AT3G19540

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002346
Physical Location & Seq
Forward (+)
6780563 .. 6783166
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G19540.1

Sequence Viewer

Length: 1458 bp
ATGGAGAAAAAACAAGGCTTTTTCTCTGCTCTCAGACACGAAGTTGTGAGAGGTCTTTCGCCGTCACGATCCAGAGCAAGGTCTCGTTCCGTTAGTCCCGCTAGGTCTAGTTCACCCATGTCAGCTCTCACTTGGGGAAGAAAGAACTTAACTGGAGGAGGCGGTGGTGGTGGTGGCGGAGGTTACTATTTAGCGCAACCAGAGCAGTTAATTGGGAGATCCGGAAGTTTGAGACCTGTAATGGAAGGTCCTGATCCAGATGAAGGAGGAGGAGGTGGTAATATAGGAGAATCTAAACGACTCGGGTCGGGTTTAGGTCACTGGGTTAAAGGGCAGCTGTCTCGTGCTCCGTCTGTTGCTGCGACGGCGGCTTATCGGAGAAATGATCTGAGACTTCTTCTTGGTGTAATGGGAGCTCCTCTTGCTCCTATTCATGTCTCTTCCTCTGATCCTTTGCCTCATCTTAGTATCAAAAACACTCCAATCGAAACATCATCTGCTCAGTATATTCTTCAACAGTACACTGCAGCTTCTGGTGGTCAGAAGCTACAAAACTCCATTAAAAACGCTTATGCGATGGGGAAGCTCAAGATGATTACTTCAGAGCTTGAAACTGCTACAAGAACCGTTAGGAATCGAAACCCATCTAAGGCTGAGACTGGAGGTTTTGTTCTTTGGCAGATGAATCCAGACATGTGGTATGTTGAGCTTGCTGTTGGGGGTAGTAAGGTTCGTGCTGGCTGCAATGGCAAGCTTGTATGGAGACACACTCCTTGGCTCGGATCTCATACTGCTAAAGGACCTGTCAGACCTCTCCGCCGTGGACTTCAGGGACTAGATCCGAGAACAACTGCTGCAATGTTTGCGGAAGCGAAGTGTATAGGAGAGAAGAAGGTGAATGGTGAAGATTGCTTCATTTTGAAGTTATGCACTGACCCTGAAACACTAAAAGCGAGGAGCGAAGGACCGGCTGAGATCATTAGGCATGTCCTCTTTGGCTACTTCAGTCAGAAAACAGGGCTCTTGGTTCACATTGAGGATTCACATTTGACCCGGATTCAATCTAACGGTGGAGAGACTGTTTTCTGGGAGACCACATACAACTCGTCCCTAGACGATTACCGTCAGGTCGAAGGAATCATGATTGCTCACTCGGGACATTCAGTTGTGACACTATTCAGATTTGGGGAAGTGGCGACGAGCCATACGAGGACAAAGATGGAAGAAAGCTGGACGATTGAGGAAGTTGCGTTTAACGTTCCTGGTCTGTCTCTCGATTGCTTTATACCACCAGCTGATCTTAAGACCGGTTCTTTAACCGAATCCTGCGAGTACCCACAAGAGGAAAGAGGCAAGAACAACACACTCGCATTGTCTGCAGCTCACAGGGCCAAAGTTGCAGCCTTGGAGAACGGGAGCTTGGAAGATCACCGCCCGGTTTGGCATACTGATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

485

Amino Acids

52.56

Weight (kDa)

9.24

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 191 - 431 1.2e-118 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 221
AciI CCGC 7 cut(s) 99, 162, 177, 368, 817, 866, 1432
AclI AACGTT 1 cut(s) 1257
AclWI GGATC 6 cut(s) 63, 213, 248, 443, 790, 833
AcuI CTGAAG 3 cut(s) 585, 812, 988
AfaI GTAC 2 cut(s) 521, 1334
AfiI CCNNNNNNNGG 5 cut(s) 78, 263, 649, 779, 1342
AflII CTTAAG 1 cut(s) 1301
AflIII ACRYGT 1 cut(s) 693
AgeI ACCGGT 1 cut(s) 1307
AgsI TTSAA 4 cut(s) 515, 611, 922, 1061
AjnI CCWGG 1 cut(s) 1261
AjuI GAANNNNNNNTTGG 2 cut(s) 1403, 1435
AloI GAACNNNNNNTCC 2 cut(s) 654, 686
Alw21I GWGCWC 2 cut(s) 349, 418
AlwI GGATC 6 cut(s) 63, 213, 248, 443, 790, 833
AlwNI CAGNNNCTG 1 cut(s) 533
Ama87I CYCGRG 2 cut(s) 302, 1153
Aor13HI TCCGGA 1 cut(s) 221
AoxI GGCC 1 cut(s) 1389
ApeKI GCWGC 7 cut(s) 334, 359, 527, 741, 854, 1379, 1400
AsiGI ACCGGT 1 cut(s) 1307
AspLEI GCGC 1 cut(s) 196
AspS9I GGNCC 4 cut(s) 248, 800, 965, 1389
AsuC2I CCSGG 2 cut(s) 1054, 1436
AsuHPI GGTGA 4 cut(s) 105, 907, 914, 1421
AvaI CYCGRG 2 cut(s) 302, 1153
AvaII GGWCC 3 cut(s) 248, 800, 965
BanII GRGCYC 2 cut(s) 418, 1023
BauI CACGAG 1 cut(s) 342
Bbv12I GWGCWC 2 cut(s) 349, 418
BbvI GCAGC 7 cut(s) 346, 346, 539, 728, 841, 1391, 1412
BccI CCATC 3 cut(s) 571, 652, 1213
BceAI ACGGC 3 cut(s) 46, 381, 804
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BciT130I CCWGG 1 cut(s) 1263
BcnI CCSGG 2 cut(s) 1054, 1436
BfaI CTAG 4 cut(s) 102, 108, 836, 1112
BfmI CTRYAG 2 cut(s) 525, 1377
BfrI CTTAAG 1 cut(s) 1301
BisI GCNGC 8 cut(s) 335, 360, 369, 528, 742, 855, 1380, 1401
BlsI GCNGC 8 cut(s) 336, 361, 370, 529, 743, 856, 1381, 1402
Bme1390I CCNGG 3 cut(s) 1054, 1263, 1436
Bme18I GGWCC 3 cut(s) 248, 800, 965
BmeT110I CYCGRG 2 cut(s) 302, 1153
BmgT120I GGNCC 4 cut(s) 248, 800, 965, 1389
BmrFI CCNGG 3 cut(s) 1054, 1263, 1436
BmrI ACTGGG 1 cut(s) 331
BmuI ACTGGG 1 cut(s) 331
BoxI GACNNNNGTC 1 cut(s) 304
BplI GAGNNNNNCTC 2 cut(s) 754, 786
BpmI CTGGAG 2 cut(s) 174, 681
BpuEI CTTGAG 1 cut(s) 572
BpuMI CCSGG 2 cut(s) 1054, 1436
BsaI GGTCTC 3 cut(s) 87, 226, 1085
BsaJI CCNNGG 3 cut(s) 773, 820, 1404
BsaWI WCCGGW 2 cut(s) 221, 1307
BsaXI ACNNNNNCTCC 4 cut(s) 654, 684, 1065, 1095
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 263, 649, 779, 1342
Bse118I RCCGGY 2 cut(s) 967, 1307
Bse1I ACTGG 3 cut(s) 157, 326, 664
Bse3DI GCAATG 2 cut(s) 751, 864
BseAI TCCGGA 1 cut(s) 221
BseBI CCWGG 1 cut(s) 1263
BseDI CCNNGG 3 cut(s) 773, 820, 1404
BseLI CCNNNNNNNGG 5 cut(s) 78, 263, 649, 779, 1342
BseMI GCAATG 2 cut(s) 751, 864
BseMII CTCAG 5 cut(s) 46, 380, 515, 645, 963
BseNI ACTGG 3 cut(s) 157, 326, 664
BseRI GAGGAG 5 cut(s) 171, 282, 285, 408, 970
BseXI GCAGC 7 cut(s) 346, 346, 539, 728, 841, 1391, 1412
BshFI GGCC 1 cut(s) 1391
BshTI ACCGGT 1 cut(s) 1307
BsiHKAI GWGCWC 2 cut(s) 349, 418
BsiHKCI CYCGRG 2 cut(s) 302, 1153
BsiSI CCGG 5 cut(s) 222, 968, 1054, 1308, 1436
BslFI GGGAC 4 cut(s) 81, 846, 1093, 1170
BslI CCNNNNNNNGG 5 cut(s) 78, 263, 649, 779, 1342
BsmFI GGGAC 4 cut(s) 81, 846, 1093, 1170
BsnI GGCC 1 cut(s) 1391
Bso31I GGTCTC 3 cut(s) 87, 226, 1085
BsoBI CYCGRG 2 cut(s) 302, 1153
Bsp1286I GDGCHC 3 cut(s) 349, 418, 1023
Bsp13I TCCGGA 1 cut(s) 221
BspACI CCGC 7 cut(s) 99, 162, 177, 368, 817, 866, 1432
BspANI GGCC 1 cut(s) 1391
BspCNI CTCAG 5 cut(s) 45, 381, 514, 646, 964
BspEI TCCGGA 1 cut(s) 221
BspHI TCATGA 1 cut(s) 1140
BspMAI CTGCAG 2 cut(s) 529, 1381
BspPI GGATC 6 cut(s) 63, 213, 248, 443, 790, 833
BspTI CTTAAG 1 cut(s) 1301
BspTNI GGTCTC 3 cut(s) 87, 226, 1085
BsrDI GCAATG 2 cut(s) 751, 864
BsrFI RCCGGY 2 cut(s) 967, 1307
BsrI ACTGG 3 cut(s) 157, 326, 664
BssAI RCCGGY 2 cut(s) 967, 1307
BssECI CCNNGG 3 cut(s) 773, 820, 1404
BssSI CACGAG 1 cut(s) 342
BssT1I CCWWGG 2 cut(s) 773, 1404
Bst2BI CACGAG 1 cut(s) 342
Bst2UI CCWGG 1 cut(s) 1263
Bst4CI ACNGT 5 cut(s) 519, 628, 1070, 1081, 1124
Bst6I CTCTTC 1 cut(s) 445
BstAFI CTTAAG 1 cut(s) 1301
BstAPI GCANNNNNTGC 2 cut(s) 863, 1376
BstC8I GCNNGC 3 cut(s) 711, 739, 752
BstDEI CTNAG 7 cut(s) 32, 389, 464, 501, 648, 654, 972
BstDSI CCRYGG 1 cut(s) 820
BstHHI GCGC 1 cut(s) 196
BstMWI GCNNNNNNNGC 9 cut(s) 202, 365, 368, 422, 747, 863, 1376, 1388, 1397
BstNI CCWGG 1 cut(s) 1263
BstNSI RCATGY 2 cut(s) 697, 989
BstPAI GACNNNNGTC 1 cut(s) 304
BstSCI CCNGG 3 cut(s) 1052, 1261, 1434
BstSFI CTRYAG 2 cut(s) 525, 1377
BstV1I GCAGC 7 cut(s) 346, 346, 539, 728, 841, 1391, 1412
BstX2I RGATCY 3 cut(s) 218, 782, 838
BstXI CCANNNNNNTGG 1 cut(s) 696
BstYI RGATCY 3 cut(s) 218, 782, 838
BsuRI GGCC 1 cut(s) 1391
BtgI CCRYGG 1 cut(s) 820
BtgZI GCGATG 1 cut(s) 590
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 3 cut(s) 319, 522, 930
Cac8I GCNNGC 3 cut(s) 711, 739, 752
CaiI CAGNNNCTG 1 cut(s) 533
CciI TCATGA 1 cut(s) 1140
CfoI GCGC 1 cut(s) 196
Cfr10I RCCGGY 2 cut(s) 967, 1307
Cfr13I GGNCC 4 cut(s) 248, 800, 965, 1389
Csp6I GTAC 2 cut(s) 520, 1333
CspAI ACCGGT 1 cut(s) 1307
CviAII CATG 5 cut(s) 118, 434, 694, 986, 1141
CviQI GTAC 2 cut(s) 520, 1333
DdeI CTNAG 7 cut(s) 32, 389, 464, 501, 648, 654, 972
Eam1104I CTCTTC 1 cut(s) 445
EarI CTCTTC 1 cut(s) 445
EciI GGCGGA 2 cut(s) 192, 806
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 2 cut(s) 773, 1404
Eco24I GRGCYC 2 cut(s) 418, 1023
Eco31I GGTCTC 3 cut(s) 87, 226, 1085
Eco47I GGWCC 3 cut(s) 248, 800, 965
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 3 cut(s) 585, 812, 988
Eco88I CYCGRG 2 cut(s) 302, 1153
EcoICRI GAGCTC 1 cut(s) 416
EcoO109I RGGNCCY 2 cut(s) 248, 800
EcoRII CCWGG 1 cut(s) 1261
EcoT14I CCWWGG 2 cut(s) 773, 1404
EcoT38I GRGCYC 2 cut(s) 418, 1023
ErhI CCWWGG 2 cut(s) 773, 1404
FaeI CATG 5 cut(s) 121, 437, 697, 989, 1144
FaqI GGGAC 4 cut(s) 81, 846, 1093, 1170
FatI CATG 5 cut(s) 117, 433, 693, 985, 1140
FauI CCCGC 1 cut(s) 106
Fnu4HI GCNGC 8 cut(s) 335, 360, 369, 528, 742, 855, 1380, 1401
FriOI GRGCYC 2 cut(s) 418, 1023
Fsp4HI GCNGC 8 cut(s) 335, 360, 369, 528, 742, 855, 1380, 1401
FspBI CTAG 4 cut(s) 102, 108, 836, 1112
GlaI GCGC 1 cut(s) 195
GluI GCNGC 8 cut(s) 335, 360, 369, 528, 742, 855, 1380, 1401
GsuI CTGGAG 2 cut(s) 174, 681
HaeIII GGCC 1 cut(s) 1391
HapII CCGG 5 cut(s) 222, 968, 1054, 1308, 1436
HhaI GCGC 1 cut(s) 196
Hin1II CATG 5 cut(s) 121, 437, 697, 989, 1144
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HindIII AAGCTT 1 cut(s) 752
HinfI GANTC 8 cut(s) 290, 300, 634, 685, 1040, 1057, 1137, 1322
HpaII CCGG 5 cut(s) 222, 968, 1054, 1308, 1436
HphI GGTGA 4 cut(s) 105, 907, 914, 1421
Hpy166II GTNNAC 4 cut(s) 113, 522, 824, 1030
Hpy8I GTNNAC 4 cut(s) 113, 522, 824, 1030
Hpy99I CGWCG 2 cut(s) 367, 1201
HpyAV CCTTC 5 cut(s) 239, 257, 886, 956, 1127
HpyCH4III ACNGT 5 cut(s) 519, 628, 1070, 1081, 1124
HpyCH4IV ACGT 1 cut(s) 1257
HpyCH4V TGCA 6 cut(s) 527, 744, 857, 930, 1379, 1400
HpyF10VI GCNNNNNNNGC 9 cut(s) 202, 365, 368, 422, 747, 863, 1376, 1388, 1397
HpyF3I CTNAG 7 cut(s) 32, 389, 464, 501, 648, 654, 972
HpySE526I ACGT 1 cut(s) 1257
Hsp92II CATG 5 cut(s) 121, 437, 697, 989, 1144
HspAI GCGC 1 cut(s) 194
Kpn2I TCCGGA 1 cut(s) 221
LmnI GCTCC 6 cut(s) 352, 413, 421, 430, 957, 1416
Lsp1109I GCAGC 7 cut(s) 346, 346, 539, 728, 841, 1391, 1412
MaeI CTAG 4 cut(s) 102, 108, 836, 1112
MaeII ACGT 1 cut(s) 1257
MaeIII GTNAC 4 cut(s) 63, 182, 317, 1168
MboII GAAGA 8 cut(s) 150, 389, 432, 503, 901, 917, 1235, 1436
MflI RGATCY 3 cut(s) 218, 782, 838
MhlI GDGCHC 3 cut(s) 349, 418, 1023
MluCI AATT 1 cut(s) 210
MlyI GAGTC 1 cut(s) 294
MroI TCCGGA 1 cut(s) 221
MseI TTAA 7 cut(s) 149, 209, 327, 561, 1254, 1302, 1316
MslI CAYNNNNRTG 1 cut(s) 1449
MspA1I CMGCKG 2 cut(s) 337, 1295
MspCI CTTAAG 1 cut(s) 1301
MspI CCGG 5 cut(s) 222, 968, 1054, 1308, 1436
MspR9I CCNGG 3 cut(s) 1054, 1263, 1436
MvaI CCWGG 1 cut(s) 1263
MwoI GCNNNNNNNGC 9 cut(s) 202, 365, 368, 422, 747, 863, 1376, 1388, 1397
NciI CCSGG 2 cut(s) 1054, 1436
NlaIII CATG 5 cut(s) 121, 437, 697, 989, 1144
NmuCI GTSAC 3 cut(s) 63, 317, 1168
NspI RCATGY 2 cut(s) 697, 989
PagI TCATGA 1 cut(s) 1140
PciI ACATGT 1 cut(s) 693
PcsI WCGNNNNNNNCGW 1 cut(s) 1205
PfeI GAWTC 7 cut(s) 290, 634, 685, 1040, 1057, 1137, 1322
PinAI ACCGGT 1 cut(s) 1307
PkrI GCNGC 8 cut(s) 336, 361, 370, 529, 743, 856, 1381, 1402
PleI GAGTC 1 cut(s) 294
PpsI GAGTC 1 cut(s) 294
PpuMI RGGWCCY 2 cut(s) 248, 800
PscI ACATGT 1 cut(s) 693
PshAI GACNNNNGTC 1 cut(s) 304
Psp124BI GAGCTC 1 cut(s) 418
Psp1406I AACGTT 1 cut(s) 1257
Psp5II RGGWCCY 2 cut(s) 248, 800
Psp6I CCWGG 1 cut(s) 1261
PspGI CCWGG 1 cut(s) 1261
PspPI GGNCC 4 cut(s) 248, 800, 965, 1389
PspPPI RGGWCCY 2 cut(s) 248, 800
PstI CTGCAG 2 cut(s) 529, 1381
PstNI CAGNNNCTG 1 cut(s) 533
PsuI RGATCY 3 cut(s) 218, 782, 838
PvuII CAGCTG 2 cut(s) 337, 1295
RsaI GTAC 2 cut(s) 521, 1334
RsaNI GTAC 2 cut(s) 520, 1333
RseI CAYNNNNRTG 1 cut(s) 1449
SacI GAGCTC 1 cut(s) 418
SaqAI TTAA 7 cut(s) 149, 209, 327, 561, 1254, 1302, 1316
SatI GCNGC 8 cut(s) 335, 360, 369, 528, 742, 855, 1380, 1401
Sau96I GGNCC 4 cut(s) 248, 800, 965, 1389
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 3 cut(s) 1054, 1263, 1436
SduI GDGCHC 3 cut(s) 349, 418, 1023
SfcI CTRYAG 2 cut(s) 525, 1377
SinI GGWCC 3 cut(s) 248, 800, 965
SmiMI CAYNNNNRTG 1 cut(s) 1449
SmlI CTYRAG 2 cut(s) 587, 1301
SmoI CTYRAG 2 cut(s) 587, 1301
Sse9I AATT 1 cut(s) 210
SsiI CCGC 7 cut(s) 99, 162, 177, 368, 817, 866, 1432
SspMI CTAG 4 cut(s) 102, 108, 836, 1112
SstI GAGCTC 1 cut(s) 418
StyD4I CCNGG 3 cut(s) 1052, 1261, 1434
StyI CCWWGG 2 cut(s) 773, 1404
TaaI ACNGT 5 cut(s) 519, 628, 1070, 1081, 1124
TaiI ACGT 1 cut(s) 1260
TaqI TCGA 4 cut(s) 486, 637, 1131, 1275
TasI AATT 1 cut(s) 210
TatI WGTACW 1 cut(s) 519
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 7 cut(s) 290, 634, 685, 1040, 1057, 1137, 1322
Tru1I TTAA 7 cut(s) 149, 209, 327, 561, 1254, 1302, 1316
Tru9I TTAA 7 cut(s) 149, 209, 327, 561, 1254, 1302, 1316
TscAI CASTG 3 cut(s) 326, 529, 937
TseFI GTSAC 3 cut(s) 63, 317, 1168
TseI GCWGC 7 cut(s) 334, 359, 527, 741, 854, 1379, 1400
Tsp45I GTSAC 3 cut(s) 63, 317, 1168
TspDTI ATGAA 4 cut(s) 276, 422, 698, 904
TspGWI ACGGA 2 cut(s) 79, 339
TspRI CASTG 3 cut(s) 326, 529, 937
Vha464I CTTAAG 1 cut(s) 1301
VpaK11BI GGWCC 3 cut(s) 248, 800, 965
XceI RCATGY 2 cut(s) 697, 989
XspI CTAG 4 cut(s) 102, 108, 836, 1112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.