Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0004496

Physical Location & Seq

Genomic Coordinates Forward (+)

20460867 .. 20464074

Gene Structure 3208 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000028242

Sequence Viewer

CDS Protein Promoter (2k)

Length: 993 bp

ATGGCTGCTGCCGCTAAGCAAGTACAAGAGCAACTAGAAGTTGAAAACGACAGATTCTTCGTCGCCGTTCATGTCGGCGCTGGATACCACGCGCCTTCCAAAGAGAAGGCCCTCAGGTCCGCCATGAAACGCACCTGCCTCGCTGCCGCTTCCATTCTCCGCCAGCTTTTGTCTATAACTGTTTGTTCTTGTAGATGCATAGATGCTGTCACTGCAGCTATTCAAGTGTTGGAGGGGTATGATCACGAACGAGGGCCGGCGCGGGGGTCTAATTTGACCGAAGATGGTTGTGTAGAATTTGATGCCAGTATTATGGACGGGAATTCTGGAGCATTTGGAGCTGTTGGAGCATTGTCAGGTGTCAAAAACCCCATCAAGGTTGCTGCTTTACTGGCCAAGGAGCAAATGATGGGATCCTCCCAGTTGGGCCGAATACCTCCCATAGTCCCATGCTCTTTGATTTGTAACTTCCTTGTTGGTGAAGGTGCCCGCATATGGGCGAAGTCAAAGGGCATTGCTTTGTCGGCAAGCACATCAGCTGCTGATGAGTGGCTGGTGACAGAAAGGGCAAAAGCACATTGGAAAAAGTACAAAGCAATGGTAGATGATGCAAAAGCAAAGACAGAAACTTCTGCTGTCGGACTTCCTTGTAGTCCTCAAGAGGCTGCTGGAATTCCAGAATTAGAAGCTCAACTATGTGATTCTTTGAAGAAAAATGAAGCAGAGGAGGATTGCATCATGGACACTGTTGGAGTTATTTGTGTGGATGCTGATGGACAAATTGCATCTGGTGCTTCAAGTGGGGACATTGCATTGAAAGTTGGTGGGCATGTAGGGTTAGTAGCAATGCATGGTTCAGGCTGTTGGGCATCCTCCAAAGGTCATTTTGGGGCTCCATGTATAGTTGGCTGTTGTGTAAGTGGTGCTGGAGAATATCTGATGAAAGGATTTGCAGCTCGAGTGTGCTGTGTCTCCTTATCACTGTATGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asparaginase_2

Protein Analysis

331

Amino Acids

34.27

Weight (kDa)

6.0

Isoelectric Point (pI)

21.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asparaginase_2	PF01112	20 - 133	2.2e-16	Asparaginase
Asparaginase_2	PF01112	157 - 324	1.4e-14	Asparaginase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004496)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00590 AT4G00590
fragaria_vesca	FvH4_7g13631 FvH4_7g13631
malus_domestica	MD01G1053500.v1.1 MD01G1053600.v1.1 MD07G1141600.v1.1
prunus_persica	Prupe.2G162500_v2.0.a1 Prupe.2G162500_v2.0.a1 Prupe.2G162500_v2.0.a1 Prupe.2G162500_v2.0.a1 Prupe.2G162500_v2.0.a1 Prupe.2G162500_v2.0.a1 Prupe.8G034000_v2.0.a1
pyrus_communis	pycom01g07920 pycom07g14050
rosa_chinensis	RchiOBHm_Chr1g0353681 RchiOBHm_Chr1g0353691
rosa_laevigata	RLG00000028242 RLG00000028265 RLG00000029492 RLG00000029775
rosa_multiflora	Rmu_sc0001849.1_g000001
rosa_roxburghii	Rroxscaffold_4G00301740
rosa_rugosa	Rorug01G0237100
rosa_samantha	Rh1AG249300 Rh1BG219400 Rh1CG099800 Rh1CG232000 Rh1DG107100 Rh1DG246400
rosa_wichuraiana	Rw1G021730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	143
Acc36I	ACCTGC	1 cut(s)	143
AccB1I	GGYRCC	1 cut(s)	485
AccII	CGCG	2 cut(s)	92, 262
AciI	CCGC	6 cut(s)	12, 120, 147, 160, 262, 490
AclWI	GGATC	2 cut(s)	408, 421
AcoI	YGGCCR	1 cut(s)	393
AcsI	RAATTY	3 cut(s)	296, 322, 672
AfaI	GTAC	2 cut(s)	24, 590
AfiI	CCNNNNNNNGG	4 cut(s)	263, 376, 495, 496
AgsI	TTSAA	5 cut(s)	44, 224, 709, 798, 817
AluBI	AGCT	6 cut(s)	166, 218, 341, 539, 689, 956
AluI	AGCT	6 cut(s)	166, 218, 341, 539, 689, 956
Alw26I	GTCTC	1 cut(s)	976
AlwI	GGATC	2 cut(s)	408, 421
AlwNI	CAGNNNCTG	1 cut(s)	542
Ama87I	CYCGRG	1 cut(s)	957
AoxI	GGCC	4 cut(s)	108, 254, 393, 427
ApeKI	GCWGC	8 cut(s)	5, 8, 143, 215, 383, 539, 665, 953
ApoI	RAATTY	3 cut(s)	296, 322, 672
ArsI	GACNNNNNNTTYG	2 cut(s)	41, 73
AspLEI	GCGC	3 cut(s)	80, 94, 262
AspS9I	GGNCC	4 cut(s)	109, 117, 254, 427
AsuHPI	GGTGA	2 cut(s)	491, 568
AvaI	CYCGRG	1 cut(s)	957
AvaII	GGWCC	1 cut(s)	117
AxyI	CCTNAGG	1 cut(s)	113
BaeGI	GKGCMC	1 cut(s)	490
BalI	TGGCCA	1 cut(s)	395
BamHI	GGATCC	1 cut(s)	413
BanI	GGYRCC	1 cut(s)	485
BanII	GRGCYC	1 cut(s)	895
BbvI	GCAGC	6 cut(s)	130, 227, 370, 526, 652, 965
BccI	CCATC	4 cut(s)	278, 380, 403, 767
BceAI	ACGGC	1 cut(s)	50
BcgI	CGANNNNNNTGC	2 cut(s)	121, 155
BciVI	GTATCC	1 cut(s)	77
BclI	TGATCA	1 cut(s)	241
BcoDI	GTCTC	1 cut(s)	976
BfaI	CTAG	1 cut(s)	35
BfmI	CTRYAG	1 cut(s)	213
BfoI	RGCGCY	1 cut(s)	81
BfuAI	ACCTGC	1 cut(s)	143
BfuI	GTATCC	1 cut(s)	77
BlpI	GCTNAGC	1 cut(s)	15
Bme18I	GGWCC	1 cut(s)	117
BmeT110I	CYCGRG	1 cut(s)	957
BmgT120I	GGNCC	4 cut(s)	109, 117, 254, 427
BmiI	GGNNCC	3 cut(s)	415, 487, 894
BmrI	ACTGGG	1 cut(s)	415
BmsI	GCATC	8 cut(s)	185, 193, 292, 598, 744, 757, 794, 878
BmuI	ACTGGG	1 cut(s)	415
BpmI	CTGGAG	2 cut(s)	348, 948
Bpu1102I	GCTNAGC	1 cut(s)	15
BpuEI	CTTGAG	1 cut(s)	642
BsaJI	CCNNGG	1 cut(s)	396
Bsc4I	CCNNNNNNNGG	4 cut(s)	263, 376, 495, 496
Bse118I	RCCGGY	1 cut(s)	256
Bse1I	ACTGG	3 cut(s)	306, 396, 421
Bse21I	CCTNAGG	1 cut(s)	113
Bse3DI	GCAATG	4 cut(s)	513, 603, 807, 852
BseDI	CCNNGG	1 cut(s)	396
BseGI	GGATG	2 cut(s)	772, 869
BseLI	CCNNNNNNNGG	4 cut(s)	263, 376, 495, 496
BseMI	GCAATG	4 cut(s)	513, 603, 807, 852
BseMII	CTCAG	1 cut(s)	127
BseNI	ACTGG	3 cut(s)	306, 396, 421
BseRI	GAGGAG	1 cut(s)	740
BseSI	GKGCMC	1 cut(s)	490
BseXI	GCAGC	6 cut(s)	130, 227, 370, 526, 652, 965
Bsh1236I	CGCG	2 cut(s)	92, 262
BshFI	GGCC	4 cut(s)	110, 256, 395, 429
BshNI	GGYRCC	1 cut(s)	485
BsiHKCI	CYCGRG	1 cut(s)	957
BsiSI	CCGG	1 cut(s)	257
BslFI	GGGAC	2 cut(s)	431, 818
BslI	CCNNNNNNNGG	4 cut(s)	263, 376, 495, 496
BsmAI	GTCTC	1 cut(s)	976
BsmFI	GGGAC	2 cut(s)	431, 818
BsnI	GGCC	4 cut(s)	110, 256, 395, 429
BsoBI	CYCGRG	1 cut(s)	957
Bsp1286I	GDGCHC	2 cut(s)	490, 895
Bsp143I	GATC	2 cut(s)	241, 413
Bsp1720I	GCTNAGC	1 cut(s)	15
BspACI	CCGC	6 cut(s)	12, 120, 147, 160, 262, 490
BspANI	GGCC	4 cut(s)	110, 256, 395, 429
BspCNI	CTCAG	1 cut(s)	126
BspFNI	CGCG	2 cut(s)	92, 262
BspLI	GGNNCC	3 cut(s)	415, 487, 894
BspMAI	CTGCAG	1 cut(s)	217
BspMI	ACCTGC	1 cut(s)	143
BspPI	GGATC	2 cut(s)	408, 421
BspT107I	GGYRCC	1 cut(s)	485
BsrDI	GCAATG	4 cut(s)	513, 603, 807, 852
BsrFI	RCCGGY	1 cut(s)	256
BsrI	ACTGG	3 cut(s)	306, 396, 421
BssAI	RCCGGY	1 cut(s)	256
BssECI	CCNNGG	1 cut(s)	396
BssMI	GATC	2 cut(s)	241, 413
BssT1I	CCWWGG	1 cut(s)	396
Bst4CI	ACNGT	3 cut(s)	181, 748, 984
BstAPI	GCANNNNNTGC	1 cut(s)	791
BstC8I	GCNNGC	4 cut(s)	164, 258, 490, 529
BstDEI	CTNAG	2 cut(s)	15, 113
BstF5I	GGATG	2 cut(s)	772, 869
BstFNI	CGCG	2 cut(s)	92, 262
BstH2I	RGCGCY	1 cut(s)	81
BstHHI	GCGC	3 cut(s)	80, 94, 262
BstKTI	GATC	2 cut(s)	244, 416
BstMAI	GTCTC	1 cut(s)	976
BstMBI	GATC	2 cut(s)	241, 413
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 212, 338, 347, 392, 524, 791
BstNSI	RCATGY	1 cut(s)	833
BstSFI	CTRYAG	1 cut(s)	213
BstSLI	GKGCMC	1 cut(s)	490
BstUI	CGCG	2 cut(s)	92, 262
BstV1I	GCAGC	6 cut(s)	130, 227, 370, 526, 652, 965
BstX2I	RGATCY	1 cut(s)	413
BstXI	CCANNNNNNTGG	1 cut(s)	313
BstYI	RGATCY	1 cut(s)	413
Bsu36I	CCTNAGG	1 cut(s)	113
BsuI	GTATCC	1 cut(s)	77
BsuRI	GGCC	4 cut(s)	110, 256, 395, 429
BtsCI	GGATG	2 cut(s)	772, 869
BtsI	GCAGTG	1 cut(s)	210
BtsIMutI	CAGTG	3 cut(s)	210, 744, 980
BveI	ACCTGC	1 cut(s)	143
Cac8I	GCNNGC	4 cut(s)	164, 258, 490, 529
CaiI	CAGNNNCTG	1 cut(s)	542
CfoI	GCGC	3 cut(s)	80, 94, 262
Cfr10I	RCCGGY	1 cut(s)	256
Cfr13I	GGNCC	4 cut(s)	109, 117, 254, 427
Csp6I	GTAC	2 cut(s)	23, 589
CviAII	CATG	7 cut(s)	71, 124, 450, 739, 830, 851, 897
CviQI	GTAC	2 cut(s)	23, 589
DdeI	CTNAG	2 cut(s)	15, 113
DpnI	GATC	2 cut(s)	243, 415
DpnII	GATC	2 cut(s)	241, 413
EaeI	YGGCCR	1 cut(s)	393
EciI	GGCGGA	2 cut(s)	109, 149
Eco130I	CCWWGG	1 cut(s)	396
Eco24I	GRGCYC	1 cut(s)	895
Eco47I	GGWCC	1 cut(s)	117
Eco81I	CCTNAGG	1 cut(s)	113
Eco88I	CYCGRG	1 cut(s)	957
EcoO109I	RGGNCCY	1 cut(s)	109
EcoRI	GAATTC	2 cut(s)	322, 672
EcoT14I	CCWWGG	1 cut(s)	396
EcoT22I	ATGCAT	2 cut(s)	200, 852
EcoT38I	GRGCYC	1 cut(s)	895
ErhI	CCWWGG	1 cut(s)	396
FaeI	CATG	7 cut(s)	74, 127, 453, 742, 833, 854, 900
FaqI	GGGAC	2 cut(s)	431, 818
FatI	CATG	7 cut(s)	70, 123, 449, 738, 829, 850, 896
FauI	CCCGC	2 cut(s)	255, 497
FauNDI	CATATG	1 cut(s)	494
FbaI	TGATCA	1 cut(s)	241
FokI	GGATG	2 cut(s)	779, 856
FriOI	GRGCYC	1 cut(s)	895
FspBI	CTAG	1 cut(s)	35
GlaI	GCGC	3 cut(s)	79, 93, 261
GsuI	CTGGAG	2 cut(s)	348, 948
HaeII	RGCGCY	1 cut(s)	81
HaeIII	GGCC	4 cut(s)	110, 256, 395, 429
HapII	CCGG	1 cut(s)	257
HhaI	GCGC	3 cut(s)	80, 94, 262
Hin1II	CATG	7 cut(s)	74, 127, 453, 742, 833, 854, 900
Hin6I	GCGC	3 cut(s)	78, 92, 260
HinP1I	GCGC	3 cut(s)	78, 92, 260
HinfI	GANTC	2 cut(s)	54, 701
HpaII	CCGG	1 cut(s)	257
HphI	GGTGA	2 cut(s)	491, 568
Hpy188I	TCNGA	2 cut(s)	641, 939
Hpy188III	TCNNGA	4 cut(s)	245, 327, 659, 677
Hpy99I	CGWCG	1 cut(s)	65
HpyAV	CCTTC	3 cut(s)	100, 105, 476
HpyCH4III	ACNGT	3 cut(s)	181, 748, 984
HpyCH4V	TGCA	8 cut(s)	198, 215, 611, 735, 785, 812, 850, 953
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 212, 338, 347, 392, 524, 791
HpyF3I	CTNAG	2 cut(s)	15, 113
Hsp92II	CATG	7 cut(s)	74, 127, 453, 742, 833, 854, 900
HspAI	GCGC	3 cut(s)	78, 92, 260
KroI	GCCGGC	1 cut(s)	256
KroNI	GCCGGC	1 cut(s)	258
Ksp22I	TGATCA	1 cut(s)	241
Kzo9I	GATC	2 cut(s)	241, 413
LmnI	GCTCC	5 cut(s)	329, 338, 347, 400, 898
Lsp1109I	GCAGC	6 cut(s)	130, 227, 370, 526, 652, 965
LweI	GCATC	8 cut(s)	185, 193, 292, 598, 744, 757, 794, 878
MaeI	CTAG	1 cut(s)	35
MaeIII	GTNAC	3 cut(s)	208, 464, 556
MalI	GATC	2 cut(s)	243, 415
MboI	GATC	2 cut(s)	241, 413
MboII	GAAGA	3 cut(s)	49, 293, 721
MflI	RGATCY	1 cut(s)	413
MhlI	GDGCHC	2 cut(s)	490, 895
MlsI	TGGCCA	1 cut(s)	395
MluCI	AATT	6 cut(s)	271, 296, 322, 672, 680, 780
MluNI	TGGCCA	1 cut(s)	395
MmeI	TCCRAC	4 cut(s)	210, 325, 619, 730
Mox20I	TGGCCA	1 cut(s)	395
Mph1103I	ATGCAT	2 cut(s)	200, 852
MroNI	GCCGGC	1 cut(s)	256
MscI	TGGCCA	1 cut(s)	395
Msp20I	TGGCCA	1 cut(s)	395
MspA1I	CMGCKG	1 cut(s)	539
MspI	CCGG	1 cut(s)	257
MvnI	CGCG	2 cut(s)	92, 262
MwoI	GCNNNNNNNGC	7 cut(s)	11, 212, 338, 347, 392, 524, 791
NaeI	GCCGGC	1 cut(s)	258
NdeI	CATATG	1 cut(s)	494
NdeII	GATC	2 cut(s)	241, 413
NgoMIV	GCCGGC	1 cut(s)	256
NlaIII	CATG	7 cut(s)	74, 127, 453, 742, 833, 854, 900
NlaIV	GGNNCC	3 cut(s)	415, 487, 894
NmuCI	GTSAC	2 cut(s)	208, 556
NsiI	ATGCAT	2 cut(s)	200, 852
NspI	RCATGY	1 cut(s)	833
PaeR7I	CTCGAG	1 cut(s)	957
PaqCI	CACCTGC	1 cut(s)	143
PdiI	GCCGGC	1 cut(s)	258
PfeI	GAWTC	2 cut(s)	54, 701
PspN4I	GGNNCC	3 cut(s)	415, 487, 894
PspPI	GGNCC	4 cut(s)	109, 117, 254, 427
PspXI	VCTCGAGB	1 cut(s)	957
PstI	CTGCAG	1 cut(s)	217
PstNI	CAGNNNCTG	1 cut(s)	542
PsuI	RGATCY	1 cut(s)	413
PvuII	CAGCTG	1 cut(s)	539
RsaI	GTAC	2 cut(s)	24, 590
RsaNI	GTAC	2 cut(s)	23, 589
Sau3AI	GATC	2 cut(s)	241, 413
Sau96I	GGNCC	4 cut(s)	109, 117, 254, 427
SduI	GDGCHC	2 cut(s)	490, 895
SfaNI	GCATC	8 cut(s)	185, 193, 292, 598, 744, 757, 794, 878
SfcI	CTRYAG	1 cut(s)	213
Sfr274I	CTCGAG	1 cut(s)	957
SinI	GGWCC	1 cut(s)	117
SlaI	CTCGAG	1 cut(s)	957
SmlI	CTYRAG	2 cut(s)	657, 957
SmoI	CTYRAG	2 cut(s)	657, 957
Sse9I	AATT	6 cut(s)	271, 296, 322, 672, 680, 780
SsiI	CCGC	6 cut(s)	12, 120, 147, 160, 262, 490
SspMI	CTAG	1 cut(s)	35
StyI	CCWWGG	1 cut(s)	396
TaaI	ACNGT	3 cut(s)	181, 748, 984
TaqI	TCGA	1 cut(s)	958
TaqII	GACCGA	1 cut(s)	293
TasI	AATT	6 cut(s)	271, 296, 322, 672, 680, 780
TatI	WGTACW	2 cut(s)	22, 588
TauI	GCSGC	2 cut(s)	14, 149
TfiI	GAWTC	2 cut(s)	54, 701
TscAI	CASTG	3 cut(s)	217, 751, 987
TseFI	GTSAC	2 cut(s)	208, 556
TseI	GCWGC	8 cut(s)	5, 8, 143, 215, 383, 539, 665, 953
Tsp45I	GTSAC	2 cut(s)	208, 556
TspDTI	ATGAA	4 cut(s)	59, 140, 732, 956
TspRI	CASTG	3 cut(s)	217, 751, 987
VpaK11BI	GGWCC	1 cut(s)	117
XapI	RAATTY	3 cut(s)	296, 322, 672
XceI	RCATGY	1 cut(s)	833
XhoI	CTCGAG	1 cut(s)	957
XspI	CTAG	1 cut(s)	35
Zsp2I	ATGCAT	2 cut(s)	200, 852

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.