pycom01g07920

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004496
Physical Location & Seq
Forward (+)
8903551 .. 8904889
1339 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g07920.1

Sequence Viewer

Length: 630 bp
ATGGTGGATGATGCAAAAGCAAATACAGAGGTTTCTGATTTTAGACTTTCTTGTATTCCTCAAGAGACCGCTGGAATATCAGAATTAGTAGCTCAACCATGCGATTCTTTGAAGCATAATGGAGCTGAAGAGGATTGCATCATGGATACTGTTGGAGTTATTTGTGTGGACGCTGAAGGACAAATAGCATCCGGCGCCTCAAGTGGGGGAATTGCACTGAAGGTTGGTGGACGTGTGGGATTAGCAGCAATGCATGGTGCAGGGTGTTGGGCGTCCTCCAAAGGTCTTTTTGGTGCTCCGTGTATAGTTGGCTGCTATACTAGTGGTGCTGGAGAATATGTAATGAAAGGATTTGCAGCTCACGAGTCCTGCGTCTCTTTGTCACTTTCACAGGCAGGTCCTGCCTCTGCTTGCACGAAAGTTTTATGCTCTGTTGTTCAGGAGAGTAATCATGATTGTACTGATAGAAGTGCCGGAATTCCACTTGTGCAAGCTGATGCTCCCATAACGGTTACAGGAAGGTCTCCAAAGTTGAAAGCTGTAGAGATTGGTGCTTGCTTACACGTCCTTATCCTTTGGAATAGGATATTTTGGGAGCTCTATGGAGCGGCCCAAGGTTTCAATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

210

Amino Acids

21.45

Weight (kDa)

5.0

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 36 - 150 6e-18 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 386
AccB1I GGYRCC 1 cut(s) 194
AccBSI CCGCTC 1 cut(s) 608
AciI CCGC 2 cut(s) 69, 608
AcsI RAATTY 1 cut(s) 477
AcuI CTGAAG 3 cut(s) 147, 195, 239
AcyI GRCGYC 2 cut(s) 195, 272
AfaI GTAC 1 cut(s) 460
AfiI CCNNNNNNNGG 1 cut(s) 204
AflIII ACRYGT 2 cut(s) 232, 562
AgsI TTSAA 3 cut(s) 112, 535, 622
AhlI ACTAGT 1 cut(s) 320
AjiI CACGTC 2 cut(s) 233, 565
AluBI AGCT 6 cut(s) 92, 125, 359, 494, 539, 598
AluI AGCT 6 cut(s) 92, 125, 359, 494, 539, 598
Alw21I GWGCWC 2 cut(s) 298, 600
Alw26I GTCTC 3 cut(s) 59, 379, 528
AlwNI CAGNNNCTG 1 cut(s) 401
AoxI GGCC 1 cut(s) 609
ApeKI GCWGC 3 cut(s) 245, 312, 356
ApoI RAATTY 1 cut(s) 477
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 2 cut(s) 398, 610
AvaII GGWCC 1 cut(s) 398
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 600
BauI CACGAG 1 cut(s) 362
Bbv12I GWGCWC 2 cut(s) 298, 600
BbvI GCAGC 3 cut(s) 257, 299, 368
BciVI GTATCC 1 cut(s) 139
BcoDI GTCTC 3 cut(s) 59, 379, 528
BcuI ACTAGT 1 cut(s) 320
BfaI CTAG 1 cut(s) 321
BfmI CTRYAG 1 cut(s) 540
BfoI RGCGCY 1 cut(s) 198
BfuAI ACCTGC 1 cut(s) 386
BfuI GTATCC 1 cut(s) 139
BisI GCNGC 4 cut(s) 246, 313, 357, 609
BlsI GCNGC 4 cut(s) 247, 314, 358, 610
Bme18I GGWCC 1 cut(s) 398
BmgBI CACGTC 2 cut(s) 233, 565
BmgT120I GGNCC 2 cut(s) 398, 610
BmiI GGNNCC 1 cut(s) 196
BmsI GCATC 3 cut(s) 147, 197, 487
BpmI CTGGAG 1 cut(s) 351
BpuEI CTTGAG 2 cut(s) 45, 184
BsaHI GRCGYC 2 cut(s) 195, 272
BsaI GGTCTC 2 cut(s) 59, 528
BsaJI CCNNGG 1 cut(s) 613
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 613
BseGI GGATG 2 cut(s) 13, 188
BseLI CCNNNNNNNGG 1 cut(s) 204
BseMI GCAATG 1 cut(s) 255
BseXI GCAGC 3 cut(s) 257, 299, 368
BsgI GTGCAG 1 cut(s) 279
BshFI GGCC 1 cut(s) 611
BshNI GGYRCC 1 cut(s) 194
BsiHKAI GWGCWC 2 cut(s) 298, 600
BsiSI CCGG 2 cut(s) 192, 474
BslI CCNNNNNNNGG 1 cut(s) 204
BsmAI GTCTC 3 cut(s) 59, 379, 528
BsmBI CGTCTC 1 cut(s) 379
BsnI GGCC 1 cut(s) 611
Bso31I GGTCTC 2 cut(s) 59, 528
Bsp1286I GDGCHC 2 cut(s) 298, 600
BspACI CCGC 2 cut(s) 69, 608
BspANI GGCC 1 cut(s) 611
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 1 cut(s) 196
BspMI ACCTGC 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 194
BspTNI GGTCTC 2 cut(s) 59, 528
BsrBI CCGCTC 1 cut(s) 608
BsrDI GCAATG 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 613
BssNI GRCGYC 2 cut(s) 195, 272
BssSI CACGAG 1 cut(s) 362
BssT1I CCWWGG 1 cut(s) 613
Bst2BI CACGAG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 151, 511
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 2 cut(s) 195, 272
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 3 cut(s) 412, 492, 556
BstDEI CTNAG 1 cut(s) 627
BstF5I GGATG 2 cut(s) 13, 188
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 1 cut(s) 197
BstMAI GTCTC 3 cut(s) 59, 379, 528
BstMWI GCNNNNNNNGC 2 cut(s) 194, 401
BstSFI CTRYAG 1 cut(s) 540
BstV1I GCAGC 3 cut(s) 257, 299, 368
BsuI GTATCC 1 cut(s) 139
BsuRI GGCC 1 cut(s) 611
BtrI CACGTC 2 cut(s) 233, 565
BtsCI GGATG 2 cut(s) 13, 188
BtsIMutI CAGTG 1 cut(s) 215
BveI ACCTGC 1 cut(s) 386
Cac8I GCNNGC 3 cut(s) 412, 492, 556
CaiI CAGNNNCTG 1 cut(s) 401
CciI TCATGA 1 cut(s) 451
CfoI GCGC 1 cut(s) 197
Cfr13I GGNCC 2 cut(s) 398, 610
CseI GACGC 3 cut(s) 179, 261, 361
Csp6I GTAC 1 cut(s) 459
CviAII CATG 4 cut(s) 99, 142, 254, 452
CviJI RGCY 8 cut(s) 92, 125, 312, 359, 494, 539, 598, 611
CviKI_1 RGCY 8 cut(s) 92, 125, 312, 359, 494, 539, 598, 611
CviQI GTAC 1 cut(s) 459
DdeI CTNAG 1 cut(s) 627
DinI GGCGCC 1 cut(s) 196
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 1 cut(s) 613
Eco24I GRGCYC 1 cut(s) 600
Eco31I GGTCTC 2 cut(s) 59, 528
Eco47I GGWCC 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 3 cut(s) 147, 195, 239
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 1 cut(s) 398
EcoRI GAATTC 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 613
EcoT22I ATGCAT 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 600
EgeI GGCGCC 1 cut(s) 196
EheI GGCGCC 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 613
Esp3I CGTCTC 1 cut(s) 379
FaeI CATG 4 cut(s) 102, 145, 257, 455
FatI CATG 4 cut(s) 98, 141, 253, 451
Fnu4HI GCNGC 4 cut(s) 246, 313, 357, 609
FokI GGATG 2 cut(s) 20, 175
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 4 cut(s) 246, 313, 357, 609
FspBI CTAG 1 cut(s) 321
GlaI GCGC 1 cut(s) 196
GluI GCNGC 4 cut(s) 246, 313, 357, 609
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 1 cut(s) 611
HapII CCGG 2 cut(s) 192, 474
HgaI GACGC 3 cut(s) 179, 261, 361
HhaI GCGC 1 cut(s) 197
Hin1I GRCGYC 2 cut(s) 195, 272
Hin1II CATG 4 cut(s) 102, 145, 257, 455
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HinfI GANTC 2 cut(s) 104, 365
HpaII CCGG 2 cut(s) 192, 474
Hpy166II GTNNAC 2 cut(s) 169, 230
Hpy188I TCNGA 2 cut(s) 37, 82
Hpy188III TCNNGA 4 cut(s) 62, 362, 440, 452
Hpy8I GTNNAC 2 cut(s) 169, 230
HpyAV CCTTC 3 cut(s) 170, 214, 513
HpyCH4III ACNGT 2 cut(s) 151, 511
HpyCH4IV ACGT 2 cut(s) 232, 564
HpyCH4V TGCA 8 cut(s) 14, 138, 215, 253, 260, 356, 414, 490
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 401
HpyF3I CTNAG 1 cut(s) 627
HpySE526I ACGT 2 cut(s) 232, 564
Hsp92I GRCGYC 2 cut(s) 195, 272
Hsp92II CATG 4 cut(s) 102, 145, 257, 455
HspAI GCGC 1 cut(s) 195
KasI GGCGCC 1 cut(s) 194
LmnI GCTCC 5 cut(s) 122, 301, 505, 595, 605
Lsp1109I GCAGC 3 cut(s) 257, 299, 368
LweI GCATC 3 cut(s) 147, 197, 487
MaeI CTAG 1 cut(s) 321
MaeII ACGT 2 cut(s) 232, 564
MaeIII GTNAC 2 cut(s) 381, 511
MbiI CCGCTC 1 cut(s) 608
MboII GAAGA 1 cut(s) 140
MhlI GDGCHC 2 cut(s) 298, 600
MluCI AATT 4 cut(s) 83, 210, 477, 622
Mly113I GGCGCC 1 cut(s) 195
MlyI GAGTC 1 cut(s) 374
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 6 cut(s) 22, 69, 124, 208, 286, 415
Mph1103I ATGCAT 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 2 cut(s) 192, 474
MwoI GCNNNNNNNGC 2 cut(s) 194, 401
NarI GGCGCC 1 cut(s) 195
NlaIII CATG 4 cut(s) 102, 145, 257, 455
NlaIV GGNNCC 1 cut(s) 196
NmuCI GTSAC 1 cut(s) 381
NsiI ATGCAT 1 cut(s) 255
PagI TCATGA 1 cut(s) 451
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 4 cut(s) 247, 314, 358, 610
PleI GAGTC 1 cut(s) 373
PluTI GGCGCC 1 cut(s) 198
PpsI GAGTC 1 cut(s) 373
PpuMI RGGWCCY 1 cut(s) 398
Psp124BI GAGCTC 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 398
PspN4I GGNNCC 1 cut(s) 196
PspPI GGNCC 2 cut(s) 398, 610
PspPPI RGGWCCY 1 cut(s) 398
PstNI CAGNNNCTG 1 cut(s) 401
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
SacI GAGCTC 1 cut(s) 600
SatI GCNGC 4 cut(s) 246, 313, 357, 609
Sau96I GGNCC 2 cut(s) 398, 610
SchI GAGTC 1 cut(s) 374
SduI GDGCHC 2 cut(s) 298, 600
SfaNI GCATC 3 cut(s) 147, 197, 487
SfcI CTRYAG 1 cut(s) 540
SfoI GGCGCC 1 cut(s) 196
SinI GGWCC 1 cut(s) 398
SmlI CTYRAG 2 cut(s) 60, 199
SmoI CTYRAG 2 cut(s) 60, 199
SpeI ACTAGT 1 cut(s) 320
Sse9I AATT 4 cut(s) 83, 210, 477, 622
SsiI CCGC 2 cut(s) 69, 608
SspDI GGCGCC 1 cut(s) 194
SspMI CTAG 1 cut(s) 321
SstI GAGCTC 1 cut(s) 600
StyI CCWWGG 1 cut(s) 613
TaaI ACNGT 2 cut(s) 151, 511
TaiI ACGT 2 cut(s) 235, 567
TasI AATT 4 cut(s) 83, 210, 477, 622
TatI WGTACW 1 cut(s) 458
TauI GCSGC 1 cut(s) 611
TfiI GAWTC 1 cut(s) 104
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 1 cut(s) 381
TseI GCWGC 3 cut(s) 245, 312, 356
Tsp45I GTSAC 1 cut(s) 381
TspDTI ATGAA 1 cut(s) 359
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 1 cut(s) 222
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 1 cut(s) 477
XspI CTAG 1 cut(s) 321
Zsp2I ATGCAT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.