Rh6BG060800

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002346
Physical Location & Seq
Reverse (-)
9640089 .. 9643537
3449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG060800.1

Sequence Viewer

Length: 1434 bp
ATGGAGAAGAAGCAAGGGTTCTTCTCTGCTCTTAAAGACGAGGTGGTTCGCGGCCTCAGCCCCTCCCGCTCCCGCTCCCGCGCCACCAGCCCCGCCCGCTCCGCCTCCCCCATGTCGGGTCTGCTCCGCCGCAAGAAGCACCACACCCACCACGGCCACGGCCAGCTGGTCACGCAGCCGGAGCCCTTGATCGGGAGATCCGGCAGCCTGAGGCCGGTCATGGAGGGTCCGGATCTGGACGGCGGGGAAGTCGGGGAGTCGAAGCGGGTCGGGTCGGGTCTGGGCCAGTGGATGCGCGGGCAGCTTTCCCGGACTCCTTCGGTGAGTTCGGTTGAGCAGAACAACCGGAGGTCTGATCTGAGACTCTTGCTCGGAGTCATGGGAGCTCCGCTCGCCCCCCTCCACGTCAGCACCTCCGACCCTCTCCCTCACCTCAGCATCAAGGACACTCCCATCGAAACTTCGTCTGCCCAGTACATATTGCAACAGTACACAGCGGCATCTGGTGGGCAGAAGCTGCAGAACTCGATTAAAAATGCTTATGCGATGGGAAAGGTTAGGATGGTAGCTTCTGAGTTTGAAACTGCCACCAAGGTGATGAAGACCAGAAACGCATCTAGATGTGCAGAGTCGGGTGGGTTTGTGCTCTGGCAGATGAATCCGGACATGTGGTATGTGGAGCTTGCAGTTGGGGGCAACAAAGTTCATGCTGGCTGCAATGGGAAGCTCGTTTGGAGACACACGCCGTGGCAGGGTGCACATACTGCGAAAGGGCCTGTGAGACCCTTGCGTCGTGCTCTTCAGGGTCTTGATCCAAGATCTACAGCGAGTATGTTTGCTGACGCCAGATGTATTGGAGAAAGAAAGGTCAATGGGGAGGATTGCTTCATCCTCAAGCTGTGTGCTGATCCTCAAACTCTGAAGGCCAGGAGTGAAGGCCCTGCAGAGATAATTAGGCATGTGTTGTTTGGCTACTTCAGCCAGAAGACGGGGCTTCTTGTTCACATGGAGGACTCTCATTTGACCCGCATTCAATCCAATGGCGGTGATGCTGTTTACTGGGAAACCACAATCAATTCGTTCCTTGATGATTACAGACCTGTTGAGGGAATCATGATTGCGCACTCCGGCCGTTCTGTGGTTACCCTTTTCAGGTTTGGCGAGATGGCAATGAGCCATACCAAAACAAAGATGGAAGAAGCCTGGACAATTGAAGAGGTTGCATTTAATGTTCCAGGCTTGTCAATGGACTGCTTCATCCCCCCAGCTGACTTGAGATCTGAGACAATAATGAGTGAAGCATGCGAGCTTCCTCATGAAGAAAGGGGAAAGGGTGGCGGGATTGCAGCAGCAGTGCATCGGGCCAAAGTAGCTGCGCTGGAGAAAGAGCATGGTGCAAATGGTGATGGCATGAGCTGGAAAATGGAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

477

Amino Acids

52.22

Weight (kDa)

9.14

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 181 - 422 6.9e-122 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 789
Acc16I TGCGCA 1 cut(s) 1122
AccBSI CCGCTC 4 cut(s) 69, 75, 99, 391
AccII CGCG 3 cut(s) 51, 81, 297
AccIII TCCGGA 2 cut(s) 229, 661
AclWI GGATC 4 cut(s) 192, 240, 806, 902
AcoI YGGCCR 3 cut(s) 154, 160, 1129
AcuI CTGAAG 3 cut(s) 785, 941, 961
AcyI GRCGYC 1 cut(s) 843
AdeI CACNNNGTG 1 cut(s) 747
AfaI GTAC 2 cut(s) 476, 491
AflIII ACRYGT 1 cut(s) 666
AgsI TTSAA 3 cut(s) 581, 1034, 1214
AjiI CACGTC 1 cut(s) 406
AjnI CCWGG 3 cut(s) 926, 1202, 1234
AleI CACNNNNGTG 1 cut(s) 593
Alw21I GWGCWC 4 cut(s) 388, 648, 760, 799
Alw26I GTCTC 4 cut(s) 355, 730, 775, 1277
Alw44I GTGCAC 1 cut(s) 756
AlwI GGATC 4 cut(s) 192, 240, 806, 902
AlwNI CAGNNNCTG 1 cut(s) 517
Aor13HI TCCGGA 2 cut(s) 229, 661
ApaLI GTGCAC 1 cut(s) 756
ApeKI GCWGC 8 cut(s) 175, 204, 301, 517, 714, 1346, 1349, 1373
AspLEI GCGC 4 cut(s) 83, 297, 1123, 1378
AspS9I GGNCC 5 cut(s) 227, 283, 773, 938, 1362
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 5 cut(s) 334, 422, 607, 1058, 1415
AvaII GGWCC 1 cut(s) 227
AxyI CCTNAGG 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 760
BanII GRGCYC 2 cut(s) 186, 388
BbsI GAAGAC 2 cut(s) 608, 992
Bbv12I GWGCWC 4 cut(s) 388, 648, 760, 799
BbvCI CCTCAGC 2 cut(s) 56, 434
BbvI GCAGC 8 cut(s) 187, 216, 313, 504, 701, 1358, 1360, 1361
BccI CCATC 6 cut(s) 461, 541, 556, 1159, 1186, 1400
BceAI ACGGC 5 cut(s) 169, 175, 256, 730, 1116
BciT130I CCWGG 3 cut(s) 928, 1204, 1236
BcnI CCSGG 1 cut(s) 310
BcoDI GTCTC 4 cut(s) 355, 730, 775, 1277
BfaI CTAG 1 cut(s) 618
BfmI CTRYAG 3 cut(s) 518, 822, 942
BglII AGATCT 2 cut(s) 818, 1277
Bme1390I CCNGG 4 cut(s) 310, 928, 1204, 1236
Bme18I GGWCC 1 cut(s) 227
BmgBI CACGTC 1 cut(s) 406
BmgT120I GGNCC 5 cut(s) 227, 283, 773, 938, 1362
BmiI GGNNCC 2 cut(s) 183, 228
BmrFI CCNGG 4 cut(s) 310, 928, 1204, 1236
BmrI ACTGGG 2 cut(s) 466, 1069
BmsI GCATC 6 cut(s) 282, 447, 509, 623, 1039, 1366
BmuI ACTGGG 2 cut(s) 466, 1069
BpiI GAAGAC 2 cut(s) 608, 992
BplI GAGNNNNNCTC 2 cut(s) 375, 407
BpmI CTGGAG 1 cut(s) 1400
Bpu10I CCTNAGC 2 cut(s) 56, 434
BpuEI CTTGAG 2 cut(s) 878, 1294
BpuMI CCSGG 1 cut(s) 310
BsaHI GRCGYC 1 cut(s) 843
BsaI GGTCTC 1 cut(s) 775
BsaJI CCNNGG 4 cut(s) 151, 157, 591, 746
BsaWI WCCGGW 3 cut(s) 229, 345, 661
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 3 cut(s) 286, 472, 1064
Bse21I CCTNAGG 1 cut(s) 209
Bse3DI GCAATG 2 cut(s) 724, 1176
BseAI TCCGGA 2 cut(s) 229, 661
BseBI CCWGG 3 cut(s) 928, 1204, 1236
BseDI CCNNGG 4 cut(s) 151, 157, 591, 746
BseGI GGATG 4 cut(s) 297, 567, 888, 1257
BseMI GCAATG 2 cut(s) 724, 1176
BseMII CTCAG 6 cut(s) 70, 200, 350, 448, 564, 1272
BseNI ACTGG 3 cut(s) 286, 472, 1064
BseSI GKGCMC 1 cut(s) 760
BseX3I CGGCCG 1 cut(s) 1129
BseXI GCAGC 8 cut(s) 187, 216, 313, 504, 701, 1358, 1360, 1361
BseYI CCCAGC 1 cut(s) 1264
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 3 cut(s) 51, 81, 297
Bsh1285I CGRYCG 1 cut(s) 1132
BsiEI CGRYCG 1 cut(s) 1132
BsiHKAI GWGCWC 4 cut(s) 388, 648, 760, 799
BsiSI CCGG 8 cut(s) 179, 201, 215, 230, 310, 346, 662, 1128
BsmAI GTCTC 4 cut(s) 355, 730, 775, 1277
BsmI GAATGC 1 cut(s) 1029
Bso31I GGTCTC 1 cut(s) 775
Bsp1286I GDGCHC 5 cut(s) 186, 388, 648, 760, 799
Bsp13I TCCGGA 2 cut(s) 229, 661
Bsp143I GATC 8 cut(s) 189, 197, 232, 355, 811, 818, 907, 1277
BspCNI CTCAG 6 cut(s) 69, 201, 351, 447, 565, 1273
BspEI TCCGGA 2 cut(s) 229, 661
BspFNI CGCG 3 cut(s) 51, 81, 297
BspHI TCATGA 2 cut(s) 1113, 1315
BspLI GGNNCC 2 cut(s) 183, 228
BspMAI CTGCAG 2 cut(s) 522, 946
BspPI GGATC 4 cut(s) 192, 240, 806, 902
BspQI GCTCTTC 1 cut(s) 804
BspTNI GGTCTC 1 cut(s) 775
BsrBI CCGCTC 4 cut(s) 69, 75, 99, 391
BsrDI GCAATG 2 cut(s) 724, 1176
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 3 cut(s) 286, 472, 1064
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 4 cut(s) 151, 157, 591, 746
BssMI GATC 8 cut(s) 189, 197, 232, 355, 811, 818, 907, 1277
BssNI GRCGYC 1 cut(s) 843
BssT1I CCWWGG 1 cut(s) 591
Bst2UI CCWGG 3 cut(s) 928, 1204, 1236
Bst4CI ACNGT 1 cut(s) 489
Bst6I CTCTTC 2 cut(s) 804, 1209
BstACI GRCGYC 1 cut(s) 843
BstAPI GCANNNNNTGC 2 cut(s) 517, 764
BstC8I GCNNGC 8 cut(s) 97, 164, 299, 393, 684, 712, 1303, 1307
BstDEI CTNAG 6 cut(s) 56, 209, 359, 434, 573, 1281
BstDSI CCRYGG 3 cut(s) 151, 157, 746
BstEII GGTNACC 1 cut(s) 1141
BstF5I GGATG 4 cut(s) 297, 567, 888, 1257
BstFNI CGCG 3 cut(s) 51, 81, 297
BstHHI GCGC 4 cut(s) 83, 297, 1123, 1378
BstKTI GATC 8 cut(s) 192, 200, 235, 358, 814, 821, 910, 1280
BstMAI GTCTC 4 cut(s) 355, 730, 775, 1277
BstMBI GATC 8 cut(s) 189, 197, 232, 355, 811, 818, 907, 1277
BstMCI CGRYCG 1 cut(s) 1132
BstNI CCWGG 3 cut(s) 928, 1204, 1236
BstNSI RCATGY 3 cut(s) 670, 962, 1305
BstPI GGTNACC 1 cut(s) 1141
BstSCI CCNGG 4 cut(s) 308, 926, 1202, 1234
BstSFI CTRYAG 3 cut(s) 518, 822, 942
BstSLI GKGCMC 1 cut(s) 760
BstUI CGCG 3 cut(s) 51, 81, 297
BstV1I GCAGC 8 cut(s) 187, 216, 313, 504, 701, 1358, 1360, 1361
BstV2I GAAGAC 2 cut(s) 608, 992
BstX2I RGATCY 4 cut(s) 197, 232, 818, 1277
BstYI RGATCY 4 cut(s) 197, 232, 818, 1277
BstZI CGGCCG 1 cut(s) 1129
Bsu36I CCTNAGG 1 cut(s) 209
BtgI CCRYGG 3 cut(s) 151, 157, 746
BtgZI GCGATG 1 cut(s) 560
BtrI CACGTC 1 cut(s) 406
BtsCI GGATG 4 cut(s) 297, 567, 888, 1257
BtsI GCAGTG 1 cut(s) 1359
BtsIMutI CAGTG 2 cut(s) 293, 1359
Cac8I GCNNGC 8 cut(s) 97, 164, 299, 393, 684, 712, 1303, 1307
CaiI CAGNNNCTG 1 cut(s) 517
CciI TCATGA 2 cut(s) 1113, 1315
CfoI GCGC 4 cut(s) 83, 297, 1123, 1378
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 5 cut(s) 227, 283, 773, 938, 1362
CseI GACGC 2 cut(s) 779, 851
Csp6I GTAC 2 cut(s) 475, 490
CviQI GTAC 2 cut(s) 475, 490
DdeI CTNAG 6 cut(s) 56, 209, 359, 434, 573, 1281
DpnI GATC 8 cut(s) 191, 199, 234, 357, 813, 820, 909, 1279
DpnII GATC 8 cut(s) 189, 197, 232, 355, 811, 818, 907, 1277
DraIII CACNNNGTG 1 cut(s) 747
DrdI GACNNNNNNGTC 1 cut(s) 789
DseDI GACNNNNNNGTC 1 cut(s) 789
EaeI YGGCCR 3 cut(s) 154, 160, 1129
EagI CGGCCG 1 cut(s) 1129
Eam1104I CTCTTC 2 cut(s) 804, 1209
EarI CTCTTC 2 cut(s) 804, 1209
EciI GGCGGA 2 cut(s) 91, 116
Ecl136II GAGCTC 1 cut(s) 386
EclXI CGGCCG 1 cut(s) 1129
Eco130I CCWWGG 1 cut(s) 591
Eco24I GRGCYC 2 cut(s) 186, 388
Eco31I GGTCTC 1 cut(s) 775
Eco47I GGWCC 1 cut(s) 227
Eco52I CGGCCG 1 cut(s) 1129
Eco53kI GAGCTC 1 cut(s) 386
Eco57I CTGAAG 3 cut(s) 785, 941, 961
Eco81I CCTNAGG 1 cut(s) 209
Eco91I GGTNACC 1 cut(s) 1141
EcoICRI GAGCTC 1 cut(s) 386
EcoO109I RGGNCCY 2 cut(s) 773, 938
EcoO65I GGTNACC 1 cut(s) 1141
EcoRII CCWGG 3 cut(s) 926, 1202, 1234
EcoT14I CCWWGG 1 cut(s) 591
EcoT38I GRGCYC 2 cut(s) 186, 388
ErhI CCWWGG 1 cut(s) 591
FokI GGATG 4 cut(s) 304, 574, 875, 1244
FriOI GRGCYC 2 cut(s) 186, 388
FspBI CTAG 1 cut(s) 618
FspI TGCGCA 1 cut(s) 1122
GlaI GCGC 4 cut(s) 82, 296, 1122, 1377
GsaI CCCAGC 1 cut(s) 1268
GsuI CTGGAG 1 cut(s) 1400
HapII CCGG 8 cut(s) 179, 201, 215, 230, 310, 346, 662, 1128
HgaI GACGC 2 cut(s) 779, 851
HhaI GCGC 4 cut(s) 83, 297, 1123, 1378
Hin1I GRCGYC 1 cut(s) 843
Hin6I GCGC 4 cut(s) 81, 295, 1121, 1376
HinP1I GCGC 4 cut(s) 81, 295, 1121, 1376
HinfI GANTC 8 cut(s) 257, 313, 363, 375, 629, 658, 1013, 1110
HpaII CCGG 8 cut(s) 179, 201, 215, 230, 310, 346, 662, 1128
HphI GGTGA 5 cut(s) 334, 422, 607, 1058, 1415
Hpy166II GTNNAC 4 cut(s) 492, 758, 1003, 1057
Hpy188I TCNGA 7 cut(s) 355, 360, 374, 418, 574, 921, 1282
Hpy188III TCNNGA 8 cut(s) 193, 230, 236, 618, 662, 809, 1114, 1316
Hpy8I GTNNAC 4 cut(s) 492, 758, 1003, 1057
Hpy99I CGWCG 1 cut(s) 795
HpyAV CCTTC 3 cut(s) 327, 916, 929
HpyCH4III ACNGT 1 cut(s) 489
HpyCH4IV ACGT 1 cut(s) 405
HpyF3I CTNAG 6 cut(s) 56, 209, 359, 434, 573, 1281
HpySE526I ACGT 1 cut(s) 405
Hsp92I GRCGYC 1 cut(s) 843
HspAI GCGC 4 cut(s) 81, 295, 1121, 1376
Kpn2I TCCGGA 2 cut(s) 229, 661
Kzo9I GATC 8 cut(s) 189, 197, 232, 355, 811, 818, 907, 1277
LguI GCTCTTC 1 cut(s) 804
LmnI GCTCC 8 cut(s) 74, 80, 104, 129, 181, 383, 391, 679
Lsp1109I GCAGC 8 cut(s) 187, 216, 313, 504, 701, 1358, 1360, 1361
LweI GCATC 6 cut(s) 282, 447, 509, 623, 1039, 1366
MaeI CTAG 1 cut(s) 618
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 169, 1141
MalI GATC 8 cut(s) 191, 199, 234, 357, 813, 820, 909, 1279
MbiI CCGCTC 4 cut(s) 69, 75, 99, 391
MboI GATC 8 cut(s) 189, 197, 232, 355, 811, 818, 907, 1277
MboII GAAGA 8 cut(s) 13, 19, 613, 791, 997, 1208, 1226, 1331
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 4 cut(s) 197, 232, 818, 1277
MhlI GDGCHC 5 cut(s) 186, 388, 648, 760, 799
MluCI AATT 3 cut(s) 951, 1075, 1209
MlyI GAGTC 6 cut(s) 266, 307, 357, 384, 638, 1007
MmeI TCCRAC 1 cut(s) 441
MroI TCCGGA 2 cut(s) 229, 661
MseI TTAA 3 cut(s) 33, 531, 1227
MslI CAYNNNNRTG 2 cut(s) 593, 619
MspA1I CMGCKG 3 cut(s) 166, 497, 1268
MspI CCGG 8 cut(s) 179, 201, 215, 230, 310, 346, 662, 1128
MspR9I CCNGG 4 cut(s) 310, 928, 1204, 1236
MunI CAATTG 1 cut(s) 1209
Mva1269I GAATGC 1 cut(s) 1029
MvaI CCWGG 3 cut(s) 928, 1204, 1236
MvnI CGCG 3 cut(s) 51, 81, 297
NciI CCSGG 1 cut(s) 310
NdeII GATC 8 cut(s) 189, 197, 232, 355, 811, 818, 907, 1277
NlaIV GGNNCC 2 cut(s) 183, 228
NmuCI GTSAC 1 cut(s) 169
NsbI TGCGCA 1 cut(s) 1122
NspI RCATGY 3 cut(s) 670, 962, 1305
OliI CACNNNNGTG 1 cut(s) 593
PaeI GCATGC 1 cut(s) 1305
PagI TCATGA 2 cut(s) 1113, 1315
PciI ACATGT 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 804
PctI GAATGC 1 cut(s) 1029
PfeI GAWTC 2 cut(s) 658, 1110
PfoI TCCNGGA 1 cut(s) 308
PleI GAGTC 6 cut(s) 265, 307, 357, 383, 637, 1007
PpsI GAGTC 6 cut(s) 265, 307, 357, 383, 637, 1007
PscI ACATGT 1 cut(s) 666
Psp124BI GAGCTC 1 cut(s) 388
Psp6I CCWGG 3 cut(s) 926, 1202, 1234
PspEI GGTNACC 1 cut(s) 1141
PspFI CCCAGC 1 cut(s) 1264
PspGI CCWGG 3 cut(s) 926, 1202, 1234
PspN4I GGNNCC 2 cut(s) 183, 228
PspPI GGNCC 5 cut(s) 227, 283, 773, 938, 1362
PstI CTGCAG 2 cut(s) 522, 946
PstNI CAGNNNCTG 1 cut(s) 517
PsuI RGATCY 4 cut(s) 197, 232, 818, 1277
PvuII CAGCTG 2 cut(s) 166, 1268
RsaI GTAC 2 cut(s) 476, 491
RsaNI GTAC 2 cut(s) 475, 490
RseI CAYNNNNRTG 2 cut(s) 593, 619
SacI GAGCTC 1 cut(s) 388
SapI GCTCTTC 1 cut(s) 804
SaqAI TTAA 3 cut(s) 33, 531, 1227
Sau3AI GATC 8 cut(s) 189, 197, 232, 355, 811, 818, 907, 1277
Sau96I GGNCC 5 cut(s) 227, 283, 773, 938, 1362
SchI GAGTC 6 cut(s) 266, 307, 357, 384, 638, 1007
ScrFI CCNGG 4 cut(s) 310, 928, 1204, 1236
SduI GDGCHC 5 cut(s) 186, 388, 648, 760, 799
SfaNI GCATC 6 cut(s) 282, 447, 509, 623, 1039, 1366
SfcI CTRYAG 3 cut(s) 518, 822, 942
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 2 cut(s) 593, 619
SmlI CTYRAG 2 cut(s) 893, 1273
SmoI CTYRAG 2 cut(s) 893, 1273
SphI GCATGC 1 cut(s) 1305
Sse9I AATT 3 cut(s) 951, 1075, 1209
SspMI CTAG 1 cut(s) 618
SstI GAGCTC 1 cut(s) 388
StyD4I CCNGG 4 cut(s) 308, 926, 1202, 1234
StyI CCWWGG 1 cut(s) 591
TaaI ACNGT 1 cut(s) 489
TaiI ACGT 1 cut(s) 408
TaqI TCGA 3 cut(s) 260, 456, 527
TasI AATT 3 cut(s) 951, 1075, 1209
TatI WGTACW 2 cut(s) 474, 489
TauI GCSGC 3 cut(s) 54, 132, 500
TfiI GAWTC 2 cut(s) 658, 1110
Tru1I TTAA 3 cut(s) 33, 531, 1227
Tru9I TTAA 3 cut(s) 33, 531, 1227
TscAI CASTG 2 cut(s) 293, 1359
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 8 cut(s) 175, 204, 301, 517, 714, 1346, 1349, 1373
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 6 cut(s) 614, 671, 695, 877, 1246, 1332
TspRI CASTG 2 cut(s) 293, 1359
VneI GTGCAC 1 cut(s) 756
VpaK11BI GGWCC 1 cut(s) 227
XbaI TCTAGA 1 cut(s) 617
XceI RCATGY 3 cut(s) 670, 962, 1305
XcmI CCANNNNNNNNNTGG 1 cut(s) 1189
XspI CTAG 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.