AT5G01770

Regulatory-associated protein of TOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002483
Physical Location & Seq
Reverse (-)
293675 .. 302015
8341 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01770.4

Sequence Viewer

Length: 4011 bp
ATGGCATTAGGGGACTTGATGGTCTCCAGGCTCTCTCAATCTTCCGTTACTGTTGTCACCAATCACCTCTACGACGACGACGACAACTGCGCCTCCTCCGCTCACGATGATTCCCGTGTGTCCATCATCGCCTCTCCCAGAGTCGCAAGTAGCAGCTATGAGAACCTCTCCGCCGCCACAAGCATGGCATACTTGCCTCAGACTCTTGTGCTCTGTGACCTTAGACACGACGACGCATCCGACATCGTCCAACCCCCAAGATGGCGCCTTAAAGAGCGAATGAAAACAGGATGTGTTGCTCTAGTTATGTGTTTGCACATTACTGTTGATCCCCCTGATGTTATTAAGATTTCTCCCTGCGCAAGACTCGAATGCTGGATAGATCCTTTTTCTATGTTTCCCCCAAGAAGAGCTCTTGAGGCTATTGGCCAAAACTTGAGCATTCAGTACGAGAGATGGCTGGCTCGGGCTCGATATAAAGTCGAATTGGATCCTACAAAGGACGATGTCAGGAAGCTCTGCTTGTCTTGCCGGAAATATGCAAAGACTGAGAGAGTTTTGTTTCACTATAACGGGCATGGTGTCCCTAAACCTACACCTAATGGTGAAATCTGGGTTTACAATAAGAATTTTACGCAGTACATTCCCTTGCCAGTCAGCGAGCTTGATTCCTGGTTGAAAACACCCACCATTTACGTTTTTGACTGCTCTGCTGCTAGGGTGATCCTCAATGCCTTCGCCGAGGGGGAATCTTCTGGACCCCCGAAGGACTGTATTCTACTGGCTGCTTGTGATGTACATGAGACACTTCCTCAGAGTGTTGAATTTCCAGCTGACGTTTTTACTTCTTGCCTCACAACACCTATTAATATCGCATTGAAATGGTTCTGCAGACGTTCTCTTCTGAAAGAATTTATAGATGAATCACTTATTGATAGAATTCCCGGTCGCCAAAATGACCGTAAAACATTGTTAGGAGAGTTGAACTGGATCTTCACTGCAGTCACAGACACCATTGCCTGGAATGTGCTTCCACGCGAACTTTTCCAGAGATTATTCAGACAGGACTTGTTGGTTGCTAGCCTTTTCCGAAATTTCTTACTAGCTGAGAGGATAATGCGGTCCGGAAATTGTACCCCAATATCACATCCTATGCTACCTCCTACGCATCAGCATCATATGTGGGATGCATGGGACATGGCTGCTGAAATCTGCCTTTCTCAGCTTCCTCAATTTTTTCTGGACCCAAATACGGAGTTTCAGCCAAGTTCATTTTTCACTGAGCAACTGACAGCTTTTGAGGTCTGGCTGGATCATGGATCTGAGCATAAGAAGCCACCAGAGCAGTTACCTATTGTTCTTCAGGTCTTACTTAGCCAGTGCCATCGGTATCGTGCTCTGGTGCTTCTTGGAAGATTTCTTGATATGGGTCCTTGGGCTGTCGATTTGGCCTTATCTGTTGGAATATATCCTTGCGTAGTGAAGCTGCTGCAAACAACAACTATTGAGCTACGGCAGATTCTTGTTTTCATATGGACAAAGATCCTAGCACTTGATAAGTCCTGTCAAGTTGATCTTGTGAAGGACCGGGGCCACATATATTTCATTCGATTTTTAGATAGTTCTGATGCATTCCCAGAACAACGTGCTATGGCTGCGTTTATTTTGGCTGTTATTGTCGATGGCTACAAACGAGGCCAAGAATCATGTCTTGAAGCTAATTTAATTGCTGTTTGTCTGGGGCACCTCGAAGCAACCCAACTATGTGATCCACCGCCAGAACCATTGTTTCTACAATGGCTTTGTCTTTGTCTTGGAAAGTTGTGGGAGGACTATTTGGAGGCCCAGATAATGGGTAGGGAGGCAAATGCTTCTGAGAATCTAATTGCTGGCCACACCAATCTCTTGCAGGTCAGGGCTGCTGCTGTTTTTGCCTTGGGTACCTTACTTGATGTTGGGTTTGACTCTGGTAAAGGTGTTTGTGATGAAGAATTTGATGATGATGAAAATATAGTTGAAGATATTATCATAAAAAGTCTCTTAGATGTAGTTTCAGATGGGAGCCCGCTTGTCCGAACAGAGGTTGCTGTAGCTCTCGCACGGTTTGCTTTTGGACACAAACAACATCTAAAGTCAGTTGCAGATTCATACTGGAAGCCCCAAAATTCTCTGCGAACTTCTCTCCCTTCAATGGCCAAGTTTCATGACTCTGGGACTTCAATAGTTGCTTCTTCAGACATGGGTTCTCTGACTAGAGCTAGCCCTGATAGCCAACCAGTGGCTCGTGAAGGAAGGATCTCAAGCAGCCTCCAAGAACCTTTTTCCGGGCTAATGCAAGGATCTCCATTAGCTGATGACTCCTCCTTGCATTCTGATGTTGGAATCATCCATGATGGTGTCAGCAATGGAGTTGTCCATCAGCCAAGACCGCTGGATAATGCTATCTATTCGCAGAGTGTTCTAGCTATGTTTACCTTAGCTAAAGATCCATCTCCACGTATTGCAAGCCTTGGGCGGCGTGTGCTTTCTGTTATTGGGATTGAACAAATTGTTGCGAAACCCTCCAAGTCCAATGGCCGACCAGGTGAAGCAGCATCAGCATCTCACACTCCTCTGGCTGGCCTAGTTCGCTCATCCTCTTGGTTTGACATGCATACAGGCCACCTACCGTTAACATTTAGGACTCCTCCGGTAAGCCCTCCGCAAACAAGCTATTTAACTGGACTGAGGAGAGTTTGTTCGCTAGAGCTCCGACCTCATCTGCTGGGTTCTCCCGACTCTGGATTGGCTGATCCGATTTTAGGTGTAAGCGGGTCTGAACGGAGTTTGCTTCCACAATCAACTATCTACAACTGGAGCTGTGGTCACTTTTCCAAACCTCTTCTTGGCGGAGCAGATGCTAATGAAGAGATAGCAGCCCAAAGAGAAGAAAAAAAAAAGTTTTCGCTGGAGCATATTGCAAAATGCCAGCATTCATCAATAAGCGGGCTCAGCAATATTCCGATTGCTAATTGGGATACAAAGTTTGAAACGGGAACAAAGACGGCCCTCCTTCACCCTTTTTCTCCTATTGTAGTTGCTGCCGATGAGAATGAACGGATTAGGGTGTGGAATTACGAGGAAGCAACTCTTCTTAATGGCTTTGACAATAACGATTTTCCTGACAAAGGAATTTCAAATCTCTGCCTTGTCAATGAACTTGATGACTCTCTGCTACTTGTTGCCTCTTGTAATGTTCCAACTCTTTCCAGAGCGTCTTTTGCAATCCGTATATGGAAAGACTACGCAACAAAGGGTAGACAAAAACTTGTCACAGGGTTTTCGTCAATCCAAGGTCAGAAGCCTGGTGCAAGTGGCCTGAACGCTGTCGTGGACTGGCAACAACAGTCAGGTTACCTGTATGTTTCTGGGGAATCGTTATCAATCATGGTTTGGGACCTGGATAAAGAACAGCTCGTCAAATCTATGCCCTTTGAATCCGGGTGCAGCGTCACAGCCCTTTCGGCTTCTCAAGTGCATGGGAGTCAACTTGCTGCTGGTTTTGCGGATGGATCTGTAAGACTCTACGACGTTCGCACACCTGACTTTCTTGTGTGCGCCACTCGGCCTCATCAGCGAGTAGAAAAAGTAGTTGGACTCAGCTTTCAGCCTGGACTCGATCCAGCAAAGATTGTGAGCGCATCACAAGCAGGTGACATTCAGTTTCTGGATCTGAGAAGACCAAAGGAGACATACCTGACTATAGACGCGCATAGAGGATCACTGACAGCATTGGGTGTTCATAGACATGCTCCAATCATAGCGAGCGGGTCAGCAAAACAGCTAATAAAAGTGTTCAGCCTTAAAGGAGAGCAACTTGGGATCATAAAGTACCACACTTCCTTCATGGGCCAACAGATTGGCCCAGTCAGTTGCCTCGCCTTTCACCCTTACCAGATGTTGTTAGCCGCAGGGGCTGCTGGCTCCTTCGTCTCCTTATACACCCACCACAACACACAATTACCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0001558 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008361 GO:0009267 GO:0009605 GO:0009719 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009893 GO:0009894 GO:0009987 GO:0009991 GO:0010033 GO:0010073 GO:0010074 GO:0010154 GO:0010243 GO:0010492 GO:0010506 GO:0010562 GO:0010604 GO:0016043 GO:0019220 GO:0019222 GO:0019827 GO:0022414 GO:0023052 GO:0030307 GO:0030674 GO:0031323 GO:0031325 GO:0031329 GO:0031399 GO:0031401 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032535 GO:0032991 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040008 GO:0042221 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043200 GO:0043549 GO:0044093 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045927 GO:0045937 GO:0048316 GO:0048507 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060090 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071840 GO:0071900 GO:0071902 GO:0080008 GO:0080090 GO:0090066 GO:0098727 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04714 ko04910 ko05206 map04136 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04714 map04910 map05206
Pfam Domains
Protein Families
HEAT Raptor_N WD40

Protein Analysis

1336

Amino Acids

147.64

Weight (kDa)

6.04

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raptor_N PF14538 94 - 246 3.1e-65 Raptor N-terminal CASPase like domain
WD40_WDHD1_1st PF24817 1020 - 1213 1.8e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 1027 - 1239 1.2e-09 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 1118 - 1327 1.2e-10 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 1118 - 1314 8.4e-14 CDC20/Fizzy WD40 domain
WDR55 PF24796 1118 - 1276 5.8e-06 WDR55
Beta-prop_THOC3 PF25174 1119 - 1325 5.9e-14 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 1132 - 1322 2.8e-14 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 1136 - 1273 1.5e-09 WDR36/Utp21 first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3680
AasI GACNNNNNNGTC 1 cut(s) 20
Acc16I TGCGCA 1 cut(s) 361
Acc36I ACCTGC 2 cut(s) 1900, 3680
Acc65I GGTACC 1 cut(s) 1940
AccB1I GGYRCC 3 cut(s) 264, 1743, 1940
AccB7I CCANNNNNTGG 3 cut(s) 1020, 1852, 2278
AccBSI CCGCTC 2 cut(s) 101, 3807
AccI GTMKAC 1 cut(s) 3298
AccII CGCG 2 cut(s) 1038, 3749
AccIII TCCGGA 1 cut(s) 1124
AcoI YGGCCR 4 cut(s) 427, 1891, 2193, 2575
AcsI RAATTY 8 cut(s) 628, 824, 911, 939, 1093, 1991, 2164, 3171
AcuI CTGAAG 2 cut(s) 1346, 2217
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 6 cut(s) 449, 641, 798, 1135, 1942, 3872
AjnI CCWGG 7 cut(s) 26, 671, 1019, 2581, 3343, 3438, 3649
AjuI GAANNNNNNNTTGG 2 cut(s) 3615, 3647
AloI GAACNNNNNNTCC 2 cut(s) 2722, 2754
Alw21I GWGCWC 4 cut(s) 213, 415, 1399, 2751
Alw26I GTCTC 5 cut(s) 28, 797, 2042, 3722, 3976
AlwNI CAGNNNCTG 2 cut(s) 3706, 3956
Ama87I CYCGRG 1 cut(s) 465
Aor13HI TCCGGA 1 cut(s) 1124
ApoI RAATTY 8 cut(s) 628, 824, 911, 939, 1093, 1991, 2164, 3171
AseI ATTAAT 1 cut(s) 867
Asp700I GAANNNNTTC 1 cut(s) 884
Asp718I GGTACC 1 cut(s) 1940
AspLEI GCGC 6 cut(s) 92, 267, 362, 3599, 3680, 3751
AsuC2I CCSGG 4 cut(s) 945, 1589, 2326, 3481
AsuHPI GGTGA 8 cut(s) 49, 56, 617, 733, 2597, 3047, 3704, 3917
AsuNHI GCTAGC 2 cut(s) 1079, 2258
AvaI CYCGRG 1 cut(s) 465
AvaII GGWCC 6 cut(s) 758, 1122, 1243, 1430, 1585, 3436
BaeGI GKGCMC 1 cut(s) 1746
BalI TGGCCA 3 cut(s) 429, 1893, 2195
BamHI GGATCC 1 cut(s) 490
BanI GGYRCC 3 cut(s) 264, 1743, 1940
BanII GRGCYC 5 cut(s) 415, 472, 2066, 2751, 2991
BauI CACGAG 1 cut(s) 2283
BbsI GAAGAC 1 cut(s) 3724
Bbv12I GWGCWC 4 cut(s) 213, 415, 1399, 2751
BceAI ACGGC 2 cut(s) 1529, 3060
BciT130I CCWGG 7 cut(s) 28, 673, 1021, 2583, 3345, 3440, 3651
BciVI GTATCC 1 cut(s) 3010
BcnI CCSGG 4 cut(s) 945, 1589, 2326, 3481
BcoDI GTCTC 5 cut(s) 28, 797, 2042, 3722, 3976
BfmI CTRYAG 4 cut(s) 889, 999, 2088, 3741
BfoI RGCGCY 1 cut(s) 268
BfuAI ACCTGC 2 cut(s) 1900, 3680
BfuI GTATCC 1 cut(s) 3010
BglI GCCNNNNNGGC 2 cut(s) 3503, 3953
BlpI GCTNAGC 1 cut(s) 2990
Bme18I GGWCC 6 cut(s) 758, 1122, 1243, 1430, 1585, 3436
BmeT110I CYCGRG 1 cut(s) 465
BmrI ACTGGG 1 cut(s) 3899
BmsI GCATC 9 cut(s) 245, 1177, 1177, 1183, 1618, 2603, 2609, 2887, 3689
BmtI GCTAGC 2 cut(s) 1083, 2262
BmuI ACTGGG 1 cut(s) 3899
BpiI GAAGAC 1 cut(s) 3724
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BpmI CTGGAG 3 cut(s) 10, 2875, 2969
Bpu10I CCTNAGC 1 cut(s) 2476
Bpu1102I GCTNAGC 1 cut(s) 2990
BpuEI CTTGAG 4 cut(s) 437, 457, 2284, 3495
BpuMI CCSGG 4 cut(s) 945, 1589, 2326, 3481
BsaAI YACGTR 1 cut(s) 2498
BsaHI GRCGYC 1 cut(s) 265
BsaI GGTCTC 1 cut(s) 28
BsaJI CCNNGG 6 cut(s) 741, 1433, 1588, 1935, 2509, 3331
BsaWI WCCGGW 2 cut(s) 1124, 2689
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 2722, 2752, 2848, 2878
Bse3DI GCAATG 2 cut(s) 1014, 2410
BseAI TCCGGA 1 cut(s) 1124
BseBI CCWGG 7 cut(s) 28, 673, 1021, 2583, 3345, 3440, 3651
BseDI CCNNGG 6 cut(s) 741, 1433, 1588, 1935, 2509, 3331
BseGI GGATG 7 cut(s) 236, 296, 1147, 1192, 2385, 2633, 3553
BseMI GCAATG 2 cut(s) 1014, 2410
BseRI GAGGAG 5 cut(s) 85, 2350, 2601, 2676, 2743
BseSI GKGCMC 1 cut(s) 1746
BseYI CCCAGC 1 cut(s) 2764
BsgI GTGCAG 1 cut(s) 3505
Bsh1236I CGCG 2 cut(s) 1038, 3749
Bsh1285I CGRYCG 1 cut(s) 949
BshNI GGYRCC 3 cut(s) 264, 1743, 1940
BsiEI CGRYCG 1 cut(s) 949
BsiHKAI GWGCWC 4 cut(s) 213, 415, 1399, 2751
BsiHKCI CYCGRG 1 cut(s) 465
BsiSI CCGG 7 cut(s) 532, 945, 1125, 1588, 2325, 2690, 3480
BslFI GGGAC 5 cut(s) 26, 569, 1208, 2227, 3449
BsmAI GTCTC 5 cut(s) 28, 797, 2042, 3722, 3976
BsmBI CGTCTC 1 cut(s) 3976
BsmFI GGGAC 5 cut(s) 26, 569, 1208, 2227, 3449
BsmI GAATGC 5 cut(s) 377, 441, 1631, 2368, 2971
Bso31I GGTCTC 1 cut(s) 28
BsoBI CYCGRG 1 cut(s) 465
Bsp1286I GDGCHC 8 cut(s) 213, 415, 472, 1399, 1746, 2066, 2751, 2991
Bsp13I TCCGGA 1 cut(s) 1124
Bsp1407I TGTACA 1 cut(s) 796
Bsp1720I GCTNAGC 1 cut(s) 2990
BspEI TCCGGA 1 cut(s) 1124
BspFNI CGCG 2 cut(s) 1038, 3749
BspHI TCATGA 1 cut(s) 2203
BspMAI CTGCAG 2 cut(s) 893, 1003
BspMI ACCTGC 2 cut(s) 1900, 3680
BspOI GCTAGC 2 cut(s) 1083, 2262
BspQI GCTCTTC 1 cut(s) 403
BspT107I GGYRCC 3 cut(s) 264, 1743, 1940
BspTNI GGTCTC 1 cut(s) 28
BsrBI CCGCTC 2 cut(s) 101, 3807
BsrDI GCAATG 2 cut(s) 1014, 2410
BsrGI TGTACA 1 cut(s) 796
BssECI CCNNGG 6 cut(s) 741, 1433, 1588, 1935, 2509, 3331
BssNI GRCGYC 1 cut(s) 265
BssSI CACGAG 1 cut(s) 2283
BssT1I CCWWGG 4 cut(s) 1433, 1935, 2509, 3331
Bst2BI CACGAG 1 cut(s) 2283
Bst2UI CCWGG 7 cut(s) 28, 673, 1021, 2583, 3345, 3440, 3651
Bst4CI ACNGT 7 cut(s) 52, 325, 773, 962, 2103, 2670, 3387
Bst6I CTCTTC 5 cut(s) 403, 906, 2886, 2901, 3135
BstACI GRCGYC 1 cut(s) 265
BstAPI GCANNNNNTGC 2 cut(s) 2105, 3956
BstAUI TGTACA 1 cut(s) 796
BstBAI YACGTR 1 cut(s) 2498
BstEII GGTNACC 1 cut(s) 3392
BstENI CCTNNNNNAGG 1 cut(s) 3165
BstF5I GGATG 7 cut(s) 236, 296, 1147, 1192, 2385, 2633, 3553
BstFNI CGCG 2 cut(s) 1038, 3749
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 6 cut(s) 92, 267, 362, 3599, 3680, 3751
BstMAI GTCTC 5 cut(s) 28, 797, 2042, 3722, 3976
BstMCI CGRYCG 1 cut(s) 949
BstNI CCWGG 7 cut(s) 28, 673, 1021, 2583, 3345, 3440, 3651
BstNSI RCATGY 2 cut(s) 2653, 3791
BstPI GGTNACC 1 cut(s) 3392
BstSFI CTRYAG 4 cut(s) 889, 999, 2088, 3741
BstSLI GKGCMC 1 cut(s) 1746
BstUI CGCG 2 cut(s) 1038, 3749
BstV2I GAAGAC 1 cut(s) 3724
BstXI CCANNNNNNTGG 2 cut(s) 184, 3899
BsuI GTATCC 1 cut(s) 3010
BtgZI GCGATG 1 cut(s) 112
BtsCI GGATG 7 cut(s) 236, 296, 1147, 1192, 2385, 2633, 3553
BtsI GCAGTG 1 cut(s) 996
BtsIMutI CAGTG 5 cut(s) 996, 1278, 1385, 2283, 3761
BveI ACCTGC 2 cut(s) 1900, 3680
CaiI CAGNNNCTG 2 cut(s) 3706, 3956
CciI TCATGA 1 cut(s) 2203
CfoI GCGC 6 cut(s) 92, 267, 362, 3599, 3680, 3751
CpoI CGGWCCG 1 cut(s) 1122
CseI GACGC 4 cut(s) 242, 3243, 3478, 3755
CsiI ACCWGGT 1 cut(s) 2581
Csp6I GTAC 6 cut(s) 448, 640, 797, 1134, 1941, 3871
CspI CGGWCCG 1 cut(s) 1122
CviQI GTAC 6 cut(s) 448, 640, 797, 1134, 1941, 3871
DinI GGCGCC 1 cut(s) 266
DrdI GACNNNNNNGTC 1 cut(s) 20
DseDI GACNNNNNNGTC 1 cut(s) 20
EaeI YGGCCR 4 cut(s) 427, 1891, 2193, 2575
Eam1104I CTCTTC 5 cut(s) 403, 906, 2886, 2901, 3135
EarI CTCTTC 5 cut(s) 403, 906, 2886, 2901, 3135
EciI GGCGGA 2 cut(s) 160, 2904
Ecl136II GAGCTC 2 cut(s) 413, 2749
Eco130I CCWWGG 4 cut(s) 1433, 1935, 2509, 3331
Eco24I GRGCYC 5 cut(s) 415, 472, 2066, 2751, 2991
Eco31I GGTCTC 1 cut(s) 28
Eco47I GGWCC 6 cut(s) 758, 1122, 1243, 1430, 1585, 3436
Eco53kI GAGCTC 2 cut(s) 413, 2749
Eco57I CTGAAG 2 cut(s) 1346, 2217
Eco88I CYCGRG 1 cut(s) 465
Eco91I GGTNACC 1 cut(s) 3392
EcoICRI GAGCTC 2 cut(s) 413, 2749
EcoNI CCTNNNNNAGG 1 cut(s) 3165
EcoO109I RGGNCCY 2 cut(s) 1430, 3436
EcoO65I GGTNACC 1 cut(s) 3392
EcoRI GAATTC 1 cut(s) 939
EcoRII CCWGG 7 cut(s) 26, 671, 1019, 2581, 3343, 3438, 3649
EcoT14I CCWWGG 4 cut(s) 1433, 1935, 2509, 3331
EcoT22I ATGCAT 3 cut(s) 1192, 1633, 2655
EcoT38I GRGCYC 5 cut(s) 415, 472, 2066, 2751, 2991
EgeI GGCGCC 1 cut(s) 266
EheI GGCGCC 1 cut(s) 266
ErhI CCWWGG 4 cut(s) 1433, 1935, 2509, 3331
Esp3I CGTCTC 1 cut(s) 3976
FalI AAGNNNNNCTT 6 cut(s) 506, 538, 1560, 1592, 3114, 3146
FaqI GGGAC 5 cut(s) 26, 569, 1208, 2227, 3449
FauI CCCGC 4 cut(s) 2073, 2804, 2978, 3800
FauNDI CATATG 2 cut(s) 1179, 1532
FblI GTMKAC 1 cut(s) 3298
FokI GGATG 7 cut(s) 223, 303, 1134, 1199, 2372, 2620, 3560
FriOI GRGCYC 5 cut(s) 415, 472, 2066, 2751, 2991
FspI TGCGCA 1 cut(s) 361
GlaI GCGC 6 cut(s) 91, 266, 361, 3598, 3679, 3750
GsaI CCCAGC 1 cut(s) 2768
GsuI CTGGAG 3 cut(s) 10, 2875, 2969
HaeII RGCGCY 1 cut(s) 268
HapII CCGG 7 cut(s) 532, 945, 1125, 1588, 2325, 2690, 3480
HgaI GACGC 4 cut(s) 242, 3243, 3478, 3755
HhaI GCGC 6 cut(s) 92, 267, 362, 3599, 3680, 3751
Hin1I GRCGYC 1 cut(s) 265
Hin6I GCGC 6 cut(s) 90, 265, 360, 3597, 3678, 3749
HinP1I GCGC 6 cut(s) 90, 265, 360, 3597, 3678, 3749
HincII GTYRAC 2 cut(s) 2673, 3527
HindII GTYRAC 2 cut(s) 2673, 3527
HpaI GTTAAC 1 cut(s) 2673
HpaII CCGG 7 cut(s) 532, 945, 1125, 1588, 2325, 2690, 3480
HphI GGTGA 8 cut(s) 49, 56, 617, 733, 2597, 3047, 3704, 3917
Hpy166II GTNNAC 6 cut(s) 619, 2472, 2673, 3299, 3373, 3527
Hpy8I GTNNAC 6 cut(s) 619, 2472, 2673, 3299, 3373, 3527
Hpy99I CGWCG 6 cut(s) 77, 80, 83, 233, 236, 3572
HpyAV CCTTC 9 cut(s) 745, 760, 1576, 2196, 2282, 2286, 3062, 3892, 3976
HpyCH4III ACNGT 7 cut(s) 52, 325, 773, 962, 2103, 2670, 3387
HpyCH4IV ACGT 6 cut(s) 696, 837, 895, 1645, 2497, 3570
HpySE526I ACGT 6 cut(s) 696, 837, 895, 1645, 2497, 3570
Hsp92I GRCGYC 1 cut(s) 265
HspAI GCGC 6 cut(s) 90, 265, 360, 3597, 3678, 3749
KasI GGCGCC 1 cut(s) 264
Kpn2I TCCGGA 1 cut(s) 1124
KpnI GGTACC 1 cut(s) 1944
KspAI GTTAAC 1 cut(s) 2673
LguI GCTCTTC 1 cut(s) 403
LmnI GCTCC 7 cut(s) 2061, 2754, 2856, 2891, 2950, 3796, 3968
LweI GCATC 9 cut(s) 245, 1177, 1177, 1183, 1618, 2603, 2609, 2887, 3689
MabI ACCWGGT 1 cut(s) 2581
MaeII ACGT 6 cut(s) 696, 837, 895, 1645, 2497, 3570
MbiI CCGCTC 2 cut(s) 101, 3807
MhlI GDGCHC 8 cut(s) 213, 415, 472, 1399, 1746, 2066, 2751, 2991
MlsI TGGCCA 3 cut(s) 429, 1893, 2195
MluNI TGGCCA 3 cut(s) 429, 1893, 2195
Mly113I GGCGCC 1 cut(s) 265
MmeI TCCRAC 7 cut(s) 264, 274, 1441, 2359, 2776, 3263, 3613
Mox20I TGGCCA 3 cut(s) 429, 1893, 2195
Mph1103I ATGCAT 3 cut(s) 1192, 1633, 2655
MroI TCCGGA 1 cut(s) 1124
MroXI GAANNNNTTC 1 cut(s) 884
MscI TGGCCA 3 cut(s) 429, 1893, 2195
MseI TTAA 8 cut(s) 270, 345, 867, 1724, 2672, 2717, 3135, 3843
MslI CAYNNNNRTG 5 cut(s) 182, 880, 2373, 2394, 3786
Msp20I TGGCCA 3 cut(s) 429, 1893, 2195
MspA1I CMGCKG 2 cut(s) 833, 2431
MspI CCGG 7 cut(s) 532, 945, 1125, 1588, 2325, 2690, 3480
Mva1269I GAATGC 5 cut(s) 377, 441, 1631, 2368, 2971
MvaI CCWGG 7 cut(s) 28, 673, 1021, 2583, 3345, 3440, 3651
MvnI CGCG 2 cut(s) 1038, 3749
NarI GGCGCC 1 cut(s) 265
NciI CCSGG 4 cut(s) 945, 1589, 2326, 3481
NdeI CATATG 2 cut(s) 1179, 1532
NheI GCTAGC 2 cut(s) 1079, 2258
NmeAIII GCCGAG 2 cut(s) 766, 3583
NmuCI GTSAC 7 cut(s) 55, 215, 1003, 2864, 3310, 3490, 3692
NsbI TGCGCA 1 cut(s) 361
NsiI ATGCAT 3 cut(s) 1192, 1633, 2655
NspI RCATGY 2 cut(s) 2653, 3791
PagI TCATGA 1 cut(s) 2203
PaqCI CACCTGC 1 cut(s) 3680
PciSI GCTCTTC 1 cut(s) 403
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 237
PctI GAATGC 5 cut(s) 377, 441, 1631, 2368, 2971
PdmI GAANNNNTTC 1 cut(s) 884
PflFI GACNNNGTC 2 cut(s) 245, 506
PflMI CCANNNNNTGG 3 cut(s) 1020, 1852, 2278
PluTI GGCGCC 1 cut(s) 268
Ppu21I YACGTR 1 cut(s) 2498
PpuMI RGGWCCY 2 cut(s) 1430, 3436
PshBI ATTAAT 1 cut(s) 867
Psp124BI GAGCTC 2 cut(s) 415, 2751
Psp5II RGGWCCY 2 cut(s) 1430, 3436
Psp6I CCWGG 7 cut(s) 26, 671, 1019, 2581, 3343, 3438, 3649
PspEI GGTNACC 1 cut(s) 3392
PspFI CCCAGC 1 cut(s) 2764
PspGI CCWGG 7 cut(s) 26, 671, 1019, 2581, 3343, 3438, 3649
PspPPI RGGWCCY 2 cut(s) 1430, 3436
PstI CTGCAG 2 cut(s) 893, 1003
PstNI CAGNNNCTG 2 cut(s) 3706, 3956
PsyI GACNNNGTC 2 cut(s) 245, 506
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 6 cut(s) 449, 641, 798, 1135, 1942, 3872
RsaNI GTAC 6 cut(s) 448, 640, 797, 1134, 1941, 3871
RseI CAYNNNNRTG 5 cut(s) 182, 880, 2373, 2394, 3786
Rsr2I CGGWCCG 1 cut(s) 1122
RsrII CGGWCCG 1 cut(s) 1122
SacI GAGCTC 2 cut(s) 415, 2751
SapI GCTCTTC 1 cut(s) 403
SaqAI TTAA 8 cut(s) 270, 345, 867, 1724, 2672, 2717, 3135, 3843
SduI GDGCHC 8 cut(s) 213, 415, 472, 1399, 1746, 2066, 2751, 2991
SexAI ACCWGGT 1 cut(s) 2581
SfaNI GCATC 9 cut(s) 245, 1177, 1177, 1183, 1618, 2603, 2609, 2887, 3689
SfcI CTRYAG 4 cut(s) 889, 999, 2088, 3741
SfoI GGCGCC 1 cut(s) 266
SinI GGWCC 6 cut(s) 758, 1122, 1243, 1430, 1585, 3436
SmiMI CAYNNNNRTG 5 cut(s) 182, 880, 2373, 2394, 3786
SmlI CTYRAG 4 cut(s) 416, 436, 2299, 3510
SmoI CTYRAG 4 cut(s) 416, 436, 2299, 3510
SspDI GGCGCC 1 cut(s) 264
SspI AATATT 1 cut(s) 2998
SstI GAGCTC 2 cut(s) 415, 2751
StyI CCWWGG 4 cut(s) 1433, 1935, 2509, 3331
TaaI ACNGT 7 cut(s) 52, 325, 773, 962, 2103, 2670, 3387
TaiI ACGT 6 cut(s) 699, 840, 898, 1648, 2500, 3573
TaqI TCGA 8 cut(s) 369, 472, 483, 1443, 1609, 1680, 1749, 3657
TatI WGTACW 2 cut(s) 639, 796
TauI GCSGC 3 cut(s) 176, 2518, 3950
Tru1I TTAA 8 cut(s) 270, 345, 867, 1724, 2672, 2717, 3135, 3843
Tru9I TTAA 8 cut(s) 270, 345, 867, 1724, 2672, 2717, 3135, 3843
TscAI CASTG 5 cut(s) 1003, 1285, 1385, 2283, 3768
TseFI GTSAC 7 cut(s) 55, 215, 1003, 2864, 3310, 3490, 3692
Tsp45I GTSAC 7 cut(s) 55, 215, 1003, 2864, 3310, 3490, 3692
TspGWI ACGGA 5 cut(s) 34, 1268, 2836, 3112, 3257
TspRI CASTG 5 cut(s) 1003, 1285, 1385, 2283, 3768
Tth111I GACNNNGTC 2 cut(s) 245, 506
Van91I CCANNNNNTGG 3 cut(s) 1020, 1852, 2278
VpaK11BI GGWCC 6 cut(s) 758, 1122, 1243, 1430, 1585, 3436
VspI ATTAAT 1 cut(s) 867
XagI CCTNNNNNAGG 1 cut(s) 3165
XapI RAATTY 8 cut(s) 628, 824, 911, 939, 1093, 1991, 2164, 3171
XceI RCATGY 2 cut(s) 2653, 3791
XmiI GTMKAC 1 cut(s) 3298
XmnI GAANNNNTTC 1 cut(s) 884
Zsp2I ATGCAT 3 cut(s) 1192, 1633, 2655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.