Rh4AG199700

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007781
Physical Location & Seq
Forward (+)
48442677 .. 48444776
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG199700.1

Sequence Viewer

Length: 693 bp
ATGATTCTTCCTTTTCATAAAAACAACCCCAACCCAATCAGGATTTTGATTCCCACCCAAGATTGGCCTTCACTTTTCACCCACAGGGCATCTTTGTTTCTCCTCCTATTATTAATCTTGGCTCTATCGTCCTCCTTCATCCCCATTGAAGCCATGAAGGTCCATCCAATCCCCAAGAGGCGCAACATCTCCATCCAGTACCATTCAAGAAGCCCACTCTCTGAAGCCCAGGCCCTCCTCGGCCTCTCCCACAAGAAGCTCCGCCGCCTCCCCCACGTCTTCAGCCGCGTCCTCGAGCTCCCCTTCCGCTCCGACGCCGACGTCCTCATCGAGGAGAACCCCGACTGCTTCCGCTTCGTCGCCGAAACCGAGAGCTTCGGCCACGACGTCAGGGCCCACACCGTCGAAATCCACCCCGGGGTGACCAAGATTGTCGTCAGGGAGAGAGGCTCCGCGGCGGAGCTGACGTTGGACGAGCTGGAGCTCGACATGTGGAGGTTCCGGCTCCCGGAGTCGACCCGGCCCGAGCTGGCCAGGGCGGTTTTTGTCGACGGGGAGCTCATTGTGACCGTGCCGAAGATGGAGGGGATGGACAATTCGGAGGGCGGAGACGGCGGCGGCGGCGGTGGTGGTGGGAGTGGGAATGGGGGGTTCAGAGGACGTATGGGAAATCGGCTGGTGCTTGTACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

230

Amino Acids

25.58

Weight (kDa)

7.15

Isoelectric Point (pI)

52.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 320
AatII GACGTC 2 cut(s) 324, 390
AccBSI CCGCTC 1 cut(s) 309
AccI GTMKAC 2 cut(s) 515, 549
AccII CGCG 2 cut(s) 288, 455
AcoI YGGCCR 2 cut(s) 379, 531
AcuI CTGAAG 2 cut(s) 243, 265
AcyI GRCGYC 3 cut(s) 315, 321, 387
AfaI GTAC 2 cut(s) 200, 687
AfiI CCNNNNNNNGG 4 cut(s) 63, 331, 418, 508
AflIII ACRYGT 1 cut(s) 489
AgsI TTSAA 2 cut(s) 149, 207
AjiI CACGTC 1 cut(s) 277
AjnI CCWGG 2 cut(s) 228, 533
AluBI AGCT 8 cut(s) 259, 298, 375, 463, 478, 484, 529, 559
AluI AGCT 8 cut(s) 259, 298, 375, 463, 478, 484, 529, 559
Alw21I GWGCWC 3 cut(s) 300, 486, 561
Alw26I GTCTC 1 cut(s) 603
Ama87I CYCGRG 3 cut(s) 293, 416, 524
AoxI GGCC 7 cut(s) 65, 231, 241, 379, 393, 521, 531
ApaI GGGCCC 1 cut(s) 397
AseI ATTAAT 1 cut(s) 113
AspLEI GCGC 1 cut(s) 183
AspS9I GGNCC 5 cut(s) 160, 232, 393, 394, 522
AsuC2I CCSGG 4 cut(s) 417, 418, 509, 520
AsuHPI GGTGA 2 cut(s) 70, 433
AvaI CYCGRG 3 cut(s) 293, 416, 524
AvaII GGWCC 1 cut(s) 160
BaeGI GKGCMC 1 cut(s) 397
BalI TGGCCA 1 cut(s) 533
BanII GRGCYC 4 cut(s) 300, 397, 486, 561
BbsI GAAGAC 1 cut(s) 271
Bbv12I GWGCWC 3 cut(s) 300, 486, 561
BccI CCATC 4 cut(s) 171, 200, 574, 583
BceAI ACGGC 1 cut(s) 628
BciT130I CCWGG 2 cut(s) 230, 535
BcnI CCSGG 4 cut(s) 417, 418, 509, 520
BcoDI GTCTC 1 cut(s) 603
BisI GCNGC 6 cut(s) 265, 286, 456, 616, 619, 622
BlsI GCNGC 6 cut(s) 266, 287, 457, 617, 620, 623
Bme1390I CCNGG 6 cut(s) 230, 417, 418, 509, 520, 535
Bme18I GGWCC 1 cut(s) 160
BmeT110I CYCGRG 3 cut(s) 293, 416, 524
BmgBI CACGTC 1 cut(s) 277
BmgT120I GGNCC 5 cut(s) 160, 232, 393, 394, 522
BmiI GGNNCC 4 cut(s) 395, 451, 500, 506
BmrFI CCNGG 6 cut(s) 230, 417, 418, 509, 520, 535
BmsI GCATC 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 271
BplI GAGNNNNNCTC 2 cut(s) 434, 466
BpmI CTGGAG 1 cut(s) 500
BpuMI CCSGG 4 cut(s) 417, 418, 509, 520
BsaHI GRCGYC 3 cut(s) 315, 321, 387
BsaJI CCNNGG 7 cut(s) 228, 238, 415, 416, 417, 453, 534
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 331, 418, 508
Bse1I ACTGG 1 cut(s) 196
BseBI CCWGG 2 cut(s) 230, 535
BseDI CCNNGG 7 cut(s) 228, 238, 415, 416, 417, 453, 534
BseGI GGATG 4 cut(s) 138, 163, 192, 594
BseLI CCNNNNNNNGG 4 cut(s) 63, 331, 418, 508
BseNI ACTGG 1 cut(s) 196
BseRI GAGGAG 3 cut(s) 92, 227, 347
BseSI GKGCMC 1 cut(s) 397
Bsh1236I CGCG 2 cut(s) 288, 455
BshFI GGCC 7 cut(s) 67, 233, 243, 381, 395, 523, 533
BsiHKAI GWGCWC 3 cut(s) 300, 486, 561
BsiHKCI CYCGRG 3 cut(s) 293, 416, 524
BsiSI CCGG 4 cut(s) 417, 502, 509, 520
BslI CCNNNNNNNGG 4 cut(s) 63, 331, 418, 508
BsmAI GTCTC 1 cut(s) 603
BsmBI CGTCTC 1 cut(s) 603
BsnI GGCC 7 cut(s) 67, 233, 243, 381, 395, 523, 533
BsoBI CYCGRG 3 cut(s) 293, 416, 524
Bsp120I GGGCCC 1 cut(s) 393
Bsp1286I GDGCHC 4 cut(s) 300, 397, 486, 561
Bsp1407I TGTACA 1 cut(s) 685
BspANI GGCC 7 cut(s) 67, 233, 243, 381, 395, 523, 533
BspFNI CGCG 2 cut(s) 288, 455
BspLI GGNNCC 4 cut(s) 395, 451, 500, 506
BsrBI CCGCTC 1 cut(s) 309
BsrGI TGTACA 1 cut(s) 685
BsrI ACTGG 1 cut(s) 196
BssECI CCNNGG 7 cut(s) 228, 238, 415, 416, 417, 453, 534
BssNI GRCGYC 3 cut(s) 315, 321, 387
Bst2UI CCWGG 2 cut(s) 230, 535
Bst4CI ACNGT 3 cut(s) 403, 571, 690
BstACI GRCGYC 3 cut(s) 315, 321, 387
BstAUI TGTACA 1 cut(s) 685
BstC8I GCNNGC 1 cut(s) 531
BstDSI CCRYGG 1 cut(s) 453
BstEII GGTNACC 1 cut(s) 421
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 4 cut(s) 138, 163, 192, 594
BstFNI CGCG 2 cut(s) 288, 455
BstHHI GCGC 1 cut(s) 183
BstMAI GTCTC 1 cut(s) 603
BstMWI GCNNNNNNNGC 2 cut(s) 612, 621
BstNI CCWGG 2 cut(s) 230, 535
BstNSI RCATGY 1 cut(s) 493
BstPI GGTNACC 1 cut(s) 421
BstSCI CCNGG 6 cut(s) 228, 415, 416, 507, 518, 533
BstSLI GKGCMC 1 cut(s) 397
BstUI CGCG 2 cut(s) 288, 455
BstV2I GAAGAC 1 cut(s) 271
BsuRI GGCC 7 cut(s) 67, 233, 243, 381, 395, 523, 533
BtgI CCRYGG 1 cut(s) 453
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 4 cut(s) 138, 163, 192, 594
Cac8I GCNNGC 1 cut(s) 531
CfoI GCGC 1 cut(s) 183
Cfr13I GGNCC 5 cut(s) 160, 232, 393, 394, 522
Cfr42I CCGCGG 1 cut(s) 456
Cfr9I CCCGGG 1 cut(s) 416
CseI GACGC 2 cut(s) 277, 323
Csp6I GTAC 2 cut(s) 199, 686
CviAII CATG 2 cut(s) 154, 490
CviQI GTAC 2 cut(s) 199, 686
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 2 cut(s) 379, 531
EciI GGCGGA 3 cut(s) 251, 473, 621
Ecl136II GAGCTC 3 cut(s) 298, 484, 559
Eco24I GRGCYC 4 cut(s) 300, 397, 486, 561
Eco47I GGWCC 1 cut(s) 160
Eco53kI GAGCTC 3 cut(s) 298, 484, 559
Eco57I CTGAAG 2 cut(s) 243, 265
Eco88I CYCGRG 3 cut(s) 293, 416, 524
Eco91I GGTNACC 1 cut(s) 421
EcoICRI GAGCTC 3 cut(s) 298, 484, 559
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 2 cut(s) 232, 393
EcoO65I GGTNACC 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 228, 533
EcoT38I GRGCYC 4 cut(s) 300, 397, 486, 561
Esp3I CGTCTC 1 cut(s) 603
FaeI CATG 2 cut(s) 157, 493
FaiI YATR 4 cut(s) 18, 155, 491, 665
FatI CATG 2 cut(s) 153, 489
FblI GTMKAC 2 cut(s) 515, 549
Fnu4HI GCNGC 6 cut(s) 265, 286, 456, 616, 619, 622
FokI GGATG 4 cut(s) 125, 150, 179, 601
FriOI GRGCYC 4 cut(s) 300, 397, 486, 561
Fsp4HI GCNGC 6 cut(s) 265, 286, 456, 616, 619, 622
GlaI GCGC 1 cut(s) 182
GluI GCNGC 6 cut(s) 265, 286, 456, 616, 619, 622
GsuI CTGGAG 1 cut(s) 500
HaeIII GGCC 7 cut(s) 67, 233, 243, 381, 395, 523, 533
HapII CCGG 4 cut(s) 417, 502, 509, 520
HgaI GACGC 2 cut(s) 277, 323
HhaI GCGC 1 cut(s) 183
Hin1I GRCGYC 3 cut(s) 315, 321, 387
Hin1II CATG 2 cut(s) 157, 493
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HincII GTYRAC 2 cut(s) 516, 550
HindII GTYRAC 2 cut(s) 516, 550
HinfI GANTC 3 cut(s) 4, 49, 512
HpaII CCGG 4 cut(s) 417, 502, 509, 520
HphI GGTGA 2 cut(s) 70, 433
Hpy166II GTNNAC 2 cut(s) 516, 550
Hpy188I TCNGA 4 cut(s) 223, 313, 601, 656
Hpy188III TCNNGA 2 cut(s) 40, 207
Hpy8I GTNNAC 2 cut(s) 516, 550
Hpy99I CGWCG 6 cut(s) 317, 323, 362, 389, 407, 554
HpyAV CCTTC 4 cut(s) 78, 145, 151, 313
HpyCH4III ACNGT 3 cut(s) 403, 571, 690
HpyCH4IV ACGT 5 cut(s) 276, 321, 387, 467, 661
HpyF10VI GCNNNNNNNGC 2 cut(s) 612, 621
HpySE526I ACGT 5 cut(s) 276, 321, 387, 467, 661
Hsp92I GRCGYC 3 cut(s) 315, 321, 387
Hsp92II CATG 2 cut(s) 157, 493
HspAI GCGC 1 cut(s) 181
KspI CCGCGG 1 cut(s) 456
LmnI GCTCC 8 cut(s) 264, 303, 314, 455, 460, 481, 510, 556
LweI GCATC 1 cut(s) 98
MaeII ACGT 5 cut(s) 276, 321, 387, 467, 661
MaeIII GTNAC 2 cut(s) 421, 565
MbiI CCGCTC 1 cut(s) 309
MboII GAAGA 2 cut(s) 271, 589
MhlI GDGCHC 4 cut(s) 300, 397, 486, 561
MlsI TGGCCA 1 cut(s) 533
MluCI AATT 1 cut(s) 595
MluNI TGGCCA 1 cut(s) 533
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 2 cut(s) 336, 450
Mox20I TGGCCA 1 cut(s) 533
MscI TGGCCA 1 cut(s) 533
MseI TTAA 1 cut(s) 113
Msp20I TGGCCA 1 cut(s) 533
MspA1I CMGCKG 1 cut(s) 455
MspI CCGG 4 cut(s) 417, 502, 509, 520
MspR9I CCNGG 6 cut(s) 230, 417, 418, 509, 520, 535
MvaI CCWGG 2 cut(s) 230, 535
MvnI CGCG 2 cut(s) 288, 455
MwoI GCNNNNNNNGC 2 cut(s) 612, 621
NciI CCSGG 4 cut(s) 417, 418, 509, 520
NlaIII CATG 2 cut(s) 157, 493
NlaIV GGNNCC 4 cut(s) 395, 451, 500, 506
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 2 cut(s) 421, 565
NspI RCATGY 1 cut(s) 493
PaeR7I CTCGAG 1 cut(s) 293
PciI ACATGT 1 cut(s) 489
PcsI WCGNNNNNNNCGW 3 cut(s) 327, 366, 384
PfeI GAWTC 2 cut(s) 4, 49
PfoI TCCNGGA 1 cut(s) 507
PkrI GCNGC 6 cut(s) 266, 287, 457, 617, 620, 623
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
PscI ACATGT 1 cut(s) 489
PshBI ATTAAT 1 cut(s) 113
Psp124BI GAGCTC 3 cut(s) 300, 486, 561
Psp6I CCWGG 2 cut(s) 228, 533
PspEI GGTNACC 1 cut(s) 421
PspGI CCWGG 2 cut(s) 228, 533
PspN4I GGNNCC 4 cut(s) 395, 451, 500, 506
PspOMI GGGCCC 1 cut(s) 393
PspPI GGNCC 5 cut(s) 160, 232, 393, 394, 522
PspXI VCTCGAGB 1 cut(s) 293
RsaI GTAC 2 cut(s) 200, 687
RsaNI GTAC 2 cut(s) 199, 686
SacI GAGCTC 3 cut(s) 300, 486, 561
SacII CCGCGG 1 cut(s) 456
SalI GTCGAC 2 cut(s) 514, 548
SaqAI TTAA 1 cut(s) 113
SatI GCNGC 6 cut(s) 265, 286, 456, 616, 619, 622
Sau96I GGNCC 5 cut(s) 160, 232, 393, 394, 522
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 6 cut(s) 230, 417, 418, 509, 520, 535
SduI GDGCHC 4 cut(s) 300, 397, 486, 561
SfaNI GCATC 1 cut(s) 98
Sfr274I CTCGAG 1 cut(s) 293
Sfr303I CCGCGG 1 cut(s) 456
SgrBI CCGCGG 1 cut(s) 456
SinI GGWCC 1 cut(s) 160
SlaI CTCGAG 1 cut(s) 293
SmaI CCCGGG 1 cut(s) 418
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 1 cut(s) 595
SstI GAGCTC 3 cut(s) 300, 486, 561
StyD4I CCNGG 6 cut(s) 228, 415, 416, 507, 518, 533
TaaI ACNGT 3 cut(s) 403, 571, 690
TaiI ACGT 5 cut(s) 279, 324, 390, 470, 664
TaqI TCGA 6 cut(s) 294, 330, 405, 486, 515, 549
TasI AATT 1 cut(s) 595
TatI WGTACW 1 cut(s) 685
TauI GCSGC 6 cut(s) 267, 288, 458, 618, 621, 624
TfiI GAWTC 2 cut(s) 4, 49
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TseFI GTSAC 2 cut(s) 421, 565
Tsp45I GTSAC 2 cut(s) 421, 565
TspDTI ATGAA 3 cut(s) 5, 127, 170
TspMI CCCGGG 1 cut(s) 416
VpaK11BI GGWCC 1 cut(s) 160
VspI ATTAAT 1 cut(s) 113
XagI CCTNNNNNAGG 1 cut(s) 329
XceI RCATGY 1 cut(s) 493
XhoI CTCGAG 1 cut(s) 293
XmaI CCCGGG 1 cut(s) 416
XmiI GTMKAC 2 cut(s) 515, 549
ZraI GACGTC 2 cut(s) 322, 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.