Rh6DG057800

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002346
Physical Location & Seq
Reverse (-)
5950012 .. 5953819
3808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG057800.1

Sequence Viewer

Length: 1428 bp
ATGGAGAAGAAGCAAGGGTTCTTCTCTGCTCTTAAAGACGAGGTGGTTCGCGGCCTCAGCCCCTCCCGCTCCCGCGCCTCCAGCCCCGCCCGCTCCGCCTCCCCCATGTCGGCTCTGCTCCGCCGCAAGAAGCACGGTCACAGCAACGGCCACGGCCAGCTGGTCGCGCAGCCGGAGCCCTTGATCGGGAGATCCGGCAGCCTCAGGCCGGTCATGGAGGGTCCGGATCCGGACGGCGGGGAAATCGGGGAGTCGAAGCGGGCCGGGTCGGGTCTGGGCCAGTGGATGCGCGGGCAGCTTTCCCGGACTCCTTCGGTGAGTTCAGTGGCGCAGAACAACCGGAGGTCTGATCTGAGACTCTTGCTCGGAGTCATGGGAGCTCCGCTCGCCCCCCTCCACGTCAGCACCTCCGACCCTCTCCCTCACCTCAGCATCAAGGACACTCCCATCGAAACTTCCTCTGCCCAGTACATATTGCAACAGTACACAGCGGCATCTGGTGGGCAGAAGCTGCAGAACTCGATTAAAAATGCGTATGCAATGGGAAAGGTTAGGATGGTAGCTTCTGAGTTTGAAACTGCCACCAAGGTGATGAAGACCAGAAACGCATCTAGATGTGCAGAGTCGGGTGGGTTTGTGCTCTGGCAGATGAATCCGGACATGTGGTATGTGGAGCTTGCGGTTGGGGGCAGCAAAGTGCATGCTGGCTGCAATGGGAAGCTCGTTTGGAGACACACGCCGTGGCAGGGTGCACATACTGCGAAAGGGCCTGTGAGACCCTTGCGTCGTGCTCTTCAGGGTCTTGATCCAAGATCTACAGCGAGTATGTTTGCTGACGCCAGATGTATTGGAGAAAGAAAGGTCAATGGGGAGGATTGCTTCATCCTCAAGCTCTGTGCTGATCCTCAAACTCTGAAGGCCAGGAGTGAAGGCCCTGCAGAGATAATTAGACATGTATTGTTTGGCTACTTCAGCCAGAAGACGGGGCTTCTTGTTCACATGGAGGACTCTCATTTGACCCGCATTCAATCCAATGGTGGTGATGCTGTTTACTGGGAAACCACAATCAATTCGTTCCTTGATGACTACAGACCTGTTGAGGGAATCATGATTGCGCACTCCGGGCGTTCTGTGGTTACCCTTTTCAGGTTCGGCGAGATGGCAATGAGCCATACCAAAACAAAGATGGAAGAAGCTTGGACAATTGAAGAGGTTGCATTTAATGTTCCAGGCTTGTCAATGGACTGCTTCATCCCCCCAGCTGACTTGAGATCTGAGACAATAATGAGTGAAGCATGCGAGCTTCCTCATGAGGAAAGGGGAAAGGGTGGGGGAATTGCAGCAGCAGTGCATCGGGCCAAAGTAGCTGCCTTGGAGAAAGAGCATAGTGTGAATGGTGATAGCATGAGCTGGAAGATGGAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

475

Amino Acids

51.8

Weight (kDa)

9.14

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 179 - 420 5.8e-122 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 783
Acc16I TGCGCA 1 cut(s) 1116
AccBSI CCGCTC 3 cut(s) 69, 93, 385
AccII CGCG 4 cut(s) 51, 75, 167, 291
AccIII TCCGGA 3 cut(s) 223, 229, 655
AclWI GGATC 5 cut(s) 186, 221, 234, 800, 896
AcoI YGGCCR 2 cut(s) 148, 154
AcuI CTGAAG 3 cut(s) 779, 935, 955
AcyI GRCGYC 1 cut(s) 837
AdeI CACNNNGTG 1 cut(s) 741
AfaI GTAC 2 cut(s) 470, 485
AfiI CCNNNNNNNGG 9 cut(s) 109, 185, 186, 208, 236, 746, 982, 1100, 1146
AflIII ACRYGT 2 cut(s) 660, 952
AgsI TTSAA 3 cut(s) 575, 1028, 1208
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 2 cut(s) 920, 1228
AleI CACNNNNGTG 1 cut(s) 587
Alw21I GWGCWC 4 cut(s) 382, 642, 754, 793
Alw26I GTCTC 4 cut(s) 349, 724, 769, 1271
Alw44I GTGCAC 1 cut(s) 750
AlwI GGATC 5 cut(s) 186, 221, 234, 800, 896
AlwNI CAGNNNCTG 1 cut(s) 511
Aor13HI TCCGGA 3 cut(s) 223, 229, 655
ApaLI GTGCAC 1 cut(s) 750
ApeKI GCWGC 9 cut(s) 169, 198, 295, 511, 690, 708, 1340, 1343, 1367
AspLEI GCGC 5 cut(s) 77, 169, 291, 331, 1117
AspS9I GGNCC 6 cut(s) 221, 261, 277, 767, 932, 1356
AsuC2I CCSGG 3 cut(s) 265, 304, 1123
AsuHPI GGTGA 5 cut(s) 328, 416, 601, 1052, 1409
AvaII GGWCC 1 cut(s) 221
AxyI CCTNAGG 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 754
BamHI GGATCC 1 cut(s) 226
BanII GRGCYC 2 cut(s) 180, 382
BbsI GAAGAC 2 cut(s) 602, 986
Bbv12I GWGCWC 4 cut(s) 382, 642, 754, 793
BbvCI CCTCAGC 2 cut(s) 56, 428
BbvI GCAGC 9 cut(s) 181, 210, 307, 498, 695, 702, 1352, 1354, 1355
BccI CCATC 5 cut(s) 455, 550, 1153, 1180, 1411
BceAI ACGGC 4 cut(s) 163, 169, 250, 724
BciT130I CCWGG 2 cut(s) 922, 1230
BcnI CCSGG 3 cut(s) 265, 304, 1123
BcoDI GTCTC 4 cut(s) 349, 724, 769, 1271
BfaI CTAG 1 cut(s) 612
BfmI CTRYAG 4 cut(s) 512, 816, 936, 1087
BglII AGATCT 2 cut(s) 812, 1271
Bme1390I CCNGG 5 cut(s) 265, 304, 922, 1123, 1230
Bme18I GGWCC 1 cut(s) 221
BmgBI CACGTC 1 cut(s) 400
BmgT120I GGNCC 6 cut(s) 221, 261, 277, 767, 932, 1356
BmiI GGNNCC 3 cut(s) 177, 222, 228
BmrFI CCNGG 5 cut(s) 265, 304, 922, 1123, 1230
BmrI ACTGGG 2 cut(s) 460, 1063
BmsI GCATC 6 cut(s) 276, 441, 503, 617, 1033, 1360
BmuI ACTGGG 2 cut(s) 460, 1063
BpiI GAAGAC 2 cut(s) 602, 986
BplI GAGNNNNNCTC 2 cut(s) 369, 401
BpmI CTGGAG 1 cut(s) 64
Bpu10I CCTNAGC 2 cut(s) 56, 428
BpuEI CTTGAG 2 cut(s) 872, 1288
BpuMI CCSGG 3 cut(s) 265, 304, 1123
BsaBI GATNNNNATC 1 cut(s) 1421
BsaHI GRCGYC 1 cut(s) 837
BsaI GGTCTC 1 cut(s) 769
BsaJI CCNNGG 4 cut(s) 151, 585, 740, 1371
BsaWI WCCGGW 4 cut(s) 223, 229, 339, 655
Bsc4I CCNNNNNNNGG 9 cut(s) 109, 185, 186, 208, 236, 746, 982, 1100, 1146
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 3 cut(s) 280, 466, 1058
Bse21I CCTNAGG 1 cut(s) 203
Bse3DI GCAATG 3 cut(s) 546, 718, 1170
Bse8I GATNNNNATC 1 cut(s) 1421
BseAI TCCGGA 3 cut(s) 223, 229, 655
BseBI CCWGG 2 cut(s) 922, 1230
BseDI CCNNGG 4 cut(s) 151, 585, 740, 1371
BseGI GGATG 4 cut(s) 291, 561, 882, 1251
BseJI GATNNNNATC 1 cut(s) 1421
BseLI CCNNNNNNNGG 9 cut(s) 109, 185, 186, 208, 236, 746, 982, 1100, 1146
BseMI GCAATG 3 cut(s) 546, 718, 1170
BseMII CTCAG 6 cut(s) 70, 217, 344, 442, 558, 1266
BseNI ACTGG 3 cut(s) 280, 466, 1058
BseSI GKGCMC 1 cut(s) 754
BseXI GCAGC 9 cut(s) 181, 210, 307, 498, 695, 702, 1352, 1354, 1355
BseYI CCCAGC 1 cut(s) 1258
BsgI GTGCAG 1 cut(s) 639
Bsh1236I CGCG 4 cut(s) 51, 75, 167, 291
BsiHKAI GWGCWC 4 cut(s) 382, 642, 754, 793
BslI CCNNNNNNNGG 9 cut(s) 109, 185, 186, 208, 236, 746, 982, 1100, 1146
BsmAI GTCTC 4 cut(s) 349, 724, 769, 1271
BsmI GAATGC 1 cut(s) 1023
Bso31I GGTCTC 1 cut(s) 769
Bsp1286I GDGCHC 5 cut(s) 180, 382, 642, 754, 793
Bsp13I TCCGGA 3 cut(s) 223, 229, 655
Bsp143I GATC 8 cut(s) 183, 191, 226, 349, 805, 812, 901, 1271
BspCNI CTCAG 6 cut(s) 69, 216, 345, 441, 559, 1267
BspEI TCCGGA 3 cut(s) 223, 229, 655
BspFNI CGCG 4 cut(s) 51, 75, 167, 291
BspHI TCATGA 2 cut(s) 1107, 1309
BspLI GGNNCC 3 cut(s) 177, 222, 228
BspMAI CTGCAG 2 cut(s) 516, 940
BspPI GGATC 5 cut(s) 186, 221, 234, 800, 896
BspQI GCTCTTC 1 cut(s) 798
BspTNI GGTCTC 1 cut(s) 769
BsrBI CCGCTC 3 cut(s) 69, 93, 385
BsrDI GCAATG 3 cut(s) 546, 718, 1170
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 3 cut(s) 280, 466, 1058
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 4 cut(s) 151, 585, 740, 1371
BssMI GATC 8 cut(s) 183, 191, 226, 349, 805, 812, 901, 1271
BssNI GRCGYC 1 cut(s) 837
BssT1I CCWWGG 2 cut(s) 585, 1371
Bst2UI CCWGG 2 cut(s) 922, 1230
Bst4CI ACNGT 2 cut(s) 137, 483
Bst6I CTCTTC 2 cut(s) 798, 1203
BstACI GRCGYC 1 cut(s) 837
BstAPI GCANNNNNTGC 2 cut(s) 511, 758
BstDEI CTNAG 6 cut(s) 56, 203, 353, 428, 567, 1275
BstDSI CCRYGG 2 cut(s) 151, 740
BstEII GGTNACC 1 cut(s) 1135
BstF5I GGATG 4 cut(s) 291, 561, 882, 1251
BstFNI CGCG 4 cut(s) 51, 75, 167, 291
BstHHI GCGC 5 cut(s) 77, 169, 291, 331, 1117
BstKTI GATC 8 cut(s) 186, 194, 229, 352, 808, 815, 904, 1274
BstMAI GTCTC 4 cut(s) 349, 724, 769, 1271
BstMBI GATC 8 cut(s) 183, 191, 226, 349, 805, 812, 901, 1271
BstNI CCWGG 2 cut(s) 922, 1230
BstNSI RCATGY 4 cut(s) 664, 704, 956, 1299
BstPI GGTNACC 1 cut(s) 1135
BstSCI CCNGG 5 cut(s) 263, 302, 920, 1121, 1228
BstSFI CTRYAG 4 cut(s) 512, 816, 936, 1087
BstSLI GKGCMC 1 cut(s) 754
BstUI CGCG 4 cut(s) 51, 75, 167, 291
BstV1I GCAGC 9 cut(s) 181, 210, 307, 498, 695, 702, 1352, 1354, 1355
BstV2I GAAGAC 2 cut(s) 602, 986
BstX2I RGATCY 4 cut(s) 191, 226, 812, 1271
BstYI RGATCY 4 cut(s) 191, 226, 812, 1271
Bsu36I CCTNAGG 1 cut(s) 203
BtgI CCRYGG 2 cut(s) 151, 740
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 4 cut(s) 291, 561, 882, 1251
BtsI GCAGTG 1 cut(s) 1353
BtsIMutI CAGTG 3 cut(s) 287, 330, 1353
CaiI CAGNNNCTG 1 cut(s) 511
CciI TCATGA 2 cut(s) 1107, 1309
CfoI GCGC 5 cut(s) 77, 169, 291, 331, 1117
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 6 cut(s) 221, 261, 277, 767, 932, 1356
CseI GACGC 2 cut(s) 773, 845
Csp6I GTAC 2 cut(s) 469, 484
CviQI GTAC 2 cut(s) 469, 484
DdeI CTNAG 6 cut(s) 56, 203, 353, 428, 567, 1275
DpnI GATC 8 cut(s) 185, 193, 228, 351, 807, 814, 903, 1273
DpnII GATC 8 cut(s) 183, 191, 226, 349, 805, 812, 901, 1271
DraIII CACNNNGTG 1 cut(s) 741
DrdI GACNNNNNNGTC 1 cut(s) 783
DseDI GACNNNNNNGTC 1 cut(s) 783
EaeI YGGCCR 2 cut(s) 148, 154
Eam1104I CTCTTC 2 cut(s) 798, 1203
EarI CTCTTC 2 cut(s) 798, 1203
EciI GGCGGA 2 cut(s) 85, 110
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 2 cut(s) 585, 1371
Eco24I GRGCYC 2 cut(s) 180, 382
Eco31I GGTCTC 1 cut(s) 769
Eco47I GGWCC 1 cut(s) 221
Eco53kI GAGCTC 1 cut(s) 380
Eco57I CTGAAG 3 cut(s) 779, 935, 955
Eco81I CCTNAGG 1 cut(s) 203
Eco91I GGTNACC 1 cut(s) 1135
EcoICRI GAGCTC 1 cut(s) 380
EcoO109I RGGNCCY 2 cut(s) 767, 932
EcoO65I GGTNACC 1 cut(s) 1135
EcoRII CCWGG 2 cut(s) 920, 1228
EcoT14I CCWWGG 2 cut(s) 585, 1371
EcoT38I GRGCYC 2 cut(s) 180, 382
ErhI CCWWGG 2 cut(s) 585, 1371
FauI CCCGC 8 cut(s) 74, 80, 94, 98, 230, 252, 284, 1028
FokI GGATG 4 cut(s) 298, 568, 869, 1238
FriOI GRGCYC 2 cut(s) 180, 382
FspBI CTAG 1 cut(s) 612
FspI TGCGCA 1 cut(s) 1116
GlaI GCGC 5 cut(s) 76, 168, 290, 330, 1116
GsaI CCCAGC 1 cut(s) 1262
GsuI CTGGAG 1 cut(s) 64
HgaI GACGC 2 cut(s) 773, 845
HhaI GCGC 5 cut(s) 77, 169, 291, 331, 1117
Hin1I GRCGYC 1 cut(s) 837
Hin6I GCGC 5 cut(s) 75, 167, 289, 329, 1115
HinP1I GCGC 5 cut(s) 75, 167, 289, 329, 1115
HindIII AAGCTT 1 cut(s) 1194
HinfI GANTC 8 cut(s) 251, 307, 357, 369, 623, 652, 1007, 1104
HphI GGTGA 5 cut(s) 328, 416, 601, 1052, 1409
Hpy166II GTNNAC 4 cut(s) 486, 752, 997, 1051
Hpy188I TCNGA 7 cut(s) 349, 354, 368, 412, 568, 915, 1276
Hpy188III TCNNGA 8 cut(s) 187, 224, 230, 612, 656, 803, 1108, 1310
Hpy8I GTNNAC 4 cut(s) 486, 752, 997, 1051
Hpy99I CGWCG 1 cut(s) 789
HpyAV CCTTC 3 cut(s) 321, 910, 923
HpyCH4III ACNGT 2 cut(s) 137, 483
HpyCH4IV ACGT 1 cut(s) 399
HpyF3I CTNAG 6 cut(s) 56, 203, 353, 428, 567, 1275
HpySE526I ACGT 1 cut(s) 399
Hsp92I GRCGYC 1 cut(s) 837
HspAI GCGC 5 cut(s) 75, 167, 289, 329, 1115
Kpn2I TCCGGA 3 cut(s) 223, 229, 655
Kzo9I GATC 8 cut(s) 183, 191, 226, 349, 805, 812, 901, 1271
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 7 cut(s) 74, 98, 123, 175, 377, 385, 673
Lsp1109I GCAGC 9 cut(s) 181, 210, 307, 498, 695, 702, 1352, 1354, 1355
LweI GCATC 6 cut(s) 276, 441, 503, 617, 1033, 1360
MaeI CTAG 1 cut(s) 612
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 2 cut(s) 137, 1135
MalI GATC 8 cut(s) 185, 193, 228, 351, 807, 814, 903, 1273
MbiI CCGCTC 3 cut(s) 69, 93, 385
MboI GATC 8 cut(s) 183, 191, 226, 349, 805, 812, 901, 1271
MboII GAAGA 8 cut(s) 13, 19, 607, 785, 991, 1202, 1220, 1426
MfeI CAATTG 1 cut(s) 1203
MflI RGATCY 4 cut(s) 191, 226, 812, 1271
MhlI GDGCHC 5 cut(s) 180, 382, 642, 754, 793
MluCI AATT 4 cut(s) 945, 1069, 1203, 1335
MlyI GAGTC 6 cut(s) 260, 301, 351, 378, 632, 1001
MmeI TCCRAC 1 cut(s) 435
MroI TCCGGA 3 cut(s) 223, 229, 655
MseI TTAA 3 cut(s) 33, 525, 1221
MslI CAYNNNNRTG 2 cut(s) 587, 613
MspA1I CMGCKG 3 cut(s) 160, 491, 1262
MspR9I CCNGG 5 cut(s) 265, 304, 922, 1123, 1230
MunI CAATTG 1 cut(s) 1203
Mva1269I GAATGC 1 cut(s) 1023
MvaI CCWGG 2 cut(s) 922, 1230
MvnI CGCG 4 cut(s) 51, 75, 167, 291
NciI CCSGG 3 cut(s) 265, 304, 1123
NdeII GATC 8 cut(s) 183, 191, 226, 349, 805, 812, 901, 1271
NlaIV GGNNCC 3 cut(s) 177, 222, 228
NmuCI GTSAC 1 cut(s) 137
NsbI TGCGCA 1 cut(s) 1116
NspI RCATGY 4 cut(s) 664, 704, 956, 1299
OliI CACNNNNGTG 1 cut(s) 587
PaeI GCATGC 2 cut(s) 704, 1299
PagI TCATGA 2 cut(s) 1107, 1309
PciI ACATGT 2 cut(s) 660, 952
PciSI GCTCTTC 1 cut(s) 798
PctI GAATGC 1 cut(s) 1023
PfeI GAWTC 2 cut(s) 652, 1104
PfoI TCCNGGA 1 cut(s) 302
PleI GAGTC 6 cut(s) 259, 301, 351, 377, 631, 1001
PpsI GAGTC 6 cut(s) 259, 301, 351, 377, 631, 1001
PscI ACATGT 2 cut(s) 660, 952
Psp124BI GAGCTC 1 cut(s) 382
Psp6I CCWGG 2 cut(s) 920, 1228
PspEI GGTNACC 1 cut(s) 1135
PspFI CCCAGC 1 cut(s) 1258
PspGI CCWGG 2 cut(s) 920, 1228
PspN4I GGNNCC 3 cut(s) 177, 222, 228
PspPI GGNCC 6 cut(s) 221, 261, 277, 767, 932, 1356
PstI CTGCAG 2 cut(s) 516, 940
PstNI CAGNNNCTG 1 cut(s) 511
PsuI RGATCY 4 cut(s) 191, 226, 812, 1271
PvuII CAGCTG 2 cut(s) 160, 1262
RsaI GTAC 2 cut(s) 470, 485
RsaNI GTAC 2 cut(s) 469, 484
RseI CAYNNNNRTG 2 cut(s) 587, 613
SacI GAGCTC 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 3 cut(s) 33, 525, 1221
Sau3AI GATC 8 cut(s) 183, 191, 226, 349, 805, 812, 901, 1271
Sau96I GGNCC 6 cut(s) 221, 261, 277, 767, 932, 1356
SchI GAGTC 6 cut(s) 260, 301, 351, 378, 632, 1001
ScrFI CCNGG 5 cut(s) 265, 304, 922, 1123, 1230
SduI GDGCHC 5 cut(s) 180, 382, 642, 754, 793
SfaNI GCATC 6 cut(s) 276, 441, 503, 617, 1033, 1360
SfcI CTRYAG 4 cut(s) 512, 816, 936, 1087
SinI GGWCC 1 cut(s) 221
SmiMI CAYNNNNRTG 2 cut(s) 587, 613
SmlI CTYRAG 2 cut(s) 887, 1267
SmoI CTYRAG 2 cut(s) 887, 1267
SphI GCATGC 2 cut(s) 704, 1299
Sse9I AATT 4 cut(s) 945, 1069, 1203, 1335
SspMI CTAG 1 cut(s) 612
SstI GAGCTC 1 cut(s) 382
StyD4I CCNGG 5 cut(s) 263, 302, 920, 1121, 1228
StyI CCWWGG 2 cut(s) 585, 1371
TaaI ACNGT 2 cut(s) 137, 483
TaiI ACGT 1 cut(s) 402
TaqI TCGA 3 cut(s) 254, 450, 521
TasI AATT 4 cut(s) 945, 1069, 1203, 1335
TatI WGTACW 2 cut(s) 468, 483
TauI GCSGC 3 cut(s) 54, 126, 494
TfiI GAWTC 2 cut(s) 652, 1104
Tru1I TTAA 3 cut(s) 33, 525, 1221
Tru9I TTAA 3 cut(s) 33, 525, 1221
TscAI CASTG 3 cut(s) 287, 330, 1353
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 9 cut(s) 169, 198, 295, 511, 690, 708, 1340, 1343, 1367
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 4 cut(s) 608, 665, 871, 1240
TspRI CASTG 3 cut(s) 287, 330, 1353
VneI GTGCAC 1 cut(s) 750
VpaK11BI GGWCC 1 cut(s) 221
XbaI TCTAGA 1 cut(s) 611
XceI RCATGY 4 cut(s) 664, 704, 956, 1299
XcmI CCANNNNNNNNNTGG 1 cut(s) 1183
XspI CTAG 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.