MD07G1032000.v1.1

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003606
Physical Location & Seq
Reverse (-)
2598042 .. 2601367
3326 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1032000.v1.1.491

Sequence Viewer

Length: 2820 bp
ATGGTCCAAAGAAAGGTGCCAAACAAGCTTGGAATCCAAGCTGATCATGACAAATTCGAGAAGCGGTTTTCAAGCCTGAAGACTTCTTCTCAGTTCCAAGATGGGAAACACAGAGGAGCTGATTTGAAGAAGAAGAAGAAGATGAAGAAATCTAGATCAATCAAGCTTTCAGATGTTGAGAGCTTGAGATCATCAACTTTGAGGAAGAACTTATCTCAGCCAGGAAAGCCGCCACCGCTGCCTCTGAATGTTCCTAACACTGCGGCTTCCCCTCAGAAGCAGCCTCTGACCAAGACAACTTATGGCTCGCCAAACTACATGAAGCCCACCAGCTGTTCTCATGCAAGGAAGGAGCAGTCACAGATTAGTCTTCGAAGTTCTCCGCCTATATTTTCGGATAGTAAGAACCAGAACCGAAAAAATTCAGGCAGTTCAAAGCTTAGCTCTGCTTCTAGTAAACCTGAAAGAAGTTTAGCAAGGACATCTGGTTTGAAGCTTGTGAGAACTTTAATGAAGTCCCCTAGTTTTAAGCCGGTAAGAGCTTCGGCGAAAAAAACTTCCAGGGTTGTTCTTTGTGCAGATGCGAATGTTCAGAGAGCAACTTGTTCTTCAACACTGAAGGATGCAAAGTTTCCTGACTATCTGACGATTAGTCCTGGGGGAACCGAAGCTGAGGGAACTTCAATCATGAAGGTTTGCCCGTATACGTACTGTTCTCTTAATGGCCATCATCGTTCGCCTGTGCCCCCATTGAAGAGTTTCTTGTCTGCAAGAAGACGTTCGTTGAAGACCCAGAAGATGATGAAATTGCAAGCTCTGAGTCCACGCAGAGCAAAGCGATGTAATGATGGAATAAAAGAAATTCATTTGCAGAAAATGTTGTTTGATGAGGATGAAAAAATTGCAGAGAAAGAAGCTGCTTTGGATTTCTTTGTTGAATTCTATGCTACAAACGAGGAAGATGGCACCGAGGCAATTGGAAGGAAAGCAGGAGCTGATTTTGTAGGAGAACAAGAAGTTTACGAGGGTCCTGCGTTTCCAAATGATGCAAGTGATGAAGCAGAAACAGAAAATTATGCCAACAACCTAGTTGTGGAGAACCTGTCAGATAGATCACCGCATTCGGAGAGTGAATCTGAGGCAGAAAGCTTCGAAGAATTTCCTGAAGAAGACCAAAAAGAAGATGCAGATGAAGACTACGGACCACAGTCAGATCAAGAAGACAATTCCACTGGAAGCTGCTTGAAAGAGAGCGACTCTGAAGACCTTTCAAGCACTGAAATTGATTATTCCAGTTCTGAAAGTATTGACATGGAGTGGGAGGAAGGGCAATTTGCCACTACAGTGCTTGGTGATTATGAATCTGTTCCAAATGCCGGGTGTTCAACAAAAATTCAAGACACTGATAAGCAAGAAGAATCGCTGATCAACTCTGACGCAATGAAAGGTAACAGCGATAATATGATTCAGGATTATTATGGAGTACTGCAGAATGATGGTGATGTCAGTGAACAGGATGTCATTAAACAGAATTTTGAGATTCAAGAGTCTGAGCAAGAGTATGAAAGATGGAGTTACGACCAACTTTCTTACACTGAGGATGCATTTGAAGGAGGCAGTGAACTATCGGATACAGATTGCATAGAAATATCTTCAACAGAGGAGCCAACTGAGGAACTAACAGGAACTGGTGAGGAAATTCAAGAACAAAATGAATGTTTTGAAGCAGAGGATCATGAGATCGGCTCTCACCTTGGAGATGTTGAAAGCAACTACACCAGTGTGGAGACAGATGAAGCTTCAGATAACCAACCAAAGAATACATTTCATGAAGATGAAACAAGTACGCTGACAGGAGACCGAATTTCTATTCCTTCTCAGGATATCTGCGACACAGATGATGCTGAAACAGATGCAGGATGCAACGGGAGACCTGATGAAGAGAATTCTGAAACAAATCAAAATGTTGCAACTGGTGACTTTGGCTTGGAACCTGGACTCCCTGCAGGGGTTCCTGAAAATCAAATGGAAGCAACAGAGCAAATTGGTGATGTCAAACCATCACCGGAGATTCAGCTTTCTGATTCGGTGCATGATGCTTCTGAAGCTGATGAAGATGCTGTCAAGGTTGACGATTATGATGATGGCAAGAAGACTGAACCTTTCCAGCATTATGACATTGCTGAAGATGGTAACCTGTCCAAAGGGAAGTACACAAAGCCCAAAATTTCAAGTTCCAATGAATCTGAGGATCAAAGTGATTTAAGGCTGAAAAAATCAGATATCTTTGGTAACAACACAGGAGAACCTGATAACATGGAAGTGGAAAACAATTCAGAGCCAGAAGCCACAAAAAATTTCAACACGGCCAATAATAGCATTAGCCCTGGGATGAAGAGAAAATTTTCTGAAGCAGCAAGCAACTTCGACCAAGAACTTCCCAACACCTACAACTACTGGAAAAGAATCAAATGCAAGAAACTCAACATGGATGGGGAGGAGCAAAGGAAGTTCAATCCAAGAGAACCGAATTACCTGCCTTTGGTTCCTGACCCCGAACCAGAAAAGGTTGACCTCAGGCATCAGATTATCGACAACAAGAAAAATACAGATGAATGGATGCTTGACTATGCACTCCAGCAAGCTGTCACAAAACTTGCTCCTGCAAGGAAGAAGAAGGTGGCACTGCTTGTTGCAGCTTTCGAAACGGTCATGCCAGCGCCCAAAGTGGGAAAACATCATCGGCATACATCAACAGGCTTCAGTCACGCAAGACCTATGCAAGCTTGTGAAAAGACTGCAACATCTATAAGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

940

Amino Acids

104.57

Weight (kDa)

4.75

Isoelectric Point (pI)

56.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 810 - 906 4.9e-30 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2544
AccB1I GGYRCC 2 cut(s) 16, 965
AccI GTMKAC 1 cut(s) 704
AciI CCGC 6 cut(s) 64, 230, 236, 263, 383, 1118
AclWI GGATC 2 cut(s) 1740, 2259
AcoI YGGCCR 2 cut(s) 724, 2367
AcuI CTGAAG 9 cut(s) 98, 638, 1185, 1281, 1785, 2124, 2205, 2430, 2746
AfaI GTAC 4 cut(s) 710, 1485, 1846, 2213
AfiI CCNNNNNNNGG 6 cut(s) 13, 1093, 1376, 2008, 2542, 2729
AhdI GACNNNNNGTC 1 cut(s) 651
AjnI CCWGG 5 cut(s) 220, 560, 655, 1993, 2386
AleI CACNNNNGTG 2 cut(s) 1343, 1781
AloI GAACNNNNNNTCC 2 cut(s) 1983, 2015
Alw26I GTCTC 3 cut(s) 1781, 1851, 1924
AlwI GGATC 2 cut(s) 1740, 2259
AlwNI CAGNNNCTG 3 cut(s) 286, 995, 1688
AoxI GGCC 2 cut(s) 724, 2367
ApeKI GCWGC 6 cut(s) 238, 280, 917, 1239, 2414, 2696
ArsI GACNNNNNNTTYG 2 cut(s) 472, 504
Asp700I GAANNNNTTC 6 cut(s) 421, 758, 778, 1158, 1365, 2404
AspLEI GCGC 1 cut(s) 2722
AspS9I GGNCC 3 cut(s) 4, 1028, 1202
AsuC2I CCSGG 1 cut(s) 1378
AsuHPI GGTGA 8 cut(s) 1107, 1364, 1511, 1703, 1742, 1988, 2055, 2060
AsuII TTCGAA 3 cut(s) 373, 1152, 2703
AvaII GGWCC 3 cut(s) 4, 1028, 1202
AxyI CCTNAGG 1 cut(s) 2576
BaeGI GKGCMC 1 cut(s) 747
BalI TGGCCA 1 cut(s) 726
BanI GGYRCC 2 cut(s) 16, 965
BbsI GAAGAC 9 cut(s) 86, 362, 781, 794, 1176, 1200, 1227, 1269, 2159
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 6 cut(s) 225, 292, 904, 1226, 2426, 2708
BceAI ACGGC 1 cut(s) 2382
BcgI CGANNNNNNTGC 4 cut(s) 1013, 1047, 2518, 2552
BciT130I CCWGG 5 cut(s) 222, 562, 657, 1995, 2388
BciVI GTATCC 1 cut(s) 1624
BclI TGATCA 2 cut(s) 43, 1425
BcnI CCSGG 1 cut(s) 1378
BcoDI GTCTC 3 cut(s) 1781, 1851, 1924
BfaI CTAG 4 cut(s) 153, 453, 522, 1088
BfmI CTRYAG 3 cut(s) 1341, 1487, 2004
BfoI RGCGCY 1 cut(s) 2723
BfuAI ACCTGC 1 cut(s) 2544
BfuI GTATCC 1 cut(s) 1624
BisI GCNGC 8 cut(s) 230, 239, 264, 281, 918, 1240, 2415, 2697
BlpI GCTNAGC 1 cut(s) 440
BlsI GCNGC 8 cut(s) 231, 240, 265, 282, 919, 1241, 2416, 2698
BmcAI AGTACT 1 cut(s) 1485
Bme1390I CCNGG 6 cut(s) 222, 562, 657, 1378, 1995, 2388
Bme18I GGWCC 3 cut(s) 4, 1028, 1202
BmeRI GACNNNNNGTC 1 cut(s) 651
BmgT120I GGNCC 3 cut(s) 4, 1028, 1202
BmiI GGNNCC 8 cut(s) 18, 664, 967, 1029, 1665, 1992, 2013, 2547
BmrFI CCNGG 6 cut(s) 222, 562, 657, 1378, 1995, 2388
BoxI GACNNNNGTC 1 cut(s) 1207
BpiI GAAGAC 9 cut(s) 86, 362, 781, 794, 1176, 1200, 1227, 1269, 2159
BplI GAGNNNNNCTC 4 cut(s) 1241, 1273, 1730, 1762
BpmI CTGGAG 1 cut(s) 2621
Bpu10I CCTNAGC 1 cut(s) 672
Bpu1102I GCTNAGC 1 cut(s) 440
Bpu14I TTCGAA 3 cut(s) 373, 1152, 2703
BpuEI CTTGAG 1 cut(s) 205
BpuMI CCSGG 1 cut(s) 1378
BsaAI YACGTR 1 cut(s) 708
BsaI GGTCTC 2 cut(s) 1851, 1924
BsaJI CCNNGG 6 cut(s) 561, 656, 969, 1753, 2386, 2387
BsaWI WCCGGW 1 cut(s) 2065
BsaXI ACNNNNNCTCC 4 cut(s) 1088, 1118, 1983, 2013
Bsc4I CCNNNNNNNGG 6 cut(s) 13, 1093, 1376, 2008, 2542, 2729
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 6 cut(s) 1237, 1293, 1693, 1779, 1978, 2462
Bse21I CCTNAGG 1 cut(s) 2576
Bse3DI GCAATG 2 cut(s) 1446, 2178
BseBI CCWGG 5 cut(s) 222, 562, 657, 1995, 2388
BseDI CCNNGG 6 cut(s) 561, 656, 969, 1753, 2386, 2387
BseGI GGATG 8 cut(s) 628, 898, 1522, 1606, 1925, 2397, 2497, 2625
BseLI CCNNNNNNNGG 6 cut(s) 13, 1093, 1376, 2008, 2542, 2729
BseMI GCAATG 2 cut(s) 1446, 2178
BseNI ACTGG 6 cut(s) 1237, 1293, 1693, 1779, 1978, 2462
BseRI GAGGAG 3 cut(s) 129, 1676, 2513
BseSI GKGCMC 1 cut(s) 747
BseXI GCAGC 6 cut(s) 225, 292, 904, 1226, 2426, 2708
BsgI GTGCAG 1 cut(s) 597
BshFI GGCC 2 cut(s) 726, 2369
BshNI GGYRCC 2 cut(s) 16, 965
BsiSI CCGG 3 cut(s) 533, 1377, 2066
BslFI GGGAC 1 cut(s) 502
BslI CCNNNNNNNGG 6 cut(s) 13, 1093, 1376, 2008, 2542, 2729
BsmAI GTCTC 3 cut(s) 1781, 1851, 1924
BsmFI GGGAC 1 cut(s) 502
BsmI GAATGC 1 cut(s) 1120
BsnI GGCC 2 cut(s) 726, 2369
Bso31I GGTCTC 2 cut(s) 1851, 1924
Bsp119I TTCGAA 3 cut(s) 373, 1152, 2703
Bsp1286I GDGCHC 1 cut(s) 747
Bsp143I GATC 9 cut(s) 43, 155, 188, 1112, 1213, 1425, 1732, 1740, 2251
Bsp1720I GCTNAGC 1 cut(s) 440
BspACI CCGC 6 cut(s) 64, 230, 236, 263, 383, 1118
BspANI GGCC 2 cut(s) 726, 2369
BspHI TCATGA 4 cut(s) 46, 687, 1735, 1828
BspLI GGNNCC 8 cut(s) 18, 664, 967, 1029, 1665, 1992, 2013, 2547
BspMAI CTGCAG 2 cut(s) 1491, 2008
BspMI ACCTGC 1 cut(s) 2544
BspPI GGATC 2 cut(s) 1740, 2259
BspT104I TTCGAA 3 cut(s) 373, 1152, 2703
BspT107I GGYRCC 2 cut(s) 16, 965
BspTNI GGTCTC 2 cut(s) 1851, 1924
BsrDI GCAATG 2 cut(s) 1446, 2178
BsrFI RCCGGY 1 cut(s) 532
BsrI ACTGG 6 cut(s) 1237, 1293, 1693, 1779, 1978, 2462
BssAI RCCGGY 1 cut(s) 532
BssECI CCNNGG 6 cut(s) 561, 656, 969, 1753, 2386, 2387
BssMI GATC 9 cut(s) 43, 155, 188, 1112, 1213, 1425, 1732, 1740, 2251
BssNAI GTATAC 1 cut(s) 705
BssT1I CCWWGG 1 cut(s) 1753
Bst1107I GTATAC 1 cut(s) 705
Bst2UI CCWGG 5 cut(s) 222, 562, 657, 1995, 2388
Bst4CI ACNGT 4 cut(s) 713, 1209, 1345, 2710
Bst6I CTCTTC 3 cut(s) 749, 1935, 2390
BstBAI YACGTR 1 cut(s) 708
BstBI TTCGAA 3 cut(s) 373, 1152, 2703
BstC8I GCNNGC 6 cut(s) 308, 813, 2419, 2643, 2718, 2784
BstEII GGTNACC 1 cut(s) 2192
BstF5I GGATG 8 cut(s) 628, 898, 1522, 1606, 1925, 2397, 2497, 2625
BstH2I RGCGCY 1 cut(s) 2723
BstHHI GCGC 1 cut(s) 2722
BstKTI GATC 9 cut(s) 46, 158, 191, 1115, 1216, 1428, 1735, 1743, 2254
BstMAI GTCTC 3 cut(s) 1781, 1851, 1924
BstMBI GATC 9 cut(s) 43, 155, 188, 1112, 1213, 1425, 1732, 1740, 2251
BstMWI GCNNNNNNNGC 5 cut(s) 25, 226, 235, 238, 2105
BstNI CCWGG 5 cut(s) 222, 562, 657, 1995, 2388
BstPAI GACNNNNGTC 1 cut(s) 1207
BstPI GGTNACC 1 cut(s) 2192
BstSCI CCNGG 6 cut(s) 220, 560, 655, 1376, 1993, 2386
BstSFI CTRYAG 3 cut(s) 1341, 1487, 2004
BstSLI GKGCMC 1 cut(s) 747
BstSNI TACGTA 1 cut(s) 708
BstV1I GCAGC 6 cut(s) 225, 292, 904, 1226, 2426, 2708
BstV2I GAAGAC 9 cut(s) 86, 362, 781, 794, 1176, 1200, 1227, 1269, 2159
BstZ17I GTATAC 1 cut(s) 705
Bsu36I CCTNAGG 1 cut(s) 2576
BsuI GTATCC 1 cut(s) 1624
BsuRI GGCC 2 cut(s) 726, 2369
BtgZI GCGATG 1 cut(s) 853
BtsCI GGATG 8 cut(s) 628, 898, 1522, 1606, 1925, 2397, 2497, 2625
BtsI GCAGTG 3 cut(s) 258, 1624, 2684
BveI ACCTGC 1 cut(s) 2544
Cac8I GCNNGC 6 cut(s) 308, 813, 2419, 2643, 2718, 2784
CaiI CAGNNNCTG 3 cut(s) 286, 995, 1688
CciI TCATGA 4 cut(s) 46, 687, 1735, 1828
CfoI GCGC 1 cut(s) 2722
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 3 cut(s) 4, 1028, 1202
CseI GACGC 1 cut(s) 1445
Csp6I GTAC 4 cut(s) 709, 1484, 1845, 2212
CviQI GTAC 4 cut(s) 709, 1484, 1845, 2212
DpnI GATC 9 cut(s) 45, 157, 190, 1114, 1215, 1427, 1734, 1742, 2253
DpnII GATC 9 cut(s) 43, 155, 188, 1112, 1213, 1425, 1732, 1740, 2251
DriI GACNNNNNGTC 1 cut(s) 651
EaeI YGGCCR 2 cut(s) 724, 2367
Eam1104I CTCTTC 3 cut(s) 749, 1935, 2390
Eam1105I GACNNNNNGTC 1 cut(s) 651
EarI CTCTTC 3 cut(s) 749, 1935, 2390
EciI GGCGGA 1 cut(s) 372
Eco105I TACGTA 1 cut(s) 708
Eco130I CCWWGG 1 cut(s) 1753
Eco31I GGTCTC 2 cut(s) 1851, 1924
Eco32I GATATC 2 cut(s) 1885, 2284
Eco47I GGWCC 3 cut(s) 4, 1028, 1202
Eco57I CTGAAG 9 cut(s) 98, 638, 1185, 1281, 1785, 2124, 2205, 2430, 2746
Eco81I CCTNAGG 1 cut(s) 2576
Eco91I GGTNACC 1 cut(s) 2192
EcoO109I RGGNCCY 1 cut(s) 1028
EcoO65I GGTNACC 1 cut(s) 2192
EcoRI GAATTC 2 cut(s) 938, 1945
EcoRII CCWGG 5 cut(s) 220, 560, 655, 1993, 2386
EcoRV GATATC 2 cut(s) 1885, 2284
EcoT14I CCWWGG 1 cut(s) 1753
EcoT22I ATGCAT 1 cut(s) 1606
ErhI CCWWGG 1 cut(s) 1753
FalI AAGNNNNNCTT 2 cut(s) 746, 778
FaqI GGGAC 1 cut(s) 502
FbaI TGATCA 2 cut(s) 43, 1425
FblI GTMKAC 1 cut(s) 704
Fnu4HI GCNGC 8 cut(s) 230, 239, 264, 281, 918, 1240, 2415, 2697
FokI GGATG 8 cut(s) 635, 905, 1529, 1613, 1932, 2404, 2504, 2632
Fsp4HI GCNGC 8 cut(s) 230, 239, 264, 281, 918, 1240, 2415, 2697
FspBI CTAG 4 cut(s) 153, 453, 522, 1088
GlaI GCGC 1 cut(s) 2721
GluI GCNGC 8 cut(s) 230, 239, 264, 281, 918, 1240, 2415, 2697
GsuI CTGGAG 1 cut(s) 2621
HaeII RGCGCY 1 cut(s) 2723
HaeIII GGCC 2 cut(s) 726, 2369
HapII CCGG 3 cut(s) 533, 1377, 2066
HgaI GACGC 1 cut(s) 1445
HhaI GCGC 1 cut(s) 2722
Hin6I GCGC 1 cut(s) 2720
HinP1I GCGC 1 cut(s) 2720
HincII GTYRAC 2 cut(s) 2131, 2572
HindII GTYRAC 2 cut(s) 2131, 2572
HindIII AAGCTT 7 cut(s) 26, 164, 437, 494, 1147, 1797, 2784
HpaII CCGG 3 cut(s) 533, 1377, 2066
HphI GGTGA 8 cut(s) 1107, 1364, 1511, 1703, 1742, 1988, 2055, 2060
Hpy166II GTNNAC 9 cut(s) 458, 705, 824, 1021, 1511, 1622, 2131, 2214, 2572
Hpy8I GTNNAC 9 cut(s) 458, 705, 824, 1021, 1511, 1622, 2131, 2214, 2572
HpyAV CCTTC 8 cut(s) 343, 613, 685, 975, 1319, 1604, 1884, 2671
HpyCH4III ACNGT 4 cut(s) 713, 1209, 1345, 2710
HpyCH4IV ACGT 2 cut(s) 707, 778
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 226, 235, 238, 2105
HpySE526I ACGT 2 cut(s) 707, 778
HspAI GCGC 1 cut(s) 2720
Ksp22I TGATCA 2 cut(s) 43, 1425
Kzo9I GATC 9 cut(s) 43, 155, 188, 1112, 1213, 1425, 1732, 1740, 2251
LmnI GCTCC 6 cut(s) 116, 352, 992, 1663, 2500, 2665
Lsp1109I GCAGC 6 cut(s) 225, 292, 904, 1226, 2426, 2708
MaeI CTAG 4 cut(s) 153, 453, 522, 1088
MaeII ACGT 2 cut(s) 707, 778
MaeIII GTNAC 8 cut(s) 357, 1448, 1574, 1976, 2192, 2291, 2647, 2765
MalI GATC 9 cut(s) 45, 157, 190, 1114, 1215, 1427, 1734, 1742, 2253
MboI GATC 9 cut(s) 43, 155, 188, 1112, 1213, 1425, 1732, 1740, 2251
MfeI CAATTG 1 cut(s) 975
MhlI GDGCHC 1 cut(s) 747
MlsI TGGCCA 1 cut(s) 726
MluNI TGGCCA 1 cut(s) 726
MlyI GAGTC 4 cut(s) 829, 1250, 1556, 1992
Mox20I TGGCCA 1 cut(s) 726
Mph1103I ATGCAT 1 cut(s) 1606
MroXI GAANNNNTTC 6 cut(s) 421, 758, 778, 1158, 1365, 2404
MscI TGGCCA 1 cut(s) 726
MseI TTAA 5 cut(s) 509, 528, 720, 1524, 2264
MslI CAYNNNNRTG 4 cut(s) 1343, 1781, 1833, 2321
Msp20I TGGCCA 1 cut(s) 726
MspA1I CMGCKG 2 cut(s) 238, 333
MspI CCGG 3 cut(s) 533, 1377, 2066
MspR9I CCNGG 6 cut(s) 222, 562, 657, 1378, 1995, 2388
MunI CAATTG 1 cut(s) 975
Mva1269I GAATGC 1 cut(s) 1120
MvaI CCWGG 5 cut(s) 222, 562, 657, 1995, 2388
MwoI GCNNNNNNNGC 5 cut(s) 25, 226, 235, 238, 2105
NciI CCSGG 1 cut(s) 1378
NdeII GATC 9 cut(s) 43, 155, 188, 1112, 1213, 1425, 1732, 1740, 2251
NlaIV GGNNCC 8 cut(s) 18, 664, 967, 1029, 1665, 1992, 2013, 2547
NmuCI GTSAC 4 cut(s) 357, 1976, 2647, 2765
NsiI ATGCAT 1 cut(s) 1606
NspV TTCGAA 3 cut(s) 373, 1152, 2703
OliI CACNNNNGTG 2 cut(s) 1343, 1781
PagI TCATGA 4 cut(s) 46, 687, 1735, 1828
PasI CCCWGGG 1 cut(s) 2387
PctI GAATGC 1 cut(s) 1120
PdmI GAANNNNTTC 6 cut(s) 421, 758, 778, 1158, 1365, 2404
PkrI GCNGC 8 cut(s) 231, 240, 265, 282, 919, 1241, 2416, 2698
PleI GAGTC 4 cut(s) 828, 1250, 1555, 1992
PpsI GAGTC 4 cut(s) 828, 1250, 1555, 1992
Ppu21I YACGTR 1 cut(s) 708
PpuMI RGGWCCY 1 cut(s) 1028
PshAI GACNNNNGTC 1 cut(s) 1207
Psp5II RGGWCCY 1 cut(s) 1028
Psp6I CCWGG 5 cut(s) 220, 560, 655, 1993, 2386
PspEI GGTNACC 1 cut(s) 2192
PspGI CCWGG 5 cut(s) 220, 560, 655, 1993, 2386
PspN4I GGNNCC 8 cut(s) 18, 664, 967, 1029, 1665, 1992, 2013, 2547
PspPI GGNCC 3 cut(s) 4, 1028, 1202
PspPPI RGGWCCY 1 cut(s) 1028
PstI CTGCAG 2 cut(s) 1491, 2008
PstNI CAGNNNCTG 3 cut(s) 286, 995, 1688
PvuII CAGCTG 1 cut(s) 333
RsaI GTAC 4 cut(s) 710, 1485, 1846, 2213
RsaNI GTAC 4 cut(s) 709, 1484, 1845, 2212
RseI CAYNNNNRTG 4 cut(s) 1343, 1781, 1833, 2321
SaqAI TTAA 5 cut(s) 509, 528, 720, 1524, 2264
SatI GCNGC 8 cut(s) 230, 239, 264, 281, 918, 1240, 2415, 2697
Sau3AI GATC 9 cut(s) 43, 155, 188, 1112, 1213, 1425, 1732, 1740, 2251
Sau96I GGNCC 3 cut(s) 4, 1028, 1202
SbfI CCTGCAGG 1 cut(s) 2008
ScaI AGTACT 1 cut(s) 1485
SchI GAGTC 4 cut(s) 829, 1250, 1556, 1992
ScrFI CCNGG 6 cut(s) 222, 562, 657, 1378, 1995, 2388
SdaI CCTGCAGG 1 cut(s) 2008
SduI GDGCHC 1 cut(s) 747
SfcI CTRYAG 3 cut(s) 1341, 1487, 2004
SfuI TTCGAA 3 cut(s) 373, 1152, 2703
SinI GGWCC 3 cut(s) 4, 1028, 1202
SmiMI CAYNNNNRTG 4 cut(s) 1343, 1781, 1833, 2321
SmlI CTYRAG 1 cut(s) 184
SmoI CTYRAG 1 cut(s) 184
SnaBI TACGTA 1 cut(s) 708
Sse8387I CCTGCAGG 1 cut(s) 2008
SsiI CCGC 6 cut(s) 64, 230, 236, 263, 383, 1118
SspMI CTAG 4 cut(s) 153, 453, 522, 1088
StyD4I CCNGG 6 cut(s) 220, 560, 655, 1376, 1993, 2386
StyI CCWWGG 1 cut(s) 1753
TaaI ACNGT 4 cut(s) 713, 1209, 1345, 2710
TaiI ACGT 2 cut(s) 710, 781
TaqI TCGA 6 cut(s) 57, 373, 1152, 2427, 2592, 2703
TaqII GACCGA 1 cut(s) 1875
TatI WGTACW 2 cut(s) 1483, 2211
TauI GCSGC 2 cut(s) 232, 266
Tru1I TTAA 5 cut(s) 509, 528, 720, 1524, 2264
Tru9I TTAA 5 cut(s) 509, 528, 720, 1524, 2264
TseFI GTSAC 4 cut(s) 357, 1976, 2647, 2765
TseI GCWGC 6 cut(s) 238, 280, 917, 1239, 2414, 2696
Tsp45I GTSAC 4 cut(s) 357, 1976, 2647, 2765
TspGWI ACGGA 1 cut(s) 1215
VpaK11BI GGWCC 3 cut(s) 4, 1028, 1202
XbaI TCTAGA 1 cut(s) 152
XmiI GTMKAC 1 cut(s) 704
XmnI GAANNNNTTC 6 cut(s) 421, 758, 778, 1158, 1365, 2404
XspI CTAG 4 cut(s) 153, 453, 522, 1088
ZrmI AGTACT 1 cut(s) 1485
Zsp2I ATGCAT 1 cut(s) 1606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.