Rh5BG016200

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007781
Physical Location & Seq
Forward (+)
1150357 .. 1150930
574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG016200.1

Sequence Viewer

Length: 540 bp
ATGAAGGTCCATCCAATCCCCAAGAGGCGCAACATCTCCATCCAGTACCATTCAAGAAGCCCACTCTCTGAAGCCCAGGCCCTCCTCGGTCTCACCCACAAGAAGCTCCGCCGCCTCCCCCATGTCTTCAGCCGCGTCCTCGAGCTCCCTTTCCGCTCCGACGCCGACGTCCTCATCGAGGAGAACCCCGACTGCTTCCGCTTCGTCGCCGAAACCGAGAGCTTCGGCCACGACGTCAGGGCCCACACCGTCGAAATCCACCCCGGGGTGACCAAGATTGTCGTCAGGGAAAGAGGGTCCGCGGCGGAGCTGACGTTGGACGAGCTGGAGCTGGACATGTGGAGGTTCCGCCTACCGGAGTCGACCCGGACGGAGCTGGCCAGGGCGGTTTTTGTCGACGGGGAGCTCATTGTGACGGTGCCGAAGATGGAGGGGATGGAGAATTCGGAGGGCGGAGATGGTGGGGAGGTTTGGGGCGGTGGTGGTGGGAATGGGAATGGGGGTTCAGAGGAGCTGCAAATCTGGGTTTTCAGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

20.1

Weight (kDa)

5.67

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AatII GACGTC 2 cut(s) 171, 237
AccB1I GGYRCC 1 cut(s) 418
AccBSI CCGCTC 1 cut(s) 156
AccI GTMKAC 2 cut(s) 362, 396
AccII CGCG 2 cut(s) 135, 302
AcoI YGGCCR 2 cut(s) 226, 378
AcsI RAATTY 1 cut(s) 442
AcuI CTGAAG 2 cut(s) 90, 112
AcyI GRCGYC 3 cut(s) 162, 168, 234
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 3 cut(s) 178, 265, 355
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 1 cut(s) 54
AjnI CCWGG 2 cut(s) 75, 380
AloI GAACNNNNNNTCC 2 cut(s) 487, 519
AluBI AGCT 9 cut(s) 106, 145, 222, 310, 325, 331, 376, 406, 514
AluI AGCT 9 cut(s) 106, 145, 222, 310, 325, 331, 376, 406, 514
Alw21I GWGCWC 2 cut(s) 147, 408
Alw26I GTCTC 1 cut(s) 95
Ama87I CYCGRG 2 cut(s) 140, 263
AoxI GGCC 4 cut(s) 78, 226, 240, 378
ApaI GGGCCC 1 cut(s) 244
ApeKI GCWGC 1 cut(s) 514
ApoI RAATTY 1 cut(s) 442
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 5 cut(s) 7, 79, 240, 241, 297
AsuC2I CCSGG 3 cut(s) 264, 265, 367
AsuHPI GGTGA 2 cut(s) 85, 280
AvaI CYCGRG 2 cut(s) 140, 263
AvaII GGWCC 2 cut(s) 7, 297
BaeGI GKGCMC 1 cut(s) 244
BalI TGGCCA 1 cut(s) 380
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 3 cut(s) 147, 244, 408
BbsI GAAGAC 1 cut(s) 118
Bbv12I GWGCWC 2 cut(s) 147, 408
BbvI GCAGC 1 cut(s) 501
BccI CCATC 5 cut(s) 18, 47, 421, 430, 452
BciT130I CCWGG 2 cut(s) 77, 382
BcnI CCSGG 3 cut(s) 264, 265, 367
BcoDI GTCTC 1 cut(s) 95
BisI GCNGC 4 cut(s) 112, 133, 303, 515
BlsI GCNGC 4 cut(s) 113, 134, 304, 516
Bme1390I CCNGG 5 cut(s) 77, 264, 265, 367, 382
Bme18I GGWCC 2 cut(s) 7, 297
BmeT110I CYCGRG 2 cut(s) 140, 263
BmgT120I GGNCC 5 cut(s) 7, 79, 240, 241, 297
BmiI GGNNCC 4 cut(s) 242, 298, 347, 420
BmrFI CCNGG 5 cut(s) 77, 264, 265, 367, 382
BpiI GAAGAC 1 cut(s) 118
BpmI CTGGAG 1 cut(s) 347
BpuMI CCSGG 3 cut(s) 264, 265, 367
BsaHI GRCGYC 3 cut(s) 162, 168, 234
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 7 cut(s) 75, 85, 262, 263, 264, 300, 381
BsaWI WCCGGW 1 cut(s) 355
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 265, 355
Bse1I ACTGG 1 cut(s) 43
BseBI CCWGG 2 cut(s) 77, 382
BseDI CCNNGG 7 cut(s) 75, 85, 262, 263, 264, 300, 381
BseGI GGATG 3 cut(s) 10, 39, 441
BseLI CCNNNNNNNGG 3 cut(s) 178, 265, 355
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 3 cut(s) 74, 194, 524
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 1 cut(s) 501
Bsh1236I CGCG 2 cut(s) 135, 302
BshFI GGCC 4 cut(s) 80, 228, 242, 380
BshNI GGYRCC 1 cut(s) 418
BsiHKAI GWGCWC 2 cut(s) 147, 408
BsiHKCI CYCGRG 2 cut(s) 140, 263
BsiSI CCGG 3 cut(s) 264, 356, 367
BslI CCNNNNNNNGG 3 cut(s) 178, 265, 355
BsmAI GTCTC 1 cut(s) 95
BsnI GGCC 4 cut(s) 80, 228, 242, 380
Bso31I GGTCTC 1 cut(s) 95
BsoBI CYCGRG 2 cut(s) 140, 263
Bsp120I GGGCCC 1 cut(s) 240
Bsp1286I GDGCHC 3 cut(s) 147, 244, 408
BspANI GGCC 4 cut(s) 80, 228, 242, 380
BspFNI CGCG 2 cut(s) 135, 302
BspLI GGNNCC 4 cut(s) 242, 298, 347, 420
BspT107I GGYRCC 1 cut(s) 418
BspTNI GGTCTC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 156
BsrI ACTGG 1 cut(s) 43
BssECI CCNNGG 7 cut(s) 75, 85, 262, 263, 264, 300, 381
BssNI GRCGYC 3 cut(s) 162, 168, 234
Bst2UI CCWGG 2 cut(s) 77, 382
Bst4CI ACNGT 2 cut(s) 250, 418
BstACI GRCGYC 3 cut(s) 162, 168, 234
BstC8I GCNNGC 1 cut(s) 378
BstDSI CCRYGG 1 cut(s) 300
BstEII GGTNACC 1 cut(s) 268
BstENI CCTNNNNNAGG 1 cut(s) 176
BstF5I GGATG 3 cut(s) 10, 39, 441
BstFNI CGCG 2 cut(s) 135, 302
BstHHI GCGC 1 cut(s) 30
BstMAI GTCTC 1 cut(s) 95
BstNI CCWGG 2 cut(s) 77, 382
BstNSI RCATGY 1 cut(s) 340
BstPI GGTNACC 1 cut(s) 268
BstSCI CCNGG 5 cut(s) 75, 262, 263, 365, 380
BstSLI GKGCMC 1 cut(s) 244
BstUI CGCG 2 cut(s) 135, 302
BstV1I GCAGC 1 cut(s) 501
BstV2I GAAGAC 1 cut(s) 118
BsuRI GGCC 4 cut(s) 80, 228, 242, 380
BtgI CCRYGG 1 cut(s) 300
BtsCI GGATG 3 cut(s) 10, 39, 441
Cac8I GCNNGC 1 cut(s) 378
CfoI GCGC 1 cut(s) 30
Cfr13I GGNCC 5 cut(s) 7, 79, 240, 241, 297
Cfr42I CCGCGG 1 cut(s) 303
Cfr9I CCCGGG 1 cut(s) 263
CseI GACGC 2 cut(s) 124, 170
Csp6I GTAC 1 cut(s) 46
CviAII CATG 2 cut(s) 122, 337
CviQI GTAC 1 cut(s) 46
DrdI GACNNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 2 cut(s) 226, 378
EciI GGCGGA 4 cut(s) 98, 320, 338, 468
Ecl136II GAGCTC 2 cut(s) 145, 406
Eco24I GRGCYC 3 cut(s) 147, 244, 408
Eco31I GGTCTC 1 cut(s) 95
Eco47I GGWCC 2 cut(s) 7, 297
Eco53kI GAGCTC 2 cut(s) 145, 406
Eco57I CTGAAG 2 cut(s) 90, 112
Eco88I CYCGRG 2 cut(s) 140, 263
Eco91I GGTNACC 1 cut(s) 268
EcoICRI GAGCTC 2 cut(s) 145, 406
EcoNI CCTNNNNNAGG 1 cut(s) 176
EcoO109I RGGNCCY 2 cut(s) 79, 240
EcoO65I GGTNACC 1 cut(s) 268
EcoRI GAATTC 1 cut(s) 442
EcoRII CCWGG 2 cut(s) 75, 380
EcoT38I GRGCYC 3 cut(s) 147, 244, 408
FaeI CATG 2 cut(s) 125, 340
FaiI YATR 2 cut(s) 123, 338
FatI CATG 2 cut(s) 121, 336
FblI GTMKAC 2 cut(s) 362, 396
Fnu4HI GCNGC 4 cut(s) 112, 133, 303, 515
FokI GGATG 2 cut(s) 26, 448
FriOI GRGCYC 3 cut(s) 147, 244, 408
Fsp4HI GCNGC 4 cut(s) 112, 133, 303, 515
GlaI GCGC 1 cut(s) 29
GluI GCNGC 4 cut(s) 112, 133, 303, 515
GsuI CTGGAG 1 cut(s) 347
HaeIII GGCC 4 cut(s) 80, 228, 242, 380
HapII CCGG 3 cut(s) 264, 356, 367
HgaI GACGC 2 cut(s) 124, 170
HhaI GCGC 1 cut(s) 30
Hin1I GRCGYC 3 cut(s) 162, 168, 234
Hin1II CATG 2 cut(s) 125, 340
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HincII GTYRAC 2 cut(s) 363, 397
HindII GTYRAC 2 cut(s) 363, 397
HinfI GANTC 1 cut(s) 359
HpaII CCGG 3 cut(s) 264, 356, 367
HphI GGTGA 2 cut(s) 85, 280
Hpy166II GTNNAC 2 cut(s) 363, 397
Hpy188I TCNGA 5 cut(s) 70, 160, 448, 508, 533
Hpy188III TCNNGA 1 cut(s) 54
Hpy8I GTNNAC 2 cut(s) 363, 397
Hpy99I CGWCG 6 cut(s) 164, 170, 209, 236, 254, 401
HpyCH4III ACNGT 2 cut(s) 250, 418
HpyCH4IV ACGT 3 cut(s) 168, 234, 314
HpyCH4V TGCA 1 cut(s) 517
HpySE526I ACGT 3 cut(s) 168, 234, 314
Hsp92I GRCGYC 3 cut(s) 162, 168, 234
Hsp92II CATG 2 cut(s) 125, 340
HspAI GCGC 1 cut(s) 28
KspI CCGCGG 1 cut(s) 303
LmnI GCTCC 8 cut(s) 111, 150, 161, 307, 328, 373, 403, 511
Lsp1109I GCAGC 1 cut(s) 501
MaeII ACGT 3 cut(s) 168, 234, 314
MaeIII GTNAC 2 cut(s) 268, 412
MbiI CCGCTC 1 cut(s) 156
MboII GAAGA 2 cut(s) 118, 436
MhlI GDGCHC 3 cut(s) 147, 244, 408
MlsI TGGCCA 1 cut(s) 380
MluCI AATT 1 cut(s) 442
MluNI TGGCCA 1 cut(s) 380
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 2 cut(s) 183, 297
Mox20I TGGCCA 1 cut(s) 380
MscI TGGCCA 1 cut(s) 380
Msp20I TGGCCA 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 3 cut(s) 264, 356, 367
MspR9I CCNGG 5 cut(s) 77, 264, 265, 367, 382
MvaI CCWGG 2 cut(s) 77, 382
MvnI CGCG 2 cut(s) 135, 302
NciI CCSGG 3 cut(s) 264, 265, 367
NlaIII CATG 2 cut(s) 125, 340
NlaIV GGNNCC 4 cut(s) 242, 298, 347, 420
NmuCI GTSAC 2 cut(s) 268, 412
NspI RCATGY 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 140
PciI ACATGT 1 cut(s) 336
PcsI WCGNNNNNNNCGW 3 cut(s) 174, 213, 231
PkrI GCNGC 4 cut(s) 113, 134, 304, 516
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
PscI ACATGT 1 cut(s) 336
Psp124BI GAGCTC 2 cut(s) 147, 408
Psp6I CCWGG 2 cut(s) 75, 380
PspEI GGTNACC 1 cut(s) 268
PspGI CCWGG 2 cut(s) 75, 380
PspN4I GGNNCC 4 cut(s) 242, 298, 347, 420
PspOMI GGGCCC 1 cut(s) 240
PspPI GGNCC 5 cut(s) 7, 79, 240, 241, 297
PspXI VCTCGAGB 1 cut(s) 140
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
SacI GAGCTC 2 cut(s) 147, 408
SacII CCGCGG 1 cut(s) 303
SalI GTCGAC 2 cut(s) 361, 395
SatI GCNGC 4 cut(s) 112, 133, 303, 515
Sau96I GGNCC 5 cut(s) 7, 79, 240, 241, 297
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 5 cut(s) 77, 264, 265, 367, 382
SduI GDGCHC 3 cut(s) 147, 244, 408
Sfr274I CTCGAG 1 cut(s) 140
Sfr303I CCGCGG 1 cut(s) 303
SgrBI CCGCGG 1 cut(s) 303
SinI GGWCC 2 cut(s) 7, 297
SlaI CTCGAG 1 cut(s) 140
SmaI CCCGGG 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 1 cut(s) 442
SstI GAGCTC 2 cut(s) 147, 408
StyD4I CCNGG 5 cut(s) 75, 262, 263, 365, 380
TaaI ACNGT 2 cut(s) 250, 418
TaiI ACGT 3 cut(s) 171, 237, 317
TaqI TCGA 5 cut(s) 141, 177, 252, 362, 396
TaqII GACCGA 1 cut(s) 77
TasI AATT 1 cut(s) 442
TauI GCSGC 3 cut(s) 114, 135, 305
TseFI GTSAC 2 cut(s) 268, 412
TseI GCWGC 1 cut(s) 514
Tsp45I GTSAC 2 cut(s) 268, 412
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 386
TspMI CCCGGG 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 7, 297
XagI CCTNNNNNAGG 1 cut(s) 176
XapI RAATTY 1 cut(s) 442
XceI RCATGY 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 140
XmaI CCCGGG 1 cut(s) 263
XmiI GTMKAC 2 cut(s) 362, 396
ZraI GACGTC 2 cut(s) 169, 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.