RLG00000002328

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003606
Physical Location & Seq
Reverse (-)
32236484 .. 32237254
771 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002328

Sequence Viewer

Length: 771 bp
ATGGTTGTACATGATGAAGATGATAGTAAGGTTGGTGATATTGCAGACACTGCTGAAGATTGTAATTCAGGCCAAGGTATTGTTGATGAAAACACTTTTGTTGAGAGTAAAGATAACCTGGCCAATGGGGAGTACACGGATAATGCTAAAGTTGAATATCATAGCTTCACAGAGAAGGATCAACGTGAATTTAAGAAGGAAAAGATCTCAAGTTCCAGTGAAGGTGAGGATCAGAGTGATCTAAAGCTGAAGCACACAGATGTGGCAGAAAACAGTGGCGGAGACATTGATAGAATGGAAGTGGACAACACTTCAGATCCAGAAGCAGCAGAAACTTTCCTCATGGCCAATAGTAGCATCAGTCCAGGGATGAAGAGAAAGTTTTCCCATCAAGAAAGCAATTCGGACGAAGAACTGCAATACACAAACCGGAAATGGAAAATCAATTGCAAGAGAAGTATCAAGGATGAGGAAAAACAATGGAAATATAATCAAAGAGAACCCAATTACCTGCCGTTGGTTCGTGGCCCTGAAGCAAAGAAGTTTGACTTGAGGCATCAGATGATAGATGAAAAGAAACATGCAGAGGAATGGATGCTCGACTATGCAATCCAACAAGCTGTAACCAAACTTGTTCCAGCTAGGAAGAAGAAGGTGGAATTGCTTGTTGAAGCTTTTGAGAAAGTCATGCCAGTGCCGCAATATGAACCCTGTTTGAAGCATTCTTCAGCAGCCTTCAGTCATGCAAGACCTATGCAAGCTTGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

257

Amino Acids

29.34

Weight (kDa)

5.11

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 131 - 231 3.7e-28 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 519
AciI CCGC 2 cut(s) 279, 698
AclWI GGATC 3 cut(s) 186, 237, 311
AcoI YGGCCR 2 cut(s) 120, 345
AcsI RAATTY 1 cut(s) 188
AcuI CTGAAG 6 cut(s) 75, 269, 297, 552, 711, 721
AfaI GTAC 2 cut(s) 9, 134
AfiI CCNNNNNNNGG 1 cut(s) 517
AgsI TTSAA 3 cut(s) 155, 671, 718
AjnI CCWGG 2 cut(s) 117, 364
AleI CACNNNNGTG 1 cut(s) 260
AluBI AGCT 7 cut(s) 165, 247, 620, 641, 674, 761, 768
AluI AGCT 7 cut(s) 165, 247, 620, 641, 674, 761, 768
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 3 cut(s) 186, 237, 311
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 4 cut(s) 70, 120, 345, 526
ApeKI GCWGC 2 cut(s) 326, 731
ApoI RAATTY 1 cut(s) 188
Asp700I GAANNNNTTC 1 cut(s) 382
AspS9I GGNCC 1 cut(s) 527
AsuHPI GGTGA 2 cut(s) 47, 236
BalI TGGCCA 2 cut(s) 122, 347
BbvI GCAGC 2 cut(s) 338, 743
BccI CCATC 1 cut(s) 396
BceAI ACGGC 1 cut(s) 499
BciT130I CCWGG 2 cut(s) 119, 366
BcoDI GTCTC 1 cut(s) 276
BfaI CTAG 1 cut(s) 642
BfuAI ACCTGC 1 cut(s) 519
BglII AGATCT 1 cut(s) 204
BisI GCNGC 3 cut(s) 327, 698, 732
BlsI GCNGC 3 cut(s) 328, 699, 733
Bme1390I CCNGG 2 cut(s) 119, 366
BmgT120I GGNCC 1 cut(s) 527
BmrFI CCNGG 2 cut(s) 119, 366
BmsI GCATC 3 cut(s) 366, 565, 585
BpuEI CTTGAG 2 cut(s) 193, 571
BsaJI CCNNGG 2 cut(s) 73, 365
BsaWI WCCGGW 1 cut(s) 429
Bsc4I CCNNNNNNNGG 1 cut(s) 517
Bse1I ACTGG 2 cut(s) 216, 692
BseBI CCWGG 2 cut(s) 119, 366
BseDI CCNNGG 2 cut(s) 73, 365
BseGI GGATG 3 cut(s) 375, 472, 600
BseLI CCNNNNNNNGG 1 cut(s) 517
BseNI ACTGG 2 cut(s) 216, 692
BseXI GCAGC 2 cut(s) 338, 743
BshFI GGCC 4 cut(s) 72, 122, 347, 528
BsiSI CCGG 1 cut(s) 430
BslI CCNNNNNNNGG 1 cut(s) 517
BsmAI GTCTC 1 cut(s) 276
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 4 cut(s) 72, 122, 347, 528
Bsp1407I TGTACA 1 cut(s) 7
Bsp143I GATC 5 cut(s) 178, 204, 229, 238, 316
BspACI CCGC 2 cut(s) 279, 698
BspANI GGCC 4 cut(s) 72, 122, 347, 528
BspMI ACCTGC 1 cut(s) 519
BspPI GGATC 3 cut(s) 186, 237, 311
BsrGI TGTACA 1 cut(s) 7
BsrI ACTGG 2 cut(s) 216, 692
BssECI CCNNGG 2 cut(s) 73, 365
BssMI GATC 5 cut(s) 178, 204, 229, 238, 316
BssT1I CCWWGG 1 cut(s) 73
Bst2UI CCWGG 2 cut(s) 119, 366
Bst4CI ACNGT 1 cut(s) 275
Bst6I CTCTTC 1 cut(s) 368
BstAPI GCANNNNNTGC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 7
BstC8I GCNNGC 1 cut(s) 759
BstF5I GGATG 3 cut(s) 375, 472, 600
BstKTI GATC 5 cut(s) 181, 207, 232, 241, 319
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 5 cut(s) 178, 204, 229, 238, 316
BstMWI GCNNNNNNNGC 2 cut(s) 50, 697
BstNI CCWGG 2 cut(s) 119, 366
BstNSI RCATGY 1 cut(s) 584
BstSCI CCNGG 2 cut(s) 117, 364
BstV1I GCAGC 2 cut(s) 338, 743
BstX2I RGATCY 2 cut(s) 204, 316
BstYI RGATCY 2 cut(s) 204, 316
BsuRI GGCC 4 cut(s) 72, 122, 347, 528
BtsCI GGATG 3 cut(s) 375, 472, 600
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 4 cut(s) 48, 223, 280, 699
BveI ACCTGC 1 cut(s) 519
Cac8I GCNNGC 1 cut(s) 759
CaiI CAGNNNCTG 1 cut(s) 50
Cfr13I GGNCC 1 cut(s) 527
Csp6I GTAC 2 cut(s) 8, 133
CviAII CATG 5 cut(s) 11, 343, 581, 688, 743
CviQI GTAC 2 cut(s) 8, 133
DpnI GATC 5 cut(s) 180, 206, 231, 240, 318
DpnII GATC 5 cut(s) 178, 204, 229, 238, 316
EaeI YGGCCR 2 cut(s) 120, 345
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
EciI GGCGGA 1 cut(s) 294
Eco130I CCWWGG 1 cut(s) 73
Eco57I CTGAAG 6 cut(s) 75, 269, 297, 552, 711, 721
EcoRII CCWGG 2 cut(s) 117, 364
EcoT14I CCWWGG 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 5 cut(s) 14, 346, 584, 691, 746
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FatI CATG 5 cut(s) 10, 342, 580, 687, 742
Fnu4HI GCNGC 3 cut(s) 327, 698, 732
FokI GGATG 3 cut(s) 382, 479, 607
Fsp4HI GCNGC 3 cut(s) 327, 698, 732
FspBI CTAG 1 cut(s) 642
GluI GCNGC 3 cut(s) 327, 698, 732
HaeIII GGCC 4 cut(s) 72, 122, 347, 528
HapII CCGG 1 cut(s) 430
Hin1II CATG 5 cut(s) 14, 346, 584, 691, 746
HindIII AAGCTT 2 cut(s) 672, 759
HpaII CCGG 1 cut(s) 430
HphI GGTGA 2 cut(s) 47, 236
Hpy166II GTNNAC 2 cut(s) 135, 304
Hpy188I TCNGA 4 cut(s) 234, 316, 406, 561
Hpy188III TCNNGA 2 cut(s) 320, 392
Hpy8I GTNNAC 2 cut(s) 135, 304
HpyAV CCTTC 5 cut(s) 169, 190, 215, 646, 745
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 7 cut(s) 44, 418, 450, 584, 608, 746, 757
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 697
HpySE526I ACGT 1 cut(s) 184
Hsp92II CATG 5 cut(s) 14, 346, 584, 691, 746
Kzo9I GATC 5 cut(s) 178, 204, 229, 238, 316
Lsp1109I GCAGC 2 cut(s) 338, 743
LweI GCATC 3 cut(s) 366, 565, 585
MaeI CTAG 1 cut(s) 642
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 1 cut(s) 622
MalI GATC 5 cut(s) 180, 206, 231, 240, 318
MboI GATC 5 cut(s) 178, 204, 229, 238, 316
MboII GAAGA 7 cut(s) 29, 68, 385, 422, 658, 661, 717
MfeI CAATTG 1 cut(s) 445
MflI RGATCY 2 cut(s) 204, 316
MlsI TGGCCA 2 cut(s) 122, 347
MluCI AATT 6 cut(s) 64, 188, 400, 445, 505, 659
MluNI TGGCCA 2 cut(s) 122, 347
MmeI TCCRAC 1 cut(s) 637
MnlI CCTC 5 cut(s) 220, 350, 463, 546, 580
Mox20I TGGCCA 2 cut(s) 122, 347
MroXI GAANNNNTTC 1 cut(s) 382
MscI TGGCCA 2 cut(s) 122, 347
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 3 cut(s) 258, 260, 692
Msp20I TGGCCA 2 cut(s) 122, 347
MspI CCGG 1 cut(s) 430
MspR9I CCNGG 2 cut(s) 119, 366
MunI CAATTG 1 cut(s) 445
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 2 cut(s) 119, 366
MwoI GCNNNNNNNGC 2 cut(s) 50, 697
NdeII GATC 5 cut(s) 178, 204, 229, 238, 316
NlaIII CATG 5 cut(s) 14, 346, 584, 691, 746
NspI RCATGY 1 cut(s) 584
OliI CACNNNNGTG 1 cut(s) 260
PctI GAATGC 1 cut(s) 721
PdmI GAANNNNTTC 1 cut(s) 382
PkrI GCNGC 3 cut(s) 328, 699, 733
Psp6I CCWGG 2 cut(s) 117, 364
PspGI CCWGG 2 cut(s) 117, 364
PspPI GGNCC 1 cut(s) 527
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 2 cut(s) 204, 316
RsaI GTAC 2 cut(s) 9, 134
RsaNI GTAC 2 cut(s) 8, 133
RseI CAYNNNNRTG 3 cut(s) 258, 260, 692
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 3 cut(s) 327, 698, 732
Sau3AI GATC 5 cut(s) 178, 204, 229, 238, 316
Sau96I GGNCC 1 cut(s) 527
ScrFI CCNGG 2 cut(s) 119, 366
SfaNI GCATC 3 cut(s) 366, 565, 585
SmiMI CAYNNNNRTG 3 cut(s) 258, 260, 692
SmlI CTYRAG 2 cut(s) 208, 550
SmoI CTYRAG 2 cut(s) 208, 550
Sse9I AATT 6 cut(s) 64, 188, 400, 445, 505, 659
SsiI CCGC 2 cut(s) 279, 698
SspMI CTAG 1 cut(s) 642
StyD4I CCNGG 2 cut(s) 117, 364
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 1 cut(s) 275
TaiI ACGT 1 cut(s) 187
TaqI TCGA 1 cut(s) 600
TasI AATT 6 cut(s) 64, 188, 400, 445, 505, 659
TatI WGTACW 2 cut(s) 7, 132
TauI GCSGC 1 cut(s) 700
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 4 cut(s) 55, 223, 280, 699
TseI GCWGC 2 cut(s) 326, 731
TspDTI ATGAA 5 cut(s) 30, 102, 386, 585, 720
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 4 cut(s) 55, 223, 280, 699
XapI RAATTY 1 cut(s) 188
XceI RCATGY 1 cut(s) 584
XmnI GAANNNNTTC 1 cut(s) 382
XspI CTAG 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.