Prupe.1G015400_v2.0.a1

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0007781
Physical Location & Seq
Reverse (-)
1110109 .. 1112124
2016 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G015400.1

Sequence Viewer

Length: 708 bp
ATGATTCTTTCTTTTCATAAAAACAACCACCCAATCAGGATTTTGATTCCCACCCAAGATTGGCCTTCCCTTTTCACAAGGGCATCTTTCATATTTCTATTAATCTTGTCTCAATCTCTCTCTTTCTTCTGCACCTCCACCACAGCCATTGAAGCCCAAGCCATGAAGGTCCACCCGATGCCCAAGAAGCGCAACATCACCATCCAATACAACTCAAGCAACCCACTTTCTGAAGCTGAGGCCCTTCTGGGTCTGACCCACAAGAAGCTCCGCCGCCTGCCTCATGTCTTCAGTCGGGTCCTGGAGCTCCCATTTCGCTCTGACGCCGAAGTTTTGGTCGAGGAGAATCCCGATTGCTTCCGATTTATCGCCGAAACAGATAACATCGGCGACGGCGTGAGGGCCCACACCGTCGAAATCTATCCGGGTGTGACCAAAATCGTGGTTCGGGAAAGTGGGTCGGCGGAATTGACATTGGACGAGCTTGAGCTCGACATGTGGAGGTTTCGGCTGCCCGAGTCGACTCGGCCCGAGTTGGCAAGCGCGGTTTTCGTCGACGGTGAGCTTATCGTGACGGTGCCGAAGGCCGAGGGGATCGAGAATTCGGACGGTGGAAATGGCGGTGGAGATGTTTGGGGTGATGGAGATGGGAATGGGAATGGGGGTTTCAGAGGAGGTATGGGTAACCGCCTTGTGCTTGTACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

236

Amino Acids

25.98

Weight (kDa)

5.41

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 577
AccI GTMKAC 2 cut(s) 521, 555
AccII CGCG 1 cut(s) 545
AciI CCGC 6 cut(s) 271, 274, 464, 545, 621, 688
AclWI GGATC 1 cut(s) 602
AcsI RAATTY 1 cut(s) 601
AcuI CTGAAG 2 cut(s) 252, 274
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 1 cut(s) 60
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 1 cut(s) 152
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 6 cut(s) 236, 268, 307, 484, 490, 565
AluI AGCT 6 cut(s) 236, 268, 307, 484, 490, 565
Alw21I GWGCWC 2 cut(s) 309, 492
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 1 cut(s) 602
Ama87I CYCGRG 2 cut(s) 515, 530
AoxI GGCC 5 cut(s) 62, 240, 402, 527, 585
ApaI GGGCCC 1 cut(s) 406
ApeKI GCWGC 1 cut(s) 511
ApoI RAATTY 1 cut(s) 601
ArsI GACNNNNNNTTYG 2 cut(s) 321, 353
AseI ATTAAT 1 cut(s) 101
AspLEI GCGC 2 cut(s) 192, 545
AspS9I GGNCC 6 cut(s) 169, 241, 298, 402, 403, 528
AsuC2I CCSGG 1 cut(s) 426
AsuHPI GGTGA 3 cut(s) 190, 572, 650
AvaI CYCGRG 2 cut(s) 515, 530
AvaII GGWCC 2 cut(s) 169, 298
BaeGI GKGCMC 1 cut(s) 406
BanI GGYRCC 1 cut(s) 577
BanII GRGCYC 3 cut(s) 309, 406, 492
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 2 cut(s) 309, 492
BbvCI CCTCAGC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 498
BccI CCATC 3 cut(s) 209, 635, 641
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 1 cut(s) 302
BcnI CCSGG 1 cut(s) 426
BcoDI GTCTC 1 cut(s) 114
BisI GCNGC 2 cut(s) 274, 512
BlsI GCNGC 2 cut(s) 275, 513
Bme1390I CCNGG 2 cut(s) 302, 426
Bme18I GGWCC 2 cut(s) 169, 298
BmeT110I CYCGRG 2 cut(s) 515, 530
BmgT120I GGNCC 6 cut(s) 169, 241, 298, 402, 403, 528
BmiI GGNNCC 3 cut(s) 299, 404, 579
BmrFI CCNGG 2 cut(s) 302, 426
BmsI GCATC 2 cut(s) 92, 168
BpiI GAAGAC 1 cut(s) 280
BpmI CTGGAG 1 cut(s) 323
Bpu10I CCTNAGC 1 cut(s) 237
BpuEI CTTGAG 2 cut(s) 199, 506
BpuMI CCSGG 1 cut(s) 426
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 588
Bsc4I CCNNNNNNNGG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 1 cut(s) 588
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 228
BseRI GAGGAG 2 cut(s) 356, 687
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 1 cut(s) 498
BsgI GTGCAG 1 cut(s) 115
Bsh1236I CGCG 1 cut(s) 545
BshFI GGCC 5 cut(s) 64, 242, 404, 529, 587
BshNI GGYRCC 1 cut(s) 577
BsiHKAI GWGCWC 2 cut(s) 309, 492
BsiHKCI CYCGRG 2 cut(s) 515, 530
BsiSI CCGG 1 cut(s) 425
BslI CCNNNNNNNGG 1 cut(s) 60
BsmAI GTCTC 1 cut(s) 114
BsnI GGCC 5 cut(s) 64, 242, 404, 529, 587
BsoBI CYCGRG 2 cut(s) 515, 530
Bsp120I GGGCCC 1 cut(s) 402
Bsp1286I GDGCHC 3 cut(s) 309, 406, 492
Bsp1407I TGTACA 1 cut(s) 700
Bsp143I GATC 1 cut(s) 594
BspACI CCGC 6 cut(s) 271, 274, 464, 545, 621, 688
BspANI GGCC 5 cut(s) 64, 242, 404, 529, 587
BspCNI CTCAG 1 cut(s) 229
BspFNI CGCG 1 cut(s) 545
BspLI GGNNCC 3 cut(s) 299, 404, 579
BspPI GGATC 1 cut(s) 602
BspT107I GGYRCC 1 cut(s) 577
BsrGI TGTACA 1 cut(s) 700
BssECI CCNNGG 1 cut(s) 588
BssMI GATC 1 cut(s) 594
BssNI GRCGYC 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 5 cut(s) 412, 560, 577, 611, 705
BstACI GRCGYC 1 cut(s) 324
BstAUI TGTACA 1 cut(s) 700
BstC8I GCNNGC 2 cut(s) 278, 541
BstDEI CTNAG 1 cut(s) 237
BstEII GGTNACC 1 cut(s) 683
BstF5I GGATG 1 cut(s) 201
BstFNI CGCG 1 cut(s) 545
BstHHI GCGC 2 cut(s) 192, 545
BstKTI GATC 1 cut(s) 597
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 1 cut(s) 594
BstMWI GCNNNNNNNGC 2 cut(s) 152, 187
BstNI CCWGG 1 cut(s) 302
BstNSI RCATGY 1 cut(s) 499
BstPI GGTNACC 1 cut(s) 683
BstSCI CCNGG 2 cut(s) 300, 424
BstSLI GKGCMC 1 cut(s) 406
BstUI CGCG 1 cut(s) 545
BstV1I GCAGC 1 cut(s) 498
BstV2I GAAGAC 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 442
BsuRI GGCC 5 cut(s) 64, 242, 404, 529, 587
BtsCI GGATG 1 cut(s) 201
Cac8I GCNNGC 2 cut(s) 278, 541
CfoI GCGC 2 cut(s) 192, 545
Cfr13I GGNCC 6 cut(s) 169, 241, 298, 402, 403, 528
CseI GACGC 1 cut(s) 332
Csp6I GTAC 1 cut(s) 701
CspCI CAANNNNNGTGG 2 cut(s) 130, 165
CviAII CATG 3 cut(s) 163, 284, 496
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 1 cut(s) 237
DpnI GATC 1 cut(s) 596
DpnII GATC 1 cut(s) 594
EciI GGCGGA 2 cut(s) 260, 479
Ecl136II GAGCTC 2 cut(s) 307, 490
Eco24I GRGCYC 3 cut(s) 309, 406, 492
Eco47I GGWCC 2 cut(s) 169, 298
Eco53kI GAGCTC 2 cut(s) 307, 490
Eco57I CTGAAG 2 cut(s) 252, 274
Eco88I CYCGRG 2 cut(s) 515, 530
Eco91I GGTNACC 1 cut(s) 683
EcoICRI GAGCTC 2 cut(s) 307, 490
EcoO109I RGGNCCY 3 cut(s) 241, 298, 402
EcoO65I GGTNACC 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 601
EcoRII CCWGG 1 cut(s) 300
EcoT38I GRGCYC 3 cut(s) 309, 406, 492
FaeI CATG 3 cut(s) 166, 287, 499
FaiI YATR 6 cut(s) 18, 92, 164, 285, 497, 680
FalI AAGNNNNNCTT 2 cut(s) 70, 102
FatI CATG 3 cut(s) 162, 283, 495
FblI GTMKAC 2 cut(s) 521, 555
Fnu4HI GCNGC 2 cut(s) 274, 512
FokI GGATG 1 cut(s) 188
FriOI GRGCYC 3 cut(s) 309, 406, 492
Fsp4HI GCNGC 2 cut(s) 274, 512
GlaI GCGC 2 cut(s) 191, 544
GluI GCNGC 2 cut(s) 274, 512
GsuI CTGGAG 1 cut(s) 323
HaeIII GGCC 5 cut(s) 64, 242, 404, 529, 587
HapII CCGG 1 cut(s) 425
HgaI GACGC 1 cut(s) 332
HhaI GCGC 2 cut(s) 192, 545
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 3 cut(s) 166, 287, 499
Hin6I GCGC 2 cut(s) 190, 543
HinP1I GCGC 2 cut(s) 190, 543
HincII GTYRAC 2 cut(s) 522, 556
HindII GTYRAC 2 cut(s) 522, 556
HinfI GANTC 5 cut(s) 4, 46, 346, 518, 523
HpaII CCGG 1 cut(s) 425
HphI GGTGA 3 cut(s) 190, 572, 650
Hpy166II GTNNAC 3 cut(s) 172, 522, 556
Hpy188I TCNGA 6 cut(s) 232, 255, 322, 362, 607, 671
Hpy188III TCNNGA 5 cut(s) 37, 350, 449, 571, 598
Hpy8I GTNNAC 3 cut(s) 172, 522, 556
Hpy99I CGWCG 4 cut(s) 395, 416, 557, 560
HpyAV CCTTC 4 cut(s) 75, 160, 254, 577
HpyCH4III ACNGT 5 cut(s) 412, 560, 577, 611, 705
HpyCH4V TGCA 1 cut(s) 132
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 187
HpyF3I CTNAG 1 cut(s) 237
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 3 cut(s) 166, 287, 499
HspAI GCGC 2 cut(s) 190, 543
Kzo9I GATC 1 cut(s) 594
LmnI GCTCC 3 cut(s) 273, 304, 312
LpnPI CCDG 6 cut(s) 22, 233, 287, 290, 314, 438
Lsp1109I GCAGC 1 cut(s) 498
LweI GCATC 2 cut(s) 92, 168
MaeIII GTNAC 3 cut(s) 430, 571, 683
MalI GATC 1 cut(s) 596
MboI GATC 1 cut(s) 594
MboII GAAGA 2 cut(s) 118, 280
MhlI GDGCHC 3 cut(s) 309, 406, 492
MluCI AATT 2 cut(s) 467, 601
MlyI GAGTC 2 cut(s) 517, 527
MnlI CCTC 9 cut(s) 145, 232, 291, 334, 393, 495, 583, 665, 668
MseI TTAA 1 cut(s) 101
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 2 cut(s) 302, 426
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 2 cut(s) 152, 187
NciI CCSGG 1 cut(s) 426
NdeII GATC 1 cut(s) 594
NlaIII CATG 3 cut(s) 166, 287, 499
NlaIV GGNNCC 3 cut(s) 299, 404, 579
NmeAIII GCCGAG 2 cut(s) 505, 613
NmuCI GTSAC 2 cut(s) 430, 571
NspI RCATGY 1 cut(s) 499
PciI ACATGT 1 cut(s) 495
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PfeI GAWTC 3 cut(s) 4, 46, 346
PfoI TCCNGGA 1 cut(s) 300
PkrI GCNGC 2 cut(s) 275, 513
PleI GAGTC 2 cut(s) 517, 526
PpsI GAGTC 2 cut(s) 517, 526
PpuMI RGGWCCY 1 cut(s) 298
PscI ACATGT 1 cut(s) 495
PshBI ATTAAT 1 cut(s) 101
Psp124BI GAGCTC 2 cut(s) 309, 492
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 300
PspEI GGTNACC 1 cut(s) 683
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 3 cut(s) 299, 404, 579
PspOMI GGGCCC 1 cut(s) 402
PspPI GGNCC 6 cut(s) 169, 241, 298, 402, 403, 528
PspPPI RGGWCCY 1 cut(s) 298
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
SacI GAGCTC 2 cut(s) 309, 492
SalI GTCGAC 2 cut(s) 520, 554
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 2 cut(s) 274, 512
Sau3AI GATC 1 cut(s) 594
Sau96I GGNCC 6 cut(s) 169, 241, 298, 402, 403, 528
SchI GAGTC 2 cut(s) 517, 527
ScrFI CCNGG 2 cut(s) 302, 426
SduI GDGCHC 3 cut(s) 309, 406, 492
SfaNI GCATC 2 cut(s) 92, 168
SgrDI CGTCGACG 1 cut(s) 554
SinI GGWCC 2 cut(s) 169, 298
SmlI CTYRAG 2 cut(s) 214, 485
SmoI CTYRAG 2 cut(s) 214, 485
Sse9I AATT 2 cut(s) 467, 601
SsiI CCGC 6 cut(s) 271, 274, 464, 545, 621, 688
SstI GAGCTC 2 cut(s) 309, 492
StyD4I CCNGG 2 cut(s) 300, 424
TaaI ACNGT 5 cut(s) 412, 560, 577, 611, 705
TaqI TCGA 6 cut(s) 339, 414, 492, 521, 555, 597
TasI AATT 2 cut(s) 467, 601
TatI WGTACW 1 cut(s) 700
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 3 cut(s) 4, 46, 346
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TseFI GTSAC 2 cut(s) 430, 571
TseI GCWGC 1 cut(s) 511
Tsp45I GTSAC 2 cut(s) 430, 571
TspDTI ATGAA 3 cut(s) 5, 79, 179
VpaK11BI GGWCC 2 cut(s) 169, 298
VspI ATTAAT 1 cut(s) 101
XapI RAATTY 1 cut(s) 601
XceI RCATGY 1 cut(s) 499
XmiI GTMKAC 2 cut(s) 521, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.