MD04G1169600.v1.1

Regulatory-associated protein of TOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002483
Physical Location & Seq
Reverse (-)
26023088 .. 26033921
10834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1169600.v1.1.491

Sequence Viewer

Length: 4038 bp
ATGGCATTGGGGGATTTGATGGCCTCCCGGTTTTCGCAATCCTCGGTGGTGGTCTACAGTCAGTTGGACGACTGCGCCTCCAGCCACGACGACGGCGATTTGAGCTCCCAGAGAAGGGAATCAGAGACTGCCAGTAGTGGTTATGGAAATGCCACCGCCACGAGCATGGCCTACTTGCCCCAAACCGTTGTATTGTGTGAACTTCGACACTATGCTTTCGAGGCTTGCGTGCCCACCGGACCATCGGATAGTGGTTTGGTCTCGAAATGGCGGCCTAAAGACCGAATGAAGACGGGATGTGTAGCTCTAGTATTATGTTTAAACATTAGCGTTGATCCGCCTGATGTAATTAAGATATCTCCCTGTGCCAGAATGGAGTGCTGGATAGATCCCTTTTCTATGGCTCCACAAAAAGCTCTAGAAACAATTGGTAAAACCTTGAGTCAACAGTACGAAAGGTGGCAACCCAGGGCTCGCTACAAGGTTCAGCTTGATCCTACAGTAGAAGAAGTGAAGAAACTTTGTAATACATGCCGCAAATACGCCAAGTCCGAGAGAGTTCTTTTCCACTATAATGGACATGGTGTACCCAAGCCAACTGCTAATGGTGAAATTTGGCTCTTTAATAAGGTTTTTACCAATTTCAAGCCTGCTAGTTATACACAGTATATTCCCTTGCCGATTAGTGACCTTGATTCGTGGTTGAAGACACCTTCAATATATGTGTTTGATTGTTCTGCTGCTGGGATGATAGTCAATTCTTTCATTGAGCTTTATGATTTGGGTGGCTCTAGCTCTTCTGGATCTGCAAGGGATTGTATCCTGCTGGCGGCTTGTGAACCACATGAAACTCTGCCACAAAGTGCTGAATTCCCTGCTGATGTGTTTACTTCTTGCCTGACAACTCCCATTAAGATGGCATTAAGATGGTTCTGCACACGATCATTACTTCATGAGTCTATTGATTATTCGCTAATCGATAAAATACCTGGCCGCCAAAATGACCGGAAAACCCTCCTTGGCGAGTTGAATTGGATTTTTACTGCGGTCACTGATACGATTGCATGGAATGTTCTCCCTCATGATCTTTTCCAGAGACTGTTCAGACAAGACCTGCTAGTTGCCAGCCTTTTTCGTAATTTTTTACTTGCTGAGCGAATTATGAGATCTGCAAATTGCTCTCCGATCTCTTACCCGCAGTTGCCTCCAACCCATCAGCATCATATGTGGGATGCATGGGACATGGCTGCTGAGATATGCCTTTCTCAGCTTCCATCATTGGTTGAGGATCCTAATGCAGAGTTCCAGCCAAGTCCATTTTTCACTGAGCAGTTGACTGCTTTTGAGGTATGGCTTGACCATGGGTCTGAACTCAAGAAACCACCAGAGCAGTTGCCTATTGTTCTTCAGGTGTTACTAAGTCAATGCCACCGATCTCGAGCTCTAGTTCTTCTTGGAAGATTCCTTGATATGGGATCCTGGGCTGTAGATCTGGCCTTGTCTGTTGGGATATTTCCATACGTTCTGAAGCTGTTACAAACAATGACACCTGAACTACGACAAATTCTTGTATTTATTTGGACAAAAATTCTTGCTCTTGATAAGTCATGTCAGGTTGATCTAGTGAAGGATGGGGGTCATACGTATTTCATCAGGTTTCTTGATAGTATGGAAGCGTATCCAGAACAGCGTGCAATGGCTGCTTTTGTTTTAGCTGTAATTGTCGATGGGCATAGACGCGGGCAGGAAGCTTGTATTGAAGCAAGTTTGATACATGTTTGCTTGAAGCACCTTCAGGGTCCAACTCTAAATGATACACAGACAGAACCACTATTTCTTCAGTGGCTTTGTCTCTGTCTTGGGAAGCTGTGGGAGGATTTCACAGAGGCCCAAATATTTGGTTTGCAGGCAGATGCCCCTGCAATATGTGCTTCTCTACTTTCTGAGCCCCAACCAGAGGTTAGGGCTTCTGCTGTTTTCGCACTGGGTACCCTGCTTGATGTGGGTTCAGGCAATGGTGTTGGTGGAGAAGAAGAATACGACGATGATAAAAAGATTAGAGCTGAAATTAGTATTGTTAGAAGTCTACTAAATGTTGCTTCAGATGGGAGCCCTCTGGTCAGGGCCGAAGTTGCTGTTGCACTGGGACGCTTTGCCTTTGGCCATAACAAGCACCTCAAGTCAATTGCCGCTGCATATTGGAAACCACAATCTAATTCTCTTTTGAATTCCTTGCCCTCTTTGGCTCATATGAAAGGTAGTGTTGTTTCATCTCAAATTGGTCCACTTTTGAGAGGGACCAATGACAACTATGGAAGAGTCTCCACCAGCAGCCCTCTAGCGAGTTCTGGAATTATGCATGGATCACCATTGTCTGACGATTCATCTCATCATTCTGATTCTGGAATATTAAATGATGGTGTGAGCAACGGTGTTGTCAACCACTCAACACCTGAACCCCTGGACAATGCAATTTACTCTCAGTGTGTATTGGCTATGTGTACTCTAGCCAAGGATCCATCTCCACGCATCGCAAGCCTTGGTAGGCGGGTTTTGGCCATTATAGGGATTGAACAAGTGGTGGCCAAACCCATGAAGTCCACTGGTAACAGTGTTCGACCTGGTGAATCCACTACAGCAACTCCGATTCCCAGTTTTTCTGGATTAGCTCGATCTTCTTCATGGTTTGATATGAATGGAGGTCATATGCCATTCCGAACTCCTCCTGTCAGCCCACCTCGGCCCAATTACTTAGCTGGAATGCGGAGAGTATACTCTTTAGAGCTCAGGCCTCATATAATGAGTCCAGATTCTGGATTAGCGGATCCACTTTTAGGTTCTGAGGGAACTTCTGGAGCCTCGGAACGCAGTTTTCTTCCTCAATCAACCATCTACAATTGGAGTTGTGGGCACTTTTCCAAGCCGCTTCTCATTGTGGCAGATGACAGTAAAGAAGTACTTAGTAGAAGAGAGGAGAGAGAAAAATTTGCCTTGGAGCACATTGCTAAATGCCAGCACTCGTCTGTGAGCAAGCTTAATAATCAAATTGCTAGCTGGGACACCAAATTTGAGACGGGTACAAAAACAATCTTGCTGAATCCATTTTCTCCTATTGTGGTTGCGGCAGACGAGAAAGAGCGAATCAGGGTGTGGAACTACCAAGAGCAGAAGGAGGCTACCCTTCTCAACAGCTTCGATAACCATGATTTTCCTGACAAAGGAATCGCCAAGCTCTGCCTTGTGAATGAGCTTGATGACAGCTTGCTTCTTGCTGCTTCAAGTGATGGACACATTCGAATTTGGAAAGATTACACTTTGAAGGGTAAGCAGAAGCTTGTCACTGCATTTTCTTCAATTCTAGGTCATAAACCTGGCGTGCGAAGTTTGAATGCTGTTGTGGATTGGCAACAACAATCTGGATATCTGTATGCTTCTGGCGAATTACCATCCATTATGCTTTGGGATCTGGATAAAGAGCAGCTTATTAATTCTATTCCTTCTTCATCAGATTGTAGCATCTCAGCACTGTCTGCTTCTCAAGTTCACGGTGGTCACTTTGCAGCTGGTTTCGTGGATGGTTCTGTCAGGCTTTATGATGTGCGAACTCCTGAAATGCTTGTCTGTTCAACCCGGCCACACACTCAGAAGGTGGAAAGAGTTGTGGGGATTGGCTTTCAACCTGGGCTGGATCCTGGCAAGATTGTAAGTGCATCTCAAGCTGGTGACATTCAGTTCCTTGATATCAGAAACGACAGGGATGCCTACCTCACGATTGAAGCTCACCGCGGTTCACTGACAGCTTTAGCTGTTCATAGGCATGCCCCAATCATTGCCAGTGGCTCGGCCAAACAGCTCATAAAAGTCTTCAGCTTGGAGGGCGAACAATTAGGCACAATAAGATACTACCCTTCCTTTATGGCCCAAAAGATTGGTCCTGTTAGTTGCCTAGCCTTCCATCCCTACGAAGTGCTGCTTGCAGCTGGTGCCGCAGATGCTTGCGCATCCATTTATGCCGACGACAACTCTCAAGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0001558 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008361 GO:0009267 GO:0009605 GO:0009719 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009893 GO:0009894 GO:0009987 GO:0009991 GO:0010033 GO:0010073 GO:0010074 GO:0010154 GO:0010243 GO:0010492 GO:0010506 GO:0010562 GO:0010604 GO:0016043 GO:0019220 GO:0019222 GO:0019827 GO:0022414 GO:0023052 GO:0030307 GO:0030674 GO:0031323 GO:0031325 GO:0031329 GO:0031399 GO:0031401 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032535 GO:0032991 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040008 GO:0042221 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043200 GO:0043549 GO:0044093 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045927 GO:0045937 GO:0048316 GO:0048507 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060090 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071840 GO:0071900 GO:0071902 GO:0080008 GO:0080090 GO:0090066 GO:0098727 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04714 ko04910 ko05206 map04136 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04714 map04910 map05206
Pfam Domains
Protein Families
HEAT Raptor_N WD40

Protein Analysis

1346

Amino Acids

147.95

Weight (kDa)

6.0

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raptor_N PF14538 96 - 255 3.6e-67 Raptor N-terminal CASPase like domain
WD40_WDHD1_1st PF24817 1018 - 1217 1.2e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 1038 - 1211 1.3e-10 WDR5 beta-propeller domain
WD40_Prp19 PF24814 1038 - 1218 1.5e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 1085 - 1338 4.5e-17 THOC3 beta-propeller domain
WDR55 PF24796 1087 - 1292 1.2e-08 WDR55
WD40_CDC20-Fz PF24807 1132 - 1336 4.6e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 1144 - 1337 1.1e-14 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 1150 - 1289 5.7e-09 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 1211 - 1337 2.6e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 4000
Acc36I ACCTGC 1 cut(s) 1122
Acc65I GGTACC 1 cut(s) 1988
AccB1I GGYRCC 2 cut(s) 1988, 3983
AccB7I CCANNNNNTGG 1 cut(s) 2813
AccI GTMKAC 3 cut(s) 54, 2086, 2772
AccII CGCG 2 cut(s) 1740, 3786
AcoI YGGCCR 6 cut(s) 991, 2161, 2556, 2583, 3632, 3843
AcsI RAATTY 8 cut(s) 612, 869, 1563, 1587, 2227, 2984, 3065, 3297
AcuI CTGAAG 6 cut(s) 1391, 1547, 1778, 1823, 2085, 3850
AdeI CACNNNGTG 1 cut(s) 863
AfaI GTAC 6 cut(s) 452, 588, 1990, 2503, 2958, 3079
AfiI CCNNNNNNNGG 9 cut(s) 114, 115, 829, 1471, 1957, 2565, 2813, 2834, 3218
AflIII ACRYGT 1 cut(s) 1774
AhdI GACNNNNNGTC 1 cut(s) 1363
AjnI CCWGG 8 cut(s) 467, 988, 1478, 2460, 2620, 3370, 3679, 3691
Alw21I GWGCWC 4 cut(s) 107, 1444, 2787, 3000
Alw26I GTCTC 6 cut(s) 119, 265, 1090, 1856, 2326, 3065
AlwNI CAGNNNCTG 3 cut(s) 128, 1099, 2813
Ama87I CYCGRG 1 cut(s) 1437
ApoI RAATTY 8 cut(s) 612, 869, 1563, 1587, 2227, 2984, 3065, 3297
ArsI GACNNNNNNTTYG 4 cut(s) 3050, 3082, 3916, 3948
AseI ATTAAT 1 cut(s) 3486
Asp718I GGTACC 1 cut(s) 1988
AspLEI GCGC 2 cut(s) 77, 4001
AspS9I GGNCC 9 cut(s) 239, 1799, 1888, 2124, 2282, 2298, 2742, 3919, 3932
AsuC2I CCSGG 2 cut(s) 28, 3631
AsuHPI GGTGA 5 cut(s) 620, 2358, 2636, 3734, 3773
AsuII TTCGAA 1 cut(s) 3295
AsuNHI GCTAGC 1 cut(s) 3050
AvaI CYCGRG 1 cut(s) 1437
AvaII GGWCC 5 cut(s) 239, 1799, 2282, 2298, 3932
BaeGI GKGCMC 2 cut(s) 234, 2913
BalI TGGCCA 3 cut(s) 2163, 2558, 2585
BamHI GGATCC 5 cut(s) 1288, 1475, 2515, 2824, 3688
BanI GGYRCC 2 cut(s) 1988, 3983
BanII GRGCYC 6 cut(s) 107, 475, 1444, 1950, 2114, 2787
BauI CACGAG 1 cut(s) 160
BbsI GAAGAC 3 cut(s) 296, 713, 3856
Bbv12I GWGCWC 4 cut(s) 107, 1444, 2787, 3000
BceAI ACGGC 1 cut(s) 109
BcgI CGANNNNNNTGC 2 cut(s) 3740, 3774
BciT130I CCWGG 8 cut(s) 469, 990, 1480, 2462, 2622, 3372, 3681, 3693
BciVI GTATCC 2 cut(s) 830, 1689
BcnI CCSGG 2 cut(s) 28, 3631
BcoDI GTCTC 6 cut(s) 119, 265, 1090, 1856, 2326, 3065
BfmI CTRYAG 4 cut(s) 55, 498, 1485, 2634
BfuAI ACCTGC 1 cut(s) 1122
BfuI GTATCC 2 cut(s) 830, 1689
BglII AGATCT 2 cut(s) 1166, 1489
BlpI GCTNAGC 1 cut(s) 1152
BmcAI AGTACT 1 cut(s) 2958
Bme18I GGWCC 5 cut(s) 239, 1799, 2282, 2298, 3932
BmeRI GACNNNNNGTC 1 cut(s) 1363
BmeT110I CYCGRG 1 cut(s) 1437
BmgT120I GGNCC 9 cut(s) 239, 1799, 1888, 2124, 2282, 2298, 2742, 3919, 3932
BmrI ACTGGG 3 cut(s) 1994, 2153, 2646
BmsI GCATC 9 cut(s) 1222, 1228, 1903, 2538, 3525, 3719, 3748, 3982, 4010
BmtI GCTAGC 1 cut(s) 3054
BmuI ACTGGG 3 cut(s) 1994, 2153, 2646
BpiI GAAGAC 3 cut(s) 296, 713, 3856
BpmI CTGGAG 2 cut(s) 64, 2874
Bpu10I CCTNAGC 1 cut(s) 2786
Bpu1102I GCTNAGC 1 cut(s) 1152
Bpu14I TTCGAA 1 cut(s) 3295
BpuEI CTTGAG 6 cut(s) 460, 1358, 2162, 3522, 3699, 4011
BpuMI CCSGG 2 cut(s) 28, 3631
Bsa29I ATCGAT 1 cut(s) 978
BsaAI YACGTR 1 cut(s) 1644
BsaI GGTCTC 1 cut(s) 265
BsaWI WCCGGW 2 cut(s) 236, 1005
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 2626, 2656
Bsc4I CCNNNNNNNGG 9 cut(s) 114, 115, 829, 1471, 1957, 2565, 2813, 2834, 3218
Bse1I ACTGG 6 cut(s) 132, 1989, 2148, 2608, 2652, 3834
Bse3DI GCAATG 4 cut(s) 1701, 2020, 3000, 3828
BseBI CCWGG 8 cut(s) 469, 990, 1480, 2462, 2622, 3372, 3681, 3693
BseCI ATCGAT 1 cut(s) 978
BseGI GGATG 9 cut(s) 302, 753, 1237, 1636, 3446, 3580, 3763, 3955, 4001
BseLI CCNNNNNNNGG 9 cut(s) 114, 115, 829, 1471, 1957, 2565, 2813, 2834, 3218
BseMI GCAATG 4 cut(s) 1701, 2020, 3000, 3828
BseNI ACTGG 6 cut(s) 132, 1989, 2148, 2608, 2652, 3834
BseRI GAGGAG 2 cut(s) 2712, 2987
BseSI GKGCMC 2 cut(s) 234, 2913
BseYI CCCAGC 2 cut(s) 743, 3054
BsgI GTGCAG 1 cut(s) 919
Bsh1236I CGCG 2 cut(s) 1740, 3786
BshNI GGYRCC 2 cut(s) 1988, 3983
BshVI ATCGAT 1 cut(s) 978
BsiHKAI GWGCWC 4 cut(s) 107, 1444, 2787, 3000
BsiHKCI CYCGRG 1 cut(s) 1437
BsiSI CCGG 4 cut(s) 28, 237, 1006, 3631
BslFI GGGAC 4 cut(s) 1253, 2160, 2311, 3071
BslI CCNNNNNNNGG 9 cut(s) 114, 115, 829, 1471, 1957, 2565, 2813, 2834, 3218
BsmAI GTCTC 6 cut(s) 119, 265, 1090, 1856, 2326, 3065
BsmBI CGTCTC 1 cut(s) 3065
BsmFI GGGAC 4 cut(s) 1253, 2160, 2311, 3071
BsmI GAATGC 2 cut(s) 2766, 3394
Bso31I GGTCTC 1 cut(s) 265
BsoBI CYCGRG 1 cut(s) 1437
Bsp119I TTCGAA 1 cut(s) 3295
Bsp1286I GDGCHC 9 cut(s) 107, 234, 475, 1444, 1950, 2114, 2787, 2913, 3000
Bsp1720I GCTNAGC 1 cut(s) 1152
Bsp19I CCATGG 1 cut(s) 1360
BspDI ATCGAT 1 cut(s) 978
BspFNI CGCG 2 cut(s) 1740, 3786
BspHI TCATGA 2 cut(s) 952, 1081
BspMI ACCTGC 1 cut(s) 1122
BspOI GCTAGC 1 cut(s) 3054
BspQI GCTCTTC 1 cut(s) 802
BspT104I TTCGAA 1 cut(s) 3295
BspT107I GGYRCC 2 cut(s) 1988, 3983
BspTNI GGTCTC 1 cut(s) 265
BsrDI GCAATG 4 cut(s) 1701, 2020, 3000, 3828
BsrI ACTGG 6 cut(s) 132, 1989, 2148, 2608, 2652, 3834
BssNAI GTATAC 1 cut(s) 2773
BssSI CACGAG 1 cut(s) 160
BssT1I CCWWGG 5 cut(s) 1018, 1360, 2511, 2539, 2991
Bst1107I GTATAC 1 cut(s) 2773
Bst2BI CACGAG 1 cut(s) 160
Bst2UI CCWGG 8 cut(s) 469, 990, 1480, 2462, 2622, 3372, 3681, 3693
Bst6I CTCTTC 3 cut(s) 802, 2311, 2962
BstAPI GCANNNNNTGC 4 cut(s) 1700, 1928, 3530, 3983
BstBAI YACGTR 1 cut(s) 1644
BstBI TTCGAA 1 cut(s) 3295
BstDSI CCRYGG 2 cut(s) 1360, 3784
BstENI CCTNNNNNAGG 1 cut(s) 3216
BstF5I GGATG 9 cut(s) 302, 753, 1237, 1636, 3446, 3580, 3763, 3955, 4001
BstFNI CGCG 2 cut(s) 1740, 3786
BstHHI GCGC 2 cut(s) 77, 4001
BstMAI GTCTC 6 cut(s) 119, 265, 1090, 1856, 2326, 3065
BstNI CCWGG 8 cut(s) 469, 990, 1480, 2462, 2622, 3372, 3681, 3693
BstNSI RCATGY 3 cut(s) 534, 1778, 3821
BstSFI CTRYAG 4 cut(s) 55, 498, 1485, 2634
BstSLI GKGCMC 2 cut(s) 234, 2913
BstSNI TACGTA 1 cut(s) 1644
BstUI CGCG 2 cut(s) 1740, 3786
BstV2I GAAGAC 3 cut(s) 296, 713, 3856
BstXI CCANNNNNNTGG 5 cut(s) 166, 575, 916, 1898, 3929
BstZ17I GTATAC 1 cut(s) 2773
Bsu15I ATCGAT 1 cut(s) 978
BsuI GTATCC 2 cut(s) 830, 1689
BsuTUI ATCGAT 1 cut(s) 978
BtgI CCRYGG 2 cut(s) 1360, 3784
BtgZI GCGATG 1 cut(s) 2515
BtsCI GGATG 9 cut(s) 302, 753, 1237, 1636, 3446, 3580, 3763, 3955, 4001
BtsI GCAGTG 1 cut(s) 3339
BveI ACCTGC 1 cut(s) 1122
CaiI CAGNNNCTG 3 cut(s) 128, 1099, 2813
CciI TCATGA 2 cut(s) 952, 1081
CfoI GCGC 2 cut(s) 77, 4001
Cfr13I GGNCC 9 cut(s) 239, 1799, 1888, 2124, 2282, 2298, 2742, 3919, 3932
Cfr42I CCGCGG 1 cut(s) 3787
ClaI ATCGAT 1 cut(s) 978
CseI GACGC 2 cut(s) 1746, 2157
CsiI ACCWGGT 1 cut(s) 2620
Csp6I GTAC 6 cut(s) 451, 587, 1989, 2502, 2957, 3078
CviQI GTAC 6 cut(s) 451, 587, 1989, 2502, 2957, 3078
DraI TTTAAA 1 cut(s) 321
DraIII CACNNNGTG 1 cut(s) 863
DriI GACNNNNNGTC 1 cut(s) 1363
EaeI YGGCCR 6 cut(s) 991, 2161, 2556, 2583, 3632, 3843
Eam1104I CTCTTC 3 cut(s) 802, 2311, 2962
Eam1105I GACNNNNNGTC 1 cut(s) 1363
EarI CTCTTC 3 cut(s) 802, 2311, 2962
EciI GGCGGA 1 cut(s) 327
Ecl136II GAGCTC 3 cut(s) 105, 1442, 2785
Eco105I TACGTA 1 cut(s) 1644
Eco130I CCWWGG 5 cut(s) 1018, 1360, 2511, 2539, 2991
Eco147I AGGCCT 1 cut(s) 2791
Eco24I GRGCYC 6 cut(s) 107, 475, 1444, 1950, 2114, 2787
Eco31I GGTCTC 1 cut(s) 265
Eco32I GATATC 3 cut(s) 357, 3422, 3742
Eco47I GGWCC 5 cut(s) 239, 1799, 2282, 2298, 3932
Eco53kI GAGCTC 3 cut(s) 105, 1442, 2785
Eco57I CTGAAG 6 cut(s) 1391, 1547, 1778, 1823, 2085, 3850
Eco88I CYCGRG 1 cut(s) 1437
EcoICRI GAGCTC 3 cut(s) 105, 1442, 2785
EcoNI CCTNNNNNAGG 1 cut(s) 3216
EcoRI GAATTC 2 cut(s) 869, 2227
EcoRII CCWGG 8 cut(s) 467, 988, 1478, 2460, 2620, 3370, 3679, 3691
EcoRV GATATC 3 cut(s) 357, 3422, 3742
EcoT14I CCWWGG 5 cut(s) 1018, 1360, 2511, 2539, 2991
EcoT22I ATGCAT 2 cut(s) 1237, 2361
EcoT38I GRGCYC 6 cut(s) 107, 475, 1444, 1950, 2114, 2787
ErhI CCWWGG 5 cut(s) 1018, 1360, 2511, 2539, 2991
Esp3I CGTCTC 1 cut(s) 3065
FalI AAGNNNNNCTT 6 cut(s) 2943, 2975, 3465, 3497, 3957, 3989
FaqI GGGAC 4 cut(s) 1253, 2160, 2311, 3071
FauI CCCGC 3 cut(s) 1203, 1733, 2541
FauNDI CATATG 3 cut(s) 1224, 2250, 2706
FblI GTMKAC 3 cut(s) 54, 2086, 2772
FokI GGATG 9 cut(s) 309, 760, 1244, 1643, 3433, 3587, 3770, 3942, 3988
FriOI GRGCYC 6 cut(s) 107, 475, 1444, 1950, 2114, 2787
FspI TGCGCA 1 cut(s) 4000
GlaI GCGC 2 cut(s) 76, 4000
GsaI CCCAGC 2 cut(s) 747, 3058
GsuI CTGGAG 2 cut(s) 64, 2874
HapII CCGG 4 cut(s) 28, 237, 1006, 3631
HgaI GACGC 2 cut(s) 1746, 2157
HhaI GCGC 2 cut(s) 77, 4001
Hin6I GCGC 2 cut(s) 75, 3999
HinP1I GCGC 2 cut(s) 75, 3999
HincII GTYRAC 3 cut(s) 446, 1335, 2440
HindII GTYRAC 3 cut(s) 446, 1335, 2440
HindIII AAGCTT 3 cut(s) 1749, 3032, 3332
HpaII CCGG 4 cut(s) 28, 237, 1006, 3631
HphI GGTGA 5 cut(s) 620, 2358, 2636, 3734, 3773
Hpy99I CGWCG 4 cut(s) 92, 95, 2045, 4019
HpyCH4IV ACGT 2 cut(s) 1521, 1643
HpySE526I ACGT 2 cut(s) 1521, 1643
HspAI GCGC 2 cut(s) 75, 3999
KpnI GGTACC 1 cut(s) 1992
KspI CCGCGG 1 cut(s) 3787
LguI GCTCTTC 1 cut(s) 802
LmnI GCTCC 5 cut(s) 110, 409, 2109, 2855, 2995
LweI GCATC 9 cut(s) 1222, 1228, 1903, 2538, 3525, 3719, 3748, 3982, 4010
MabI ACCWGGT 1 cut(s) 2620
MaeII ACGT 2 cut(s) 1521, 1643
MaeIII GTNAC 8 cut(s) 686, 1048, 1413, 1533, 2606, 3337, 3551, 3722
MfeI CAATTG 3 cut(s) 426, 2184, 2896
MhlI GDGCHC 9 cut(s) 107, 234, 475, 1444, 1950, 2114, 2787, 2913, 3000
MlsI TGGCCA 3 cut(s) 2163, 2558, 2585
MluNI TGGCCA 3 cut(s) 2163, 2558, 2585
MlyI GAGTC 4 cut(s) 451, 965, 2328, 2812
MmeI TCCRAC 3 cut(s) 45, 1232, 1826
Mox20I TGGCCA 3 cut(s) 2163, 2558, 2585
Mph1103I ATGCAT 2 cut(s) 1237, 2361
MscI TGGCCA 3 cut(s) 2163, 2558, 2585
MseI TTAA 8 cut(s) 320, 351, 624, 912, 923, 2411, 3036, 3486
MslI CAYNNNNRTG 5 cut(s) 164, 573, 914, 925, 3816
Msp20I TGGCCA 3 cut(s) 2163, 2558, 2585
MspA1I CMGCKG 4 cut(s) 2192, 3563, 3786, 3980
MspI CCGG 4 cut(s) 28, 237, 1006, 3631
MssI GTTTAAAC 1 cut(s) 321
MunI CAATTG 3 cut(s) 426, 2184, 2896
Mva1269I GAATGC 2 cut(s) 2766, 3394
MvaI CCWGG 8 cut(s) 469, 990, 1480, 2462, 2622, 3372, 3681, 3693
MvnI CGCG 2 cut(s) 1740, 3786
NciI CCSGG 2 cut(s) 28, 3631
NcoI CCATGG 1 cut(s) 1360
NdeI CATATG 3 cut(s) 1224, 2250, 2706
NheI GCTAGC 1 cut(s) 3050
NmeAIII GCCGAG 2 cut(s) 2719, 3821
NmuCI GTSAC 5 cut(s) 686, 1048, 3337, 3551, 3722
NsbI TGCGCA 1 cut(s) 4000
NsiI ATGCAT 2 cut(s) 1237, 2361
NspI RCATGY 3 cut(s) 534, 1778, 3821
NspV TTCGAA 1 cut(s) 3295
PaeI GCATGC 1 cut(s) 3821
PaeR7I CTCGAG 1 cut(s) 1437
PagI TCATGA 2 cut(s) 952, 1081
PasI CCCWGGG 1 cut(s) 468
PceI AGGCCT 1 cut(s) 2791
PciI ACATGT 1 cut(s) 1774
PciSI GCTCTTC 1 cut(s) 802
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 225, 549
PctI GAATGC 2 cut(s) 2766, 3394
PflMI CCANNNNNTGG 1 cut(s) 2813
PleI GAGTC 4 cut(s) 450, 964, 2327, 2811
PmeI GTTTAAAC 1 cut(s) 321
PpsI GAGTC 4 cut(s) 450, 964, 2327, 2811
Ppu21I YACGTR 1 cut(s) 1644
PscI ACATGT 1 cut(s) 1774
PshBI ATTAAT 1 cut(s) 3486
Psp124BI GAGCTC 3 cut(s) 107, 1444, 2787
Psp6I CCWGG 8 cut(s) 467, 988, 1478, 2460, 2620, 3370, 3679, 3691
PspFI CCCAGC 2 cut(s) 743, 3054
PspGI CCWGG 8 cut(s) 467, 988, 1478, 2460, 2620, 3370, 3679, 3691
PspPI GGNCC 9 cut(s) 239, 1799, 1888, 2124, 2282, 2298, 2742, 3919, 3932
PstNI CAGNNNCTG 3 cut(s) 128, 1099, 2813
PvuII CAGCTG 2 cut(s) 3563, 3980
RsaI GTAC 6 cut(s) 452, 588, 1990, 2503, 2958, 3079
RsaNI GTAC 6 cut(s) 451, 587, 1989, 2502, 2957, 3078
RseI CAYNNNNRTG 5 cut(s) 164, 573, 914, 925, 3816
SacI GAGCTC 3 cut(s) 107, 1444, 2787
SacII CCGCGG 1 cut(s) 3787
SapI GCTCTTC 1 cut(s) 802
SaqAI TTAA 8 cut(s) 320, 351, 624, 912, 923, 2411, 3036, 3486
Sau96I GGNCC 9 cut(s) 239, 1799, 1888, 2124, 2282, 2298, 2742, 3919, 3932
ScaI AGTACT 1 cut(s) 2958
SchI GAGTC 4 cut(s) 451, 965, 2328, 2812
SduI GDGCHC 9 cut(s) 107, 234, 475, 1444, 1950, 2114, 2787, 2913, 3000
SexAI ACCWGGT 1 cut(s) 2620
SfaNI GCATC 9 cut(s) 1222, 1228, 1903, 2538, 3525, 3719, 3748, 3982, 4010
SfcI CTRYAG 4 cut(s) 55, 498, 1485, 2634
Sfr274I CTCGAG 1 cut(s) 1437
Sfr303I CCGCGG 1 cut(s) 3787
SfuI TTCGAA 1 cut(s) 3295
SgrBI CCGCGG 1 cut(s) 3787
SinI GGWCC 5 cut(s) 239, 1799, 2282, 2298, 3932
SlaI CTCGAG 1 cut(s) 1437
SmiMI CAYNNNNRTG 5 cut(s) 164, 573, 914, 925, 3816
SmlI CTYRAG 7 cut(s) 439, 1373, 1437, 2177, 3537, 3714, 4026
SmoI CTYRAG 7 cut(s) 439, 1373, 1437, 2177, 3537, 3714, 4026
SnaBI TACGTA 1 cut(s) 1644
SphI GCATGC 1 cut(s) 3821
SseBI AGGCCT 1 cut(s) 2791
SspI AATATT 2 cut(s) 1896, 2409
SstI GAGCTC 3 cut(s) 107, 1444, 2787
StuI AGGCCT 1 cut(s) 2791
StyI CCWWGG 5 cut(s) 1018, 1360, 2511, 2539, 2991
TaiI ACGT 2 cut(s) 1524, 1646
TaqII GACCGA 1 cut(s) 297
TatI WGTACW 2 cut(s) 2501, 2956
TauI GCSGC 8 cut(s) 274, 537, 833, 996, 2192, 2926, 3125, 3989
Tru1I TTAA 8 cut(s) 320, 351, 624, 912, 923, 2411, 3036, 3486
Tru9I TTAA 8 cut(s) 320, 351, 624, 912, 923, 2411, 3036, 3486
TseFI GTSAC 5 cut(s) 686, 1048, 3337, 3551, 3722
Tsp45I GTSAC 5 cut(s) 686, 1048, 3337, 3551, 3722
Van91I CCANNNNNTGG 1 cut(s) 2813
VpaK11BI GGWCC 5 cut(s) 239, 1799, 2282, 2298, 3932
VspI ATTAAT 1 cut(s) 3486
XagI CCTNNNNNAGG 1 cut(s) 3216
XapI RAATTY 8 cut(s) 612, 869, 1563, 1587, 2227, 2984, 3065, 3297
XbaI TCTAGA 1 cut(s) 418
XceI RCATGY 3 cut(s) 534, 1778, 3821
XhoI CTCGAG 1 cut(s) 1437
XmiI GTMKAC 3 cut(s) 54, 2086, 2772
ZrmI AGTACT 1 cut(s) 2958
Zsp2I ATGCAT 2 cut(s) 1237, 2361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.