MD16G1195400.v1.1

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007781
Physical Location & Seq
Reverse (-)
17389845 .. 17391884
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1195400.v1.1.491

Sequence Viewer

Length: 780 bp
ATGATTCGTCCTTTTGGTAAAAACAACCACCCAATCAGGATTCTGATTCCCACCCAAGGTTGGACTGCCTTTTCCAGAAGAAGGGCATCTTTCATCTTTCTATTAATCTTGTGCCAATCCCTCTATTTCTTCTGCACAACCACCACCACTGCCTCCGCCGCCGCCGTAGCTGCCACTGAAACAGCCCAAACCATGAAGGTCCACCCCATGCCTAGGAAGCGCAACATCACCGTCCAATACAGCTCAAGAAACTCACTTTCTGAAGCGCAGGCACTTTTGGGGCCGAACCACAAGAAGCTCCGCCGTCTCCCCCATGTCTTCAGCCGGGTCCTGGAGCTCCCCTTTCGATCCGACGCCGACGTTCTGGTCCAGGAGAACCCCGATTGCTTCCGATTTGTCGCCGAAGCCGATAATATCAGCGACGACGTCAGGGCCCATACCGTCGAAATCCATCCCGGGGTGACCAAAATCGTCGTTCGGGAAAGTGGGTCGGTGGAATTAACGTTGGACGAGCTGGAACTCGATATGTGGAGGTTTAGGCTGCCGGAATCGACTCGGCCCGAGCTGGCCAGCGCGGTTTTTGTTGGCGGGGAGCTTATCGTGACGGTGCCGAAGGCCGAGGGGTTGGGGAATTCGGACGGTGGTGATGGCGGCGGTGGAGACGGGAATGGGGGCGGGGGTTTCCGAGGAGGTACTGCCTTTATTCCATTACCAAGAGCCAGGCAATTACTCGATGGTTATAAAATGAACATCTCTCTGCTTTTCTTAGTTCTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

28.43

Weight (kDa)

8.86

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 741
AasI GACNNNNNNGTC 1 cut(s) 365
AatII GACGTC 1 cut(s) 429
AccB1I GGYRCC 1 cut(s) 607
AccII CGCG 1 cut(s) 575
AciI CCGC 9 cut(s) 156, 159, 162, 301, 575, 588, 651, 654, 675
AclI AACGTT 1 cut(s) 503
AclWI GGATC 1 cut(s) 342
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 1 cut(s) 631
AcuI CTGAAG 2 cut(s) 282, 304
AcyI GRCGYC 2 cut(s) 354, 426
AfaI GTAC 1 cut(s) 694
AfiI CCNNNNNNNGG 6 cut(s) 56, 60, 81, 213, 331, 457
AjnI CCWGG 3 cut(s) 330, 369, 719
AluBI AGCT 7 cut(s) 170, 243, 298, 337, 514, 565, 595
AluI AGCT 7 cut(s) 170, 243, 298, 337, 514, 565, 595
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 311, 654
AlwI GGATC 1 cut(s) 342
Ama87I CYCGRG 2 cut(s) 455, 560
AoxI GGCC 5 cut(s) 281, 432, 557, 567, 615
ApaI GGGCCC 1 cut(s) 436
ApeKI GCWGC 2 cut(s) 170, 541
ApoI RAATTY 1 cut(s) 631
AseI ATTAAT 1 cut(s) 104
AspA2I CCTAGG 1 cut(s) 212
AspLEI GCGC 3 cut(s) 222, 268, 575
AspS9I GGNCC 7 cut(s) 199, 281, 328, 367, 432, 433, 558
AsuC2I CCSGG 3 cut(s) 326, 456, 457
AsuHPI GGTGA 3 cut(s) 220, 472, 656
AvaI CYCGRG 2 cut(s) 455, 560
AvaII GGWCC 3 cut(s) 199, 328, 367
AvrII CCTAGG 1 cut(s) 212
BaeGI GKGCMC 1 cut(s) 436
BalI TGGCCA 1 cut(s) 569
BanI GGYRCC 1 cut(s) 607
BanII GRGCYC 2 cut(s) 339, 436
BbsI GAAGAC 1 cut(s) 310
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 2 cut(s) 157, 528
BccI CCATC 3 cut(s) 459, 641, 728
BceAI ACGGC 2 cut(s) 149, 288
BciT130I CCWGG 3 cut(s) 332, 371, 721
BcnI CCSGG 3 cut(s) 326, 456, 457
BcoDI GTCTC 2 cut(s) 311, 654
BfaI CTAG 1 cut(s) 213
BisI GCNGC 5 cut(s) 159, 162, 171, 542, 652
BlnI CCTAGG 1 cut(s) 212
BlsI GCNGC 5 cut(s) 160, 163, 172, 543, 653
Bme1390I CCNGG 6 cut(s) 326, 332, 371, 456, 457, 721
Bme18I GGWCC 3 cut(s) 199, 328, 367
BmeT110I CYCGRG 2 cut(s) 455, 560
BmgT120I GGNCC 7 cut(s) 199, 281, 328, 367, 432, 433, 558
BmiI GGNNCC 4 cut(s) 282, 329, 434, 609
BmrFI CCNGG 6 cut(s) 326, 332, 371, 456, 457, 721
BmsI GCATC 1 cut(s) 95
BpiI GAAGAC 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 353
BpuEI CTTGAG 1 cut(s) 229
BpuMI CCSGG 3 cut(s) 326, 456, 457
BsaHI GRCGYC 2 cut(s) 354, 426
BsaJI CCNNGG 6 cut(s) 55, 212, 455, 456, 618, 685
Bsc4I CCNNNNNNNGG 6 cut(s) 56, 60, 81, 213, 331, 457
BseBI CCWGG 3 cut(s) 332, 371, 721
BseDI CCNNGG 6 cut(s) 55, 212, 455, 456, 618, 685
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 6 cut(s) 56, 60, 81, 213, 331, 457
BseRI GAGGAG 1 cut(s) 702
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 2 cut(s) 157, 528
BsgI GTGCAG 1 cut(s) 118
Bsh1236I CGCG 1 cut(s) 575
BshFI GGCC 5 cut(s) 283, 434, 559, 569, 617
BshNI GGYRCC 1 cut(s) 607
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 2 cut(s) 455, 560
BsiSI CCGG 3 cut(s) 325, 456, 545
BslI CCNNNNNNNGG 6 cut(s) 56, 60, 81, 213, 331, 457
BsmAI GTCTC 2 cut(s) 311, 654
BsmBI CGTCTC 2 cut(s) 311, 654
BsnI GGCC 5 cut(s) 283, 434, 559, 569, 617
BsoBI CYCGRG 2 cut(s) 455, 560
Bsp120I GGGCCC 1 cut(s) 432
Bsp1286I GDGCHC 2 cut(s) 339, 436
Bsp143I GATC 1 cut(s) 347
BspACI CCGC 9 cut(s) 156, 159, 162, 301, 575, 588, 651, 654, 675
BspANI GGCC 5 cut(s) 283, 434, 559, 569, 617
BspFNI CGCG 1 cut(s) 575
BspLI GGNNCC 4 cut(s) 282, 329, 434, 609
BspPI GGATC 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 607
BssECI CCNNGG 6 cut(s) 55, 212, 455, 456, 618, 685
BssMI GATC 1 cut(s) 347
BssNI GRCGYC 2 cut(s) 354, 426
BssT1I CCWWGG 2 cut(s) 55, 212
Bst2UI CCWGG 3 cut(s) 332, 371, 721
Bst4CI ACNGT 4 cut(s) 232, 442, 607, 641
BstACI GRCGYC 2 cut(s) 354, 426
BstC8I GCNNGC 3 cut(s) 270, 567, 571
BstDEI CTNAG 1 cut(s) 766
BstEII GGTNACC 1 cut(s) 460
BstF5I GGATG 1 cut(s) 451
BstFNI CGCG 1 cut(s) 575
BstHHI GCGC 3 cut(s) 222, 268, 575
BstKTI GATC 1 cut(s) 350
BstMAI GTCTC 2 cut(s) 311, 654
BstMBI GATC 1 cut(s) 347
BstMWI GCNNNNNNNGC 4 cut(s) 158, 167, 170, 217
BstNI CCWGG 3 cut(s) 332, 371, 721
BstPI GGTNACC 1 cut(s) 460
BstSCI CCNGG 6 cut(s) 324, 330, 369, 454, 455, 719
BstSLI GKGCMC 1 cut(s) 436
BstUI CGCG 1 cut(s) 575
BstV1I GCAGC 2 cut(s) 157, 528
BstV2I GAAGAC 1 cut(s) 310
BsuRI GGCC 5 cut(s) 283, 434, 559, 569, 617
BtsCI GGATG 1 cut(s) 451
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 174
Cac8I GCNNGC 3 cut(s) 270, 567, 571
CfoI GCGC 3 cut(s) 222, 268, 575
Cfr13I GGNCC 7 cut(s) 199, 281, 328, 367, 432, 433, 558
Cfr9I CCCGGG 1 cut(s) 455
CseI GACGC 1 cut(s) 362
Csp6I GTAC 1 cut(s) 693
CviAII CATG 3 cut(s) 193, 208, 314
CviQI GTAC 1 cut(s) 693
DdeI CTNAG 1 cut(s) 766
DpnI GATC 1 cut(s) 349
DpnII GATC 1 cut(s) 347
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
EaeI YGGCCR 1 cut(s) 567
EciI GGCGGA 2 cut(s) 145, 290
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 2 cut(s) 55, 212
Eco24I GRGCYC 2 cut(s) 339, 436
Eco47I GGWCC 3 cut(s) 199, 328, 367
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 2 cut(s) 282, 304
Eco88I CYCGRG 2 cut(s) 455, 560
Eco91I GGTNACC 1 cut(s) 460
EcoICRI GAGCTC 1 cut(s) 337
EcoO109I RGGNCCY 2 cut(s) 328, 432
EcoO65I GGTNACC 1 cut(s) 460
EcoRI GAATTC 1 cut(s) 631
EcoRII CCWGG 3 cut(s) 330, 369, 719
EcoT14I CCWWGG 2 cut(s) 55, 212
EcoT38I GRGCYC 2 cut(s) 339, 436
ErhI CCWWGG 2 cut(s) 55, 212
Esp3I CGTCTC 2 cut(s) 311, 654
FaeI CATG 3 cut(s) 196, 211, 317
FaiI YATR 6 cut(s) 194, 209, 315, 438, 527, 741
FalI AAGNNNNNCTT 2 cut(s) 73, 105
FatI CATG 3 cut(s) 192, 207, 313
FauI CCCGC 2 cut(s) 581, 668
Fnu4HI GCNGC 5 cut(s) 159, 162, 171, 542, 652
FokI GGATG 1 cut(s) 438
FriOI GRGCYC 2 cut(s) 339, 436
Fsp4HI GCNGC 5 cut(s) 159, 162, 171, 542, 652
FspBI CTAG 1 cut(s) 213
GlaI GCGC 3 cut(s) 221, 267, 574
GluI GCNGC 5 cut(s) 159, 162, 171, 542, 652
GsuI CTGGAG 1 cut(s) 353
HaeIII GGCC 5 cut(s) 283, 434, 559, 569, 617
HapII CCGG 3 cut(s) 325, 456, 545
HgaI GACGC 1 cut(s) 362
HhaI GCGC 3 cut(s) 222, 268, 575
Hin1I GRCGYC 2 cut(s) 354, 426
Hin1II CATG 3 cut(s) 196, 211, 317
Hin6I GCGC 3 cut(s) 220, 266, 573
HinP1I GCGC 3 cut(s) 220, 266, 573
HinfI GANTC 5 cut(s) 4, 40, 46, 548, 553
HpaII CCGG 3 cut(s) 325, 456, 545
HphI GGTGA 3 cut(s) 220, 472, 656
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 7 cut(s) 45, 262, 352, 392, 637, 686, 779
Hpy188III TCNNGA 5 cut(s) 37, 75, 246, 479, 601
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 6 cut(s) 356, 362, 425, 428, 446, 476
HpyAV CCTTC 3 cut(s) 75, 190, 607
HpyCH4III ACNGT 4 cut(s) 232, 442, 607, 641
HpyCH4IV ACGT 3 cut(s) 360, 426, 503
HpyCH4V TGCA 1 cut(s) 135
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 167, 170, 217
HpyF3I CTNAG 1 cut(s) 766
HpySE526I ACGT 3 cut(s) 360, 426, 503
Hsp92I GRCGYC 2 cut(s) 354, 426
Hsp92II CATG 3 cut(s) 196, 211, 317
HspAI GCGC 3 cut(s) 220, 266, 573
Kzo9I GATC 1 cut(s) 347
LmnI GCTCC 4 cut(s) 303, 334, 342, 592
Lsp1109I GCAGC 2 cut(s) 157, 528
LweI GCATC 1 cut(s) 95
MaeI CTAG 1 cut(s) 213
MaeII ACGT 3 cut(s) 360, 426, 503
MaeIII GTNAC 2 cut(s) 460, 601
MalI GATC 1 cut(s) 349
MboI GATC 1 cut(s) 347
MboII GAAGA 4 cut(s) 90, 121, 310, 764
MhlI GDGCHC 2 cut(s) 339, 436
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 3 cut(s) 497, 631, 725
MluNI TGGCCA 1 cut(s) 569
MlyI GAGTC 1 cut(s) 547
MmeI TCCRAC 3 cut(s) 41, 375, 486
MnlI CCTC 6 cut(s) 131, 163, 525, 613, 680, 683
Mox20I TGGCCA 1 cut(s) 569
MscI TGGCCA 1 cut(s) 569
MseI TTAA 2 cut(s) 104, 500
Msp20I TGGCCA 1 cut(s) 569
MspI CCGG 3 cut(s) 325, 456, 545
MspR9I CCNGG 6 cut(s) 326, 332, 371, 456, 457, 721
MvaI CCWGG 3 cut(s) 332, 371, 721
MvnI CGCG 1 cut(s) 575
MwoI GCNNNNNNNGC 4 cut(s) 158, 167, 170, 217
NciI CCSGG 3 cut(s) 326, 456, 457
NdeII GATC 1 cut(s) 347
NlaIII CATG 3 cut(s) 196, 211, 317
NlaIV GGNNCC 4 cut(s) 282, 329, 434, 609
NmeAIII GCCGAG 2 cut(s) 535, 643
NmuCI GTSAC 2 cut(s) 460, 601
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PfeI GAWTC 4 cut(s) 4, 40, 46, 548
PflFI GACNNNGTC 1 cut(s) 425
PfoI TCCNGGA 2 cut(s) 330, 369
PkrI GCNGC 5 cut(s) 160, 163, 172, 543, 653
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
PpuMI RGGWCCY 1 cut(s) 328
PshBI ATTAAT 1 cut(s) 104
PsiI TTATAA 1 cut(s) 741
Psp124BI GAGCTC 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 503
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 3 cut(s) 330, 369, 719
PspEI GGTNACC 1 cut(s) 460
PspGI CCWGG 3 cut(s) 330, 369, 719
PspN4I GGNNCC 4 cut(s) 282, 329, 434, 609
PspOMI GGGCCC 1 cut(s) 432
PspPI GGNCC 7 cut(s) 199, 281, 328, 367, 432, 433, 558
PspPPI RGGWCCY 1 cut(s) 328
PsyI GACNNNGTC 1 cut(s) 425
RsaI GTAC 1 cut(s) 694
RsaNI GTAC 1 cut(s) 693
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 2 cut(s) 104, 500
SatI GCNGC 5 cut(s) 159, 162, 171, 542, 652
Sau3AI GATC 1 cut(s) 347
Sau96I GGNCC 7 cut(s) 199, 281, 328, 367, 432, 433, 558
SchI GAGTC 1 cut(s) 547
ScrFI CCNGG 6 cut(s) 326, 332, 371, 456, 457, 721
SduI GDGCHC 2 cut(s) 339, 436
SfaNI GCATC 1 cut(s) 95
SinI GGWCC 3 cut(s) 199, 328, 367
SmaI CCCGGG 1 cut(s) 457
SmlI CTYRAG 1 cut(s) 244
SmoI CTYRAG 1 cut(s) 244
Sse9I AATT 3 cut(s) 497, 631, 725
SsiI CCGC 9 cut(s) 156, 159, 162, 301, 575, 588, 651, 654, 675
SspMI CTAG 1 cut(s) 213
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 6 cut(s) 324, 330, 369, 454, 455, 719
StyI CCWWGG 2 cut(s) 55, 212
TaaI ACNGT 4 cut(s) 232, 442, 607, 641
TaiI ACGT 3 cut(s) 363, 429, 506
TaqI TCGA 5 cut(s) 346, 444, 522, 551, 732
TasI AATT 3 cut(s) 497, 631, 725
TauI GCSGC 3 cut(s) 161, 164, 654
TfiI GAWTC 4 cut(s) 4, 40, 46, 548
Tru1I TTAA 2 cut(s) 104, 500
Tru9I TTAA 2 cut(s) 104, 500
TscAI CASTG 2 cut(s) 154, 181
TseFI GTSAC 2 cut(s) 460, 601
TseI GCWGC 2 cut(s) 170, 541
Tsp45I GTSAC 2 cut(s) 460, 601
TspDTI ATGAA 3 cut(s) 82, 209, 761
TspMI CCCGGG 1 cut(s) 455
TspRI CASTG 2 cut(s) 154, 181
Tth111I GACNNNGTC 1 cut(s) 425
VpaK11BI GGWCC 3 cut(s) 199, 328, 367
VspI ATTAAT 1 cut(s) 104
XapI RAATTY 1 cut(s) 631
XmaI CCCGGG 1 cut(s) 455
XmaJI CCTAGG 1 cut(s) 212
XspI CTAG 1 cut(s) 213
ZraI GACGTC 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.