MD01G1162300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004090
Physical Location & Seq
Forward (+)
26721916 .. 26724222
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1162300.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGAAGCTGAGCCATCGAGAAGAAGACTGAGGTCCCGAAACCCTCTCTCGGATTGCACCAACACCATTTCCTCCACCACCACCACCGCCGGCTCTTGTCAGTCCTCGTCCGCCTCCACCCCCGCGCTCAAACGCCAGAATCCCAAGCTCGCCTCCGTCACTAGGAACCTCTTCTCCGCTTTAACCCCTAAACCCTCTGCAGTCCCGCCGCCGATTCCGTCCACTCCTCCTCGTCCTCCTCCCGTCTCGTCCTCTGCTTCTGGTACCAGCGTTCGTGAGGCTCTTGAACCTTGTTCAGTGTATACTCGAAGACAGACTGCAGTAAAAAGGAAGGGTAAAGGGAAGGAAAATGCTGGATCCTGGAGTTGTCCTCCCGCACCAAAGACCCGGAATGCTAGGAAACAGACAAAGGAAAGCGGACACAACAGTGCATCCAAGGAATCAATGGCTCCTCACAAAAAGAAGCAGCGTGCAGTACCAGCTTCGGAGAAGCAGAGAGCTTACTTTGAAGAGATTGAGGAGAAGAGAAAATACTTCGCAGAGATCGATGCATTTGAACTGTCAGAAGAGGAAGTTGATTCAGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.38

Weight (kDa)

9.86

Isoelectric Point (pI)

78.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 263
AccI GTMKAC 1 cut(s) 302
AccII CGCG 1 cut(s) 125
AciI CCGC 8 cut(s) 87, 111, 123, 177, 206, 209, 375, 417
AclWI GGATC 2 cut(s) 351, 364
AfaI GTAC 2 cut(s) 265, 477
AfiI CCNNNNNNNGG 2 cut(s) 49, 162
AgsI TTSAA 3 cut(s) 287, 509, 557
AjnI CCWGG 1 cut(s) 359
AleI CACNNNNGTG 1 cut(s) 426
AloI GAACNNNNNNTCC 2 cut(s) 158, 190
AluBI AGCT 4 cut(s) 8, 148, 482, 500
AluI AGCT 4 cut(s) 8, 148, 482, 500
Alw26I GTCTC 1 cut(s) 250
AlwI GGATC 2 cut(s) 351, 364
ApeKI GCWGC 1 cut(s) 466
Asp700I GAANNNNTTC 1 cut(s) 170
Asp718I GGTACC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 1 cut(s) 33
AsuC2I CCSGG 1 cut(s) 388
AvaII GGWCC 1 cut(s) 33
BaeI ACNNNNGTAYC 2 cut(s) 255, 288
BamHI GGATCC 1 cut(s) 356
BanI GGYRCC 1 cut(s) 263
BarI GAAGNNNNNNTAC 2 cut(s) 515, 547
BbsI GAAGAC 2 cut(s) 31, 316
BbvI GCAGC 1 cut(s) 478
BccI CCATC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 361
BcnI CCSGG 1 cut(s) 388
BcoDI GTCTC 1 cut(s) 250
BfaI CTAG 2 cut(s) 162, 396
BfmI CTRYAG 2 cut(s) 198, 318
BisI GCNGC 2 cut(s) 209, 467
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 2 cut(s) 210, 468
Bme1390I CCNGG 2 cut(s) 361, 388
Bme18I GGWCC 1 cut(s) 33
BmgT120I GGNCC 1 cut(s) 33
BmiI GGNNCC 5 cut(s) 35, 167, 265, 358, 450
BmrFI CCNGG 2 cut(s) 361, 388
BmsI GCATC 2 cut(s) 440, 538
BoxI GACNNNNGTC 1 cut(s) 31
BpiI GAAGAC 2 cut(s) 31, 316
BplI GAGNNNNNCTC 2 cut(s) 355, 387
BpmI CTGGAG 1 cut(s) 382
Bpu1102I GCTNAGC 1 cut(s) 9
BpuMI CCSGG 1 cut(s) 388
Bsa29I ATCGAT 1 cut(s) 546
BsaJI CCNNGG 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 162
Bse118I RCCGGY 1 cut(s) 89
BseBI CCWGG 1 cut(s) 361
BseCI ATCGAT 1 cut(s) 546
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 1 cut(s) 431
BseLI CCNNNNNNNGG 2 cut(s) 49, 162
BseMII CTCAG 1 cut(s) 20
BseRI GAGGAG 5 cut(s) 216, 219, 228, 441, 533
BseXI GCAGC 1 cut(s) 478
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 125
BshNI GGYRCC 1 cut(s) 263
BshVI ATCGAT 1 cut(s) 546
BsiSI CCGG 2 cut(s) 90, 388
BslFI GGGAC 2 cut(s) 19, 188
BslI CCNNNNNNNGG 2 cut(s) 49, 162
BsmAI GTCTC 1 cut(s) 250
BsmBI CGTCTC 1 cut(s) 250
BsmFI GGGAC 2 cut(s) 19, 188
BsmI GAATGC 1 cut(s) 397
Bsp143I GATC 2 cut(s) 356, 543
Bsp1720I GCTNAGC 1 cut(s) 9
BspACI CCGC 8 cut(s) 87, 111, 123, 177, 206, 209, 375, 417
BspCNI CTCAG 1 cut(s) 21
BspDI ATCGAT 1 cut(s) 546
BspFNI CGCG 1 cut(s) 125
BspLI GGNNCC 5 cut(s) 35, 167, 265, 358, 450
BspMAI CTGCAG 2 cut(s) 202, 322
BspPI GGATC 2 cut(s) 351, 364
BspT107I GGYRCC 1 cut(s) 263
BsrFI RCCGGY 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 2 cut(s) 356, 543
BssNAI GTATAC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 435
Bst1107I GTATAC 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 361
Bst4CI ACNGT 2 cut(s) 428, 561
Bst6I CTCTTC 4 cut(s) 176, 504, 518, 561
BstC8I GCNNGC 3 cut(s) 91, 150, 471
BstDEI CTNAG 2 cut(s) 9, 29
BstF5I GGATG 1 cut(s) 431
BstFNI CGCG 1 cut(s) 125
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 2 cut(s) 359, 546
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 2 cut(s) 356, 543
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 1 cut(s) 361
BstPAI GACNNNNGTC 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 359, 386
BstSFI CTRYAG 2 cut(s) 198, 318
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 1 cut(s) 478
BstV2I GAAGAC 2 cut(s) 31, 316
BstX2I RGATCY 1 cut(s) 356
BstYI RGATCY 1 cut(s) 356
BstZ17I GTATAC 1 cut(s) 303
Bsu15I ATCGAT 1 cut(s) 546
BsuTUI ATCGAT 1 cut(s) 546
BtsCI GGATG 1 cut(s) 431
BtsIMutI CAGTG 2 cut(s) 303, 433
Cac8I GCNNGC 3 cut(s) 91, 150, 471
CfoI GCGC 1 cut(s) 127
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 33
ClaI ATCGAT 1 cut(s) 546
Csp6I GTAC 2 cut(s) 264, 476
CviJI RGCY 8 cut(s) 8, 13, 93, 148, 281, 449, 482, 500
CviKI_1 RGCY 8 cut(s) 8, 13, 93, 148, 281, 449, 482, 500
CviQI GTAC 2 cut(s) 264, 476
DdeI CTNAG 2 cut(s) 9, 29
DpnI GATC 2 cut(s) 358, 545
DpnII GATC 2 cut(s) 356, 543
Eam1104I CTCTTC 4 cut(s) 176, 504, 518, 561
EarI CTCTTC 4 cut(s) 176, 504, 518, 561
EciI GGCGGA 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 33
EcoO109I RGGNCCY 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 359
EcoT14I CCWWGG 1 cut(s) 435
EcoT22I ATGCAT 1 cut(s) 553
ErhI CCWWGG 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 250
FaiI YATR 1 cut(s) 303
FaqI GGGAC 2 cut(s) 19, 188
FauI CCCGC 3 cut(s) 130, 213, 382
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 2 cut(s) 209, 467
FokI GGATG 1 cut(s) 418
Fsp4HI GCNGC 2 cut(s) 209, 467
FspBI CTAG 2 cut(s) 162, 396
GlaI GCGC 1 cut(s) 126
GluI GCNGC 2 cut(s) 209, 467
GsuI CTGGAG 1 cut(s) 382
HapII CCGG 2 cut(s) 90, 388
HhaI GCGC 1 cut(s) 127
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HinfI GANTC 4 cut(s) 139, 214, 440, 578
HpaII CCGG 2 cut(s) 90, 388
Hpy166II GTNNAC 2 cut(s) 222, 303
Hpy188I TCNGA 3 cut(s) 52, 487, 565
Hpy188III TCNNGA 4 cut(s) 18, 36, 275, 284
Hpy8I GTNNAC 2 cut(s) 222, 303
HpyAV CCTTC 2 cut(s) 325, 337
HpyCH4III ACNGT 2 cut(s) 428, 561
HpyCH4V TGCA 6 cut(s) 57, 200, 320, 431, 473, 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 2 cut(s) 9, 29
HspAI GCGC 1 cut(s) 125
KpnI GGTACC 1 cut(s) 267
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
Kzo9I GATC 2 cut(s) 356, 543
LmnI GCTCC 1 cut(s) 454
LpnPI CCDG 9 cut(s) 103, 149, 246, 280, 339, 346, 373, 401, 492
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 2 cut(s) 440, 538
MaeI CTAG 2 cut(s) 162, 396
MaeIII GTNAC 1 cut(s) 157
MalI GATC 2 cut(s) 358, 545
MboI GATC 2 cut(s) 356, 543
MboII GAAGA 7 cut(s) 33, 36, 163, 321, 521, 535, 578
MflI RGATCY 1 cut(s) 356
Mph1103I ATGCAT 1 cut(s) 553
MroNI GCCGGC 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 1 cut(s) 182
MslI CAYNNNNRTG 1 cut(s) 426
MspI CCGG 2 cut(s) 90, 388
MspR9I CCNGG 2 cut(s) 361, 388
Mva1269I GAATGC 1 cut(s) 397
MvaI CCWGG 1 cut(s) 361
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 1 cut(s) 479
NaeI GCCGGC 1 cut(s) 91
NciI CCSGG 1 cut(s) 388
NdeII GATC 2 cut(s) 356, 543
NgoMIV GCCGGC 1 cut(s) 89
NlaIV GGNNCC 5 cut(s) 35, 167, 265, 358, 450
NmuCI GTSAC 1 cut(s) 157
NsiI ATGCAT 1 cut(s) 553
OliI CACNNNNGTG 1 cut(s) 426
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PctI GAATGC 1 cut(s) 397
PdiI GCCGGC 1 cut(s) 91
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 4 cut(s) 139, 214, 440, 578
PfoI TCCNGGA 1 cut(s) 359
PkrI GCNGC 2 cut(s) 210, 468
PpuMI RGGWCCY 1 cut(s) 33
PshAI GACNNNNGTC 1 cut(s) 31
Psp5II RGGWCCY 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 359
PspGI CCWGG 1 cut(s) 359
PspN4I GGNNCC 5 cut(s) 35, 167, 265, 358, 450
PspPI GGNCC 1 cut(s) 33
PspPPI RGGWCCY 1 cut(s) 33
PstI CTGCAG 2 cut(s) 202, 322
PsuI RGATCY 1 cut(s) 356
RsaI GTAC 2 cut(s) 265, 477
RsaNI GTAC 2 cut(s) 264, 476
RseI CAYNNNNRTG 1 cut(s) 426
SaqAI TTAA 1 cut(s) 182
SatI GCNGC 2 cut(s) 209, 467
Sau3AI GATC 2 cut(s) 356, 543
Sau96I GGNCC 1 cut(s) 33
ScrFI CCNGG 2 cut(s) 361, 388
SetI ASST 7 cut(s) 10, 35, 150, 171, 292, 484, 502
SfaNI GCATC 2 cut(s) 440, 538
SfcI CTRYAG 2 cut(s) 198, 318
SinI GGWCC 1 cut(s) 33
SmiMI CAYNNNNRTG 1 cut(s) 426
SsiI CCGC 8 cut(s) 87, 111, 123, 177, 206, 209, 375, 417
SspMI CTAG 2 cut(s) 162, 396
StyD4I CCNGG 2 cut(s) 359, 386
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 2 cut(s) 428, 561
TaqI TCGA 3 cut(s) 17, 307, 546
TauI GCSGC 1 cut(s) 211
TfiI GAWTC 4 cut(s) 139, 214, 440, 578
Tru1I TTAA 1 cut(s) 182
Tru9I TTAA 1 cut(s) 182
TscAI CASTG 2 cut(s) 303, 433
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 1 cut(s) 466
Tsp45I GTSAC 1 cut(s) 157
TspGWI ACGGA 2 cut(s) 145, 207
TspRI CASTG 2 cut(s) 303, 433
VpaK11BI GGWCC 1 cut(s) 33
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XmiI GTMKAC 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 2 cut(s) 162, 396
Zsp2I ATGCAT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.