Rroxscaffold_5G00359840

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007781
Physical Location & Seq
Forward (+)
40144729 .. 40145292
564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00359840.1

Sequence Viewer

Length: 564 bp
ATGAAGGTCCATCCAATCCCCAAGAGGCGCAACATCTCCATCCAGTACCATTCAAGAAGCCCACTTTCTGAAGCCCAGGCCCTCCTCGGCCTCACCCACAAGAAGCTCCGCCGCCTCCCCCATGTCTTCAGCCGCGTCCTCGAGCTCCCTTTCCGCTCCGACGCCGACGTCCTCATCGAGGAGAACCCCGACTGCTTCCGCTTCGTCGCCGAAACCGAGAGCTTCGGCCACGACGTCAGGGCCCACACCGTCGAAATCCACCCCGGGGTGACCAAGATTGTCGTCAGGGAAAGAGGCTCCGCGGCCGAGCTGACCTTGGACGAGCTGGAGCTCGACATGTGGAGGTTCCGGCTCCCGGAGTCGACCCGGCCGGAGCTGGCCAGGGCGGTTTTTGTAGACGGGGAGCTCATTGTGACGGTGCCCAAGATGGAGGGGATGGAGAATTTGGAGGGTGGAGATGGTGGGGAGGTTTGGGGCGGCGGCGGCGGCGGCGGTGGTGGGAATGGGAATGGGAATGGGGGGTTCAGAGGAGGTATGGGAAATCGGCTTGTGCTTGTACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

20.52

Weight (kDa)

6.17

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AatII GACGTC 2 cut(s) 171, 237
AccB1I GGYRCC 1 cut(s) 418
AccBSI CCGCTC 1 cut(s) 156
AccI GTMKAC 2 cut(s) 362, 396
AccII CGCG 2 cut(s) 135, 302
AcoI YGGCCR 4 cut(s) 226, 303, 368, 378
AcsI RAATTY 1 cut(s) 442
AcuI CTGAAG 2 cut(s) 90, 112
AcyI GRCGYC 3 cut(s) 162, 168, 234
AfaI GTAC 2 cut(s) 47, 558
AfiI CCNNNNNNNGG 3 cut(s) 178, 265, 355
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 1 cut(s) 54
AjnI CCWGG 2 cut(s) 75, 380
AluBI AGCT 8 cut(s) 106, 145, 222, 310, 325, 331, 376, 406
AluI AGCT 8 cut(s) 106, 145, 222, 310, 325, 331, 376, 406
Alw21I GWGCWC 3 cut(s) 147, 333, 408
Ama87I CYCGRG 2 cut(s) 140, 263
AoxI GGCC 7 cut(s) 78, 88, 226, 240, 303, 368, 378
ApaI GGGCCC 1 cut(s) 244
ApoI RAATTY 1 cut(s) 442
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 4 cut(s) 7, 79, 240, 241
AsuC2I CCSGG 4 cut(s) 264, 265, 356, 367
AsuHPI GGTGA 2 cut(s) 85, 280
AvaI CYCGRG 2 cut(s) 140, 263
AvaII GGWCC 1 cut(s) 7
BaeGI GKGCMC 2 cut(s) 244, 423
BalI TGGCCA 1 cut(s) 380
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 4 cut(s) 147, 244, 333, 408
BbsI GAAGAC 1 cut(s) 118
Bbv12I GWGCWC 3 cut(s) 147, 333, 408
BccI CCATC 5 cut(s) 18, 47, 421, 430, 452
BciT130I CCWGG 2 cut(s) 77, 382
BcnI CCSGG 4 cut(s) 264, 265, 356, 367
BisI GCNGC 8 cut(s) 112, 133, 303, 478, 481, 484, 487, 490
BlsI GCNGC 8 cut(s) 113, 134, 304, 479, 482, 485, 488, 491
Bme1390I CCNGG 6 cut(s) 77, 264, 265, 356, 367, 382
Bme18I GGWCC 1 cut(s) 7
BmeT110I CYCGRG 2 cut(s) 140, 263
BmgT120I GGNCC 4 cut(s) 7, 79, 240, 241
BmiI GGNNCC 5 cut(s) 242, 298, 347, 353, 420
BmrFI CCNGG 6 cut(s) 77, 264, 265, 356, 367, 382
BpiI GAAGAC 1 cut(s) 118
BpmI CTGGAG 1 cut(s) 347
BpuMI CCSGG 4 cut(s) 264, 265, 356, 367
BsaHI GRCGYC 3 cut(s) 162, 168, 234
BsaJI CCNNGG 8 cut(s) 75, 85, 262, 263, 264, 300, 315, 381
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 265, 355
Bse1I ACTGG 1 cut(s) 43
BseBI CCWGG 2 cut(s) 77, 382
BseDI CCNNGG 8 cut(s) 75, 85, 262, 263, 264, 300, 315, 381
BseGI GGATG 3 cut(s) 10, 39, 441
BseLI CCNNNNNNNGG 3 cut(s) 178, 265, 355
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 3 cut(s) 74, 194, 543
BseSI GKGCMC 2 cut(s) 244, 423
BseX3I CGGCCG 2 cut(s) 303, 368
Bsh1236I CGCG 2 cut(s) 135, 302
Bsh1285I CGRYCG 2 cut(s) 306, 371
BshFI GGCC 7 cut(s) 80, 90, 228, 242, 305, 370, 380
BshNI GGYRCC 1 cut(s) 418
BsiEI CGRYCG 2 cut(s) 306, 371
BsiHKAI GWGCWC 3 cut(s) 147, 333, 408
BsiHKCI CYCGRG 2 cut(s) 140, 263
BsiSI CCGG 5 cut(s) 264, 349, 356, 367, 371
BslI CCNNNNNNNGG 3 cut(s) 178, 265, 355
BsnI GGCC 7 cut(s) 80, 90, 228, 242, 305, 370, 380
BsoBI CYCGRG 2 cut(s) 140, 263
Bsp120I GGGCCC 1 cut(s) 240
Bsp1286I GDGCHC 5 cut(s) 147, 244, 333, 408, 423
Bsp1407I TGTACA 1 cut(s) 556
BspANI GGCC 7 cut(s) 80, 90, 228, 242, 305, 370, 380
BspFNI CGCG 2 cut(s) 135, 302
BspLI GGNNCC 5 cut(s) 242, 298, 347, 353, 420
BspT107I GGYRCC 1 cut(s) 418
BsrBI CCGCTC 1 cut(s) 156
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 1 cut(s) 43
BssECI CCNNGG 8 cut(s) 75, 85, 262, 263, 264, 300, 315, 381
BssNI GRCGYC 3 cut(s) 162, 168, 234
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 77, 382
Bst4CI ACNGT 3 cut(s) 250, 418, 561
BstACI GRCGYC 3 cut(s) 162, 168, 234
BstAUI TGTACA 1 cut(s) 556
BstC8I GCNNGC 1 cut(s) 378
BstDSI CCRYGG 1 cut(s) 300
BstEII GGTNACC 1 cut(s) 268
BstENI CCTNNNNNAGG 1 cut(s) 176
BstF5I GGATG 3 cut(s) 10, 39, 441
BstFNI CGCG 2 cut(s) 135, 302
BstHHI GCGC 1 cut(s) 30
BstMCI CGRYCG 2 cut(s) 306, 371
BstMWI GCNNNNNNNGC 3 cut(s) 483, 486, 489
BstNI CCWGG 2 cut(s) 77, 382
BstNSI RCATGY 1 cut(s) 340
BstPI GGTNACC 1 cut(s) 268
BstSCI CCNGG 6 cut(s) 75, 262, 263, 354, 365, 380
BstSLI GKGCMC 2 cut(s) 244, 423
BstUI CGCG 2 cut(s) 135, 302
BstV2I GAAGAC 1 cut(s) 118
BstZI CGGCCG 2 cut(s) 303, 368
BsuRI GGCC 7 cut(s) 80, 90, 228, 242, 305, 370, 380
BtgI CCRYGG 1 cut(s) 300
BtsCI GGATG 3 cut(s) 10, 39, 441
Cac8I GCNNGC 1 cut(s) 378
CfoI GCGC 1 cut(s) 30
Cfr13I GGNCC 4 cut(s) 7, 79, 240, 241
Cfr42I CCGCGG 1 cut(s) 303
Cfr9I CCCGGG 1 cut(s) 263
CseI GACGC 2 cut(s) 124, 170
Csp6I GTAC 2 cut(s) 46, 557
CviAII CATG 2 cut(s) 122, 337
CviQI GTAC 2 cut(s) 46, 557
DrdI GACNNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 4 cut(s) 226, 303, 368, 378
EagI CGGCCG 2 cut(s) 303, 368
EciI GGCGGA 1 cut(s) 98
Ecl136II GAGCTC 3 cut(s) 145, 331, 406
EclXI CGGCCG 2 cut(s) 303, 368
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 4 cut(s) 147, 244, 333, 408
Eco47I GGWCC 1 cut(s) 7
Eco52I CGGCCG 2 cut(s) 303, 368
Eco53kI GAGCTC 3 cut(s) 145, 331, 406
Eco57I CTGAAG 2 cut(s) 90, 112
Eco88I CYCGRG 2 cut(s) 140, 263
Eco91I GGTNACC 1 cut(s) 268
EcoICRI GAGCTC 3 cut(s) 145, 331, 406
EcoNI CCTNNNNNAGG 1 cut(s) 176
EcoO109I RGGNCCY 2 cut(s) 79, 240
EcoO65I GGTNACC 1 cut(s) 268
EcoRII CCWGG 2 cut(s) 75, 380
EcoT14I CCWWGG 1 cut(s) 315
EcoT38I GRGCYC 4 cut(s) 147, 244, 333, 408
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 2 cut(s) 125, 340
FaiI YATR 3 cut(s) 123, 338, 536
FatI CATG 2 cut(s) 121, 336
FblI GTMKAC 2 cut(s) 362, 396
Fnu4HI GCNGC 8 cut(s) 112, 133, 303, 478, 481, 484, 487, 490
FokI GGATG 2 cut(s) 26, 448
FriOI GRGCYC 4 cut(s) 147, 244, 333, 408
Fsp4HI GCNGC 8 cut(s) 112, 133, 303, 478, 481, 484, 487, 490
GlaI GCGC 1 cut(s) 29
GluI GCNGC 8 cut(s) 112, 133, 303, 478, 481, 484, 487, 490
GsuI CTGGAG 1 cut(s) 347
HaeIII GGCC 7 cut(s) 80, 90, 228, 242, 305, 370, 380
HapII CCGG 5 cut(s) 264, 349, 356, 367, 371
HgaI GACGC 2 cut(s) 124, 170
HhaI GCGC 1 cut(s) 30
Hin1I GRCGYC 3 cut(s) 162, 168, 234
Hin1II CATG 2 cut(s) 125, 340
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
HinfI GANTC 1 cut(s) 359
HpaII CCGG 5 cut(s) 264, 349, 356, 367, 371
HphI GGTGA 2 cut(s) 85, 280
Hpy166II GTNNAC 2 cut(s) 363, 397
Hpy188I TCNGA 3 cut(s) 70, 160, 527
Hpy188III TCNNGA 1 cut(s) 54
Hpy8I GTNNAC 2 cut(s) 363, 397
Hpy99I CGWCG 5 cut(s) 164, 170, 209, 236, 254
HpyCH4III ACNGT 3 cut(s) 250, 418, 561
HpyCH4IV ACGT 2 cut(s) 168, 234
HpyF10VI GCNNNNNNNGC 3 cut(s) 483, 486, 489
HpySE526I ACGT 2 cut(s) 168, 234
Hsp92I GRCGYC 3 cut(s) 162, 168, 234
Hsp92II CATG 2 cut(s) 125, 340
HspAI GCGC 1 cut(s) 28
KspI CCGCGG 1 cut(s) 303
LmnI GCTCC 8 cut(s) 111, 150, 161, 302, 328, 357, 373, 403
MaeII ACGT 2 cut(s) 168, 234
MaeIII GTNAC 2 cut(s) 268, 412
MbiI CCGCTC 1 cut(s) 156
MboII GAAGA 1 cut(s) 118
MhlI GDGCHC 5 cut(s) 147, 244, 333, 408, 423
MlsI TGGCCA 1 cut(s) 380
MluCI AATT 1 cut(s) 442
MluNI TGGCCA 1 cut(s) 380
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 183
Mox20I TGGCCA 1 cut(s) 380
MscI TGGCCA 1 cut(s) 380
Msp20I TGGCCA 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 5 cut(s) 264, 349, 356, 367, 371
MspR9I CCNGG 6 cut(s) 77, 264, 265, 356, 367, 382
MvaI CCWGG 2 cut(s) 77, 382
MvnI CGCG 2 cut(s) 135, 302
MwoI GCNNNNNNNGC 3 cut(s) 483, 486, 489
NciI CCSGG 4 cut(s) 264, 265, 356, 367
NlaIII CATG 2 cut(s) 125, 340
NlaIV GGNNCC 5 cut(s) 242, 298, 347, 353, 420
NmeAIII GCCGAG 2 cut(s) 66, 331
NmuCI GTSAC 2 cut(s) 268, 412
NspI RCATGY 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 140
PciI ACATGT 1 cut(s) 336
PcsI WCGNNNNNNNCGW 3 cut(s) 174, 213, 231
PfoI TCCNGGA 1 cut(s) 354
PkrI GCNGC 8 cut(s) 113, 134, 304, 479, 482, 485, 488, 491
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
PscI ACATGT 1 cut(s) 336
Psp124BI GAGCTC 3 cut(s) 147, 333, 408
Psp6I CCWGG 2 cut(s) 75, 380
PspEI GGTNACC 1 cut(s) 268
PspGI CCWGG 2 cut(s) 75, 380
PspN4I GGNNCC 5 cut(s) 242, 298, 347, 353, 420
PspOMI GGGCCC 1 cut(s) 240
PspPI GGNCC 4 cut(s) 7, 79, 240, 241
PspXI VCTCGAGB 1 cut(s) 140
RsaI GTAC 2 cut(s) 47, 558
RsaNI GTAC 2 cut(s) 46, 557
SacI GAGCTC 3 cut(s) 147, 333, 408
SacII CCGCGG 1 cut(s) 303
SalI GTCGAC 1 cut(s) 361
SatI GCNGC 8 cut(s) 112, 133, 303, 478, 481, 484, 487, 490
Sau96I GGNCC 4 cut(s) 7, 79, 240, 241
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 6 cut(s) 77, 264, 265, 356, 367, 382
SduI GDGCHC 5 cut(s) 147, 244, 333, 408, 423
Sfr274I CTCGAG 1 cut(s) 140
Sfr303I CCGCGG 1 cut(s) 303
SgrBI CCGCGG 1 cut(s) 303
SinI GGWCC 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 140
SmaI CCCGGG 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 1 cut(s) 442
SstI GAGCTC 3 cut(s) 147, 333, 408
StyD4I CCNGG 6 cut(s) 75, 262, 263, 354, 365, 380
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 3 cut(s) 250, 418, 561
TaiI ACGT 2 cut(s) 171, 237
TaqI TCGA 5 cut(s) 141, 177, 252, 333, 362
TasI AATT 1 cut(s) 442
TatI WGTACW 1 cut(s) 556
TauI GCSGC 8 cut(s) 114, 135, 305, 480, 483, 486, 489, 492
TseFI GTSAC 2 cut(s) 268, 412
Tsp45I GTSAC 2 cut(s) 268, 412
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 7
XagI CCTNNNNNAGG 1 cut(s) 176
XapI RAATTY 1 cut(s) 442
XceI RCATGY 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 140
XmaI CCCGGG 1 cut(s) 263
XmiI GTMKAC 2 cut(s) 362, 396
ZraI GACGTC 2 cut(s) 169, 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.