MD13G1194500.v1.1

heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007781
Physical Location & Seq
Reverse (-)
16832841 .. 16833707
867 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1194500.v1.1.491

Sequence Viewer

Length: 777 bp
ATGATTCCTTCTTTTGGTAAAAACAACAACCCAATCAGGATTTTGATTCCCACCCAAGATTGGCCTGCCTTTTTCACAAGAAGGGCATCTTTCATCTTTCTATTAGTCTTGTCGCATCCCTCTATTTCTTCTGCAGCTCCACTACCTCCGCCACCGCCGCCCCAGCAGCTGCCACTGAAGCCGCCAACCATGAAGGTCCACCCCATGCCCAGGAAGCGCAACATCACCGTCCAATACAGATCAAGAAACCCACTTTCTGAAGCGCAGGCACTTTTGGGTCTGACCCATAAGAAGCTCCGCCGTCTCCCCCATGTCTTCAGCCGGATCCTGGAGCTCCCCTTTCGGTCCGACGCTGACGTTCTGGTCCAGGAGAATCCCGATTGCTTCCGATTCGTCGCCGAAGCCGATAATATCGGCGACGGCGTGAGGGCCCACACCATCGAAATCCATCCCGGGGTGACCAAAATCGTGGTTCGCGAAAGTGGATCGGCGGAATTGACGATGGACGAGCTCGAGCTCGACATGTGGAGGTTTCGGCTGCCGGAATCGACTCGACCCGAGTTGGCCAGCGCGGTTTTCGTGGACGGGGAGCTTATCGTGACGGTGCCGAAGGATGTGGGAGTGGAGAATGCGGACGGGGGTGATGGTGGCGGAGAGGTTTGGGGCGGTGGCGACGGGAATGGGGGTGGGGGTTTCAGAGGAGGTATGGTTGGATTTAGCTTTGCAACTGAGCTTTTGAGGCTGCCGTTGTTTTATGCTGCTGTTCTTGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

28.3

Weight (kDa)

6.32

Isoelectric Point (pI)

50.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 604
AccII CGCG 2 cut(s) 477, 572
AclWI GGATC 3 cut(s) 319, 332, 493
AcoI YGGCCR 1 cut(s) 564
AcuI CTGAAG 3 cut(s) 197, 279, 301
AfiI CCNNNNNNNGG 5 cut(s) 14, 60, 210, 328, 454
AflIII ACRYGT 1 cut(s) 522
AgsI TTSAA 1 cut(s) 773
AjnI CCWGG 3 cut(s) 209, 327, 366
AluBI AGCT 9 cut(s) 137, 169, 295, 334, 511, 517, 592, 720, 733
AluI AGCT 9 cut(s) 137, 169, 295, 334, 511, 517, 592, 720, 733
Alw21I GWGCWC 3 cut(s) 336, 513, 519
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 3 cut(s) 319, 332, 493
AlwNI CAGNNNCTG 1 cut(s) 169
Ama87I CYCGRG 3 cut(s) 452, 512, 557
AoxI GGCC 3 cut(s) 62, 429, 564
ApaI GGGCCC 1 cut(s) 433
ApeKI GCWGC 6 cut(s) 134, 166, 169, 538, 742, 758
AspLEI GCGC 3 cut(s) 219, 265, 572
AspS9I GGNCC 5 cut(s) 196, 345, 364, 429, 430
AsuC2I CCSGG 2 cut(s) 453, 454
AsuHPI GGTGA 3 cut(s) 217, 469, 653
AvaI CYCGRG 3 cut(s) 452, 512, 557
AvaII GGWCC 3 cut(s) 196, 345, 364
BaeGI GKGCMC 1 cut(s) 433
BalI TGGCCA 1 cut(s) 566
BamHI GGATCC 1 cut(s) 324
BanI GGYRCC 1 cut(s) 604
BanII GRGCYC 4 cut(s) 336, 433, 513, 519
BbsI GAAGAC 1 cut(s) 307
Bbv12I GWGCWC 3 cut(s) 336, 513, 519
BbvI GCAGC 6 cut(s) 146, 156, 178, 525, 729, 745
BccI CCATC 4 cut(s) 446, 456, 496, 638
BceAI ACGGC 3 cut(s) 285, 436, 730
BciT130I CCWGG 3 cut(s) 211, 329, 368
BcnI CCSGG 2 cut(s) 453, 454
BcoDI GTCTC 1 cut(s) 308
BfmI CTRYAG 1 cut(s) 132
BisI GCNGC 8 cut(s) 135, 158, 167, 170, 182, 539, 743, 759
BlsI GCNGC 8 cut(s) 136, 159, 168, 171, 183, 540, 744, 760
Bme1390I CCNGG 5 cut(s) 211, 329, 368, 453, 454
Bme18I GGWCC 3 cut(s) 196, 345, 364
BmeT110I CYCGRG 3 cut(s) 452, 512, 557
BmgT120I GGNCC 5 cut(s) 196, 345, 364, 429, 430
BmiI GGNNCC 3 cut(s) 326, 431, 606
BmrFI CCNGG 5 cut(s) 211, 329, 368, 453, 454
BmsI GCATC 2 cut(s) 95, 124
BpiI GAAGAC 1 cut(s) 307
BpmI CTGGAG 1 cut(s) 350
BpuMI CCSGG 2 cut(s) 453, 454
BsaJI CCNNGG 3 cut(s) 209, 452, 453
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bsc4I CCNNNNNNNGG 5 cut(s) 14, 60, 210, 328, 454
BseBI CCWGG 3 cut(s) 211, 329, 368
BseDI CCNNGG 3 cut(s) 209, 452, 453
BseGI GGATG 3 cut(s) 115, 448, 619
BseLI CCNNNNNNNGG 5 cut(s) 14, 60, 210, 328, 454
BseMII CTCAG 1 cut(s) 720
BseRI GAGGAG 1 cut(s) 714
BseSI GKGCMC 1 cut(s) 433
BseXI GCAGC 6 cut(s) 146, 156, 178, 525, 729, 745
BseYI CCCAGC 1 cut(s) 162
Bsh1236I CGCG 2 cut(s) 477, 572
BshFI GGCC 3 cut(s) 64, 431, 566
BshNI GGYRCC 1 cut(s) 604
BsiHKAI GWGCWC 3 cut(s) 336, 513, 519
BsiHKCI CYCGRG 3 cut(s) 452, 512, 557
BsiSI CCGG 3 cut(s) 322, 453, 542
BslI CCNNNNNNNGG 5 cut(s) 14, 60, 210, 328, 454
BsmAI GTCTC 1 cut(s) 308
BsmBI CGTCTC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 634
BsnI GGCC 3 cut(s) 64, 431, 566
BsoBI CYCGRG 3 cut(s) 452, 512, 557
Bsp120I GGGCCC 1 cut(s) 429
Bsp1286I GDGCHC 4 cut(s) 336, 433, 513, 519
Bsp143I GATC 3 cut(s) 239, 324, 485
Bsp68I TCGCGA 1 cut(s) 477
BspANI GGCC 3 cut(s) 64, 431, 566
BspCNI CTCAG 1 cut(s) 721
BspFNI CGCG 2 cut(s) 477, 572
BspLI GGNNCC 3 cut(s) 326, 431, 606
BspMAI CTGCAG 1 cut(s) 136
BspPI GGATC 3 cut(s) 319, 332, 493
BspT107I GGYRCC 1 cut(s) 604
BssECI CCNNGG 3 cut(s) 209, 452, 453
BssMI GATC 3 cut(s) 239, 324, 485
Bst2UI CCWGG 3 cut(s) 211, 329, 368
Bst4CI ACNGT 2 cut(s) 229, 604
BstC8I GCNNGC 3 cut(s) 66, 267, 568
BstDEI CTNAG 1 cut(s) 729
BstEII GGTNACC 1 cut(s) 457
BstF5I GGATG 3 cut(s) 115, 448, 619
BstFNI CGCG 2 cut(s) 477, 572
BstHHI GCGC 3 cut(s) 219, 265, 572
BstKTI GATC 3 cut(s) 242, 327, 488
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 3 cut(s) 239, 324, 485
BstMWI GCNNNNNNNGC 6 cut(s) 157, 163, 166, 178, 214, 739
BstNI CCWGG 3 cut(s) 211, 329, 368
BstNSI RCATGY 1 cut(s) 526
BstPI GGTNACC 1 cut(s) 457
BstSCI CCNGG 5 cut(s) 209, 327, 366, 451, 452
BstSFI CTRYAG 1 cut(s) 132
BstSLI GKGCMC 1 cut(s) 433
BstUI CGCG 2 cut(s) 477, 572
BstV1I GCAGC 6 cut(s) 146, 156, 178, 525, 729, 745
BstV2I GAAGAC 1 cut(s) 307
BstX2I RGATCY 1 cut(s) 324
BstXI CCANNNNNNTGG 1 cut(s) 469
BstYI RGATCY 1 cut(s) 324
BsuRI GGCC 3 cut(s) 64, 431, 566
BtsCI GGATG 3 cut(s) 115, 448, 619
BtsIMutI CAGTG 1 cut(s) 173
BtuMI TCGCGA 1 cut(s) 477
Cac8I GCNNGC 3 cut(s) 66, 267, 568
CaiI CAGNNNCTG 1 cut(s) 169
CfoI GCGC 3 cut(s) 219, 265, 572
Cfr13I GGNCC 5 cut(s) 196, 345, 364, 429, 430
Cfr9I CCCGGG 1 cut(s) 452
CpoI CGGWCCG 1 cut(s) 345
CseI GACGC 1 cut(s) 359
CspI CGGWCCG 1 cut(s) 345
CviAII CATG 4 cut(s) 190, 205, 311, 523
DdeI CTNAG 1 cut(s) 729
DpnI GATC 3 cut(s) 241, 326, 487
DpnII GATC 3 cut(s) 239, 324, 485
DrdI GACNNNNNNGTC 1 cut(s) 362
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 1 cut(s) 564
EciI GGCGGA 4 cut(s) 138, 287, 506, 666
Ecl136II GAGCTC 3 cut(s) 334, 511, 517
Eco24I GRGCYC 4 cut(s) 336, 433, 513, 519
Eco47I GGWCC 3 cut(s) 196, 345, 364
Eco53kI GAGCTC 3 cut(s) 334, 511, 517
Eco57I CTGAAG 3 cut(s) 197, 279, 301
Eco88I CYCGRG 3 cut(s) 452, 512, 557
Eco91I GGTNACC 1 cut(s) 457
EcoICRI GAGCTC 3 cut(s) 334, 511, 517
EcoO109I RGGNCCY 1 cut(s) 429
EcoO65I GGTNACC 1 cut(s) 457
EcoRII CCWGG 3 cut(s) 209, 327, 366
EcoT38I GRGCYC 4 cut(s) 336, 433, 513, 519
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 4 cut(s) 193, 208, 314, 526
FaiI YATR 7 cut(s) 191, 206, 288, 312, 524, 707, 756
FalI AAGNNNNNCTT 2 cut(s) 73, 105
FatI CATG 4 cut(s) 189, 204, 310, 522
Fnu4HI GCNGC 8 cut(s) 135, 158, 167, 170, 182, 539, 743, 759
FokI GGATG 3 cut(s) 102, 435, 626
FriOI GRGCYC 4 cut(s) 336, 433, 513, 519
Fsp4HI GCNGC 8 cut(s) 135, 158, 167, 170, 182, 539, 743, 759
GlaI GCGC 3 cut(s) 218, 264, 571
GluI GCNGC 8 cut(s) 135, 158, 167, 170, 182, 539, 743, 759
GsaI CCCAGC 1 cut(s) 166
GsuI CTGGAG 1 cut(s) 350
HaeIII GGCC 3 cut(s) 64, 431, 566
HapII CCGG 3 cut(s) 322, 453, 542
HgaI GACGC 1 cut(s) 359
HhaI GCGC 3 cut(s) 219, 265, 572
Hin1II CATG 4 cut(s) 193, 208, 314, 526
Hin6I GCGC 3 cut(s) 217, 263, 570
HinP1I GCGC 3 cut(s) 217, 263, 570
HinfI GANTC 6 cut(s) 4, 46, 373, 390, 545, 550
HpaII CCGG 3 cut(s) 322, 453, 542
HphI GGTGA 3 cut(s) 217, 469, 653
Hpy166II GTNNAC 2 cut(s) 199, 583
Hpy188I TCNGA 5 cut(s) 259, 282, 349, 389, 698
Hpy188III TCNNGA 5 cut(s) 37, 243, 377, 476, 598
Hpy8I GTNNAC 2 cut(s) 199, 583
Hpy99I CGWCG 4 cut(s) 353, 398, 422, 677
HpyAV CCTTC 4 cut(s) 18, 75, 187, 604
HpyCH4III ACNGT 2 cut(s) 229, 604
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 2 cut(s) 134, 725
HpyF10VI GCNNNNNNNGC 6 cut(s) 157, 163, 166, 178, 214, 739
HpyF3I CTNAG 1 cut(s) 729
HpySE526I ACGT 1 cut(s) 357
Hsp92II CATG 4 cut(s) 193, 208, 314, 526
HspAI GCGC 3 cut(s) 217, 263, 570
Kzo9I GATC 3 cut(s) 239, 324, 485
LmnI GCTCC 5 cut(s) 142, 300, 331, 339, 589
Lsp1109I GCAGC 6 cut(s) 146, 156, 178, 525, 729, 745
LweI GCATC 2 cut(s) 95, 124
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 2 cut(s) 457, 598
MalI GATC 3 cut(s) 241, 326, 487
MboI GATC 3 cut(s) 239, 324, 485
MboII GAAGA 2 cut(s) 120, 307
MflI RGATCY 1 cut(s) 324
MhlI GDGCHC 4 cut(s) 336, 433, 513, 519
MlsI TGGCCA 1 cut(s) 566
MluCI AATT 1 cut(s) 494
MluNI TGGCCA 1 cut(s) 566
MlyI GAGTC 1 cut(s) 544
MmeI TCCRAC 2 cut(s) 372, 691
MnlI CCTC 8 cut(s) 130, 156, 420, 522, 649, 692, 695, 732
Mox20I TGGCCA 1 cut(s) 566
MscI TGGCCA 1 cut(s) 566
Msp20I TGGCCA 1 cut(s) 566
MspA1I CMGCKG 1 cut(s) 169
MspI CCGG 3 cut(s) 322, 453, 542
MspR9I CCNGG 5 cut(s) 211, 329, 368, 453, 454
Mva1269I GAATGC 1 cut(s) 634
MvaI CCWGG 3 cut(s) 211, 329, 368
MvnI CGCG 2 cut(s) 477, 572
MwoI GCNNNNNNNGC 6 cut(s) 157, 163, 166, 178, 214, 739
NciI CCSGG 2 cut(s) 453, 454
NdeII GATC 3 cut(s) 239, 324, 485
NlaIII CATG 4 cut(s) 193, 208, 314, 526
NlaIV GGNNCC 3 cut(s) 326, 431, 606
NmuCI GTSAC 2 cut(s) 457, 598
NruI TCGCGA 1 cut(s) 477
NspI RCATGY 1 cut(s) 526
PaeR7I CTCGAG 1 cut(s) 512
PciI ACATGT 1 cut(s) 522
PcsI WCGNNNNNNNCGW 3 cut(s) 402, 420, 474
PctI GAATGC 1 cut(s) 634
PfeI GAWTC 5 cut(s) 4, 46, 373, 390, 545
PfoI TCCNGGA 2 cut(s) 327, 366
PkrI GCNGC 8 cut(s) 136, 159, 168, 171, 183, 540, 744, 760
PleI GAGTC 1 cut(s) 544
PpsI GAGTC 1 cut(s) 544
PscI ACATGT 1 cut(s) 522
Psp124BI GAGCTC 3 cut(s) 336, 513, 519
Psp6I CCWGG 3 cut(s) 209, 327, 366
PspEI GGTNACC 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 162
PspGI CCWGG 3 cut(s) 209, 327, 366
PspN4I GGNNCC 3 cut(s) 326, 431, 606
PspOMI GGGCCC 1 cut(s) 429
PspPI GGNCC 5 cut(s) 196, 345, 364, 429, 430
PspXI VCTCGAGB 1 cut(s) 512
PstI CTGCAG 1 cut(s) 136
PstNI CAGNNNCTG 1 cut(s) 169
PsuI RGATCY 1 cut(s) 324
PvuII CAGCTG 1 cut(s) 169
RruI TCGCGA 1 cut(s) 477
Rsr2I CGGWCCG 1 cut(s) 345
RsrII CGGWCCG 1 cut(s) 345
SacI GAGCTC 3 cut(s) 336, 513, 519
SatI GCNGC 8 cut(s) 135, 158, 167, 170, 182, 539, 743, 759
Sau3AI GATC 3 cut(s) 239, 324, 485
Sau96I GGNCC 5 cut(s) 196, 345, 364, 429, 430
SchI GAGTC 1 cut(s) 544
ScrFI CCNGG 5 cut(s) 211, 329, 368, 453, 454
SduI GDGCHC 4 cut(s) 336, 433, 513, 519
SfaNI GCATC 2 cut(s) 95, 124
SfcI CTRYAG 1 cut(s) 132
Sfr274I CTCGAG 1 cut(s) 512
SinI GGWCC 3 cut(s) 196, 345, 364
SlaI CTCGAG 1 cut(s) 512
SmaI CCCGGG 1 cut(s) 454
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 1 cut(s) 494
SstI GAGCTC 3 cut(s) 336, 513, 519
StyD4I CCNGG 5 cut(s) 209, 327, 366, 451, 452
TaaI ACNGT 2 cut(s) 229, 604
TaiI ACGT 1 cut(s) 360
TaqI TCGA 5 cut(s) 441, 513, 519, 548, 553
TaqII GACCGA 1 cut(s) 333
TasI AATT 1 cut(s) 494
TauI GCSGC 2 cut(s) 160, 184
TfiI GAWTC 5 cut(s) 4, 46, 373, 390, 545
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 2 cut(s) 457, 598
TseI GCWGC 6 cut(s) 134, 166, 169, 538, 742, 758
Tsp45I GTSAC 2 cut(s) 457, 598
TspDTI ATGAA 2 cut(s) 82, 206
TspMI CCCGGG 1 cut(s) 452
TspRI CASTG 1 cut(s) 180
VpaK11BI GGWCC 3 cut(s) 196, 345, 364
XceI RCATGY 1 cut(s) 526
XhoI CTCGAG 1 cut(s) 512
XmaI CCCGGG 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.