Rw7G027250

Plant calmodulin-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003606
Physical Location & Seq
Reverse (-)
35089542 .. 35091435
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G027250.1

Sequence Viewer

Length: 1680 bp
ATGGTGAAATTGCAAGCTCTAAGTCCTCGTAAAGCAAAGCCATCTAATAAGGAAATGAAGGAAAATGATTTCAAGCAAATGTTGTGTGATGACAATGAAACGCCTGCTCAACAAGAAGTGGGGATCAATTTCTTTGTGGAAATATTTGCTAAAAGGAAGGAAGATGATGCTCAGACATTTGTTGAGGATATACCTGAAGCAGAAATCATAGATTGCTTTACTGAAGAGGAGCAAAAAGAAGATGTGGCGGATAAAGAATACCAACCTCTTATGGATCAAGAAGAACCTGCCATGAGAAGCTGTTCGAGTGAGAGCGACTCTGATGGACTTTCGAGCATCGAAGTAGATTATGCCAATTCTGAAGCAACTGATATGGAGTGGGAGGAAGGGCAATTTTCCCTTGCAGTGCTGTATGATGATGATGATGATGAATCTAGTTCAAATGCTGGGTTTCATCAATTCAAGATGGTGATATGCATGAAGAACCTAAAACTCCATCTTTTGAGACCCAACTCAAAAATGATGGCACAAAACAGAATTTCTGGCATTGAAGAATCTGAAAGATTGAGCTATGACCAATTTTCTTACACTCAGGATTCATTTGAAGAAGACAGTGAACTATCAGAGACAGAAATAGAGATATCTTCATCTTCAATACAAGAGCCAACTGAGCGCCTAACAGCAACTAGTGAGGAAGTTCAAGAGAAAACCAGAGTTTTTGTAGCAGAGGATCATGAAATGGAGTCTCATCTTGGAGATGTTGAAAGACACTACACTGCTGCTTGTGAGACGAATGAAGCTTCAGATAGCCAACCAAGGAATGAATTTCAAGATAATGGAACAACTATGTCGACAAGTAACCACATTTCCAATGCTGCTCAGGATATCAGCACAACATATGAAGCTGAAACAAACGCAGAGAAAGAGGATTCAGAAACAGATAAAGTTGTTGTCACTGTTGTTTCTGGAAATCAAATCACAGAAAGTATTGTTGATGAAAACACTTTTGTTGACAGTAAAGATAACCTGGCCAATGGGGAGTACACAGATAATGCTAAAGCTGAATATGAATATCATATCTTCACAGAGAAGGATCAAAGTGAATTTAAGAAGGAAAAGATCTCAAGTTCCAGTGAAGGTGAGGATCAGAGTGATCTAAAGCTGAAGCAAATAGATGTAGCAGAAAACAGTGGCGGAGACATTGATAGAATGGAAGTGGACAACACTTCAGAGCCAGAAGCAGCAGAAACTTTCCTCATGGCCAATAGTAGCATCAGTCCAGGGATGAAGAGAAAGTTTTCCCATCAAGAAAGCAATTCGGATGAAGAACTGCAATACACAAACAGGAAATGGAAAATCAATTGCACGAGAAGTATCAAGGATGAGGAAGAACAATGGAAATATAATCAAAGAAAACCCAATTACCTGCCGTTGGTTCCTGGCCCTGAAGCAGAGAAGGTTGACTTGAGGCATCAGATAATAGATGAAAAGAAAAATGCAGAGGAATGGATGCTCGACTTTGCAATCCAACAAGCTGTAACCAAACTTGCTCCAGCTAGGAAGAAGAAGGTGGCATTGCTTGTTGAAGCTTTTGAGAAAGTCATGCCAGTGCCGCAATATGAACCCTGTTTGAAGCATTCTTCAGCAGCCTTCAGTCATGATAAGTCAAGTGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaM_binding

Protein Analysis

559

Amino Acids

63.64

Weight (kDa)

4.42

Isoelectric Point (pI)

56.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaM_binding PF07839 435 - 536 1.8e-28 Plant calmodulin-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 295, 1434
AccI GTMKAC 1 cut(s) 851
AciI CCGC 3 cut(s) 248, 1194, 1613
AclWI GGATC 5 cut(s) 131, 282, 738, 1101, 1152
AcoI YGGCCR 2 cut(s) 1029, 1260
AcsI RAATTY 3 cut(s) 537, 824, 1103
AcuI CTGAAG 9 cut(s) 216, 243, 381, 786, 1184, 1212, 1467, 1626, 1636
AfaI GTAC 1 cut(s) 1043
AfiI CCNNNNNNNGG 1 cut(s) 1432
AhlI ACTAGT 1 cut(s) 686
AjnI CCWGG 3 cut(s) 1026, 1279, 1438
Alw26I GTCTC 5 cut(s) 499, 620, 750, 782, 1191
AlwI GGATC 5 cut(s) 131, 282, 738, 1101, 1152
AoxI GGCC 3 cut(s) 1029, 1260, 1441
ApeKI GCWGC 4 cut(s) 779, 875, 1241, 1646
ApoI RAATTY 3 cut(s) 537, 824, 1103
Asp700I GAANNNNTTC 2 cut(s) 301, 1297
AspLEI GCGC 1 cut(s) 675
AspS9I GGNCC 1 cut(s) 1442
AsuHPI GGTGA 3 cut(s) 16, 481, 1151
BalI TGGCCA 2 cut(s) 1031, 1262
BauI CACGAG 1 cut(s) 1366
BbsI GAAGAC 1 cut(s) 615
BbvI GCAGC 4 cut(s) 766, 862, 1253, 1658
BccI CCATC 6 cut(s) 49, 317, 460, 504, 517, 1311
BceAI ACGGC 1 cut(s) 1414
BciT130I CCWGG 3 cut(s) 1028, 1281, 1440
BcoDI GTCTC 5 cut(s) 499, 620, 750, 782, 1191
BcuI ACTAGT 1 cut(s) 686
BfaI CTAG 3 cut(s) 435, 687, 1557
BfoI RGCGCY 1 cut(s) 676
BfuAI ACCTGC 2 cut(s) 295, 1434
BglII AGATCT 1 cut(s) 1119
BisI GCNGC 5 cut(s) 780, 876, 1242, 1613, 1647
BlsI GCNGC 5 cut(s) 781, 877, 1243, 1614, 1648
Bme1390I CCNGG 3 cut(s) 1028, 1281, 1440
BmgT120I GGNCC 1 cut(s) 1442
BmiI GGNNCC 1 cut(s) 1437
BmrFI CCNGG 3 cut(s) 1028, 1281, 1440
BmsI GCATC 5 cut(s) 157, 345, 1281, 1480, 1500
BpiI GAAGAC 1 cut(s) 615
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 1 cut(s) 1536
Bpu10I CCTNAGC 1 cut(s) 879
BpuEI CTTGAG 2 cut(s) 1108, 1486
BsaI GGTCTC 1 cut(s) 499
BsaJI CCNNGG 2 cut(s) 815, 1280
Bsc4I CCNNNNNNNGG 1 cut(s) 1432
Bse1I ACTGG 2 cut(s) 1131, 1607
Bse3DI GCAATG 1 cut(s) 1574
BseBI CCWGG 3 cut(s) 1028, 1281, 1440
BseDI CCNNGG 2 cut(s) 815, 1280
BseGI GGATG 4 cut(s) 1290, 1327, 1387, 1515
BseLI CCNNNNNNNGG 1 cut(s) 1432
BseMI GCAATG 1 cut(s) 1574
BseMII CTCAG 4 cut(s) 185, 605, 660, 893
BseNI ACTGG 2 cut(s) 1131, 1607
BseRI GAGGAG 1 cut(s) 242
BseXI GCAGC 4 cut(s) 766, 862, 1253, 1658
BseYI CCCAGC 1 cut(s) 446
BshFI GGCC 3 cut(s) 1031, 1262, 1443
BslI CCNNNNNNNGG 1 cut(s) 1432
BsmAI GTCTC 5 cut(s) 499, 620, 750, 782, 1191
BsmBI CGTCTC 1 cut(s) 782
BsmI GAATGC 1 cut(s) 1636
BsnI GGCC 3 cut(s) 1031, 1262, 1443
Bso31I GGTCTC 1 cut(s) 499
Bsp143I GATC 7 cut(s) 123, 274, 730, 1093, 1119, 1144, 1153
BspACI CCGC 3 cut(s) 248, 1194, 1613
BspANI GGCC 3 cut(s) 1031, 1262, 1443
BspCNI CTCAG 4 cut(s) 184, 604, 661, 892
BspHI TCATGA 2 cut(s) 733, 1657
BspLI GGNNCC 1 cut(s) 1437
BspMI ACCTGC 2 cut(s) 295, 1434
BspPI GGATC 5 cut(s) 131, 282, 738, 1101, 1152
BspTNI GGTCTC 1 cut(s) 499
BsrDI GCAATG 1 cut(s) 1574
BsrI ACTGG 2 cut(s) 1131, 1607
BssECI CCNNGG 2 cut(s) 815, 1280
BssMI GATC 7 cut(s) 123, 274, 730, 1093, 1119, 1144, 1153
BssSI CACGAG 1 cut(s) 1366
BssT1I CCWWGG 1 cut(s) 815
Bst2BI CACGAG 1 cut(s) 1366
Bst2UI CCWGG 3 cut(s) 1028, 1281, 1440
Bst4CI ACNGT 4 cut(s) 614, 958, 1016, 1190
Bst6I CTCTTC 2 cut(s) 219, 1283
BstC8I GCNNGC 2 cut(s) 15, 105
BstDEI CTNAG 5 cut(s) 20, 171, 591, 669, 879
BstF5I GGATG 4 cut(s) 1290, 1327, 1387, 1515
BstH2I RGCGCY 1 cut(s) 676
BstHHI GCGC 1 cut(s) 675
BstKTI GATC 7 cut(s) 126, 277, 733, 1096, 1122, 1147, 1156
BstMAI GTCTC 5 cut(s) 499, 620, 750, 782, 1191
BstMBI GATC 7 cut(s) 123, 274, 730, 1093, 1119, 1144, 1153
BstMWI GCNNNNNNNGC 2 cut(s) 670, 1612
BstNI CCWGG 3 cut(s) 1028, 1281, 1440
BstSCI CCNGG 3 cut(s) 1026, 1279, 1438
BstV1I GCAGC 4 cut(s) 766, 862, 1253, 1658
BstV2I GAAGAC 1 cut(s) 615
BstX2I RGATCY 1 cut(s) 1119
BstYI RGATCY 1 cut(s) 1119
BsuRI GGCC 3 cut(s) 1031, 1262, 1443
BtsCI GGATG 4 cut(s) 1290, 1327, 1387, 1515
BtsI GCAGTG 2 cut(s) 411, 774
BtsIMutI CAGTG 7 cut(s) 411, 619, 774, 954, 1138, 1195, 1614
BveI ACCTGC 2 cut(s) 295, 1434
Cac8I GCNNGC 2 cut(s) 15, 105
CciI TCATGA 2 cut(s) 733, 1657
CfoI GCGC 1 cut(s) 675
Cfr13I GGNCC 1 cut(s) 1442
Csp6I GTAC 1 cut(s) 1042
CviAII CATG 6 cut(s) 292, 478, 734, 1258, 1603, 1658
CviQI GTAC 1 cut(s) 1042
DdeI CTNAG 5 cut(s) 20, 171, 591, 669, 879
DpnI GATC 7 cut(s) 125, 276, 732, 1095, 1121, 1146, 1155
DpnII GATC 7 cut(s) 123, 274, 730, 1093, 1119, 1144, 1153
EaeI YGGCCR 2 cut(s) 1029, 1260
Eam1104I CTCTTC 2 cut(s) 219, 1283
EarI CTCTTC 2 cut(s) 219, 1283
EciI GGCGGA 2 cut(s) 263, 1209
Eco130I CCWWGG 1 cut(s) 815
Eco31I GGTCTC 1 cut(s) 499
Eco32I GATATC 2 cut(s) 642, 886
Eco57I CTGAAG 9 cut(s) 216, 243, 381, 786, 1184, 1212, 1467, 1626, 1636
EcoRII CCWGG 3 cut(s) 1026, 1279, 1438
EcoRV GATATC 2 cut(s) 642, 886
EcoT14I CCWWGG 1 cut(s) 815
EcoT22I ATGCAT 1 cut(s) 479
ErhI CCWWGG 1 cut(s) 815
Esp3I CGTCTC 1 cut(s) 782
FaeI CATG 6 cut(s) 295, 481, 737, 1261, 1606, 1661
FalI AAGNNNNNCTT 2 cut(s) 1448, 1480
FatI CATG 6 cut(s) 291, 477, 733, 1257, 1602, 1657
FauNDI CATATG 1 cut(s) 898
FblI GTMKAC 1 cut(s) 851
Fnu4HI GCNGC 5 cut(s) 780, 876, 1242, 1613, 1647
FokI GGATG 4 cut(s) 1297, 1334, 1394, 1522
Fsp4HI GCNGC 5 cut(s) 780, 876, 1242, 1613, 1647
FspBI CTAG 3 cut(s) 435, 687, 1557
GlaI GCGC 1 cut(s) 674
GluI GCNGC 5 cut(s) 780, 876, 1242, 1613, 1647
GsaI CCCAGC 1 cut(s) 450
GsuI CTGGAG 1 cut(s) 1536
HaeII RGCGCY 1 cut(s) 676
HaeIII GGCC 3 cut(s) 1031, 1262, 1443
HhaI GCGC 1 cut(s) 675
Hin1II CATG 6 cut(s) 295, 481, 737, 1261, 1606, 1661
Hin6I GCGC 1 cut(s) 673
HinP1I GCGC 1 cut(s) 673
HincII GTYRAC 3 cut(s) 852, 1012, 1462
HindII GTYRAC 3 cut(s) 852, 1012, 1462
HindIII AAGCTT 2 cut(s) 798, 1587
HinfI GANTC 6 cut(s) 317, 431, 554, 596, 743, 929
HphI GGTGA 3 cut(s) 16, 481, 1151
Hpy166II GTNNAC 6 cut(s) 617, 852, 1012, 1044, 1219, 1462
Hpy8I GTNNAC 6 cut(s) 617, 852, 1012, 1044, 1219, 1462
HpyAV CCTTC 9 cut(s) 52, 151, 380, 1084, 1105, 1130, 1450, 1561, 1660
HpyCH4III ACNGT 4 cut(s) 614, 958, 1016, 1190
HpyCH4V TGCA 8 cut(s) 13, 404, 477, 1333, 1365, 1499, 1523, 1673
HpyF10VI GCNNNNNNNGC 2 cut(s) 670, 1612
HpyF3I CTNAG 5 cut(s) 20, 171, 591, 669, 879
Hsp92II CATG 6 cut(s) 295, 481, 737, 1261, 1606, 1661
HspAI GCGC 1 cut(s) 673
Kzo9I GATC 7 cut(s) 123, 274, 730, 1093, 1119, 1144, 1153
LmnI GCTCC 2 cut(s) 229, 1555
Lsp1109I GCAGC 4 cut(s) 766, 862, 1253, 1658
LweI GCATC 5 cut(s) 157, 345, 1281, 1480, 1500
MaeI CTAG 3 cut(s) 435, 687, 1557
MaeIII GTNAC 3 cut(s) 857, 952, 1537
MalI GATC 7 cut(s) 125, 276, 732, 1095, 1121, 1146, 1155
MboI GATC 7 cut(s) 123, 274, 730, 1093, 1119, 1144, 1153
MfeI CAATTG 1 cut(s) 1360
MflI RGATCY 1 cut(s) 1119
MlsI TGGCCA 2 cut(s) 1031, 1262
MluNI TGGCCA 2 cut(s) 1031, 1262
MlyI GAGTC 2 cut(s) 311, 752
MmeI TCCRAC 1 cut(s) 1552
Mox20I TGGCCA 2 cut(s) 1031, 1262
Mph1103I ATGCAT 1 cut(s) 479
MroXI GAANNNNTTC 2 cut(s) 301, 1297
MscI TGGCCA 2 cut(s) 1031, 1262
MseI TTAA 1 cut(s) 1107
MslI CAYNNNNRTG 1 cut(s) 1607
Msp20I TGGCCA 2 cut(s) 1031, 1262
MspR9I CCNGG 3 cut(s) 1028, 1281, 1440
MunI CAATTG 1 cut(s) 1360
Mva1269I GAATGC 1 cut(s) 1636
MvaI CCWGG 3 cut(s) 1028, 1281, 1440
MwoI GCNNNNNNNGC 2 cut(s) 670, 1612
NdeI CATATG 1 cut(s) 898
NdeII GATC 7 cut(s) 123, 274, 730, 1093, 1119, 1144, 1153
NlaIII CATG 6 cut(s) 295, 481, 737, 1261, 1606, 1661
NlaIV GGNNCC 1 cut(s) 1437
NmuCI GTSAC 1 cut(s) 952
NsiI ATGCAT 1 cut(s) 479
PagI TCATGA 2 cut(s) 733, 1657
PctI GAATGC 1 cut(s) 1636
PdmI GAANNNNTTC 2 cut(s) 301, 1297
PfeI GAWTC 4 cut(s) 431, 554, 596, 929
PkrI GCNGC 5 cut(s) 781, 877, 1243, 1614, 1648
PleI GAGTC 2 cut(s) 311, 751
PpsI GAGTC 2 cut(s) 311, 751
Psp6I CCWGG 3 cut(s) 1026, 1279, 1438
PspFI CCCAGC 1 cut(s) 446
PspGI CCWGG 3 cut(s) 1026, 1279, 1438
PspN4I GGNNCC 1 cut(s) 1437
PspPI GGNCC 1 cut(s) 1442
PsuI RGATCY 1 cut(s) 1119
RsaI GTAC 1 cut(s) 1043
RsaNI GTAC 1 cut(s) 1042
RseI CAYNNNNRTG 1 cut(s) 1607
SalI GTCGAC 1 cut(s) 850
SaqAI TTAA 1 cut(s) 1107
SatI GCNGC 5 cut(s) 780, 876, 1242, 1613, 1647
Sau3AI GATC 7 cut(s) 123, 274, 730, 1093, 1119, 1144, 1153
Sau96I GGNCC 1 cut(s) 1442
SchI GAGTC 2 cut(s) 311, 752
ScrFI CCNGG 3 cut(s) 1028, 1281, 1440
SfaNI GCATC 5 cut(s) 157, 345, 1281, 1480, 1500
SmiMI CAYNNNNRTG 1 cut(s) 1607
SmlI CTYRAG 2 cut(s) 1123, 1465
SmoI CTYRAG 2 cut(s) 1123, 1465
SpeI ACTAGT 1 cut(s) 686
SsiI CCGC 3 cut(s) 248, 1194, 1613
SspI AATATT 1 cut(s) 144
SspMI CTAG 3 cut(s) 435, 687, 1557
StyD4I CCNGG 3 cut(s) 1026, 1279, 1438
StyI CCWWGG 1 cut(s) 815
TaaI ACNGT 4 cut(s) 614, 958, 1016, 1190
TaqI TCGA 5 cut(s) 305, 332, 339, 851, 1515
TatI WGTACW 1 cut(s) 1041
TauI GCSGC 1 cut(s) 1615
TfiI GAWTC 4 cut(s) 431, 554, 596, 929
Tru1I TTAA 1 cut(s) 1107
Tru9I TTAA 1 cut(s) 1107
TscAI CASTG 7 cut(s) 411, 619, 781, 961, 1138, 1195, 1614
TseFI GTSAC 1 cut(s) 952
TseI GCWGC 4 cut(s) 779, 875, 1241, 1646
Tsp45I GTSAC 1 cut(s) 952
TspRI CASTG 7 cut(s) 411, 619, 781, 961, 1138, 1195, 1614
XapI RAATTY 3 cut(s) 537, 824, 1103
XmiI GTMKAC 1 cut(s) 851
XmnI GAANNNNTTC 2 cut(s) 301, 1297
XspI CTAG 3 cut(s) 435, 687, 1557
Zsp2I ATGCAT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.