FvH4_2g33400

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002346
Physical Location & Seq
Reverse (-)
25063277 .. 25066437
3161 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33400.t1

Sequence Viewer

Length: 1419 bp
ATGGAGAGGAAACAGGGGTTCTTTTCGTCCCTTAAGGAGGATGTGGTGAGGGGTCTGTCGCCGGCGAGGTCCAGGTCGAAGAGTCCGGCGAGAAGTGCGTCGCCAATGTCGGGTCTGCTTCGGAGAAGGAGGAGGCACCACGTGGCGGAGCAGCTGATGGTGGCGAGATCCGGGGGGCTGAGGCCGCCGGCGGAGGCGCTGTCGCCGTTGAAGGAAGGGCCGGATGAGACGGACGGCGAGGATTTGAAGATGGAGGGGAGGTGGGGCCACTGGATGAAGGGCCAGCTGTGTAGGGCTCCGGCCGTCTCGTGCTCGTCGTCGGCGTATAAGAGGTCGGATCTGAGGCTGCTGCTTGGGGTTTTGGGTGCACCGTTGGGGCCTGTGCACGTTAGCACAGTCGATCCTTTCCCTCATCTCAGTATTAAGGACACCCCCATTGAAACTTCGTCTGCTCAGTATATATTGCAACAATATACAGCAGCATCCGGGGGGCAAAAGCTTCAGAACTCGATTCGCAATGCTTATGCTATGGGAAAGGTGAGGATGGTAGCTTCTGAGTTTGAGACAGCTGGCAAGGTTGTGAAGAGTCGCAATTCCTCCAAAGCAGCAGATTCAGGTGGCTTTGTCCTGTGGCAAATGCATCCAGATATGTGGTATGTGGAACTTGCCATTGGCGGCAGCAAGGTTCATGCTGGTTGCAATGGAAAGCTTGTCTGGAGGCACACACCATGGCTTGGTGCGCACACTGCTAAAGGGCCTGTTAGACCCTTGCGGCGTGCACTTCAGGGACTTGACCCGAGAACCACTGCTAGCATGTTCACCAACTCAAGATGCATTGGTGAAAAGAAGATCAATGGTGAGGATTGCTTCATCCTGAAGCTTAATACAGATCCATTGACTTTAAAAGCCAGGAGTGAAGGGCCTGCGGAGATTATAAGACATGTTTTATTTGGCTACTTCAGCCAGAGAACAGGTCTTCTAATACACTTGGAAGATTCTCATTTGACCCGCATTCAGAATCATGGAGGAGATGCTGTCTACTGGGAAACCACAATTAATTCATTCCTTGATGATTATAGGCCTGTGGAGGGAATTATGATCGCTCACTCAGGGCGTTCTGTAGTTACTCTTTTCAGGTTTGGAGAAACTGCAATGAGCCATACGAAGACAAGGATGGAAGAAGCTTGGTCAATTGAGGAAGTGGCATTCAATGTCCCTGGGTTGTCAGTAGACTGTTTTATCCCCCCTGCGGAGCTAAGATTTGCTTCTATGAGTGAAACCAGTGAGCTGCCTCAGGGTCAGACTGTAAAGAACACCATGGCAGGAGCTGCATATCGCTCCAAAGTTGCTGCACTTGATAAGTCTCATGATGCCAACGTCAGTAATATTGTGTGGAAGATGGGGAATATGGATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

473

Amino Acids

51.98

Weight (kDa)

9.45

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 175 - 416 4.8e-120 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 935
Acc16I TGCGCA 1 cut(s) 741
AccB1I GGYRCC 1 cut(s) 135
AccB7I CCANNNNNTGG 1 cut(s) 734
AccI GTMKAC 2 cut(s) 1038, 1230
AciI CCGC 8 cut(s) 146, 185, 191, 675, 772, 926, 1009, 1250
AclWI GGATC 4 cut(s) 162, 345, 395, 884
AcoI YGGCCR 1 cut(s) 300
AcuI CTGAAG 4 cut(s) 485, 767, 896, 943
AcvI CACGTG 1 cut(s) 142
AdeI CACNNNGTG 1 cut(s) 142
AfiI CCNNNNNNNGG 6 cut(s) 37, 110, 145, 734, 1088, 1249
AflII CTTAAG 1 cut(s) 32
AflIII ACRYGT 1 cut(s) 940
AgsI TTSAA 4 cut(s) 211, 247, 440, 1210
AjnI CCWGG 3 cut(s) 71, 908, 1216
AjuI GAANNNNNNNTTGG 2 cut(s) 654, 686
Alw21I GWGCWC 4 cut(s) 314, 370, 387, 781
Alw26I GTCTC 4 cut(s) 221, 310, 557, 1368
Alw44I GTGCAC 3 cut(s) 366, 383, 777
AlwI GGATC 4 cut(s) 162, 345, 395, 884
AlwNI CAGNNNCTG 1 cut(s) 1328
Ama87I CYCGRG 1 cut(s) 796
AoxI GGCC 9 cut(s) 182, 218, 265, 280, 300, 377, 755, 920, 1079
ApaLI GTGCAC 3 cut(s) 366, 383, 777
ApeKI GCWGC 9 cut(s) 151, 346, 349, 479, 605, 678, 1288, 1328, 1349
AseI ATTAAT 1 cut(s) 1056
AspLEI GCGC 2 cut(s) 199, 742
AspS9I GGNCC 7 cut(s) 69, 218, 265, 280, 377, 755, 920
AsuC2I CCSGG 2 cut(s) 172, 487
AsuHPI GGTGA 5 cut(s) 58, 550, 811, 851, 869
AsuNHI GCTAGC 1 cut(s) 809
AvaI CYCGRG 1 cut(s) 796
AvaII GGWCC 1 cut(s) 69
AxyI CCTNAGG 1 cut(s) 1293
BaeGI GKGCMC 3 cut(s) 370, 387, 781
BanI GGYRCC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 298
BauI CACGAG 1 cut(s) 307
BbrPI CACGTG 1 cut(s) 142
BbsI GAAGAC 2 cut(s) 968, 1172
Bbv12I GWGCWC 4 cut(s) 314, 370, 387, 781
BbvCI CCTCAGC 1 cut(s) 179
BbvI GCAGC 9 cut(s) 163, 333, 336, 491, 617, 690, 1275, 1315, 1336
BccI CCATC 5 cut(s) 151, 244, 538, 1168, 1393
BceAI ACGGC 3 cut(s) 190, 250, 287
BciT130I CCWGG 3 cut(s) 73, 910, 1218
BcnI CCSGG 2 cut(s) 172, 487
BcoDI GTCTC 4 cut(s) 221, 310, 557, 1368
BfaI CTAG 1 cut(s) 810
BfmI CTRYAG 1 cut(s) 1119
BfoI RGCGCY 1 cut(s) 200
BfrI CTTAAG 1 cut(s) 32
Bme1390I CCNGG 5 cut(s) 73, 172, 487, 910, 1218
Bme18I GGWCC 1 cut(s) 69
BmeT110I CYCGRG 1 cut(s) 796
BmgT120I GGNCC 7 cut(s) 69, 218, 265, 280, 377, 755, 920
BmiI GGNNCC 4 cut(s) 137, 266, 297, 378
BmrFI CCNGG 5 cut(s) 73, 172, 487, 910, 1218
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 5 cut(s) 491, 649, 821, 1021, 1360
BmtI GCTAGC 1 cut(s) 813
BmuI ACTGGG 1 cut(s) 1051
BpiI GAAGAC 2 cut(s) 968, 1172
BpmI CTGGAG 1 cut(s) 736
Bpu10I CCTNAGC 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 811
BpuMI CCSGG 2 cut(s) 172, 487
BsaAI YACGTR 1 cut(s) 142
BsaJI CCNNGG 6 cut(s) 171, 486, 728, 1216, 1217, 1317
BsaXI ACNNNNNCTCC 6 cut(s) 185, 215, 245, 275, 1020, 1050
Bsc4I CCNNNNNNNGG 6 cut(s) 37, 110, 145, 734, 1088, 1249
Bse118I RCCGGY 2 cut(s) 61, 187
Bse1I ACTGG 3 cut(s) 275, 1046, 1281
Bse21I CCTNAGG 1 cut(s) 1293
Bse3DI GCAATG 3 cut(s) 523, 706, 1158
BseBI CCWGG 3 cut(s) 73, 910, 1218
BseDI CCNNGG 6 cut(s) 171, 486, 728, 1216, 1217, 1317
BseGI GGATG 9 cut(s) 46, 229, 279, 482, 549, 640, 870, 1179, 1417
BseLI CCNNNNNNNGG 6 cut(s) 37, 110, 145, 734, 1088, 1249
BseMI GCAATG 3 cut(s) 523, 706, 1158
BseMII CTCAG 7 cut(s) 170, 332, 430, 467, 546, 1122, 1307
BseNI ACTGG 3 cut(s) 275, 1046, 1281
BseRI GAGGAG 2 cut(s) 145, 1041
BseSI GKGCMC 3 cut(s) 370, 387, 781
BseX3I CGGCCG 1 cut(s) 300
BseXI GCAGC 9 cut(s) 163, 333, 336, 491, 617, 690, 1275, 1315, 1336
BsgI GTGCAG 1 cut(s) 1335
Bsh1285I CGRYCG 1 cut(s) 303
BshFI GGCC 9 cut(s) 184, 220, 267, 282, 302, 379, 757, 922, 1081
BshNI GGYRCC 1 cut(s) 135
BsiEI CGRYCG 1 cut(s) 303
BsiHKAI GWGCWC 4 cut(s) 314, 370, 387, 781
BsiHKCI CYCGRG 1 cut(s) 796
BsiSI CCGG 7 cut(s) 62, 86, 171, 188, 221, 299, 486
BslFI GGGAC 3 cut(s) 13, 801, 1199
BslI CCNNNNNNNGG 6 cut(s) 37, 110, 145, 734, 1088, 1249
BsmAI GTCTC 4 cut(s) 221, 310, 557, 1368
BsmBI CGTCTC 2 cut(s) 221, 310
BsmFI GGGAC 3 cut(s) 13, 801, 1199
BsmI GAATGC 2 cut(s) 1011, 1205
BsnI GGCC 9 cut(s) 184, 220, 267, 282, 302, 379, 757, 922, 1081
BsoBI CYCGRG 1 cut(s) 796
Bsp1286I GDGCHC 5 cut(s) 298, 314, 370, 387, 781
Bsp143I GATC 6 cut(s) 167, 337, 400, 849, 889, 1098
Bsp19I CCATGG 2 cut(s) 728, 1317
BspACI CCGC 8 cut(s) 146, 185, 191, 675, 772, 926, 1009, 1250
BspANI GGCC 9 cut(s) 184, 220, 267, 282, 302, 379, 757, 922, 1081
BspCNI CTCAG 7 cut(s) 171, 333, 429, 466, 547, 1121, 1306
BspHI TCATGA 1 cut(s) 1366
BspLI GGNNCC 4 cut(s) 137, 266, 297, 378
BspOI GCTAGC 1 cut(s) 813
BspPI GGATC 4 cut(s) 162, 345, 395, 884
BspT107I GGYRCC 1 cut(s) 135
BspTI CTTAAG 1 cut(s) 32
BsrDI GCAATG 3 cut(s) 523, 706, 1158
BsrFI RCCGGY 2 cut(s) 61, 187
BsrI ACTGG 3 cut(s) 275, 1046, 1281
BssAI RCCGGY 2 cut(s) 61, 187
BssECI CCNNGG 6 cut(s) 171, 486, 728, 1216, 1217, 1317
BssMI GATC 6 cut(s) 167, 337, 400, 849, 889, 1098
BssSI CACGAG 1 cut(s) 307
BssT1I CCWWGG 2 cut(s) 728, 1317
Bst2BI CACGAG 1 cut(s) 307
Bst2UI CCWGG 3 cut(s) 73, 910, 1218
Bst4CI ACNGT 4 cut(s) 372, 397, 1235, 1306
Bst6I CTCTTC 2 cut(s) 74, 578
BstAFI CTTAAG 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 1328
BstBAI YACGTR 1 cut(s) 142
BstC8I GCNNGC 7 cut(s) 63, 189, 284, 571, 777, 811, 924
BstDEI CTNAG 8 cut(s) 179, 341, 416, 453, 555, 1108, 1256, 1293
BstDSI CCRYGG 2 cut(s) 728, 1317
BstENI CCTNNNNNAGG 1 cut(s) 35
BstF5I GGATG 9 cut(s) 46, 229, 279, 482, 549, 640, 870, 1179, 1417
BstH2I RGCGCY 1 cut(s) 200
BstHHI GCGC 2 cut(s) 199, 742
BstKTI GATC 6 cut(s) 170, 340, 403, 852, 892, 1101
BstMAI GTCTC 4 cut(s) 221, 310, 557, 1368
BstMBI GATC 6 cut(s) 167, 337, 400, 849, 889, 1098
BstMCI CGRYCG 1 cut(s) 303
BstMWI GCNNNNNNNGC 6 cut(s) 95, 184, 739, 746, 960, 1328
BstNI CCWGG 3 cut(s) 73, 910, 1218
BstNSI RCATGY 2 cut(s) 817, 944
BstSCI CCNGG 5 cut(s) 71, 170, 485, 908, 1216
BstSFI CTRYAG 1 cut(s) 1119
BstSLI GKGCMC 3 cut(s) 370, 387, 781
BstV1I GCAGC 9 cut(s) 163, 333, 336, 491, 617, 690, 1275, 1315, 1336
BstV2I GAAGAC 2 cut(s) 968, 1172
BstX2I RGATCY 3 cut(s) 167, 337, 889
BstXI CCANNNNNNTGG 1 cut(s) 651
BstYI RGATCY 3 cut(s) 167, 337, 889
BstZI CGGCCG 1 cut(s) 300
Bsu36I CCTNAGG 1 cut(s) 1293
BsuRI GGCC 9 cut(s) 184, 220, 267, 282, 302, 379, 757, 922, 1081
BtgI CCRYGG 2 cut(s) 728, 1317
BtsCI GGATG 9 cut(s) 46, 229, 279, 482, 549, 640, 870, 1179, 1417
BtsI GCAGTG 2 cut(s) 744, 804
BtsIMutI CAGTG 4 cut(s) 268, 744, 804, 1288
Cac8I GCNNGC 7 cut(s) 63, 189, 284, 571, 777, 811, 924
CaiI CAGNNNCTG 1 cut(s) 1328
CciI TCATGA 1 cut(s) 1366
CfoI GCGC 2 cut(s) 199, 742
Cfr10I RCCGGY 2 cut(s) 61, 187
Cfr13I GGNCC 7 cut(s) 69, 218, 265, 280, 377, 755, 920
CseI GACGC 1 cut(s) 87
CviAII CATG 7 cut(s) 689, 729, 814, 941, 1022, 1318, 1367
DdeI CTNAG 8 cut(s) 179, 341, 416, 453, 555, 1108, 1256, 1293
DpnI GATC 6 cut(s) 169, 339, 402, 851, 891, 1100
DpnII GATC 6 cut(s) 167, 337, 400, 849, 889, 1098
DraI TTTAAA 1 cut(s) 903
DraIII CACNNNGTG 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 300
EagI CGGCCG 1 cut(s) 300
Eam1104I CTCTTC 2 cut(s) 74, 578
EarI CTCTTC 2 cut(s) 74, 578
EciI GGCGGA 2 cut(s) 161, 206
EclXI CGGCCG 1 cut(s) 300
Eco130I CCWWGG 2 cut(s) 728, 1317
Eco147I AGGCCT 1 cut(s) 1081
Eco24I GRGCYC 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 69
Eco52I CGGCCG 1 cut(s) 300
Eco57I CTGAAG 4 cut(s) 485, 767, 896, 943
Eco72I CACGTG 1 cut(s) 142
Eco81I CCTNAGG 1 cut(s) 1293
Eco88I CYCGRG 1 cut(s) 796
EcoNI CCTNNNNNAGG 1 cut(s) 35
EcoO109I RGGNCCY 3 cut(s) 377, 755, 920
EcoRII CCWGG 3 cut(s) 71, 908, 1216
EcoT14I CCWWGG 2 cut(s) 728, 1317
EcoT22I ATGCAT 2 cut(s) 642, 836
EcoT38I GRGCYC 1 cut(s) 298
ErhI CCWWGG 2 cut(s) 728, 1317
Esp3I CGTCTC 2 cut(s) 221, 310
FaeI CATG 7 cut(s) 692, 732, 817, 944, 1025, 1321, 1370
FalI AAGNNNNNCTT 2 cut(s) 1249, 1281
FaqI GGGAC 3 cut(s) 13, 801, 1199
FatI CATG 7 cut(s) 688, 728, 813, 940, 1021, 1317, 1366
FauI CCCGC 1 cut(s) 1016
FblI GTMKAC 2 cut(s) 1038, 1230
FokI GGATG 8 cut(s) 53, 236, 286, 469, 556, 627, 857, 1186
FriOI GRGCYC 1 cut(s) 298
FspAI RTGCGCAY 1 cut(s) 741
FspBI CTAG 1 cut(s) 810
FspI TGCGCA 1 cut(s) 741
GlaI GCGC 2 cut(s) 198, 741
GsuI CTGGAG 1 cut(s) 736
HaeII RGCGCY 1 cut(s) 200
HaeIII GGCC 9 cut(s) 184, 220, 267, 282, 302, 379, 757, 922, 1081
HapII CCGG 7 cut(s) 62, 86, 171, 188, 221, 299, 486
HgaI GACGC 1 cut(s) 87
HhaI GCGC 2 cut(s) 199, 742
Hin1II CATG 7 cut(s) 692, 732, 817, 944, 1025, 1321, 1370
Hin6I GCGC 2 cut(s) 197, 740
HinP1I GCGC 2 cut(s) 197, 740
HindIII AAGCTT 4 cut(s) 497, 707, 878, 1182
HinfI GANTC 6 cut(s) 82, 511, 586, 611, 995, 1018
HpaII CCGG 7 cut(s) 62, 86, 171, 188, 221, 299, 486
HphI GGTGA 5 cut(s) 58, 550, 811, 851, 869
Hpy166II GTNNAC 6 cut(s) 368, 385, 779, 819, 1039, 1231
Hpy188I TCNGA 7 cut(s) 123, 337, 342, 504, 556, 1017, 1302
Hpy188III TCNNGA 5 cut(s) 644, 715, 828, 874, 1367
Hpy8I GTNNAC 6 cut(s) 368, 385, 779, 819, 1039, 1231
Hpy99I CGWCG 3 cut(s) 103, 319, 322
HpyAV CCTTC 5 cut(s) 120, 205, 209, 271, 911
HpyCH4III ACNGT 4 cut(s) 372, 397, 1235, 1306
HpyCH4IV ACGT 3 cut(s) 141, 387, 1377
HpyF10VI GCNNNNNNNGC 6 cut(s) 95, 184, 739, 746, 960, 1328
HpyF3I CTNAG 8 cut(s) 179, 341, 416, 453, 555, 1108, 1256, 1293
HpySE526I ACGT 3 cut(s) 141, 387, 1377
Hsp92II CATG 7 cut(s) 692, 732, 817, 944, 1025, 1321, 1370
HspAI GCGC 2 cut(s) 197, 740
KroI GCCGGC 2 cut(s) 61, 187
KroNI GCCGGC 2 cut(s) 63, 189
Kzo9I GATC 6 cut(s) 167, 337, 400, 849, 889, 1098
LmnI GCTCC 5 cut(s) 148, 301, 1252, 1325, 1343
Lsp1109I GCAGC 9 cut(s) 163, 333, 336, 491, 617, 690, 1275, 1315, 1336
LweI GCATC 5 cut(s) 491, 649, 821, 1021, 1360
MaeI CTAG 1 cut(s) 810
MaeII ACGT 3 cut(s) 141, 387, 1377
MaeIII GTNAC 1 cut(s) 1123
MalI GATC 6 cut(s) 169, 339, 402, 851, 891, 1100
MboI GATC 6 cut(s) 167, 337, 400, 849, 889, 1098
MboII GAAGA 9 cut(s) 91, 259, 595, 859, 968, 1004, 1177, 1190, 1408
MfeI CAATTG 1 cut(s) 1191
MflI RGATCY 3 cut(s) 167, 337, 889
MhlI GDGCHC 5 cut(s) 298, 314, 370, 387, 781
MluCI AATT 5 cut(s) 592, 1053, 1057, 1092, 1191
MlyI GAGTC 2 cut(s) 91, 595
MmeI TCCRAC 1 cut(s) 315
Mph1103I ATGCAT 2 cut(s) 642, 836
MreI CGCCGGCG 2 cut(s) 61, 187
MroNI GCCGGC 2 cut(s) 61, 187
MseI TTAA 5 cut(s) 33, 423, 882, 902, 1056
MspA1I CMGCKG 3 cut(s) 154, 286, 569
MspCI CTTAAG 1 cut(s) 32
MspI CCGG 7 cut(s) 62, 86, 171, 188, 221, 299, 486
MspR9I CCNGG 5 cut(s) 73, 172, 487, 910, 1218
MunI CAATTG 1 cut(s) 1191
Mva1269I GAATGC 2 cut(s) 1011, 1205
MvaI CCWGG 3 cut(s) 73, 910, 1218
MwoI GCNNNNNNNGC 6 cut(s) 95, 184, 739, 746, 960, 1328
NaeI GCCGGC 2 cut(s) 63, 189
NciI CCSGG 2 cut(s) 172, 487
NcoI CCATGG 2 cut(s) 728, 1317
NdeII GATC 6 cut(s) 167, 337, 400, 849, 889, 1098
NgoMIV GCCGGC 2 cut(s) 61, 187
NheI GCTAGC 1 cut(s) 809
NlaIII CATG 7 cut(s) 692, 732, 817, 944, 1025, 1321, 1370
NlaIV GGNNCC 4 cut(s) 137, 266, 297, 378
NsbI TGCGCA 1 cut(s) 741
NsiI ATGCAT 2 cut(s) 642, 836
NspI RCATGY 2 cut(s) 817, 944
PagI TCATGA 1 cut(s) 1366
PasI CCCWGGG 1 cut(s) 1217
PceI AGGCCT 1 cut(s) 1081
PciI ACATGT 1 cut(s) 940
PcsI WCGNNNNNNNCGW 2 cut(s) 314, 320
PctI GAATGC 2 cut(s) 1011, 1205
PdiI GCCGGC 2 cut(s) 63, 189
PfeI GAWTC 4 cut(s) 511, 611, 995, 1018
PflMI CCANNNNNTGG 1 cut(s) 734
PleI GAGTC 2 cut(s) 90, 594
PmaCI CACGTG 1 cut(s) 142
PmlI CACGTG 1 cut(s) 142
PpsI GAGTC 2 cut(s) 90, 594
Ppu21I YACGTR 1 cut(s) 142
PscI ACATGT 1 cut(s) 940
PshBI ATTAAT 1 cut(s) 1056
PsiI TTATAA 1 cut(s) 935
Psp6I CCWGG 3 cut(s) 71, 908, 1216
PspCI CACGTG 1 cut(s) 142
PspGI CCWGG 3 cut(s) 71, 908, 1216
PspN4I GGNNCC 4 cut(s) 137, 266, 297, 378
PspPI GGNCC 7 cut(s) 69, 218, 265, 280, 377, 755, 920
PstNI CAGNNNCTG 1 cut(s) 1328
PsuI RGATCY 3 cut(s) 167, 337, 889
PvuII CAGCTG 3 cut(s) 154, 286, 569
SaqAI TTAA 5 cut(s) 33, 423, 882, 902, 1056
Sau3AI GATC 6 cut(s) 167, 337, 400, 849, 889, 1098
Sau96I GGNCC 7 cut(s) 69, 218, 265, 280, 377, 755, 920
SchI GAGTC 2 cut(s) 91, 595
ScrFI CCNGG 5 cut(s) 73, 172, 487, 910, 1218
SduI GDGCHC 5 cut(s) 298, 314, 370, 387, 781
SfaNI GCATC 5 cut(s) 491, 649, 821, 1021, 1360
SfcI CTRYAG 1 cut(s) 1119
SgrAI CRCCGGYG 2 cut(s) 61, 187
SinI GGWCC 1 cut(s) 69
SmlI CTYRAG 2 cut(s) 32, 826
SmoI CTYRAG 2 cut(s) 32, 826
Sse9I AATT 5 cut(s) 592, 1053, 1057, 1092, 1191
SseBI AGGCCT 1 cut(s) 1081
SsiI CCGC 8 cut(s) 146, 185, 191, 675, 772, 926, 1009, 1250
SspI AATATT 1 cut(s) 1387
SspMI CTAG 1 cut(s) 810
StuI AGGCCT 1 cut(s) 1081
StyD4I CCNGG 5 cut(s) 71, 170, 485, 908, 1216
StyI CCWWGG 2 cut(s) 728, 1317
TaaI ACNGT 4 cut(s) 372, 397, 1235, 1306
TaiI ACGT 3 cut(s) 144, 390, 1380
TaqI TCGA 3 cut(s) 77, 399, 509
TasI AATT 5 cut(s) 592, 1053, 1057, 1092, 1191
TauI GCSGC 3 cut(s) 187, 678, 775
TfiI GAWTC 4 cut(s) 511, 611, 995, 1018
Tru1I TTAA 5 cut(s) 33, 423, 882, 902, 1056
Tru9I TTAA 5 cut(s) 33, 423, 882, 902, 1056
TscAI CASTG 4 cut(s) 275, 751, 811, 1288
TseI GCWGC 9 cut(s) 151, 346, 349, 479, 605, 678, 1288, 1328, 1349
TspDTI ATGAA 4 cut(s) 290, 677, 859, 1050
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 4 cut(s) 275, 751, 811, 1288
Van91I CCANNNNNTGG 1 cut(s) 734
Vha464I CTTAAG 1 cut(s) 32
VneI GTGCAC 3 cut(s) 366, 383, 777
VpaK11BI GGWCC 1 cut(s) 69
VspI ATTAAT 1 cut(s) 1056
XagI CCTNNNNNAGG 1 cut(s) 35
XceI RCATGY 2 cut(s) 817, 944
XmiI GTMKAC 2 cut(s) 1038, 1230
XspI CTAG 1 cut(s) 810
Zsp2I ATGCAT 2 cut(s) 642, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.