AT3G56250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004090
Physical Location & Seq
Forward (+)
20865913 .. 20868156
2244 bp
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UTR
Exon/CDS
Intron
AT3G56250.2

Sequence Viewer

Length: 669 bp
ATGGAAGCTCCTCGCTCCGTCGGAGGAAGAATACAGAGGAAACCGCTCGCGGATTGTACTAACACCGTCTCCAGATCTTCTCAACAGTCATCTTCTTCTGTAAAATTCGCGAATCCGAGTCTCACTTCTTCACTAAAGAGATTAGTAGAACAAACTACGCTCAAGGAGAAACCCAAAGATGTTAATACTTCGGCGACTGCTCCCGAAATCGCTTCTCGGCCTATTACCACGGACGTTAGACCCGTAACTCGCCGTATGTCTGCTGATCTGGCTTCTCCGGCCTCAGCTCCTTCTCGTCCCCAAACATCGCGATCTGATATGGGTGTTAGTGACAAGGATTTTACTGAGCCTTGGTCTGTTTACACGGTAAGAAGGAAGGCCTCTGGACAAAAAAGAAGTAAAGACGCATCTTCCTCTACTAGTGCAGCAGCTCGCATTCGTCTCGACTTGTCATCAAGCTCTGGGAAGAAAACACGTCAAGCTAGTGAAAACAAAAAGAAGACCTTGAAAGTGGCTCCAAAAAAGAGACAGCGGACGGTAAAGCAAGAGAAAGAGGATCCGGTTAGTGCAGCTTGTCAGGATTATATCGAGAAGCAGAAAGCTTACTTTGCAGAGATTGATGCATTTGAGCTGCCAGTAGAAGAGGTATCTAATAGCGACTCAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

24.3

Weight (kDa)

9.9

Isoelectric Point (pI)

73.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sororin_C PF25220 204 - 226 1.1e-11 Sororin C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 46
AccII CGCG 3 cut(s) 50, 110, 310
AciI CCGC 3 cut(s) 44, 50, 532
AclWI GGATC 2 cut(s) 551, 564
AcsI RAATTY 1 cut(s) 104
AfaI GTAC 1 cut(s) 58
AflIII ACRYGT 1 cut(s) 473
AgsI TTSAA 1 cut(s) 508
AhlI ACTAGT 1 cut(s) 419
AjiI CACGTC 1 cut(s) 476
AluBI AGCT 8 cut(s) 8, 287, 431, 459, 482, 572, 602, 631
AluI AGCT 8 cut(s) 8, 287, 431, 459, 482, 572, 602, 631
Alw26I GTCTC 4 cut(s) 73, 125, 446, 520
AlwI GGATC 2 cut(s) 551, 564
AoxI GGCC 3 cut(s) 218, 279, 378
ApeKI GCWGC 4 cut(s) 425, 428, 569, 631
ApoI RAATTY 1 cut(s) 104
BamHI GGATCC 1 cut(s) 556
BbsI GAAGAC 1 cut(s) 506
BbvCI CCTCAGC 1 cut(s) 283
BbvI GCAGC 4 cut(s) 437, 440, 581, 618
BceAI ACGGC 1 cut(s) 237
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BcoDI GTCTC 4 cut(s) 73, 125, 446, 520
BcuI ACTAGT 1 cut(s) 419
BfaI CTAG 2 cut(s) 420, 483
BglII AGATCT 1 cut(s) 74
BisI GCNGC 4 cut(s) 426, 429, 570, 632
BlsI GCNGC 4 cut(s) 427, 430, 571, 633
BmgBI CACGTC 1 cut(s) 476
BmiI GGNNCC 2 cut(s) 516, 558
BmsI GCATC 2 cut(s) 416, 610
BpiI GAAGAC 1 cut(s) 506
BpmI CTGGAG 1 cut(s) 55
Bpu10I CCTNAGC 1 cut(s) 283
BpuEI CTTGAG 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 228, 350
BsaWI WCCGGW 1 cut(s) 559
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bse1I ACTGG 1 cut(s) 635
BseDI CCNNGG 2 cut(s) 228, 350
BseMII CTCAG 2 cut(s) 297, 336
BseNI ACTGG 1 cut(s) 635
BseXI GCAGC 4 cut(s) 437, 440, 581, 618
BsgI GTGCAG 2 cut(s) 444, 588
Bsh1236I CGCG 3 cut(s) 50, 110, 310
BshFI GGCC 3 cut(s) 220, 281, 380
BsiSI CCGG 2 cut(s) 278, 560
BslFI GGGAC 1 cut(s) 282
BsmAI GTCTC 4 cut(s) 73, 125, 446, 520
BsmBI CGTCTC 2 cut(s) 73, 446
BsmFI GGGAC 1 cut(s) 282
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 3 cut(s) 220, 281, 380
Bsp143I GATC 4 cut(s) 74, 265, 311, 556
Bsp68I TCGCGA 2 cut(s) 110, 310
BspACI CCGC 3 cut(s) 44, 50, 532
BspANI GGCC 3 cut(s) 220, 281, 380
BspCNI CTCAG 2 cut(s) 296, 337
BspFNI CGCG 3 cut(s) 50, 110, 310
BspLI GGNNCC 2 cut(s) 516, 558
BspPI GGATC 2 cut(s) 551, 564
BsrBI CCGCTC 1 cut(s) 46
BsrI ACTGG 1 cut(s) 635
BssECI CCNNGG 2 cut(s) 228, 350
BssMI GATC 4 cut(s) 74, 265, 311, 556
BssT1I CCWWGG 1 cut(s) 350
Bst4CI ACNGT 4 cut(s) 67, 87, 367, 538
Bst6I CTCTTC 1 cut(s) 636
BstC8I GCNNGC 2 cut(s) 48, 433
BstDEI CTNAG 3 cut(s) 283, 345, 661
BstDSI CCRYGG 1 cut(s) 228
BstFNI CGCG 3 cut(s) 50, 110, 310
BstKTI GATC 4 cut(s) 77, 268, 314, 559
BstMAI GTCTC 4 cut(s) 73, 125, 446, 520
BstMBI GATC 4 cut(s) 74, 265, 311, 556
BstMWI GCNNNNNNNGC 3 cut(s) 269, 278, 608
BstUI CGCG 3 cut(s) 50, 110, 310
BstV1I GCAGC 4 cut(s) 437, 440, 581, 618
BstV2I GAAGAC 1 cut(s) 506
BstX2I RGATCY 2 cut(s) 74, 556
BstYI RGATCY 2 cut(s) 74, 556
BsuRI GGCC 3 cut(s) 220, 281, 380
BtgI CCRYGG 1 cut(s) 228
BtgZI GCGATG 1 cut(s) 291
BtrI CACGTC 1 cut(s) 476
BtuMI TCGCGA 2 cut(s) 110, 310
Cac8I GCNNGC 2 cut(s) 48, 433
CseI GACGC 1 cut(s) 413
Csp6I GTAC 1 cut(s) 57
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 3 cut(s) 283, 345, 661
DpnI GATC 4 cut(s) 76, 267, 313, 558
DpnII GATC 4 cut(s) 74, 265, 311, 556
Eam1104I CTCTTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 636
Eco130I CCWWGG 1 cut(s) 350
Eco147I AGGCCT 1 cut(s) 380
EcoT14I CCWWGG 1 cut(s) 350
EcoT22I ATGCAT 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 350
Esp3I CGTCTC 2 cut(s) 73, 446
FaiI YATR 3 cut(s) 257, 320, 585
FalI AAGNNNNNCTT 2 cut(s) 488, 520
FaqI GGGAC 1 cut(s) 282
Fnu4HI GCNGC 4 cut(s) 426, 429, 570, 632
Fsp4HI GCNGC 4 cut(s) 426, 429, 570, 632
FspBI CTAG 2 cut(s) 420, 483
GluI GCNGC 4 cut(s) 426, 429, 570, 632
GsuI CTGGAG 1 cut(s) 55
HaeIII GGCC 3 cut(s) 220, 281, 380
HapII CCGG 2 cut(s) 278, 560
HgaI GACGC 1 cut(s) 413
HindIII AAGCTT 1 cut(s) 600
HinfI GANTC 3 cut(s) 112, 118, 659
HpaII CCGG 2 cut(s) 278, 560
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 4 cut(s) 23, 117, 316, 664
Hpy188III TCNNGA 8 cut(s) 72, 109, 203, 309, 384, 443, 578, 589
Hpy8I GTNNAC 1 cut(s) 361
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 3 cut(s) 300, 366, 370
HpyCH4III ACNGT 4 cut(s) 67, 87, 367, 538
HpyCH4IV ACGT 2 cut(s) 234, 475
HpyCH4V TGCA 4 cut(s) 425, 569, 611, 623
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 278, 608
HpyF3I CTNAG 3 cut(s) 283, 345, 661
HpySE526I ACGT 2 cut(s) 234, 475
Kzo9I GATC 4 cut(s) 74, 265, 311, 556
LmnI GCTCC 5 cut(s) 13, 20, 205, 292, 520
LpnPI CCDG 8 cut(s) 85, 254, 291, 369, 447, 563, 573, 648
Lsp1109I GCAGC 4 cut(s) 437, 440, 581, 618
LweI GCATC 2 cut(s) 416, 610
MaeI CTAG 2 cut(s) 420, 483
MaeII ACGT 2 cut(s) 234, 475
MaeIII GTNAC 2 cut(s) 244, 329
MalI GATC 4 cut(s) 76, 267, 313, 558
MbiI CCGCTC 1 cut(s) 46
MboI GATC 4 cut(s) 74, 265, 311, 556
MboII GAAGA 9 cut(s) 39, 69, 84, 87, 120, 402, 478, 511, 653
MflI RGATCY 2 cut(s) 74, 556
MluCI AATT 1 cut(s) 104
MlyI GAGTC 2 cut(s) 127, 653
MnlI CCTC 8 cut(s) 17, 21, 30, 292, 391, 424, 547, 637
Mph1103I ATGCAT 1 cut(s) 625
MseI TTAA 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 532
MspI CCGG 2 cut(s) 278, 560
Mva1269I GAATGC 1 cut(s) 435
MvnI CGCG 3 cut(s) 50, 110, 310
MwoI GCNNNNNNNGC 3 cut(s) 269, 278, 608
NdeII GATC 4 cut(s) 74, 265, 311, 556
NlaIV GGNNCC 2 cut(s) 516, 558
NmeAIII GCCGAG 1 cut(s) 196
NmuCI GTSAC 1 cut(s) 329
NruI TCGCGA 2 cut(s) 110, 310
NsiI ATGCAT 1 cut(s) 625
PceI AGGCCT 1 cut(s) 380
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 1 cut(s) 435
PfeI GAWTC 1 cut(s) 112
PkrI GCNGC 4 cut(s) 427, 430, 571, 633
PleI GAGTC 2 cut(s) 126, 653
PpsI GAGTC 2 cut(s) 126, 653
PspN4I GGNNCC 2 cut(s) 516, 558
PsuI RGATCY 2 cut(s) 74, 556
RruI TCGCGA 2 cut(s) 110, 310
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 4 cut(s) 426, 429, 570, 632
Sau3AI GATC 4 cut(s) 74, 265, 311, 556
SchI GAGTC 2 cut(s) 127, 653
SfaNI GCATC 2 cut(s) 416, 610
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SpeI ACTAGT 1 cut(s) 419
Sse9I AATT 1 cut(s) 104
SseBI AGGCCT 1 cut(s) 380
SsiI CCGC 3 cut(s) 44, 50, 532
SspMI CTAG 2 cut(s) 420, 483
StuI AGGCCT 1 cut(s) 380
StyI CCWWGG 1 cut(s) 350
TaaI ACNGT 4 cut(s) 67, 87, 367, 538
TaiI ACGT 2 cut(s) 237, 478
TaqI TCGA 2 cut(s) 444, 588
TasI AATT 1 cut(s) 104
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 4 cut(s) 425, 428, 569, 631
Tsp45I GTSAC 1 cut(s) 329
TspGWI ACGGA 2 cut(s) 7, 245
XapI RAATTY 1 cut(s) 104
XspI CTAG 2 cut(s) 420, 483
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.