RLG00000024943

Regulatory-associated protein of TOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002483
Physical Location & Seq
Forward (+)
40591329 .. 40601056
9728 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024943

Sequence Viewer

Length: 4101 bp
ATGGCATTGGGGGATTTGATGGCCTCCAGGTTTTCGCAATCCTCGGTGGTCGTCGTCTCCAATCACTTCGACGACTGCGCTTCCAGCCACGACGACGGTGATGTGAGCTCGCAGAGAAGGGAATCAGAGACTGCGAGTAGTAGCTATGAGAATGCCACGGGTACAACCACTACAAGCATGGCGTACTTGCCCCAAACCATTGTCTTGTGTGAGCTTCGCCACGACGCGTTCGAGGCTCGAGTCCCTACAGGTCCTTCTGATAGCGGTTTGGTTTCCAAATGGCGGCCTAAAGACCGAATGAAGACGGGATGTGTAGCTCTAGTATTATGTTTAAACATCAGCGTTGATCCGCCCGATGTAATTAAGATATCTCCCTGTGCCAGAATGGAGTGCTGGATAGATCCATTTTCTATGGCTCCAACAAAAGCTCTTGAAACAATTGGTAAAACCTTGAGTACACAGTATGAAAGATGGCAACCAAGGGCTAAATACAGGGTTCAGCTTGATCCTACTATAGATGAAGTCAAGAAGCTTTGTAGTACATGCCGCAAATATGCCAAGTCTGAGAGAGTTCTTTTCCATTATAATGGTCATGGTGTACCCAAGCCAACTGCTAATGGTGAAATTTGGCTATTTAATAAGAGTTATACACAGTATATTCCCTTGCCAATTAGCGACCTTGATTCCTGGTTGAAGACACCTTCAATATATGTCTTTGACTGTTCTGCCGCTGGCATGATTGTTAATTCGTTCATTGAGCTCCATGATTGGGCTGGCTCAAACTCCGGATCTCCAAGAGATTGTATCCTGCTTGCTGCTTGTGAACCACACGAGACTCTTCCGCAGAGTGCGGAATTTCCTGCTGATGTCTTTACTTCTTGCCTCACAACTCCCATCAAGATGGCTTTAAGATGGTTCTGCACACGTTCCTTACTTCATGAGTCTCTTGATTATTCACTAATAGATAAAATCCCTGGCCGTCAAAATGACCGGAAAACCCTTTTGGGTGAATTGAACTGGATTTTTACTGCTATCACTGACACAATTGCATGGAATGTTCTTCCTCATGATCTTTTCCAGAGACTTTTCAGACAAGATTTGTTAGTTGCCAGCCTGTTTCGAAACTTTTTACTTGCCGAGCGGATTATGCGATCTGCAAATTGCTCTCCAATCTCTCATCCACAGTTGCCTTCTACCCATCAGCACCACATGTGGGATGCATGGGACATGGCTGCTGAGATATGCCTTTCTCAGCTTCCATTGTTGGTTGAGGATCCTAATGCTGAGTTTCAGCCAAGTCCGTTTTTCACTGAGCAGTTGACTGCTTTTGAGGTATGGCTTGACCACGGGTCTGAACACAAGAAACCACCAGAGCAGTTGCCTATTGTTCTTCAGGTCTTACTTAGTCAATGCCATCGGTTTCGAGCTCTGGTTCTTCTCGGGAGATTCCTTGATATGGGACCCTGGGCTGTAGATCTGGCATTGTCTGTTGGAATATTTCCATATGTACTGAAGCTGTTGCAAACGATGACACCTGAACTACAACAGATTCTTGTATTCATTTGGACAAAAATTCTTGCACTGGATAAGTCATGCCAGGTGGATCTAGTGAAGGATAAGGGTCATAAATATTTCATCAGGTTTCTTGATAGCATGGAAGCATATCCAGAGCAGCGTGCAATGGCTGCTTTTGTTTTGGCTGTAATAGTTGATGGCCATAGACGTGGCCAGGAAGCTTGTATTGAAGCAGGTTTAATCCATGTTTGTTTGAAGCACCTTCAGGGTCCAACACCAAATGACACACAAACTGAACCCCTATTTCTTCAGTGGCTTTGCCTCTGTCTGGGGAAACTATGGGAGGATTTCACAGATGCCCAAATATTTGGTTTGCAGGCAGATGCCCCTGCAATATGTGCTCCTCTGCTTGCTGAGCCCCAACCAGAGGTGAGGGCTTCTGCTGTTTTCGCATTGGGCACCCTGCTTGATGTGGGTTCAGGATCATGTAGAGATGGCAATGGTGGAGACGATGAGTATGATGATGAGGAAAAGATAAGGGCTGAAATTAGTATCATTAAAAGCCTATTAAGTGTTGTTTCAGATGGAAGCCCTCTTGTCAGAGCAGAAGTTGCTGTTGCTCTAGGACGCTTTGCCTTTGGACACAACAAGCACCTGAAGTCGATTGCTGCTGCCTATTGGAAACCACAGTCTAATTCTCTTGTGACTTCCTTGCCTGCTTTGGTTAGTATTAAAGGTCCTGGTAGTGCTTCATCTCATATTGGTCCACTTTTGAGAGTTGGCAATGACAGCTCATTGGTTCGGGATGGAAGAGTTTCTACCAGCAGTCCCCTGGCAAGTTCTGGAATTATGCATGGATCTCCATTGTCTGATGATTTATCTCATCATTCTGATTCTGGAATATTAGATGATGGTGGGAGCAATGGTATTGTTAACCATTTGACCCCCAAACCCCTGGACAGTCAATTGTATTCTCAGTGTGTATTGGCTATGTGTAACTTAGCCAAAGATCCATCTCCACGAATTGCAAAAATTGGTCGCCGAGTATTAGCCATTATAGGGATTGAGCAAGTGGTGGCGAAACCTGTAAAGTCCACGGGTAACAGTGTTCGACCTAGTGAATCCATTACAGTGTCTCCATGTCAGAGTCTTGCTGCTGGATTGGCTCGATCGTCTTCATGGTTCGATATGAATGGAGCTCATTTGCCTTTCCGAACTCCACCTGTCAGCCCTCCTAGGGCAAATTACTTGGCTGGAATGCGAAGAGTTTGCTCTCTAGAATTTAGGCCTCATCTAATGAGTCCAGATTCAGGTTTAGCTGATCCCCTTTTAGGTTCTGGGGGAACTTTTGGTGCCTCAGAACGAAGTTTTCTTCCTCAATCAACAATCTACAATTGGAGTTGCGGTCACTTTTCCAAGCCACTTCTTATAGCGGCAGATGACAGTAAAGAAATATTAACTAGAAGAGAGGAGAGAGAAAACTTTGCCATGGAGCACATAGCTAAATGCCGGCACTCATCTGTTAGCAAGCTTAATAATCAAATTGCAAGCTGGGACACAAAATTTGAAACGGGTACTAAAAGCATTTTGCTGGAACCATTCTCTCCTATTGTGATTGCGGCTGATGAGAATGAACGAATCAGGGTATGGAATTACCAAGAGGCTAAGGAGGCTATCCTTCTGAACAGCTTTGATAACCATGATTTTCCCGACAAAGGAATCTCCAAGCTTTGCTTTGCGAATGAACTTGATGACAGCTTGCTTCTTGCTGCCTCAAGTGATGGAAACATTCGGATTTGGAAAGATTATACTTTGAAGGGTAAACAGAAACTTGTTACTGCATTTTCTTCAATTCAAGGACATAAACCTGGTGTTCGAAGTCTTAATGCTGTTGTGGATTGGCAGCAGCAATCTGGATATCTGTATGCCTCTGGTGAGCTATCATCCATTATGCTTTGGGATCTCGATAAGGAGCAGCTTGTTAATTCTATTCATTCTCAATCAGACTGTAGCATTTCAGCATTGTCTGCATCTCAAGTTCATGGAAGCCAACTCACAGCTGGTTTTGTTGACGGTTCTGTTAGGCTTTATGATGTGCGAACTCCTGACGTGCTTGTCTGTGAAATGCGGCCGCACACTCCAAAGGTTGTAGGAAACGCACAGAAGATAGAAAGAGTTGTTGGGAACACTCAGAAGGTAGAAAGAGTTGTGGGGATTGGCTTTCAACCGGGGCTTGATCCTTCTAAGATTGTTAGTGCATGTCAGGCTGGTGACATTCAGTTCCTTGATATCAGAAATAGTAAAGATCCCTACCTCACAATCGAAGCTCACAGGGGTTCACTCACAGCTTTAGCTGTTCATAGACACGCTCCGCTCATTGCCAGTGGCTCAGCCAAGCAGCTCATAAAAGTCTTTAGTTTGGAGGGCGAACAATTAGGCACCATTAGATACTACCCTTCCTTTATGGCCCAAAAAATTGGTCCTGTTAGCTGCCTAGCCTTCCATCCGTATGAAGTACTGCTTGCTGCTGGTGCTGCAGATGCTTGCGCGTCCATCTATGCCGATGACAACTCTCAAGGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0001558 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008361 GO:0009267 GO:0009605 GO:0009719 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009893 GO:0009894 GO:0009987 GO:0009991 GO:0010033 GO:0010073 GO:0010074 GO:0010154 GO:0010243 GO:0010492 GO:0010506 GO:0010562 GO:0010604 GO:0016043 GO:0019220 GO:0019222 GO:0019827 GO:0022414 GO:0023052 GO:0030307 GO:0030674 GO:0031323 GO:0031325 GO:0031329 GO:0031399 GO:0031401 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032535 GO:0032991 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040008 GO:0042221 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043200 GO:0043549 GO:0044093 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045927 GO:0045937 GO:0048316 GO:0048507 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060090 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071840 GO:0071900 GO:0071902 GO:0080008 GO:0080090 GO:0090066 GO:0098727 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04714 ko04910 ko05206 map04136 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04714 map04910 map05206
Pfam Domains
Protein Families
HEAT Raptor_N WD40

Protein Analysis

1367

Amino Acids

150.26

Weight (kDa)

6.05

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raptor_N PF14538 100 - 251 1.4e-70 Raptor N-terminal CASPase like domain
WD40_WDHD1_1st PF24817 1030 - 1181 9.7e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 1042 - 1210 1.8e-12 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 1043 - 1232 3.2e-07 TEP-1 second beta-propeller
WD40_Prp19 PF24814 1051 - 1217 1.5e-09 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 1135 - 1223 7.6e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 1147 - 1358 3.1e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 1240 - 1357 3.6e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 1252 - 1357 6.2e-06 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AasI GACNNNNNNGTC 1 cut(s) 3629
Acc36I ACCTGC 1 cut(s) 1739
AccB1I GGYRCC 3 cut(s) 1973, 2867, 3953
AccBSI CCGCTC 2 cut(s) 1141, 3889
AccII CGCG 2 cut(s) 227, 4064
AccIII TCCGGA 1 cut(s) 785
AcoI YGGCCR 4 cut(s) 976, 1714, 1726, 3644
AcsI RAATTY 5 cut(s) 624, 854, 1572, 2795, 3077
AcuI CTGAAG 5 cut(s) 1376, 1532, 1763, 1808, 2192
AfaI GTAC 8 cut(s) 163, 185, 457, 541, 600, 1509, 3091, 4032
AflIII ACRYGT 3 cut(s) 225, 923, 1209
AhdI GACNNNNNGTC 1 cut(s) 1348
AjiI CACGTC 2 cut(s) 1724, 3625
AjuI GAANNNNNNNTTGG 4 cut(s) 986, 1018, 3678, 3710
Alw21I GWGCWC 6 cut(s) 110, 762, 1429, 1918, 2716, 3012
Alw26I GTCTC 7 cut(s) 61, 122, 827, 948, 1075, 2016, 2655
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 2 cut(s) 237, 1439
Aor13HI TCCGGA 1 cut(s) 785
AoxI GGCC 8 cut(s) 21, 284, 976, 1714, 1726, 2801, 3644, 3981
ApoI RAATTY 5 cut(s) 624, 854, 1572, 2795, 3077
ArsI GACNNNNNNTTYG 4 cut(s) 3062, 3094, 3979, 4011
Asp700I GAANNNNTTC 1 cut(s) 2329
AspA2I CCTAGG 1 cut(s) 2750
AspLEI GCGC 2 cut(s) 80, 4064
AspS9I GGNCC 7 cut(s) 251, 1460, 1784, 2252, 2279, 3982, 3995
AsuC2I CCSGG 1 cut(s) 3744
AsuHPI GGTGA 6 cut(s) 110, 632, 1019, 1957, 3461, 3797
AsuII TTCGAA 2 cut(s) 1120, 3391
AvaI CYCGRG 2 cut(s) 237, 1439
AvaII GGWCC 6 cut(s) 251, 1460, 1784, 2252, 2279, 3995
AvrII CCTAGG 1 cut(s) 2750
BaeGI GKGCMC 1 cut(s) 1976
BalI TGGCCA 2 cut(s) 1716, 1728
BamHI GGATCC 1 cut(s) 1273
BanI GGYRCC 3 cut(s) 1973, 2867, 3953
BanII GRGCYC 5 cut(s) 110, 762, 1429, 1935, 2716
BarI GAAGNNNNNNTAC 2 cut(s) 2317, 2349
BauI CACGAG 1 cut(s) 830
BbsI GAAGAC 3 cut(s) 308, 701, 2682
Bbv12I GWGCWC 6 cut(s) 110, 762, 1429, 1918, 2716, 3012
BceAI ACGGC 1 cut(s) 963
BcgI CGANNNNNNTGC 2 cut(s) 2766, 2800
BciVI GTATCC 1 cut(s) 815
BcnI CCSGG 1 cut(s) 3744
BcoDI GTCTC 7 cut(s) 61, 122, 827, 948, 1075, 2016, 2655
BfaI CTAG 8 cut(s) 320, 1607, 2138, 2631, 2751, 2792, 2976, 4010
BfmI CTRYAG 5 cut(s) 246, 513, 1470, 3523, 4050
BfuAI ACCTGC 1 cut(s) 1739
BfuI GTATCC 1 cut(s) 815
BglII AGATCT 1 cut(s) 1474
BlnI CCTAGG 1 cut(s) 2750
BlpI GCTNAGC 2 cut(s) 1929, 3904
BmcAI AGTACT 1 cut(s) 4032
Bme18I GGWCC 6 cut(s) 251, 1460, 1784, 2252, 2279, 3995
BmeRI GACNNNNNGTC 1 cut(s) 1348
BmeT110I CYCGRG 2 cut(s) 237, 1439
BmgBI CACGTC 2 cut(s) 1724, 3625
BmgT120I GGNCC 7 cut(s) 251, 1460, 1784, 2252, 2279, 3982, 3995
BmiI GGNNCC 9 cut(s) 417, 1275, 1461, 1462, 1785, 1975, 2869, 3111, 3955
BmsI GCATC 5 cut(s) 1207, 1861, 1888, 3554, 4045
BpiI GAAGAC 3 cut(s) 308, 701, 2682
BpmI CTGGAG 1 cut(s) 10
Bpu10I CCTNAGC 1 cut(s) 3180
Bpu1102I GCTNAGC 2 cut(s) 1929, 3904
Bpu14I TTCGAA 2 cut(s) 1120, 3391
BpuEI CTTGAG 4 cut(s) 472, 3274, 3534, 4074
BpuMI CCSGG 1 cut(s) 3744
BsaWI WCCGGW 2 cut(s) 785, 990
BsaXI ACNNNNNCTCC 6 cut(s) 2425, 2455, 2635, 2665, 2905, 2935
Bse118I RCCGGY 1 cut(s) 3024
Bse1I ACTGG 3 cut(s) 1022, 1587, 3897
Bse3DI GCAATG 5 cut(s) 1686, 2020, 2305, 2443, 3891
BseAI TCCGGA 1 cut(s) 785
BseGI GGATG 6 cut(s) 314, 1177, 1222, 2326, 3458, 4018
BseMI GCAATG 5 cut(s) 1686, 2020, 2305, 2443, 3891
BseNI ACTGG 3 cut(s) 1022, 1587, 3897
BseRI GAGGAG 2 cut(s) 1908, 2999
BseSI GKGCMC 1 cut(s) 1976
BseX3I CGGCCG 1 cut(s) 3644
BseYI CCCAGC 1 cut(s) 3066
BsgI GTGCAG 1 cut(s) 904
Bsh1236I CGCG 2 cut(s) 227, 4064
Bsh1285I CGRYCG 2 cut(s) 2687, 3647
BshFI GGCC 8 cut(s) 23, 286, 978, 1716, 1728, 2803, 3646, 3983
BshNI GGYRCC 3 cut(s) 1973, 2867, 3953
BsiEI CGRYCG 2 cut(s) 2687, 3647
BsiHKAI GWGCWC 6 cut(s) 110, 762, 1429, 1918, 2716, 3012
BsiHKCI CYCGRG 2 cut(s) 237, 1439
BsiSI CCGG 4 cut(s) 786, 991, 3025, 3743
BslFI GGGAC 5 cut(s) 227, 1238, 1473, 2328, 3083
BsmAI GTCTC 7 cut(s) 61, 122, 827, 948, 1075, 2016, 2655
BsmBI CGTCTC 2 cut(s) 61, 2016
BsmFI GGGAC 5 cut(s) 227, 1238, 1473, 2328, 3083
BsmI GAATGC 2 cut(s) 157, 2778
BsnI GGCC 8 cut(s) 23, 286, 978, 1716, 1728, 2803, 3646, 3983
BsoBI CYCGRG 2 cut(s) 237, 1439
Bsp119I TTCGAA 2 cut(s) 1120, 3391
Bsp1286I GDGCHC 8 cut(s) 110, 762, 1429, 1918, 1935, 1976, 2716, 3012
Bsp13I TCCGGA 1 cut(s) 785
Bsp1720I GCTNAGC 2 cut(s) 1929, 3904
Bsp19I CCATGG 1 cut(s) 3003
BspANI GGCC 8 cut(s) 23, 286, 978, 1716, 1728, 2803, 3646, 3983
BspEI TCCGGA 1 cut(s) 785
BspFNI CGCG 2 cut(s) 227, 4064
BspHI TCATGA 2 cut(s) 937, 1066
BspLI GGNNCC 9 cut(s) 417, 1275, 1461, 1462, 1785, 1975, 2869, 3111, 3955
BspMAI CTGCAG 1 cut(s) 4054
BspMI ACCTGC 1 cut(s) 1739
BspT104I TTCGAA 2 cut(s) 1120, 3391
BspT107I GGYRCC 3 cut(s) 1973, 2867, 3953
BsrBI CCGCTC 2 cut(s) 1141, 3889
BsrDI GCAATG 5 cut(s) 1686, 2020, 2305, 2443, 3891
BsrFI RCCGGY 1 cut(s) 3024
BsrI ACTGG 3 cut(s) 1022, 1587, 3897
BssAI RCCGGY 1 cut(s) 3024
BssSI CACGAG 1 cut(s) 830
BssT1I CCWWGG 3 cut(s) 479, 2750, 3003
Bst2BI CACGAG 1 cut(s) 830
Bst6I CTCTTC 4 cut(s) 843, 2320, 2773, 2974
BstAPI GCANNNNNTGC 4 cut(s) 1685, 1913, 2126, 3542
BstBI TTCGAA 2 cut(s) 1120, 3391
BstDSI CCRYGG 4 cut(s) 156, 1345, 2610, 3003
BstF5I GGATG 6 cut(s) 314, 1177, 1222, 2326, 3458, 4018
BstFNI CGCG 2 cut(s) 227, 4064
BstHHI GCGC 2 cut(s) 80, 4064
BstMAI GTCTC 7 cut(s) 61, 122, 827, 948, 1075, 2016, 2655
BstMCI CGRYCG 2 cut(s) 2687, 3647
BstNSI RCATGY 3 cut(s) 546, 1213, 3777
BstSFI CTRYAG 5 cut(s) 246, 513, 1470, 3523, 4050
BstSLI GKGCMC 1 cut(s) 1976
BstUI CGCG 2 cut(s) 227, 4064
BstV2I GAAGAC 3 cut(s) 308, 701, 2682
BstX2I RGATCY 9 cut(s) 400, 788, 1273, 1474, 1603, 2372, 2524, 3475, 3820
BstXI CCANNNNNNTGG 6 cut(s) 587, 901, 1724, 1883, 2470, 3992
BstYI RGATCY 9 cut(s) 400, 788, 1273, 1474, 1603, 2372, 2524, 3475, 3820
BstZI CGGCCG 1 cut(s) 3644
BsuI GTATCC 1 cut(s) 815
BsuRI GGCC 8 cut(s) 23, 286, 978, 1716, 1728, 2803, 3646, 3983
BtgI CCRYGG 4 cut(s) 156, 1345, 2610, 3003
BtrI CACGTC 2 cut(s) 1724, 3625
BtsCI GGATG 6 cut(s) 314, 1177, 1222, 2326, 3458, 4018
BtsIMutI CAGTG 8 cut(s) 1035, 1308, 1580, 1832, 2498, 2626, 2652, 3904
BveI ACCTGC 1 cut(s) 1739
CaiI CAGNNNCTG 1 cut(s) 131
CciI TCATGA 2 cut(s) 937, 1066
CciNI GCGGCCGC 1 cut(s) 3644
CfoI GCGC 2 cut(s) 80, 4064
Cfr10I RCCGGY 1 cut(s) 3024
Cfr13I GGNCC 7 cut(s) 251, 1460, 1784, 2252, 2279, 3982, 3995
CseI GACGC 3 cut(s) 233, 2151, 4053
CsiI ACCWGGT 1 cut(s) 3382
Csp6I GTAC 8 cut(s) 162, 184, 456, 540, 599, 1508, 3090, 4031
CviQI GTAC 8 cut(s) 162, 184, 456, 540, 599, 1508, 3090, 4031
DraI TTTAAA 1 cut(s) 333
DrdI GACNNNNNNGTC 1 cut(s) 3629
DriI GACNNNNNGTC 1 cut(s) 1348
DseDI GACNNNNNNGTC 1 cut(s) 3629
EaeI YGGCCR 4 cut(s) 976, 1714, 1726, 3644
EagI CGGCCG 1 cut(s) 3644
Eam1104I CTCTTC 4 cut(s) 843, 2320, 2773, 2974
Eam1105I GACNNNNNGTC 1 cut(s) 1348
EarI CTCTTC 4 cut(s) 843, 2320, 2773, 2974
EciI GGCGGA 1 cut(s) 339
Ecl136II GAGCTC 4 cut(s) 108, 760, 1427, 2714
EclXI CGGCCG 1 cut(s) 3644
Eco130I CCWWGG 3 cut(s) 479, 2750, 3003
Eco147I AGGCCT 1 cut(s) 2803
Eco24I GRGCYC 5 cut(s) 110, 762, 1429, 1935, 2716
Eco32I GATATC 3 cut(s) 369, 3434, 3805
Eco47I GGWCC 6 cut(s) 251, 1460, 1784, 2252, 2279, 3995
Eco52I CGGCCG 1 cut(s) 3644
Eco53kI GAGCTC 4 cut(s) 108, 760, 1427, 2714
Eco57I CTGAAG 5 cut(s) 1376, 1532, 1763, 1808, 2192
Eco88I CYCGRG 2 cut(s) 237, 1439
EcoICRI GAGCTC 4 cut(s) 108, 760, 1427, 2714
EcoO109I RGGNCCY 3 cut(s) 251, 1460, 2252
EcoRV GATATC 3 cut(s) 369, 3434, 3805
EcoT14I CCWWGG 3 cut(s) 479, 2750, 3003
EcoT22I ATGCAT 2 cut(s) 1222, 2370
EcoT38I GRGCYC 5 cut(s) 110, 762, 1429, 1935, 2716
ErhI CCWWGG 3 cut(s) 479, 2750, 3003
Esp3I CGTCTC 2 cut(s) 61, 2016
FalI AAGNNNNNCTT 4 cut(s) 3233, 3265, 4020, 4052
FaqI GGGAC 5 cut(s) 227, 1238, 1473, 2328, 3083
FauNDI CATATG 1 cut(s) 1504
FokI GGATG 6 cut(s) 321, 1164, 1229, 2333, 3445, 4005
FriOI GRGCYC 5 cut(s) 110, 762, 1429, 1935, 2716
FspBI CTAG 8 cut(s) 320, 1607, 2138, 2631, 2751, 2792, 2976, 4010
GlaI GCGC 2 cut(s) 79, 4063
GsaI CCCAGC 1 cut(s) 3070
GsuI CTGGAG 1 cut(s) 10
HaeIII GGCC 8 cut(s) 23, 286, 978, 1716, 1728, 2803, 3646, 3983
HapII CCGG 4 cut(s) 786, 991, 3025, 3743
HgaI GACGC 3 cut(s) 233, 2151, 4053
HhaI GCGC 2 cut(s) 80, 4064
Hin6I GCGC 2 cut(s) 78, 4062
HinP1I GCGC 2 cut(s) 78, 4062
HincII GTYRAC 3 cut(s) 1320, 2449, 3586
HindII GTYRAC 3 cut(s) 1320, 2449, 3586
HindIII AAGCTT 4 cut(s) 530, 1734, 3044, 3242
HpaI GTTAAC 1 cut(s) 2449
HpaII CCGG 4 cut(s) 786, 991, 3025, 3743
HphI GGTGA 6 cut(s) 110, 632, 1019, 1957, 3461, 3797
Hpy99I CGWCG 5 cut(s) 56, 74, 95, 98, 227
HpyCH4IV ACGT 3 cut(s) 925, 1723, 3624
HpySE526I ACGT 3 cut(s) 925, 1723, 3624
HspAI GCGC 2 cut(s) 78, 4062
KflI GGGWCCC 1 cut(s) 1460
Kpn2I TCCGGA 1 cut(s) 785
KroI GCCGGC 1 cut(s) 3024
KroNI GCCGGC 1 cut(s) 3026
KspAI GTTAAC 1 cut(s) 2449
LmnI GCTCC 8 cut(s) 421, 765, 1921, 2433, 2711, 3007, 3487, 3889
LweI GCATC 5 cut(s) 1207, 1861, 1888, 3554, 4045
MabI ACCWGGT 1 cut(s) 3382
MaeI CTAG 8 cut(s) 320, 1607, 2138, 2631, 2751, 2792, 2976, 4010
MaeII ACGT 3 cut(s) 925, 1723, 3624
MaeIII GTNAC 6 cut(s) 2218, 2510, 2615, 2921, 3349, 3785
MbiI CCGCTC 2 cut(s) 1141, 3889
MfeI CAATTG 4 cut(s) 438, 1044, 2480, 2908
MflI RGATCY 9 cut(s) 400, 788, 1273, 1474, 1603, 2372, 2524, 3475, 3820
MhlI GDGCHC 8 cut(s) 110, 762, 1429, 1918, 1935, 1976, 2716, 3012
MlsI TGGCCA 2 cut(s) 1716, 1728
MluI ACGCGT 1 cut(s) 225
MluNI TGGCCA 2 cut(s) 1716, 1728
MlyI GAGTC 5 cut(s) 249, 829, 950, 2671, 2824
MmeI TCCRAC 3 cut(s) 443, 1471, 1811
Mox20I TGGCCA 2 cut(s) 1716, 1728
Mph1103I ATGCAT 2 cut(s) 1222, 2370
MroI TCCGGA 1 cut(s) 785
MroNI GCCGGC 1 cut(s) 3024
MroXI GAANNNNTTC 1 cut(s) 2329
MscI TGGCCA 2 cut(s) 1716, 1728
MslI CAYNNNNRTG 5 cut(s) 585, 899, 1722, 2645, 4023
Msp20I TGGCCA 2 cut(s) 1716, 1728
MspA1I CMGCKG 2 cut(s) 731, 3575
MspI CCGG 4 cut(s) 786, 991, 3025, 3743
MssI GTTTAAAC 1 cut(s) 333
MunI CAATTG 4 cut(s) 438, 1044, 2480, 2908
Mva1269I GAATGC 2 cut(s) 157, 2778
MvnI CGCG 2 cut(s) 227, 4064
NaeI GCCGGC 1 cut(s) 3026
NciI CCSGG 1 cut(s) 3744
NcoI CCATGG 1 cut(s) 3003
NdeI CATATG 1 cut(s) 1504
NgoMIV GCCGGC 1 cut(s) 3024
NlaIV GGNNCC 9 cut(s) 417, 1275, 1461, 1462, 1785, 1975, 2869, 3111, 3955
NmeAIII GCCGAG 2 cut(s) 1162, 2582
NmuCI GTSAC 3 cut(s) 2218, 2921, 3785
NotI GCGGCCGC 1 cut(s) 3644
NsiI ATGCAT 2 cut(s) 1222, 2370
NspI RCATGY 3 cut(s) 546, 1213, 3777
NspV TTCGAA 2 cut(s) 1120, 3391
PaeR7I CTCGAG 1 cut(s) 237
PagI TCATGA 2 cut(s) 937, 1066
PasI CCCWGGG 1 cut(s) 1464
PceI AGGCCT 1 cut(s) 2803
PciI ACATGT 1 cut(s) 1209
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PctI GAATGC 2 cut(s) 157, 2778
PdiI GCCGGC 1 cut(s) 3026
PdmI GAANNNNTTC 1 cut(s) 2329
PfeI GAWTC 9 cut(s) 122, 683, 1446, 1549, 2408, 2636, 2822, 3153, 3234
Ple19I CGATCG 1 cut(s) 2687
PleI GAGTC 5 cut(s) 248, 829, 949, 2670, 2823
PmeI GTTTAAAC 1 cut(s) 333
PpsI GAGTC 5 cut(s) 248, 829, 949, 2670, 2823
PpuMI RGGWCCY 3 cut(s) 251, 1460, 2252
PscI ACATGT 1 cut(s) 1209
PsiI TTATAA 1 cut(s) 585
Psp124BI GAGCTC 4 cut(s) 110, 762, 1429, 2716
Psp5II RGGWCCY 3 cut(s) 251, 1460, 2252
PspFI CCCAGC 1 cut(s) 3066
PspN4I GGNNCC 9 cut(s) 417, 1275, 1461, 1462, 1785, 1975, 2869, 3111, 3955
PspPI GGNCC 7 cut(s) 251, 1460, 1784, 2252, 2279, 3982, 3995
PspPPI RGGWCCY 3 cut(s) 251, 1460, 2252
PspXI VCTCGAGB 1 cut(s) 237
PstI CTGCAG 1 cut(s) 4054
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 9 cut(s) 400, 788, 1273, 1474, 1603, 2372, 2524, 3475, 3820
PvuI CGATCG 1 cut(s) 2687
PvuII CAGCTG 1 cut(s) 3575
RsaI GTAC 8 cut(s) 163, 185, 457, 541, 600, 1509, 3091, 4032
RsaNI GTAC 8 cut(s) 162, 184, 456, 540, 599, 1508, 3090, 4031
RseI CAYNNNNRTG 5 cut(s) 585, 899, 1722, 2645, 4023
SacI GAGCTC 4 cut(s) 110, 762, 1429, 2716
Sau96I GGNCC 7 cut(s) 251, 1460, 1784, 2252, 2279, 3982, 3995
ScaI AGTACT 1 cut(s) 4032
SchI GAGTC 5 cut(s) 249, 829, 950, 2671, 2824
SduI GDGCHC 8 cut(s) 110, 762, 1429, 1918, 1935, 1976, 2716, 3012
SexAI ACCWGGT 1 cut(s) 3382
SfaNI GCATC 5 cut(s) 1207, 1861, 1888, 3554, 4045
SfcI CTRYAG 5 cut(s) 246, 513, 1470, 3523, 4050
Sfr274I CTCGAG 1 cut(s) 237
SfuI TTCGAA 2 cut(s) 1120, 3391
SinI GGWCC 6 cut(s) 251, 1460, 1784, 2252, 2279, 3995
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 5 cut(s) 585, 899, 1722, 2645, 4023
SmlI CTYRAG 5 cut(s) 237, 451, 3289, 3549, 4089
SmoI CTYRAG 5 cut(s) 237, 451, 3289, 3549, 4089
SseBI AGGCCT 1 cut(s) 2803
SspI AATATT 5 cut(s) 1497, 1631, 1881, 2418, 2970
SspMI CTAG 8 cut(s) 320, 1607, 2138, 2631, 2751, 2792, 2976, 4010
SstI GAGCTC 4 cut(s) 110, 762, 1429, 2716
StuI AGGCCT 1 cut(s) 2803
StyI CCWWGG 3 cut(s) 479, 2750, 3003
TaiI ACGT 3 cut(s) 928, 1726, 3627
TaqII GACCGA 1 cut(s) 309
TatI WGTACW 4 cut(s) 455, 539, 1507, 4030
TauI GCSGC 7 cut(s) 286, 549, 731, 2951, 3137, 3646, 3649
TfiI GAWTC 9 cut(s) 122, 683, 1446, 1549, 2408, 2636, 2822, 3153, 3234
TscAI CASTG 8 cut(s) 1042, 1315, 1587, 1832, 2498, 2626, 2652, 3904
TseFI GTSAC 3 cut(s) 2218, 2921, 3785
Tsp45I GTSAC 3 cut(s) 2218, 2921, 3785
TspGWI ACGGA 2 cut(s) 1290, 4011
TspRI CASTG 8 cut(s) 1042, 1315, 1587, 1832, 2498, 2626, 2652, 3904
VpaK11BI GGWCC 6 cut(s) 251, 1460, 1784, 2252, 2279, 3995
XapI RAATTY 5 cut(s) 624, 854, 1572, 2795, 3077
XbaI TCTAGA 1 cut(s) 2791
XceI RCATGY 3 cut(s) 546, 1213, 3777
XcmI CCANNNNNNNNNTGG 4 cut(s) 175, 770, 2344, 3572
XhoI CTCGAG 1 cut(s) 237
XmaJI CCTAGG 1 cut(s) 2750
XmnI GAANNNNTTC 1 cut(s) 2329
XspI CTAG 8 cut(s) 320, 1607, 2138, 2631, 2751, 2792, 2976, 4010
ZrmI AGTACT 1 cut(s) 4032
Zsp2I ATGCAT 2 cut(s) 1222, 2370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.