MD02G1294500.v1.1

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003606
Physical Location & Seq
Forward (+)
34943352 .. 34944584
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1294500.v1.1.491

Sequence Viewer

Length: 678 bp
ATGAAGACTGAACCTCTCCAGCTTAATGACATTGCTGAAGATGGTAACCTGTCCAATGGGAAGTACAAGAAGCCTATAACTTCAAGTTCCAATGAATCTGAGGATCAGAGTGATTTAAGGCTGAAAAAATCAGGTATCTCCAATAGCAACACAGGACAACCTGACAATGTGGAAGTGAAAAACAATTCAGAGCCAGAAGCCACAAAAAAATTCAACACGGCCAATAATAGCATTAGCCCTGGGATGAAGAGAAAAATTTCCGAGGCAGCAAGCAACTTCGACCAAGAGCTTCCCAACACCTACAACTACTGGAAAAGAATCAAATGCAAGAGACTTAGCATGGATGAGGAGGAGCAAAGGAAGTTCAATCCAAGAGAACCAAATTACCTACCTTTGGTTCCTGACCCCGAAGCAGAAAAGGTTGACCTCAGGCATCAGATTATTGATGAAAAGAAAAATGCAGATGAATGGATGCTTGACTTTGCACTCCAGCAGGCTGTCACGAAACTTGCTCCAGCAAGGAAGAAGAAGGTTGCACTGCTTGTTGCAGCTTTTGAAACGGTCATGCCAGCTCCCAAAGTGGAAAAACATCTCAGGCATACCTCCGCAGCCTTCAGTCATGCAAGACCTATGCAAGCTTGTAGTGAAAAGTCTGCAATATTGATATTAAGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

226

Amino Acids

25.44

Weight (kDa)

8.68

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 100 - 190 9.3e-31 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 606
AclWI GGATC 1 cut(s) 111
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 2 cut(s) 209, 255
AcuI CTGAAG 2 cut(s) 57, 598
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 1 cut(s) 394
AgsI TTSAA 4 cut(s) 84, 214, 367, 557
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 5 cut(s) 22, 289, 551, 572, 638
AluI AGCT 5 cut(s) 22, 289, 551, 572, 638
Alw26I GTCTC 1 cut(s) 325
AlwI GGATC 1 cut(s) 111
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 3 cut(s) 266, 548, 608
ApoI RAATTY 2 cut(s) 209, 255
Asp700I GAANNNNTTC 1 cut(s) 256
AxyI CCTNAGG 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 3 cut(s) 278, 560, 620
BccI CCATC 1 cut(s) 35
BceAI ACGGC 1 cut(s) 234
BciT130I CCWGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 325
BisI GCNGC 3 cut(s) 267, 549, 609
BlsI GCNGC 3 cut(s) 268, 550, 610
Bme1390I CCNGG 1 cut(s) 240
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 1 cut(s) 240
BmsI GCATC 2 cut(s) 442, 462
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 2 cut(s) 473, 498
BsaJI CCNNGG 3 cut(s) 238, 239, 261
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse1I ACTGG 1 cut(s) 314
Bse21I CCTNAGG 1 cut(s) 428
Bse3DI GCAATG 1 cut(s) 30
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 3 cut(s) 238, 239, 261
BseGI GGATG 3 cut(s) 249, 349, 477
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 3 cut(s) 90, 442, 607
BseNI ACTGG 1 cut(s) 314
BseRI GAGGAG 2 cut(s) 362, 365
BseXI GCAGC 3 cut(s) 278, 560, 620
BshFI GGCC 1 cut(s) 221
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 1 cut(s) 325
BsnI GGCC 1 cut(s) 221
Bsp143I GATC 1 cut(s) 103
BspACI CCGC 1 cut(s) 606
BspANI GGCC 1 cut(s) 221
BspCNI CTCAG 3 cut(s) 91, 441, 606
BspLI GGNNCC 1 cut(s) 399
BspPI GGATC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 30
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 3 cut(s) 238, 239, 261
BssMI GATC 1 cut(s) 103
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 562
Bst6I CTCTTC 1 cut(s) 242
BstC8I GCNNGC 4 cut(s) 271, 495, 570, 636
BstDEI CTNAG 4 cut(s) 99, 335, 428, 593
BstEII GGTNACC 1 cut(s) 44
BstF5I GGATG 3 cut(s) 249, 349, 477
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 1 cut(s) 325
BstMBI GATC 1 cut(s) 103
BstNI CCWGG 1 cut(s) 240
BstPI GGTNACC 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 238
BstV1I GCAGC 3 cut(s) 278, 560, 620
BstV2I GAAGAC 1 cut(s) 11
Bsu36I CCTNAGG 1 cut(s) 428
BsuRI GGCC 1 cut(s) 221
BtsCI GGATG 3 cut(s) 249, 349, 477
BtsI GCAGTG 1 cut(s) 536
BtsIMutI CAGTG 1 cut(s) 536
Cac8I GCNNGC 4 cut(s) 271, 495, 570, 636
Csp6I GTAC 1 cut(s) 64
CviAII CATG 3 cut(s) 340, 565, 620
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 4 cut(s) 99, 335, 428, 593
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
Eco57I CTGAAG 2 cut(s) 57, 598
Eco81I CCTNAGG 1 cut(s) 428
Eco91I GGTNACC 1 cut(s) 44
EcoO65I GGTNACC 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 238
FaeI CATG 3 cut(s) 343, 568, 623
FaiI YATR 6 cut(s) 77, 341, 566, 600, 621, 632
FatI CATG 3 cut(s) 339, 564, 619
Fnu4HI GCNGC 3 cut(s) 267, 549, 609
FokI GGATG 3 cut(s) 256, 356, 484
Fsp4HI GCNGC 3 cut(s) 267, 549, 609
GluI GCNGC 3 cut(s) 267, 549, 609
GsuI CTGGAG 2 cut(s) 473, 498
HaeIII GGCC 1 cut(s) 221
Hin1II CATG 3 cut(s) 343, 568, 623
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 2 cut(s) 95, 318
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 5 cut(s) 100, 108, 190, 262, 438
Hpy188III TCNNGA 2 cut(s) 401, 502
Hpy8I GTNNAC 1 cut(s) 424
HpyAV CCTTC 2 cut(s) 523, 622
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4V TGCA 8 cut(s) 327, 461, 485, 536, 548, 623, 634, 656
HpyF3I CTNAG 4 cut(s) 99, 335, 428, 593
Hsp92II CATG 3 cut(s) 343, 568, 623
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 3 cut(s) 352, 517, 577
Lsp1109I GCAGC 3 cut(s) 278, 560, 620
LweI GCATC 2 cut(s) 442, 462
MaeIII GTNAC 3 cut(s) 44, 499, 671
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 5 cut(s) 16, 50, 259, 535, 538
MluCI AATT 4 cut(s) 184, 209, 255, 382
MnlI CCTC 7 cut(s) 24, 94, 256, 340, 343, 437, 613
MroXI GAANNNNTTC 1 cut(s) 256
MseI TTAA 3 cut(s) 24, 116, 668
MspR9I CCNGG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 240
NdeII GATC 1 cut(s) 103
NlaIII CATG 3 cut(s) 343, 568, 623
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 1 cut(s) 499
PasI CCCWGGG 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 256
PfeI GAWTC 2 cut(s) 95, 318
PkrI GCNGC 3 cut(s) 268, 550, 610
Psp6I CCWGG 1 cut(s) 238
PspEI GGTNACC 1 cut(s) 44
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 1 cut(s) 399
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
SaqAI TTAA 3 cut(s) 24, 116, 668
SatI GCNGC 3 cut(s) 267, 549, 609
Sau3AI GATC 1 cut(s) 103
ScrFI CCNGG 1 cut(s) 240
SfaNI GCATC 2 cut(s) 442, 462
Sse9I AATT 4 cut(s) 184, 209, 255, 382
SsiI CCGC 1 cut(s) 606
SspI AATATT 1 cut(s) 660
StyD4I CCNGG 1 cut(s) 238
TaaI ACNGT 1 cut(s) 562
TaqI TCGA 1 cut(s) 279
TasI AATT 4 cut(s) 184, 209, 255, 382
TatI WGTACW 1 cut(s) 63
TfiI GAWTC 2 cut(s) 95, 318
Tru1I TTAA 3 cut(s) 24, 116, 668
Tru9I TTAA 3 cut(s) 24, 116, 668
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 3 cut(s) 266, 548, 608
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 5 cut(s) 17, 108, 260, 462, 480
TspRI CASTG 1 cut(s) 543
XapI RAATTY 2 cut(s) 209, 255
XmnI GAANNNNTTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.