Rw1G003450

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003606
Physical Location & Seq
Reverse (-)
6438093 .. 6441591
3499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G003450.1

Sequence Viewer

Length: 2640 bp
ATGGTCCAAAGAAAGGTACAAAACCAGCTTGCAAAACAAGCAGATCCTAATGTTAAATTCGAGAAGCGGTTGGTGAACCTGAAACCATCTTCTCAGTTCCAAGATGGCAAAAATAGAGGAGGAGCTGATTTGAAGATAAAGATGAAGAAGTCAAGATCAACCAAGCTTTCAGATATTGAGAGCTTGAGATCACCATCATCATCATCTTCATCACTACCTTTGAGAAAGAGCATGTCCCAGCCCGGAAAGCCACCGCCTCTCAATGTTCAAAGCATTGCAACAGCAGCAGCAGTCCCTCTGAAGCAGGCGCTGACCAAAACAAGTGATGGCTCAGCACCAAACTATATGAAGTCCACCAGCTGTTCTGATGCAAGGAAGGAGCAATCACAGGTTAGTGTTCGAAGTTCTCCGGTTATTTCAGATAGTAAGAATCAAAACCGAAGAAATTTGAGCAATTCAAAACTTGGCTCTGGCTCTAACACTAAATCAGCTAGGACTTCTAGTCTGAAGCTGGTGAGAACTTTAATAAAGTCCCCTAGTTTTAAGCATGCAAGAGCTTCAGCCAAGAAAACTTCCAGGGTTGCTCTTTGTGAAGATGTGAGTGTTCAGAAAGCAACTTGTTCTTCAACACTGAAGGACTCAAAGTTTCCTAAGTATCTGATGATTAGTCCTGGCGGAACGGAAGCTGAGGGAACTTCAATGATGAAGGTCTGCCCCTATACTTACTGCTCTCTTAATGGACATCATCATTCACCTGTGACTCCACTGAAGTGTTTCTTGTCGTCAAGAAGACGGTCTGTGAAGAACCAGAAGATGGTGAAATTGCAAGCTCTAAGTCCTCGTAAAGCAAAGCCATCTAATTTGGGAATGAAGGAAAATGATTCCAAGCAAATGTTGTATGTTGACAATGAAAAACCTGCTCAAGAAGAAGTCGGGATCGAATTCTTTGTTGAAATATTTGCTAAAAGAAAGGAAGATGATGCTCAAACAATTGTTGAGAATGTACCTGAAGCAGAAATCATAGATAGCTTTCCTGAAGAGGAGCAAAAAGAAGATGTGGCTGATGAAGAATACCAACCTTCTTTGGATCAAGAAGAACCTGCCATGCGAAGCTGTTCGAGTGAGAGTGACTCCGATGGACTTTCGAGCGTTGAAGTAGATTATGCCAATTCTGAAGCAACTGACATGGAGTGGGAGGAAGGGCAATTTTCCCTTGCAGTGCTGTATGATGATGATGATGATGATGAATCTGGCTCAAGTGGTGGGTTTTCATCAATTCAAGATGGTGATATGCATGAAGAACCTGTGATCAAGTCTGATGCCATTGTTATTAACTGCAATGACATCATTCATGATTACTATGAAGTACTGCAAGAACTTTTCACAGAGAAAACTTCATCTTTTGAAACTCAACTAAAAAATGATGGCTCAAAACAGAATTTCGGCATTGAAGAATCTGAAAGATTGAGCTATGACCAATTTTCTTACTCTGAGGATTCATTTGAAGAAGACAGTGAACTATCAGAGACAGAAATAGAGATATCTTCATCTTCAATACAAGAGCCAATGGAGGACCTAACAGCAACTGGTGAGGAAGTTCAAGAGAAAACTGGAATGGTGTCCCATCTTGGAGATGTTGAAAGCCACTACACTGCTGCTTGTGAGACAGCTGAAGCTTCAGATAGCCAACCAAGGAATGAATTTCAAGATGACGGAACAACTATGTCGACAAGTAACCGAATTTCTAATGCCGCTCAGGATATCAGCACAACATATGAAGCTGAAACAAATGCAGAGAAAGAGAAATCAGAAACAGACCAAAAAGCTGTCACTTCTGTTTCCGGAAATCAAATGGAAGAGGCACAGAAAGTTGAAGACACCGAATCCTCAGCAGATACCCAGATTTCTGATTCAGGAAAAACTATGGTTGTACATGATGAAGATGCTAGTAAGGTTGGTGATAATGTGGACACTGCTGAAGATTGTAATTCAGGCCAAGGTATTGCTGATGAAAACACTTTTGTTGAGAGTAAAGATAACCTGGCTAATGGGGAGTACACGGATAATGCTAAAGCTGAGAATCATAGCTTCACAGAGAAGGATCAAAGTGAATTTAAGAAGAAAAAGATCTCAAATTCCAGTGCAGGTGAGGATCAGAGTGATCAAAAGCTGAAGCAAATAGGTGTAGCAGAAAACAGTGGTGGAGACATTGATGGAATGGAAGTAGACAACAATTCAGGGCCAGATGCAGCCGAAACTTTTCGTAAGGCCAATAGTAGCATCAGTCCAGGGATGAAGAGAAAGTTTTCCCATCAAGAAAGCAATTCTGACGAAGAACTCTTAAACACAAACCGGAAATGGAAAATCAACTGCAAGAGAACTATCAAGGATGAGGAAGAACAATGGAAATACAATCAGAGAAAACCCAATTACCTGCCGTTGGTTCCTGGCCCTGAAGCAGAGAAGGTTGACTTGAGGCATCAGATGATGGATGAAAAGAAAAATGCAGAGGAATGGATGCTCGACTTTGCAATCCAACAAGCTGTCACCAAACTTGCTCCAGCTAGGAAGAAGAAGGTGGCATTGCTTGTTGAAGCTTTGAAAAAGTCATGCCAGTGCCGCAATATGAACCCCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

879

Amino Acids

97.33

Weight (kDa)

4.88

Isoelectric Point (pI)

54.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 773 - 866 7e-25 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2135
Acc36I ACCTGC 4 cut(s) 925, 1108, 2135, 2442
AccB7I CCANNNNNTGG 1 cut(s) 814
AccBSI CCGCTC 1 cut(s) 1754
AccI GTMKAC 2 cut(s) 1727, 2226
AccIII TCCGGA 1 cut(s) 1841
AciI CCGC 5 cut(s) 67, 254, 675, 1752, 2620
AclWI GGATC 5 cut(s) 38, 944, 1095, 2109, 2160
AcsI RAATTY 8 cut(s) 56, 445, 941, 1438, 1700, 1740, 2111, 2134
AfaI GTAC 5 cut(s) 18, 1005, 1368, 1932, 2057
AfiI CCNNNNNNNGG 3 cut(s) 13, 814, 2440
AhdI GACNNNNNGTC 1 cut(s) 501
AjnI CCWGG 5 cut(s) 575, 670, 2040, 2287, 2446
AleI CACNNNNGTG 1 cut(s) 769
Alw26I GTCTC 3 cut(s) 1520, 1658, 2199
AlwI GGATC 5 cut(s) 38, 944, 1095, 2109, 2160
AlwNI CAGNNNCTG 2 cut(s) 310, 1586
Aor13HI TCCGGA 1 cut(s) 1841
AoxI GGCC 4 cut(s) 1993, 2240, 2268, 2449
ApeKI GCWGC 4 cut(s) 284, 287, 1655, 2249
ApoI RAATTY 8 cut(s) 56, 445, 941, 1438, 1700, 1740, 2111, 2134
Asp700I GAANNNNTTC 4 cut(s) 773, 1114, 2259, 2305
AspLEI GCGC 1 cut(s) 310
AspS9I GGNCC 4 cut(s) 4, 1573, 2240, 2450
AsuC2I CCSGG 1 cut(s) 243
AsuII TTCGAA 1 cut(s) 400
AvaII GGWCC 2 cut(s) 4, 1573
BbsI GAAGAC 3 cut(s) 796, 1515, 1881
BbvCI CCTCAGC 2 cut(s) 687, 1888
BbvI GCAGC 4 cut(s) 296, 299, 1642, 2261
BceAI ACGGC 1 cut(s) 2422
BciT130I CCWGG 5 cut(s) 577, 672, 2042, 2289, 2448
BclI TGATCA 2 cut(s) 1308, 2161
BcnI CCSGG 1 cut(s) 243
BcoDI GTCTC 3 cut(s) 1520, 1658, 2199
BfaI CTAG 5 cut(s) 492, 501, 537, 1947, 2565
BfoI RGCGCY 1 cut(s) 311
BfuAI ACCTGC 4 cut(s) 925, 1108, 2135, 2442
BglII AGATCT 1 cut(s) 2127
BisI GCNGC 6 cut(s) 285, 288, 1656, 1752, 2250, 2620
BlpI GCTNAGC 1 cut(s) 331
BlsI GCNGC 6 cut(s) 286, 289, 1657, 1753, 2251, 2621
BmcAI AGTACT 1 cut(s) 1368
Bme1390I CCNGG 6 cut(s) 243, 577, 672, 2042, 2289, 2448
Bme18I GGWCC 2 cut(s) 4, 1573
BmeRI GACNNNNNGTC 1 cut(s) 501
BmgT120I GGNCC 4 cut(s) 4, 1573, 2240, 2450
BmiI GGNNCC 1 cut(s) 2445
BmrFI CCNGG 6 cut(s) 243, 577, 672, 2042, 2289, 2448
BmsI GCATC 8 cut(s) 358, 970, 1309, 1933, 2236, 2289, 2488, 2508
BpiI GAAGAC 3 cut(s) 796, 1515, 1881
BplI GAGNNNNNCTC 2 cut(s) 1115, 1147
BpmI CTGGAG 1 cut(s) 2544
Bpu10I CCTNAGC 3 cut(s) 687, 1755, 1888
Bpu1102I GCTNAGC 1 cut(s) 331
Bpu14I TTCGAA 1 cut(s) 400
BpuEI CTTGAG 4 cut(s) 205, 906, 1240, 2494
BpuMI CCSGG 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 193
BsaJI CCNNGG 4 cut(s) 576, 1691, 1996, 2288
BsaWI WCCGGW 3 cut(s) 409, 1841, 2352
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 814, 2440
Bse1I ACTGG 4 cut(s) 1591, 1615, 2139, 2614
Bse3DI GCAATG 3 cut(s) 273, 1345, 2582
Bse8I GATNNNNATC 1 cut(s) 193
BseAI TCCGGA 1 cut(s) 1841
BseBI CCWGG 5 cut(s) 577, 672, 2042, 2289, 2448
BseDI CCNNGG 4 cut(s) 576, 1691, 1996, 2288
BseGI GGATG 4 cut(s) 2298, 2395, 2497, 2523
BseJI GATNNNNATC 1 cut(s) 193
BseLI CCNNNNNNNGG 3 cut(s) 13, 814, 2440
BseMI GCAATG 3 cut(s) 273, 1345, 2582
BseMII CTCAG 7 cut(s) 107, 345, 678, 1482, 1769, 1902, 2067
BseNI ACTGG 4 cut(s) 1591, 1615, 2139, 2614
BseRI GAGGAG 3 cut(s) 132, 135, 1055
BseXI GCAGC 4 cut(s) 296, 299, 1642, 2261
BseYI CCCAGC 1 cut(s) 237
BsgI GTGCAG 1 cut(s) 2163
BshFI GGCC 4 cut(s) 1995, 2242, 2270, 2451
BsiSI CCGG 4 cut(s) 243, 410, 1842, 2353
BslFI GGGAC 4 cut(s) 220, 278, 517, 1606
BslI CCNNNNNNNGG 3 cut(s) 13, 814, 2440
BsmAI GTCTC 3 cut(s) 1520, 1658, 2199
BsmFI GGGAC 4 cut(s) 220, 278, 517, 1606
BsnI GGCC 4 cut(s) 1995, 2242, 2270, 2451
Bsp119I TTCGAA 1 cut(s) 400
Bsp13I TCCGGA 1 cut(s) 1841
Bsp1407I TGTACA 1 cut(s) 1930
Bsp1720I GCTNAGC 1 cut(s) 331
BspACI CCGC 5 cut(s) 67, 254, 675, 1752, 2620
BspANI GGCC 4 cut(s) 1995, 2242, 2270, 2451
BspCNI CTCAG 7 cut(s) 106, 344, 679, 1483, 1768, 1901, 2068
BspEI TCCGGA 1 cut(s) 1841
BspHI TCATGA 1 cut(s) 1351
BspLI GGNNCC 1 cut(s) 2445
BspMI ACCTGC 4 cut(s) 925, 1108, 2135, 2442
BspPI GGATC 5 cut(s) 38, 944, 1095, 2109, 2160
BspT104I TTCGAA 1 cut(s) 400
BsrBI CCGCTC 1 cut(s) 1754
BsrDI GCAATG 3 cut(s) 273, 1345, 2582
BsrGI TGTACA 1 cut(s) 1930
BsrI ACTGG 4 cut(s) 1591, 1615, 2139, 2614
BssECI CCNNGG 4 cut(s) 576, 1691, 1996, 2288
BssT1I CCWWGG 2 cut(s) 1691, 1996
Bst2UI CCWGG 5 cut(s) 577, 672, 2042, 2289, 2448
Bst4CI ACNGT 3 cut(s) 795, 1514, 2198
Bst6I CTCTTC 3 cut(s) 1032, 1851, 2291
BstAUI TGTACA 1 cut(s) 1930
BstBI TTCGAA 1 cut(s) 400
BstC8I GCNNGC 4 cut(s) 30, 306, 549, 828
BstDEI CTNAG 9 cut(s) 93, 331, 651, 687, 833, 1491, 1755, 1888, 2076
BstF5I GGATG 4 cut(s) 2298, 2395, 2497, 2523
BstH2I RGCGCY 1 cut(s) 311
BstHHI GCGC 1 cut(s) 310
BstMAI GTCTC 3 cut(s) 1520, 1658, 2199
BstMWI GCNNNNNNNGC 4 cut(s) 38, 247, 284, 2619
BstNI CCWGG 5 cut(s) 577, 672, 2042, 2289, 2448
BstNSI RCATGY 2 cut(s) 235, 551
BstSCI CCNGG 6 cut(s) 241, 575, 670, 2040, 2287, 2446
BstV1I GCAGC 4 cut(s) 296, 299, 1642, 2261
BstV2I GAAGAC 3 cut(s) 796, 1515, 1881
BstX2I RGATCY 2 cut(s) 43, 2127
BstYI RGATCY 2 cut(s) 43, 2127
BsuRI GGCC 4 cut(s) 1995, 2242, 2270, 2451
BtsCI GGATG 4 cut(s) 2298, 2395, 2497, 2523
BtsI GCAGTG 3 cut(s) 1224, 1650, 1971
BtsIMutI CAGTG 9 cut(s) 629, 764, 1224, 1519, 1650, 1971, 2146, 2203, 2621
BveI ACCTGC 4 cut(s) 925, 1108, 2135, 2442
Cac8I GCNNGC 4 cut(s) 30, 306, 549, 828
CaiI CAGNNNCTG 2 cut(s) 310, 1586
CciI TCATGA 1 cut(s) 1351
CfoI GCGC 1 cut(s) 310
Cfr13I GGNCC 4 cut(s) 4, 1573, 2240, 2450
Csp6I GTAC 5 cut(s) 17, 1004, 1367, 1931, 2056
CviAII CATG 8 cut(s) 232, 548, 1105, 1186, 1295, 1352, 1934, 2610
CviQI GTAC 5 cut(s) 17, 1004, 1367, 1931, 2056
DdeI CTNAG 9 cut(s) 93, 331, 651, 687, 833, 1491, 1755, 1888, 2076
DriI GACNNNNNGTC 1 cut(s) 501
Eam1104I CTCTTC 3 cut(s) 1032, 1851, 2291
Eam1105I GACNNNNNGTC 1 cut(s) 501
EarI CTCTTC 3 cut(s) 1032, 1851, 2291
EciI GGCGGA 1 cut(s) 690
Eco130I CCWWGG 2 cut(s) 1691, 1996
Eco32I GATATC 2 cut(s) 1542, 1762
Eco47I GGWCC 2 cut(s) 4, 1573
EcoO109I RGGNCCY 1 cut(s) 1573
EcoRI GAATTC 1 cut(s) 941
EcoRII CCWGG 5 cut(s) 575, 670, 2040, 2287, 2446
EcoRV GATATC 2 cut(s) 1542, 1762
EcoT14I CCWWGG 2 cut(s) 1691, 1996
EcoT22I ATGCAT 1 cut(s) 1296
ErhI CCWWGG 2 cut(s) 1691, 1996
FaeI CATG 8 cut(s) 235, 551, 1108, 1189, 1298, 1355, 1937, 2613
FalI AAGNNNNNCTT 4 cut(s) 761, 793, 2456, 2488
FaqI GGGAC 4 cut(s) 220, 278, 517, 1606
FatI CATG 8 cut(s) 231, 547, 1104, 1185, 1294, 1351, 1933, 2609
FauNDI CATATG 1 cut(s) 1774
FbaI TGATCA 2 cut(s) 1308, 2161
FblI GTMKAC 2 cut(s) 1727, 2226
Fnu4HI GCNGC 6 cut(s) 285, 288, 1656, 1752, 2250, 2620
FokI GGATG 4 cut(s) 2305, 2402, 2504, 2530
Fsp4HI GCNGC 6 cut(s) 285, 288, 1656, 1752, 2250, 2620
FspBI CTAG 5 cut(s) 492, 501, 537, 1947, 2565
GlaI GCGC 1 cut(s) 309
GluI GCNGC 6 cut(s) 285, 288, 1656, 1752, 2250, 2620
GsaI CCCAGC 1 cut(s) 241
GsuI CTGGAG 1 cut(s) 2544
HaeII RGCGCY 1 cut(s) 311
HaeIII GGCC 4 cut(s) 1995, 2242, 2270, 2451
HapII CCGG 4 cut(s) 243, 410, 1842, 2353
HhaI GCGC 1 cut(s) 310
Hin1II CATG 8 cut(s) 235, 551, 1108, 1189, 1298, 1355, 1937, 2613
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HincII GTYRAC 3 cut(s) 904, 1728, 2470
HindII GTYRAC 3 cut(s) 904, 1728, 2470
HindIII AAGCTT 3 cut(s) 164, 1674, 2595
HpaII CCGG 4 cut(s) 243, 410, 1842, 2353
Hpy166II GTNNAC 9 cut(s) 76, 354, 904, 1517, 1728, 1969, 2058, 2227, 2470
Hpy8I GTNNAC 9 cut(s) 76, 354, 904, 1517, 1728, 1969, 2058, 2227, 2470
HpyAV CCTTC 9 cut(s) 370, 628, 700, 865, 1089, 1193, 2092, 2458, 2569
HpyCH4III ACNGT 3 cut(s) 795, 1514, 2198
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 247, 284, 2619
HpyF3I CTNAG 9 cut(s) 93, 331, 651, 687, 833, 1491, 1755, 1888, 2076
Hsp92II CATG 8 cut(s) 235, 551, 1108, 1189, 1298, 1355, 1937, 2613
HspAI GCGC 1 cut(s) 308
Kpn2I TCCGGA 1 cut(s) 1841
Ksp22I TGATCA 2 cut(s) 1308, 2161
LmnI GCTCC 4 cut(s) 122, 379, 1042, 2563
Lsp1109I GCAGC 4 cut(s) 296, 299, 1642, 2261
LweI GCATC 8 cut(s) 358, 970, 1309, 1933, 2236, 2289, 2488, 2508
MaeI CTAG 5 cut(s) 492, 501, 537, 1947, 2565
MaeIII GTNAC 5 cut(s) 757, 1127, 1733, 1828, 2545
MbiI CCGCTC 1 cut(s) 1754
MfeI CAATTG 1 cut(s) 990
MflI RGATCY 2 cut(s) 43, 2127
MlyI GAGTC 3 cut(s) 632, 754, 1124
MmeI TCCRAC 1 cut(s) 2560
Mph1103I ATGCAT 1 cut(s) 1296
MroI TCCGGA 1 cut(s) 1841
MroXI GAANNNNTTC 4 cut(s) 773, 1114, 2259, 2305
MseI TTAA 7 cut(s) 54, 524, 543, 735, 1332, 2115, 2342
MslI CAYNNNNRTG 2 cut(s) 769, 2614
MspA1I CMGCKG 2 cut(s) 360, 1670
MspI CCGG 4 cut(s) 243, 410, 1842, 2353
MspR9I CCNGG 6 cut(s) 243, 577, 672, 2042, 2289, 2448
MunI CAATTG 1 cut(s) 990
MvaI CCWGG 5 cut(s) 577, 672, 2042, 2289, 2448
MwoI GCNNNNNNNGC 4 cut(s) 38, 247, 284, 2619
NciI CCSGG 1 cut(s) 243
NdeI CATATG 1 cut(s) 1774
NlaIII CATG 8 cut(s) 235, 551, 1108, 1189, 1298, 1355, 1937, 2613
NlaIV GGNNCC 1 cut(s) 2445
NmuCI GTSAC 4 cut(s) 757, 1127, 1828, 2545
NsiI ATGCAT 1 cut(s) 1296
NspI RCATGY 2 cut(s) 235, 551
NspV TTCGAA 1 cut(s) 400
OliI CACNNNNGTG 1 cut(s) 769
PaeI GCATGC 1 cut(s) 551
PagI TCATGA 1 cut(s) 1351
PaqCI CACCTGC 1 cut(s) 2135
PdmI GAANNNNTTC 4 cut(s) 773, 1114, 2259, 2305
PfeI GAWTC 8 cut(s) 430, 881, 1247, 1454, 1496, 1883, 1910, 2080
PflMI CCANNNNNTGG 1 cut(s) 814
PkrI GCNGC 6 cut(s) 286, 289, 1657, 1753, 2251, 2621
PleI GAGTC 3 cut(s) 632, 754, 1124
PpsI GAGTC 3 cut(s) 632, 754, 1124
PpuMI RGGWCCY 1 cut(s) 1573
Psp5II RGGWCCY 1 cut(s) 1573
Psp6I CCWGG 5 cut(s) 575, 670, 2040, 2287, 2446
PspFI CCCAGC 1 cut(s) 237
PspGI CCWGG 5 cut(s) 575, 670, 2040, 2287, 2446
PspN4I GGNNCC 1 cut(s) 2445
PspPI GGNCC 4 cut(s) 4, 1573, 2240, 2450
PspPPI RGGWCCY 1 cut(s) 1573
PstNI CAGNNNCTG 2 cut(s) 310, 1586
PsuI RGATCY 2 cut(s) 43, 2127
PvuII CAGCTG 2 cut(s) 360, 1670
RsaI GTAC 5 cut(s) 18, 1005, 1368, 1932, 2057
RsaNI GTAC 5 cut(s) 17, 1004, 1367, 1931, 2056
RseI CAYNNNNRTG 2 cut(s) 769, 2614
SalI GTCGAC 1 cut(s) 1726
SaqAI TTAA 7 cut(s) 54, 524, 543, 735, 1332, 2115, 2342
SatI GCNGC 6 cut(s) 285, 288, 1656, 1752, 2250, 2620
Sau96I GGNCC 4 cut(s) 4, 1573, 2240, 2450
ScaI AGTACT 1 cut(s) 1368
SchI GAGTC 3 cut(s) 632, 754, 1124
ScrFI CCNGG 6 cut(s) 243, 577, 672, 2042, 2289, 2448
SfaNI GCATC 8 cut(s) 358, 970, 1309, 1933, 2236, 2289, 2488, 2508
SfuI TTCGAA 1 cut(s) 400
SinI GGWCC 2 cut(s) 4, 1573
SmiMI CAYNNNNRTG 2 cut(s) 769, 2614
SmlI CTYRAG 4 cut(s) 184, 921, 1255, 2473
SmoI CTYRAG 4 cut(s) 184, 921, 1255, 2473
SphI GCATGC 1 cut(s) 551
SsiI CCGC 5 cut(s) 67, 254, 675, 1752, 2620
SspI AATATT 1 cut(s) 957
SspMI CTAG 5 cut(s) 492, 501, 537, 1947, 2565
StyD4I CCNGG 6 cut(s) 241, 575, 670, 2040, 2287, 2446
StyI CCWWGG 2 cut(s) 1691, 1996
TaaI ACNGT 3 cut(s) 795, 1514, 2198
TaqI TCGA 7 cut(s) 60, 400, 939, 1118, 1145, 1727, 2523
TatI WGTACW 3 cut(s) 1366, 1930, 2055
TauI GCSGC 2 cut(s) 1754, 2622
TfiI GAWTC 8 cut(s) 430, 881, 1247, 1454, 1496, 1883, 1910, 2080
Tru1I TTAA 7 cut(s) 54, 524, 543, 735, 1332, 2115, 2342
Tru9I TTAA 7 cut(s) 54, 524, 543, 735, 1332, 2115, 2342
TscAI CASTG 9 cut(s) 636, 771, 1224, 1519, 1657, 1978, 2146, 2203, 2621
TseFI GTSAC 4 cut(s) 757, 1127, 1828, 2545
TseI GCWGC 4 cut(s) 284, 287, 1655, 2249
Tsp45I GTSAC 4 cut(s) 757, 1127, 1828, 2545
TspGWI ACGGA 3 cut(s) 695, 1728, 2075
TspRI CASTG 9 cut(s) 636, 771, 1224, 1519, 1657, 1978, 2146, 2203, 2621
Van91I CCANNNNNTGG 1 cut(s) 814
VpaK11BI GGWCC 2 cut(s) 4, 1573
XapI RAATTY 8 cut(s) 56, 445, 941, 1438, 1700, 1740, 2111, 2134
XceI RCATGY 2 cut(s) 235, 551
XmiI GTMKAC 2 cut(s) 1727, 2226
XmnI GAANNNNTTC 4 cut(s) 773, 1114, 2259, 2305
XspI CTAG 5 cut(s) 492, 501, 537, 1947, 2565
ZrmI AGTACT 1 cut(s) 1368
Zsp2I ATGCAT 1 cut(s) 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.