FvH4_7g13631

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004496
Physical Location & Seq
Forward (+)
12213491 .. 12217853
4363 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g13631.t2

Sequence Viewer

Length: 1341 bp
ATGAATAGCTTGTGGGAGAGAATCCGACAGTCTCAGGAAGACGACGACGAAGACGATATGATGTCTAACGCTATTGTGCTCGCGGCAGTTGCTGCCTCCCAAGCCCAACAACAACAACAGGAACTAGAAGATGAAAACGACAGATTCTTCGTTGCCGTTCATGTCGGCGCTGGATACCACGCGCCTTCCAACGAGAAGGCCCTCCGGTCCGCCATGAAACGCGCCTGCCTCGCCGCCGCTTCACTTCTCCGCCAGGGCTCTGGTAGATGCATAGATGCTGTAACTGCAGCTATCCAAGTGTTGGAGGATGATCCGAGCACGAATGCAGGCCGGGGGTCCAATCTGACAGAAGATGGGCGTGTGGAATGTGATGCCAGTATTATGGACGGGAATTCTGGAGCTTTTGGAGCTGTTGGAGCATTACCAGGTGTCAAAAACCCCATCAAGGTTGCTGCTTTATTGGCCAAGGAACAAATGATGGGATCTTCTCTGTTGGGCCGAATACCTCCCATTTTCCTAGTTGGTGAAGGTGCCCGCATATGGGCGAAGTCCAAGGGTATTGCTTTATCAGTAAGCACATCAGCTGCTGATGAGTGGCTGGTGACCGAAAGGGCAAAAGCACATTGGAAAAAGTACAAAGCAATGGTAGATGATGCAAACAAAAAAACAGAAACTTCTTCTGTTGGACTTTCTTTTAGTGCTCAAGATGGTTCTAGAATTCCTGAATTAGAAGCTCAACCATGTGATTCTTTGAATAAATACGGAGCAGAGGAGGATTGCATCATGGACACAGTCGGAGTTATTTGTGTAGATGCTGACGGACAAATTGCATCTGGTGCCTCAAGTGGAGGCATTGCATTAAAGGTTGGTGGTCGTGTGGGGTTAGCAGCAATGCATGGTTCAGGTTGTTGGGCATCCTCCAAAGGTCTCTTTGGGGCTCCATGTATAGTTGGCTGTTGTGCAAGTGGGGCTGGAGAATATTTGATGAAGGGATTTGCAGCTCGAGAGTGCTGTGTCTCTTTATCACTTTCACAGGCCGGTCCGGCCTCTGCTTGCATGAAAGTTTTACACTCTGTCGTTCAAGAGAGTAATCAGAATTGTACCGATAGAAGTGCCGGATTTCTTGTTGTGCAAGCTGATGCTCCCATGGTTTCAGGAAGTTCTCCAAAGTTAAAAGCTATTGAGATTGCAGCTGCTTACTCTACCTTATCCTTTGGGATAGGATATTTTGGAAGCTCTATGAAACGACCTAAGGCTTCAATTCTCAGGAGAACAAAATTGCAAAGCGAAACTGGAGTAGACCATTTTGAAGCACGAATTGATCTTTCAGCTAAAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

447

Amino Acids

46.7

Weight (kDa)

5.67

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 50 - 364 8.2e-49 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 530, 836
AccB7I CCANNNNNTGG 1 cut(s) 301
AccI GTMKAC 1 cut(s) 1299
AccII CGCG 3 cut(s) 83, 182, 222
AciI CCGC 6 cut(s) 83, 210, 234, 237, 250, 535
AclWI GGATC 2 cut(s) 305, 490
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 2 cut(s) 391, 717
AfaI GTAC 2 cut(s) 635, 1102
AfiI CCNNNNNNNGG 4 cut(s) 301, 445, 540, 541
AgsI TTSAA 4 cut(s) 754, 1082, 1260, 1310
AjnI CCWGG 2 cut(s) 252, 424
Alw21I GWGCWC 3 cut(s) 81, 320, 703
Alw26I GTCTC 3 cut(s) 36, 932, 1021
AlwI GGATC 2 cut(s) 305, 490
AlwNI CAGNNNCTG 2 cut(s) 92, 587
Ama87I CYCGRG 1 cut(s) 1002
AoxI GGCC 6 cut(s) 198, 328, 462, 496, 1035, 1044
ApeKI GCWGC 8 cut(s) 92, 287, 452, 584, 887, 998, 1190, 1193
ApoI RAATTY 2 cut(s) 391, 717
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspLEI GCGC 3 cut(s) 170, 184, 224
AspS9I GGNCC 5 cut(s) 199, 207, 336, 496, 1040
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 536, 613
AvaI CYCGRG 1 cut(s) 1002
AvaII GGWCC 3 cut(s) 207, 336, 1040
AxyI CCTNAGG 1 cut(s) 1251
BaeGI GKGCMC 1 cut(s) 535
BalI TGGCCA 1 cut(s) 464
BanI GGYRCC 2 cut(s) 530, 836
BanII GRGCYC 2 cut(s) 260, 940
BbsI GAAGAC 2 cut(s) 45, 57
Bbv12I GWGCWC 3 cut(s) 81, 320, 703
BbvI GCAGC 8 cut(s) 79, 299, 439, 571, 899, 1010, 1180, 1202
BccI CCATC 4 cut(s) 347, 449, 472, 701
BceAI ACGGC 1 cut(s) 140
BcgI CGANNNNNNTGC 2 cut(s) 1094, 1128
BciT130I CCWGG 2 cut(s) 254, 426
BciVI GTATCC 1 cut(s) 167
BcnI CCSGG 1 cut(s) 332
BcoDI GTCTC 3 cut(s) 36, 932, 1021
BfaI CTAG 3 cut(s) 125, 518, 714
BfmI CTRYAG 1 cut(s) 285
BfoI RGCGCY 1 cut(s) 171
BfuI GTATCC 1 cut(s) 167
BglI GCCNNNNNGGC 1 cut(s) 1043
Bme1390I CCNGG 3 cut(s) 254, 332, 426
Bme18I GGWCC 3 cut(s) 207, 336, 1040
BmeT110I CYCGRG 1 cut(s) 1002
BmgT120I GGNCC 5 cut(s) 199, 207, 336, 496, 1040
BmiI GGNNCC 4 cut(s) 337, 532, 838, 939
BmrFI CCNGG 3 cut(s) 254, 332, 426
BmsI GCATC 9 cut(s) 257, 265, 361, 643, 789, 802, 839, 923, 1129
BpiI GAAGAC 2 cut(s) 45, 57
BpmI CTGGAG 3 cut(s) 417, 993, 1314
BpuEI CTTGAG 2 cut(s) 687, 826
BpuMI CCSGG 1 cut(s) 332
BsaI GGTCTC 1 cut(s) 932
BsaJI CCNNGG 5 cut(s) 253, 331, 465, 552, 1146
BsaWI WCCGGW 1 cut(s) 204
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 445, 540, 541
Bse118I RCCGGY 1 cut(s) 1037
Bse1I ACTGG 2 cut(s) 375, 1297
Bse21I CCTNAGG 1 cut(s) 1251
Bse3DI GCAATG 3 cut(s) 648, 852, 897
BseBI CCWGG 2 cut(s) 254, 426
BseDI CCNNGG 5 cut(s) 253, 331, 465, 552, 1146
BseGI GGATG 2 cut(s) 313, 914
BseLI CCNNNNNNNGG 4 cut(s) 301, 445, 540, 541
BseMI GCAATG 3 cut(s) 648, 852, 897
BseMII CTCAG 2 cut(s) 47, 1279
BseNI ACTGG 2 cut(s) 375, 1297
BseRI GAGGAG 1 cut(s) 785
BseSI GKGCMC 1 cut(s) 535
BseXI GCAGC 8 cut(s) 79, 299, 439, 571, 899, 1010, 1180, 1202
Bsh1236I CGCG 3 cut(s) 83, 182, 222
BshFI GGCC 6 cut(s) 200, 330, 464, 498, 1037, 1046
BshNI GGYRCC 2 cut(s) 530, 836
BsiHKAI GWGCWC 3 cut(s) 81, 320, 703
BsiHKCI CYCGRG 1 cut(s) 1002
BsiSI CCGG 5 cut(s) 205, 331, 1038, 1043, 1116
BslI CCNNNNNNNGG 4 cut(s) 301, 445, 540, 541
BsmAI GTCTC 3 cut(s) 36, 932, 1021
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 6 cut(s) 200, 330, 464, 498, 1037, 1046
Bso31I GGTCTC 1 cut(s) 932
BsoBI CYCGRG 1 cut(s) 1002
Bsp1286I GDGCHC 6 cut(s) 81, 260, 320, 535, 703, 940
Bsp143I GATC 3 cut(s) 310, 482, 1321
Bsp19I CCATGG 1 cut(s) 1146
BspACI CCGC 6 cut(s) 83, 210, 234, 237, 250, 535
BspANI GGCC 6 cut(s) 200, 330, 464, 498, 1037, 1046
BspCNI CTCAG 2 cut(s) 46, 1278
BspFNI CGCG 3 cut(s) 83, 182, 222
BspLI GGNNCC 4 cut(s) 337, 532, 838, 939
BspMAI CTGCAG 1 cut(s) 289
BspPI GGATC 2 cut(s) 305, 490
BspT107I GGYRCC 2 cut(s) 530, 836
BspTNI GGTCTC 1 cut(s) 932
BsrDI GCAATG 3 cut(s) 648, 852, 897
BsrFI RCCGGY 1 cut(s) 1037
BsrI ACTGG 2 cut(s) 375, 1297
BssAI RCCGGY 1 cut(s) 1037
BssECI CCNNGG 5 cut(s) 253, 331, 465, 552, 1146
BssMI GATC 3 cut(s) 310, 482, 1321
BssT1I CCWWGG 3 cut(s) 465, 552, 1146
Bst2UI CCWGG 2 cut(s) 254, 426
Bst4CI ACNGT 2 cut(s) 30, 793
BstAPI GCANNNNNTGC 2 cut(s) 92, 836
BstC8I GCNNGC 6 cut(s) 81, 226, 328, 535, 1054, 1134
BstDEI CTNAG 3 cut(s) 33, 1251, 1265
BstDSI CCRYGG 1 cut(s) 1146
BstEII GGTNACC 1 cut(s) 601
BstF5I GGATG 2 cut(s) 313, 914
BstFNI CGCG 3 cut(s) 83, 182, 222
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 3 cut(s) 170, 184, 224
BstKTI GATC 3 cut(s) 313, 485, 1324
BstMAI GTCTC 3 cut(s) 36, 932, 1021
BstMBI GATC 3 cut(s) 310, 482, 1321
BstNI CCWGG 2 cut(s) 254, 426
BstPI GGTNACC 1 cut(s) 601
BstSCI CCNGG 3 cut(s) 252, 330, 424
BstSFI CTRYAG 1 cut(s) 285
BstSLI GKGCMC 1 cut(s) 535
BstUI CGCG 3 cut(s) 83, 182, 222
BstV1I GCAGC 8 cut(s) 79, 299, 439, 571, 899, 1010, 1180, 1202
BstV2I GAAGAC 2 cut(s) 45, 57
BstX2I RGATCY 1 cut(s) 482
BstXI CCANNNNNNTGG 2 cut(s) 260, 382
BstYI RGATCY 1 cut(s) 482
Bsu36I CCTNAGG 1 cut(s) 1251
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 6 cut(s) 200, 330, 464, 498, 1037, 1046
BtgI CCRYGG 1 cut(s) 1146
BtsCI GGATG 2 cut(s) 313, 914
Cac8I GCNNGC 6 cut(s) 81, 226, 328, 535, 1054, 1134
CaiI CAGNNNCTG 2 cut(s) 92, 587
CfoI GCGC 3 cut(s) 170, 184, 224
Cfr10I RCCGGY 1 cut(s) 1037
Cfr13I GGNCC 5 cut(s) 199, 207, 336, 496, 1040
CpoI CGGWCCG 2 cut(s) 207, 1040
CsiI ACCWGGT 1 cut(s) 424
Csp6I GTAC 2 cut(s) 634, 1101
CspI CGGWCCG 2 cut(s) 207, 1040
CviAII CATG 8 cut(s) 161, 214, 741, 784, 896, 942, 1057, 1147
CviQI GTAC 2 cut(s) 634, 1101
DdeI CTNAG 3 cut(s) 33, 1251, 1265
DpnI GATC 3 cut(s) 312, 484, 1323
DpnII GATC 3 cut(s) 310, 482, 1321
EaeI YGGCCR 1 cut(s) 462
EciI GGCGGA 2 cut(s) 199, 239
Eco130I CCWWGG 3 cut(s) 465, 552, 1146
Eco24I GRGCYC 2 cut(s) 260, 940
Eco31I GGTCTC 1 cut(s) 932
Eco47I GGWCC 3 cut(s) 207, 336, 1040
Eco81I CCTNAGG 1 cut(s) 1251
Eco88I CYCGRG 1 cut(s) 1002
Eco91I GGTNACC 1 cut(s) 601
EcoO109I RGGNCCY 1 cut(s) 199
EcoO65I GGTNACC 1 cut(s) 601
EcoRI GAATTC 2 cut(s) 391, 717
EcoRII CCWGG 2 cut(s) 252, 424
EcoT14I CCWWGG 3 cut(s) 465, 552, 1146
EcoT22I ATGCAT 2 cut(s) 272, 897
EcoT38I GRGCYC 2 cut(s) 260, 940
ErhI CCWWGG 3 cut(s) 465, 552, 1146
FaeI CATG 8 cut(s) 164, 217, 744, 787, 899, 945, 1060, 1150
FatI CATG 8 cut(s) 160, 213, 740, 783, 895, 941, 1056, 1146
FauI CCCGC 1 cut(s) 542
FauNDI CATATG 1 cut(s) 539
FblI GTMKAC 1 cut(s) 1299
FokI GGATG 2 cut(s) 320, 901
FriOI GRGCYC 2 cut(s) 260, 940
FspBI CTAG 3 cut(s) 125, 518, 714
GlaI GCGC 3 cut(s) 169, 183, 223
GsuI CTGGAG 3 cut(s) 417, 993, 1314
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 6 cut(s) 200, 330, 464, 498, 1037, 1046
HapII CCGG 5 cut(s) 205, 331, 1038, 1043, 1116
HhaI GCGC 3 cut(s) 170, 184, 224
Hin1II CATG 8 cut(s) 164, 217, 744, 787, 899, 945, 1060, 1150
Hin6I GCGC 3 cut(s) 168, 182, 222
HinP1I GCGC 3 cut(s) 168, 182, 222
HinfI GANTC 3 cut(s) 21, 144, 746
HpaII CCGG 5 cut(s) 205, 331, 1038, 1043, 1116
HphI GGTGA 2 cut(s) 536, 613
Hpy166II GTNNAC 1 cut(s) 1300
Hpy188I TCNGA 5 cut(s) 26, 315, 345, 797, 1095
Hpy188III TCNNGA 9 cut(s) 35, 396, 704, 714, 722, 1004, 1082, 1155, 1267
Hpy8I GTNNAC 1 cut(s) 1300
Hpy99I CGWCG 2 cut(s) 47, 50
HpyAV CCTTC 4 cut(s) 190, 195, 521, 982
HpyCH4III ACNGT 2 cut(s) 30, 793
HpyF3I CTNAG 3 cut(s) 33, 1251, 1265
Hsp92II CATG 8 cut(s) 164, 217, 744, 787, 899, 945, 1060, 1150
HspAI GCGC 3 cut(s) 168, 182, 222
Kzo9I GATC 3 cut(s) 310, 482, 1321
LmnI GCTCC 6 cut(s) 398, 407, 416, 764, 943, 1147
Lsp1109I GCAGC 8 cut(s) 79, 299, 439, 571, 899, 1010, 1180, 1202
LweI GCATC 9 cut(s) 257, 265, 361, 643, 789, 802, 839, 923, 1129
MabI ACCWGGT 1 cut(s) 424
MaeI CTAG 3 cut(s) 125, 518, 714
MaeIII GTNAC 2 cut(s) 280, 601
MalI GATC 3 cut(s) 312, 484, 1323
MboI GATC 3 cut(s) 310, 482, 1321
MboII GAAGA 7 cut(s) 50, 62, 139, 140, 362, 477, 669
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 6 cut(s) 81, 260, 320, 535, 703, 940
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 8 cut(s) 391, 717, 725, 825, 1096, 1260, 1277, 1317
MluNI TGGCCA 1 cut(s) 464
MmeI TCCRAC 6 cut(s) 49, 213, 282, 394, 664, 775
Mox20I TGGCCA 1 cut(s) 464
Mph1103I ATGCAT 2 cut(s) 272, 897
MscI TGGCCA 1 cut(s) 464
MseI TTAA 2 cut(s) 860, 1172
Msp20I TGGCCA 1 cut(s) 464
MspA1I CMGCKG 2 cut(s) 584, 1193
MspI CCGG 5 cut(s) 205, 331, 1038, 1043, 1116
MspR9I CCNGG 3 cut(s) 254, 332, 426
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 2 cut(s) 254, 426
MvnI CGCG 3 cut(s) 83, 182, 222
NciI CCSGG 1 cut(s) 332
NcoI CCATGG 1 cut(s) 1146
NdeI CATATG 1 cut(s) 539
NdeII GATC 3 cut(s) 310, 482, 1321
NlaIII CATG 8 cut(s) 164, 217, 744, 787, 899, 945, 1060, 1150
NlaIV GGNNCC 4 cut(s) 337, 532, 838, 939
NmuCI GTSAC 1 cut(s) 601
NsiI ATGCAT 2 cut(s) 272, 897
PaeR7I CTCGAG 1 cut(s) 1002
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 1 cut(s) 328
PfeI GAWTC 3 cut(s) 21, 144, 746
PflFI GACNNNGTC 1 cut(s) 791
PflMI CCANNNNNTGG 1 cut(s) 301
Psp6I CCWGG 2 cut(s) 252, 424
PspEI GGTNACC 1 cut(s) 601
PspGI CCWGG 2 cut(s) 252, 424
PspN4I GGNNCC 4 cut(s) 337, 532, 838, 939
PspPI GGNCC 5 cut(s) 199, 207, 336, 496, 1040
PstI CTGCAG 1 cut(s) 289
PstNI CAGNNNCTG 2 cut(s) 92, 587
PsuI RGATCY 1 cut(s) 482
PsyI GACNNNGTC 1 cut(s) 791
PvuII CAGCTG 2 cut(s) 584, 1193
RsaI GTAC 2 cut(s) 635, 1102
RsaNI GTAC 2 cut(s) 634, 1101
Rsr2I CGGWCCG 2 cut(s) 207, 1040
RsrII CGGWCCG 2 cut(s) 207, 1040
SaqAI TTAA 2 cut(s) 860, 1172
Sau3AI GATC 3 cut(s) 310, 482, 1321
Sau96I GGNCC 5 cut(s) 199, 207, 336, 496, 1040
ScrFI CCNGG 3 cut(s) 254, 332, 426
SduI GDGCHC 6 cut(s) 81, 260, 320, 535, 703, 940
SexAI ACCWGGT 1 cut(s) 424
SfaNI GCATC 9 cut(s) 257, 265, 361, 643, 789, 802, 839, 923, 1129
SfcI CTRYAG 1 cut(s) 285
SfiI GGCCNNNNNGGCC 1 cut(s) 1043
Sfr274I CTCGAG 1 cut(s) 1002
SinI GGWCC 3 cut(s) 207, 336, 1040
SlaI CTCGAG 1 cut(s) 1002
SmlI CTYRAG 3 cut(s) 702, 841, 1002
SmoI CTYRAG 3 cut(s) 702, 841, 1002
Sse9I AATT 8 cut(s) 391, 717, 725, 825, 1096, 1260, 1277, 1317
SsiI CCGC 6 cut(s) 83, 210, 234, 237, 250, 535
SspI AATATT 1 cut(s) 980
SspMI CTAG 3 cut(s) 125, 518, 714
StyD4I CCNGG 3 cut(s) 252, 330, 424
StyI CCWWGG 3 cut(s) 465, 552, 1146
TaaI ACNGT 2 cut(s) 30, 793
TaqI TCGA 1 cut(s) 1003
TaqII GACCGA 1 cut(s) 620
TasI AATT 8 cut(s) 391, 717, 725, 825, 1096, 1260, 1277, 1317
TatI WGTACW 1 cut(s) 633
TauI GCSGC 3 cut(s) 86, 236, 239
TfiI GAWTC 3 cut(s) 21, 144, 746
Tru1I TTAA 2 cut(s) 860, 1172
Tru9I TTAA 2 cut(s) 860, 1172
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 8 cut(s) 92, 287, 452, 584, 887, 998, 1190, 1193
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 7 cut(s) 17, 147, 149, 230, 1001, 1073, 1256
TspGWI ACGGA 2 cut(s) 777, 834
Tth111I GACNNNGTC 1 cut(s) 791
Van91I CCANNNNNTGG 1 cut(s) 301
VpaK11BI GGWCC 3 cut(s) 207, 336, 1040
XapI RAATTY 2 cut(s) 391, 717
XbaI TCTAGA 1 cut(s) 713
XhoI CTCGAG 1 cut(s) 1002
XmiI GTMKAC 1 cut(s) 1299
XspI CTAG 3 cut(s) 125, 518, 714
Zsp2I ATGCAT 2 cut(s) 272, 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.