AT4G00590

Asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004496
Physical Location & Seq
Forward (+)
252766 .. 255054
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00590.1

Sequence Viewer

Length: 1227 bp
ATGATGGCTGGCGACGGAGCAGATCAAAGTCGACGCTTTTTCGTGGCGGTTCACGTCGGGGCCGGATATCATGCGGCGGCTAACGAGAAAGCTCTTAGATCTGTCATGAGACGCGCTTGCCTAGCTGCGTCGACCATTCTTCGTCAGGATTCTGGTGAATGCATAGATGCTGTTTCAGCTGCCATTCAAGTCCTCGAGGATGACCCGAGCACAAATGCTGGTAGAGGCTCTAATCTGACAGAAGATGGTCATGTGGAATGTGATGCTAGTCTTATGGATGGAGATTCAGGGATGTTTGGTGGTGTTGGAGCAGTTCCAGGTGTCAGAAATGCTATCAAAATTGCTGCTTTGTTGGTGAAAGAGCAGATTACTGGCTCAACTCTCTTAGGCCGTATACCTCCTATGTTGTTGGTTGGTGAGGGAGCTCGACGTTGGGGTAAATCCAAAAGTGTTCTTATACCTGGAACTGTCACAGAAGCTGATCAGTGGCTGGTTACAGAGAGAGCAAGAAATCAGTGGAGAAGGTTCAAGGCCATGCTTTCTGAGGTAGGGGCAAAGAGTATTCTCTCTGCTGAAGAACATCCCAGAGGAACCGAGAATAATGAAACATGTGAGGAAAACGTATCATCCTGTGCAGCTGCAGATGAGGATAAGATCATGGACACTGTTGGCGTAATCTGTGTTGACAATGAAGGACACATTGCGTGTGGGTCCTCAAGTGGCGGTATTGCAATGAAGATTAGCGGCCGTGTAGGTTTGGCGGCAACATATGGTTCTGGTTGCTGGGCATCCTCTAAAGGACCATTTGGAGCTCCATTTCTAGTTGGATGTTGTGTGTCTGGAGCGGGAGAGTATCTTATGAGAGGTTTTGCAGCTAGAGAGTGTTGTACTTCATTGGCACTCTCTCAGGCTGGTCCAGCATCTGCTGCCATGAAGGTTTTACGTTCTGTAATGCATCAAGAGAGTTCAAAAATTGGAACAGCTGATAAAACTGCAGGAATTCTAGTAGTTCAGGCGGATGCTTCTGTGGTTGTCCCTGGAAGTAAGCCGGAGCTGAATGCTGTTGAGATAGCTGCTGCTTACTCGTCACTTTCTTTCGGCGTGGGATACTATGGGAACTCTATAGAGAAGCCAAAGATTTCGATTCTCAGGACGAGGAGACAGATGAGTGAGGCTGGGGTAGACCATTTTGAAGCCCGGATTGATCTCCGCCCAACCTGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

408

Amino Acids

42.63

Weight (kDa)

6.04

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 14 - 326 2.5e-45 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 194
AccBSI CCGCTC 1 cut(s) 845
AccI GTMKAC 4 cut(s) 31, 131, 394, 1182
AccII CGCG 1 cut(s) 114
AciI CCGC 9 cut(s) 47, 74, 77, 723, 744, 761, 845, 1016, 1210
AcoI YGGCCR 1 cut(s) 745
AcsI RAATTY 1 cut(s) 999
AcuI CTGAAG 1 cut(s) 594
AfaI GTAC 1 cut(s) 889
AflIII ACRYGT 1 cut(s) 608
AgsI TTSAA 4 cut(s) 188, 529, 969, 1193
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 3 cut(s) 316, 460, 1036
Alw21I GWGCWC 3 cut(s) 212, 427, 814
Alw26I GTCTC 2 cut(s) 103, 1153
AlwNI CAGNNNCTG 3 cut(s) 479, 490, 923
Ama87I CYCGRG 2 cut(s) 194, 205
AoxI GGCC 4 cut(s) 60, 388, 531, 745
ApeKI GCWGC 9 cut(s) 125, 179, 344, 635, 638, 872, 926, 1073, 1076
ApoI RAATTY 1 cut(s) 999
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 4 cut(s) 60, 711, 800, 914
AsuC2I CCSGG 1 cut(s) 1198
AsuHPI GGTGA 3 cut(s) 167, 367, 428
AvaI CYCGRG 2 cut(s) 194, 205
AvaII GGWCC 3 cut(s) 711, 800, 914
BanII GRGCYC 2 cut(s) 427, 814
Bbv12I GWGCWC 3 cut(s) 212, 427, 814
BbvI GCAGC 9 cut(s) 112, 166, 331, 625, 647, 884, 913, 1060, 1063
BccI CCATC 2 cut(s) 239, 272
BceAI ACGGC 2 cut(s) 375, 732
BciT130I CCWGG 3 cut(s) 318, 462, 1038
BciVI GTATCC 1 cut(s) 1100
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 1 cut(s) 1198
BcoDI GTCTC 2 cut(s) 103, 1153
BfaI CTAG 5 cut(s) 122, 267, 821, 876, 1004
BfmI CTRYAG 3 cut(s) 639, 993, 1122
BfuI GTATCC 1 cut(s) 1100
BglII AGATCT 1 cut(s) 98
Bme1390I CCNGG 4 cut(s) 318, 462, 1038, 1198
Bme18I GGWCC 3 cut(s) 711, 800, 914
BmeT110I CYCGRG 2 cut(s) 194, 205
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 4 cut(s) 60, 711, 800, 914
BmiI GGNNCC 3 cut(s) 61, 592, 712
BmrFI CCNGG 4 cut(s) 318, 462, 1038, 1198
BmsI GCATC 6 cut(s) 157, 253, 797, 929, 964, 1009
BpmI CTGGAG 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 700
BpuMI CCSGG 1 cut(s) 1198
BsaJI CCNNGG 1 cut(s) 1036
Bse1I ACTGG 1 cut(s) 376
Bse3DI GCAATG 2 cut(s) 699, 738
BseBI CCWGG 3 cut(s) 318, 462, 1038
BseDI CCNNGG 1 cut(s) 1036
BseGI GGATG 8 cut(s) 205, 283, 297, 580, 626, 788, 833, 1024
BseMI GCAATG 2 cut(s) 699, 738
BseMII CTCAG 3 cut(s) 534, 920, 1162
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 1 cut(s) 1171
BseX3I CGGCCG 1 cut(s) 745
BseXI GCAGC 9 cut(s) 112, 166, 331, 625, 647, 884, 913, 1060, 1063
BseYI CCCAGC 2 cut(s) 783, 1175
BsgI GTGCAG 1 cut(s) 654
Bsh1236I CGCG 1 cut(s) 114
Bsh1285I CGRYCG 1 cut(s) 748
BshFI GGCC 4 cut(s) 62, 390, 533, 747
BsiEI CGRYCG 1 cut(s) 748
BsiHKAI GWGCWC 3 cut(s) 212, 427, 814
BsiHKCI CYCGRG 2 cut(s) 194, 205
BsiSI CCGG 3 cut(s) 63, 1049, 1198
BslFI GGGAC 1 cut(s) 1019
BsmAI GTCTC 2 cut(s) 103, 1153
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 1019
BsmI GAATGC 2 cut(s) 164, 1063
BsnI GGCC 4 cut(s) 62, 390, 533, 747
BsoBI CYCGRG 2 cut(s) 194, 205
Bsp1286I GDGCHC 3 cut(s) 212, 427, 814
Bsp143I GATC 5 cut(s) 22, 98, 481, 654, 1204
BspACI CCGC 9 cut(s) 47, 74, 77, 723, 744, 761, 845, 1016, 1210
BspANI GGCC 4 cut(s) 62, 390, 533, 747
BspCNI CTCAG 3 cut(s) 535, 919, 1161
BspFNI CGCG 1 cut(s) 114
BspHI TCATGA 1 cut(s) 105
BspLI GGNNCC 3 cut(s) 61, 592, 712
BspMAI CTGCAG 2 cut(s) 643, 997
BsrBI CCGCTC 1 cut(s) 845
BsrDI GCAATG 2 cut(s) 699, 738
BsrI ACTGG 1 cut(s) 376
BssECI CCNNGG 1 cut(s) 1036
BssMI GATC 5 cut(s) 22, 98, 481, 654, 1204
BssNAI GTATAC 1 cut(s) 395
Bst1107I GTATAC 1 cut(s) 395
Bst2UI CCWGG 3 cut(s) 318, 462, 1038
Bst4CI ACNGT 2 cut(s) 469, 667
BstAPI GCANNNNNTGC 1 cut(s) 926
BstC8I GCNNGC 2 cut(s) 10, 118
BstDEI CTNAG 5 cut(s) 95, 385, 543, 906, 1148
BstF5I GGATG 8 cut(s) 205, 283, 297, 580, 626, 788, 833, 1024
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 5 cut(s) 25, 101, 484, 657, 1207
BstMAI GTCTC 2 cut(s) 103, 1153
BstMBI GATC 5 cut(s) 22, 98, 481, 654, 1204
BstMCI CGRYCG 1 cut(s) 748
BstMWI GCNNNNNNNGC 4 cut(s) 122, 176, 917, 926
BstNI CCWGG 3 cut(s) 318, 462, 1038
BstNSI RCATGY 1 cut(s) 612
BstSCI CCNGG 4 cut(s) 316, 460, 1036, 1196
BstSFI CTRYAG 3 cut(s) 639, 993, 1122
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 9 cut(s) 112, 166, 331, 625, 647, 884, 913, 1060, 1063
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
BstZ17I GTATAC 1 cut(s) 395
BstZI CGGCCG 1 cut(s) 745
BsuI GTATCC 1 cut(s) 1100
BsuRI GGCC 4 cut(s) 62, 390, 533, 747
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 8 cut(s) 205, 283, 297, 580, 626, 788, 833, 1024
BtsIMutI CAGTG 3 cut(s) 491, 521, 663
Cac8I GCNNGC 2 cut(s) 10, 118
CaiI CAGNNNCTG 3 cut(s) 479, 490, 923
CciI TCATGA 1 cut(s) 105
CfoI GCGC 1 cut(s) 116
Cfr13I GGNCC 4 cut(s) 60, 711, 800, 914
CseI GACGC 3 cut(s) 42, 117, 120
Csp6I GTAC 1 cut(s) 888
CviAII CATG 7 cut(s) 71, 106, 251, 535, 609, 658, 931
CviQI GTAC 1 cut(s) 888
DdeI CTNAG 5 cut(s) 95, 385, 543, 906, 1148
DpnI GATC 5 cut(s) 24, 100, 483, 656, 1206
DpnII GATC 5 cut(s) 22, 98, 481, 654, 1204
EaeI YGGCCR 1 cut(s) 745
EagI CGGCCG 1 cut(s) 745
EciI GGCGGA 2 cut(s) 1031, 1199
Ecl136II GAGCTC 2 cut(s) 425, 812
EclXI CGGCCG 1 cut(s) 745
Eco24I GRGCYC 2 cut(s) 427, 814
Eco32I GATATC 1 cut(s) 68
Eco47I GGWCC 3 cut(s) 711, 800, 914
Eco52I CGGCCG 1 cut(s) 745
Eco53kI GAGCTC 2 cut(s) 425, 812
Eco57I CTGAAG 1 cut(s) 594
Eco88I CYCGRG 2 cut(s) 194, 205
EcoICRI GAGCTC 2 cut(s) 425, 812
EcoO109I RGGNCCY 1 cut(s) 711
EcoRI GAATTC 1 cut(s) 999
EcoRII CCWGG 3 cut(s) 316, 460, 1036
EcoRV GATATC 1 cut(s) 68
EcoT22I ATGCAT 2 cut(s) 164, 957
EcoT38I GRGCYC 2 cut(s) 427, 814
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 7 cut(s) 74, 109, 254, 538, 612, 661, 934
FaqI GGGAC 1 cut(s) 1019
FatI CATG 7 cut(s) 70, 105, 250, 534, 608, 657, 930
FauI CCCGC 1 cut(s) 838
FauNDI CATATG 1 cut(s) 769
FbaI TGATCA 1 cut(s) 481
FblI GTMKAC 4 cut(s) 31, 131, 394, 1182
FokI GGATG 8 cut(s) 212, 290, 304, 567, 613, 775, 840, 1031
FriOI GRGCYC 2 cut(s) 427, 814
FspBI CTAG 5 cut(s) 122, 267, 821, 876, 1004
GlaI GCGC 1 cut(s) 115
GsaI CCCAGC 2 cut(s) 787, 1179
GsuI CTGGAG 1 cut(s) 861
HaeIII GGCC 4 cut(s) 62, 390, 533, 747
HapII CCGG 3 cut(s) 63, 1049, 1198
HgaI GACGC 3 cut(s) 42, 117, 120
HhaI GCGC 1 cut(s) 116
Hin1II CATG 7 cut(s) 74, 109, 254, 538, 612, 661, 934
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 3 cut(s) 32, 132, 685
HindII GTYRAC 3 cut(s) 32, 132, 685
HinfI GANTC 3 cut(s) 149, 284, 1144
HpaII CCGG 3 cut(s) 63, 1049, 1198
HphI GGTGA 3 cut(s) 167, 367, 428
Hpy166II GTNNAC 6 cut(s) 32, 52, 132, 395, 685, 1183
Hpy188I TCNGA 3 cut(s) 237, 326, 544
Hpy188III TCNNGA 5 cut(s) 106, 146, 840, 959, 1150
Hpy8I GTNNAC 6 cut(s) 32, 52, 132, 395, 685, 1183
Hpy99I CGWCG 5 cut(s) 17, 36, 59, 133, 432
HpyAV CCTTC 3 cut(s) 516, 686, 928
HpyCH4III ACNGT 2 cut(s) 469, 667
HpyCH4IV ACGT 4 cut(s) 54, 430, 621, 943
HpyCH4V TGCA 7 cut(s) 162, 635, 641, 731, 872, 955, 995
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 176, 917, 926
HpyF3I CTNAG 5 cut(s) 95, 385, 543, 906, 1148
HpySE526I ACGT 4 cut(s) 54, 430, 621, 943
Hsp92II CATG 7 cut(s) 74, 109, 254, 538, 612, 661, 934
HspAI GCGC 1 cut(s) 114
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 5 cut(s) 22, 98, 481, 654, 1204
LmnI GCTCC 7 cut(s) 17, 308, 422, 809, 817, 842, 1051
Lsp1109I GCAGC 9 cut(s) 112, 166, 331, 625, 647, 884, 913, 1060, 1063
LweI GCATC 6 cut(s) 157, 253, 797, 929, 964, 1009
MaeI CTAG 5 cut(s) 122, 267, 821, 876, 1004
MaeII ACGT 4 cut(s) 54, 430, 621, 943
MaeIII GTNAC 3 cut(s) 469, 493, 1086
MalI GATC 5 cut(s) 24, 100, 483, 656, 1206
MbiI CCGCTC 1 cut(s) 845
MboI GATC 5 cut(s) 22, 98, 481, 654, 1204
MboII GAAGA 4 cut(s) 131, 254, 587, 748
MflI RGATCY 1 cut(s) 98
MhlI GDGCHC 3 cut(s) 212, 427, 814
MluCI AATT 3 cut(s) 339, 972, 999
MmeI TCCRAC 2 cut(s) 286, 805
Mph1103I ATGCAT 2 cut(s) 164, 957
MspA1I CMGCKG 3 cut(s) 179, 638, 983
MspI CCGG 3 cut(s) 63, 1049, 1198
MspR9I CCNGG 4 cut(s) 318, 462, 1038, 1198
Mva1269I GAATGC 2 cut(s) 164, 1063
MvaI CCWGG 3 cut(s) 318, 462, 1038
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 4 cut(s) 122, 176, 917, 926
NciI CCSGG 1 cut(s) 1198
NdeI CATATG 1 cut(s) 769
NdeII GATC 5 cut(s) 22, 98, 481, 654, 1204
NlaIII CATG 7 cut(s) 74, 109, 254, 538, 612, 661, 934
NlaIV GGNNCC 3 cut(s) 61, 592, 712
NmuCI GTSAC 2 cut(s) 469, 1086
NsiI ATGCAT 2 cut(s) 164, 957
NspI RCATGY 1 cut(s) 612
PaeR7I CTCGAG 1 cut(s) 194
PagI TCATGA 1 cut(s) 105
PciI ACATGT 1 cut(s) 608
PctI GAATGC 2 cut(s) 164, 1063
PfeI GAWTC 3 cut(s) 149, 284, 1144
PpuMI RGGWCCY 1 cut(s) 711
PscI ACATGT 1 cut(s) 608
Psp124BI GAGCTC 2 cut(s) 427, 814
Psp5II RGGWCCY 1 cut(s) 711
Psp6I CCWGG 3 cut(s) 316, 460, 1036
PspFI CCCAGC 2 cut(s) 783, 1175
PspGI CCWGG 3 cut(s) 316, 460, 1036
PspN4I GGNNCC 3 cut(s) 61, 592, 712
PspPI GGNCC 4 cut(s) 60, 711, 800, 914
PspPPI RGGWCCY 1 cut(s) 711
PspXI VCTCGAGB 1 cut(s) 194
PstI CTGCAG 2 cut(s) 643, 997
PstNI CAGNNNCTG 3 cut(s) 479, 490, 923
PsuI RGATCY 1 cut(s) 98
PvuII CAGCTG 3 cut(s) 179, 638, 983
RsaI GTAC 1 cut(s) 889
RsaNI GTAC 1 cut(s) 888
SacI GAGCTC 2 cut(s) 427, 814
SalI GTCGAC 2 cut(s) 30, 130
Sau3AI GATC 5 cut(s) 22, 98, 481, 654, 1204
Sau96I GGNCC 4 cut(s) 60, 711, 800, 914
ScrFI CCNGG 4 cut(s) 318, 462, 1038, 1198
SduI GDGCHC 3 cut(s) 212, 427, 814
SfaNI GCATC 6 cut(s) 157, 253, 797, 929, 964, 1009
SfcI CTRYAG 3 cut(s) 639, 993, 1122
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 3 cut(s) 711, 800, 914
SlaI CTCGAG 1 cut(s) 194
SmlI CTYRAG 2 cut(s) 194, 715
SmoI CTYRAG 2 cut(s) 194, 715
Sse9I AATT 3 cut(s) 339, 972, 999
SsiI CCGC 9 cut(s) 47, 74, 77, 723, 744, 761, 845, 1016, 1210
SspMI CTAG 5 cut(s) 122, 267, 821, 876, 1004
SstI GAGCTC 2 cut(s) 427, 814
StyD4I CCNGG 4 cut(s) 316, 460, 1036, 1196
TaaI ACNGT 2 cut(s) 469, 667
TaiI ACGT 4 cut(s) 57, 433, 624, 946
TaqI TCGA 5 cut(s) 31, 131, 195, 427, 1142
TasI AATT 3 cut(s) 339, 972, 999
TatI WGTACW 1 cut(s) 887
TauI GCSGC 4 cut(s) 77, 80, 747, 764
TfiI GAWTC 3 cut(s) 149, 284, 1144
TscAI CASTG 3 cut(s) 491, 521, 670
TseFI GTSAC 2 cut(s) 469, 1086
TseI GCWGC 9 cut(s) 125, 179, 344, 635, 638, 872, 926, 1073, 1076
Tsp45I GTSAC 2 cut(s) 469, 1086
TspDTI ATGAA 5 cut(s) 618, 705, 749, 882, 947
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 3 cut(s) 491, 521, 670
VpaK11BI GGWCC 3 cut(s) 711, 800, 914
XapI RAATTY 1 cut(s) 999
XceI RCATGY 1 cut(s) 612
XhoI CTCGAG 1 cut(s) 194
XmiI GTMKAC 4 cut(s) 31, 131, 394, 1182
XspI CTAG 5 cut(s) 122, 267, 821, 876, 1004
Zsp2I ATGCAT 2 cut(s) 164, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.