pycom04g15040

Regulatory-associated protein of TOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002483
Physical Location & Seq
Reverse (-)
17837718 .. 17847902
10185 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15040.19

Sequence Viewer

Length: 3645 bp
ATGGCATTGGGGGATTTGATGGCCTCCCGGTTTTCGCAATCCTCGGTGGTGGTCTACAGTCAGTTGGACGACTGCGCCTCCAGCCACGACGACGTCGATTTGAGCTCCCAGAGAAGGGAATCAGAGACTGCCAGTAGTAGTTATGGAAATGCCACCGCCACAAGCATGGCCTACTTGCCCCAAACCGTTGTATTGGGTGAACTTCGACACTATGCTTTCGAGGCTTGCGTGCCCACCGGACCATCGGATAGTGGTTTGGTCTCGAAATGGCGGCCTAAAGACCGAATGAAGACGGGATGTGTAGCTCTAGTATTATGTTTAAACATTAGCGTTGATCCGCCTGATGTAATTAAGATATCTCCCTGTGCCAGAATGGAGTGCTGGATAGATCCATTTTCTATGGCTCCACAAAAAGCTCTAGAAACAATTGGTAGAACCTTGAGTGAACAGTACGAAAGGTGGCAACCCAGGGCTCGCTACAAGGTTCAGCTTGATCCTACAGTAGAAGAAGTGAAGAAACTTTGTAATACATGCCGCAAATACGCCAAGTCCGAGAGAGTACTTTTCCACTATAATGGACATGGTGTACCCAAGCCAACTGCTAATGGTGAAATTTGGCTCTTTAATAAGAGTTATACACAGTATATTCCCTTGCCGATTAGTGACCTCGATTCGTGGTTGAAGACACCGTCAATATATGTGTTTGATTGTTCTGCTGCTGGGATGATAGTCAATTCTTTCATTGAGCTTTATGATTTGGGTGGCTCTAGCTCGTCTGGATCTGCAAGAGATTGTATCCTGCTAGCGGCCTGTGAACCACATGAAACTCTGCCACAAAGTGCTGAATTCCCTGCTGATGTGTTTACTTCTTGCCTCACAACTCCCATTAAGATGGCATTAAGATGGTTCTGCACACGATCATTACTTCATGAGTCTCTTGATTATTCGCTAATCGATAAAATACCTGGCCGCCAAAATGACCGGAAAACCCTCCTTGGCGAGTTGAATTGGATTTTTACTGCGGTCACTGATACGATTGCATGGAATGTTCTCCCTCATGATCTTTTCCAGAGACTGTTCAGACAAGACCTGCTAGTTGCCAGCCTTTTTCGTAATTTTTTACTTGCTGAGCGAATTATGAGATCTGCAAATTGCTCTCCGATCTCTTACCCGCAGTTGCCTCCAACCCATCAGCATCATATGTGGGATGCATGGGACATGGCTGCTGAGATATGCCTTTCTCAGCTTCCATCATTGGTTGAGGATCCTAATGCGGAGTTCCAGCCAAGTCCATTTTTCACTGAGCAGTTGACTGCTTTTGAGGTATGGCTTGACCATGGGTCTGAACTCAAGAAACCACCAGAGCAGTTGCCTATTGTTCTTCAGGTGTTACTAAGTCAATGCCACCGATCTCGAGCTCTGGTTCTTCTTGGAAGATTCCTTGATATGGGATCCTGGGCTGTAGATCTGGCCTTGTCTGTTGGGATATTTCCATACGTTCTGAAGCTGTTACAAACAATGACACCTGAACTACGACAAATTCTTGTATTTATTTGGACAAAAATTCTTGCTCTTGATAAGTCATGTCAGGTTGATCTAGTGAAGGATGGGGGTCATACATATTTCATCAGGTTTCTTGATAGTATGGAAGCGTATCCAGAACAGCGTGCAATGGCTGCTTTTGTTTTAGCTGTAATTGTCGATGGGCATAGACGCGGGCAGGAAGCTTGTATTGAAGCAGGTTTGATACATGTTTGCTTGAAGCACCTTCAGGGTCCAACTCTAAATGATACACAGACAGAACCGCTATTTCTTCAGTGGCTTTGTCTCTGTCTTGGGAAGCTGTGGGAGGATTTCACAGAGGCCCAAATATTTGGTTTGCAGGCAGATGCCCCTGCAATATGTGCTTCTCTACTTTCTGAGCCCCAACCAGAGGTTAGGGCTTCTGCTGTTTTCGCACTGGGTACCCTGCTTGATGTGGGTTCAGGCAATGGCGTTGGTGGAGAAGAAGAATATGATGATGATGAAAAGATTAGTGCTGAAATTAGTATTGTTAGAAGTCTACTAAGTGTTGCTTCAGATGGGAGCCCTCTGGTCAGGGCCGAAGTTGCTGTTGCACTGGGACGCTTTGCCTTTGGCCATAACAAGCACCTCAAGTCAATTGCTGCTGCATATTGGAAACCACAATCTAATTCTGTTTTGAATTCCTTGCCCTCTTTGGCTCATATGAAAGGTAACGTTGTTTCATCTCAAATTGGTCCACTTTTGAGAGGGACCAATGACAACTCATTGGGTCGAGATGGAAGAGTCTCCACCAGCAGCCCTCTAGCGAGTTCTGGAATTATGCATGGATCACCATTGTCTGACGATTCATCTCATCATTCTGATTCTGGAATATTAAATGATGGTGTGAGCAATGGTGTTGTCAACCACTCAACACCTGAACCCCTGGACAATGCAATTTACTCTCTGTGTGTATTGGCTATGTGTACTCTAGCCAAGGATCCATCTCCACGCATTGCAAGCCTTGGTAGGCGGGTTTTGGCCATTATAGGGATTGAACAAGTGGTGGCCAAACCCATGAAGTCCACTGGTAACAGTGTTCGACCTGGTGAATCCATTACAGCAACTCCAATTCCCAGTTTTTCTGGATTAGCTCGATCTTCTTCATGGTTTGATATGAATGGAGGTCATATGCCATTCCGAACTCCTCCTGTCAGCCCTCCTCGGCCCAATTACTTAGCTGGAATGCGGAGAGTATACTCTTTAGAGCTCAGGCCTCATATAATGAGTCCAGATTCTGGATTAGCGGATCCACTTTTAGGTTCTGCAGGAACTTCTGGAGCCTCGGAACGCACTGTGAGCAAGCTTAATAATCAAATTGCTAGCTGGGACACCAAATTTGAGACGGGTACAAAAACAATCTTGCTGAATCCATTTTCTCCTATTGTGGTTGCGGCAGACGAGAAAGAGCGAATCAGGGTATGGAACTACCAAGAGCAGAAGGAGGCTACCCTTCTCAACAGCTTCGATAACCATGATTTTCCTGACAAAGGAATCGCCAAGCTCTGCCTTGTGAATGAGCTTGATGACAGCTTGCTTCTTGCTGCTTCAGATTGTAGCATCTCAGCACTGTCTGCTTCTCAGGTTCACGTTGGTCACTTTGCAGCTGGTTTTGTGGATGGTTCTGTCAGGCTTTATGATGTGCGAACTCCTGAAATGCTTGTCTGTTCAACCCGGCCACACACTCAGAAGGTGGAAAGAGTTGTGGGGATTGGCTTTCAACCTGGGCTTGATCCTGGCAAGATTGTAAGTGCGTCTCAAGCTGGTGACATTCAGTTCCTTGATATCAGAAACGACAGGGATGCCTACCTTACGATTGAAGCTCACCGCGGTTCACTGACAGCTTTAGCTGTTCACAGGCATGCCCCAATCATTGCCAGTGGCTCGGCCAAACAGCTCATAAAAGTCTTCAGCTTGGAGGGCGAACAATTGGGCACAATAAGATACTACCCTTCCTTTATGGCCCAAAAGATTGGTCCTGTTAGTAGCCTAGCCTTCCATCCCTATGAAGTGCTGCTTGCAGCTGGTGCCGCAGATGCTTGCGCATCCATTTATGCTGACGACAACTCTCATGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0001101 GO:0001558 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008361 GO:0009267 GO:0009605 GO:0009719 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009893 GO:0009894 GO:0009987 GO:0009991 GO:0010033 GO:0010073 GO:0010074 GO:0010154 GO:0010243 GO:0010492 GO:0010506 GO:0010562 GO:0010604 GO:0016043 GO:0019220 GO:0019222 GO:0019827 GO:0022414 GO:0023052 GO:0030307 GO:0030674 GO:0031323 GO:0031325 GO:0031329 GO:0031399 GO:0031401 GO:0031461 GO:0031667 GO:0031668 GO:0031669 GO:0031929 GO:0031931 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032535 GO:0032991 GO:0033554 GO:0033674 GO:0035556 GO:0038201 GO:0040008 GO:0042221 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043200 GO:0043549 GO:0044093 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045927 GO:0045937 GO:0048316 GO:0048507 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0060090 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071840 GO:0071900 GO:0071902 GO:0080008 GO:0080090 GO:0090066 GO:0098727 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04136 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04714 ko04910 ko05206 map04136 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04714 map04910 map05206
Pfam Domains
Protein Families
HEAT Raptor_N WD40

Protein Analysis

1215

Amino Acids

132.95

Weight (kDa)

5.73

Isoelectric Point (pI)

45.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 96
Acc16I TGCGCA 1 cut(s) 3607
Acc36I ACCTGC 2 cut(s) 1098, 1730
Acc65I GGTACC 1 cut(s) 1964
AccB1I GGYRCC 2 cut(s) 1964, 3590
AccB7I CCANNNNNTGG 1 cut(s) 2801
AccI GTMKAC 3 cut(s) 54, 2062, 2760
AccII CGCG 2 cut(s) 1716, 3393
AclI AACGTT 1 cut(s) 2238
AcoI YGGCCR 6 cut(s) 967, 2137, 2544, 2571, 3239, 3450
AcsI RAATTY 6 cut(s) 612, 845, 1539, 1563, 2203, 2900
AcuI CTGAAG 7 cut(s) 1367, 1523, 1754, 1799, 2061, 3096, 3457
AcyI GRCGYC 1 cut(s) 93
AdeI CACNNNGTG 1 cut(s) 839
AfaI GTAC 6 cut(s) 452, 561, 588, 1966, 2491, 2914
AfiI CCNNNNNNNGG 9 cut(s) 114, 115, 805, 1447, 1933, 2553, 2801, 2822, 3053
AflIII ACRYGT 1 cut(s) 1750
AgsI TTSAA 9 cut(s) 682, 1006, 1736, 1762, 2203, 2561, 3234, 3284, 3383
AhdI GACNNNNNGTC 1 cut(s) 1339
AjnI CCWGG 7 cut(s) 467, 964, 1454, 2448, 2608, 3286, 3298
Alw21I GWGCWC 3 cut(s) 107, 1420, 2775
Alw26I GTCTC 8 cut(s) 119, 265, 939, 1066, 1832, 2314, 2900, 3324
AlwNI CAGNNNCTG 3 cut(s) 128, 1075, 2801
Ama87I CYCGRG 1 cut(s) 1413
ApoI RAATTY 6 cut(s) 612, 845, 1539, 1563, 2203, 2900
ArsI GACNNNNNNTTYG 4 cut(s) 2885, 2917, 3523, 3555
Asp718I GGTACC 1 cut(s) 1964
AspLEI GCGC 2 cut(s) 77, 3608
AspS9I GGNCC 9 cut(s) 239, 1775, 1864, 2100, 2258, 2274, 2730, 3526, 3539
AsuC2I CCSGG 2 cut(s) 28, 3238
AsuHPI GGTGA 6 cut(s) 209, 620, 2346, 2624, 3341, 3380
AsuNHI GCTAGC 2 cut(s) 802, 2885
AvaI CYCGRG 1 cut(s) 1413
AvaII GGWCC 5 cut(s) 239, 1775, 2258, 2274, 3539
BaeGI GKGCMC 2 cut(s) 234, 3500
BalI TGGCCA 3 cut(s) 2139, 2546, 2573
BamHI GGATCC 4 cut(s) 1264, 1451, 2503, 2812
BanI GGYRCC 2 cut(s) 1964, 3590
BanII GRGCYC 6 cut(s) 107, 475, 1420, 1926, 2090, 2775
BbsI GAAGAC 3 cut(s) 296, 689, 3463
Bbv12I GWGCWC 3 cut(s) 107, 1420, 2775
BcgI CGANNNNNNTGC 2 cut(s) 3347, 3381
BciT130I CCWGG 7 cut(s) 469, 966, 1456, 2450, 2610, 3288, 3300
BciVI GTATCC 2 cut(s) 806, 1665
BcnI CCSGG 2 cut(s) 28, 3238
BcoDI GTCTC 8 cut(s) 119, 265, 939, 1066, 1832, 2314, 2900, 3324
BfmI CTRYAG 4 cut(s) 55, 498, 1461, 2829
BfuAI ACCTGC 2 cut(s) 1098, 1730
BfuI GTATCC 2 cut(s) 806, 1665
BglII AGATCT 2 cut(s) 1142, 1465
BlpI GCTNAGC 1 cut(s) 1128
BmcAI AGTACT 1 cut(s) 561
Bme1390I CCNGG 9 cut(s) 28, 469, 966, 1456, 2450, 2610, 3238, 3288, 3300
Bme18I GGWCC 5 cut(s) 239, 1775, 2258, 2274, 3539
BmeRI GACNNNNNGTC 1 cut(s) 1339
BmeT110I CYCGRG 1 cut(s) 1413
BmgT120I GGNCC 9 cut(s) 239, 1775, 1864, 2100, 2258, 2274, 2730, 3526, 3539
BmrFI CCNGG 9 cut(s) 28, 469, 966, 1456, 2450, 2610, 3238, 3288, 3300
BmrI ACTGGG 3 cut(s) 1970, 2129, 2634
BmsI GCATC 7 cut(s) 1198, 1204, 1879, 3132, 3355, 3589, 3617
BmtI GCTAGC 2 cut(s) 806, 2889
BmuI ACTGGG 3 cut(s) 1970, 2129, 2634
BpiI GAAGAC 3 cut(s) 296, 689, 3463
BpmI CTGGAG 2 cut(s) 64, 2862
Bpu10I CCTNAGC 1 cut(s) 2774
Bpu1102I GCTNAGC 1 cut(s) 1128
BpuEI CTTGAG 4 cut(s) 460, 1334, 2138, 3306
BpuMI CCSGG 2 cut(s) 28, 3238
Bsa29I ATCGAT 1 cut(s) 954
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 265
BsaWI WCCGGW 2 cut(s) 236, 981
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 2614, 2644
Bsc4I CCNNNNNNNGG 9 cut(s) 114, 115, 805, 1447, 1933, 2553, 2801, 2822, 3053
Bse1I ACTGG 6 cut(s) 132, 1965, 2124, 2596, 2640, 3441
Bse3DI GCAATG 5 cut(s) 1677, 1996, 2422, 2517, 3435
BseBI CCWGG 7 cut(s) 469, 966, 1456, 2450, 2610, 3288, 3300
BseCI ATCGAT 1 cut(s) 954
BseGI GGATG 8 cut(s) 302, 729, 1213, 1612, 3187, 3370, 3562, 3608
BseLI CCNNNNNNNGG 9 cut(s) 114, 115, 805, 1447, 1933, 2553, 2801, 2822, 3053
BseMI GCAATG 5 cut(s) 1677, 1996, 2422, 2517, 3435
BseMII CTCAG 9 cut(s) 1119, 1218, 1256, 1293, 1911, 2788, 3141, 3158, 3263
BseNI ACTGG 6 cut(s) 132, 1965, 2124, 2596, 2640, 3441
BseRI GAGGAG 2 cut(s) 2700, 2715
BseSI GKGCMC 2 cut(s) 234, 3500
BseYI CCCAGC 2 cut(s) 719, 2889
BsgI GTGCAG 1 cut(s) 895
Bsh1236I CGCG 2 cut(s) 1716, 3393
BshNI GGYRCC 2 cut(s) 1964, 3590
BshVI ATCGAT 1 cut(s) 954
BsiHKAI GWGCWC 3 cut(s) 107, 1420, 2775
BsiHKCI CYCGRG 1 cut(s) 1413
BsiSI CCGG 4 cut(s) 28, 237, 982, 3238
BslFI GGGAC 4 cut(s) 1229, 2136, 2287, 2906
BslI CCNNNNNNNGG 9 cut(s) 114, 115, 805, 1447, 1933, 2553, 2801, 2822, 3053
BsmAI GTCTC 8 cut(s) 119, 265, 939, 1066, 1832, 2314, 2900, 3324
BsmBI CGTCTC 2 cut(s) 2900, 3324
BsmFI GGGAC 4 cut(s) 1229, 2136, 2287, 2906
BsmI GAATGC 1 cut(s) 2754
Bso31I GGTCTC 1 cut(s) 265
BsoBI CYCGRG 1 cut(s) 1413
Bsp1286I GDGCHC 8 cut(s) 107, 234, 475, 1420, 1926, 2090, 2775, 3500
Bsp1720I GCTNAGC 1 cut(s) 1128
Bsp19I CCATGG 1 cut(s) 1336
BspCNI CTCAG 9 cut(s) 1120, 1219, 1255, 1294, 1912, 2787, 3140, 3157, 3262
BspDI ATCGAT 1 cut(s) 954
BspFNI CGCG 2 cut(s) 1716, 3393
BspHI TCATGA 2 cut(s) 928, 1057
BspMAI CTGCAG 1 cut(s) 2833
BspMI ACCTGC 2 cut(s) 1098, 1730
BspOI GCTAGC 2 cut(s) 806, 2889
BspT107I GGYRCC 2 cut(s) 1964, 3590
BspTNI GGTCTC 1 cut(s) 265
BsrDI GCAATG 5 cut(s) 1677, 1996, 2422, 2517, 3435
BsrI ACTGG 6 cut(s) 132, 1965, 2124, 2596, 2640, 3441
BssNAI GTATAC 1 cut(s) 2761
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 4 cut(s) 994, 1336, 2499, 2527
Bst1107I GTATAC 1 cut(s) 2761
Bst2UI CCWGG 7 cut(s) 469, 966, 1456, 2450, 2610, 3288, 3300
Bst6I CTCTTC 1 cut(s) 2299
BstACI GRCGYC 1 cut(s) 93
BstAPI GCANNNNNTGC 4 cut(s) 1676, 1904, 3137, 3590
BstDSI CCRYGG 2 cut(s) 1336, 3391
BstENI CCTNNNNNAGG 1 cut(s) 3051
BstF5I GGATG 8 cut(s) 302, 729, 1213, 1612, 3187, 3370, 3562, 3608
BstFNI CGCG 2 cut(s) 1716, 3393
BstHHI GCGC 2 cut(s) 77, 3608
BstMAI GTCTC 8 cut(s) 119, 265, 939, 1066, 1832, 2314, 2900, 3324
BstNI CCWGG 7 cut(s) 469, 966, 1456, 2450, 2610, 3288, 3300
BstNSI RCATGY 3 cut(s) 534, 1754, 3428
BstSCI CCNGG 9 cut(s) 26, 467, 964, 1454, 2448, 2608, 3236, 3286, 3298
BstSFI CTRYAG 4 cut(s) 55, 498, 1461, 2829
BstSLI GKGCMC 2 cut(s) 234, 3500
BstUI CGCG 2 cut(s) 1716, 3393
BstV2I GAAGAC 3 cut(s) 296, 689, 3463
BstX2I RGATCY 8 cut(s) 388, 779, 1142, 1264, 1451, 1465, 2503, 2812
BstXI CCANNNNNNTGG 5 cut(s) 166, 575, 892, 1874, 3536
BstYI RGATCY 8 cut(s) 388, 779, 1142, 1264, 1451, 1465, 2503, 2812
BstZ17I GTATAC 1 cut(s) 2761
Bsu15I ATCGAT 1 cut(s) 954
BsuI GTATCC 2 cut(s) 806, 1665
BsuTUI ATCGAT 1 cut(s) 954
BtgI CCRYGG 2 cut(s) 1336, 3391
BtsCI GGATG 8 cut(s) 302, 729, 1213, 1612, 3187, 3370, 3562, 3608
BveI ACCTGC 2 cut(s) 1098, 1730
CaiI CAGNNNCTG 3 cut(s) 128, 1075, 2801
CciI TCATGA 2 cut(s) 928, 1057
CfoI GCGC 2 cut(s) 77, 3608
Cfr13I GGNCC 9 cut(s) 239, 1775, 1864, 2100, 2258, 2274, 2730, 3526, 3539
Cfr42I CCGCGG 1 cut(s) 3394
ClaI ATCGAT 1 cut(s) 954
CseI GACGC 3 cut(s) 1722, 2133, 3306
CsiI ACCWGGT 1 cut(s) 2608
Csp6I GTAC 6 cut(s) 451, 560, 587, 1965, 2490, 2913
CviQI GTAC 6 cut(s) 451, 560, 587, 1965, 2490, 2913
DraI TTTAAA 1 cut(s) 321
DraIII CACNNNGTG 1 cut(s) 839
DriI GACNNNNNGTC 1 cut(s) 1339
EaeI YGGCCR 6 cut(s) 967, 2137, 2544, 2571, 3239, 3450
Eam1104I CTCTTC 1 cut(s) 2299
Eam1105I GACNNNNNGTC 1 cut(s) 1339
EarI CTCTTC 1 cut(s) 2299
EciI GGCGGA 1 cut(s) 327
Ecl136II GAGCTC 3 cut(s) 105, 1418, 2773
Eco130I CCWWGG 4 cut(s) 994, 1336, 2499, 2527
Eco147I AGGCCT 1 cut(s) 2779
Eco24I GRGCYC 6 cut(s) 107, 475, 1420, 1926, 2090, 2775
Eco31I GGTCTC 1 cut(s) 265
Eco32I GATATC 2 cut(s) 357, 3349
Eco47I GGWCC 5 cut(s) 239, 1775, 2258, 2274, 3539
Eco53kI GAGCTC 3 cut(s) 105, 1418, 2773
Eco57I CTGAAG 7 cut(s) 1367, 1523, 1754, 1799, 2061, 3096, 3457
Eco88I CYCGRG 1 cut(s) 1413
EcoICRI GAGCTC 3 cut(s) 105, 1418, 2773
EcoNI CCTNNNNNAGG 1 cut(s) 3051
EcoRI GAATTC 2 cut(s) 845, 2203
EcoRII CCWGG 7 cut(s) 467, 964, 1454, 2448, 2608, 3286, 3298
EcoRV GATATC 2 cut(s) 357, 3349
EcoT14I CCWWGG 4 cut(s) 994, 1336, 2499, 2527
EcoT22I ATGCAT 2 cut(s) 1213, 2349
EcoT38I GRGCYC 6 cut(s) 107, 475, 1420, 1926, 2090, 2775
ErhI CCWWGG 4 cut(s) 994, 1336, 2499, 2527
Esp3I CGTCTC 2 cut(s) 2900, 3324
FalI AAGNNNNNCTT 4 cut(s) 2059, 2091, 3564, 3596
FaqI GGGAC 4 cut(s) 1229, 2136, 2287, 2906
FauI CCCGC 3 cut(s) 1179, 1709, 2529
FauNDI CATATG 3 cut(s) 1200, 2226, 2694
FblI GTMKAC 3 cut(s) 54, 2062, 2760
FokI GGATG 8 cut(s) 309, 736, 1220, 1619, 3194, 3377, 3549, 3595
FriOI GRGCYC 6 cut(s) 107, 475, 1420, 1926, 2090, 2775
FspI TGCGCA 1 cut(s) 3607
GlaI GCGC 2 cut(s) 76, 3607
GsaI CCCAGC 2 cut(s) 723, 2893
GsuI CTGGAG 2 cut(s) 64, 2862
HapII CCGG 4 cut(s) 28, 237, 982, 3238
HgaI GACGC 3 cut(s) 1722, 2133, 3306
HhaI GCGC 2 cut(s) 77, 3608
Hin1I GRCGYC 1 cut(s) 93
Hin6I GCGC 2 cut(s) 75, 3606
HinP1I GCGC 2 cut(s) 75, 3606
HincII GTYRAC 2 cut(s) 1311, 2428
HindII GTYRAC 2 cut(s) 1311, 2428
HindIII AAGCTT 2 cut(s) 1725, 2867
HpaII CCGG 4 cut(s) 28, 237, 982, 3238
HphI GGTGA 6 cut(s) 209, 620, 2346, 2624, 3341, 3380
Hpy99I CGWCG 3 cut(s) 92, 95, 98
HpyAV CCTTC 8 cut(s) 108, 1597, 1778, 2998, 3026, 3247, 3525, 3568
HpyCH4IV ACGT 4 cut(s) 93, 1497, 2238, 3153
HpySE526I ACGT 4 cut(s) 93, 1497, 2238, 3153
Hsp92I GRCGYC 1 cut(s) 93
HspAI GCGC 2 cut(s) 75, 3606
KpnI GGTACC 1 cut(s) 1968
KspI CCGCGG 1 cut(s) 3394
LmnI GCTCC 4 cut(s) 110, 409, 2085, 2843
LweI GCATC 7 cut(s) 1198, 1204, 1879, 3132, 3355, 3589, 3617
MabI ACCWGGT 1 cut(s) 2608
MaeII ACGT 4 cut(s) 93, 1497, 2238, 3153
MaeIII GTNAC 8 cut(s) 662, 1024, 1389, 1509, 2234, 2594, 3158, 3329
MfeI CAATTG 3 cut(s) 426, 2160, 3491
MflI RGATCY 8 cut(s) 388, 779, 1142, 1264, 1451, 1465, 2503, 2812
MhlI GDGCHC 8 cut(s) 107, 234, 475, 1420, 1926, 2090, 2775, 3500
MlsI TGGCCA 3 cut(s) 2139, 2546, 2573
MluNI TGGCCA 3 cut(s) 2139, 2546, 2573
MlyI GAGTC 3 cut(s) 941, 2316, 2800
MmeI TCCRAC 3 cut(s) 45, 1208, 1802
Mox20I TGGCCA 3 cut(s) 2139, 2546, 2573
Mph1103I ATGCAT 2 cut(s) 1213, 2349
MscI TGGCCA 3 cut(s) 2139, 2546, 2573
MseI TTAA 7 cut(s) 320, 351, 624, 888, 899, 2399, 2871
MslI CAYNNNNRTG 6 cut(s) 164, 573, 890, 901, 3423, 3567
Msp20I TGGCCA 3 cut(s) 2139, 2546, 2573
MspA1I CMGCKG 3 cut(s) 3170, 3393, 3587
MspI CCGG 4 cut(s) 28, 237, 982, 3238
MspR9I CCNGG 9 cut(s) 28, 469, 966, 1456, 2450, 2610, 3238, 3288, 3300
MssI GTTTAAAC 1 cut(s) 321
MunI CAATTG 3 cut(s) 426, 2160, 3491
Mva1269I GAATGC 1 cut(s) 2754
MvaI CCWGG 7 cut(s) 469, 966, 1456, 2450, 2610, 3288, 3300
MvnI CGCG 2 cut(s) 1716, 3393
NciI CCSGG 2 cut(s) 28, 3238
NcoI CCATGG 1 cut(s) 1336
NdeI CATATG 3 cut(s) 1200, 2226, 2694
NheI GCTAGC 2 cut(s) 802, 2885
NmeAIII GCCGAG 2 cut(s) 2707, 3428
NmuCI GTSAC 4 cut(s) 662, 1024, 3158, 3329
NsbI TGCGCA 1 cut(s) 3607
NsiI ATGCAT 2 cut(s) 1213, 2349
NspI RCATGY 3 cut(s) 534, 1754, 3428
PaeI GCATGC 1 cut(s) 3428
PaeR7I CTCGAG 1 cut(s) 1413
PagI TCATGA 2 cut(s) 928, 1057
PasI CCCWGGG 1 cut(s) 468
PceI AGGCCT 1 cut(s) 2779
PciI ACATGT 1 cut(s) 1750
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 225, 549
PctI GAATGC 1 cut(s) 2754
PflFI GACNNNGTC 2 cut(s) 92, 688
PflMI CCANNNNNTGG 1 cut(s) 2801
PleI GAGTC 3 cut(s) 940, 2315, 2799
PmeI GTTTAAAC 1 cut(s) 321
PpsI GAGTC 3 cut(s) 940, 2315, 2799
PscI ACATGT 1 cut(s) 1750
Psp124BI GAGCTC 3 cut(s) 107, 1420, 2775
Psp1406I AACGTT 1 cut(s) 2238
Psp6I CCWGG 7 cut(s) 467, 964, 1454, 2448, 2608, 3286, 3298
PspFI CCCAGC 2 cut(s) 719, 2889
PspGI CCWGG 7 cut(s) 467, 964, 1454, 2448, 2608, 3286, 3298
PspPI GGNCC 9 cut(s) 239, 1775, 1864, 2100, 2258, 2274, 2730, 3526, 3539
PstI CTGCAG 1 cut(s) 2833
PstNI CAGNNNCTG 3 cut(s) 128, 1075, 2801
PsuI RGATCY 8 cut(s) 388, 779, 1142, 1264, 1451, 1465, 2503, 2812
PsyI GACNNNGTC 2 cut(s) 92, 688
PvuII CAGCTG 2 cut(s) 3170, 3587
RsaI GTAC 6 cut(s) 452, 561, 588, 1966, 2491, 2914
RsaNI GTAC 6 cut(s) 451, 560, 587, 1965, 2490, 2913
RseI CAYNNNNRTG 6 cut(s) 164, 573, 890, 901, 3423, 3567
SacI GAGCTC 3 cut(s) 107, 1420, 2775
SacII CCGCGG 1 cut(s) 3394
SaqAI TTAA 7 cut(s) 320, 351, 624, 888, 899, 2399, 2871
Sau96I GGNCC 9 cut(s) 239, 1775, 1864, 2100, 2258, 2274, 2730, 3526, 3539
ScaI AGTACT 1 cut(s) 561
SchI GAGTC 3 cut(s) 941, 2316, 2800
ScrFI CCNGG 9 cut(s) 28, 469, 966, 1456, 2450, 2610, 3238, 3288, 3300
SduI GDGCHC 8 cut(s) 107, 234, 475, 1420, 1926, 2090, 2775, 3500
SexAI ACCWGGT 1 cut(s) 2608
SfaNI GCATC 7 cut(s) 1198, 1204, 1879, 3132, 3355, 3589, 3617
SfcI CTRYAG 4 cut(s) 55, 498, 1461, 2829
Sfr274I CTCGAG 1 cut(s) 1413
Sfr303I CCGCGG 1 cut(s) 3394
SgrBI CCGCGG 1 cut(s) 3394
SinI GGWCC 5 cut(s) 239, 1775, 2258, 2274, 3539
SlaI CTCGAG 1 cut(s) 1413
SmiMI CAYNNNNRTG 6 cut(s) 164, 573, 890, 901, 3423, 3567
SmlI CTYRAG 5 cut(s) 439, 1349, 1413, 2153, 3321
SmoI CTYRAG 5 cut(s) 439, 1349, 1413, 2153, 3321
SphI GCATGC 1 cut(s) 3428
SseBI AGGCCT 1 cut(s) 2779
SspI AATATT 2 cut(s) 1872, 2397
SstI GAGCTC 3 cut(s) 107, 1420, 2775
StuI AGGCCT 1 cut(s) 2779
StyD4I CCNGG 9 cut(s) 26, 467, 964, 1454, 2448, 2608, 3236, 3286, 3298
StyI CCWWGG 4 cut(s) 994, 1336, 2499, 2527
TaiI ACGT 4 cut(s) 96, 1500, 2241, 3156
TaqII GACCGA 1 cut(s) 297
TatI WGTACW 2 cut(s) 559, 2489
TauI GCSGC 6 cut(s) 274, 537, 809, 972, 2960, 3596
Tru1I TTAA 7 cut(s) 320, 351, 624, 888, 899, 2399, 2871
Tru9I TTAA 7 cut(s) 320, 351, 624, 888, 899, 2399, 2871
TseFI GTSAC 4 cut(s) 662, 1024, 3158, 3329
Tsp45I GTSAC 4 cut(s) 662, 1024, 3158, 3329
Tth111I GACNNNGTC 2 cut(s) 92, 688
Van91I CCANNNNNTGG 1 cut(s) 2801
VpaK11BI GGWCC 5 cut(s) 239, 1775, 2258, 2274, 3539
XagI CCTNNNNNAGG 1 cut(s) 3051
XapI RAATTY 6 cut(s) 612, 845, 1539, 1563, 2203, 2900
XbaI TCTAGA 1 cut(s) 418
XceI RCATGY 3 cut(s) 534, 1754, 3428
XhoI CTCGAG 1 cut(s) 1413
XmiI GTMKAC 3 cut(s) 54, 2062, 2760
ZraI GACGTC 1 cut(s) 94
ZrmI AGTACT 1 cut(s) 561
Zsp2I ATGCAT 2 cut(s) 1213, 2349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.