AT4G14830

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007781
Physical Location & Seq
Forward (+)
8510678 .. 8511928
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G14830.1

Sequence Viewer

Length: 459 bp
ATGAAAATCCACCCATTACCCAGAAACGAAAATAACAACAATCTGATCCATCACGCTCGTGATCCGACCCGAGAACCCGGAAAGAAGCTCCGTCGTCTCCCACATATCTTCAGCCGTGTTCTAGAGCTGCCCTTGAAGTCAGACGCTGACGTGGCTGTTGAAGAGAGTCATGACTGCTTCAGGTTCGTCGCGGAAACAGACGGGGGAGGAGGAGGAGGTGTGAGGGCTTACATGGTTGAGATTCATCCTGGTGTGACCAAGATTCTTGTGAGAACCAATGGTTCATCGTCTCTTGGTTTGTCGCTTGATGAGCTTGAGCTTGACGTGTGGCGTTTCAGGCTACCGGAATCAACTAGGCCTGAGCTCGTTACCGTGGATTGCGACGGCGATGGAGAATTGATTGTCACTGTGCCTAAGATTGAAGACAATGGTAGAGATTTGATAGTGCTTGTACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

16.94

Weight (kDa)

5.49

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 191
AciI CCGC 1 cut(s) 191
AclWI GGATC 2 cut(s) 40, 56
AcuI CTGAAG 2 cut(s) 94, 163
AfaI GTAC 1 cut(s) 453
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 3 cut(s) 136, 161, 422
AjiI CACGTC 2 cut(s) 151, 325
AjnI CCWGG 1 cut(s) 247
AleI CACNNNNGTG 1 cut(s) 57
AluBI AGCT 5 cut(s) 88, 127, 313, 319, 364
AluI AGCT 5 cut(s) 88, 127, 313, 319, 364
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 2 cut(s) 101, 294
AlwI GGATC 2 cut(s) 40, 56
AlwNI CAGNNNCTG 1 cut(s) 146
Ama87I CYCGRG 1 cut(s) 69
AoxI GGCC 1 cut(s) 356
ApeKI GCWGC 1 cut(s) 127
AsuC2I CCSGG 1 cut(s) 78
AvaI CYCGRG 1 cut(s) 69
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 57
BbsI GAAGAC 1 cut(s) 429
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 1 cut(s) 114
BccI CCATC 2 cut(s) 57, 383
BceAI ACGGC 2 cut(s) 99, 400
BciT130I CCWGG 1 cut(s) 249
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 2 cut(s) 101, 294
BfaI CTAG 2 cut(s) 122, 354
BisI GCNGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 129
Bme1390I CCNGG 2 cut(s) 78, 249
BmeT110I CYCGRG 1 cut(s) 69
BmgBI CACGTC 2 cut(s) 151, 325
BmrFI CCNGG 2 cut(s) 78, 249
BpiI GAAGAC 1 cut(s) 429
Bpu10I CCTNAGC 1 cut(s) 360
BpuEI CTTGAG 1 cut(s) 335
BpuMI CCSGG 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 372
BsaWI WCCGGW 1 cut(s) 343
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 1 cut(s) 244
BseMII CTCAG 1 cut(s) 351
BseRI GAGGAG 3 cut(s) 222, 225, 228
BseXI GCAGC 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 191
BshFI GGCC 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 69
BsiSI CCGG 2 cut(s) 78, 344
BsmAI GTCTC 2 cut(s) 101, 294
BsmBI CGTCTC 2 cut(s) 101, 294
BsnI GGCC 1 cut(s) 358
BsoBI CYCGRG 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 366
Bsp1407I TGTACA 1 cut(s) 451
Bsp143I GATC 2 cut(s) 45, 61
BspACI CCGC 1 cut(s) 191
BspANI GGCC 1 cut(s) 358
BspCNI CTCAG 1 cut(s) 352
BspFNI CGCG 1 cut(s) 191
BspHI TCATGA 1 cut(s) 169
BspPI GGATC 2 cut(s) 40, 56
BsrGI TGTACA 1 cut(s) 451
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 2 cut(s) 45, 61
BssSI CACGAG 1 cut(s) 57
Bst2BI CACGAG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 3 cut(s) 373, 409, 456
Bst6I CTCTTC 1 cut(s) 156
BstAUI TGTACA 1 cut(s) 451
BstDEI CTNAG 2 cut(s) 360, 414
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 1 cut(s) 244
BstFNI CGCG 1 cut(s) 191
BstKTI GATC 2 cut(s) 48, 64
BstMAI GTCTC 2 cut(s) 101, 294
BstMBI GATC 2 cut(s) 45, 61
BstMWI GCNNNNNNNGC 3 cut(s) 152, 310, 337
BstNI CCWGG 1 cut(s) 249
BstSCI CCNGG 2 cut(s) 76, 247
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 114
BstV2I GAAGAC 1 cut(s) 429
BsuRI GGCC 1 cut(s) 358
BtgI CCRYGG 1 cut(s) 372
BtgZI GCGATG 1 cut(s) 402
BtrI CACGTC 2 cut(s) 151, 325
BtsCI GGATG 1 cut(s) 244
BtsIMutI CAGTG 1 cut(s) 405
CaiI CAGNNNCTG 1 cut(s) 146
CciI TCATGA 1 cut(s) 169
CseI GACGC 1 cut(s) 152
Csp6I GTAC 1 cut(s) 452
CviAII CATG 2 cut(s) 170, 232
CviQI GTAC 1 cut(s) 452
DdeI CTNAG 2 cut(s) 360, 414
DpnI GATC 2 cut(s) 47, 63
DpnII GATC 2 cut(s) 45, 61
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Ecl136II GAGCTC 1 cut(s) 364
Eco147I AGGCCT 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 366
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 2 cut(s) 94, 163
Eco88I CYCGRG 1 cut(s) 69
EcoICRI GAGCTC 1 cut(s) 364
EcoRII CCWGG 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 366
Esp3I CGTCTC 2 cut(s) 101, 294
FaeI CATG 2 cut(s) 173, 235
FaiI YATR 3 cut(s) 105, 171, 233
FatI CATG 2 cut(s) 169, 231
Fnu4HI GCNGC 1 cut(s) 128
FokI GGATG 1 cut(s) 231
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 1 cut(s) 128
FspBI CTAG 2 cut(s) 122, 354
GluI GCNGC 1 cut(s) 128
HaeIII GGCC 1 cut(s) 358
HapII CCGG 2 cut(s) 78, 344
HgaI GACGC 1 cut(s) 152
Hin1II CATG 2 cut(s) 173, 235
HinfI GANTC 4 cut(s) 166, 241, 262, 347
HpaII CCGG 2 cut(s) 78, 344
Hpy188I TCNGA 3 cut(s) 45, 66, 142
Hpy188III TCNNGA 3 cut(s) 59, 122, 170
Hpy99I CGWCG 3 cut(s) 96, 191, 386
HpyCH4III ACNGT 3 cut(s) 373, 409, 456
HpyCH4IV ACGT 2 cut(s) 150, 324
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 310, 337
HpyF3I CTNAG 2 cut(s) 360, 414
HpySE526I ACGT 2 cut(s) 150, 324
Hsp92II CATG 2 cut(s) 173, 235
Kzo9I GATC 2 cut(s) 45, 61
LmnI GCTCC 1 cut(s) 93
LpnPI CCDG 8 cut(s) 34, 91, 166, 234, 261, 322, 357, 372
Lsp1109I GCAGC 1 cut(s) 114
MaeI CTAG 2 cut(s) 122, 354
MaeII ACGT 2 cut(s) 150, 324
MaeIII GTNAC 3 cut(s) 253, 367, 403
MalI GATC 2 cut(s) 47, 63
MboI GATC 2 cut(s) 45, 61
MboII GAAGA 3 cut(s) 100, 173, 434
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 1 cut(s) 395
MlyI GAGTC 1 cut(s) 175
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 5 cut(s) 200, 203, 206, 209, 216
MslI CAYNNNNRTG 2 cut(s) 57, 249
MspI CCGG 2 cut(s) 78, 344
MspR9I CCNGG 2 cut(s) 78, 249
MvaI CCWGG 1 cut(s) 249
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 3 cut(s) 152, 310, 337
NciI CCSGG 1 cut(s) 78
NdeII GATC 2 cut(s) 45, 61
NlaIII CATG 2 cut(s) 173, 235
NmuCI GTSAC 2 cut(s) 253, 403
OliI CACNNNNGTG 1 cut(s) 57
PagI TCATGA 1 cut(s) 169
PceI AGGCCT 1 cut(s) 358
PfeI GAWTC 3 cut(s) 241, 262, 347
PkrI GCNGC 1 cut(s) 129
PleI GAGTC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 146
RsaI GTAC 1 cut(s) 453
RsaNI GTAC 1 cut(s) 452
RseI CAYNNNNRTG 2 cut(s) 57, 249
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 1 cut(s) 128
Sau3AI GATC 2 cut(s) 45, 61
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 2 cut(s) 78, 249
SduI GDGCHC 1 cut(s) 366
SetI ASST 9 cut(s) 90, 129, 153, 185, 220, 315, 321, 327, 366
SmiMI CAYNNNNRTG 2 cut(s) 57, 249
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 1 cut(s) 395
SseBI AGGCCT 1 cut(s) 358
SsiI CCGC 1 cut(s) 191
SspMI CTAG 2 cut(s) 122, 354
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 358
StyD4I CCNGG 2 cut(s) 76, 247
TaaI ACNGT 3 cut(s) 373, 409, 456
TaiI ACGT 2 cut(s) 153, 327
TasI AATT 1 cut(s) 395
TatI WGTACW 1 cut(s) 451
TfiI GAWTC 3 cut(s) 241, 262, 347
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 2 cut(s) 253, 403
TseI GCWGC 1 cut(s) 127
Tsp45I GTSAC 2 cut(s) 253, 403
TspDTI ATGAA 3 cut(s) 17, 233, 273
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 1 cut(s) 412
XbaI TCTAGA 1 cut(s) 121
XspI CTAG 2 cut(s) 122, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.