FvH4_5g29010

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003606
Physical Location & Seq
Forward (+)
20137319 .. 20141105
3787 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29010.t2

Sequence Viewer

Length: 2709 bp
ATGGTCCAAAGGAAGGTACAAACCAAGCTTGCAATCCAAGTAGATCCTAATGTCAAGTTCGAGAAGCGGTTGGTAAACCTTAAACCATCTTCTCAGTTCCAAGATGGCAAAAATAGAGGAGGAGCTGATTTGAAGAAGAAGAAGATGAAGAAGTCAAGATCAACCAAGCTTTCAGATATTGACAGCTTGAGATCACCATCACCATCTTCATCACTTCCTTTGAGAAATAGCATGTCCCTGCCCGGAAAGCCACTGCCTCTCAATGTTCAAAGCATTTCAGCAGCAGCAGTCCCTCTGAAGCAGGCTCTCACCAAAACAACTGATGGCTCAGCACCAAACTATATGAAGTCCACCAGCTGTTCAGTTGCAAGGAAGGAGCAATCACAGGTTAGTGTTAGAAATTCTCCGGTTAATTCGGATAGTAGGAATCAAAACCGAAGAAATTTGAGCAACTCAAAACTTAGCTCTGGCTCTATCAATAAACCAGCTCGGACATCTAGTCTGAAGTTGGTGAGAACTCTAATAAAGTCTCCTAGTTTTAAGCCTGCAAGAACTTCAGCCAAGAAAAATTCCAGGGTTGCTCTTTGTGAAGATGTAAATATTCAGAAAGCAACCTGTTCTTCAACACTGAAGGACTCCAAGTTTCCCGAGTATCTGATGATTAGTCCTGGCGGAACAGAAGCTGAGGGAACTTCAGTGATGAAGGTTTGCCCCTATACTTACTGCTCTCTCAATGGACATCATCATCAACCTGTGACTCCACTGAAGTGTTTCTTGTCGGCACGAAGACGGTCTTTGAAGAACCAGAAGATGGTGAAACTGCAAGCTCTAAGTCCTCGTAAATCAAAGCCATCTAATAAGGGAATGAATGAAAATGGTTTGAAGCAGATGTGTTTTAATGATAATGAAAAACCTGCTCAACAAGAAGTGGGGATCGATTACTTTGTTGAAATATTTGCTAAAAGCAAGGACGATGATGCTCAAACAATTGTGAAGAATATACCTGAAGCAGAAATTATAGATAGCTTTCCCGGGGAGGAGCAAAAAGAAGATGTGGCAGATGAAGTATACCAACCTCTGGATCAAGAAGCAGATGAAGTATACCAACCTTCTTTGGATCAAGAACCTGCCATGCGAAGTTGTTCGAGTGAGAGTGAATCGGATGGACTTTCAAGCATTGAAGTGGATTATGCCAATTCTGAAGCAACTGAGATGGAGTGGGAGGAAGGCCAATTTTCCGTTGCAGTGCTGGATGATGAATCCGGTTCAAAAGCTGGGCTTTCATCAATTCAAGATGGTGATATGCATGAAGAACCTGTGATCAAGTTTGATGCCATTGTTAGTAACTGCAACGACATCATTCATGATTACTATCAAGTACTGCAAGAACTTTTCGAAGAGACAACTCCATCAGTTGAAATTCAATTAAACAATGATGGCACAAAACAAAATTTGGACACTGAAGATTCTGATAGAATGAGTTACGACCAATATTCTTACACTGAGGATTCATTTGAAGAGGACAATGAACTATCAGAGACAGAAATAGAGATATCTTCATCTTCAGTAGAGGAAGCAGCAACTGGTGAGGAAATTCAAGAGAAACCTGGAGTTCTCAAAGCAGAGGATCAGGAAATGGAGTCCCATCTTGGAAATGTTGAAAGCCACTGCACTGCTGCTAGTGAGACAGATGAAGCTGCAGATAGCCAACCAGGGAATGAAGATGATGGAACAACTATGTCAACAAGTAACCAAATTTCTAATGCCACTCAGGATAACAGCACAACATACGGAGCTGAAACAAATGAAGAGAAAGAGAACTCAGGAATAGATCACAATGTTGTCATGGCTGTTTCAGGAAATCAAATGGAAGAGGCACAGGAAGTTGATGATTCAAAATCCTCCGCAGATACCAAGAATCTCGATTCAGCTAAGACTATGGTTGTACACGATGAAGATGCTAATAAGGTTGGTGATAATGCAGACACTGCCAAAGATTATAACTCAAGCCAAGATATTATTGATGAAACCACTTCTGTTAAGAGTGAAGATAACTTGGCTGATGGGGAGTACACAGATAATGCTAAAGCAGAGAAACATAGCTTCACAGATGAGGATCAAAGTGAATTTAAGAAGGAAAAGATCTCAAGTTCCACTGAAGGTGAGGAACATAGTGATCTAAAGCTGAAGAAAATAGGTTTGGCAGAAAACAGTGTCAGATACATTGATAGAATGGAAGTGGACAACATTTCAAAGCCAGATGCAGCAGAAACTTTCTTCATGGCCACTACCAGTGCCAGTCCAGGGATGAAGAGGAAGCTTTCCCATATAGAAAGCAATTCTGACGAAGAACTGCTAAACACTAACCGGAAATGGAAAATCAACTGCAAGAGAACTATCAAGGATGAGGAGGAACTACAGAAATACACTCAAACAGAACCCAATTACCTGCCGTTGATTCCTGGTCCTGAAGCAGAGAAGGTTGACTTGAGGCATCAGATGTTGGATGAAAAGAAAAATGCAGAGGAATGGATGCTCGACTTTGCAATCCAGCAAGCTGTAACCAAACTCGCTCCAGCTAGGAAGAAGAAGGTGGCATTGCTTGTTGAAGCTTTTGAAAAAGTCATGCCAGCGCCAAAATATGAACCCCGTTTGAAGCATTCTTCAACAGCCTTCAGTCATGCAAGACCTATGCAAGCTTGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

903

Amino Acids

100.08

Weight (kDa)

4.86

Isoelectric Point (pI)

53.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 508 - 607 4.6e-28 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2001
Acc36I ACCTGC 3 cut(s) 922, 1135, 2457
AccB7I CCANNNNNTGG 2 cut(s) 811, 1078
AccI GTMKAC 2 cut(s) 1068, 1101
AciI CCGC 3 cut(s) 67, 672, 1905
AclWI GGATC 6 cut(s) 38, 941, 1089, 1125, 1635, 2124
AcoI YGGCCR 1 cut(s) 2283
AcsI RAATTY 8 cut(s) 400, 442, 568, 1419, 1450, 1593, 1755, 2126
AfaI GTAC 4 cut(s) 18, 1380, 1947, 2072
AfiI CCNNNNNNNGG 4 cut(s) 13, 811, 1078, 2304
AhdI GACNNNNNGTC 1 cut(s) 498
AjnI CCWGG 6 cut(s) 572, 667, 1606, 1711, 2302, 2461
AjuI GAANNNNNNNTTGG 2 cut(s) 2183, 2215
AleI CACNNNNGTG 1 cut(s) 766
Alw26I GTCTC 4 cut(s) 534, 1394, 1532, 1679
AlwI GGATC 6 cut(s) 38, 941, 1089, 1125, 1635, 2124
AlwNI CAGNNNCTG 3 cut(s) 683, 1583, 1988
Ama87I CYCGRG 2 cut(s) 647, 1031
AoxI GGCC 2 cut(s) 1228, 2283
ApeKI GCWGC 6 cut(s) 281, 284, 1577, 1676, 1697, 2264
ApoI RAATTY 8 cut(s) 400, 442, 568, 1419, 1450, 1593, 1755, 2126
Asp700I GAANNNNTTC 2 cut(s) 770, 1141
AspLEI GCGC 1 cut(s) 2635
AspS9I GGNCC 2 cut(s) 4, 2465
AsuC2I CCSGG 3 cut(s) 243, 1032, 1033
AsuHPI GGTGA 9 cut(s) 186, 192, 301, 523, 826, 1310, 1598, 1985, 2174
AsuII TTCGAA 1 cut(s) 1395
AvaI CYCGRG 2 cut(s) 647, 1031
AvaII GGWCC 2 cut(s) 4, 2465
BalI TGGCCA 1 cut(s) 2285
BbsI GAAGAC 1 cut(s) 793
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 6 cut(s) 293, 296, 1589, 1663, 1684, 2276
BceAI ACGGC 1 cut(s) 2437
BcgI CGANNNNNNTGC 2 cut(s) 1940, 1974
BciT130I CCWGG 6 cut(s) 574, 669, 1608, 1713, 2304, 2463
BclI TGATCA 1 cut(s) 1320
BcnI CCSGG 3 cut(s) 243, 1032, 1033
BcoDI GTCTC 4 cut(s) 534, 1394, 1532, 1679
BfaI CTAG 4 cut(s) 498, 534, 1680, 2580
BfmI CTRYAG 2 cut(s) 1698, 2417
BfoI RGCGCY 1 cut(s) 2636
BfuAI ACCTGC 3 cut(s) 922, 1135, 2457
BglII AGATCT 1 cut(s) 2142
BisI GCNGC 6 cut(s) 282, 285, 1578, 1677, 1698, 2265
BlpI GCTNAGC 1 cut(s) 328
BlsI GCNGC 6 cut(s) 283, 286, 1579, 1678, 1699, 2266
BmcAI AGTACT 1 cut(s) 1380
Bme1390I CCNGG 9 cut(s) 243, 574, 669, 1032, 1033, 1608, 1713, 2304, 2463
Bme18I GGWCC 2 cut(s) 4, 2465
BmeRI GACNNNNNGTC 1 cut(s) 498
BmeT110I CYCGRG 2 cut(s) 647, 1031
BmgT120I GGNCC 2 cut(s) 4, 2465
BmrFI CCNGG 9 cut(s) 243, 574, 669, 1032, 1033, 1608, 1713, 2304, 2463
BmsI GCATC 6 cut(s) 967, 1321, 1948, 2251, 2503, 2523
BpiI GAAGAC 1 cut(s) 793
BpmI CTGGAG 2 cut(s) 1629, 2559
Bpu10I CCTNAGC 1 cut(s) 684
Bpu1102I GCTNAGC 1 cut(s) 328
Bpu14I TTCGAA 1 cut(s) 1395
BpuEI CTTGAG 4 cut(s) 208, 1990, 2131, 2509
BpuMI CCSGG 3 cut(s) 243, 1032, 1033
Bsa29I ATCGAT 1 cut(s) 936
BsaBI GATNNNNATC 3 cut(s) 196, 1371, 2115
BsaJI CCNNGG 5 cut(s) 573, 1031, 1032, 1712, 2303
BsaWI WCCGGW 3 cut(s) 406, 1262, 2367
Bsc4I CCNNNNNNNGG 4 cut(s) 13, 811, 1078, 2304
Bse1I ACTGG 3 cut(s) 1588, 2292, 2298
Bse3DI GCAATG 1 cut(s) 2597
Bse8I GATNNNNATC 3 cut(s) 196, 1371, 2115
BseBI CCWGG 6 cut(s) 574, 669, 1608, 1713, 2304, 2463
BseCI ATCGAT 1 cut(s) 936
BseDI CCNNGG 5 cut(s) 573, 1031, 1032, 1712, 2303
BseGI GGATG 6 cut(s) 1168, 1258, 2313, 2410, 2512, 2538
BseJI GATNNNNATC 3 cut(s) 196, 1371, 2115
BseLI CCNNNNNNNGG 4 cut(s) 13, 811, 1078, 2304
BseMI GCAATG 1 cut(s) 2597
BseMII CTCAG 7 cut(s) 107, 342, 675, 1200, 1494, 1784, 1836
BseNI ACTGG 3 cut(s) 1588, 2292, 2298
BseRI GAGGAG 4 cut(s) 132, 135, 1052, 2423
BseXI GCAGC 6 cut(s) 293, 296, 1589, 1663, 1684, 2276
BseYI CCCAGC 1 cut(s) 1274
BsgI GTGCAG 1 cut(s) 1654
BshFI GGCC 2 cut(s) 1230, 2285
BshVI ATCGAT 1 cut(s) 936
BsiHKCI CYCGRG 2 cut(s) 647, 1031
BsiSI CCGG 5 cut(s) 243, 407, 1032, 1263, 2368
BslFI GGGAC 3 cut(s) 220, 275, 1627
BslI CCNNNNNNNGG 4 cut(s) 13, 811, 1078, 2304
BsmAI GTCTC 4 cut(s) 534, 1394, 1532, 1679
BsmFI GGGAC 3 cut(s) 220, 275, 1627
BsmI GAATGC 1 cut(s) 2659
BsnI GGCC 2 cut(s) 1230, 2285
BsoBI CYCGRG 2 cut(s) 647, 1031
Bsp119I TTCGAA 1 cut(s) 1395
Bsp1407I TGTACA 1 cut(s) 1945
Bsp1720I GCTNAGC 1 cut(s) 328
BspACI CCGC 3 cut(s) 67, 672, 1905
BspANI GGCC 2 cut(s) 1230, 2285
BspCNI CTCAG 7 cut(s) 106, 341, 676, 1201, 1495, 1783, 1835
BspDI ATCGAT 1 cut(s) 936
BspHI TCATGA 1 cut(s) 1363
BspMAI CTGCAG 1 cut(s) 1702
BspMI ACCTGC 3 cut(s) 922, 1135, 2457
BspPI GGATC 6 cut(s) 38, 941, 1089, 1125, 1635, 2124
BspT104I TTCGAA 1 cut(s) 1395
BsrDI GCAATG 1 cut(s) 2597
BsrGI TGTACA 1 cut(s) 1945
BsrI ACTGG 3 cut(s) 1588, 2292, 2298
BssECI CCNNGG 5 cut(s) 573, 1031, 1032, 1712, 2303
BssNAI GTATAC 2 cut(s) 1069, 1102
Bst1107I GTATAC 2 cut(s) 1069, 1102
Bst2UI CCWGG 6 cut(s) 574, 669, 1608, 1713, 2304, 2463
Bst4CI ACNGT 2 cut(s) 792, 2213
Bst6I CTCTTC 5 cut(s) 1392, 1512, 1803, 1866, 2306
BstAPI GCANNNNNTGC 1 cut(s) 1988
BstAUI TGTACA 1 cut(s) 1945
BstBI TTCGAA 1 cut(s) 1395
BstC8I GCNNGC 7 cut(s) 30, 303, 546, 825, 2556, 2631, 2697
BstF5I GGATG 6 cut(s) 1168, 1258, 2313, 2410, 2512, 2538
BstH2I RGCGCY 1 cut(s) 2636
BstHHI GCGC 1 cut(s) 2635
BstMAI GTCTC 4 cut(s) 534, 1394, 1532, 1679
BstMWI GCNNNNNNNGC 2 cut(s) 247, 1988
BstNI CCWGG 6 cut(s) 574, 669, 1608, 1713, 2304, 2463
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 9 cut(s) 241, 572, 667, 1030, 1031, 1606, 1711, 2302, 2461
BstSFI CTRYAG 2 cut(s) 1698, 2417
BstV1I GCAGC 6 cut(s) 293, 296, 1589, 1663, 1684, 2276
BstV2I GAAGAC 1 cut(s) 793
BstX2I RGATCY 2 cut(s) 43, 2142
BstYI RGATCY 2 cut(s) 43, 2142
BstZ17I GTATAC 2 cut(s) 1069, 1102
Bsu15I ATCGAT 1 cut(s) 936
BsuRI GGCC 2 cut(s) 1230, 2285
BsuTUI ATCGAT 1 cut(s) 936
BtsCI GGATG 6 cut(s) 1168, 1258, 2313, 2410, 2512, 2538
BtsI GCAGTG 5 cut(s) 251, 1251, 1666, 1671, 1986
BveI ACCTGC 3 cut(s) 922, 1135, 2457
Cac8I GCNNGC 7 cut(s) 30, 303, 546, 825, 2556, 2631, 2697
CaiI CAGNNNCTG 3 cut(s) 683, 1583, 1988
CciI TCATGA 1 cut(s) 1363
CfoI GCGC 1 cut(s) 2635
Cfr13I GGNCC 2 cut(s) 4, 2465
Cfr9I CCCGGG 1 cut(s) 1031
ClaI ATCGAT 1 cut(s) 936
Csp6I GTAC 4 cut(s) 17, 1379, 1946, 2071
CviAII CATG 8 cut(s) 232, 1132, 1307, 1364, 1846, 2281, 2626, 2681
CviQI GTAC 4 cut(s) 17, 1379, 1946, 2071
DriI GACNNNNNGTC 1 cut(s) 498
EaeI YGGCCR 1 cut(s) 2283
Eam1104I CTCTTC 5 cut(s) 1392, 1512, 1803, 1866, 2306
Eam1105I GACNNNNNGTC 1 cut(s) 498
EarI CTCTTC 5 cut(s) 1392, 1512, 1803, 1866, 2306
EciI GGCGGA 1 cut(s) 687
Eco32I GATATC 1 cut(s) 1554
Eco47I GGWCC 2 cut(s) 4, 2465
Eco88I CYCGRG 2 cut(s) 647, 1031
EcoRII CCWGG 6 cut(s) 572, 667, 1606, 1711, 2302, 2461
EcoRV GATATC 1 cut(s) 1554
EcoT22I ATGCAT 1 cut(s) 1308
FaeI CATG 8 cut(s) 235, 1135, 1310, 1367, 1849, 2284, 2629, 2684
FalI AAGNNNNNCTT 8 cut(s) 758, 790, 778, 810, 1263, 1295, 2471, 2503
FaqI GGGAC 3 cut(s) 220, 275, 1627
FatI CATG 8 cut(s) 231, 1131, 1306, 1363, 1845, 2280, 2625, 2680
FbaI TGATCA 1 cut(s) 1320
FblI GTMKAC 2 cut(s) 1068, 1101
Fnu4HI GCNGC 6 cut(s) 282, 285, 1578, 1677, 1698, 2265
FokI GGATG 6 cut(s) 1175, 1265, 2320, 2417, 2519, 2545
Fsp4HI GCNGC 6 cut(s) 282, 285, 1578, 1677, 1698, 2265
FspBI CTAG 4 cut(s) 498, 534, 1680, 2580
GlaI GCGC 1 cut(s) 2634
GluI GCNGC 6 cut(s) 282, 285, 1578, 1677, 1698, 2265
GsaI CCCAGC 1 cut(s) 1278
GsuI CTGGAG 2 cut(s) 1629, 2559
HaeII RGCGCY 1 cut(s) 2636
HaeIII GGCC 2 cut(s) 1230, 2285
HapII CCGG 5 cut(s) 243, 407, 1032, 1263, 2368
HhaI GCGC 1 cut(s) 2635
Hin1II CATG 8 cut(s) 235, 1135, 1310, 1367, 1849, 2284, 2629, 2684
Hin6I GCGC 1 cut(s) 2633
HinP1I GCGC 1 cut(s) 2633
HincII GTYRAC 2 cut(s) 1743, 2485
HindII GTYRAC 2 cut(s) 1743, 2485
HindIII AAGCTT 5 cut(s) 26, 167, 2318, 2610, 2697
HpaII CCGG 5 cut(s) 243, 407, 1032, 1263, 2368
HphI GGTGA 9 cut(s) 186, 192, 301, 523, 826, 1310, 1598, 1985, 2174
Hpy166II GTNNAC 9 cut(s) 76, 351, 1069, 1102, 1743, 1948, 2073, 2242, 2485
Hpy8I GTNNAC 9 cut(s) 76, 351, 1069, 1102, 1743, 1948, 2073, 2242, 2485
HpyCH4III ACNGT 2 cut(s) 792, 2213
HpyF10VI GCNNNNNNNGC 2 cut(s) 247, 1988
Hsp92II CATG 8 cut(s) 235, 1135, 1310, 1367, 1849, 2284, 2629, 2684
HspAI GCGC 1 cut(s) 2633
Ksp22I TGATCA 1 cut(s) 1320
LmnI GCTCC 5 cut(s) 122, 376, 1039, 1793, 2578
Lsp1109I GCAGC 6 cut(s) 293, 296, 1589, 1663, 1684, 2276
LweI GCATC 6 cut(s) 967, 1321, 1948, 2251, 2503, 2523
MaeI CTAG 4 cut(s) 498, 534, 1680, 2580
MaeIII GTNAC 5 cut(s) 754, 1343, 1481, 1748, 2560
MfeI CAATTG 1 cut(s) 987
MflI RGATCY 2 cut(s) 43, 2142
MlsI TGGCCA 1 cut(s) 2285
MluNI TGGCCA 1 cut(s) 2285
MlyI GAGTC 3 cut(s) 629, 751, 1649
MmeI TCCRAC 1 cut(s) 2484
Mox20I TGGCCA 1 cut(s) 2285
Mph1103I ATGCAT 1 cut(s) 1308
MroXI GAANNNNTTC 2 cut(s) 770, 1141
MscI TGGCCA 1 cut(s) 2285
MseI TTAA 7 cut(s) 81, 411, 540, 897, 1427, 2040, 2130
MslI CAYNNNNRTG 2 cut(s) 766, 1181
Msp20I TGGCCA 1 cut(s) 2285
MspA1I CMGCKG 1 cut(s) 357
MspI CCGG 5 cut(s) 243, 407, 1032, 1263, 2368
MspR9I CCNGG 9 cut(s) 243, 574, 669, 1032, 1033, 1608, 1713, 2304, 2463
MunI CAATTG 1 cut(s) 987
Mva1269I GAATGC 1 cut(s) 2659
MvaI CCWGG 6 cut(s) 574, 669, 1608, 1713, 2304, 2463
MwoI GCNNNNNNNGC 2 cut(s) 247, 1988
NciI CCSGG 3 cut(s) 243, 1032, 1033
NlaIII CATG 8 cut(s) 235, 1135, 1310, 1367, 1849, 2284, 2629, 2684
NmuCI GTSAC 1 cut(s) 754
NsiI ATGCAT 1 cut(s) 1308
NspI RCATGY 1 cut(s) 235
NspV TTCGAA 1 cut(s) 1395
OliI CACNNNNGTG 1 cut(s) 766
PagI TCATGA 1 cut(s) 1363
PctI GAATGC 1 cut(s) 2659
PdmI GAANNNNTTC 2 cut(s) 770, 1141
PfeI GAWTC 9 cut(s) 427, 1157, 1259, 1466, 1508, 1892, 1918, 1925, 2458
PflMI CCANNNNNTGG 2 cut(s) 811, 1078
PkrI GCNGC 6 cut(s) 283, 286, 1579, 1678, 1699, 2266
PleI GAGTC 3 cut(s) 629, 751, 1648
PpsI GAGTC 3 cut(s) 629, 751, 1648
PsiI TTATAA 1 cut(s) 2001
Psp6I CCWGG 6 cut(s) 572, 667, 1606, 1711, 2302, 2461
PspFI CCCAGC 1 cut(s) 1274
PspGI CCWGG 6 cut(s) 572, 667, 1606, 1711, 2302, 2461
PspPI GGNCC 2 cut(s) 4, 2465
PstI CTGCAG 1 cut(s) 1702
PstNI CAGNNNCTG 3 cut(s) 683, 1583, 1988
PsuI RGATCY 2 cut(s) 43, 2142
PvuII CAGCTG 1 cut(s) 357
RsaI GTAC 4 cut(s) 18, 1380, 1947, 2072
RsaNI GTAC 4 cut(s) 17, 1379, 1946, 2071
RseI CAYNNNNRTG 2 cut(s) 766, 1181
SaqAI TTAA 7 cut(s) 81, 411, 540, 897, 1427, 2040, 2130
SatI GCNGC 6 cut(s) 282, 285, 1578, 1677, 1698, 2265
Sau96I GGNCC 2 cut(s) 4, 2465
ScaI AGTACT 1 cut(s) 1380
SchI GAGTC 3 cut(s) 629, 751, 1649
ScrFI CCNGG 9 cut(s) 243, 574, 669, 1032, 1033, 1608, 1713, 2304, 2463
SfaNI GCATC 6 cut(s) 967, 1321, 1948, 2251, 2503, 2523
SfcI CTRYAG 2 cut(s) 1698, 2417
SfuI TTCGAA 1 cut(s) 1395
SinI GGWCC 2 cut(s) 4, 2465
SmaI CCCGGG 1 cut(s) 1033
SmiMI CAYNNNNRTG 2 cut(s) 766, 1181
SmlI CTYRAG 4 cut(s) 187, 2005, 2146, 2488
SmoI CTYRAG 4 cut(s) 187, 2005, 2146, 2488
SsiI CCGC 3 cut(s) 67, 672, 1905
SspI AATATT 3 cut(s) 601, 954, 1493
SspMI CTAG 4 cut(s) 498, 534, 1680, 2580
StyD4I CCNGG 9 cut(s) 241, 572, 667, 1030, 1031, 1606, 1711, 2302, 2461
TaaI ACNGT 2 cut(s) 792, 2213
TaqI TCGA 6 cut(s) 60, 936, 1145, 1395, 1923, 2538
TatI WGTACW 3 cut(s) 1378, 1945, 2070
TfiI GAWTC 9 cut(s) 427, 1157, 1259, 1466, 1508, 1892, 1918, 1925, 2458
Tru1I TTAA 7 cut(s) 81, 411, 540, 897, 1427, 2040, 2130
Tru9I TTAA 7 cut(s) 81, 411, 540, 897, 1427, 2040, 2130
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 6 cut(s) 281, 284, 1577, 1676, 1697, 2264
Tsp45I GTSAC 1 cut(s) 754
TspGWI ACGGA 2 cut(s) 1228, 1806
TspMI CCCGGG 1 cut(s) 1031
Van91I CCANNNNNTGG 2 cut(s) 811, 1078
VpaK11BI GGWCC 2 cut(s) 4, 2465
XapI RAATTY 8 cut(s) 400, 442, 568, 1419, 1450, 1593, 1755, 2126
XceI RCATGY 1 cut(s) 235
XmaI CCCGGG 1 cut(s) 1031
XmiI GTMKAC 2 cut(s) 1068, 1101
XmnI GAANNNNTTC 2 cut(s) 770, 1141
XspI CTAG 4 cut(s) 498, 534, 1680, 2580
ZrmI AGTACT 1 cut(s) 1380
Zsp2I ATGCAT 1 cut(s) 1308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.