cut(s) 2125, 2155, 2605, 2635
Bse1I ACTGG 3 cut(s) 1287, 2052, 3522
Bse3DI GCAATG 5 cut(s) 1386, 1720, 2005, 2143, 3516
BseBI CCWGG 9 cut(s) 28, 688, 1165, 1298, 1430, 1956, 2048, 2171, 3009
BseGI GGATG 4 cut(s) 314, 922, 2026, 3083
BseMI GCAATG 5 cut(s) 1386, 1720, 2005, 2143, 3516
BseNI ACTGG 3 cut(s) 1287, 2052, 3522
BseRI GAGGAG 1 cut(s) 1608
BseSI GKGCMC 2 cut(s) 164, 1676
BseX3I CGGCCG 1 cut(s) 3269
BseYI CCCAGC 1 cut(s) 2691
Bsh1236I CGCG 1 cut(s) 227
Bsh1285I CGRYCG 2 cut(s) 2387, 3272
BshFI GGCC 8 cut(s) 23, 286, 1416, 1428, 2051, 2503, 3271, 3608
BshNI GGYRCC 3 cut(s) 1673, 2567, 3578
BsiEI CGRYCG 2 cut(s) 2387, 3272
BsiHKAI GWGCWC 4 cut(s) 110, 762, 1129, 1618
BsiHKCI CYCGRG 2 cut(s) 237, 1139
BsiSI CCGG 1 cut(s) 3368
BslFI GGGAC 5 cut(s) 227, 938, 1173, 2028, 2708
BsmAI GTCTC 4 cut(s) 61, 122, 827, 1716
BsmBI CGTCTC 2 cut(s) 61, 1716
BsmFI GGGAC 5 cut(s) 227, 938, 1173, 2028, 2708
BsmI GAATGC 2 cut(s) 157, 2478
BsnI GGCC 8 cut(s) 23, 286, 1416, 1428, 2051, 2503, 3271, 3608
BsoBI CYCGRG 2 cut(s) 237, 1139
Bsp119I TTCGAA 2 cut(s) 275, 3016
Bsp1286I GDGCHC 7 cut(s) 110, 164, 762, 1129, 1618, 1635, 1676
Bsp1720I GCTNAGC 3 cut(s) 1629, 2530, 3529
BspANI GGCC 8 cut(s) 23, 286, 1416, 1428, 2051, 2503, 3271, 3608
BspFNI CGCG 1 cut(s) 227
BspMI ACCTGC 1 cut(s) 1439
BspT104I TTCGAA 2 cut(s) 275, 3016
BspT107I GGYRCC 3 cut(s) 1673, 2567, 3578
BsrBI CCGCTC 1 cut(s) 3514
BsrDI GCAATG 5 cut(s) 1386, 1720, 2005, 2143, 3516
BsrI ACTGG 3 cut(s) 1287, 2052, 3522
BssSI CACGAG 1 cut(s) 830
BssT1I CCWWGG 2 cut(s) 479, 2450
Bst2BI CACGAG 1 cut(s) 830
Bst2UI CCWGG 9 cut(s) 28, 688, 1165, 1298, 1430, 1956, 2048, 2171, 3009
Bst6I CTCTTC 3 cut(s) 843, 2020, 2473
BstAPI GCANNNNNTGC 4 cut(s) 1385, 1613, 1826, 3167
BstBI TTCGAA 2 cut(s) 275, 3016
BstDSI CCRYGG 3 cut(s) 156, 1045, 2310
BstENI CCTNNNNNAGG 1 cut(s) 1310
BstF5I GGATG 4 cut(s) 314, 922, 2026, 3083
BstFNI CGCG 1 cut(s) 227
BstHHI GCGC 1 cut(s) 80
BstMAI GTCTC 4 cut(s) 61, 122, 827, 1716
BstMCI CGRYCG 2 cut(s) 2387, 3272
BstNI CCWGG 9 cut(s) 28, 688, 1165, 1298, 1430, 1956, 2048, 2171, 3009
BstNSI RCATGY 3 cut(s) 546, 3253, 3402
BstSFI CTRYAG 4 cut(s) 246, 513, 1170, 3148
BstSLI GKGCMC 2 cut(s) 164, 1676
BstUI CGCG 1 cut(s) 227
BstV2I GAAGAC 3 cut(s) 308, 701, 2382
BstX2I RGATCY 8 cut(s) 400, 788, 973, 1174, 2072, 2224, 3100, 3445
BstXI CCANNNNNNTGG 4 cut(s) 901, 1424, 1583, 2170
BstYI RGATCY 8 cut(s) 400, 788, 973, 1174, 2072, 2224, 3100, 3445
BstZI CGGCCG 1 cut(s) 3269
BsuI GTATCC 1 cut(s) 815
BsuRI GGCC 8 cut(s) 23, 286, 1416, 1428, 2051, 2503, 3271, 3608
BtgI CCRYGG 3 cut(s) 156, 1045, 2310
BtrI CACGTC 1 cut(s) 1424
BtsCI GGATG 4 cut(s) 314, 922, 2026, 3083
BtsIMutI CAGTG 8 cut(s) 753, 1008, 1280, 1532, 2198, 2326, 2658, 3529
BveI ACCTGC 1 cut(s) 1439
CaiI CAGNNNCTG 1 cut(s) 131
CciNI GCGGCCGC 1 cut(s) 3269
CfoI GCGC 1 cut(s) 80
Cfr13I GGNCC 9 cut(s) 251, 1160, 1303, 1484, 1952, 1979, 2351, 3607, 3619
CseI GACGC 2 cut(s) 233, 1851
CsiI ACCWGGT 1 cut(s) 3007
Csp6I GTAC 6 cut(s) 184, 456, 540, 599, 1208, 2715
CviQI GTAC 6 cut(s) 184, 456, 540, 599, 1208, 2715
DraI TTTAAA 1 cut(s) 333
DraIII CACNNNGTG 1 cut(s) 848
DrdI GACNNNNNNGTC 2 cut(s) 1927, 3254
DriI GACNNNNNGTC 1 cut(s) 1048
DseDI GACNNNNNNGTC 2 cut(s) 1927, 3254
EaeI YGGCCR 4 cut(s) 1414, 1426, 2049, 3269
EagI CGGCCG 1 cut(s) 3269
Eam1104I CTCTTC 3 cut(s) 843, 2020, 2473
Eam1105I GACNNNNNGTC 1 cut(s) 1048
EarI CTCTTC 3 cut(s) 843, 2020, 2473
EciI GGCGGA 1 cut(s) 339
Ecl136II GAGCTC 3 cut(s) 108, 760, 1127
EclXI CGGCCG 1 cut(s) 3269
Eco130I CCWWGG 2 cut(s) 479, 2450
Eco147I AGGCCT 1 cut(s) 2503
Eco24I GRGCYC 4 cut(s) 110, 762, 1129, 1635
Eco32I GATATC 3 cut(s) 369, 3059, 3430
Eco47I GGWCC 8 cut(s) 251, 1160, 1303, 1484, 1952, 1979, 2351, 3619
Eco52I CGGCCG 1 cut(s) 3269
Eco53kI GAGCTC 3 cut(s) 108, 760, 1127
Eco57I CTGAAG 5 cut(s) 1076, 1232, 1463, 1508, 1892
Eco88I CYCGRG 2 cut(s) 237, 1139
EcoICRI GAGCTC 3 cut(s) 108, 760, 1127
EcoNI CCTNNNNNAGG 1 cut(s) 1310
EcoO109I RGGNCCY 3 cut(s) 251, 1160, 1952
EcoRII CCWGG 9 cut(s) 26, 686, 1163, 1296, 1428, 1954, 2046, 2169, 3007
EcoRV GATATC 3 cut(s) 369, 3059, 3430
EcoT14I CCWWGG 2 cut(s) 479, 2450
EcoT22I ATGCAT 1 cut(s) 2070
EcoT38I GRGCYC 4 cut(s) 110, 762, 1129, 1635
ErhI CCWWGG 2 cut(s) 479, 2450
Esp3I CGTCTC 2 cut(s) 61, 1716
FalI AAGNNNNNCTT 2 cut(s) 2858, 2890
FaqI GGGAC 5 cut(s) 227, 938, 1173, 2028, 2708
FauI CCCGC 1 cut(s) 2341
FauNDI CATATG 1 cut(s) 1204
FokI GGATG 4 cut(s) 321, 929, 2033, 3070
FriOI GRGCYC 4 cut(s) 110, 762, 1129, 1635
FspBI CTAG 7 cut(s) 320, 1307, 1838, 2331, 2451, 2492, 3634
GlaI GCGC 1 cut(s) 79
GsaI CCCAGC 1 cut(s) 2695
GsuI CTGGAG 1 cut(s) 10
HaeIII GGCC 8 cut(s) 23, 286, 1416, 1428, 2051, 2503, 3271, 3608
HapII CCGG 1 cut(s) 3368
HgaI GACGC 2 cut(s) 233, 1851
HhaI GCGC 1 cut(s) 80
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 4 cut(s) 1020, 2149, 3191, 3211
HindII GTYRAC 4 cut(s) 1020, 2149, 3191, 3211
HindIII AAGCTT 4 cut(s) 530, 1434, 2669, 2867
HpaI GTTAAC 1 cut(s) 2149
HpaII CCGG 1 cut(s) 3368
HphI GGTGA 5 cut(s) 110, 632, 1657, 3086, 3422
Hpy99I CGWCG 5 cut(s) 56, 74, 95, 98, 227
HpyCH4IV ACGT 1 cut(s) 1423
HpySE526I ACGT 1 cut(s) 1423
HspAI GCGC 1 cut(s) 78
KflI GGGWCCC 1 cut(s) 1160
KspAI GTTAAC 1 cut(s) 2149
LmnI GCTCC 7 cut(s) 113, 421, 765, 1621, 2133, 3112, 3514
LweI GCATC 4 cut(s) 907, 1561, 1588, 3179
MabI ACCWGGT 1 cut(s) 3007
MaeI CTAG 7 cut(s) 320, 1307, 1838, 2331, 2451, 2492, 3634
MaeII ACGT 1 cut(s) 1423
MaeIII GTNAC 6 cut(s) 1918, 2210, 2315, 2621, 2974, 3410
MbiI CCGCTC 1 cut(s) 3514
MfeI CAATTG 2 cut(s) 438, 2608
MflI RGATCY 8 cut(s) 400, 788, 973, 1174, 2072, 2224, 3100, 3445
MhlI GDGCHC 7 cut(s) 110, 164, 762, 1129, 1618, 1635, 1676
MlsI TGGCCA 3 cut(s) 1416, 1428, 2051
MluI ACGCGT 1 cut(s) 225
MluNI TGGCCA 3 cut(s) 1416, 1428, 2051
MlyI GAGTC 4 cut(s) 249, 829, 2371, 2524
MmeI TCCRAC 3 cut(s) 443, 1171, 1511
Mox20I TGGCCA 3 cut(s) 1416, 1428, 2051
Mph1103I ATGCAT 1 cut(s) 2070
MroXI GAANNNNTTC 1 cut(s) 2029
MscI TGGCCA 3 cut(s) 1416, 1428, 2051
MslI CAYNNNNRTG 2 cut(s) 899, 1422
Msp20I TGGCCA 3 cut(s) 1416, 1428, 2051
MspA1I CMGCKG 3 cut(s) 731, 2348, 3200
MspI CCGG 1 cut(s) 3368
MssI GTTTAAAC 1 cut(s) 333
MunI CAATTG 2 cut(s) 438, 2608
Mva1269I GAATGC 2 cut(s) 157, 2478
MvaI CCWGG 9 cut(s) 28, 688, 1165, 1298, 1430, 1956, 2048, 2171, 3009
MvnI CGCG 1 cut(s) 227
NciI CCSGG 1 cut(s) 3369
NdeI CATATG 1 cut(s) 1204
NmeAIII GCCGAG 1 cut(s) 2282
NmuCI GTSAC 3 cut(s) 1918, 2621, 3410
NotI GCGGCCGC 1 cut(s) 3269
NsiI ATGCAT 1 cut(s) 2070
NspI RCATGY 3 cut(s) 546, 3253, 3402
NspV TTCGAA 2 cut(s) 275, 3016
PaeR7I CTCGAG 1 cut(s) 237
PasI CCCWGGG 1 cut(s) 1164
PceI AGGCCT 1 cut(s) 2503
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PctI GAATGC 2 cut(s) 157, 2478
PdmI GAANNNNTTC 1 cut(s) 2029
PfeI GAWTC 9 cut(s) 122, 683, 1146, 1249, 2108, 2336, 2522, 2778, 2859
PflMI CCANNNNNTGG 1 cut(s) 3616
Ple19I CGATCG 1 cut(s) 2387
PleI GAGTC 4 cut(s) 248, 829, 2370, 2523
PmeI GTTTAAAC 1 cut(s) 333
PpsI GAGTC 4 cut(s) 248, 829, 2370, 2523
PpuMI RGGWCCY 3 cut(s) 251, 1160, 1952
PsiI TTATAA 1 cut(s) 585
Psp124BI GAGCTC 3 cut(s) 110, 762, 1129
Psp5II RGGWCCY 3 cut(s) 251, 1160, 1952
Psp6I CCWGG 9 cut(s) 26, 686, 1163, 1296, 1428, 1954, 2046, 2169, 3007
PspFI CCCAGC 1 cut(s) 2691
PspGI CCWGG 9 cut(s) 26, 686, 1163, 1296, 1428, 1954, 2046, 2169, 3007
PspPI GGNCC 9 cut(s) 251, 1160, 1303, 1484, 1952, 1979, 2351, 3607, 3619
PspPPI RGGWCCY 3 cut(s) 251, 1160, 1952
PspXI VCTCGAGB 1 cut(s) 237
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 8 cut(s) 400, 788, 973, 1174, 2072, 2224, 3100, 3445
PvuI CGATCG 1 cut(s) 2387
PvuII CAGCTG 1 cut(s) 3200
RsaI GTAC 6 cut(s) 185, 457, 541, 600, 1209, 2716
RsaNI GTAC 6 cut(s) 184, 456, 540, 599, 1208, 2715
RseI CAYNNNNRTG 2 cut(s) 899, 1422
SacI GAGCTC 3 cut(s) 110, 762, 1129
Sau96I GGNCC 9 cut(s) 251, 1160, 1303, 1484, 1952, 1979, 2351, 3607, 3619
SchI GAGTC 4 cut(s) 249, 829, 2371, 2524
SduI GDGCHC 7 cut(s) 110, 164, 762, 1129, 1618, 1635, 1676
SexAI ACCWGGT 1 cut(s) 3007
SfaNI GCATC 4 cut(s) 907, 1561, 1588, 3179
SfcI CTRYAG 4 cut(s) 246, 513, 1170, 3148
Sfr274I CTCGAG 1 cut(s) 237
SfuI TTCGAA 2 cut(s) 275, 3016
SinI GGWCC 8 cut(s) 251, 1160, 1303, 1484, 1952, 1979, 2351, 3619
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 2 cut(s) 899, 1422
SmlI CTYRAG 4 cut(s) 237, 451, 2914, 3174
SmoI CTYRAG 4 cut(s) 237, 451, 2914, 3174
SseBI AGGCCT 1 cut(s) 2503
SspI AATATT 3 cut(s) 1197, 1581, 2118
SspMI CTAG 7 cut(s) 320, 1307, 1838, 2331, 2451, 2492, 3634
SstI GAGCTC 3 cut(s) 110, 762, 1129
StuI AGGCCT 1 cut(s) 2503
StyI CCWWGG 2 cut(s) 479, 2450
TaiI ACGT 1 cut(s) 1426
TaqII GACCGA 1 cut(s) 309
TatI WGTACW 3 cut(s) 455, 539, 1207
TauI GCSGC 5 cut(s) 286, 549, 731, 3271, 3274
TfiI GAWTC 9 cut(s) 122, 683, 1146, 1249, 2108, 2336, 2522, 2778, 2859
TscAI CASTG 8 cut(s) 760, 1015, 1287, 1532, 2198, 2326, 2665, 3529
TseFI GTSAC 3 cut(s) 1918, 2621, 3410
Tsp45I GTSAC 3 cut(s) 1918, 2621, 3410
TspRI CASTG 8 cut(s) 760, 1015, 1287, 1532, 2198, 2326, 2665, 3529
Van91I CCANNNNNTGG 1 cut(s) 3616
VpaK11BI GGWCC 8 cut(s) 251, 1160, 1303, 1484, 1952, 1979, 2351, 3619
XagI CCTNNNNNAGG 1 cut(s) 1310
XapI RAATTY 5 cut(s) 624, 854, 1272, 2495, 2702
XbaI TCTAGA 1 cut(s) 2491
XceI RCATGY 3 cut(s) 546, 3253, 3402
XcmI CCANNNNNNNNNTGG 3 cut(s) 175, 770, 2044
XhoI CTCGAG 1 cut(s) 237
XmaJI CCTAGG 1 cut(s) 2450
XmnI GAANNNNTTC 1 cut(s) 2029
XspI CTAG 7 cut(s) 320, 1307, 1838, 2331, 2451, 2492, 3634
Zsp2I ATGCAT 1 cut(s) 2070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.