Rroxscaffold_7G00210980

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002346
Physical Location & Seq
Forward (+)
61385807 .. 61389030
3224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00210980.1

Sequence Viewer

Length: 888 bp
ATGGGAAAGGTTAGGATGGTAGCTTGTGAGTTTGAAACTGCCACCAAGGTGATGAAGACCAGAAACGCATCCAGATGTGCAGAGTCGGGTGGGTTTGTGCTCTGGCAGATGAATCCGGACATGTGGTATGTGGAGCTTGCTGTTGGGGGCAACAAAGTTCATGCTGGCTGCAATGGGAAGCTCGTTTGGAGACACACGCCGTGGCAGGGTGCACATACTGCGAAAGGGCCTGTGAGACCCTTGCGTCGTGCTCTTCAGGGTCTTGATCCAAGATCTACAGCGAGTATGTTTGCTGACGCCAGATGTATTGGAGAAAGAAAGGTCAATGGGGAGGATTGCTTCATCCTCAAGCTCTGTGCTGATCCTCAAACTCTGAAGGCCAGGAGTGAAGGCCCTGCAGAGATAATTAGACATGTGTTGTTTGGCTACTTCAGCCAGAAGACAGGGCTTCTTGTTCACATGGAGGACTCTCATTTGACCCGCATTCAATCCAATGGCGGTGATGCTGTTTACTGGGAAACCACAATCAATTCGTTCCTAGATGATTACAGACCTGTTGAGGGAATCATGATTGCGCACTCCGGGCGTTCTGTGGTCACCCTTTTCAGGTTCGGCGAGATGGCAATGAGCCATACCAAAACAAAGATGGAAGAAGCTTGGACAATTGAAGAGGTTGCATTTAATGTTCCAGGCTTGTCAATGGACTGCTTCATCCCCCCAGCTGACTTGAGATCTGAGGCAATAATGAGTGAAGCATGCGAGCTTCCTCATGATGAAAGGGGAAAGGGTGGCGGGATTGCAGCAGCAGTGCATCGGGCCAAAGTAGCTGCGCTGGAGAAAGAGCATAGTGCAAATGGTGATAGCATGAGCTGGAAGATGGAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

295

Amino Acids

32.64

Weight (kDa)

6.83

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 1 - 240 1e-120 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 243
Acc16I TGCGCA 1 cut(s) 576
AccIII TCCGGA 1 cut(s) 115
AciI CCGC 3 cut(s) 481, 498, 792
AclWI GGATC 2 cut(s) 260, 356
AcuI CTGAAG 3 cut(s) 239, 395, 415
AcyI GRCGYC 1 cut(s) 297
AdeI CACNNNGTG 1 cut(s) 201
AfiI CCNNNNNNNGG 3 cut(s) 206, 560, 606
AflIII ACRYGT 2 cut(s) 120, 412
AgsI TTSAA 3 cut(s) 35, 488, 668
AjnI CCWGG 2 cut(s) 380, 688
AleI CACNNNNGTG 1 cut(s) 47
AluBI AGCT 9 cut(s) 23, 136, 181, 352, 656, 722, 763, 827, 870
AluI AGCT 9 cut(s) 23, 136, 181, 352, 656, 722, 763, 827, 870
Alw21I GWGCWC 3 cut(s) 102, 214, 253
Alw26I GTCTC 2 cut(s) 184, 229
Alw44I GTGCAC 1 cut(s) 210
AlwI GGATC 2 cut(s) 260, 356
Aor13HI TCCGGA 1 cut(s) 115
AoxI GGCC 4 cut(s) 227, 378, 391, 816
ApaLI GTGCAC 1 cut(s) 210
ApeKI GCWGC 4 cut(s) 168, 800, 803, 827
AspLEI GCGC 2 cut(s) 577, 832
AspS9I GGNCC 3 cut(s) 227, 392, 816
AsuC2I CCSGG 1 cut(s) 583
AsuHPI GGTGA 4 cut(s) 61, 512, 589, 869
BaeGI GKGCMC 1 cut(s) 214
BbsI GAAGAC 2 cut(s) 62, 446
Bbv12I GWGCWC 3 cut(s) 102, 214, 253
BbvI GCAGC 4 cut(s) 155, 812, 814, 815
BccI CCATC 4 cut(s) 10, 613, 640, 871
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 2 cut(s) 382, 690
BcnI CCSGG 1 cut(s) 583
BcoDI GTCTC 2 cut(s) 184, 229
BfaI CTAG 1 cut(s) 539
BfmI CTRYAG 2 cut(s) 276, 396
BglII AGATCT 2 cut(s) 272, 731
BisI GCNGC 4 cut(s) 169, 801, 804, 828
BlsI GCNGC 4 cut(s) 170, 802, 805, 829
Bme1390I CCNGG 3 cut(s) 382, 583, 690
BmgT120I GGNCC 3 cut(s) 227, 392, 816
BmrFI CCNGG 3 cut(s) 382, 583, 690
BmrI ACTGGG 1 cut(s) 523
BmsI GCATC 3 cut(s) 77, 493, 820
BmuI ACTGGG 1 cut(s) 523
BpiI GAAGAC 2 cut(s) 62, 446
BpmI CTGGAG 1 cut(s) 854
BpuEI CTTGAG 2 cut(s) 332, 748
BpuMI CCSGG 1 cut(s) 583
BsaBI GATNNNNATC 1 cut(s) 881
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 2 cut(s) 45, 200
BsaWI WCCGGW 1 cut(s) 115
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 560, 606
Bse1I ACTGG 1 cut(s) 518
Bse3DI GCAATG 2 cut(s) 178, 630
Bse8I GATNNNNATC 1 cut(s) 881
BseAI TCCGGA 1 cut(s) 115
BseBI CCWGG 2 cut(s) 382, 690
BseDI CCNNGG 2 cut(s) 45, 200
BseGI GGATG 4 cut(s) 21, 68, 342, 711
BseJI GATNNNNATC 1 cut(s) 881
BseLI CCNNNNNNNGG 3 cut(s) 206, 560, 606
BseMI GCAATG 2 cut(s) 178, 630
BseMII CTCAG 1 cut(s) 726
BseNI ACTGG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 4 cut(s) 155, 812, 814, 815
BseYI CCCAGC 1 cut(s) 718
BsgI GTGCAG 1 cut(s) 99
BshFI GGCC 4 cut(s) 229, 380, 393, 818
BsiHKAI GWGCWC 3 cut(s) 102, 214, 253
BsiSI CCGG 2 cut(s) 116, 582
BslI CCNNNNNNNGG 3 cut(s) 206, 560, 606
BsmAI GTCTC 2 cut(s) 184, 229
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 4 cut(s) 229, 380, 393, 818
Bso31I GGTCTC 1 cut(s) 229
Bsp1286I GDGCHC 3 cut(s) 102, 214, 253
Bsp13I TCCGGA 1 cut(s) 115
Bsp143I GATC 4 cut(s) 265, 272, 361, 731
BspACI CCGC 3 cut(s) 481, 498, 792
BspANI GGCC 4 cut(s) 229, 380, 393, 818
BspCNI CTCAG 1 cut(s) 727
BspEI TCCGGA 1 cut(s) 115
BspHI TCATGA 2 cut(s) 567, 769
BspMAI CTGCAG 1 cut(s) 400
BspPI GGATC 2 cut(s) 260, 356
BspQI GCTCTTC 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 229
BsrDI GCAATG 2 cut(s) 178, 630
BsrI ACTGG 1 cut(s) 518
BssECI CCNNGG 2 cut(s) 45, 200
BssMI GATC 4 cut(s) 265, 272, 361, 731
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 2 cut(s) 382, 690
Bst6I CTCTTC 2 cut(s) 258, 663
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 218
BstC8I GCNNGC 4 cut(s) 138, 166, 757, 761
BstDEI CTNAG 1 cut(s) 735
BstDSI CCRYGG 1 cut(s) 200
BstEII GGTNACC 1 cut(s) 595
BstF5I GGATG 4 cut(s) 21, 68, 342, 711
BstHHI GCGC 2 cut(s) 577, 832
BstKTI GATC 4 cut(s) 268, 275, 364, 734
BstMAI GTCTC 2 cut(s) 184, 229
BstMBI GATC 4 cut(s) 265, 272, 361, 731
BstMWI GCNNNNNNNGC 4 cut(s) 218, 432, 583, 824
BstNI CCWGG 2 cut(s) 382, 690
BstNSI RCATGY 3 cut(s) 124, 416, 759
BstPI GGTNACC 1 cut(s) 595
BstSCI CCNGG 3 cut(s) 380, 581, 688
BstSFI CTRYAG 2 cut(s) 276, 396
BstSLI GKGCMC 1 cut(s) 214
BstV1I GCAGC 4 cut(s) 155, 812, 814, 815
BstV2I GAAGAC 2 cut(s) 62, 446
BstX2I RGATCY 2 cut(s) 272, 731
BstYI RGATCY 2 cut(s) 272, 731
BsuRI GGCC 4 cut(s) 229, 380, 393, 818
BtgI CCRYGG 1 cut(s) 200
BtsCI GGATG 4 cut(s) 21, 68, 342, 711
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 1 cut(s) 813
Cac8I GCNNGC 4 cut(s) 138, 166, 757, 761
CciI TCATGA 2 cut(s) 567, 769
CfoI GCGC 2 cut(s) 577, 832
Cfr13I GGNCC 3 cut(s) 227, 392, 816
CseI GACGC 2 cut(s) 233, 305
CviAII CATG 8 cut(s) 121, 161, 413, 460, 568, 756, 770, 865
DdeI CTNAG 1 cut(s) 735
DpnI GATC 4 cut(s) 267, 274, 363, 733
DpnII GATC 4 cut(s) 265, 272, 361, 731
DraIII CACNNNGTG 1 cut(s) 201
DrdI GACNNNNNNGTC 1 cut(s) 243
DseDI GACNNNNNNGTC 1 cut(s) 243
Eam1104I CTCTTC 2 cut(s) 258, 663
EarI CTCTTC 2 cut(s) 258, 663
Eco130I CCWWGG 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 229
Eco57I CTGAAG 3 cut(s) 239, 395, 415
Eco91I GGTNACC 1 cut(s) 595
EcoO109I RGGNCCY 2 cut(s) 227, 392
EcoO65I GGTNACC 1 cut(s) 595
EcoRII CCWGG 2 cut(s) 380, 688
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 8 cut(s) 124, 164, 416, 463, 571, 759, 773, 868
FatI CATG 8 cut(s) 120, 160, 412, 459, 567, 755, 769, 864
FauI CCCGC 2 cut(s) 488, 785
Fnu4HI GCNGC 4 cut(s) 169, 801, 804, 828
FokI GGATG 4 cut(s) 28, 55, 329, 698
Fsp4HI GCNGC 4 cut(s) 169, 801, 804, 828
FspBI CTAG 1 cut(s) 539
FspI TGCGCA 1 cut(s) 576
GlaI GCGC 2 cut(s) 576, 831
GluI GCNGC 4 cut(s) 169, 801, 804, 828
GsaI CCCAGC 1 cut(s) 722
GsuI CTGGAG 1 cut(s) 854
HaeIII GGCC 4 cut(s) 229, 380, 393, 818
HapII CCGG 2 cut(s) 116, 582
HgaI GACGC 2 cut(s) 233, 305
HhaI GCGC 2 cut(s) 577, 832
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 8 cut(s) 124, 164, 416, 463, 571, 759, 773, 868
Hin6I GCGC 2 cut(s) 575, 830
HinP1I GCGC 2 cut(s) 575, 830
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 4 cut(s) 83, 112, 467, 564
HpaII CCGG 2 cut(s) 116, 582
HphI GGTGA 4 cut(s) 61, 512, 589, 869
Hpy166II GTNNAC 3 cut(s) 212, 457, 511
Hpy188I TCNGA 2 cut(s) 375, 736
Hpy188III TCNNGA 5 cut(s) 72, 116, 263, 568, 770
Hpy8I GTNNAC 3 cut(s) 212, 457, 511
Hpy99I CGWCG 1 cut(s) 249
HpyAV CCTTC 2 cut(s) 370, 383
HpyCH4V TGCA 8 cut(s) 80, 171, 212, 398, 677, 800, 811, 851
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 432, 583, 824
HpyF3I CTNAG 1 cut(s) 735
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 8 cut(s) 124, 164, 416, 463, 571, 759, 773, 868
HspAI GCGC 2 cut(s) 575, 830
Kpn2I TCCGGA 1 cut(s) 115
Kzo9I GATC 4 cut(s) 265, 272, 361, 731
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 4 cut(s) 155, 812, 814, 815
LweI GCATC 3 cut(s) 77, 493, 820
MaeI CTAG 1 cut(s) 539
MaeIII GTNAC 1 cut(s) 595
MalI GATC 4 cut(s) 267, 274, 363, 733
MboI GATC 4 cut(s) 265, 272, 361, 731
MboII GAAGA 6 cut(s) 67, 245, 451, 662, 680, 886
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 2 cut(s) 272, 731
MhlI GDGCHC 3 cut(s) 102, 214, 253
MluCI AATT 3 cut(s) 405, 529, 663
MlyI GAGTC 2 cut(s) 92, 461
MnlI CCTC 8 cut(s) 325, 356, 375, 457, 553, 664, 730, 777
MroI TCCGGA 1 cut(s) 115
MseI TTAA 1 cut(s) 681
MslI CAYNNNNRTG 2 cut(s) 47, 73
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 2 cut(s) 116, 582
MspR9I CCNGG 3 cut(s) 382, 583, 690
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 2 cut(s) 382, 690
MwoI GCNNNNNNNGC 4 cut(s) 218, 432, 583, 824
NciI CCSGG 1 cut(s) 583
NdeII GATC 4 cut(s) 265, 272, 361, 731
NlaIII CATG 8 cut(s) 124, 164, 416, 463, 571, 759, 773, 868
NmuCI GTSAC 1 cut(s) 595
NsbI TGCGCA 1 cut(s) 576
NspI RCATGY 3 cut(s) 124, 416, 759
OliI CACNNNNGTG 1 cut(s) 47
PaeI GCATGC 1 cut(s) 759
PagI TCATGA 2 cut(s) 567, 769
PciI ACATGT 2 cut(s) 120, 412
PciSI GCTCTTC 1 cut(s) 258
PctI GAATGC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 112, 564
PkrI GCNGC 4 cut(s) 170, 802, 805, 829
PleI GAGTC 2 cut(s) 91, 461
PpsI GAGTC 2 cut(s) 91, 461
PscI ACATGT 2 cut(s) 120, 412
Psp6I CCWGG 2 cut(s) 380, 688
PspEI GGTNACC 1 cut(s) 595
PspFI CCCAGC 1 cut(s) 718
PspGI CCWGG 2 cut(s) 380, 688
PspPI GGNCC 3 cut(s) 227, 392, 816
PstI CTGCAG 1 cut(s) 400
PsuI RGATCY 2 cut(s) 272, 731
PvuII CAGCTG 1 cut(s) 722
RseI CAYNNNNRTG 2 cut(s) 47, 73
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 1 cut(s) 681
SatI GCNGC 4 cut(s) 169, 801, 804, 828
Sau3AI GATC 4 cut(s) 265, 272, 361, 731
Sau96I GGNCC 3 cut(s) 227, 392, 816
SchI GAGTC 2 cut(s) 92, 461
ScrFI CCNGG 3 cut(s) 382, 583, 690
SduI GDGCHC 3 cut(s) 102, 214, 253
SfaNI GCATC 3 cut(s) 77, 493, 820
SfcI CTRYAG 2 cut(s) 276, 396
SmiMI CAYNNNNRTG 2 cut(s) 47, 73
SmlI CTYRAG 2 cut(s) 347, 727
SmoI CTYRAG 2 cut(s) 347, 727
SphI GCATGC 1 cut(s) 759
Sse9I AATT 3 cut(s) 405, 529, 663
SsiI CCGC 3 cut(s) 481, 498, 792
SspMI CTAG 1 cut(s) 539
StyD4I CCNGG 3 cut(s) 380, 581, 688
StyI CCWWGG 1 cut(s) 45
TasI AATT 3 cut(s) 405, 529, 663
TfiI GAWTC 2 cut(s) 112, 564
Tru1I TTAA 1 cut(s) 681
Tru9I TTAA 1 cut(s) 681
TscAI CASTG 1 cut(s) 813
TseFI GTSAC 1 cut(s) 595
TseI GCWGC 4 cut(s) 168, 800, 803, 827
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 6 cut(s) 68, 125, 149, 331, 700, 789
TspRI CASTG 1 cut(s) 813
VneI GTGCAC 1 cut(s) 210
XceI RCATGY 3 cut(s) 124, 416, 759
XcmI CCANNNNNNNNNTGG 1 cut(s) 643
XspI CTAG 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.