pycom02g24790

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003606
Physical Location & Seq
Forward (+)
22893699 .. 22896652
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g24790.1

Sequence Viewer

Length: 2820 bp
ATGGTCCAAAGAAAGGTGCCAAACAAGCTTGGAATCCAAGCTGATCATGATAAATTCGAGATGCGGTTTTCGAACCTGAAGACTTCTTCTCAGTTCCAAGATGGGAAACACAGAGGAGCTGATTTGAAGAAGAAGATGAAGAAATCTAGATCAATCAAGCTTTCAGATGTTGAGAGCTTGAGATCATCACCTTTGAGGAAGGACTCATCTCAGCCAGGAAAGCCGCCACCACCGGCTCTCAATGTTCCAAACACTGCGGCTTCCCCTCAGAAGCAGCCCCTGGCCAAAACAACTTATGGCTCGCCAAACTACATGAAGCCCACCAGCTGTTCTCATGCAAGGAAGGAGCAGTCACAGGTTAGTCTTCGAAGTTCTCCGCCAATATTTTCGGATAGTAAGAACCAGAACCGAAAAAATTCGGGCAGTTCAAAGCTTAGCTCTGCTTCTAGTAAACCTGAAAGAAGTTTAGCAAGGACATCTAGTTTGAAGCTTGTGAGGACTTTAATAAGGTCCCCTAGTTTTAAGTCAGCAAGAGCTCCGGCGAGAAAATCTTCCAGGGTTGCTCTTTGTGCAGATGTTAATGTTCAGAGAGAAACTTGTTCTTCAACACTGAAGGATACAAAGTTTCCGGATTATCTGATGATTAGTCCTGGGGGAACTGAAGCTGAGGGAACTTCAGTCATGAAGGTTTGCCCGTATACGTACTGTTCCCTTAATGGACATCGTCATTCACCTGTGCCGCCATTGAAGAGTTTCTTGTCTGCAAGAAGACGTTCATTGAAGACCCAGAAGATGATGAAATTGCAAGCTCTAAGTCCGCGTGGAGCAAAGCGATGTAATGATGGAGTAAAAGAAATTGATTTCCAGCAGATGTTTGATGAGAATGACAAAACAGCTGAGAAAGAAGCTGATTTGGATTTCTTCGTTGAAATCTATGCTACAAACAAGGAAGATGACACTGAGGCAATTGGAAGGAAAGCCGGAGCTGATTCTGTAGGAGAACAAGATGGTTACGAGGGTCCTGTGTTTCCAAATGCTGCAAGTGATGAAACAGAAACAGTAGATTCTGCCAACAACCTACTTGTGGAGAACCTGTCTGATAGATCACTGCATTCTGAGAGTGAATCTGAGGCAGAAAACTATGGCGAATTTCCTGAAGATCGAAAAGAAGATGCAAATGAAGACTACGGATCACTGTCCGATCAAGAGGAAAATTCCACTGGAAGCTGCTCAAACGAGAGCAACTCTGAAGACCTTTCAAGCACTGAAATGGATTGTTCCAGTTCTGAAACTACTGACATGGAGTGGGAGGAAGGGCAATTTTCCACTGCAGTGCTTGATGGTTATGAATCTGGTCCAAATGCGGGGTGTTCAATAACAATTCAAGATGCTGATATGCATGAAGAATCTCTGATCAAGTCTGATGCAATGAATGGTGACTACGATAATTTGGTTCAGGATTTTTATGTAGTACTGCAGAATGATGGTGATGTCAGTGAACAGGATGGCATAAAAAAGAATTTTGAGATTCTAGAATCTAGACACGTGCACGAAAGAATGAGTTATGACCAACTTTCCTACAGCGATGATGCATTCGAAGAAGGCAGTGAACTATCAGAGACAGACTGTGTAGAGATATCTTCATCTTCAATGGAGGAGCCAAATGAGGAACTACCAACAACTGGCAAGGAACTTCAAGAGCCGAATGGAGTTGCTGAAGCAGAGGATCATGAAATCGATTCTCAGCTTGGAGATGTTGAAAGCAACTGCAGCATTGTGGAGACAGGCAAAGCTTCAGATAACCAACCAAAGAATGCATTTCATGAGGATGAAACAAGTACAGTGACAGGAGACCGAATTTCTATTCCCTCTCAGGATATGAGCGAAACAGATGATGCTGAAGCAAATGAAGGATGCAACGGGAGCCCTGATAAAGGGAATTCAGAAACAGATCAAAGCGTTCCAAGTGGTGGCATTGGCTCAGAACCCAAACTCCCTACAGTGGTTTCTGAAAATAAAATGGAAGCAGTAGAGCAAATTGGTGATGTGAAGCCATCACCGGAGATTCAGCTTTCTGATTCGGTGCACGATGCATCTGAAGCTGATGAAGATGAGGTCAAGGTTGACGATGATGACAATTACAAGAAGACTGAACCTCTCCAGGTTAATGACATTGCTGAAGATGGTAACCTGTCCAACGGGAAGTACAAGAAGCCTATAACTTCAAGTTCCAATGAATCTGAGGATCAGAGTGATTTAAGGCTGAAAAAATCAGGTATCTCCAATAGCAACACAGGACAACCTCATACTGTGGAAGTGAAATACAATTCAGAGCCAGAAGCCACAAAAAATTTCAACACGGCCAATAATAGCATTAGCCCTGGGATGAAGAGAAAATTTTCCGAGGCAGCAAGCAACTTCGAACAAGAACTTCCCAACACCTACAACCACTGGAAAAGAATCAAATGCAAGAGACTTAGCATGGATGAGGAGGAGCAAAGGAAGTTCAATCCAAGAGAACCGAATTACCTACCTTTGGTTCCTGACCCTGAAGCAGAAAAGGTTGACCTCAGGCATCAGATTATTGATGAAAAGAAAAATGCAGATGAATGGATGCTTGACTTTGCACTCCAGCAGGCTGTCACAAAACTTGCTCCAGCAAGGAAGAAGAAGGTTGCACTGCTTGTTGCAGCTTTCGAAGCGGTCATGCCAGCGCCCAAAGTGGAAAAACATCTTAAGCATCCCTCAGCAGCCTTCAGTCACGCAAGACCTATGCAAGCTTGTAGTGAAAAGACTGCAATATCGATAATAAGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

940

Amino Acids

103.65

Weight (kDa)

4.83

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 801 - 904 4.1e-29 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 16
AccB7I CCANNNNNTGG 2 cut(s) 1682, 1970
AccI GTMKAC 1 cut(s) 698
AccII CGCG 1 cut(s) 820
AccIII TCCGGA 1 cut(s) 628
AciI CCGC 8 cut(s) 64, 224, 257, 377, 740, 818, 1364, 2702
AclWI GGATC 3 cut(s) 1198, 1734, 2253
AcoI YGGCCR 2 cut(s) 282, 2361
AcsI RAATTY 9 cut(s) 53, 415, 1148, 1213, 1519, 1857, 1939, 2350, 2396
AcvI CACGTG 1 cut(s) 1546
AfaI GTAC 4 cut(s) 704, 1473, 1840, 2207
AfiI CCNNNNNNNGG 8 cut(s) 13, 1084, 1364, 1682, 1934, 1970, 2002, 2536
AflII CTTAAG 1 cut(s) 2735
AflIII ACRYGT 1 cut(s) 1543
AjnI CCWGG 6 cut(s) 214, 279, 554, 649, 2160, 2380
AleI CACNNNNGTG 1 cut(s) 1331
AloI GAACNNNNNNTCC 2 cut(s) 1977, 2009
Alw21I GWGCWC 3 cut(s) 538, 1551, 2088
Alw26I GTCTC 4 cut(s) 1613, 1775, 1845, 2467
Alw44I GTGCAC 2 cut(s) 1547, 2084
AlwI GGATC 3 cut(s) 1198, 1734, 2253
AlwNI CAGNNNCTG 1 cut(s) 280
Aor13HI TCCGGA 1 cut(s) 628
AoxI GGCC 2 cut(s) 282, 2361
ApaLI GTGCAC 2 cut(s) 1547, 2084
ApeKI GCWGC 7 cut(s) 274, 1037, 1227, 1770, 2408, 2690, 2750
ApoI RAATTY 9 cut(s) 53, 415, 1148, 1213, 1519, 1857, 1939, 2350, 2396
ArsI GACNNNNNNTTYG 2 cut(s) 466, 498
Asp700I GAANNNNTTC 5 cut(s) 415, 550, 752, 772, 2398
AspLEI GCGC 1 cut(s) 2716
AspS9I GGNCC 4 cut(s) 4, 510, 1019, 1355
AsuHPI GGTGA 6 cut(s) 180, 723, 1448, 1499, 2049, 2054
AsuII TTCGAA 5 cut(s) 71, 367, 1596, 2421, 2697
AvaII GGWCC 4 cut(s) 4, 510, 1019, 1355
AxyI CCTNAGG 1 cut(s) 2570
BaeGI GKGCMC 2 cut(s) 1551, 2088
BalI TGGCCA 1 cut(s) 284
BanI GGYRCC 1 cut(s) 16
BanII GRGCYC 2 cut(s) 538, 1928
BbrPI CACGTG 1 cut(s) 1546
BbsI GAAGAC 7 cut(s) 86, 356, 775, 788, 1188, 1257, 2153
Bbv12I GWGCWC 3 cut(s) 538, 1551, 2088
BbvCI CCTCAGC 2 cut(s) 666, 2746
BbvI GCAGC 7 cut(s) 286, 1024, 1214, 1782, 2420, 2702, 2762
BccI CCATC 8 cut(s) 95, 836, 1001, 1334, 1478, 1499, 2062, 2177
BceAI ACGGC 1 cut(s) 2376
BciT130I CCWGG 6 cut(s) 216, 281, 556, 651, 2162, 2382
BciVI GTATCC 1 cut(s) 610
BclI TGATCA 2 cut(s) 43, 1413
BcoDI GTCTC 4 cut(s) 1613, 1775, 1845, 2467
BfaI CTAG 6 cut(s) 147, 447, 480, 516, 1532, 1539
BfmI CTRYAG 6 cut(s) 993, 1329, 1475, 1579, 1768, 1998
BfoI RGCGCY 1 cut(s) 2717
BfrI CTTAAG 1 cut(s) 2735
BfuI GTATCC 1 cut(s) 610
BlpI GCTNAGC 1 cut(s) 434
BmcAI AGTACT 1 cut(s) 1473
Bme1390I CCNGG 6 cut(s) 216, 281, 556, 651, 2162, 2382
Bme18I GGWCC 4 cut(s) 4, 510, 1019, 1355
BmgT120I GGNCC 4 cut(s) 4, 510, 1019, 1355
BmiI GGNNCC 6 cut(s) 18, 512, 1020, 1659, 1925, 2541
BmrFI CCNGG 6 cut(s) 216, 281, 556, 651, 2162, 2382
BpiI GAAGAC 7 cut(s) 86, 356, 775, 788, 1188, 1257, 2153
BplI GAGNNNNNCTC 2 cut(s) 1229, 1261
BpmI CTGGAG 3 cut(s) 2144, 2615, 2640
Bpu10I CCTNAGC 2 cut(s) 666, 2746
Bpu1102I GCTNAGC 1 cut(s) 434
Bpu14I TTCGAA 5 cut(s) 71, 367, 1596, 2421, 2697
BpuEI CTTGAG 1 cut(s) 199
Bsa29I ATCGAT 2 cut(s) 1737, 2804
BsaAI YACGTR 2 cut(s) 702, 1546
BsaI GGTCTC 1 cut(s) 1845
BsaJI CCNNGG 6 cut(s) 279, 555, 650, 2380, 2381, 2403
BsaWI WCCGGW 2 cut(s) 628, 2059
BsaXI ACNNNNNCTCC 4 cut(s) 1079, 1109, 1977, 2007
Bsc4I CCNNNNNNNGG 8 cut(s) 13, 1084, 1364, 1682, 1934, 1970, 2002, 2536
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 4 cut(s) 1225, 1281, 1687, 2456
Bse21I CCTNAGG 1 cut(s) 2570
Bse3DI GCAATG 2 cut(s) 1434, 2172
BseAI TCCGGA 1 cut(s) 628
BseBI CCWGG 6 cut(s) 216, 281, 556, 651, 2162, 2382
BseCI ATCGAT 2 cut(s) 1737, 2804
BseDI CCNNGG 6 cut(s) 279, 555, 650, 2380, 2381, 2403
BseGI GGATG 7 cut(s) 1510, 1834, 1919, 2391, 2491, 2619, 2740
BseLI CCNNNNNNNGG 8 cut(s) 13, 1084, 1364, 1682, 1934, 1970, 2002, 2536
BseMI GCAATG 2 cut(s) 1434, 2172
BseNI ACTGG 4 cut(s) 1225, 1281, 1687, 2456
BseRI GAGGAG 4 cut(s) 129, 1670, 2504, 2507
BseSI GKGCMC 2 cut(s) 1551, 2088
BseXI GCAGC 7 cut(s) 286, 1024, 1214, 1782, 2420, 2702, 2762
BsgI GTGCAG 1 cut(s) 591
Bsh1236I CGCG 1 cut(s) 820
BshFI GGCC 2 cut(s) 284, 2363
BshNI GGYRCC 1 cut(s) 16
BshVI ATCGAT 2 cut(s) 1737, 2804
BsiHKAI GWGCWC 3 cut(s) 538, 1551, 2088
BsiSI CCGG 5 cut(s) 233, 539, 629, 981, 2060
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 8 cut(s) 13, 1084, 1364, 1682, 1934, 1970, 2002, 2536
BsmAI GTCTC 4 cut(s) 1613, 1775, 1845, 2467
BsmFI GGGAC 1 cut(s) 496
BsmI GAATGC 3 cut(s) 1111, 1592, 1819
BsnI GGCC 2 cut(s) 284, 2363
Bso31I GGTCTC 1 cut(s) 1845
Bsp119I TTCGAA 5 cut(s) 71, 367, 1596, 2421, 2697
Bsp1286I GDGCHC 4 cut(s) 538, 1551, 1928, 2088
Bsp13I TCCGGA 1 cut(s) 628
Bsp1720I GCTNAGC 1 cut(s) 434
BspACI CCGC 8 cut(s) 64, 224, 257, 377, 740, 818, 1364, 2702
BspANI GGCC 2 cut(s) 284, 2363
BspDI ATCGAT 2 cut(s) 1737, 2804
BspEI TCCGGA 1 cut(s) 628
BspFNI CGCG 1 cut(s) 820
BspHI TCATGA 4 cut(s) 46, 681, 1729, 1822
BspLI GGNNCC 6 cut(s) 18, 512, 1020, 1659, 1925, 2541
BspMAI CTGCAG 3 cut(s) 1333, 1479, 1772
BspPI GGATC 3 cut(s) 1198, 1734, 2253
BspT104I TTCGAA 5 cut(s) 71, 367, 1596, 2421, 2697
BspT107I GGYRCC 1 cut(s) 16
BspTI CTTAAG 1 cut(s) 2735
BspTNI GGTCTC 1 cut(s) 1845
BsrDI GCAATG 2 cut(s) 1434, 2172
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 4 cut(s) 1225, 1281, 1687, 2456
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 6 cut(s) 279, 555, 650, 2380, 2381, 2403
BssNAI GTATAC 1 cut(s) 699
Bst1107I GTATAC 1 cut(s) 699
Bst2UI CCWGG 6 cut(s) 216, 281, 556, 651, 2162, 2382
Bst4CI ACNGT 7 cut(s) 707, 1060, 1197, 1628, 1843, 2002, 2311
Bst6I CTCTTC 2 cut(s) 743, 2384
BstAFI CTTAAG 1 cut(s) 2735
BstBAI YACGTR 2 cut(s) 702, 1546
BstBI TTCGAA 5 cut(s) 71, 367, 1596, 2421, 2697
BstC8I GCNNGC 6 cut(s) 302, 807, 2413, 2637, 2712, 2778
BstEII GGTNACC 1 cut(s) 2186
BstENI CCTNNNNNAGG 1 cut(s) 1932
BstF5I GGATG 7 cut(s) 1510, 1834, 1919, 2391, 2491, 2619, 2740
BstFNI CGCG 1 cut(s) 820
BstH2I RGCGCY 1 cut(s) 2717
BstHHI GCGC 1 cut(s) 2716
BstMAI GTCTC 4 cut(s) 1613, 1775, 1845, 2467
BstMWI GCNNNNNNNGC 7 cut(s) 25, 220, 569, 1770, 1923, 2099, 2699
BstNI CCWGG 6 cut(s) 216, 281, 556, 651, 2162, 2382
BstPI GGTNACC 1 cut(s) 2186
BstSCI CCNGG 6 cut(s) 214, 279, 554, 649, 2160, 2380
BstSFI CTRYAG 6 cut(s) 993, 1329, 1475, 1579, 1768, 1998
BstSLI GKGCMC 2 cut(s) 1551, 2088
BstSNI TACGTA 1 cut(s) 702
BstUI CGCG 1 cut(s) 820
BstV1I GCAGC 7 cut(s) 286, 1024, 1214, 1782, 2420, 2702, 2762
BstV2I GAAGAC 7 cut(s) 86, 356, 775, 788, 1188, 1257, 2153
BstZ17I GTATAC 1 cut(s) 699
Bsu15I ATCGAT 2 cut(s) 1737, 2804
Bsu36I CCTNAGG 1 cut(s) 2570
BsuI GTATCC 1 cut(s) 610
BsuRI GGCC 2 cut(s) 284, 2363
BsuTUI ATCGAT 2 cut(s) 1737, 2804
BtgZI GCGATG 2 cut(s) 847, 1599
BtsCI GGATG 7 cut(s) 1510, 1834, 1919, 2391, 2491, 2619, 2740
BtsI GCAGTG 6 cut(s) 252, 1106, 1326, 1338, 1612, 2678
Cac8I GCNNGC 6 cut(s) 302, 807, 2413, 2637, 2712, 2778
CaiI CAGNNNCTG 1 cut(s) 280
CciI TCATGA 4 cut(s) 46, 681, 1729, 1822
CfoI GCGC 1 cut(s) 2716
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 4 cut(s) 4, 510, 1019, 1355
ClaI ATCGAT 2 cut(s) 1737, 2804
Csp6I GTAC 4 cut(s) 703, 1472, 1839, 2206
CviQI GTAC 4 cut(s) 703, 1472, 1839, 2206
EaeI YGGCCR 2 cut(s) 282, 2361
Eam1104I CTCTTC 2 cut(s) 743, 2384
EarI CTCTTC 2 cut(s) 743, 2384
EciI GGCGGA 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 536
Eco105I TACGTA 1 cut(s) 702
Eco24I GRGCYC 2 cut(s) 538, 1928
Eco31I GGTCTC 1 cut(s) 1845
Eco32I GATATC 1 cut(s) 1638
Eco47I GGWCC 4 cut(s) 4, 510, 1019, 1355
Eco53kI GAGCTC 1 cut(s) 536
Eco72I CACGTG 1 cut(s) 1546
Eco81I CCTNAGG 1 cut(s) 2570
Eco91I GGTNACC 1 cut(s) 2186
EcoICRI GAGCTC 1 cut(s) 536
EcoNI CCTNNNNNAGG 1 cut(s) 1932
EcoO109I RGGNCCY 2 cut(s) 510, 1019
EcoO65I GGTNACC 1 cut(s) 2186
EcoRI GAATTC 1 cut(s) 1939
EcoRII CCWGG 6 cut(s) 214, 279, 554, 649, 2160, 2380
EcoRV GATATC 1 cut(s) 1638
EcoT22I ATGCAT 4 cut(s) 1401, 1594, 1819, 2095
EcoT38I GRGCYC 2 cut(s) 538, 1928
FalI AAGNNNNNCTT 2 cut(s) 740, 772
FaqI GGGAC 1 cut(s) 496
FauI CCCGC 1 cut(s) 1357
FbaI TGATCA 2 cut(s) 43, 1413
FblI GTMKAC 1 cut(s) 698
FokI GGATG 7 cut(s) 1517, 1841, 1926, 2398, 2498, 2626, 2727
FriOI GRGCYC 2 cut(s) 538, 1928
FspBI CTAG 6 cut(s) 147, 447, 480, 516, 1532, 1539
GlaI GCGC 1 cut(s) 2715
GsuI CTGGAG 3 cut(s) 2144, 2615, 2640
HaeII RGCGCY 1 cut(s) 2717
HaeIII GGCC 2 cut(s) 284, 2363
HapII CCGG 5 cut(s) 233, 539, 629, 981, 2060
HhaI GCGC 1 cut(s) 2716
Hin6I GCGC 1 cut(s) 2714
HinP1I GCGC 1 cut(s) 2714
HincII GTYRAC 2 cut(s) 2125, 2566
HindII GTYRAC 2 cut(s) 2125, 2566
HindIII AAGCTT 6 cut(s) 26, 158, 431, 488, 1791, 2778
HpaII CCGG 5 cut(s) 233, 539, 629, 981, 2060
HphI GGTGA 6 cut(s) 180, 723, 1448, 1499, 2049, 2054
Hpy166II GTNNAC 8 cut(s) 452, 699, 1499, 1549, 1610, 2086, 2125, 2566
Hpy8I GTNNAC 8 cut(s) 452, 699, 1499, 1549, 1610, 2086, 2125, 2566
HpyCH4III ACNGT 7 cut(s) 707, 1060, 1197, 1628, 1843, 2002, 2311
HpyCH4IV ACGT 3 cut(s) 701, 772, 1545
HpyF10VI GCNNNNNNNGC 7 cut(s) 25, 220, 569, 1770, 1923, 2099, 2699
HpySE526I ACGT 3 cut(s) 701, 772, 1545
HspAI GCGC 1 cut(s) 2714
Kpn2I TCCGGA 1 cut(s) 628
Ksp22I TGATCA 2 cut(s) 43, 1413
LmnI GCTCC 9 cut(s) 116, 346, 541, 824, 983, 1657, 1923, 2494, 2659
Lsp1109I GCAGC 7 cut(s) 286, 1024, 1214, 1782, 2420, 2702, 2762
MaeI CTAG 6 cut(s) 147, 447, 480, 516, 1532, 1539
MaeII ACGT 3 cut(s) 701, 772, 1545
MaeIII GTNAC 8 cut(s) 351, 1010, 1436, 1843, 2186, 2641, 2759, 2813
MfeI CAATTG 1 cut(s) 966
MhlI GDGCHC 4 cut(s) 538, 1551, 1928, 2088
MlsI TGGCCA 1 cut(s) 284
MluNI TGGCCA 1 cut(s) 284
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 2220
Mox20I TGGCCA 1 cut(s) 284
Mph1103I ATGCAT 4 cut(s) 1401, 1594, 1819, 2095
MroI TCCGGA 1 cut(s) 628
MroXI GAANNNNTTC 5 cut(s) 415, 550, 752, 772, 2398
MscI TGGCCA 1 cut(s) 284
MseI TTAA 7 cut(s) 503, 522, 579, 714, 2166, 2258, 2736
MslI CAYNNNNRTG 3 cut(s) 1268, 1331, 1827
Msp20I TGGCCA 1 cut(s) 284
MspA1I CMGCKG 2 cut(s) 327, 896
MspCI CTTAAG 1 cut(s) 2735
MspI CCGG 5 cut(s) 233, 539, 629, 981, 2060
MspR9I CCNGG 6 cut(s) 216, 281, 556, 651, 2162, 2382
MunI CAATTG 1 cut(s) 966
Mva1269I GAATGC 3 cut(s) 1111, 1592, 1819
MvaI CCWGG 6 cut(s) 216, 281, 556, 651, 2162, 2382
MvnI CGCG 1 cut(s) 820
MwoI GCNNNNNNNGC 7 cut(s) 25, 220, 569, 1770, 1923, 2099, 2699
NlaIV GGNNCC 6 cut(s) 18, 512, 1020, 1659, 1925, 2541
NmuCI GTSAC 5 cut(s) 351, 1436, 1843, 2641, 2759
NsiI ATGCAT 4 cut(s) 1401, 1594, 1819, 2095
NspV TTCGAA 5 cut(s) 71, 367, 1596, 2421, 2697
OliI CACNNNNGTG 1 cut(s) 1331
PagI TCATGA 4 cut(s) 46, 681, 1729, 1822
PasI CCCWGGG 1 cut(s) 2381
PctI GAATGC 3 cut(s) 1111, 1592, 1819
PdmI GAANNNNTTC 5 cut(s) 415, 550, 752, 772, 2398
PflFI GACNNNGTC 1 cut(s) 723
PflMI CCANNNNNTGG 2 cut(s) 1682, 1970
PleI GAGTC 1 cut(s) 197
PmaCI CACGTG 1 cut(s) 1546
PmlI CACGTG 1 cut(s) 1546
PpsI GAGTC 1 cut(s) 197
Ppu21I YACGTR 2 cut(s) 702, 1546
PpuMI RGGWCCY 2 cut(s) 510, 1019
Psp124BI GAGCTC 1 cut(s) 538
Psp5II RGGWCCY 2 cut(s) 510, 1019
Psp6I CCWGG 6 cut(s) 214, 279, 554, 649, 2160, 2380
PspCI CACGTG 1 cut(s) 1546
PspEI GGTNACC 1 cut(s) 2186
PspGI CCWGG 6 cut(s) 214, 279, 554, 649, 2160, 2380
PspN4I GGNNCC 6 cut(s) 18, 512, 1020, 1659, 1925, 2541
PspPI GGNCC 4 cut(s) 4, 510, 1019, 1355
PspPPI RGGWCCY 2 cut(s) 510, 1019
PstI CTGCAG 3 cut(s) 1333, 1479, 1772
PstNI CAGNNNCTG 1 cut(s) 280
PsyI GACNNNGTC 1 cut(s) 723
PvuII CAGCTG 2 cut(s) 327, 896
RsaI GTAC 4 cut(s) 704, 1473, 1840, 2207
RsaNI GTAC 4 cut(s) 703, 1472, 1839, 2206
RseI CAYNNNNRTG 3 cut(s) 1268, 1331, 1827
SacI GAGCTC 1 cut(s) 538
SaqAI TTAA 7 cut(s) 503, 522, 579, 714, 2166, 2258, 2736
Sau96I GGNCC 4 cut(s) 4, 510, 1019, 1355
ScaI AGTACT 1 cut(s) 1473
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 6 cut(s) 216, 281, 556, 651, 2162, 2382
SduI GDGCHC 4 cut(s) 538, 1551, 1928, 2088
SfcI CTRYAG 6 cut(s) 993, 1329, 1475, 1579, 1768, 1998
SfuI TTCGAA 5 cut(s) 71, 367, 1596, 2421, 2697
SinI GGWCC 4 cut(s) 4, 510, 1019, 1355
SmiMI CAYNNNNRTG 3 cut(s) 1268, 1331, 1827
SmlI CTYRAG 2 cut(s) 178, 2735
SmoI CTYRAG 2 cut(s) 178, 2735
SnaBI TACGTA 1 cut(s) 702
SsiI CCGC 8 cut(s) 64, 224, 257, 377, 740, 818, 1364, 2702
SspI AATATT 1 cut(s) 384
SspMI CTAG 6 cut(s) 147, 447, 480, 516, 1532, 1539
SstI GAGCTC 1 cut(s) 538
StyD4I CCNGG 6 cut(s) 214, 279, 554, 649, 2160, 2380
TaaI ACNGT 7 cut(s) 707, 1060, 1197, 1628, 1843, 2002, 2311
TaiI ACGT 3 cut(s) 704, 775, 1548
TaqI TCGA 9 cut(s) 57, 71, 367, 1162, 1596, 1737, 2421, 2697, 2804
TaqII GACCGA 1 cut(s) 1869
TatI WGTACW 3 cut(s) 1471, 1838, 2205
TauI GCSGC 3 cut(s) 226, 260, 742
Tru1I TTAA 7 cut(s) 503, 522, 579, 714, 2166, 2258, 2736
Tru9I TTAA 7 cut(s) 503, 522, 579, 714, 2166, 2258, 2736
TseFI GTSAC 5 cut(s) 351, 1436, 1843, 2641, 2759
TseI GCWGC 7 cut(s) 274, 1037, 1227, 1770, 2408, 2690, 2750
Tsp45I GTSAC 5 cut(s) 351, 1436, 1843, 2641, 2759
TspGWI ACGGA 1 cut(s) 1203
Tth111I GACNNNGTC 1 cut(s) 723
Van91I CCANNNNNTGG 2 cut(s) 1682, 1970
Vha464I CTTAAG 1 cut(s) 2735
VneI GTGCAC 2 cut(s) 1547, 2084
VpaK11BI GGWCC 4 cut(s) 4, 510, 1019, 1355
XagI CCTNNNNNAGG 1 cut(s) 1932
XapI RAATTY 9 cut(s) 53, 415, 1148, 1213, 1519, 1857, 1939, 2350, 2396
XbaI TCTAGA 3 cut(s) 146, 1531, 1538
XmiI GTMKAC 1 cut(s) 698
XmnI GAANNNNTTC 5 cut(s) 415, 550, 752, 772, 2398
XspI CTAG 6 cut(s) 147, 447, 480, 516, 1532, 1539
ZrmI AGTACT 1 cut(s) 1473
Zsp2I ATGCAT 4 cut(s) 1401, 1594, 1819, 2095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.