Rh1AG249300

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004496
Physical Location & Seq
Forward (+)
46357820 .. 46361726
3907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG249300.1

Sequence Viewer

Length: 1254 bp
ATGGCTGCTGCCGCTAAACAAGTACAACAGCAACTGCTAGAAGATGAAAACGACAGATTCTTCGTCGCCGTTCATGTCGGCGCTGGATACCACGCGCCTTCCAACGAGAAGGCCCTCAGGTCCGCCATGAAACGCGCCTGCCTCGCCGCCGCTTCCATTCTCCGCCAGGGCTCTGGTAGGTGCATAGATGCTGTCACTGCAGCTATTCAAGTGTTGGAGGATGATCCAATCACGAACGCAGGCCGGGGGTCTAATTTGACTGAAGATGGTCGTGTAGAATGTGATGCCAGTATTATGGATGGGAATTCTGGACTATTTGGAGCTGTTGGAGCATTGCCAGGTGTCAAAAACCCCATCAAGGTTGCTGCTTTACTGGCCAAGGAGCAAATGATGGGATCCTCCCAGTTGGGCCGAATACCTCCCATCTTCCTCGTTGGTGAAGGTGCCCGCATATGGGCGAAGTCCAAGGGCATTGCTTTGTCGGCAAGCACATCAGCTGCTGATGAGTGGCTGGTAACAGCAAGGGCAAAAGCACATTGGAAAAAGTACAAAGCAATGGTAGATGATGCAAAAGCAAAGACAGAAACTTCTGCTGGTGGACTTCCTTGTAGTCCTCAAGAGGCTGCTGGAATTCCAGAAGATCAACCATGTGATTTTTTGAATAAAAATGGGGCAGAGGAGGACATCATGGACACTGTAGGAGTTATTTGTGTAGATGCTGACGGACAAATTGCATCTGGTGCCTCAAGTGGAGGCATTGCATTAAAGGTTGGTGGTCGTGTAGGGTTAGCAGCAATGCATGGTTCAGGCTGTTGGGCATCCTCCAAAGGTTTTTTTGGGGCTCCATGTATAGTCGGTTGTTGTGTAAGTGGTGCTGGAGAATATCTGATGAAAGGATTTGCAGCTCGAGAGTGCTGTGTCTCTTTATCACTTTCACAGGCAGGTCCTGCCTCTGCTTGCATGAAAGTTTTACGCTCCTTTGTTCAAGAGAGTAATCAAAATTGTACTGATAGAAGTGCCGGATTTCTAGCTGTGCAAGCTGATGCTCCTGTGATGGTTTCAGGAAGTTCTCCAAAGTTAAAAGCTATTGAGATTGCAGCTGCTTACTCTGCCTTATCCTTTGGGATAGGATTTTTTGGAAGCTCTATGGAGCGACCCAAGGTTTCAATTCTCAGGAGTACAAAATTGCAAACCAAAACTGGAGTAGACCATTTTGAAGCACGAATTGATCTTTCTCCTAAAAGGAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

417

Amino Acids

43.21

Weight (kDa)

8.14

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 21 - 348 1.1e-47 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 932
AccB1I GGYRCC 2 cut(s) 443, 740
AccI GTMKAC 1 cut(s) 1206
AccII CGCG 2 cut(s) 95, 135
AciI CCGC 6 cut(s) 12, 123, 147, 150, 163, 448
AclWI GGATC 3 cut(s) 218, 390, 403
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 2 cut(s) 304, 630
AcuI CTGAAG 1 cut(s) 282
AfaI GTAC 4 cut(s) 24, 548, 1006, 1180
AfiI CCNNNNNNNGG 3 cut(s) 358, 453, 454
AgsI TTSAA 5 cut(s) 209, 661, 986, 1167, 1217
AjnI CCWGG 2 cut(s) 165, 337
Alw26I GTCTC 1 cut(s) 925
AlwI GGATC 3 cut(s) 218, 390, 403
AlwNI CAGNNNCTG 3 cut(s) 34, 500, 947
Ama87I CYCGRG 1 cut(s) 906
AoxI GGCC 4 cut(s) 111, 241, 375, 409
ApoI RAATTY 2 cut(s) 304, 630
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspLEI GCGC 3 cut(s) 83, 97, 137
AspS9I GGNCC 4 cut(s) 112, 120, 409, 944
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 449
AvaI CYCGRG 1 cut(s) 906
AvaII GGWCC 2 cut(s) 120, 944
AxyI CCTNAGG 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 448
BalI TGGCCA 1 cut(s) 377
BamHI GGATCC 1 cut(s) 395
BanI GGYRCC 2 cut(s) 443, 740
BanII GRGCYC 2 cut(s) 173, 844
BbvI GCAGC 8 cut(s) 212, 352, 484, 610, 803, 914, 1087, 1109
BccI CCATC 6 cut(s) 260, 293, 362, 385, 431, 1048
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 2 cut(s) 167, 339
BciVI GTATCC 1 cut(s) 80
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 1 cut(s) 925
BfaI CTAG 2 cut(s) 38, 1028
BfmI CTRYAG 2 cut(s) 198, 696
BfoI RGCGCY 1 cut(s) 84
BfuAI ACCTGC 1 cut(s) 932
BfuI GTATCC 1 cut(s) 80
Bme1390I CCNGG 3 cut(s) 167, 245, 339
Bme18I GGWCC 2 cut(s) 120, 944
BmeT110I CYCGRG 1 cut(s) 906
BmgT120I GGNCC 4 cut(s) 112, 120, 409, 944
BmiI GGNNCC 4 cut(s) 397, 445, 742, 843
BmrFI CCNGG 3 cut(s) 167, 245, 339
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 7 cut(s) 178, 274, 556, 706, 743, 827, 1033
BmuI ACTGGG 1 cut(s) 397
BpmI CTGGAG 2 cut(s) 897, 1221
BpuEI CTTGAG 2 cut(s) 600, 730
BpuMI CCSGG 1 cut(s) 245
BsaJI CCNNGG 5 cut(s) 166, 244, 378, 465, 1158
Bsc4I CCNNNNNNNGG 3 cut(s) 358, 453, 454
Bse1I ACTGG 4 cut(s) 288, 378, 403, 1204
Bse21I CCTNAGG 1 cut(s) 116
Bse3DI GCAATG 5 cut(s) 332, 471, 561, 756, 801
BseBI CCWGG 2 cut(s) 167, 339
BseDI CCNNGG 5 cut(s) 166, 244, 378, 465, 1158
BseGI GGATG 3 cut(s) 226, 304, 818
BseLI CCNNNNNNNGG 3 cut(s) 358, 453, 454
BseMI GCAATG 5 cut(s) 332, 471, 561, 756, 801
BseMII CTCAG 2 cut(s) 130, 1186
BseNI ACTGG 4 cut(s) 288, 378, 403, 1204
BseRI GAGGAG 1 cut(s) 692
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 8 cut(s) 212, 352, 484, 610, 803, 914, 1087, 1109
Bsh1236I CGCG 2 cut(s) 95, 135
BshFI GGCC 4 cut(s) 113, 243, 377, 411
BshNI GGYRCC 2 cut(s) 443, 740
BsiHKCI CYCGRG 1 cut(s) 906
BsiSI CCGG 2 cut(s) 244, 1020
BslI CCNNNNNNNGG 3 cut(s) 358, 453, 454
BsmAI GTCTC 1 cut(s) 925
BsnI GGCC 4 cut(s) 113, 243, 377, 411
BsoBI CYCGRG 1 cut(s) 906
Bsp1286I GDGCHC 3 cut(s) 173, 448, 844
Bsp143I GATC 4 cut(s) 223, 395, 640, 1228
BspACI CCGC 6 cut(s) 12, 123, 147, 150, 163, 448
BspANI GGCC 4 cut(s) 113, 243, 377, 411
BspCNI CTCAG 2 cut(s) 129, 1185
BspFNI CGCG 2 cut(s) 95, 135
BspLI GGNNCC 4 cut(s) 397, 445, 742, 843
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 932
BspPI GGATC 3 cut(s) 218, 390, 403
BspT107I GGYRCC 2 cut(s) 443, 740
BsrDI GCAATG 5 cut(s) 332, 471, 561, 756, 801
BsrI ACTGG 4 cut(s) 288, 378, 403, 1204
BssECI CCNNGG 5 cut(s) 166, 244, 378, 465, 1158
BssMI GATC 4 cut(s) 223, 395, 640, 1228
BssT1I CCWWGG 3 cut(s) 378, 465, 1158
Bst2UI CCWGG 2 cut(s) 167, 339
Bst4CI ACNGT 1 cut(s) 697
BstAPI GCANNNNNTGC 2 cut(s) 740, 947
BstC8I GCNNGC 6 cut(s) 139, 241, 448, 487, 958, 1038
BstDEI CTNAG 2 cut(s) 116, 1172
BstF5I GGATG 3 cut(s) 226, 304, 818
BstFNI CGCG 2 cut(s) 95, 135
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 3 cut(s) 83, 97, 137
BstKTI GATC 4 cut(s) 226, 398, 643, 1231
BstMAI GTCTC 1 cut(s) 925
BstMBI GATC 4 cut(s) 223, 395, 640, 1228
BstNI CCWGG 2 cut(s) 167, 339
BstSCI CCNGG 3 cut(s) 165, 243, 337
BstSFI CTRYAG 2 cut(s) 198, 696
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 2 cut(s) 95, 135
BstV1I GCAGC 8 cut(s) 212, 352, 484, 610, 803, 914, 1087, 1109
BstX2I RGATCY 1 cut(s) 395
BstXI CCANNNNNNTGG 2 cut(s) 173, 295
BstYI RGATCY 1 cut(s) 395
Bsu36I CCTNAGG 1 cut(s) 116
BsuI GTATCC 1 cut(s) 80
BsuRI GGCC 4 cut(s) 113, 243, 377, 411
BtsCI GGATG 3 cut(s) 226, 304, 818
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 195, 693
BveI ACCTGC 1 cut(s) 932
Cac8I GCNNGC 6 cut(s) 139, 241, 448, 487, 958, 1038
CaiI CAGNNNCTG 3 cut(s) 34, 500, 947
CfoI GCGC 3 cut(s) 83, 97, 137
Cfr13I GGNCC 4 cut(s) 112, 120, 409, 944
Csp6I GTAC 4 cut(s) 23, 547, 1005, 1179
CviAII CATG 7 cut(s) 74, 127, 648, 688, 800, 846, 961
CviQI GTAC 4 cut(s) 23, 547, 1005, 1179
DdeI CTNAG 2 cut(s) 116, 1172
DpnI GATC 4 cut(s) 225, 397, 642, 1230
DpnII GATC 4 cut(s) 223, 395, 640, 1228
EaeI YGGCCR 1 cut(s) 375
EciI GGCGGA 2 cut(s) 112, 152
Eco130I CCWWGG 3 cut(s) 378, 465, 1158
Eco24I GRGCYC 2 cut(s) 173, 844
Eco47I GGWCC 2 cut(s) 120, 944
Eco57I CTGAAG 1 cut(s) 282
Eco81I CCTNAGG 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 906
EcoO109I RGGNCCY 2 cut(s) 112, 944
EcoRI GAATTC 2 cut(s) 304, 630
EcoRII CCWGG 2 cut(s) 165, 337
EcoT14I CCWWGG 3 cut(s) 378, 465, 1158
EcoT22I ATGCAT 1 cut(s) 801
EcoT38I GRGCYC 2 cut(s) 173, 844
ErhI CCWWGG 3 cut(s) 378, 465, 1158
FaeI CATG 7 cut(s) 77, 130, 651, 691, 803, 849, 964
FatI CATG 7 cut(s) 73, 126, 647, 687, 799, 845, 960
FauI CCCGC 1 cut(s) 455
FauNDI CATATG 1 cut(s) 452
FblI GTMKAC 1 cut(s) 1206
FokI GGATG 3 cut(s) 233, 311, 805
FriOI GRGCYC 2 cut(s) 173, 844
FspBI CTAG 2 cut(s) 38, 1028
GlaI GCGC 3 cut(s) 82, 96, 136
GsuI CTGGAG 2 cut(s) 897, 1221
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 4 cut(s) 113, 243, 377, 411
HapII CCGG 2 cut(s) 244, 1020
HhaI GCGC 3 cut(s) 83, 97, 137
Hin1II CATG 7 cut(s) 77, 130, 651, 691, 803, 849, 964
Hin6I GCGC 3 cut(s) 81, 95, 135
HinP1I GCGC 3 cut(s) 81, 95, 135
HinfI GANTC 1 cut(s) 57
HpaII CCGG 2 cut(s) 244, 1020
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 2 cut(s) 599, 1207
Hpy188I TCNGA 1 cut(s) 888
Hpy188III TCNNGA 8 cut(s) 232, 309, 617, 635, 908, 986, 1062, 1174
Hpy8I GTNNAC 2 cut(s) 599, 1207
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 3 cut(s) 103, 108, 434
HpyCH4III ACNGT 1 cut(s) 697
HpyF3I CTNAG 2 cut(s) 116, 1172
Hsp92II CATG 7 cut(s) 77, 130, 651, 691, 803, 849, 964
HspAI GCGC 3 cut(s) 81, 95, 135
Kzo9I GATC 4 cut(s) 223, 395, 640, 1228
LmnI GCTCC 7 cut(s) 320, 329, 382, 847, 980, 1051, 1150
Lsp1109I GCAGC 8 cut(s) 212, 352, 484, 610, 803, 914, 1087, 1109
LweI GCATC 7 cut(s) 178, 274, 556, 706, 743, 827, 1033
MaeI CTAG 2 cut(s) 38, 1028
MaeIII GTNAC 2 cut(s) 193, 514
MalI GATC 4 cut(s) 225, 397, 642, 1230
MboI GATC 4 cut(s) 223, 395, 640, 1228
MboII GAAGA 5 cut(s) 52, 53, 275, 418, 650
MflI RGATCY 1 cut(s) 395
MhlI GDGCHC 3 cut(s) 173, 448, 844
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 8 cut(s) 253, 304, 630, 729, 1000, 1167, 1184, 1224
MluNI TGGCCA 1 cut(s) 377
MmeI TCCRAC 3 cut(s) 126, 195, 307
Mox20I TGGCCA 1 cut(s) 377
Mph1103I ATGCAT 1 cut(s) 801
MscI TGGCCA 1 cut(s) 377
MseI TTAA 2 cut(s) 764, 1079
Msp20I TGGCCA 1 cut(s) 377
MspA1I CMGCKG 2 cut(s) 497, 1100
MspI CCGG 2 cut(s) 244, 1020
MspR9I CCNGG 3 cut(s) 167, 245, 339
MvaI CCWGG 2 cut(s) 167, 339
MvnI CGCG 2 cut(s) 95, 135
NciI CCSGG 1 cut(s) 245
NdeI CATATG 1 cut(s) 452
NdeII GATC 4 cut(s) 223, 395, 640, 1228
NlaIII CATG 7 cut(s) 77, 130, 651, 691, 803, 849, 964
NlaIV GGNNCC 4 cut(s) 397, 445, 742, 843
NmuCI GTSAC 1 cut(s) 193
NsiI ATGCAT 1 cut(s) 801
PaeR7I CTCGAG 1 cut(s) 906
PfeI GAWTC 1 cut(s) 57
PpuMI RGGWCCY 1 cut(s) 944
Psp5II RGGWCCY 1 cut(s) 944
Psp6I CCWGG 2 cut(s) 165, 337
PspGI CCWGG 2 cut(s) 165, 337
PspN4I GGNNCC 4 cut(s) 397, 445, 742, 843
PspPI GGNCC 4 cut(s) 112, 120, 409, 944
PspPPI RGGWCCY 1 cut(s) 944
PstI CTGCAG 1 cut(s) 202
PstNI CAGNNNCTG 3 cut(s) 34, 500, 947
PsuI RGATCY 1 cut(s) 395
PvuII CAGCTG 2 cut(s) 497, 1100
RsaI GTAC 4 cut(s) 24, 548, 1006, 1180
RsaNI GTAC 4 cut(s) 23, 547, 1005, 1179
SaqAI TTAA 2 cut(s) 764, 1079
Sau3AI GATC 4 cut(s) 223, 395, 640, 1228
Sau96I GGNCC 4 cut(s) 112, 120, 409, 944
ScrFI CCNGG 3 cut(s) 167, 245, 339
SduI GDGCHC 3 cut(s) 173, 448, 844
SfaNI GCATC 7 cut(s) 178, 274, 556, 706, 743, 827, 1033
SfcI CTRYAG 2 cut(s) 198, 696
Sfr274I CTCGAG 1 cut(s) 906
SinI GGWCC 2 cut(s) 120, 944
SlaI CTCGAG 1 cut(s) 906
SmlI CTYRAG 3 cut(s) 615, 745, 906
SmoI CTYRAG 3 cut(s) 615, 745, 906
Sse9I AATT 8 cut(s) 253, 304, 630, 729, 1000, 1167, 1184, 1224
SsiI CCGC 6 cut(s) 12, 123, 147, 150, 163, 448
SspMI CTAG 2 cut(s) 38, 1028
StyD4I CCNGG 3 cut(s) 165, 243, 337
StyI CCWWGG 3 cut(s) 378, 465, 1158
TaaI ACNGT 1 cut(s) 697
TaqI TCGA 1 cut(s) 907
TasI AATT 8 cut(s) 253, 304, 630, 729, 1000, 1167, 1184, 1224
TatI WGTACW 4 cut(s) 22, 546, 1004, 1178
TauI GCSGC 3 cut(s) 14, 149, 152
TfiI GAWTC 1 cut(s) 57
Tru1I TTAA 2 cut(s) 764, 1079
Tru9I TTAA 2 cut(s) 764, 1079
TscAI CASTG 2 cut(s) 202, 700
TseFI GTSAC 1 cut(s) 193
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 5 cut(s) 60, 62, 143, 905, 977
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 2 cut(s) 202, 700
VpaK11BI GGWCC 2 cut(s) 120, 944
XapI RAATTY 2 cut(s) 304, 630
XhoI CTCGAG 1 cut(s) 906
XmiI GTMKAC 1 cut(s) 1206
XspI CTAG 2 cut(s) 38, 1028
Zsp2I ATGCAT 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.