AT3G54570

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003606
Physical Location & Seq
Reverse (-)
20197949 .. 20199202
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54570.1

Sequence Viewer

Length: 1254 bp
ATGGTTCAGAGTAAAACTGCTCAAGCTTTGGTAGGGCAAGGACATGGTAGGAGGCTTAGACCGGAGATGATAAAGAAGAGACCAAGGGCTAGTAGGCTTCCTCAACCAACGACACCGGAGAAGCAGATCGTTGCTAAAGTCACAGGCGGGTCACCTAATTATATGAAGGGAACGAGGAGTTCAGAGGCTAGAAGACAGAGCCAAAGTGTTCAAGCTGGTTTAGACAAGAAGAGCCAAACCGGTAAGAAACTTGATTCTTGCTCTAGAGAAAAGAAACAGAGTAGTAGTAGGTCTTTGAAAAAGGGTCAGAGCTTCAAGAGAAGTGGTAGGATTGGTCGTTGTTGGGATGCGAATGTGCATAGAGCAACTTGCTCTTCGCTTCTCAAAAACTCAAAATTCACAGAGGATCTGATGTTTACTTCTCCTCATATCTTGAAAGTCTGTCCTTATACGTATTGTTCACTCAACGCGCATCTTCACAGCCAATTCCCACCTCTTCTAAGCTTTATATCCGAAAGGAGACGCTCCTTGAAGTCCCATGCAAGTGGTGAATGTGATGATGGTTGTGTGGAAATCTATGTTGATGAGAAGAAAGAAAATAGAAGCACTCGAGAAACTGATATCAAAGTAATCGATTCTGAGGCTGAGAATATAGAGATGGAATTGGGAGAGGTTAAAGATTTAGATTCTGAGAGTGCAGAGATAGTGAGCTTACTAGAAGGTGAAGGCATTGAATCATGTAACTTTGCAGTTCTTGAACAATCAGAAAATTCAAGTGAGGATCAAGAGCGTGAAGAGGGTGGTTTCTCGAATAACACAACCAACTCTCTTTTATTTGAACAGTCGATTATACAAGATGACATCATTCTAGGGAATGCTGTGGATGAGAAGCATGAATCTAAGGAAGCTGAAGACTGGAAGGAGGCAGATGGAGAGAAAGTGAAAGAGCGGATTAAACTTGTATTGAAAACAGAGGAAGCTCTGTTATCACTTGCACAAAAACCTTGCAACCGAGAAGAGTGCATTGAGGATTGTAGGAGACTCAACCCAAGAGAACCCAATTACATTCAAACTACTGTAGAGCCGAGCAACGAAACAGTTGATCTCAGGCATCAGGACATGGATGAGAGGAAGAAAGCAGAGGAGTGGATGATTGATTATGCTCTGCAACACACCGTAAGTAAACTTGTTGTAGAAAGGAAGAAGGATGTGGCATTGCTCGTTGAGGCATTTGAAACAACAGTACCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

417

Amino Acids

47.21

Weight (kDa)

5.99

Isoelectric Point (pI)

65.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 298 - 416 4.8e-32 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 949
AccII CGCG 1 cut(s) 470
AciI CCGC 2 cut(s) 147, 949
AclWI GGATC 2 cut(s) 414, 789
AcsI RAATTY 2 cut(s) 395, 769
AcuI CTGAAG 1 cut(s) 930
AfaI GTAC 1 cut(s) 1245
AgeI ACCGGT 1 cut(s) 239
AluBI AGCT 7 cut(s) 26, 215, 312, 504, 711, 908, 980
AluI AGCT 7 cut(s) 26, 215, 312, 504, 711, 908, 980
Alw26I GTCTC 3 cut(s) 73, 514, 1033
AlwI GGATC 2 cut(s) 414, 789
Ama87I CYCGRG 1 cut(s) 609
ApoI RAATTY 2 cut(s) 395, 769
AsiGI ACCGGT 1 cut(s) 239
AspLEI GCGC 1 cut(s) 472
AsuHPI GGTGA 3 cut(s) 144, 560, 734
AvaI CYCGRG 1 cut(s) 609
BaeI ACNNNNGTAYC 1 cut(s) 1227
BbsI GAAGAC 2 cut(s) 199, 918
BccI CCATC 3 cut(s) 554, 652, 923
BcgI CGANNNNNNTGC 2 cut(s) 1002, 1036
BcoDI GTCTC 3 cut(s) 73, 514, 1033
BfaI CTAG 5 cut(s) 90, 189, 264, 716, 869
BfmI CTRYAG 1 cut(s) 1077
BmeT110I CYCGRG 1 cut(s) 609
BmsI GCATC 3 cut(s) 337, 481, 1120
BpiI GAAGAC 2 cut(s) 199, 918
BpuEI CTTGAG 1 cut(s) 6
Bsa29I ATCGAT 1 cut(s) 633
BsaAI YACGTR 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 73
BsaJI CCNNGG 1 cut(s) 83
BsaWI WCCGGW 3 cut(s) 61, 115, 239
BsaXI ACNNNNNCTCC 2 cut(s) 924, 954
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 920
Bse3DI GCAATG 1 cut(s) 1214
BseCI ATCGAT 1 cut(s) 633
BseDI CCNNGG 1 cut(s) 83
BseGI GGATG 5 cut(s) 352, 889, 1129, 1155, 1213
BseMI GCAATG 1 cut(s) 1214
BseMII CTCAG 4 cut(s) 630, 636, 681, 1120
BseNI ACTGG 1 cut(s) 920
BseRI GAGGAG 3 cut(s) 190, 414, 1157
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 1 cut(s) 470
BshTI ACCGGT 1 cut(s) 239
BshVI ATCGAT 1 cut(s) 633
BsiHKCI CYCGRG 1 cut(s) 609
BsiSI CCGG 3 cut(s) 62, 116, 240
BslFI GGGAC 1 cut(s) 520
BsmAI GTCTC 3 cut(s) 73, 514, 1033
BsmBI CGTCTC 1 cut(s) 514
BsmFI GGGAC 1 cut(s) 520
BsmI GAATGC 1 cut(s) 880
Bso31I GGTCTC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 609
Bsp143I GATC 4 cut(s) 126, 406, 781, 1102
BspACI CCGC 2 cut(s) 147, 949
BspCNI CTCAG 4 cut(s) 631, 637, 682, 1119
BspDI ATCGAT 1 cut(s) 633
BspFNI CGCG 1 cut(s) 470
BspPI GGATC 2 cut(s) 414, 789
BspQI GCTCTTC 2 cut(s) 224, 379
BspTNI GGTCTC 1 cut(s) 73
BsrBI CCGCTC 1 cut(s) 949
BsrDI GCAATG 1 cut(s) 1214
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 1 cut(s) 920
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 83
BssMI GATC 4 cut(s) 126, 406, 781, 1102
BssT1I CCWWGG 1 cut(s) 83
Bst4CI ACNGT 5 cut(s) 843, 1078, 1099, 1177, 1243
Bst6I CTCTTC 6 cut(s) 71, 224, 379, 501, 789, 1011
BstBAI YACGTR 1 cut(s) 453
BstDEI CTNAG 7 cut(s) 56, 500, 639, 645, 690, 900, 1106
BstEII GGTNACC 1 cut(s) 150
BstF5I GGATG 5 cut(s) 352, 889, 1129, 1155, 1213
BstFNI CGCG 1 cut(s) 470
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 4 cut(s) 129, 409, 784, 1105
BstMAI GTCTC 3 cut(s) 73, 514, 1033
BstMBI GATC 4 cut(s) 126, 406, 781, 1102
BstPI GGTNACC 1 cut(s) 150
BstSFI CTRYAG 1 cut(s) 1077
BstSNI TACGTA 1 cut(s) 453
BstUI CGCG 1 cut(s) 470
BstV2I GAAGAC 2 cut(s) 199, 918
BstX2I RGATCY 1 cut(s) 406
BstXI CCANNNNNNTGG 1 cut(s) 545
BstYI RGATCY 1 cut(s) 406
Bsu15I ATCGAT 1 cut(s) 633
BsuTUI ATCGAT 1 cut(s) 633
BtsCI GGATG 5 cut(s) 352, 889, 1129, 1155, 1213
CfoI GCGC 1 cut(s) 472
Cfr10I RCCGGY 1 cut(s) 239
ClaI ATCGAT 1 cut(s) 633
CseI GACGC 1 cut(s) 531
Csp6I GTAC 1 cut(s) 1244
CspAI ACCGGT 1 cut(s) 239
CspCI CAANNNNNGTGG 2 cut(s) 304, 339
CviAII CATG 5 cut(s) 44, 539, 738, 893, 1120
CviQI GTAC 1 cut(s) 1244
DdeI CTNAG 7 cut(s) 56, 500, 639, 645, 690, 900, 1106
DpnI GATC 4 cut(s) 128, 408, 783, 1104
DpnII GATC 4 cut(s) 126, 406, 781, 1102
Eam1104I CTCTTC 6 cut(s) 71, 224, 379, 501, 789, 1011
EarI CTCTTC 6 cut(s) 71, 224, 379, 501, 789, 1011
Eco105I TACGTA 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 83
Eco31I GGTCTC 1 cut(s) 73
Eco32I GATATC 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 930
Eco88I CYCGRG 1 cut(s) 609
Eco91I GGTNACC 1 cut(s) 150
EcoO65I GGTNACC 1 cut(s) 150
EcoRV GATATC 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 83
Esp3I CGTCTC 1 cut(s) 514
FaeI CATG 5 cut(s) 47, 542, 741, 896, 1123
FaqI GGGAC 1 cut(s) 520
FatI CATG 5 cut(s) 43, 538, 737, 892, 1119
FauI CCCGC 1 cut(s) 140
FokI GGATG 5 cut(s) 359, 896, 1136, 1162, 1220
FspBI CTAG 5 cut(s) 90, 189, 264, 716, 869
GlaI GCGC 1 cut(s) 471
HapII CCGG 3 cut(s) 62, 116, 240
HgaI GACGC 1 cut(s) 531
HhaI GCGC 1 cut(s) 472
Hin1II CATG 5 cut(s) 47, 542, 741, 896, 1123
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HindIII AAGCTT 2 cut(s) 24, 502
HinfI GANTC 6 cut(s) 254, 635, 686, 734, 896, 1041
HpaII CCGG 3 cut(s) 62, 116, 240
HphI GGTGA 3 cut(s) 144, 560, 734
Hpy166II GTNNAC 3 cut(s) 417, 461, 1184
Hpy188I TCNGA 8 cut(s) 9, 184, 309, 411, 514, 640, 691, 766
Hpy188III TCNNGA 8 cut(s) 264, 316, 433, 611, 755, 785, 808, 1115
Hpy8I GTNNAC 3 cut(s) 417, 461, 1184
HpyAV CCTTC 5 cut(s) 160, 713, 719, 913, 1198
HpyCH4III ACNGT 5 cut(s) 843, 1078, 1099, 1177, 1243
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 8 cut(s) 358, 542, 698, 749, 995, 1008, 1023, 1168
HpyF3I CTNAG 7 cut(s) 56, 500, 639, 645, 690, 900, 1106
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 5 cut(s) 47, 542, 741, 896, 1123
HspAI GCGC 1 cut(s) 470
Kzo9I GATC 4 cut(s) 126, 406, 781, 1102
LguI GCTCTTC 2 cut(s) 224, 379
LmnI GCTCC 1 cut(s) 530
LpnPI CCDG 8 cut(s) 75, 129, 129, 201, 253, 901, 1093, 1100
LweI GCATC 3 cut(s) 337, 481, 1120
MaeI CTAG 5 cut(s) 90, 189, 264, 716, 869
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 3 cut(s) 139, 150, 740
MalI GATC 4 cut(s) 128, 408, 783, 1104
MbiI CCGCTC 1 cut(s) 949
MboI GATC 4 cut(s) 126, 406, 781, 1102
MflI RGATCY 1 cut(s) 406
MluCI AATT 6 cut(s) 157, 395, 485, 662, 769, 1060
MlyI GAGTC 1 cut(s) 1035
MseI TTAA 2 cut(s) 675, 954
MslI CAYNNNNRTG 1 cut(s) 543
MspI CCGG 3 cut(s) 62, 116, 240
Mva1269I GAATGC 1 cut(s) 880
MvnI CGCG 1 cut(s) 470
NdeII GATC 4 cut(s) 126, 406, 781, 1102
NlaIII CATG 5 cut(s) 47, 542, 741, 896, 1123
NmeAIII GCCGAG 1 cut(s) 1110
NmuCI GTSAC 2 cut(s) 139, 150
PaeR7I CTCGAG 1 cut(s) 609
PciSI GCTCTTC 2 cut(s) 224, 379
PctI GAATGC 1 cut(s) 880
PfeI GAWTC 5 cut(s) 254, 635, 686, 734, 896
PinAI ACCGGT 1 cut(s) 239
PleI GAGTC 1 cut(s) 1035
PpsI GAGTC 1 cut(s) 1035
Ppu21I YACGTR 1 cut(s) 453
PspEI GGTNACC 1 cut(s) 150
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 1 cut(s) 1245
RsaNI GTAC 1 cut(s) 1244
RseI CAYNNNNRTG 1 cut(s) 543
SapI GCTCTTC 2 cut(s) 224, 379
SaqAI TTAA 2 cut(s) 675, 954
Sau3AI GATC 4 cut(s) 126, 406, 781, 1102
SchI GAGTC 1 cut(s) 1035
SfaNI GCATC 3 cut(s) 337, 481, 1120
SfcI CTRYAG 1 cut(s) 1077
Sfr274I CTCGAG 1 cut(s) 609
SlaI CTCGAG 1 cut(s) 609
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 2 cut(s) 21, 609
SmoI CTYRAG 2 cut(s) 21, 609
SnaBI TACGTA 1 cut(s) 453
Sse9I AATT 6 cut(s) 157, 395, 485, 662, 769, 1060
SsiI CCGC 2 cut(s) 147, 949
SspMI CTAG 5 cut(s) 90, 189, 264, 716, 869
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 5 cut(s) 843, 1078, 1099, 1177, 1243
TaiI ACGT 1 cut(s) 455
TaqI TCGA 4 cut(s) 610, 633, 809, 845
TasI AATT 6 cut(s) 157, 395, 485, 662, 769, 1060
TfiI GAWTC 5 cut(s) 254, 635, 686, 734, 896
Tru1I TTAA 2 cut(s) 675, 954
Tru9I TTAA 2 cut(s) 675, 954
TseFI GTSAC 2 cut(s) 139, 150
Tsp45I GTSAC 2 cut(s) 139, 150
TspDTI ATGAA 2 cut(s) 179, 909
XapI RAATTY 2 cut(s) 395, 769
XbaI TCTAGA 1 cut(s) 263
XhoI CTCGAG 1 cut(s) 609
XspI CTAG 5 cut(s) 90, 189, 264, 716, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.