pycom16g16460

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007781
Physical Location & Seq
Reverse (-)
12365382 .. 12366211
830 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16460.2

Sequence Viewer

Length: 750 bp
ATGATTCCTCCGTTTGGTAAAAACAACCACCCAATCAGGATTCTGATTCCCACCCAAGGTTGGACTGCCTTTTCCAGAAGAAGGGCATCTTTCATCTTTCTATTAATCCTGTCCCAATCCCTCTATTTCTTCTGCACAACCACCGCCACTGCCGCCGCTGCCGCCGTAGCTGCCACTGAAACCGCCCAAACCATGAAGGTCCACCCCATGCCCAGGAAGCGCAACATCACCGTCCAATACAGCTCAAGAAACTCACTTTCTCAAGCGCAGGCACTTTTGGGTCCTAACCACAAGAAGCTCCGCCGTCTCCCCCATGTCTTCAGCCGGGTCCTGGAGCTCCCCTTTCGATCCGACGCCGACGTTCTGGTCCAGGAGAACCCCGATTGCTTCCGATTTGTCGCCGAAGCCGATAATATCGGCGACGACGTCAGGACCCATACCGTCGAAATCCATCCCGGGGTGACCAAAATCGTCGTTCGGGAAAGTGGGTCGGTGGAATTAACGTTGGACGAGCTCGAACTCGATATGTGGAGGTTTAGGCTGCCGGAATCGACTCGGCCCGAGCTGGCCAGCGCGGTTTTTGTTGACGGGGAGCTTATCGTGACGGTGCCGAAGGCCGAGGGGGTGGGGAATTCGGACGGTGGTGATGGTGGCGGAGAGGTTTGGGGCGGTGGCGACGGGAATGGGGGCGGGGGTTTCAGAGGAGGTATGGGTAACCGCCTTGTGCTTGTACACTTGCAACCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

27.19

Weight (kDa)

7.07

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 365
AatII GACGTC 1 cut(s) 429
AccB1I GGYRCC 1 cut(s) 607
AccII CGCG 1 cut(s) 575
AclI AACGTT 1 cut(s) 503
AclWI GGATC 1 cut(s) 342
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 1 cut(s) 631
AcuI CTGAAG 1 cut(s) 304
AcyI GRCGYC 2 cut(s) 354, 426
AfaI GTAC 1 cut(s) 732
AfiI CCNNNNNNNGG 7 cut(s) 14, 56, 60, 81, 213, 331, 457
AjnI CCWGG 3 cut(s) 212, 330, 369
AluBI AGCT 7 cut(s) 170, 243, 298, 337, 514, 565, 595
AluI AGCT 7 cut(s) 170, 243, 298, 337, 514, 565, 595
Alw21I GWGCWC 2 cut(s) 339, 516
Alw26I GTCTC 1 cut(s) 311
AlwI GGATC 1 cut(s) 342
Ama87I CYCGRG 2 cut(s) 455, 560
AoxI GGCC 3 cut(s) 557, 567, 615
ApeKI GCWGC 3 cut(s) 158, 170, 541
ApoI RAATTY 1 cut(s) 631
AseI ATTAAT 1 cut(s) 104
AspLEI GCGC 3 cut(s) 222, 268, 575
AspS9I GGNCC 6 cut(s) 199, 281, 328, 367, 432, 558
AsuC2I CCSGG 3 cut(s) 326, 456, 457
AsuHPI GGTGA 3 cut(s) 220, 472, 656
AvaI CYCGRG 2 cut(s) 455, 560
AvaII GGWCC 5 cut(s) 199, 281, 328, 367, 432
BalI TGGCCA 1 cut(s) 569
BanI GGYRCC 1 cut(s) 607
BanII GRGCYC 2 cut(s) 339, 516
BbsI GAAGAC 1 cut(s) 310
Bbv12I GWGCWC 2 cut(s) 339, 516
BbvI GCAGC 3 cut(s) 145, 157, 528
BccI CCATC 2 cut(s) 459, 641
BceAI ACGGC 2 cut(s) 149, 288
BciT130I CCWGG 3 cut(s) 214, 332, 371
BcnI CCSGG 3 cut(s) 326, 456, 457
BcoDI GTCTC 1 cut(s) 311
BisI GCNGC 6 cut(s) 153, 156, 159, 162, 171, 542
BlsI GCNGC 6 cut(s) 154, 157, 160, 163, 172, 543
Bme1390I CCNGG 6 cut(s) 214, 326, 332, 371, 456, 457
Bme18I GGWCC 5 cut(s) 199, 281, 328, 367, 432
BmeT110I CYCGRG 2 cut(s) 455, 560
BmgT120I GGNCC 6 cut(s) 199, 281, 328, 367, 432, 558
BmiI GGNNCC 4 cut(s) 282, 329, 434, 609
BmrFI CCNGG 6 cut(s) 214, 326, 332, 371, 456, 457
BmsI GCATC 1 cut(s) 95
BpiI GAAGAC 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 353
BpuEI CTTGAG 2 cut(s) 229, 246
BpuMI CCSGG 3 cut(s) 326, 456, 457
BsaHI GRCGYC 2 cut(s) 354, 426
BsaJI CCNNGG 5 cut(s) 55, 212, 455, 456, 618
Bsc4I CCNNNNNNNGG 7 cut(s) 14, 56, 60, 81, 213, 331, 457
BseBI CCWGG 3 cut(s) 214, 332, 371
BseDI CCNNGG 5 cut(s) 55, 212, 455, 456, 618
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 7 cut(s) 14, 56, 60, 81, 213, 331, 457
BseRI GAGGAG 1 cut(s) 717
BseXI GCAGC 3 cut(s) 145, 157, 528
BsgI GTGCAG 1 cut(s) 118
Bsh1236I CGCG 1 cut(s) 575
BshFI GGCC 3 cut(s) 559, 569, 617
BshNI GGYRCC 1 cut(s) 607
BsiHKAI GWGCWC 2 cut(s) 339, 516
BsiHKCI CYCGRG 2 cut(s) 455, 560
BsiSI CCGG 3 cut(s) 325, 456, 545
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 7 cut(s) 14, 56, 60, 81, 213, 331, 457
BsmAI GTCTC 1 cut(s) 311
BsmBI CGTCTC 1 cut(s) 311
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 3 cut(s) 559, 569, 617
BsoBI CYCGRG 2 cut(s) 455, 560
Bsp1286I GDGCHC 2 cut(s) 339, 516
Bsp1407I TGTACA 1 cut(s) 730
Bsp143I GATC 1 cut(s) 347
BspANI GGCC 3 cut(s) 559, 569, 617
BspFNI CGCG 1 cut(s) 575
BspLI GGNNCC 4 cut(s) 282, 329, 434, 609
BspPI GGATC 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 607
BsrGI TGTACA 1 cut(s) 730
BssECI CCNNGG 5 cut(s) 55, 212, 455, 456, 618
BssMI GATC 1 cut(s) 347
BssNI GRCGYC 2 cut(s) 354, 426
BssT1I CCWWGG 1 cut(s) 55
Bst2UI CCWGG 3 cut(s) 214, 332, 371
Bst4CI ACNGT 4 cut(s) 232, 442, 607, 641
BstACI GRCGYC 2 cut(s) 354, 426
BstAUI TGTACA 1 cut(s) 730
BstC8I GCNNGC 3 cut(s) 270, 567, 571
BstEII GGTNACC 2 cut(s) 460, 713
BstF5I GGATG 1 cut(s) 451
BstFNI CGCG 1 cut(s) 575
BstHHI GCGC 3 cut(s) 222, 268, 575
BstKTI GATC 1 cut(s) 350
BstMAI GTCTC 1 cut(s) 311
BstMBI GATC 1 cut(s) 347
BstMWI GCNNNNNNNGC 6 cut(s) 152, 158, 161, 167, 170, 217
BstNI CCWGG 3 cut(s) 214, 332, 371
BstPI GGTNACC 2 cut(s) 460, 713
BstSCI CCNGG 6 cut(s) 212, 324, 330, 369, 454, 455
BstUI CGCG 1 cut(s) 575
BstV1I GCAGC 3 cut(s) 145, 157, 528
BstV2I GAAGAC 1 cut(s) 310
BsuRI GGCC 3 cut(s) 559, 569, 617
BtsCI GGATG 1 cut(s) 451
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 174
Cac8I GCNNGC 3 cut(s) 270, 567, 571
CfoI GCGC 3 cut(s) 222, 268, 575
Cfr13I GGNCC 6 cut(s) 199, 281, 328, 367, 432, 558
Cfr9I CCCGGG 1 cut(s) 455
CseI GACGC 1 cut(s) 362
Csp6I GTAC 1 cut(s) 731
CviAII CATG 3 cut(s) 193, 208, 314
CviQI GTAC 1 cut(s) 731
DpnI GATC 1 cut(s) 349
DpnII GATC 1 cut(s) 347
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
EaeI YGGCCR 1 cut(s) 567
EciI GGCGGA 2 cut(s) 290, 669
Ecl136II GAGCTC 2 cut(s) 337, 514
Eco130I CCWWGG 1 cut(s) 55
Eco24I GRGCYC 2 cut(s) 339, 516
Eco47I GGWCC 5 cut(s) 199, 281, 328, 367, 432
Eco53kI GAGCTC 2 cut(s) 337, 514
Eco57I CTGAAG 1 cut(s) 304
Eco88I CYCGRG 2 cut(s) 455, 560
Eco91I GGTNACC 2 cut(s) 460, 713
EcoICRI GAGCTC 2 cut(s) 337, 514
EcoO109I RGGNCCY 3 cut(s) 281, 328, 432
EcoO65I GGTNACC 2 cut(s) 460, 713
EcoRI GAATTC 1 cut(s) 631
EcoRII CCWGG 3 cut(s) 212, 330, 369
EcoT14I CCWWGG 1 cut(s) 55
EcoT38I GRGCYC 2 cut(s) 339, 516
ErhI CCWWGG 1 cut(s) 55
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 3 cut(s) 196, 211, 317
FaiI YATR 6 cut(s) 194, 209, 315, 438, 527, 710
FalI AAGNNNNNCTT 2 cut(s) 73, 105
FaqI GGGAC 1 cut(s) 97
FatI CATG 3 cut(s) 192, 207, 313
FauI CCCGC 1 cut(s) 683
Fnu4HI GCNGC 6 cut(s) 153, 156, 159, 162, 171, 542
FokI GGATG 1 cut(s) 438
FriOI GRGCYC 2 cut(s) 339, 516
Fsp4HI GCNGC 6 cut(s) 153, 156, 159, 162, 171, 542
GlaI GCGC 3 cut(s) 221, 267, 574
GluI GCNGC 6 cut(s) 153, 156, 159, 162, 171, 542
GsuI CTGGAG 1 cut(s) 353
HaeIII GGCC 3 cut(s) 559, 569, 617
HapII CCGG 3 cut(s) 325, 456, 545
HgaI GACGC 1 cut(s) 362
HhaI GCGC 3 cut(s) 222, 268, 575
Hin1I GRCGYC 2 cut(s) 354, 426
Hin1II CATG 3 cut(s) 196, 211, 317
Hin6I GCGC 3 cut(s) 220, 266, 573
HinP1I GCGC 3 cut(s) 220, 266, 573
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HinfI GANTC 5 cut(s) 4, 40, 46, 548, 553
HpaII CCGG 3 cut(s) 325, 456, 545
HphI GGTGA 3 cut(s) 220, 472, 656
Hpy166II GTNNAC 3 cut(s) 202, 586, 733
Hpy188I TCNGA 5 cut(s) 45, 352, 392, 637, 701
Hpy188III TCNNGA 6 cut(s) 37, 75, 246, 430, 479, 601
Hpy8I GTNNAC 3 cut(s) 202, 586, 733
Hpy99I CGWCG 7 cut(s) 356, 362, 425, 428, 446, 476, 680
HpyAV CCTTC 3 cut(s) 75, 190, 607
HpyCH4III ACNGT 4 cut(s) 232, 442, 607, 641
HpyCH4IV ACGT 3 cut(s) 360, 426, 503
HpyCH4V TGCA 2 cut(s) 135, 739
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 158, 161, 167, 170, 217
HpySE526I ACGT 3 cut(s) 360, 426, 503
Hsp92I GRCGYC 2 cut(s) 354, 426
Hsp92II CATG 3 cut(s) 196, 211, 317
HspAI GCGC 3 cut(s) 220, 266, 573
Kzo9I GATC 1 cut(s) 347
LmnI GCTCC 4 cut(s) 303, 334, 342, 592
Lsp1109I GCAGC 3 cut(s) 145, 157, 528
LweI GCATC 1 cut(s) 95
MaeII ACGT 3 cut(s) 360, 426, 503
MaeIII GTNAC 3 cut(s) 460, 601, 713
MalI GATC 1 cut(s) 349
MboI GATC 1 cut(s) 347
MboII GAAGA 3 cut(s) 90, 121, 310
MhlI GDGCHC 2 cut(s) 339, 516
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 2 cut(s) 497, 631
MluNI TGGCCA 1 cut(s) 569
MlyI GAGTC 1 cut(s) 547
MmeI TCCRAC 3 cut(s) 41, 375, 486
MnlI CCTC 7 cut(s) 18, 131, 525, 613, 652, 695, 698
Mox20I TGGCCA 1 cut(s) 569
MscI TGGCCA 1 cut(s) 569
MseI TTAA 2 cut(s) 104, 500
Msp20I TGGCCA 1 cut(s) 569
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 3 cut(s) 325, 456, 545
MspR9I CCNGG 6 cut(s) 214, 326, 332, 371, 456, 457
MvaI CCWGG 3 cut(s) 214, 332, 371
MvnI CGCG 1 cut(s) 575
MwoI GCNNNNNNNGC 6 cut(s) 152, 158, 161, 167, 170, 217
NciI CCSGG 3 cut(s) 326, 456, 457
NdeII GATC 1 cut(s) 347
NlaIII CATG 3 cut(s) 196, 211, 317
NlaIV GGNNCC 4 cut(s) 282, 329, 434, 609
NmeAIII GCCGAG 2 cut(s) 535, 643
NmuCI GTSAC 2 cut(s) 460, 601
PcsI WCGNNNNNNNCGW 2 cut(s) 405, 423
PfeI GAWTC 4 cut(s) 4, 40, 46, 548
PflFI GACNNNGTC 1 cut(s) 425
PfoI TCCNGGA 2 cut(s) 330, 369
PkrI GCNGC 6 cut(s) 154, 157, 160, 163, 172, 543
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
PpuMI RGGWCCY 3 cut(s) 281, 328, 432
PshBI ATTAAT 1 cut(s) 104
Psp124BI GAGCTC 2 cut(s) 339, 516
Psp1406I AACGTT 1 cut(s) 503
Psp5II RGGWCCY 3 cut(s) 281, 328, 432
Psp6I CCWGG 3 cut(s) 212, 330, 369
PspEI GGTNACC 2 cut(s) 460, 713
PspGI CCWGG 3 cut(s) 212, 330, 369
PspN4I GGNNCC 4 cut(s) 282, 329, 434, 609
PspPI GGNCC 6 cut(s) 199, 281, 328, 367, 432, 558
PspPPI RGGWCCY 3 cut(s) 281, 328, 432
PsyI GACNNNGTC 1 cut(s) 425
RsaI GTAC 1 cut(s) 732
RsaNI GTAC 1 cut(s) 731
SacI GAGCTC 2 cut(s) 339, 516
SaqAI TTAA 2 cut(s) 104, 500
SatI GCNGC 6 cut(s) 153, 156, 159, 162, 171, 542
Sau3AI GATC 1 cut(s) 347
Sau96I GGNCC 6 cut(s) 199, 281, 328, 367, 432, 558
SchI GAGTC 1 cut(s) 547
ScrFI CCNGG 6 cut(s) 214, 326, 332, 371, 456, 457
SduI GDGCHC 2 cut(s) 339, 516
SfaNI GCATC 1 cut(s) 95
SinI GGWCC 5 cut(s) 199, 281, 328, 367, 432
SmaI CCCGGG 1 cut(s) 457
SmlI CTYRAG 2 cut(s) 244, 261
SmoI CTYRAG 2 cut(s) 244, 261
Sse9I AATT 2 cut(s) 497, 631
SstI GAGCTC 2 cut(s) 339, 516
StyD4I CCNGG 6 cut(s) 212, 324, 330, 369, 454, 455
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 4 cut(s) 232, 442, 607, 641
TaiI ACGT 3 cut(s) 363, 429, 506
TaqI TCGA 5 cut(s) 346, 444, 516, 522, 551
TasI AATT 2 cut(s) 497, 631
TatI WGTACW 1 cut(s) 730
TauI GCSGC 3 cut(s) 155, 158, 164
TfiI GAWTC 4 cut(s) 4, 40, 46, 548
Tru1I TTAA 2 cut(s) 104, 500
Tru9I TTAA 2 cut(s) 104, 500
TscAI CASTG 2 cut(s) 154, 181
TseFI GTSAC 2 cut(s) 460, 601
TseI GCWGC 3 cut(s) 158, 170, 541
Tsp45I GTSAC 2 cut(s) 460, 601
TspDTI ATGAA 2 cut(s) 82, 209
TspMI CCCGGG 1 cut(s) 455
TspRI CASTG 2 cut(s) 154, 181
Tth111I GACNNNGTC 1 cut(s) 425
VpaK11BI GGWCC 5 cut(s) 199, 281, 328, 367, 432
VspI ATTAAT 1 cut(s) 104
XapI RAATTY 1 cut(s) 631
XmaI CCCGGG 1 cut(s) 455
ZraI GACGTC 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.