Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0007781

Physical Location & Seq

Genomic Coordinates Reverse (-)

8165173 .. 8167382

Gene Structure 2210 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g06220.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1704 bp

ATGAAGGTCCACCCCAAGCCCAGGAAGCGCAACATCACCGTCCAATACAGATCGAGAAACCCACTTTCTGAAGCGCAGGCACTTTTGGGTCTGACCCATAAGAAGCTCTGCCGTCTCCCCCATGTCTTCAGCCGGATCCTGGAGCTCCCCTTTCGGTCCGACGCTGAAGTTCTGGTCCAGGAGAATCCCGATTGCTTCCGATTCGTCGCCGAAGCCGATAATATCGGCGACGGCGTAAGGACCCACACCATCGAAATCCATCCCGGGGTGACCAAAATCGTGGTTCGCGAAAGTGGATCGGCGGAATTGACGTTGGACGAGCTCGAGCTCGACATGTGGAGGTTTCGGCTGCCGGAATCGACTCGACCCGAGTTGGCCAGCGCGGTTTTCGTGGACGGGGAGCTTATCGTGACGGTGCCGAAGGATGTGGGAGTGGAGAATGCGGACGGAGAGAGAGAATTGCGTCGCCATCGCCACTACCGCCGCCGCATCGTCGGGTTTAGCTTCGGAGAGTCAGCAGAGAGAACTCAAGGAGACCATCATCTCCAATCTTCCATTGGAGAAGAGCTCCAGACCTCCGACCGCAGCACCTACGAGGCCACTACTTCTCCGGAGACGGTGGCGGCGACTCTCCCTCCTTCACTAGCTCCGAGTCCAGCTTCAAAGTCAACTCATCCAACAAAAACCACCACTAGCGACAAATCCACCATCAGACCCAGCAACGGCAGGACAAGAGTCCCAGCCGGACTCTCAGTCCAATCCGCGGCGCCGGCGATGTGCTGCTGCAGTGGGGCCAGAGAAAGAGGTCGAGAGTCTCCAGGACCGAGATCCGAGCTGTCACCGATGAGTCCTCTTCGTCGGCTCAAGCGAGACAAGCTTCGAAGCTGCAGAGGAGAGACAAGTCCTTGGTCCGATCGCCTCCGCCAGCACGGCGTCCACTGCGTCCAAGGGGATCTAACCCAGAAAAAGGATGTGGAAAAAGCTCTGCGGGGAGCAGACTGTGTGTTCCACCTTGCTTCGTATGGCATGTCAGGAAAAGAAACGCTGCAGTTCGGCCATATCTTGGACGCGTGTCTCGAGTTCGGGATTCAAAGGCTGGTTTATGTGAGCACATATAATGTTGTGTTTGGGGGACAGGAGATTGTCAATGGGAACGAGGCCTTGCGCTATTTCCCTATCGAGAACGATGTCGATCCATATGGCCGGAGTAAATCAATTGCCGAGCAGTTGGTCTTAAAGTACAATGCTCGTCCTTTCAGGAAGAAGACTGGGAAGTGTCTTTATACCTGTGCAGTTCGTCCAGCTGCTATCTATGGACCAGGTGAAGAAAGGCACTTTCCAAGGATAATGTCTCTGGCAAAGTTAGGCCTGATTTCGTTCAAAATTGGCGAACCAAATGTGAAAACAGACTGGATTTATGTGGATTTACCTATTGGAGCAAATGAATTAAACAGAGGACAGACTGCACGGAAGGATGAGTTAAGTCTGTCTGATCTCTTAAATCTTGAAATAAAATGGACCTCTAGGCCAGAGAAAATTGAGACTGATCTCTCAAATGAGAACCCCAATCTACCCAAATTACCAGATTTGGCTGGAGTTAATCTCGACAACTTTTTCTTTGAACGAAACAAAGACCGTGCTTTGGGTAGTTCTGAAGGGCTGTTCGGGTCTAACACACAGATTGTCAGTAAAGAGAGGTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

3Beta_HSD DUF4499

Protein Analysis

568

Amino Acids

63.44

Weight (kDa)

8.82

Isoelectric Point (pI)

49.38

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007781)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22530 AT4G14830
fragaria_vesca	FvH4_4g16300 FvH4_4g16300
malus_domestica	MD13G1194500.v1.1 MD16G1195400.v1.1
prunus_persica	Prupe.1G015400_v2.0.a1
pyrus_communis	pycom05g06220 pycom13g16690 pycom16g16460
rosa_chinensis	RchiOBHm_Chr4g0415991
rosa_laevigata	RLG00000008025
rosa_multiflora	Rmu_sc0005415.1_g000006
rosa_roxburghii	Rroxscaffold_5G00359840
rosa_rugosa	Rorug02G0491700 Rorug03G0025900 Rorug04G0137200 Rorug06G0075100
rosa_samantha	Rh4AG199700 Rh4BG197200 Rh4CG210700 Rh5BG016200 Rh6BG368700
rosa_wichuraiana	Rw4G016930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	415, 766
AccB7I	CCANNNNNTGG	1 cut(s)	1063
AccII	CGCG	4 cut(s)	288, 383, 764, 1070
AccIII	TCCGGA	1 cut(s)	610
AclWI	GGATC	6 cut(s)	130, 143, 304, 822, 960, 1187
AcoI	YGGCCR	3 cut(s)	375, 1054, 1201
AcuI	CTGAAG	4 cut(s)	90, 112, 186, 1674
AcyI	GRCGYC	2 cut(s)	767, 933
AfaI	GTAC	1 cut(s)	1241
AfiI	CCNNNNNNNGG	8 cut(s)	21, 139, 265, 722, 763, 967, 1063, 1642
AflIII	ACRYGT	2 cut(s)	333, 1068
AgsI	TTSAA	5 cut(s)	663, 1091, 1381, 1508, 1622
AhdI	GACNNNNNGTC	1 cut(s)	752
AjnI	CCWGG	5 cut(s)	20, 138, 177, 817, 1318
Alw21I	GWGCWC	5 cut(s)	147, 324, 330, 570, 1112
Alw26I	GTCTC	9 cut(s)	119, 528, 608, 819, 864, 890, 1079, 1356, 1535
AlwI	GGATC	6 cut(s)	130, 143, 304, 822, 960, 1187
Ama87I	CYCGRG	4 cut(s)	263, 323, 368, 1076
Aor13HI	TCCGGA	1 cut(s)	610
AoxI	GGCC	8 cut(s)	375, 597, 792, 1054, 1158, 1201, 1366, 1526
ApeKI	GCWGC	7 cut(s)	349, 585, 780, 783, 885, 1045, 1304
AspLEI	GCGC	5 cut(s)	30, 76, 383, 769, 1167
AspS9I	GGNCC	9 cut(s)	7, 156, 175, 240, 792, 821, 909, 1316, 1518
AsuC2I	CCSGG	2 cut(s)	264, 265
AsuHPI	GGTGA	4 cut(s)	28, 280, 831, 1334
AsuII	TTCGAA	1 cut(s)	880
AvaI	CYCGRG	4 cut(s)	263, 323, 368, 1076
AvaII	GGWCC	8 cut(s)	7, 156, 175, 240, 821, 909, 1316, 1518
BalI	TGGCCA	1 cut(s)	377
BamHI	GGATCC	1 cut(s)	135
BanI	GGYRCC	2 cut(s)	415, 766
BanII	GRGCYC	4 cut(s)	147, 324, 330, 570
BbsI	GAAGAC	2 cut(s)	118, 1271
Bbv12I	GWGCWC	5 cut(s)	147, 324, 330, 570, 1112
BbvI	GCAGC	7 cut(s)	336, 597, 767, 770, 872, 1032, 1291
BccI	CCATC	5 cut(s)	257, 267, 477, 546, 716
BceAI	ACGGC	4 cut(s)	96, 247, 739, 946
BciT130I	CCWGG	5 cut(s)	22, 140, 179, 819, 1320
BcnI	CCSGG	2 cut(s)	264, 265
BcoDI	GTCTC	9 cut(s)	119, 528, 608, 819, 864, 890, 1079, 1356, 1535
BfaI	CTAG	3 cut(s)	644, 693, 1524
BfmI	CTRYAG	3 cut(s)	784, 886, 1046
BfoI	RGCGCY	1 cut(s)	770
BglI	GCCNNNNNGGC	1 cut(s)	930
Bme1390I	CCNGG	7 cut(s)	22, 140, 179, 264, 265, 819, 1320
Bme18I	GGWCC	8 cut(s)	7, 156, 175, 240, 821, 909, 1316, 1518
BmeRI	GACNNNNNGTC	1 cut(s)	752
BmeT110I	CYCGRG	4 cut(s)	263, 323, 368, 1076
BmgT120I	GGNCC	9 cut(s)	7, 156, 175, 240, 792, 821, 909, 1316, 1518
BmiI	GGNNCC	5 cut(s)	137, 242, 417, 768, 793
BmrFI	CCNGG	7 cut(s)	22, 140, 179, 264, 265, 819, 1320
BmrI	ACTGGG	1 cut(s)	1278
BmsI	GCATC	1 cut(s)	498
BmuI	ACTGGG	1 cut(s)	1278
BoxI	GACNNNNGTC	2 cut(s)	734, 1071
BpiI	GAAGAC	2 cut(s)	118, 1271
BplI	GAGNNNNNCTC	4 cut(s)	552, 584, 1587, 1619
BpmI	CTGGAG	4 cut(s)	161, 554, 801, 1614
Bpu14I	TTCGAA	1 cut(s)	880
BpuEI	CTTGAG	2 cut(s)	513, 848
BpuMI	CCSGG	2 cut(s)	264, 265
BsaBI	GATNNNNATC	1 cut(s)	1191
BsaHI	GRCGYC	2 cut(s)	767, 933
BsaI	GGTCTC	1 cut(s)	528
BsaJI	CCNNGG	7 cut(s)	20, 263, 264, 762, 905, 946, 1340
BsaWI	WCCGGW	1 cut(s)	610
BsaXI	ACNNNNNCTCC	4 cut(s)	592, 619, 622, 649
Bsc4I	CCNNNNNNNGG	8 cut(s)	21, 139, 265, 722, 763, 967, 1063, 1642
Bse118I	RCCGGY	1 cut(s)	769
Bse1I	ACTGG	2 cut(s)	1273, 1415
Bse8I	GATNNNNATC	1 cut(s)	1191
BseAI	TCCGGA	1 cut(s)	610
BseBI	CCWGG	5 cut(s)	22, 140, 179, 819, 1320
BseDI	CCNNGG	7 cut(s)	20, 263, 264, 762, 905, 946, 1340
BseGI	GGATG	5 cut(s)	259, 430, 673, 976, 1480
BseJI	GATNNNNATC	1 cut(s)	1191
BseLI	CCNNNNNNNGG	8 cut(s)	21, 139, 265, 722, 763, 967, 1063, 1642
BseMII	CTCAG	1 cut(s)	765
BseNI	ACTGG	2 cut(s)	1273, 1415
BseRI	GAGGAG	1 cut(s)	906
BseXI	GCAGC	7 cut(s)	336, 597, 767, 770, 872, 1032, 1291
BseYI	CCCAGC	2 cut(s)	716, 739
BsgI	GTGCAG	2 cut(s)	1311, 1449
Bsh1236I	CGCG	4 cut(s)	288, 383, 764, 1070
Bsh1285I	CGRYCG	2 cut(s)	583, 916
BshFI	GGCC	8 cut(s)	377, 599, 794, 1056, 1160, 1203, 1368, 1528
BshNI	GGYRCC	2 cut(s)	415, 766
BsiEI	CGRYCG	2 cut(s)	583, 916
BsiHKAI	GWGCWC	5 cut(s)	147, 324, 330, 570, 1112
BsiHKCI	CYCGRG	4 cut(s)	263, 323, 368, 1076
BsiSI	CCGG	7 cut(s)	133, 264, 353, 611, 744, 770, 1204
BslFI	GGGAC	2 cut(s)	722, 1146
BslI	CCNNNNNNNGG	8 cut(s)	21, 139, 265, 722, 763, 967, 1063, 1642
BsmAI	GTCTC	9 cut(s)	119, 528, 608, 819, 864, 890, 1079, 1356, 1535
BsmBI	CGTCTC	2 cut(s)	119, 608
BsmFI	GGGAC	2 cut(s)	722, 1146
BsmI	GAATGC	1 cut(s)	445
BsnI	GGCC	8 cut(s)	377, 599, 794, 1056, 1160, 1203, 1368, 1528
Bso31I	GGTCTC	1 cut(s)	528
BsoBI	CYCGRG	4 cut(s)	263, 323, 368, 1076
Bsp119I	TTCGAA	1 cut(s)	880
Bsp1286I	GDGCHC	5 cut(s)	147, 324, 330, 570, 1112
Bsp13I	TCCGGA	1 cut(s)	610
Bsp143I	GATC	9 cut(s)	50, 135, 296, 827, 913, 952, 1192, 1492, 1546
Bsp68I	TCGCGA	1 cut(s)	288
BspANI	GGCC	8 cut(s)	377, 599, 794, 1056, 1160, 1203, 1368, 1528
BspCNI	CTCAG	1 cut(s)	764
BspEI	TCCGGA	1 cut(s)	610
BspFNI	CGCG	4 cut(s)	288, 383, 764, 1070
BspLI	GGNNCC	5 cut(s)	137, 242, 417, 768, 793
BspMAI	CTGCAG	3 cut(s)	788, 890, 1050
BspPI	GGATC	6 cut(s)	130, 143, 304, 822, 960, 1187
BspQI	GCTCTTC	1 cut(s)	558
BspT104I	TTCGAA	1 cut(s)	880
BspT107I	GGYRCC	2 cut(s)	415, 766
BspTNI	GGTCTC	1 cut(s)	528
BsrFI	RCCGGY	1 cut(s)	769
BsrI	ACTGG	2 cut(s)	1273, 1415
BssAI	RCCGGY	1 cut(s)	769
BssECI	CCNNGG	7 cut(s)	20, 263, 264, 762, 905, 946, 1340
BssMI	GATC	9 cut(s)	50, 135, 296, 827, 913, 952, 1192, 1492, 1546
BssNI	GRCGYC	2 cut(s)	767, 933
BssT1I	CCWWGG	3 cut(s)	905, 946, 1340
Bst2UI	CCWGG	5 cut(s)	22, 140, 179, 819, 1320
Bst4CI	ACNGT	5 cut(s)	40, 415, 619, 1001, 1637
Bst6I	CTCTTC	2 cut(s)	558, 858
BstACI	GRCGYC	2 cut(s)	767, 933
BstBI	TTCGAA	1 cut(s)	880
BstC8I	GCNNGC	4 cut(s)	78, 379, 771, 926
BstDEI	CTNAG	1 cut(s)	751
BstDSI	CCRYGG	1 cut(s)	762
BstEII	GGTNACC	1 cut(s)	268
BstF5I	GGATG	5 cut(s)	259, 430, 673, 976, 1480
BstFNI	CGCG	4 cut(s)	288, 383, 764, 1070
BstH2I	RGCGCY	1 cut(s)	770
BstHHI	GCGC	5 cut(s)	30, 76, 383, 769, 1167
BstKTI	GATC	9 cut(s)	53, 138, 299, 830, 916, 955, 1195, 1495, 1549
BstMAI	GTCTC	9 cut(s)	119, 528, 608, 819, 864, 890, 1079, 1356, 1535
BstMBI	GATC	9 cut(s)	50, 135, 296, 827, 913, 952, 1192, 1492, 1546
BstMCI	CGRYCG	2 cut(s)	583, 916
BstMWI	GCNNNNNNNGC	6 cut(s)	25, 480, 770, 874, 930, 939
BstNI	CCWGG	5 cut(s)	22, 140, 179, 819, 1320
BstNSI	RCATGY	2 cut(s)	337, 1030
BstPAI	GACNNNNGTC	2 cut(s)	734, 1071
BstPI	GGTNACC	1 cut(s)	268
BstSCI	CCNGG	7 cut(s)	20, 138, 177, 262, 263, 817, 1318
BstSFI	CTRYAG	3 cut(s)	784, 886, 1046
BstUI	CGCG	4 cut(s)	288, 383, 764, 1070
BstV1I	GCAGC	7 cut(s)	336, 597, 767, 770, 872, 1032, 1291
BstV2I	GAAGAC	2 cut(s)	118, 1271
BstX2I	RGATCY	3 cut(s)	135, 827, 952
BstXI	CCANNNNNNTGG	1 cut(s)	280
BstYI	RGATCY	3 cut(s)	135, 827, 952
BsuRI	GGCC	8 cut(s)	377, 599, 794, 1056, 1160, 1203, 1368, 1528
BtgI	CCRYGG	1 cut(s)	762
BtgZI	GCGATG	2 cut(s)	455, 788
BtsCI	GGATG	5 cut(s)	259, 430, 673, 976, 1480
BtsI	GCAGTG	2 cut(s)	793, 937
BtsIMutI	CAGTG	2 cut(s)	793, 937
BtuMI	TCGCGA	1 cut(s)	288
Cac8I	GCNNGC	4 cut(s)	78, 379, 771, 926
CfoI	GCGC	5 cut(s)	30, 76, 383, 769, 1167
Cfr10I	RCCGGY	1 cut(s)	769
Cfr13I	GGNCC	9 cut(s)	7, 156, 175, 240, 792, 821, 909, 1316, 1518
Cfr42I	CCGCGG	1 cut(s)	765
Cfr9I	CCCGGG	1 cut(s)	263
CpoI	CGGWCCG	1 cut(s)	156
CseI	GACGC	5 cut(s)	170, 452, 922, 931, 1076
CsiI	ACCWGGT	1 cut(s)	1318
Csp6I	GTAC	1 cut(s)	1240
CspI	CGGWCCG	1 cut(s)	156
CviAII	CATG	3 cut(s)	122, 334, 1027
CviQI	GTAC	1 cut(s)	1240
DdeI	CTNAG	1 cut(s)	751
DinI	GGCGCC	1 cut(s)	768
DpnI	GATC	9 cut(s)	52, 137, 298, 829, 915, 954, 1194, 1494, 1548
DpnII	GATC	9 cut(s)	50, 135, 296, 827, 913, 952, 1192, 1492, 1546
DriI	GACNNNNNGTC	1 cut(s)	752
EaeI	YGGCCR	3 cut(s)	375, 1054, 1201
Eam1104I	CTCTTC	2 cut(s)	558, 858
Eam1105I	GACNNNNNGTC	1 cut(s)	752
EarI	CTCTTC	2 cut(s)	558, 858
EciI	GGCGGA	2 cut(s)	317, 911
Ecl136II	GAGCTC	4 cut(s)	145, 322, 328, 568
Eco130I	CCWWGG	3 cut(s)	905, 946, 1340
Eco147I	AGGCCT	2 cut(s)	1160, 1368
Eco24I	GRGCYC	4 cut(s)	147, 324, 330, 570
Eco31I	GGTCTC	1 cut(s)	528
Eco47I	GGWCC	8 cut(s)	7, 156, 175, 240, 821, 909, 1316, 1518
Eco53kI	GAGCTC	4 cut(s)	145, 322, 328, 568
Eco57I	CTGAAG	4 cut(s)	90, 112, 186, 1674
Eco88I	CYCGRG	4 cut(s)	263, 323, 368, 1076
Eco91I	GGTNACC	1 cut(s)	268
EcoICRI	GAGCTC	4 cut(s)	145, 322, 328, 568
EcoO109I	RGGNCCY	1 cut(s)	240
EcoO65I	GGTNACC	1 cut(s)	268
EcoRII	CCWGG	5 cut(s)	20, 138, 177, 817, 1318
EcoT14I	CCWWGG	3 cut(s)	905, 946, 1340
EcoT38I	GRGCYC	4 cut(s)	147, 324, 330, 570
EgeI	GGCGCC	1 cut(s)	768
EheI	GGCGCC	1 cut(s)	768
ErhI	CCWWGG	3 cut(s)	905, 946, 1340
Esp3I	CGTCTC	2 cut(s)	119, 608
FaeI	CATG	3 cut(s)	125, 337, 1030
FaqI	GGGAC	2 cut(s)	722, 1146
FatI	CATG	3 cut(s)	121, 333, 1026
FauI	CCCGC	1 cut(s)	981
FauNDI	CATATG	1 cut(s)	1198
FokI	GGATG	5 cut(s)	246, 437, 660, 983, 1487
FriOI	GRGCYC	4 cut(s)	147, 324, 330, 570
FspBI	CTAG	3 cut(s)	644, 693, 1524
GlaI	GCGC	5 cut(s)	29, 75, 382, 768, 1166
GsaI	CCCAGC	2 cut(s)	720, 743
GsuI	CTGGAG	4 cut(s)	161, 554, 801, 1614
HaeII	RGCGCY	1 cut(s)	770
HaeIII	GGCC	8 cut(s)	377, 599, 794, 1056, 1160, 1203, 1368, 1528
HapII	CCGG	7 cut(s)	133, 264, 353, 611, 744, 770, 1204
HgaI	GACGC	5 cut(s)	170, 452, 922, 931, 1076
HhaI	GCGC	5 cut(s)	30, 76, 383, 769, 1167
Hin1I	GRCGYC	2 cut(s)	767, 933
Hin1II	CATG	3 cut(s)	125, 337, 1030
Hin6I	GCGC	5 cut(s)	28, 74, 381, 767, 1165
HinP1I	GCGC	5 cut(s)	28, 74, 381, 767, 1165
HincII	GTYRAC	1 cut(s)	669
HindII	GTYRAC	1 cut(s)	669
HindIII	AAGCTT	1 cut(s)	875
HpaII	CCGG	7 cut(s)	133, 264, 353, 611, 744, 770, 1204
HphI	GGTGA	4 cut(s)	28, 280, 831, 1334
Hpy166II	GTNNAC	4 cut(s)	10, 394, 669, 937
Hpy8I	GTNNAC	4 cut(s)	10, 394, 669, 937
Hpy99I	CGWCG	6 cut(s)	164, 209, 233, 468, 497, 861
HpyAV	CCTTC	4 cut(s)	415, 648, 1465, 1649
HpyCH4III	ACNGT	5 cut(s)	40, 415, 619, 1001, 1637
HpyCH4IV	ACGT	1 cut(s)	311
HpyCH4V	TGCA	5 cut(s)	786, 888, 1048, 1292, 1466
HpyF10VI	GCNNNNNNNGC	6 cut(s)	25, 480, 770, 874, 930, 939
HpyF3I	CTNAG	1 cut(s)	751
HpySE526I	ACGT	1 cut(s)	311
Hsp92I	GRCGYC	2 cut(s)	767, 933
Hsp92II	CATG	3 cut(s)	125, 337, 1030
HspAI	GCGC	5 cut(s)	28, 74, 381, 767, 1165
KasI	GGCGCC	1 cut(s)	766
Kpn2I	TCCGGA	1 cut(s)	610
KroI	GCCGGC	1 cut(s)	769
KroNI	GCCGGC	1 cut(s)	771
KspI	CCGCGG	1 cut(s)	765
Kzo9I	GATC	9 cut(s)	50, 135, 296, 827, 913, 952, 1192, 1492, 1546
LguI	GCTCTTC	1 cut(s)	558
LmnI	GCTCC	7 cut(s)	142, 150, 400, 573, 652, 992, 1436
Lsp1109I	GCAGC	7 cut(s)	336, 597, 767, 770, 872, 1032, 1291
LweI	GCATC	1 cut(s)	498
MabI	ACCWGGT	1 cut(s)	1318
MaeI	CTAG	3 cut(s)	644, 693, 1524
MaeII	ACGT	1 cut(s)	311
MaeIII	GTNAC	3 cut(s)	268, 409, 837
MalI	GATC	9 cut(s)	52, 137, 298, 829, 915, 954, 1194, 1494, 1548
MboI	GATC	9 cut(s)	50, 135, 296, 827, 913, 952, 1192, 1492, 1546
MboII	GAAGA	7 cut(s)	118, 543, 575, 845, 1273, 1276, 1337
MfeI	CAATTG	1 cut(s)	1215
MflI	RGATCY	3 cut(s)	135, 827, 952
MhlI	GDGCHC	5 cut(s)	147, 324, 330, 570, 1112
MlsI	TGGCCA	1 cut(s)	377
MluCI	AATT	7 cut(s)	305, 458, 1215, 1383, 1445, 1536, 1577
MluI	ACGCGT	1 cut(s)	1068
MluNI	TGGCCA	1 cut(s)	377
Mly113I	GGCGCC	1 cut(s)	767
MlyI	GAGTC	8 cut(s)	355, 521, 622, 661, 741, 744, 821, 856
MmeI	TCCRAC	4 cut(s)	183, 294, 603, 701
Mox20I	TGGCCA	1 cut(s)	377
MreI	CGCCGGCG	1 cut(s)	769
MroI	TCCGGA	1 cut(s)	610
MroNI	GCCGGC	1 cut(s)	769
MscI	TGGCCA	1 cut(s)	377
MseI	TTAA	5 cut(s)	1235, 1448, 1481, 1499, 1599
Msp20I	TGGCCA	1 cut(s)	377
MspA1I	CMGCKG	2 cut(s)	764, 1304
MspI	CCGG	7 cut(s)	133, 264, 353, 611, 744, 770, 1204
MspR9I	CCNGG	7 cut(s)	22, 140, 179, 264, 265, 819, 1320
MunI	CAATTG	1 cut(s)	1215
Mva1269I	GAATGC	1 cut(s)	445
MvaI	CCWGG	5 cut(s)	22, 140, 179, 819, 1320
MvnI	CGCG	4 cut(s)	288, 383, 764, 1070
MwoI	GCNNNNNNNGC	6 cut(s)	25, 480, 770, 874, 930, 939
NaeI	GCCGGC	1 cut(s)	771
NarI	GGCGCC	1 cut(s)	767
NciI	CCSGG	2 cut(s)	264, 265
NdeI	CATATG	1 cut(s)	1198
NdeII	GATC	9 cut(s)	50, 135, 296, 827, 913, 952, 1192, 1492, 1546
NgoMIV	GCCGGC	1 cut(s)	769
NlaIII	CATG	3 cut(s)	125, 337, 1030
NlaIV	GGNNCC	5 cut(s)	137, 242, 417, 768, 793
NmeAIII	GCCGAG	1 cut(s)	1246
NmuCI	GTSAC	3 cut(s)	268, 409, 837
NruI	TCGCGA	1 cut(s)	288
NspI	RCATGY	2 cut(s)	337, 1030
NspV	TTCGAA	1 cut(s)	880
PaeR7I	CTCGAG	2 cut(s)	323, 1076
PceI	AGGCCT	2 cut(s)	1160, 1368
PciI	ACATGT	1 cut(s)	333
PciSI	GCTCTTC	1 cut(s)	558
PcsI	WCGNNNNNNNCGW	6 cut(s)	213, 231, 285, 623, 865, 1074
PctI	GAATGC	1 cut(s)	445
PdiI	GCCGGC	1 cut(s)	771
PfeI	GAWTC	4 cut(s)	184, 201, 356, 1087
PflMI	CCANNNNNTGG	1 cut(s)	1063
PfoI	TCCNGGA	3 cut(s)	138, 177, 817
Ple19I	CGATCG	1 cut(s)	916
PleI	GAGTC	8 cut(s)	355, 520, 622, 660, 741, 743, 820, 855
PluTI	GGCGCC	1 cut(s)	770
PpsI	GAGTC	8 cut(s)	355, 520, 622, 660, 741, 743, 820, 855
PpuMI	RGGWCCY	1 cut(s)	240
PscI	ACATGT	1 cut(s)	333
PshAI	GACNNNNGTC	2 cut(s)	734, 1071
Psp124BI	GAGCTC	4 cut(s)	147, 324, 330, 570
Psp5II	RGGWCCY	1 cut(s)	240
Psp6I	CCWGG	5 cut(s)	20, 138, 177, 817, 1318
PspEI	GGTNACC	1 cut(s)	268
PspFI	CCCAGC	2 cut(s)	716, 739
PspGI	CCWGG	5 cut(s)	20, 138, 177, 817, 1318
PspN4I	GGNNCC	5 cut(s)	137, 242, 417, 768, 793
PspPI	GGNCC	9 cut(s)	7, 156, 175, 240, 792, 821, 909, 1316, 1518
PspPPI	RGGWCCY	1 cut(s)	240
PspXI	VCTCGAGB	1 cut(s)	323
PstI	CTGCAG	3 cut(s)	788, 890, 1050
PsuI	RGATCY	3 cut(s)	135, 827, 952
PvuI	CGATCG	1 cut(s)	916
PvuII	CAGCTG	1 cut(s)	1304
RruI	TCGCGA	1 cut(s)	288
RsaI	GTAC	1 cut(s)	1241
RsaNI	GTAC	1 cut(s)	1240
Rsr2I	CGGWCCG	1 cut(s)	156
RsrII	CGGWCCG	1 cut(s)	156
SacI	GAGCTC	4 cut(s)	147, 324, 330, 570
SacII	CCGCGG	1 cut(s)	765
SapI	GCTCTTC	1 cut(s)	558
SaqAI	TTAA	5 cut(s)	1235, 1448, 1481, 1499, 1599
Sau3AI	GATC	9 cut(s)	50, 135, 296, 827, 913, 952, 1192, 1492, 1546
Sau96I	GGNCC	9 cut(s)	7, 156, 175, 240, 792, 821, 909, 1316, 1518
SchI	GAGTC	8 cut(s)	355, 521, 622, 661, 741, 744, 821, 856
ScrFI	CCNGG	7 cut(s)	22, 140, 179, 264, 265, 819, 1320
SduI	GDGCHC	5 cut(s)	147, 324, 330, 570, 1112
SexAI	ACCWGGT	1 cut(s)	1318
SfaNI	GCATC	1 cut(s)	498
SfcI	CTRYAG	3 cut(s)	784, 886, 1046
SfoI	GGCGCC	1 cut(s)	768
Sfr274I	CTCGAG	2 cut(s)	323, 1076
Sfr303I	CCGCGG	1 cut(s)	765
SfuI	TTCGAA	1 cut(s)	880
SgrAI	CRCCGGYG	1 cut(s)	769
SgrBI	CCGCGG	1 cut(s)	765
SinI	GGWCC	8 cut(s)	7, 156, 175, 240, 821, 909, 1316, 1518
SlaI	CTCGAG	2 cut(s)	323, 1076
SmaI	CCCGGG	1 cut(s)	265
SmlI	CTYRAG	4 cut(s)	323, 528, 863, 1076
SmoI	CTYRAG	4 cut(s)	323, 528, 863, 1076
Sse9I	AATT	7 cut(s)	305, 458, 1215, 1383, 1445, 1536, 1577
SseBI	AGGCCT	2 cut(s)	1160, 1368
SspDI	GGCGCC	1 cut(s)	766
SspMI	CTAG	3 cut(s)	644, 693, 1524
SstI	GAGCTC	4 cut(s)	147, 324, 330, 570
StuI	AGGCCT	2 cut(s)	1160, 1368
StyD4I	CCNGG	7 cut(s)	20, 138, 177, 262, 263, 817, 1318
StyI	CCWWGG	3 cut(s)	905, 946, 1340
TaaI	ACNGT	5 cut(s)	40, 415, 619, 1001, 1637
TaiI	ACGT	1 cut(s)	314
TaqII	GACCGA	2 cut(s)	144, 838
TasI	AATT	7 cut(s)	305, 458, 1215, 1383, 1445, 1536, 1577
TatI	WGTACW	1 cut(s)	1239
TauI	GCSGC	4 cut(s)	486, 489, 626, 767
TfiI	GAWTC	4 cut(s)	184, 201, 356, 1087
Tru1I	TTAA	5 cut(s)	1235, 1448, 1481, 1499, 1599
Tru9I	TTAA	5 cut(s)	1235, 1448, 1481, 1499, 1599
TscAI	CASTG	2 cut(s)	793, 944
TseFI	GTSAC	3 cut(s)	268, 409, 837
TseI	GCWGC	7 cut(s)	349, 585, 780, 783, 885, 1045, 1304
Tsp45I	GTSAC	3 cut(s)	268, 409, 837
TspDTI	ATGAA	2 cut(s)	17, 1458
TspGWI	ACGGA	2 cut(s)	462, 1483
TspMI	CCCGGG	1 cut(s)	263
TspRI	CASTG	2 cut(s)	793, 944
Van91I	CCANNNNNTGG	1 cut(s)	1063
VpaK11BI	GGWCC	8 cut(s)	7, 156, 175, 240, 821, 909, 1316, 1518
XceI	RCATGY	2 cut(s)	337, 1030
XcmI	CCANNNNNNNNNTGG	1 cut(s)	554
XhoI	CTCGAG	2 cut(s)	323, 1076
XmaI	CCCGGG	1 cut(s)	263
XspI	CTAG	3 cut(s)	644, 693, 1524

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.