Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0003606

Physical Location & Seq

Genomic Coordinates Reverse (-)

14838041 .. 14843908

Gene Structure 5868 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0178800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2595 bp

ATGGCTAATCCTACTTACCAGGAATCCTTGTCATTTGGAGGCCCAGACCATCCTCAAGGTCAAGAAGTTATTCCATTTGAGAGTGGAACCCTGAAGGTTTTGCTACATGGCTCCCTAGTCATATGGGTCAAGAAGGCCGAAAACCTTCCTGACATGGAGAGTTTTCGCAAGCATGTGGGGCATGCAATTGCGAAGTTGCCTGGGACGAGATGCAAAAATATAATCAGTTCTCCATATGTTACGATTTCTTTATCAAATGCTGTGATTGGGAGGACCTTTGTGATGAGTAGTACCAAGAACCCTGTGTGGGAGCAACATTTTGATGTTCCTGTTGCTCATTATGCTTCACGAGTGAACTTTGTTGTGAAACACAGTGATGTTTTCGGCTCTAAGACCATAGGTTTTGTTGGAATCTCAGCTCAACAGTTGTACTCTGGCATCAAAGTTGAGGGAACCTTCCCTATCCTTGACACTACCAGTGGTAAAAGCTGTATGCCTGGAGCTGTGTTGAGCTTGTCAATTCAGTATACCGCGATTGAAAAAATGGCATTTTATAATCGCGGAGTAGGTTTAGATCCTGATCATCAAGGGGTCCCTGGTACTTACTTTCCTGTTAGGAAAGGCGGTAAAGTTACTCTTTATCAAGATGCTCATGTTCCTGATGGTTGCCTCCCCAACTTCAAGCTTGACGGCGGTCTTAAGTATGAGCATGGAAATTGTTGGCAAGACATATTTGATGCAATAAGTCAGGCCCGCCGTTTGATATACATAGCTGGGTGGTCACTGCACCCCAAAGTCAGATTGCTTCTGAATAATAATGGTTCATCAGATCGCACATTGGGTGATCTGCTGAAGACCAAGTCACAGGAAGGTGTGAAAGTTCTGCTTCTTGTTTGGGATGATCCAACTTCTATTAAGGGATTTAAAATGGAAGGGCTCATGCATACCAATGATGAAGATGCTCGACGTTTCTTTAAGGACTCTTCAGTTCAATTTCTACTCTGCCCTCGATCATCAGTCCAAAGCTGGGGGAAAAAGATGGAAGCTGGAATAGTCTATACCCATCATCAGAAAACTGTAATTGTGGACGCTGATGCTGGTCATTTTAAAAGAAAGATCATTGCATTTGTTGGAGGTCTTGATTTGTGCTTGGGACGTTATGATACTCCGGAGCATTCTATATTTAGGACCTTACGTACAGTGCACAAGGATGACAATCGTAACCCGACTTTCTCGAAACCCGTTATTGGGTGTCCAAGGCAGCCATGGCATGATTTGCACAGTAAAATTGATGGTCCAGCTGCTTATGACATCCTCACCAATTTTGAGCAGCGTTGGGGGATGGCTTCAAAACCACATGGTCTTCAAAAACTAAAAGAACGAGGACTTCAAAAACTGAAATTACGAGGAGATGATGATGATGCATTACCCAAGATTGAGAAAATTCCTGAAATTCTTGGGATGACAGAGGTTCCTCGCTTGAGTGAGGATGATAAAGAAGCTTGGGATATTCAGGTTTTCCGTTCAATTGATTCCCATTCAGTGCAAGGATTTCCCGAAGACACTGGAGAAGCTACAAGGATGAACCTTGTTTGTGGGAAAAATGTGCTGATAGACATGAGTATACATGCAGCATATGTAAAAGCAATCCGTGCGGCTCAACATTTTATTTACATAGAGAACCAGTACTTTCTTGGATCATCATATAATTGGAAGTCAGACATTAGTGATACAGGTGCAAACAATTTGATACCGATGGAAATCGCTCTGAAAATAGCAAACAAAATCAAAGCAAATGAGAAGTTTTCTGTATATATTGTCATCCCCATGTGGCCAGAAGGTGATCCCAATACTATGCCTCTTCAAAGAATTCTCCTTTGGCAGCATAAAACAATGCAAATGATGTATGAAATGATTTACAAGGCTCTAAAGGAGGTTGGACTCGAAAATAAGTACTGTCCACAAGACTATTTGAATTTCTTTTGCCTCGGAAATCGTGAGAAACCAAGTGGAGATGCTGCAAATAATTCAAGTGCAGCAAACACTCCTGAAGTACTCACCCAGAATAGTCGGCGATTTATGATTTATGTCCATTCAAAAGGAATGATAGTGGATGATGAATACGTTATAGTGGGATCTGCAAATATCAATCAGCGATCCATGGAAGGTCTCCGAGATACTGAAATAGCAATGGGGGCTTATCAGCCTCATCATACTTGGGCGAGAAAACTCTCAAGTCCACACGGAAAGATATATGGATACAGAATGTCACTGTGGGCAGAGCATTTAGGACTTGTTGAAGAATGTTTCAATCAGCCAGAGAGTCTTGAATGTGTTCAAAGGGTAAGGTCTTTGGGTGAGCAGAACTGGAGTCAATACGTGGCCGACGAGGTTACAGAAATGAAGGGTCACCTCCTCAAGTATCCTGTTGAAGTTGGTCCAACGGGCACGGTGGGGCCTCTTCCGGGGTGTCCAACATTTCCAGACGTTGGTGGCAAAATATTGGGGAGTCTCGGCGACATAAAAATGAAGAGTCTGAGTATCAGTAGCAGGGGGAGGTCAATATCACTTAGAGGCGCCATCATCACCACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaM_binding

Protein Analysis

864

Amino Acids

96.62

Weight (kDa)

8.34

Isoelectric Point (pI)

36.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	37 - 154	9.9e-13	C2 domain
PLDc_2	PF13091	682 - 731	2.3e-06	PLD-like domain
PLDc	PF00614	695 - 720	2.8e-08	Phospholipase D Active site motif
PLD_C	PF12357	767 - 837	4.6e-30	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38800 AT3G54570
fragaria_vesca	FvH4_5g29010 FvH4_5g29010
malus_domestica	MD00G1188700.v1.1 MD02G1294400.v1.1 MD02G1294500.v1.1 MD07G1032000.v1.1 MD07G1032600.v1.1
prunus_persica	Prupe.2G029500_v2.0.a1 Prupe.2G029500_v2.0.a1 Prupe.2G029500_v2.0.a1 Prupe.2G029500_v2.0.a1
pyrus_communis	pycom02g24790
rosa_chinensis	RchiOBHm_Chr1g0319711
rosa_laevigata	RLG00000002328 RLG00000002329 RLG00000030462
rosa_multiflora	Rmu_sc0001301.1_g000023 Rmu_sc0002539.1_g000113
rosa_roxburghii	Rroxscaffold_3G00240740 Rroxscaffold_3G00240750 Rroxscaffold_4G00328170
rosa_rugosa	Rorug01G0029600 Rorug07G0178800 Rorug07G0178900
rosa_samantha	Rh1AG041500 Rh1BG038800 Rh1CG042400 Rh1DG027200 Rh7BG311900 Rh7BG312000 Rh7CG336800
rosa_wichuraiana	Rw1G003450 Rw7G027250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	555
AccB1I	GGYRCC	1 cut(s)	2576
AccB7I	CCANNNNNTGG	1 cut(s)	2489
AccI	GTMKAC	2 cut(s)	527, 1624
AccII	CGCG	2 cut(s)	533, 561
AccIII	TCCGGA	1 cut(s)	1168
AciI	CCGC	6 cut(s)	531, 561, 624, 693, 754, 1655
AclWI	GGATC	6 cut(s)	569, 896, 1705, 1838, 2143, 2151
AcoI	YGGCCR	2 cut(s)	1832, 2382
AcsI	RAATTY	4 cut(s)	1443, 1452, 1869, 1975
AcuI	CTGAAG	4 cut(s)	113, 872, 969, 2070
AcyI	GRCGYC	1 cut(s)	2577
AfaI	GTAC	7 cut(s)	292, 431, 601, 1198, 1688, 1955, 2055
AfiI	CCNNNNNNNGG	6 cut(s)	307, 1027, 1247, 1248, 2465, 2489
AflII	CTTAAG	1 cut(s)	698
AjnI	CCWGG	4 cut(s)	18, 199, 496, 595
AjuI	GAANNNNNNNTTGG	2 cut(s)	1861, 1893
Alw21I	GWGCWC	1 cut(s)	1206
Alw26I	GTCTC	2 cut(s)	2174, 2516
Alw44I	GTGCAC	1 cut(s)	1202
AlwI	GGATC	6 cut(s)	569, 896, 1705, 1838, 2143, 2151
Aor13HI	TCCGGA	1 cut(s)	1168
AoxI	GGCC	6 cut(s)	40, 135, 750, 1832, 2382, 2456
ApaLI	GTGCAC	1 cut(s)	1202
ApeKI	GCWGC	7 cut(s)	1261, 1301, 1330, 1631, 1882, 2018, 2036
ApoI	RAATTY	4 cut(s)	1443, 1452, 1869, 1975
Asp700I	GAANNNNTTC	3 cut(s)	69, 144, 2334
AspLEI	GCGC	1 cut(s)	2579
AspS9I	GGNCC	8 cut(s)	41, 273, 592, 751, 1188, 1295, 2438, 2456
AsuC2I	CCSGG	1 cut(s)	2466
AsuHPI	GGTGA	7 cut(s)	854, 1309, 1853, 2050, 2369, 2402, 2578
AvaII	GGWCC	5 cut(s)	273, 592, 1188, 1295, 2438
BaeGI	GKGCMC	2 cut(s)	1206, 2450
BalI	TGGCCA	1 cut(s)	1834
BanI	GGYRCC	1 cut(s)	2576
BanII	GRGCYC	1 cut(s)	939
BauI	CACGAG	1 cut(s)	348
BbsI	GAAGAC	3 cut(s)	860, 1355, 1566
Bbv12I	GWGCWC	1 cut(s)	1206
BbvI	GCAGC	7 cut(s)	1273, 1288, 1342, 1643, 1894, 2005, 2048
BccI	CCATC	8 cut(s)	57, 656, 1033, 1071, 1286, 1336, 1750, 2588
BceAI	ACGGC	2 cut(s)	706, 741
BciT130I	CCWGG	4 cut(s)	20, 201, 498, 597
BciVI	GTATCC	2 cut(s)	2252, 2433
BclI	TGATCA	1 cut(s)	580
BcnI	CCSGG	1 cut(s)	2466
BcoDI	GTCTC	2 cut(s)	2174, 2516
BfaI	CTAG	1 cut(s)	116
BfoI	RGCGCY	1 cut(s)	2580
BfrI	CTTAAG	1 cut(s)	698
BfuI	GTATCC	2 cut(s)	2252, 2433
BisI	GCNGC	8 cut(s)	1262, 1302, 1331, 1632, 1656, 1883, 2019, 2037
BlsI	GCNGC	8 cut(s)	1263, 1303, 1332, 1633, 1657, 1884, 2020, 2038
BmcAI	AGTACT	3 cut(s)	1688, 1955, 2055
Bme1390I	CCNGG	5 cut(s)	20, 201, 498, 597, 2466
Bme18I	GGWCC	5 cut(s)	273, 592, 1188, 1295, 2438
BmgT120I	GGNCC	8 cut(s)	41, 273, 592, 751, 1188, 1295, 2438, 2456
BmiI	GGNNCC	8 cut(s)	88, 112, 454, 593, 594, 1473, 2457, 2578
BmrFI	CCNGG	5 cut(s)	20, 201, 498, 597, 2466
BmsI	GCATC	8 cut(s)	200, 447, 637, 727, 949, 1084, 1411, 2005
BoxI	GACNNNNGTC	1 cut(s)	693
BpiI	GAAGAC	3 cut(s)	860, 1355, 1566
BpmI	CTGGAG	3 cut(s)	519, 1587, 2389
BpuEI	CTTGAG	4 cut(s)	39, 1501, 2218, 2402
BpuMI	CCSGG	1 cut(s)	2466
BsaAI	YACGTR	2 cut(s)	1196, 2380
BsaBI	GATNNNNATC	3 cut(s)	579, 1215, 1760
BsaHI	GRCGYC	1 cut(s)	2577
BsaI	GGTCTC	1 cut(s)	2174
BsaJI	CCNNGG	7 cut(s)	200, 595, 1256, 1265, 1987, 2160, 2465
BsaWI	WCCGGW	1 cut(s)	1168
Bsc4I	CCNNNNNNNGG	6 cut(s)	307, 1027, 1247, 1248, 2465, 2489
Bse1I	ACTGG	4 cut(s)	477, 1570, 1684, 2372
Bse3DI	GCAATG	2 cut(s)	1119, 2196
Bse8I	GATNNNNATC	3 cut(s)	579, 1215, 1760
BseAI	TCCGGA	1 cut(s)	1168
BseBI	CCWGG	4 cut(s)	20, 201, 498, 597
BseDI	CCNNGG	7 cut(s)	200, 595, 1256, 1265, 1987, 2160, 2465
BseJI	GATNNNNATC	3 cut(s)	579, 1215, 1760
BseLI	CCNNNNNNNGG	6 cut(s)	307, 1027, 1247, 1248, 2465, 2489
BseMI	GCAATG	2 cut(s)	1119, 2196
BseMII	CTCAG	2 cut(s)	429, 2528
BseNI	ACTGG	4 cut(s)	477, 1570, 1684, 2372
BseRI	GAGGAG	2 cut(s)	1422, 2405
BseSI	GKGCMC	2 cut(s)	1206, 2450
BseXI	GCAGC	7 cut(s)	1273, 1288, 1342, 1643, 1894, 2005, 2048
BseYI	CCCAGC	2 cut(s)	773, 1026
BsgI	GTGCAG	2 cut(s)	770, 2055
Bsh1236I	CGCG	2 cut(s)	533, 561
BshFI	GGCC	6 cut(s)	42, 137, 752, 1834, 2384, 2458
BshNI	GGYRCC	1 cut(s)	2576
BsiHKAI	GWGCWC	1 cut(s)	1206
BsiSI	CCGG	2 cut(s)	1169, 2465
BslFI	GGGAC	3 cut(s)	217, 578, 1167
BslI	CCNNNNNNNGG	6 cut(s)	307, 1027, 1247, 1248, 2465, 2489
BsmAI	GTCTC	2 cut(s)	2174, 2516
BsmFI	GGGAC	3 cut(s)	217, 578, 1167
BsmI	GAATGC	1 cut(s)	1174
BsnI	GGCC	6 cut(s)	42, 137, 752, 1834, 2384, 2458
Bso31I	GGTCTC	1 cut(s)	2174
Bsp1286I	GDGCHC	3 cut(s)	939, 1206, 2450
Bsp13I	TCCGGA	1 cut(s)	1168
Bsp19I	CCATGG	2 cut(s)	1265, 2160
BspACI	CCGC	6 cut(s)	531, 561, 624, 693, 754, 1655
BspANI	GGCC	6 cut(s)	42, 137, 752, 1834, 2384, 2458
BspCNI	CTCAG	2 cut(s)	428, 2529
BspEI	TCCGGA	1 cut(s)	1168
BspFNI	CGCG	2 cut(s)	533, 561
BspLI	GGNNCC	8 cut(s)	88, 112, 454, 593, 594, 1473, 2457, 2578
BspPI	GGATC	6 cut(s)	569, 896, 1705, 1838, 2143, 2151
BspT107I	GGYRCC	1 cut(s)	2576
BspTI	CTTAAG	1 cut(s)	698
BspTNI	GGTCTC	1 cut(s)	2174
BsrDI	GCAATG	2 cut(s)	1119, 2196
BsrI	ACTGG	4 cut(s)	477, 1570, 1684, 2372
BssECI	CCNNGG	7 cut(s)	200, 595, 1256, 1265, 1987, 2160, 2465
BssNAI	GTATAC	2 cut(s)	528, 1625
BssNI	GRCGYC	1 cut(s)	2577
BssSI	CACGAG	1 cut(s)	348
BssT1I	CCWWGG	3 cut(s)	1256, 1265, 2160
Bst1107I	GTATAC	2 cut(s)	528, 1625
Bst2BI	CACGAG	1 cut(s)	348
Bst2UI	CCWGG	4 cut(s)	20, 201, 498, 597
Bst4CI	ACNGT	8 cut(s)	374, 426, 1078, 1201, 1283, 1958, 2274, 2452
Bst6I	CTCTTC	4 cut(s)	988, 1866, 2466, 2525
BstACI	GRCGYC	1 cut(s)	2577
BstAFI	CTTAAG	1 cut(s)	698
BstAPI	GCANNNNNTGC	2 cut(s)	1276, 1652
BstBAI	YACGTR	2 cut(s)	1196, 2380
BstC8I	GCNNGC	3 cut(s)	170, 183, 754
BstDEI	CTNAG	4 cut(s)	390, 415, 2537, 2570
BstDSI	CCRYGG	2 cut(s)	1265, 2160
BstEII	GGTNACC	1 cut(s)	2408
BstFNI	CGCG	2 cut(s)	533, 561
BstH2I	RGCGCY	1 cut(s)	2580
BstHHI	GCGC	1 cut(s)	2579
BstMAI	GTCTC	2 cut(s)	2174, 2516
BstMWI	GCNNNNNNNGC	6 cut(s)	178, 341, 1267, 1276, 1652, 2195
BstNI	CCWGG	4 cut(s)	20, 201, 498, 597
BstNSI	RCATGY	3 cut(s)	176, 185, 1631
BstPAI	GACNNNNGTC	1 cut(s)	693
BstPI	GGTNACC	1 cut(s)	2408
BstSCI	CCNGG	5 cut(s)	18, 199, 496, 595, 2464
BstSLI	GKGCMC	2 cut(s)	1206, 2450
BstSNI	TACGTA	1 cut(s)	1196
BstUI	CGCG	2 cut(s)	533, 561
BstV1I	GCAGC	7 cut(s)	1273, 1288, 1342, 1643, 1894, 2005, 2048
BstV2I	GAAGAC	3 cut(s)	860, 1355, 1566
BstX2I	RGATCY	2 cut(s)	574, 2135
BstYI	RGATCY	2 cut(s)	574, 2135
BstZ17I	GTATAC	2 cut(s)	528, 1625
BsuI	GTATCC	2 cut(s)	2252, 2433
BsuRI	GGCC	6 cut(s)	42, 137, 752, 1834, 2384, 2458
BtgI	CCRYGG	2 cut(s)	1265, 2160
BtsI	GCAGTG	1 cut(s)	782
BtsIMutI	CAGTG	7 cut(s)	379, 484, 782, 1206, 1548, 1563, 2270
Cac8I	GCNNGC	3 cut(s)	170, 183, 754
CfoI	GCGC	1 cut(s)	2579
Cfr13I	GGNCC	8 cut(s)	41, 273, 592, 751, 1188, 1295, 2438, 2456
CseI	GACGC	1 cut(s)	1097
Csp6I	GTAC	7 cut(s)	291, 430, 600, 1197, 1687, 1954, 2054
CviQI	GTAC	7 cut(s)	291, 430, 600, 1197, 1687, 1954, 2054
DdeI	CTNAG	4 cut(s)	390, 415, 2537, 2570
DinI	GGCGCC	1 cut(s)	2578
DraI	TTTAAA	2 cut(s)	925, 1108
EaeI	YGGCCR	2 cut(s)	1832, 2382
Eam1104I	CTCTTC	4 cut(s)	988, 1866, 2466, 2525
EarI	CTCTTC	4 cut(s)	988, 1866, 2466, 2525
Eco105I	TACGTA	1 cut(s)	1196
Eco130I	CCWWGG	3 cut(s)	1256, 1265, 2160
Eco24I	GRGCYC	1 cut(s)	939
Eco31I	GGTCTC	1 cut(s)	2174
Eco47I	GGWCC	5 cut(s)	273, 592, 1188, 1295, 2438
Eco57I	CTGAAG	4 cut(s)	113, 872, 969, 2070
Eco91I	GGTNACC	1 cut(s)	2408
EcoO109I	RGGNCCY	4 cut(s)	273, 592, 1188, 2456
EcoO65I	GGTNACC	1 cut(s)	2408
EcoRI	GAATTC	1 cut(s)	1869
EcoRII	CCWGG	4 cut(s)	18, 199, 496, 595
EcoT14I	CCWWGG	3 cut(s)	1256, 1265, 2160
EcoT22I	ATGCAT	2 cut(s)	945, 1426
EcoT38I	GRGCYC	1 cut(s)	939
EgeI	GGCGCC	1 cut(s)	2578
EheI	GGCGCC	1 cut(s)	2578
ErhI	CCWWGG	3 cut(s)	1256, 1265, 2160
FalI	AAGNNNNNCTT	4 cut(s)	621, 653, 870, 902
FaqI	GGGAC	3 cut(s)	217, 578, 1167
FauI	CCCGC	1 cut(s)	761
FauNDI	CATATG	3 cut(s)	122, 235, 1636
FbaI	TGATCA	1 cut(s)	580
FblI	GTMKAC	2 cut(s)	527, 1624
Fnu4HI	GCNGC	8 cut(s)	1262, 1302, 1331, 1632, 1656, 1883, 2019, 2037
FriOI	GRGCYC	1 cut(s)	939
Fsp4HI	GCNGC	8 cut(s)	1262, 1302, 1331, 1632, 1656, 1883, 2019, 2037
FspBI	CTAG	1 cut(s)	116
GlaI	GCGC	1 cut(s)	2578
GluI	GCNGC	8 cut(s)	1262, 1302, 1331, 1632, 1656, 1883, 2019, 2037
GsaI	CCCAGC	2 cut(s)	777, 1030
GsuI	CTGGAG	3 cut(s)	519, 1587, 2389
HaeII	RGCGCY	1 cut(s)	2580
HaeIII	GGCC	6 cut(s)	42, 137, 752, 1834, 2384, 2458
HapII	CCGG	2 cut(s)	1169, 2465
HgaI	GACGC	1 cut(s)	1097
HhaI	GCGC	1 cut(s)	2579
Hin1I	GRCGYC	1 cut(s)	2577
Hin6I	GCGC	1 cut(s)	2577
HinP1I	GCGC	1 cut(s)	2577
HindIII	AAGCTT	2 cut(s)	683, 1500
HinfI	GANTC	9 cut(s)	23, 411, 980, 1532, 1941, 2323, 2371, 2509, 2533
HpaII	CCGG	2 cut(s)	1169, 2465
HphI	GGTGA	7 cut(s)	854, 1309, 1853, 2050, 2369, 2402, 2578
Hpy166II	GTNNAC	7 cut(s)	355, 528, 1087, 1204, 1625, 1961, 2240
Hpy188I	TCNGA	9 cut(s)	800, 810, 829, 1071, 1720, 1770, 1991, 2174, 2538
Hpy8I	GTNNAC	7 cut(s)	355, 528, 1087, 1204, 1625, 1961, 2240
Hpy99I	CGWCG	2 cut(s)	969, 2390
HpyAV	CCTTC	9 cut(s)	88, 127, 155, 466, 863, 926, 1832, 2159, 2398
HpyCH4III	ACNGT	8 cut(s)	374, 426, 1078, 1201, 1283, 1958, 2274, 2452
HpyCH4IV	ACGT	6 cut(s)	967, 1156, 1195, 2124, 2379, 2487
HpyF10VI	GCNNNNNNNGC	6 cut(s)	178, 341, 1267, 1276, 1652, 2195
HpyF3I	CTNAG	4 cut(s)	390, 415, 2537, 2570
HpySE526I	ACGT	6 cut(s)	967, 1156, 1195, 2124, 2379, 2487
Hsp92I	GRCGYC	1 cut(s)	2577
HspAI	GCGC	1 cut(s)	2577
KasI	GGCGCC	1 cut(s)	2576
KflI	GGGWCCC	1 cut(s)	592
Kpn2I	TCCGGA	1 cut(s)	1168
Ksp22I	TGATCA	1 cut(s)	580
LmnI	GCTCC	4 cut(s)	116, 310, 500, 1171
Lsp1109I	GCAGC	7 cut(s)	1273, 1288, 1342, 1643, 1894, 2005, 2048
LweI	GCATC	8 cut(s)	200, 447, 637, 727, 949, 1084, 1411, 2005
MaeI	CTAG	1 cut(s)	116
MaeII	ACGT	6 cut(s)	967, 1156, 1195, 2124, 2379, 2487
MaeIII	GTNAC	8 cut(s)	238, 631, 780, 861, 1220, 2268, 2392, 2408
MboII	GAAGA	9 cut(s)	865, 968, 975, 1355, 1571, 1853, 2312, 2453, 2542
MfeI	CAATTG	2 cut(s)	186, 1527
MflI	RGATCY	2 cut(s)	574, 2135
MhlI	GDGCHC	3 cut(s)	939, 1206, 2450
MlsI	TGGCCA	1 cut(s)	1834
MluNI	TGGCCA	1 cut(s)	1834
Mly113I	GGCGCC	1 cut(s)	2577
MlyI	GAGTC	6 cut(s)	974, 1935, 2332, 2380, 2518, 2542
MmeI	TCCRAC	6 cut(s)	388, 929, 1111, 1918, 2465, 2498
Mox20I	TGGCCA	1 cut(s)	1834
Mph1103I	ATGCAT	2 cut(s)	945, 1426
MroI	TCCGGA	1 cut(s)	1168
MroXI	GAANNNNTTC	3 cut(s)	69, 144, 2334
MscI	TGGCCA	1 cut(s)	1834
MseI	TTAA	5 cut(s)	699, 915, 924, 975, 1107
MslI	CAYNNNNRTG	6 cut(s)	321, 375, 948, 1209, 1826, 2525
Msp20I	TGGCCA	1 cut(s)	1834
MspA1I	CMGCKG	1 cut(s)	1301
MspCI	CTTAAG	1 cut(s)	698
MspI	CCGG	2 cut(s)	1169, 2465
MspR9I	CCNGG	5 cut(s)	20, 201, 498, 597, 2466
MunI	CAATTG	2 cut(s)	186, 1527
Mva1269I	GAATGC	1 cut(s)	1174
MvaI	CCWGG	4 cut(s)	20, 201, 498, 597
MvnI	CGCG	2 cut(s)	533, 561
MwoI	GCNNNNNNNGC	6 cut(s)	178, 341, 1267, 1276, 1652, 2195
NarI	GGCGCC	1 cut(s)	2577
NciI	CCSGG	1 cut(s)	2466
NcoI	CCATGG	2 cut(s)	1265, 2160
NdeI	CATATG	3 cut(s)	122, 235, 1636
NlaIV	GGNNCC	8 cut(s)	88, 112, 454, 593, 594, 1473, 2457, 2578
NmeAIII	GCCGAG	1 cut(s)	2493
NmuCI	GTSAC	4 cut(s)	780, 861, 2268, 2408
NsiI	ATGCAT	2 cut(s)	945, 1426
NspI	RCATGY	3 cut(s)	176, 185, 1631
PaeI	GCATGC	1 cut(s)	185
PcsI	WCGNNNNNNNCGW	1 cut(s)	2385
PctI	GAATGC	1 cut(s)	1174
PdmI	GAANNNNTTC	3 cut(s)	69, 144, 2334
PfeI	GAWTC	3 cut(s)	23, 411, 1532
PflFI	GACNNNGTC	1 cut(s)	859
PflMI	CCANNNNNTGG	1 cut(s)	2489
PkrI	GCNGC	8 cut(s)	1263, 1303, 1332, 1633, 1657, 1884, 2020, 2038
PleI	GAGTC	6 cut(s)	974, 1935, 2331, 2379, 2517, 2541
PluTI	GGCGCC	1 cut(s)	2580
PpsI	GAGTC	6 cut(s)	974, 1935, 2331, 2379, 2517, 2541
Ppu21I	YACGTR	2 cut(s)	1196, 2380
PpuMI	RGGWCCY	3 cut(s)	273, 592, 1188
PshAI	GACNNNNGTC	1 cut(s)	693
PsiI	TTATAA	1 cut(s)	555
Psp5II	RGGWCCY	3 cut(s)	273, 592, 1188
Psp6I	CCWGG	4 cut(s)	18, 199, 496, 595
PspEI	GGTNACC	1 cut(s)	2408
PspFI	CCCAGC	2 cut(s)	773, 1026
PspGI	CCWGG	4 cut(s)	18, 199, 496, 595
PspN4I	GGNNCC	8 cut(s)	88, 112, 454, 593, 594, 1473, 2457, 2578
PspPI	GGNCC	8 cut(s)	41, 273, 592, 751, 1188, 1295, 2438, 2456
PspPPI	RGGWCCY	3 cut(s)	273, 592, 1188
PsuI	RGATCY	2 cut(s)	574, 2135
PsyI	GACNNNGTC	1 cut(s)	859
PvuII	CAGCTG	1 cut(s)	1301
RsaI	GTAC	7 cut(s)	292, 431, 601, 1198, 1688, 1955, 2055
RsaNI	GTAC	7 cut(s)	291, 430, 600, 1197, 1687, 1954, 2054
RseI	CAYNNNNRTG	6 cut(s)	321, 375, 948, 1209, 1826, 2525
SaqAI	TTAA	5 cut(s)	699, 915, 924, 975, 1107
SatI	GCNGC	8 cut(s)	1262, 1302, 1331, 1632, 1656, 1883, 2019, 2037
Sau96I	GGNCC	8 cut(s)	41, 273, 592, 751, 1188, 1295, 2438, 2456
ScaI	AGTACT	3 cut(s)	1688, 1955, 2055
SchI	GAGTC	6 cut(s)	974, 1935, 2332, 2380, 2518, 2542
ScrFI	CCNGG	5 cut(s)	20, 201, 498, 597, 2466
SduI	GDGCHC	3 cut(s)	939, 1206, 2450
SfaNI	GCATC	8 cut(s)	200, 447, 637, 727, 949, 1084, 1411, 2005
SfoI	GGCGCC	1 cut(s)	2578
SinI	GGWCC	5 cut(s)	273, 592, 1188, 1295, 2438
SmiMI	CAYNNNNRTG	6 cut(s)	321, 375, 948, 1209, 1826, 2525
SmlI	CTYRAG	5 cut(s)	54, 698, 1480, 2233, 2417
SmoI	CTYRAG	5 cut(s)	54, 698, 1480, 2233, 2417
SnaBI	TACGTA	1 cut(s)	1196
SphI	GCATGC	1 cut(s)	185
SsiI	CCGC	6 cut(s)	531, 561, 624, 693, 754, 1655
SspDI	GGCGCC	1 cut(s)	2576
SspI	AATATT	1 cut(s)	2502
SspMI	CTAG	1 cut(s)	116
StyD4I	CCNGG	5 cut(s)	18, 199, 496, 595, 2464
StyI	CCWWGG	3 cut(s)	1256, 1265, 2160
TaaI	ACNGT	8 cut(s)	374, 426, 1078, 1201, 1283, 1958, 2274, 2452
TaiI	ACGT	6 cut(s)	970, 1159, 1198, 2127, 2382, 2490
TaqI	TCGA	4 cut(s)	964, 1009, 1235, 1944
TatI	WGTACW	4 cut(s)	429, 1686, 1953, 2053
TauI	GCSGC	1 cut(s)	1658
TfiI	GAWTC	3 cut(s)	23, 411, 1532
Tru1I	TTAA	5 cut(s)	699, 915, 924, 975, 1107
Tru9I	TTAA	5 cut(s)	699, 915, 924, 975, 1107
TscAI	CASTG	7 cut(s)	379, 484, 789, 1206, 1548, 1570, 2277
TseFI	GTSAC	4 cut(s)	780, 861, 2268, 2408
TseI	GCWGC	7 cut(s)	1261, 1301, 1330, 1631, 1882, 2018, 2036
Tsp45I	GTSAC	4 cut(s)	780, 861, 2268, 2408
TspDTI	ATGAA	7 cut(s)	813, 969, 1598, 1923, 2133, 2417, 2543
TspGWI	ACGGA	3 cut(s)	1511, 1640, 2259
TspRI	CASTG	7 cut(s)	379, 484, 789, 1206, 1548, 1570, 2277
Tth111I	GACNNNGTC	1 cut(s)	859
Van91I	CCANNNNNTGG	1 cut(s)	2489
Vha464I	CTTAAG	1 cut(s)	698
VneI	GTGCAC	1 cut(s)	1202
VpaK11BI	GGWCC	5 cut(s)	273, 592, 1188, 1295, 2438
XapI	RAATTY	4 cut(s)	1443, 1452, 1869, 1975
XceI	RCATGY	3 cut(s)	176, 185, 1631
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1263, 1691
XmiI	GTMKAC	2 cut(s)	527, 1624
XmnI	GAANNNNTTC	3 cut(s)	69, 144, 2334
XspI	CTAG	1 cut(s)	116
ZrmI	AGTACT	3 cut(s)	1688, 1955, 2055
Zsp2I	ATGCAT	2 cut(s)	945, 1426

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.