AT3G22530

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007781
Physical Location & Seq
Reverse (-)
7977604 .. 7978635
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22530.1

Sequence Viewer

Length: 597 bp
ATGATTTTGATTCCCGTAAAGATTCAATCACTTTTCTCATATCTCTTGTTACTGAATTTGATCCAATCGATCGATAAACCAAACCCAGAAGCTATGAGAGTGCACCCAATTCCCAGAAACATCAACAACACATTGATCCATCATCATCATCATAACCCGACCCGAGAACCCGGAAAGAATCTCCGACGATTACCACATATCTTCAACCGTGTTCTGGAGCTACCATTGAGGTCAGAAGCAGATGTAGCAGTGGAAGAGAGACACGACTGTTTTCGATTTGTGGCGGAGACGGTAGGTCTCTGTAACGGCGATGGAGAAATGAGGGCTTATATGGTTGAGATTCATCCTGGTATAACCAAGATCGTTGTGAGGACAAATGGGTCTTCTTCTCTTGGTTTGTCTTTAGACGAATTAGAGCTTGACGTGTGGCGTTTTAGGTTACCGGAATCAACTAGGCCTGAGCTTGTTACTGTTGCTTGTGTTGATGGTGATTTGATTGTTACTGTGCCTAAGAATGCTGAAGAAGAAGATGATGATGGTGGTGGTGGTGATTTTGGACAAGGTATTGGTAGTGGAAGGCTTGTGCTTGTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.12

Weight (kDa)

5.56

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 379
AciI CCGC 1 cut(s) 284
AclWI GGATC 2 cut(s) 55, 130
AcsI RAATTY 1 cut(s) 55
AcuI CTGAAG 1 cut(s) 540
AfiI CCNNNNNNNGG 1 cut(s) 214
AflIII ACRYGT 1 cut(s) 423
AgsI TTSAA 2 cut(s) 26, 205
AhdI GACNNNNNGTC 1 cut(s) 294
AjiI CACGTC 1 cut(s) 424
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 4 cut(s) 92, 220, 418, 463
AluI AGCT 4 cut(s) 92, 220, 418, 463
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 3 cut(s) 253, 281, 302
Alw44I GTGCAC 1 cut(s) 101
AlwI GGATC 2 cut(s) 55, 130
Ama87I CYCGRG 1 cut(s) 162
AoxI GGCC 1 cut(s) 455
ApaLI GTGCAC 1 cut(s) 101
ApoI RAATTY 1 cut(s) 55
AsuC2I CCSGG 1 cut(s) 171
AsuHPI GGTGA 2 cut(s) 500, 560
AvaI CYCGRG 1 cut(s) 162
BaeGI GKGCMC 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 105
BccI CCATC 4 cut(s) 147, 305, 479, 530
BceAI ACGGC 1 cut(s) 322
BciT130I CCWGG 1 cut(s) 348
BcnI CCSGG 1 cut(s) 171
BcoDI GTCTC 3 cut(s) 253, 281, 302
BfaI CTAG 1 cut(s) 453
Bme1390I CCNGG 2 cut(s) 171, 348
BmeRI GACNNNNNGTC 1 cut(s) 294
BmeT110I CYCGRG 1 cut(s) 162
BmgBI CACGTC 1 cut(s) 424
BmrFI CCNGG 2 cut(s) 171, 348
BpiI GAAGAC 1 cut(s) 375
BpmI CTGGAG 1 cut(s) 236
Bpu10I CCTNAGC 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 171
Bsa29I ATCGAT 2 cut(s) 68, 72
BsaI GGTCTC 1 cut(s) 302
BsaWI WCCGGW 1 cut(s) 442
Bsc4I CCNNNNNNNGG 1 cut(s) 214
BseBI CCWGG 1 cut(s) 348
BseCI ATCGAT 2 cut(s) 68, 72
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMII CTCAG 1 cut(s) 450
BseSI GKGCMC 1 cut(s) 105
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 1 cut(s) 457
BshVI ATCGAT 2 cut(s) 68, 72
BsiEI CGRYCG 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 162
BsiSI CCGG 2 cut(s) 171, 443
BslI CCNNNNNNNGG 1 cut(s) 214
BsmAI GTCTC 3 cut(s) 253, 281, 302
BsmBI CGTCTC 1 cut(s) 281
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 1 cut(s) 457
Bso31I GGTCTC 1 cut(s) 302
BsoBI CYCGRG 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 4 cut(s) 60, 69, 135, 360
BspACI CCGC 1 cut(s) 284
BspANI GGCC 1 cut(s) 457
BspCNI CTCAG 1 cut(s) 451
BspDI ATCGAT 2 cut(s) 68, 72
BspPI GGATC 2 cut(s) 55, 130
BspTNI GGTCTC 1 cut(s) 302
BssMI GATC 4 cut(s) 60, 69, 135, 360
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 5 cut(s) 209, 269, 292, 472, 505
Bst6I CTCTTC 1 cut(s) 249
BstDEI CTNAG 2 cut(s) 459, 510
BstEII GGTNACC 1 cut(s) 438
BstF5I GGATG 1 cut(s) 343
BstKTI GATC 4 cut(s) 63, 72, 138, 363
BstMAI GTCTC 3 cut(s) 253, 281, 302
BstMBI GATC 4 cut(s) 60, 69, 135, 360
BstMCI CGRYCG 1 cut(s) 72
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNI CCWGG 1 cut(s) 348
BstPI GGTNACC 1 cut(s) 438
BstSCI CCNGG 2 cut(s) 169, 346
BstSLI GKGCMC 1 cut(s) 105
BstV2I GAAGAC 1 cut(s) 375
Bsu15I ATCGAT 2 cut(s) 68, 72
BsuRI GGCC 1 cut(s) 457
BsuTUI ATCGAT 2 cut(s) 68, 72
BtgZI GCGATG 1 cut(s) 324
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 1 cut(s) 343
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 255
ClaI ATCGAT 2 cut(s) 68, 72
CviJI RGCY 7 cut(s) 92, 220, 326, 418, 457, 463, 580
CviKI_1 RGCY 7 cut(s) 92, 220, 326, 418, 457, 463, 580
DdeI CTNAG 2 cut(s) 459, 510
DpnI GATC 4 cut(s) 62, 71, 137, 362
DpnII GATC 4 cut(s) 60, 69, 135, 360
DrdI GACNNNNNNGTC 1 cut(s) 379
DriI GACNNNNNGTC 1 cut(s) 294
DseDI GACNNNNNNGTC 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 249
Eam1105I GACNNNNNGTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 249
EciI GGCGGA 1 cut(s) 299
Eco147I AGGCCT 1 cut(s) 457
Eco31I GGTCTC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 540
Eco88I CYCGRG 1 cut(s) 162
Eco91I GGTNACC 1 cut(s) 438
EcoO65I GGTNACC 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 346
Esp3I CGTCTC 1 cut(s) 281
FaiI YATR 7 cut(s) 40, 95, 153, 198, 330, 332, 353
FokI GGATG 1 cut(s) 330
FspBI CTAG 1 cut(s) 453
GsuI CTGGAG 1 cut(s) 236
HaeIII GGCC 1 cut(s) 457
HapII CCGG 2 cut(s) 171, 443
HinfI GANTC 5 cut(s) 10, 22, 178, 340, 446
HpaII CCGG 2 cut(s) 171, 443
HphI GGTGA 2 cut(s) 500, 560
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 2 cut(s) 185, 235
Hpy188III TCNNGA 1 cut(s) 215
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 1 cut(s) 189
HpyAV CCTTC 1 cut(s) 570
HpyCH4III ACNGT 5 cut(s) 209, 269, 292, 472, 505
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 1 cut(s) 103
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 2 cut(s) 459, 510
HpySE526I ACGT 1 cut(s) 423
Kzo9I GATC 4 cut(s) 60, 69, 135, 360
LmnI GCTCC 1 cut(s) 217
LpnPI CCDG 8 cut(s) 99, 127, 184, 200, 333, 360, 456, 471
MaeI CTAG 1 cut(s) 453
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 5 cut(s) 48, 302, 438, 466, 499
MalI GATC 4 cut(s) 62, 71, 137, 362
MboI GATC 4 cut(s) 60, 69, 135, 360
MboII GAAGA 7 cut(s) 193, 266, 375, 378, 533, 536, 539
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 3 cut(s) 55, 108, 410
MmeI TCCRAC 1 cut(s) 208
MnlI CCTC 3 cut(s) 222, 315, 363
MspI CCGG 2 cut(s) 171, 443
MspR9I CCNGG 2 cut(s) 171, 348
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 1 cut(s) 348
MwoI GCNNNNNNNGC 1 cut(s) 245
NciI CCSGG 1 cut(s) 171
NdeII GATC 4 cut(s) 60, 69, 135, 360
PceI AGGCCT 1 cut(s) 457
PctI GAATGC 1 cut(s) 520
PfeI GAWTC 5 cut(s) 10, 22, 178, 340, 446
Ple19I CGATCG 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 346
PspEI GGTNACC 1 cut(s) 438
PspGI CCWGG 1 cut(s) 346
PvuI CGATCG 1 cut(s) 72
Sau3AI GATC 4 cut(s) 60, 69, 135, 360
ScrFI CCNGG 2 cut(s) 171, 348
SduI GDGCHC 1 cut(s) 105
SetI ASST 9 cut(s) 94, 222, 233, 298, 420, 426, 440, 465, 565
Sse9I AATT 3 cut(s) 55, 108, 410
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 1 cut(s) 284
SspMI CTAG 1 cut(s) 453
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 2 cut(s) 169, 346
TaaI ACNGT 5 cut(s) 209, 269, 292, 472, 505
TaiI ACGT 1 cut(s) 426
TaqI TCGA 3 cut(s) 68, 72, 274
TasI AATT 3 cut(s) 55, 108, 410
TfiI GAWTC 5 cut(s) 10, 22, 178, 340, 446
TscAI CASTG 1 cut(s) 255
TspDTI ATGAA 1 cut(s) 332
TspRI CASTG 1 cut(s) 255
VneI GTGCAC 1 cut(s) 101
XapI RAATTY 1 cut(s) 55
XspI CTAG 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.