pycom10g08660

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002346
Physical Location & Seq
Forward (+)
11419092 .. 11419517
426 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g08660.1

Sequence Viewer

Length: 426 bp
ATGGAGGATTCACATCTGACCCGCATCCAATCCAATGGTGGCGATGCTGTTTACTGGGAAACCACAATCAATTCATTCCTTGATGATTACAGGCCTGTTGAAGGAATCATGATTGCACACTCAGGGCGTTCCGTGGTAACCCTATTCAGGTTTGGTGAAATGGCAATGAGCCATACCAAAACAAGGATGGAAGAAGCTTGGACAATTGAGGAGGTTGCATTTAATGTTCCAGGTTTGTCAATGGATTGCTTCATCCCCCCAGCTGACTTAAGATCGGGGACAATCAGTGAAGCGAGCGAGCTTCCACATGATGAAAGGGGAAAGGGTGGTGGGATTGCAGTAGCAGCACATCGGGCCAAAGTTGCTGCACTGGAGAAAGAGCACAATGCTAATGTTGATAGCCTGACCTGGAAGATGGAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

142

Amino Acids

15.58

Weight (kDa)

5.04

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 1 - 87 5.2e-41 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 22
AfiI CCNNNNNNNGG 3 cut(s) 101, 147, 183
AflII CTTAAG 1 cut(s) 268
AgsI TTSAA 1 cut(s) 101
AjnI CCWGG 2 cut(s) 229, 407
AluBI AGCT 3 cut(s) 197, 263, 301
AluI AGCT 3 cut(s) 197, 263, 301
Alw21I GWGCWC 1 cut(s) 384
AoxI GGCC 2 cut(s) 92, 354
ApeKI GCWGC 2 cut(s) 344, 365
AspS9I GGNCC 1 cut(s) 354
AsuHPI GGTGA 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 352, 356
BccI CCATC 2 cut(s) 181, 409
BciT130I CCWGG 2 cut(s) 231, 409
BfrI CTTAAG 1 cut(s) 268
BisI GCNGC 2 cut(s) 345, 366
BlsI GCNGC 2 cut(s) 346, 367
Bme1390I CCNGG 2 cut(s) 231, 409
BmgT120I GGNCC 1 cut(s) 354
BmrFI CCNGG 2 cut(s) 231, 409
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 2 cut(s) 33, 34
BmuI ACTGGG 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 392
BsaBI GATNNNNATC 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 147, 183
Bse1I ACTGG 2 cut(s) 59, 375
Bse3DI GCAATG 1 cut(s) 171
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 231, 409
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 3 cut(s) 24, 192, 252
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 3 cut(s) 101, 147, 183
BseMI GCAATG 1 cut(s) 171
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 2 cut(s) 59, 375
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 2 cut(s) 352, 356
BseYI CCCAGC 1 cut(s) 259
BsgI GTGCAG 1 cut(s) 351
BshFI GGCC 2 cut(s) 94, 356
BsiHKAI GWGCWC 1 cut(s) 384
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 3 cut(s) 101, 147, 183
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 2 cut(s) 94, 356
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 1 cut(s) 272
BspACI CCGC 1 cut(s) 22
BspANI GGCC 2 cut(s) 94, 356
BspCNI CTCAG 1 cut(s) 134
BspHI TCATGA 1 cut(s) 108
BspTI CTTAAG 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 171
BsrI ACTGG 2 cut(s) 59, 375
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 1 cut(s) 272
Bst2UI CCWGG 2 cut(s) 231, 409
BstAFI CTTAAG 1 cut(s) 268
BstC8I GCNNGC 2 cut(s) 295, 299
BstDEI CTNAG 1 cut(s) 121
BstDSI CCRYGG 1 cut(s) 132
BstEII GGTNACC 1 cut(s) 136
BstENI CCTNNNNNAGG 1 cut(s) 99
BstF5I GGATG 3 cut(s) 24, 192, 252
BstKTI GATC 1 cut(s) 275
BstMBI GATC 1 cut(s) 272
BstMWI GCNNNNNNNGC 3 cut(s) 344, 353, 362
BstNI CCWGG 2 cut(s) 231, 409
BstPI GGTNACC 1 cut(s) 136
BstSCI CCNGG 2 cut(s) 229, 407
BstV1I GCAGC 2 cut(s) 352, 356
BstXI CCANNNNNNTGG 1 cut(s) 35
BsuRI GGCC 2 cut(s) 94, 356
BtgI CCRYGG 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 57
BtsCI GGATG 3 cut(s) 24, 192, 252
BtsIMutI CAGTG 2 cut(s) 292, 368
Cac8I GCNNGC 2 cut(s) 295, 299
CciI TCATGA 1 cut(s) 108
Cfr13I GGNCC 1 cut(s) 354
CviAII CATG 2 cut(s) 109, 308
CviJI RGCY 7 cut(s) 94, 171, 197, 263, 301, 356, 402
CviKI_1 RGCY 7 cut(s) 94, 171, 197, 263, 301, 356, 402
DdeI CTNAG 1 cut(s) 121
DpnI GATC 1 cut(s) 274
DpnII GATC 1 cut(s) 272
Eco147I AGGCCT 1 cut(s) 94
Eco91I GGTNACC 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 99
EcoO65I GGTNACC 1 cut(s) 136
EcoRII CCWGG 2 cut(s) 229, 407
FaeI CATG 2 cut(s) 112, 311
FaiI YATR 3 cut(s) 110, 174, 309
FaqI GGGAC 1 cut(s) 292
FatI CATG 2 cut(s) 108, 307
FauI CCCGC 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 345, 366
FokI GGATG 3 cut(s) 11, 199, 239
Fsp4HI GCNGC 2 cut(s) 345, 366
GluI GCNGC 2 cut(s) 345, 366
GsaI CCCAGC 1 cut(s) 263
GsuI CTGGAG 1 cut(s) 392
HaeIII GGCC 2 cut(s) 94, 356
Hin1II CATG 2 cut(s) 112, 311
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 2 cut(s) 8, 105
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 1 cut(s) 52
HpyAV CCTTC 1 cut(s) 95
HpyCH4V TGCA 4 cut(s) 116, 218, 338, 368
HpyF10VI GCNNNNNNNGC 3 cut(s) 344, 353, 362
HpyF3I CTNAG 1 cut(s) 121
Hsp92II CATG 2 cut(s) 112, 311
Kzo9I GATC 1 cut(s) 272
Lsp1109I GCAGC 2 cut(s) 352, 356
LweI GCATC 2 cut(s) 33, 34
MaeIII GTNAC 1 cut(s) 136
MalI GATC 1 cut(s) 274
MboI GATC 1 cut(s) 272
MboII GAAGA 2 cut(s) 203, 424
MfeI CAATTG 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 384
MluCI AATT 2 cut(s) 70, 204
MnlI CCTC 2 cut(s) 202, 205
MseI TTAA 2 cut(s) 222, 269
MspA1I CMGCKG 1 cut(s) 263
MspCI CTTAAG 1 cut(s) 268
MspR9I CCNGG 2 cut(s) 231, 409
MunI CAATTG 1 cut(s) 204
MvaI CCWGG 2 cut(s) 231, 409
MwoI GCNNNNNNNGC 3 cut(s) 344, 353, 362
NdeII GATC 1 cut(s) 272
NlaIII CATG 2 cut(s) 112, 311
PagI TCATGA 1 cut(s) 108
PceI AGGCCT 1 cut(s) 94
PfeI GAWTC 2 cut(s) 8, 105
PkrI GCNGC 2 cut(s) 346, 367
Psp6I CCWGG 2 cut(s) 229, 407
PspEI GGTNACC 1 cut(s) 136
PspFI CCCAGC 1 cut(s) 259
PspGI CCWGG 2 cut(s) 229, 407
PspPI GGNCC 1 cut(s) 354
PvuII CAGCTG 1 cut(s) 263
SaqAI TTAA 2 cut(s) 222, 269
SatI GCNGC 2 cut(s) 345, 366
Sau3AI GATC 1 cut(s) 272
Sau96I GGNCC 1 cut(s) 354
ScrFI CCNGG 2 cut(s) 231, 409
SduI GDGCHC 1 cut(s) 384
SetI ASST 7 cut(s) 152, 199, 216, 235, 265, 303, 410
SfaNI GCATC 2 cut(s) 33, 34
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
Sse9I AATT 2 cut(s) 70, 204
SseBI AGGCCT 1 cut(s) 94
SsiI CCGC 1 cut(s) 22
StuI AGGCCT 1 cut(s) 94
StyD4I CCNGG 2 cut(s) 229, 407
TasI AATT 2 cut(s) 70, 204
TfiI GAWTC 2 cut(s) 8, 105
Tru1I TTAA 2 cut(s) 222, 269
Tru9I TTAA 2 cut(s) 222, 269
TscAI CASTG 2 cut(s) 292, 375
TseI GCWGC 2 cut(s) 344, 365
TspDTI ATGAA 3 cut(s) 63, 241, 327
TspGWI ACGGA 1 cut(s) 121
TspRI CASTG 2 cut(s) 292, 375
Vha464I CTTAAG 1 cut(s) 268
XagI CCTNNNNNAGG 1 cut(s) 99
XcmI CCANNNNNNNNNTGG 2 cut(s) 35, 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.