MD07G1230300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004090
Physical Location & Seq
Forward (+)
30481924 .. 30484692
2769 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1230300.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGAAGCTGAGCCATCCAGAAGAAGACTGAGAGCCCCAAACCCTCTCTCCGATTGCACCAACACCATTTCCTCCACCACCCTAGCCACTGCCACCTCTTCGCAGTCCTCGTCCGCCTCCACCGTCAAGCTCAAACGCCAGAATCCCAAGCTCGCCTCCGTCACCGGGAACCTCCTCTCCGCTTTAGACCCTAAGCCCTCCGTAGTTCCGCCTCCGATTCCCTCCACTCCTCCTCGCCCTCATCCCGTCTCGTCCTCCGCTTCTGGTACCAGCGATCGTGAGGCTCTTGAGCCTTGTTCAGTGTATACTCGAAGACATACTGCAGTAAAAGGGGAGGGTAAAGGGAAGGAAAACAACGGATCTTGGAGTTGTCCTACTGCACCAAAGACCCGGAATGCTGGGAAAAAAACAAAGGCAAGCGGATGCAACAGTGTGTCCAAGGAATCCATGGCTCCTCACCAAAAGAAGCAGCGTGCAGTACCAGCTGGGAAAGAGGATTGCGAGAAGCAGAGAGCTTTTTTTGATGAGATTGAACAAAAGAGAAAATATTTCGCAGAGATTGATGCATTTGAACTGTCAGAAGAGGAAGTCGATTCAGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

21.65

Weight (kDa)

9.22

Isoelectric Point (pI)

57.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 266
AccB1I GGYRCC 1 cut(s) 266
AccI GTMKAC 1 cut(s) 305
AciI CCGC 5 cut(s) 114, 180, 209, 258, 420
AclWI GGATC 1 cut(s) 367
AfaI GTAC 2 cut(s) 268, 480
AfiI CCNNNNNNNGG 1 cut(s) 165
AgsI TTSAA 2 cut(s) 533, 572
AloI GAACNNNNNNTCC 2 cut(s) 161, 193
AluBI AGCT 5 cut(s) 8, 130, 151, 485, 515
AluI AGCT 5 cut(s) 8, 130, 151, 485, 515
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 1 cut(s) 367
ApeKI GCWGC 1 cut(s) 469
Asp718I GGTACC 1 cut(s) 266
AsuC2I CCSGG 2 cut(s) 166, 391
AsuHPI GGTGA 2 cut(s) 154, 449
BanI GGYRCC 1 cut(s) 266
BanII GRGCYC 1 cut(s) 37
BbsI GAAGAC 2 cut(s) 31, 319
BbvI GCAGC 1 cut(s) 481
BccI CCATC 1 cut(s) 22
BcnI CCSGG 2 cut(s) 166, 391
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 1 cut(s) 83
BfmI CTRYAG 1 cut(s) 321
BisI GCNGC 1 cut(s) 470
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 1 cut(s) 471
Bme1390I CCNGG 2 cut(s) 166, 391
BmiI GGNNCC 3 cut(s) 170, 268, 453
BmrFI CCNGG 2 cut(s) 166, 391
BmsI GCATC 2 cut(s) 413, 553
BpiI GAAGAC 2 cut(s) 31, 319
Bpu10I CCTNAGC 1 cut(s) 192
Bpu1102I GCTNAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 2 cut(s) 166, 391
BsaJI CCNNGG 2 cut(s) 438, 447
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 165
BseDI CCNNGG 2 cut(s) 438, 447
BseGI GGATG 3 cut(s) 14, 241, 428
BseLI CCNNNNNNNGG 1 cut(s) 165
BseMII CTCAG 1 cut(s) 20
BseRI GAGGAG 4 cut(s) 164, 219, 222, 444
BseXI GCAGC 1 cut(s) 481
BseYI CCCAGC 2 cut(s) 398, 485
BsgI GTGCAG 2 cut(s) 363, 495
Bsh1285I CGRYCG 1 cut(s) 277
BshNI GGYRCC 1 cut(s) 266
BsiEI CGRYCG 1 cut(s) 277
BsiSI CCGG 2 cut(s) 165, 391
BslI CCNNNNNNNGG 1 cut(s) 165
BsmAI GTCTC 1 cut(s) 253
BsmBI CGTCTC 1 cut(s) 253
BsmI GAATGC 1 cut(s) 400
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 2 cut(s) 274, 359
Bsp1720I GCTNAGC 1 cut(s) 9
Bsp19I CCATGG 1 cut(s) 447
BspACI CCGC 5 cut(s) 114, 180, 209, 258, 420
BspCNI CTCAG 1 cut(s) 21
BspLI GGNNCC 3 cut(s) 170, 268, 453
BspMAI CTGCAG 1 cut(s) 325
BspPI GGATC 1 cut(s) 367
BspT107I GGYRCC 1 cut(s) 266
BssECI CCNNGG 2 cut(s) 438, 447
BssMI GATC 2 cut(s) 274, 359
BssNAI GTATAC 1 cut(s) 306
BssT1I CCWWGG 2 cut(s) 438, 447
Bst1107I GTATAC 1 cut(s) 306
Bst4CI ACNGT 3 cut(s) 124, 431, 576
Bst6I CTCTTC 2 cut(s) 103, 576
BstC8I GCNNGC 3 cut(s) 153, 418, 474
BstDEI CTNAG 3 cut(s) 9, 29, 192
BstDSI CCRYGG 1 cut(s) 447
BstF5I GGATG 3 cut(s) 14, 241, 428
BstKTI GATC 2 cut(s) 277, 362
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 2 cut(s) 274, 359
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstSCI CCNGG 2 cut(s) 164, 389
BstSFI CTRYAG 1 cut(s) 321
BstV1I GCAGC 1 cut(s) 481
BstV2I GAAGAC 2 cut(s) 31, 319
BstX2I RGATCY 1 cut(s) 359
BstYI RGATCY 1 cut(s) 359
BstZ17I GTATAC 1 cut(s) 306
BtgI CCRYGG 1 cut(s) 447
BtsCI GGATG 3 cut(s) 14, 241, 428
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 3 cut(s) 87, 306, 436
Cac8I GCNNGC 3 cut(s) 153, 418, 474
Csp6I GTAC 2 cut(s) 267, 479
CviAII CATG 1 cut(s) 448
CviQI GTAC 2 cut(s) 267, 479
DdeI CTNAG 3 cut(s) 9, 29, 192
DpnI GATC 2 cut(s) 276, 361
DpnII GATC 2 cut(s) 274, 359
Eam1104I CTCTTC 2 cut(s) 103, 576
EarI CTCTTC 2 cut(s) 103, 576
EciI GGCGGA 2 cut(s) 103, 198
Eco130I CCWWGG 2 cut(s) 438, 447
Eco24I GRGCYC 1 cut(s) 37
EcoT14I CCWWGG 2 cut(s) 438, 447
EcoT22I ATGCAT 1 cut(s) 568
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 2 cut(s) 438, 447
Esp3I CGTCTC 1 cut(s) 253
FaeI CATG 1 cut(s) 451
FaiI YATR 3 cut(s) 306, 318, 449
FatI CATG 1 cut(s) 447
FblI GTMKAC 1 cut(s) 305
Fnu4HI GCNGC 1 cut(s) 470
FokI GGATG 2 cut(s) 228, 435
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 1 cut(s) 470
FspBI CTAG 1 cut(s) 83
GluI GCNGC 1 cut(s) 470
GsaI CCCAGC 2 cut(s) 402, 489
HapII CCGG 2 cut(s) 165, 391
Hin1II CATG 1 cut(s) 451
HinfI GANTC 4 cut(s) 142, 217, 443, 593
HpaII CCGG 2 cut(s) 165, 391
HphI GGTGA 2 cut(s) 154, 449
Hpy166II GTNNAC 1 cut(s) 306
Hpy188I TCNGA 3 cut(s) 52, 216, 580
Hpy188III TCNNGA 3 cut(s) 18, 278, 287
Hpy8I GTNNAC 1 cut(s) 306
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 3 cut(s) 124, 431, 576
HpyCH4V TGCA 6 cut(s) 57, 323, 380, 426, 476, 566
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 3 cut(s) 9, 29, 192
Hsp92II CATG 1 cut(s) 451
KpnI GGTACC 1 cut(s) 270
Kzo9I GATC 2 cut(s) 274, 359
LmnI GCTCC 1 cut(s) 457
LpnPI CCDG 9 cut(s) 31, 152, 178, 249, 283, 384, 404, 471, 495
Lsp1109I GCAGC 1 cut(s) 481
LweI GCATC 2 cut(s) 413, 553
MaeI CTAG 1 cut(s) 83
MaeIII GTNAC 1 cut(s) 160
MalI GATC 2 cut(s) 276, 361
MboI GATC 2 cut(s) 274, 359
MboII GAAGA 5 cut(s) 33, 36, 90, 324, 593
MflI RGATCY 1 cut(s) 359
MhlI GDGCHC 1 cut(s) 37
Mph1103I ATGCAT 1 cut(s) 568
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 2 cut(s) 165, 391
MspR9I CCNGG 2 cut(s) 166, 391
Mva1269I GAATGC 1 cut(s) 400
MwoI GCNNNNNNNGC 1 cut(s) 482
NciI CCSGG 2 cut(s) 166, 391
NcoI CCATGG 1 cut(s) 447
NdeII GATC 2 cut(s) 274, 359
NlaIII CATG 1 cut(s) 451
NlaIV GGNNCC 3 cut(s) 170, 268, 453
NmuCI GTSAC 1 cut(s) 160
NsiI ATGCAT 1 cut(s) 568
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PctI GAATGC 1 cut(s) 400
PfeI GAWTC 4 cut(s) 142, 217, 443, 593
PkrI GCNGC 1 cut(s) 471
Ple19I CGATCG 1 cut(s) 277
PspFI CCCAGC 2 cut(s) 398, 485
PspN4I GGNNCC 3 cut(s) 170, 268, 453
PstI CTGCAG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 359
PvuI CGATCG 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 2 cut(s) 268, 480
RsaNI GTAC 2 cut(s) 267, 479
SatI GCNGC 1 cut(s) 470
Sau3AI GATC 2 cut(s) 274, 359
ScrFI CCNGG 2 cut(s) 166, 391
SduI GDGCHC 1 cut(s) 37
SetI ASST 7 cut(s) 10, 98, 132, 153, 174, 487, 517
SfaNI GCATC 2 cut(s) 413, 553
SfcI CTRYAG 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SsiI CCGC 5 cut(s) 114, 180, 209, 258, 420
SspI AATATT 1 cut(s) 548
SspMI CTAG 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 164, 389
StyI CCWWGG 2 cut(s) 438, 447
TaaI ACNGT 3 cut(s) 124, 431, 576
TaqI TCGA 2 cut(s) 310, 591
TfiI GAWTC 4 cut(s) 142, 217, 443, 593
TscAI CASTG 3 cut(s) 94, 306, 436
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 1 cut(s) 469
Tsp45I GTSAC 1 cut(s) 160
TspGWI ACGGA 3 cut(s) 148, 190, 372
TspRI CASTG 3 cut(s) 94, 306, 436
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XmiI GTMKAC 1 cut(s) 305
XspI CTAG 1 cut(s) 83
Zsp2I ATGCAT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.