MD10G1100800.v1.1

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002346
Physical Location & Seq
Forward (+)
16354739 .. 16358168
3430 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1100800.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGGAGAAGAAACAAGGCTTCTTCTCTGCTCTCAAGGACGAGGTGGTTCGGGGTCTCAGCCCCTCGCGGTCGCGGGCCAGCAGCCCGGCCCGGTCCGGGTCGCCCATGACGGGTCTGCTCCGGAGGAAGAAGCAGAACCATCACGGAAATGGGCACGGCGTCCAGCTGGTGGCGCAGCCGGACGCGTTGATTGGGAGATCCGGGAGCCTGAGGCCGGTGATGGAGGGTCCGGATCCGGACGGAGGGGAACTTGGGGATTCGAAGCGGGTCGGGTCGAGTTTGGGCCAGTGGATGCGAGGGCAGCTGTCGCGGACTCCCTCCGTGAGCTCCGTCGCGCATAACAACAGCAAGAGTTCTGATCTGAGACTGCTGCTTGGAGTCATGGGTGCTCCTCTCGCCCCCCTCCACGTCAGTACCTCCGACCCTTTCCCCCATCTCAGCATCAAGGACACTCCCATCGAAACTTCCTCTGCCCAGTACATACTGCAACAGTACACAGCGGCATCTGGTGGGCAGAAGCTGCAGAACTCGATAAAAAATGCTTATGCCATGGGAAAGGTTAGGATGGTTGCTTCTGAGTTTGAAACGGCAACGAAGGTGATGAAGACCAGAAATGCCTCTAGATGTGCCGAGTCAGGTGGGTTTGTGCTCTGGCAGATGAGTCCGGACATGTGGTATGTAGAGCTTGCGGTGGGTGGGAGCAAGGTCCATGCCGGCTGCAATGGGAAGCTTGTCTGGAGGCACACACCGTGGCTTGGTGCTCATACTGCGAAAGGACCAGTGAGACCCTTGCGTCGGGCACTTCAGGGTCTAGATCCAAGATCTACGGCTAGTATGTTTACCGATGCAAGATGTATAGGAGAGAGGAAGATCAATGGTGAGGATTGCTTCATCCTCAAGCTTTCTGCTGATCCTCAGACTCTGAAGGCCAGGAGTGAAGGCCCTGCCGAGATCATAAGGCATGTGTTGTTTGGTTACTTCAGCCAGAAAACAGGGCTTCTTGTTCATATGGAGGATTCACATCTGACCCGCATCCAATCCAACGGTGGCGATGCTGTTTACTGGGAAACCACAATCAATTCATTCCTTGATGATTACAGGCCTGTTGAAGGAATCATGATTGCACACTCAGGGCGTTCCGTGGTAACCCTATTCAGGTTTGGTGAAATGGCAATGAGCCATACCAAAACAAGGATGGAAGAAGCTTGGACAATCGAGGAGGTTGCATTTAATGTTCCAGGTTTGTCAATGGATTGCTTCATCCCCCCAGCTGACTTAAGATCGGGGACAATCAGTGAAGCGAGAGAGCTTCCACATGATGAAAGGGGAAAGGGTGGTGGGATTGCAGTAGCAACACATCGGGCCAAAGTTACTGCGCTGGAGAAAGAGCACAATGCTAATGTTGATAGCCTGACCTGGAAGATGGAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

478

Amino Acids

52.01

Weight (kDa)

9.32

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 182 - 423 1.8e-121 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 792
AccB7I CCANNNNNTGG 1 cut(s) 169
AccII CGCG 5 cut(s) 67, 73, 185, 310, 335
AccIII TCCGGA 4 cut(s) 120, 229, 235, 664
AciI CCGC 7 cut(s) 67, 73, 265, 310, 500, 689, 1030
AclWI GGATC 5 cut(s) 192, 227, 240, 809, 905
AcuI CTGAAG 3 cut(s) 788, 944, 964
AcyI GRCGYC 1 cut(s) 159
AdeI CACNNNGTG 1 cut(s) 750
AfaI GTAC 3 cut(s) 415, 479, 494
AflII CTTAAG 1 cut(s) 1276
AflIII ACRYGT 2 cut(s) 183, 669
AgsI TTSAA 2 cut(s) 584, 1109
AjiI CACGTC 1 cut(s) 409
AjnI CCWGG 3 cut(s) 929, 1237, 1415
Alw21I GWGCWC 5 cut(s) 329, 391, 651, 763, 1392
Alw26I GTCTC 3 cut(s) 59, 358, 778
AlwI GGATC 5 cut(s) 192, 227, 240, 809, 905
AlwNI CAGNNNCTG 2 cut(s) 520, 922
Aor13HI TCCGGA 4 cut(s) 120, 229, 235, 664
AoxI GGCC 8 cut(s) 75, 87, 212, 283, 927, 940, 1100, 1362
ApeKI GCWGC 6 cut(s) 81, 175, 301, 370, 520, 717
AspLEI GCGC 3 cut(s) 175, 337, 1378
AspS9I GGNCC 9 cut(s) 75, 88, 93, 227, 283, 706, 776, 941, 1362
AsuC2I CCSGG 4 cut(s) 86, 91, 97, 202
AsuHPI GGTGA 4 cut(s) 229, 610, 890, 1175
AsuII TTCGAA 1 cut(s) 260
AvaII GGWCC 4 cut(s) 93, 227, 706, 776
AxyI CCTNAGG 1 cut(s) 209
BaeGI GKGCMC 2 cut(s) 156, 802
BaeI ACNNNNGTAYC 2 cut(s) 397, 430
BamHI GGATCC 1 cut(s) 232
BanII GRGCYC 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 5 cut(s) 329, 391, 651, 763, 1392
BbvI GCAGC 6 cut(s) 93, 187, 313, 357, 507, 704
BccI CCATC 7 cut(s) 147, 214, 441, 464, 559, 1189, 1417
BceAI ACGGC 3 cut(s) 172, 603, 843
BcgI CGANNNNNNTGC 2 cut(s) 1205, 1239
BciT130I CCWGG 3 cut(s) 931, 1239, 1417
BcnI CCSGG 4 cut(s) 86, 91, 97, 202
BcoDI GTCTC 3 cut(s) 59, 358, 778
BfaI CTAG 3 cut(s) 621, 812, 831
BfmI CTRYAG 1 cut(s) 521
BfrI CTTAAG 1 cut(s) 1276
BglII AGATCT 1 cut(s) 821
BisI GCNGC 7 cut(s) 82, 176, 302, 371, 501, 521, 718
BlsI GCNGC 7 cut(s) 83, 177, 303, 372, 502, 522, 719
Bme1390I CCNGG 7 cut(s) 86, 91, 97, 202, 931, 1239, 1417
Bme18I GGWCC 4 cut(s) 93, 227, 706, 776
BmgBI CACGTC 1 cut(s) 409
BmgT120I GGNCC 9 cut(s) 75, 88, 93, 227, 283, 706, 776, 941, 1362
BmiI GGNNCC 3 cut(s) 206, 228, 234
BmrFI CCNGG 7 cut(s) 86, 91, 97, 202, 931, 1239, 1417
BmrI ACTGGG 2 cut(s) 469, 1072
BmsI GCATC 6 cut(s) 282, 450, 512, 835, 1041, 1042
BmuI ACTGGG 2 cut(s) 469, 1072
BpiI GAAGAC 1 cut(s) 611
BpmI CTGGAG 2 cut(s) 757, 1400
Bpu14I TTCGAA 1 cut(s) 260
BpuEI CTTGAG 2 cut(s) 17, 881
BpuMI CCSGG 4 cut(s) 86, 91, 97, 202
BsaBI GATNNNNATC 1 cut(s) 1020
BsaHI GRCGYC 1 cut(s) 159
BsaI GGTCTC 2 cut(s) 59, 778
BsaJI CCNNGG 3 cut(s) 549, 749, 1140
BsaWI WCCGGW 4 cut(s) 120, 229, 235, 664
Bse118I RCCGGY 2 cut(s) 214, 713
Bse1I ACTGG 4 cut(s) 286, 475, 779, 1067
Bse21I CCTNAGG 1 cut(s) 209
Bse3DI GCAATG 2 cut(s) 727, 1179
Bse8I GATNNNNATC 1 cut(s) 1020
BseAI TCCGGA 4 cut(s) 120, 229, 235, 664
BseBI CCWGG 3 cut(s) 931, 1239, 1417
BseDI CCNNGG 3 cut(s) 549, 749, 1140
BseGI GGATG 6 cut(s) 297, 570, 891, 1032, 1200, 1260
BseJI GATNNNNATC 1 cut(s) 1020
BseMI GCAATG 2 cut(s) 727, 1179
BseMII CTCAG 7 cut(s) 70, 200, 353, 451, 567, 929, 1143
BseNI ACTGG 4 cut(s) 286, 475, 779, 1067
BseRI GAGGAG 2 cut(s) 381, 1232
BseSI GKGCMC 2 cut(s) 156, 802
BseXI GCAGC 6 cut(s) 93, 187, 313, 357, 507, 704
BseYI CCCAGC 1 cut(s) 1267
Bsh1236I CGCG 5 cut(s) 67, 73, 185, 310, 335
Bsh1285I CGRYCG 1 cut(s) 71
BshFI GGCC 8 cut(s) 77, 89, 214, 285, 929, 942, 1102, 1364
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 5 cut(s) 329, 391, 651, 763, 1392
BslFI GGGAC 1 cut(s) 1300
BsmAI GTCTC 3 cut(s) 59, 358, 778
BsmFI GGGAC 1 cut(s) 1300
BsnI GGCC 8 cut(s) 77, 89, 214, 285, 929, 942, 1102, 1364
Bso31I GGTCTC 2 cut(s) 59, 778
Bsp119I TTCGAA 1 cut(s) 260
Bsp1286I GDGCHC 7 cut(s) 156, 329, 391, 651, 763, 802, 1392
Bsp13I TCCGGA 4 cut(s) 120, 229, 235, 664
Bsp143I GATC 9 cut(s) 197, 232, 358, 814, 821, 870, 910, 951, 1280
Bsp19I CCATGG 1 cut(s) 549
BspACI CCGC 7 cut(s) 67, 73, 265, 310, 500, 689, 1030
BspANI GGCC 8 cut(s) 77, 89, 214, 285, 929, 942, 1102, 1364
BspCNI CTCAG 7 cut(s) 69, 201, 354, 450, 568, 928, 1142
BspEI TCCGGA 4 cut(s) 120, 229, 235, 664
BspFNI CGCG 5 cut(s) 67, 73, 185, 310, 335
BspHI TCATGA 1 cut(s) 1116
BspLI GGNNCC 3 cut(s) 206, 228, 234
BspMAI CTGCAG 1 cut(s) 525
BspPI GGATC 5 cut(s) 192, 227, 240, 809, 905
BspT104I TTCGAA 1 cut(s) 260
BspTI CTTAAG 1 cut(s) 1276
BspTNI GGTCTC 2 cut(s) 59, 778
BsrDI GCAATG 2 cut(s) 727, 1179
BsrFI RCCGGY 2 cut(s) 214, 713
BsrI ACTGG 4 cut(s) 286, 475, 779, 1067
BssAI RCCGGY 2 cut(s) 214, 713
BssECI CCNNGG 3 cut(s) 549, 749, 1140
BssMI GATC 9 cut(s) 197, 232, 358, 814, 821, 870, 910, 951, 1280
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 549
Bst2UI CCWGG 3 cut(s) 931, 1239, 1417
Bst4CI ACNGT 3 cut(s) 492, 750, 1046
BstACI GRCGYC 1 cut(s) 159
BstAFI CTTAAG 1 cut(s) 1276
BstAPI GCANNNNNTGC 1 cut(s) 520
BstBI TTCGAA 1 cut(s) 260
BstC8I GCNNGC 4 cut(s) 75, 79, 687, 715
BstDEI CTNAG 7 cut(s) 56, 209, 362, 437, 576, 915, 1129
BstDSI CCRYGG 3 cut(s) 549, 749, 1140
BstEII GGTNACC 1 cut(s) 1144
BstENI CCTNNNNNAGG 1 cut(s) 1107
BstF5I GGATG 6 cut(s) 297, 570, 891, 1032, 1200, 1260
BstFNI CGCG 5 cut(s) 67, 73, 185, 310, 335
BstHHI GCGC 3 cut(s) 175, 337, 1378
BstKTI GATC 9 cut(s) 200, 235, 361, 817, 824, 873, 913, 954, 1283
BstMAI GTCTC 3 cut(s) 59, 358, 778
BstMBI GATC 9 cut(s) 197, 232, 358, 814, 821, 870, 910, 951, 1280
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 6 cut(s) 172, 301, 307, 395, 520, 767
BstNI CCWGG 3 cut(s) 931, 1239, 1417
BstNSI RCATGY 2 cut(s) 673, 965
BstPI GGTNACC 1 cut(s) 1144
BstSCI CCNGG 7 cut(s) 84, 89, 95, 200, 929, 1237, 1415
BstSFI CTRYAG 1 cut(s) 521
BstSLI GKGCMC 2 cut(s) 156, 802
BstUI CGCG 5 cut(s) 67, 73, 185, 310, 335
BstV1I GCAGC 6 cut(s) 93, 187, 313, 357, 507, 704
BstV2I GAAGAC 1 cut(s) 611
BstX2I RGATCY 4 cut(s) 197, 232, 814, 821
BstYI RGATCY 4 cut(s) 197, 232, 814, 821
Bsu36I CCTNAGG 1 cut(s) 209
BsuRI GGCC 8 cut(s) 77, 89, 214, 285, 929, 942, 1102, 1364
BtgI CCRYGG 3 cut(s) 549, 749, 1140
BtgZI GCGATG 1 cut(s) 1065
BtrI CACGTC 1 cut(s) 409
BtsCI GGATG 6 cut(s) 297, 570, 891, 1032, 1200, 1260
BtsIMutI CAGTG 3 cut(s) 293, 786, 1300
Cac8I GCNNGC 4 cut(s) 75, 79, 687, 715
CaiI CAGNNNCTG 2 cut(s) 520, 922
CciI TCATGA 1 cut(s) 1116
CfoI GCGC 3 cut(s) 175, 337, 1378
Cfr10I RCCGGY 2 cut(s) 214, 713
Cfr13I GGNCC 9 cut(s) 75, 88, 93, 227, 283, 706, 776, 941, 1362
CpoI CGGWCCG 1 cut(s) 93
CseI GACGC 3 cut(s) 148, 191, 782
Csp6I GTAC 3 cut(s) 414, 478, 493
CspI CGGWCCG 1 cut(s) 93
CviAII CATG 8 cut(s) 106, 382, 550, 670, 710, 962, 1117, 1316
CviQI GTAC 3 cut(s) 414, 478, 493
DdeI CTNAG 7 cut(s) 56, 209, 362, 437, 576, 915, 1129
DpnI GATC 9 cut(s) 199, 234, 360, 816, 823, 872, 912, 953, 1282
DpnII GATC 9 cut(s) 197, 232, 358, 814, 821, 870, 910, 951, 1280
DraIII CACNNNGTG 1 cut(s) 750
DrdI GACNNNNNNGTC 1 cut(s) 792
DseDI GACNNNNNNGTC 1 cut(s) 792
Ecl136II GAGCTC 1 cut(s) 327
Eco130I CCWWGG 1 cut(s) 549
Eco147I AGGCCT 1 cut(s) 1102
Eco24I GRGCYC 1 cut(s) 329
Eco31I GGTCTC 2 cut(s) 59, 778
Eco47I GGWCC 4 cut(s) 93, 227, 706, 776
Eco53kI GAGCTC 1 cut(s) 327
Eco57I CTGAAG 3 cut(s) 788, 944, 964
Eco81I CCTNAGG 1 cut(s) 209
Eco91I GGTNACC 1 cut(s) 1144
EcoICRI GAGCTC 1 cut(s) 327
EcoNI CCTNNNNNAGG 1 cut(s) 1107
EcoO109I RGGNCCY 1 cut(s) 941
EcoO65I GGTNACC 1 cut(s) 1144
EcoRII CCWGG 3 cut(s) 929, 1237, 1415
EcoT14I CCWWGG 1 cut(s) 549
EcoT38I GRGCYC 1 cut(s) 329
ErhI CCWWGG 1 cut(s) 549
FaeI CATG 8 cut(s) 109, 385, 553, 673, 713, 965, 1120, 1319
FaqI GGGAC 1 cut(s) 1300
FatI CATG 8 cut(s) 105, 381, 549, 669, 709, 961, 1116, 1315
FauI CCCGC 3 cut(s) 66, 258, 1037
FauNDI CATATG 1 cut(s) 1008
Fnu4HI GCNGC 7 cut(s) 82, 176, 302, 371, 501, 521, 718
FokI GGATG 6 cut(s) 304, 577, 878, 1019, 1207, 1247
FriOI GRGCYC 1 cut(s) 329
Fsp4HI GCNGC 7 cut(s) 82, 176, 302, 371, 501, 521, 718
FspBI CTAG 3 cut(s) 621, 812, 831
GlaI GCGC 3 cut(s) 174, 336, 1377
GluI GCNGC 7 cut(s) 82, 176, 302, 371, 501, 521, 718
GsaI CCCAGC 1 cut(s) 1271
GsuI CTGGAG 2 cut(s) 757, 1400
HaeIII GGCC 8 cut(s) 77, 89, 214, 285, 929, 942, 1102, 1364
HgaI GACGC 3 cut(s) 148, 191, 782
HhaI GCGC 3 cut(s) 175, 337, 1378
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 8 cut(s) 109, 385, 553, 673, 713, 965, 1120, 1319
Hin6I GCGC 3 cut(s) 173, 335, 1376
HinP1I GCGC 3 cut(s) 173, 335, 1376
HindIII AAGCTT 3 cut(s) 728, 899, 1203
HinfI GANTC 8 cut(s) 257, 313, 378, 632, 661, 919, 1016, 1113
HphI GGTGA 4 cut(s) 229, 610, 890, 1175
Hpy166II GTNNAC 3 cut(s) 495, 840, 1060
Hpy188I TCNGA 7 cut(s) 358, 363, 421, 577, 918, 924, 1026
Hpy188III TCNNGA 8 cut(s) 121, 230, 236, 621, 665, 736, 812, 1117
Hpy8I GTNNAC 3 cut(s) 495, 840, 1060
Hpy99I CGWCG 2 cut(s) 335, 798
HpyAV CCTTC 4 cut(s) 589, 919, 932, 1103
HpyCH4III ACNGT 3 cut(s) 492, 750, 1046
HpyCH4IV ACGT 1 cut(s) 408
HpyCH4V TGCA 7 cut(s) 487, 523, 720, 848, 1124, 1226, 1346
HpyF10VI GCNNNNNNNGC 6 cut(s) 172, 301, 307, 395, 520, 767
HpyF3I CTNAG 7 cut(s) 56, 209, 362, 437, 576, 915, 1129
HpySE526I ACGT 1 cut(s) 408
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 8 cut(s) 109, 385, 553, 673, 713, 965, 1120, 1319
HspAI GCGC 3 cut(s) 173, 335, 1376
Kpn2I TCCGGA 4 cut(s) 120, 229, 235, 664
KroI GCCGGC 1 cut(s) 713
KroNI GCCGGC 1 cut(s) 715
Kzo9I GATC 9 cut(s) 197, 232, 358, 814, 821, 870, 910, 951, 1280
LmnI GCTCC 5 cut(s) 123, 204, 332, 394, 699
Lsp1109I GCAGC 6 cut(s) 93, 187, 313, 357, 507, 704
LweI GCATC 6 cut(s) 282, 450, 512, 835, 1041, 1042
MaeI CTAG 3 cut(s) 621, 812, 831
MaeII ACGT 1 cut(s) 408
MaeIII GTNAC 3 cut(s) 974, 1144, 1369
MalI GATC 9 cut(s) 199, 234, 360, 816, 823, 872, 912, 953, 1282
MboI GATC 9 cut(s) 197, 232, 358, 814, 821, 870, 910, 951, 1280
MboII GAAGA 7 cut(s) 13, 19, 139, 616, 880, 1211, 1432
MflI RGATCY 4 cut(s) 197, 232, 814, 821
MhlI GDGCHC 7 cut(s) 156, 329, 391, 651, 763, 802, 1392
MluCI AATT 1 cut(s) 1078
MluI ACGCGT 1 cut(s) 183
MlyI GAGTC 5 cut(s) 307, 387, 641, 670, 913
MmeI TCCRAC 2 cut(s) 444, 1065
MroI TCCGGA 4 cut(s) 120, 229, 235, 664
MroNI GCCGGC 1 cut(s) 713
MseI TTAA 2 cut(s) 1230, 1277
MslI CAYNNNNRTG 1 cut(s) 147
MspA1I CMGCKG 4 cut(s) 166, 304, 500, 1271
MspCI CTTAAG 1 cut(s) 1276
MspR9I CCNGG 7 cut(s) 86, 91, 97, 202, 931, 1239, 1417
MvaI CCWGG 3 cut(s) 931, 1239, 1417
MvnI CGCG 5 cut(s) 67, 73, 185, 310, 335
MwoI GCNNNNNNNGC 6 cut(s) 172, 301, 307, 395, 520, 767
NaeI GCCGGC 1 cut(s) 715
NciI CCSGG 4 cut(s) 86, 91, 97, 202
NcoI CCATGG 1 cut(s) 549
NdeI CATATG 1 cut(s) 1008
NdeII GATC 9 cut(s) 197, 232, 358, 814, 821, 870, 910, 951, 1280
NgoMIV GCCGGC 1 cut(s) 713
NlaIII CATG 8 cut(s) 109, 385, 553, 673, 713, 965, 1120, 1319
NlaIV GGNNCC 3 cut(s) 206, 228, 234
NmeAIII GCCGAG 2 cut(s) 655, 973
NspI RCATGY 2 cut(s) 673, 965
NspV TTCGAA 1 cut(s) 260
PagI TCATGA 1 cut(s) 1116
PceI AGGCCT 1 cut(s) 1102
PciI ACATGT 1 cut(s) 669
PdiI GCCGGC 1 cut(s) 715
PfeI GAWTC 3 cut(s) 257, 1016, 1113
PflMI CCANNNNNTGG 1 cut(s) 169
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 7 cut(s) 83, 177, 303, 372, 502, 522, 719
PleI GAGTC 5 cut(s) 307, 386, 640, 669, 913
PpsI GAGTC 5 cut(s) 307, 386, 640, 669, 913
PscI ACATGT 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 329
Psp6I CCWGG 3 cut(s) 929, 1237, 1415
PspEI GGTNACC 1 cut(s) 1144
PspFI CCCAGC 1 cut(s) 1267
PspGI CCWGG 3 cut(s) 929, 1237, 1415
PspN4I GGNNCC 3 cut(s) 206, 228, 234
PspPI GGNCC 9 cut(s) 75, 88, 93, 227, 283, 706, 776, 941, 1362
PstI CTGCAG 1 cut(s) 525
PstNI CAGNNNCTG 2 cut(s) 520, 922
PsuI RGATCY 4 cut(s) 197, 232, 814, 821
PvuII CAGCTG 3 cut(s) 166, 304, 1271
RsaI GTAC 3 cut(s) 415, 479, 494
RsaNI GTAC 3 cut(s) 414, 478, 493
RseI CAYNNNNRTG 1 cut(s) 147
Rsr2I CGGWCCG 1 cut(s) 93
RsrII CGGWCCG 1 cut(s) 93
SacI GAGCTC 1 cut(s) 329
SaqAI TTAA 2 cut(s) 1230, 1277
SatI GCNGC 7 cut(s) 82, 176, 302, 371, 501, 521, 718
Sau3AI GATC 9 cut(s) 197, 232, 358, 814, 821, 870, 910, 951, 1280
Sau96I GGNCC 9 cut(s) 75, 88, 93, 227, 283, 706, 776, 941, 1362
SchI GAGTC 5 cut(s) 307, 387, 641, 670, 913
ScrFI CCNGG 7 cut(s) 86, 91, 97, 202, 931, 1239, 1417
SduI GDGCHC 7 cut(s) 156, 329, 391, 651, 763, 802, 1392
SfaNI GCATC 6 cut(s) 282, 450, 512, 835, 1041, 1042
SfcI CTRYAG 1 cut(s) 521
SfuI TTCGAA 1 cut(s) 260
SinI GGWCC 4 cut(s) 93, 227, 706, 776
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 3 cut(s) 32, 896, 1276
SmoI CTYRAG 3 cut(s) 32, 896, 1276
Sse9I AATT 1 cut(s) 1078
SseBI AGGCCT 1 cut(s) 1102
SsiI CCGC 7 cut(s) 67, 73, 265, 310, 500, 689, 1030
SspMI CTAG 3 cut(s) 621, 812, 831
SstI GAGCTC 1 cut(s) 329
StuI AGGCCT 1 cut(s) 1102
StyD4I CCNGG 7 cut(s) 84, 89, 95, 200, 929, 1237, 1415
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 3 cut(s) 492, 750, 1046
TaiI ACGT 1 cut(s) 411
TaqI TCGA 5 cut(s) 260, 275, 459, 530, 1215
TasI AATT 1 cut(s) 1078
TatI WGTACW 2 cut(s) 477, 492
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 3 cut(s) 257, 1016, 1113
Tru1I TTAA 2 cut(s) 1230, 1277
Tru9I TTAA 2 cut(s) 1230, 1277
TscAI CASTG 3 cut(s) 293, 786, 1300
TseI GCWGC 6 cut(s) 81, 175, 301, 370, 520, 717
TspDTI ATGAA 6 cut(s) 617, 880, 995, 1071, 1249, 1335
TspGWI ACGGA 5 cut(s) 159, 255, 310, 319, 1129
TspRI CASTG 3 cut(s) 293, 786, 1300
Van91I CCANNNNNTGG 1 cut(s) 169
Vha464I CTTAAG 1 cut(s) 1276
VpaK11BI GGWCC 4 cut(s) 93, 227, 706, 776
XagI CCTNNNNNAGG 1 cut(s) 1107
XbaI TCTAGA 2 cut(s) 620, 811
XceI RCATGY 2 cut(s) 673, 965
XcmI CCANNNNNNNNNTGG 3 cut(s) 146, 1043, 1192
XspI CTAG 3 cut(s) 621, 812, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.