pycom05g09310

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002346
Physical Location & Seq
Forward (+)
12482117 .. 12484069
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g09310.1

Sequence Viewer

Length: 960 bp
ATGGAGAAGAAACAAGGCTTCTTCTCTGCTCTCAAGGACGAGGTGGTTCGGGGTCTCAGCCCGTCGCGGTCGCGCGCCAGCACCCCGGCCCGGTCCGGGTCGCCCATGACGGGTCTGCTCCGGAGGAAGAAGCAGAACCATCACGGAAATGGGCACGGCGGCCAGCTGGTGGCCCAGCCGGACGCGTTGATTGGAAGATCCGGGAGTCTGAGGCCGGTGATGGAGGGTCCGGATCCGGACGGTGGGGAACTCGGGGATTCGAAGCGGGTCGGGTCGGGTTTGGGCCAGTGGATGCGAGGGCAACTCTCGCGGACTCCCTCCGTTAGCTCCGTCGCGCATAACAACAACAAGAGGTCTGATCTGAGGCTGCTGCTTGGAGTCATGGGCGCTCCTCTCGCCCCCCTCCACGTCAGCACCTCCGACCCTTTCCCCCATCTAAGCATCAAGGACACTCCCATCGAAGCTTCCTCTGCCCAGTATATACTGCAGCAGTACACAGCGGCTTCTGGTGGACAGAAACTGCAGAACTCGATCAAAAATGCTTATGCGATGGGAAAGGTTAGGATGGTAGCTTCTGAGTTTGAAACGGCTACTAAGGTGATGAAGACCAGAAATGCTTCTAGATGTGCCGAGTCGGGTGGGTTTGTTCTCTGGCAGATGAATCCGGACATGTGGTACGTGGAGCTTGCAGTGGGGGGAAGCAAGGTTCATGCTGGCTGCAATGGGAAGCTTGTCTGGAGGCACACGCCGTGGCTTGGTGCTCATACTGCGAAGGGACCCGTGAGACCCTTGCGTCGGGCACTTCAGGGTCTAGATCCAAGATCTACGGCTAGTATGTTTACCAATGCAAGATGTATAGGAGAGAGGAGGATCAATGGTGAGGATTGCTTCATCCTCAAGCTTTGTGCTGATCCTCAGACTCTGAAGGCCAGGAGTGAAGGCCCTGGAGATCGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

320

Amino Acids

34.4

Weight (kDa)

10.16

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 182 - 314 1.6e-59 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 792
AccB7I CCANNNNNTGG 1 cut(s) 169
AccII CGCG 6 cut(s) 67, 73, 75, 185, 310, 335
AccIII TCCGGA 4 cut(s) 120, 229, 235, 664
AciI CCGC 5 cut(s) 67, 159, 265, 310, 500
AclWI GGATC 6 cut(s) 192, 227, 240, 809, 878, 905
AcoI YGGCCR 1 cut(s) 160
AcuI CTGAAG 2 cut(s) 788, 944
AdeI CACNNNGTG 1 cut(s) 750
AfaI GTAC 2 cut(s) 494, 677
AfiI CCNNNNNNNGG 7 cut(s) 90, 96, 110, 169, 242, 755, 795
AflIII ACRYGT 2 cut(s) 183, 669
AgsI TTSAA 1 cut(s) 584
AjiI CACGTC 1 cut(s) 409
AjnI CCWGG 2 cut(s) 929, 943
AloI GAACNNNNNNTCC 2 cut(s) 690, 722
AluBI AGCT 7 cut(s) 166, 327, 464, 572, 685, 730, 901
AluI AGCT 7 cut(s) 166, 327, 464, 572, 685, 730, 901
Alw21I GWGCWC 1 cut(s) 763
Alw26I GTCTC 2 cut(s) 59, 778
AlwI GGATC 6 cut(s) 192, 227, 240, 809, 878, 905
AlwNI CAGNNNCTG 2 cut(s) 520, 922
Ama87I CYCGRG 1 cut(s) 251
Aor13HI TCCGGA 4 cut(s) 120, 229, 235, 664
AoxI GGCC 7 cut(s) 87, 160, 171, 212, 283, 927, 940
ApeKI GCWGC 4 cut(s) 367, 370, 487, 717
Asp700I GAANNNNTTC 1 cut(s) 616
AspLEI GCGC 4 cut(s) 75, 77, 337, 389
AspS9I GGNCC 7 cut(s) 88, 93, 172, 227, 283, 776, 941
AsuC2I CCSGG 4 cut(s) 86, 91, 97, 202
AsuHPI GGTGA 3 cut(s) 229, 610, 890
AsuII TTCGAA 1 cut(s) 260
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 3 cut(s) 93, 227, 776
BaeGI GKGCMC 2 cut(s) 156, 802
BamHI GGATCC 1 cut(s) 232
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 1 cut(s) 763
BbvI GCAGC 4 cut(s) 354, 357, 499, 704
BccI CCATC 6 cut(s) 147, 214, 441, 464, 544, 559
BceAI ACGGC 4 cut(s) 172, 603, 733, 843
BciT130I CCWGG 2 cut(s) 931, 945
BcnI CCSGG 4 cut(s) 86, 91, 97, 202
BcoDI GTCTC 2 cut(s) 59, 778
BfaI CTAG 3 cut(s) 621, 812, 831
BfmI CTRYAG 2 cut(s) 485, 521
BfoI RGCGCY 1 cut(s) 390
BglII AGATCT 1 cut(s) 821
BisI GCNGC 6 cut(s) 160, 368, 371, 488, 501, 718
BlsI GCNGC 6 cut(s) 161, 369, 372, 489, 502, 719
Bme1390I CCNGG 6 cut(s) 86, 91, 97, 202, 931, 945
Bme18I GGWCC 3 cut(s) 93, 227, 776
BmeT110I CYCGRG 1 cut(s) 251
BmgBI CACGTC 1 cut(s) 409
BmgT120I GGNCC 7 cut(s) 88, 93, 172, 227, 283, 776, 941
BmiI GGNNCC 4 cut(s) 228, 234, 777, 778
BmrFI CCNGG 6 cut(s) 86, 91, 97, 202, 931, 945
BmrI ACTGGG 1 cut(s) 469
BmsI GCATC 2 cut(s) 282, 450
BmuI ACTGGG 1 cut(s) 469
BpiI GAAGAC 1 cut(s) 611
BplI GAGNNNNNCTC 2 cut(s) 288, 320
BpmI CTGGAG 1 cut(s) 757
Bpu14I TTCGAA 1 cut(s) 260
BpuEI CTTGAG 2 cut(s) 17, 881
BpuMI CCSGG 4 cut(s) 86, 91, 97, 202
Bsa29I ATCGAT 1 cut(s) 952
BsaAI YACGTR 1 cut(s) 679
BsaI GGTCTC 2 cut(s) 59, 778
BsaJI CCNNGG 3 cut(s) 84, 749, 943
BsaWI WCCGGW 4 cut(s) 120, 229, 235, 664
Bsc4I CCNNNNNNNGG 7 cut(s) 90, 96, 110, 169, 242, 755, 795
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 286, 475
Bse3DI GCAATG 1 cut(s) 727
BseAI TCCGGA 4 cut(s) 120, 229, 235, 664
BseBI CCWGG 2 cut(s) 931, 945
BseCI ATCGAT 1 cut(s) 952
BseDI CCNNGG 3 cut(s) 84, 749, 943
BseGI GGATG 3 cut(s) 297, 570, 891
BseLI CCNNNNNNNGG 7 cut(s) 90, 96, 110, 169, 242, 755, 795
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 5 cut(s) 70, 200, 353, 567, 929
BseNI ACTGG 2 cut(s) 286, 475
BsePI GCGCGC 1 cut(s) 73
BseRI GAGGAG 2 cut(s) 381, 880
BseSI GKGCMC 2 cut(s) 156, 802
BseXI GCAGC 4 cut(s) 354, 357, 499, 704
BseYI CCCAGC 1 cut(s) 174
Bsh1236I CGCG 6 cut(s) 67, 73, 75, 185, 310, 335
Bsh1285I CGRYCG 1 cut(s) 71
BshFI GGCC 7 cut(s) 89, 162, 173, 214, 285, 929, 942
BshVI ATCGAT 1 cut(s) 952
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 763
BsiHKCI CYCGRG 1 cut(s) 251
BslFI GGGAC 1 cut(s) 789
BslI CCNNNNNNNGG 7 cut(s) 90, 96, 110, 169, 242, 755, 795
BsmAI GTCTC 2 cut(s) 59, 778
BsmFI GGGAC 1 cut(s) 789
BsnI GGCC 7 cut(s) 89, 162, 173, 214, 285, 929, 942
Bso31I GGTCTC 2 cut(s) 59, 778
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 260
Bsp1286I GDGCHC 3 cut(s) 156, 763, 802
Bsp13I TCCGGA 4 cut(s) 120, 229, 235, 664
BspACI CCGC 5 cut(s) 67, 159, 265, 310, 500
BspANI GGCC 7 cut(s) 89, 162, 173, 214, 285, 929, 942
BspCNI CTCAG 5 cut(s) 69, 201, 354, 568, 928
BspDI ATCGAT 1 cut(s) 952
BspEI TCCGGA 4 cut(s) 120, 229, 235, 664
BspFNI CGCG 6 cut(s) 67, 73, 75, 185, 310, 335
BspLI GGNNCC 4 cut(s) 228, 234, 777, 778
BspMAI CTGCAG 2 cut(s) 489, 525
BspPI GGATC 6 cut(s) 192, 227, 240, 809, 878, 905
BspT104I TTCGAA 1 cut(s) 260
BspTNI GGTCTC 2 cut(s) 59, 778
BsrDI GCAATG 1 cut(s) 727
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 2 cut(s) 286, 475
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 3 cut(s) 84, 749, 943
BssHII GCGCGC 1 cut(s) 73
Bst2UI CCWGG 2 cut(s) 931, 945
Bst4CI ACNGT 1 cut(s) 242
BstBAI YACGTR 1 cut(s) 679
BstBI TTCGAA 1 cut(s) 260
BstC8I GCNNGC 5 cut(s) 75, 79, 164, 687, 715
BstDEI CTNAG 7 cut(s) 56, 209, 362, 437, 576, 594, 915
BstDSI CCRYGG 1 cut(s) 749
BstF5I GGATG 3 cut(s) 297, 570, 891
BstFNI CGCG 6 cut(s) 67, 73, 75, 185, 310, 335
BstH2I RGCGCY 1 cut(s) 390
BstHHI GCGC 4 cut(s) 75, 77, 337, 389
BstMAI GTCTC 2 cut(s) 59, 778
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 4 cut(s) 307, 395, 470, 767
BstNI CCWGG 2 cut(s) 931, 945
BstNSI RCATGY 1 cut(s) 673
BstSCI CCNGG 6 cut(s) 84, 89, 95, 200, 929, 943
BstSFI CTRYAG 2 cut(s) 485, 521
BstSLI GKGCMC 2 cut(s) 156, 802
BstUI CGCG 6 cut(s) 67, 73, 75, 185, 310, 335
BstV1I GCAGC 4 cut(s) 354, 357, 499, 704
BstV2I GAAGAC 1 cut(s) 611
BstX2I RGATCY 4 cut(s) 197, 232, 814, 821
BstYI RGATCY 4 cut(s) 197, 232, 814, 821
Bsu15I ATCGAT 1 cut(s) 952
BsuRI GGCC 7 cut(s) 89, 162, 173, 214, 285, 929, 942
BsuTUI ATCGAT 1 cut(s) 952
BtgI CCRYGG 1 cut(s) 749
BtgZI GCGATG 1 cut(s) 563
BtrI CACGTC 1 cut(s) 409
BtsCI GGATG 3 cut(s) 297, 570, 891
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 2 cut(s) 293, 696
Cac8I GCNNGC 5 cut(s) 75, 79, 164, 687, 715
CaiI CAGNNNCTG 2 cut(s) 520, 922
CfoI GCGC 4 cut(s) 75, 77, 337, 389
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 7 cut(s) 88, 93, 172, 227, 283, 776, 941
ClaI ATCGAT 1 cut(s) 952
CpoI CGGWCCG 1 cut(s) 93
CseI GACGC 2 cut(s) 191, 782
Csp6I GTAC 2 cut(s) 493, 676
CspI CGGWCCG 1 cut(s) 93
CviAII CATG 4 cut(s) 106, 382, 670, 710
CviQI GTAC 2 cut(s) 493, 676
DdeI CTNAG 7 cut(s) 56, 209, 362, 437, 576, 594, 915
DraIII CACNNNGTG 1 cut(s) 750
DrdI GACNNNNNNGTC 1 cut(s) 792
DseDI GACNNNNNNGTC 1 cut(s) 792
EaeI YGGCCR 1 cut(s) 160
Eco31I GGTCTC 2 cut(s) 59, 778
Eco47I GGWCC 3 cut(s) 93, 227, 776
Eco57I CTGAAG 2 cut(s) 788, 944
Eco88I CYCGRG 1 cut(s) 251
EcoO109I RGGNCCY 2 cut(s) 776, 941
EcoRII CCWGG 2 cut(s) 929, 943
FaeI CATG 4 cut(s) 109, 385, 673, 713
FaqI GGGAC 1 cut(s) 789
FatI CATG 4 cut(s) 105, 381, 669, 709
FauI CCCGC 1 cut(s) 258
Fnu4HI GCNGC 6 cut(s) 160, 368, 371, 488, 501, 718
FokI GGATG 3 cut(s) 304, 577, 878
Fsp4HI GCNGC 6 cut(s) 160, 368, 371, 488, 501, 718
FspBI CTAG 3 cut(s) 621, 812, 831
GlaI GCGC 4 cut(s) 74, 76, 336, 388
GluI GCNGC 6 cut(s) 160, 368, 371, 488, 501, 718
GsaI CCCAGC 1 cut(s) 178
GsuI CTGGAG 1 cut(s) 757
HaeII RGCGCY 1 cut(s) 390
HaeIII GGCC 7 cut(s) 89, 162, 173, 214, 285, 929, 942
HgaI GACGC 2 cut(s) 191, 782
HhaI GCGC 4 cut(s) 75, 77, 337, 389
Hin1II CATG 4 cut(s) 109, 385, 673, 713
Hin6I GCGC 4 cut(s) 73, 75, 335, 387
HinP1I GCGC 4 cut(s) 73, 75, 335, 387
HindIII AAGCTT 3 cut(s) 462, 728, 899
HinfI GANTC 7 cut(s) 205, 257, 313, 378, 632, 661, 919
HphI GGTGA 3 cut(s) 229, 610, 890
Hpy166II GTNNAC 3 cut(s) 495, 512, 840
Hpy188I TCNGA 7 cut(s) 210, 358, 363, 421, 577, 918, 924
Hpy188III TCNNGA 7 cut(s) 121, 230, 236, 621, 665, 736, 812
Hpy8I GTNNAC 3 cut(s) 495, 512, 840
Hpy99I CGWCG 3 cut(s) 67, 335, 798
HpyAV CCTTC 3 cut(s) 766, 919, 932
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 2 cut(s) 408, 678
HpyCH4V TGCA 5 cut(s) 487, 523, 689, 720, 848
HpyF10VI GCNNNNNNNGC 4 cut(s) 307, 395, 470, 767
HpyF3I CTNAG 7 cut(s) 56, 209, 362, 437, 576, 594, 915
HpySE526I ACGT 2 cut(s) 408, 678
Hsp92II CATG 4 cut(s) 109, 385, 673, 713
HspAI GCGC 4 cut(s) 73, 75, 335, 387
KflI GGGWCCC 1 cut(s) 776
Kpn2I TCCGGA 4 cut(s) 120, 229, 235, 664
LmnI GCTCC 4 cut(s) 123, 332, 394, 682
Lsp1109I GCAGC 4 cut(s) 354, 357, 499, 704
LweI GCATC 2 cut(s) 282, 450
MaeI CTAG 3 cut(s) 621, 812, 831
MaeII ACGT 2 cut(s) 408, 678
MboII GAAGA 5 cut(s) 13, 19, 139, 207, 616
MflI RGATCY 4 cut(s) 197, 232, 814, 821
MhlI GDGCHC 3 cut(s) 156, 763, 802
MluI ACGCGT 1 cut(s) 183
MlyI GAGTC 5 cut(s) 214, 307, 387, 641, 913
MmeI TCCRAC 1 cut(s) 444
MroI TCCGGA 4 cut(s) 120, 229, 235, 664
MroXI GAANNNNTTC 1 cut(s) 616
MslI CAYNNNNRTG 1 cut(s) 147
MspA1I CMGCKG 2 cut(s) 166, 500
MspR9I CCNGG 6 cut(s) 86, 91, 97, 202, 931, 945
MvaI CCWGG 2 cut(s) 931, 945
MvnI CGCG 6 cut(s) 67, 73, 75, 185, 310, 335
MwoI GCNNNNNNNGC 4 cut(s) 307, 395, 470, 767
NciI CCSGG 4 cut(s) 86, 91, 97, 202
NlaIII CATG 4 cut(s) 109, 385, 673, 713
NlaIV GGNNCC 4 cut(s) 228, 234, 777, 778
NmeAIII GCCGAG 1 cut(s) 655
NspI RCATGY 1 cut(s) 673
NspV TTCGAA 1 cut(s) 260
PauI GCGCGC 1 cut(s) 73
PciI ACATGT 1 cut(s) 669
PdmI GAANNNNTTC 1 cut(s) 616
PfeI GAWTC 2 cut(s) 257, 661
PflMI CCANNNNNTGG 1 cut(s) 169
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 6 cut(s) 161, 369, 372, 489, 502, 719
PleI GAGTC 5 cut(s) 213, 307, 386, 640, 913
PpsI GAGTC 5 cut(s) 213, 307, 386, 640, 913
Ppu21I YACGTR 1 cut(s) 679
PpuMI RGGWCCY 1 cut(s) 776
PscI ACATGT 1 cut(s) 669
Psp5II RGGWCCY 1 cut(s) 776
Psp6I CCWGG 2 cut(s) 929, 943
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 2 cut(s) 929, 943
PspN4I GGNNCC 4 cut(s) 228, 234, 777, 778
PspPI GGNCC 7 cut(s) 88, 93, 172, 227, 283, 776, 941
PspPPI RGGWCCY 1 cut(s) 776
PstI CTGCAG 2 cut(s) 489, 525
PstNI CAGNNNCTG 2 cut(s) 520, 922
PsuI RGATCY 4 cut(s) 197, 232, 814, 821
PteI GCGCGC 1 cut(s) 73
PvuII CAGCTG 1 cut(s) 166
RsaI GTAC 2 cut(s) 494, 677
RsaNI GTAC 2 cut(s) 493, 676
RseI CAYNNNNRTG 1 cut(s) 147
Rsr2I CGGWCCG 1 cut(s) 93
RsrII CGGWCCG 1 cut(s) 93
SatI GCNGC 6 cut(s) 160, 368, 371, 488, 501, 718
Sau96I GGNCC 7 cut(s) 88, 93, 172, 227, 283, 776, 941
SchI GAGTC 5 cut(s) 214, 307, 387, 641, 913
ScrFI CCNGG 6 cut(s) 86, 91, 97, 202, 931, 945
SduI GDGCHC 3 cut(s) 156, 763, 802
SfaNI GCATC 2 cut(s) 282, 450
SfcI CTRYAG 2 cut(s) 485, 521
SfuI TTCGAA 1 cut(s) 260
SinI GGWCC 3 cut(s) 93, 227, 776
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 2 cut(s) 32, 896
SmoI CTYRAG 2 cut(s) 32, 896
SsiI CCGC 5 cut(s) 67, 159, 265, 310, 500
SspMI CTAG 3 cut(s) 621, 812, 831
StyD4I CCNGG 6 cut(s) 84, 89, 95, 200, 929, 943
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 2 cut(s) 411, 681
TaqI TCGA 4 cut(s) 260, 459, 530, 952
TatI WGTACW 1 cut(s) 492
TauI GCSGC 2 cut(s) 162, 503
TfiI GAWTC 2 cut(s) 257, 661
TscAI CASTG 2 cut(s) 293, 696
TseI GCWGC 4 cut(s) 367, 370, 487, 717
TspDTI ATGAA 4 cut(s) 617, 674, 698, 880
TspGWI ACGGA 3 cut(s) 159, 310, 319
TspRI CASTG 2 cut(s) 293, 696
Van91I CCANNNNNTGG 1 cut(s) 169
VpaK11BI GGWCC 3 cut(s) 93, 227, 776
XbaI TCTAGA 2 cut(s) 620, 811
XceI RCATGY 1 cut(s) 673
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 616
XspI CTAG 3 cut(s) 621, 812, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.